added isPcr information to manuscript

dr-joe-wirth · Jul 30, 2024 · 7b0044b · 7b0044b
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diff --git a/paper/paper.bib b/paper/paper.bib
@@ -21,3 +21,16 @@ @article{10.1093/nar/gks596
     doi = {10.1093/nar/gks596},
     url = {https://doi.org/10.1093/nar/gks596}
 }
+
+@article{10.1093/bib/bbs038,
+    author = {{Khun}, Robert M. and {Haussler}, David and {Kent}, James},
+    title = "{The UCSC genome browser and associated tools}",
+    journal = {Briefings in Bioinformatics},
+    volume = {14},
+    number = {2},
+    year = {2012},
+    issn = {1467-5463},
+    doi = {10.1093/bib/bbs038},
+    url = {https://doi.org/10.1093/bib/bbs038}
+}
+}
diff --git a/paper/paper.md b/paper/paper.md
@@ -62,7 +62,9 @@ from another set of genomes via PCR amplification. `primerForge` relies on the
 `khmer` package to extract k-mers from genomic sequences and the `primer3-py`
 package to evaluate specific characteristics of primer pairs including melting
 temperature, hairpin potential, and dimer formation
-[@10.12688/f1000research.6924.1; @10.1093/nar/gks596].
+[@10.12688/f1000research.6924.1; @10.1093/nar/gks596]. It also uses _in silico_
+PCR via the `isPcr` program to validate and filter the primer pairs
+[@10.1093/bib/bbs038].
 
 There are many use cases for what `primerForge` offers. One use case would be
 surveillance of an outbreak clone of a particular pathogen. A laboratory could