Merge pull request #197 from nf-core/dev

Release PR for 2.1.0
nf-core · Oct 24, 2022 · 83fe0b6 · 83fe0b6
2 parents d1299b1 + 5f84f21
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diff --git a/.editorconfig b/.editorconfig
@@ -8,7 +8,7 @@ trim_trailing_whitespace = true
 indent_size = 4
 indent_style = space
 
-[*.{md,yml,yaml,html,css,scss,js}]
+[*.{md,yml,yaml,html,css,scss,js,cff}]
 indent_size = 2
 
 # These files are edited and tested upstream in nf-core/modules

diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
@@ -15,8 +15,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/smrn
 
 - [ ] This comment contains a description of changes (with reason).
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
-  - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md)
-  - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
+- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md)- [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
@@ -25,3 +25,7 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/smrnaseq/results-${{ github.sha }}"
             }
           profiles: test_full,aws_tower
+      - uses: actions/upload-artifact@v3
+        with:
+          name: Tower debug log file
+          path: tower_action_*.log
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
@@ -23,3 +23,7 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/smrnaseq/results-test-${{ github.sha }}"
             }
           profiles: test,aws_tower
+      - uses: actions/upload-artifact@v3
+        with:
+          name: Tower debug log file
+          path: tower_action_*.log
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
@@ -10,7 +10,6 @@ on:
 
 env:
   NXF_ANSI_LOG: false
-  CAPSULE_LOG: none
 
 jobs:
   test:
@@ -20,29 +19,21 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        # Nextflow versions
-        include:
-          # Test pipeline minimum Nextflow version
-          - NXF_VER: "21.10.3"
-            NXF_EDGE: ""
-          # Test latest edge release of Nextflow
-          - NXF_VER: ""
-            NXF_EDGE: "1"
+        NXF_VER:
+          - "21.10.3"
+          - "latest-everything"
+        profile:
+          - "test"
+          - "test_no_genome"
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
 
       - name: Install Nextflow
-        env:
-          NXF_VER: ${{ matrix.NXF_VER }}
-          # Uncomment only if the edge release is more recent than the latest stable release
-          # See https://github.com/nextflow-io/nextflow/issues/2467
-          # NXF_EDGE: ${{ matrix.NXF_EDGE }}
-        run: |
-          wget -qO- get.nextflow.io | bash
-          sudo mv nextflow /usr/local/bin/
+        uses: nf-core/setup-nextflow@v1
+        with:
+          version: "${{ matrix.NXF_VER }}"
 
       - name: Run pipeline with test data
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results
-          nextflow run ${GITHUB_WORKSPACE} -profile test_no_genome,docker --outdir ./results
+          nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.profile }},docker --outdir ./results
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
@@ -35,28 +35,55 @@ jobs:
       - name: Run Prettier --check
         run: prettier --check ${GITHUB_WORKSPACE}
 
+  PythonBlack:
+    runs-on: ubuntu-latest
+    steps:
+      - uses: actions/checkout@v2
+
+      - name: Check code lints with Black
+        uses: psf/black@stable
+
+      # If the above check failed, post a comment on the PR explaining the failure
+      - name: Post PR comment
+        if: failure()
+        uses: mshick/add-pr-comment@v1
+        with:
+          message: |
+            ## Python linting (`black`) is failing
+
+            To keep the code consistent with lots of contributors, we run automated code consistency checks.
+            To fix this CI test, please run:
+
+            * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black`
+            * Fix formatting errors in your pipeline: `black .`
+
+            Once you push these changes the test should pass, and you can hide this comment :+1:
+
+            We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help!
+
+            Thanks again for your contribution!
+          repo-token: ${{ secrets.GITHUB_TOKEN }}
+          allow-repeats: false
+
   nf-core:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
 
       - name: Install Nextflow
-        env:
-          CAPSULE_LOG: none
-        run: |
-          wget -qO- get.nextflow.io | bash
-          sudo mv nextflow /usr/local/bin/
+        uses: nf-core/setup-nextflow@v1
 
       - uses: actions/setup-python@v3
         with:
-          python-version: "3.6"
+          python-version: "3.7"
           architecture: "x64"
 
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
           pip install nf-core
+
       - name: Run nf-core lint
         env:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}

diff --git a/.nf-core.yml b/.nf-core.yml
@@ -1,8 +1 @@
 repository_type: pipeline
-lint:
-  files_unchanged:
-    - .github/workflows/linting.yml
-    - .github/workflows/branch.yml
-    - .github/workflows/ci.yml
-    - .github/workflows/linting.yml
-    - .github/workflows/linting_comment.yml
diff --git a/.prettierignore b/.prettierignore
@@ -1,4 +1,5 @@
 email_template.html
+adaptivecard.json
 .nextflow*
 work/
 data/

diff --git a/CHANGELOG.md b/CHANGELOG.md
@@ -3,6 +3,37 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## [v2.1.0](https://github.com/nf-core/smrnaseq/releases/tag/2.1.0) - 2022-10-20 Maroon Tin Dalmatian
+
+### Enhancements & fixes
+
+- [[#12](https://github.com/nf-core/smrnaseq/issues/12)] - Enabled the use of `MirGeneDB` as an alternative database insted of `miRBase`
+- [[#113](https://github.com/nf-core/smrnaseq/issues/113)] - Added a optional contamination filtering step, including MultiQC plot
+- [[#137](https://github.com/nf-core/smrnaseq/issues/137)] - Fixed issue with mirTop and MultiQC by upgrading to MultiQC V1.13dev
+- [[#159](https://github.com/nf-core/smrnaseq/issues/159)] - Index files were not collected when `bowtie_index` was used and thus mapping was failing
+- [[#161](https://github.com/nf-core/smrnaseq/issues/161)] - Trimmed output was not as documented and not correctly published
+- [[#168](https://github.com/nf-core/smrnaseq/issues/168)] - Removed `mirtrace_protocol` as the parameter was redundant and `params.protocol` is entirely sufficient
+- Updated pipeline template to [nf-core/tools 2.6.0](https://github.com/nf-core/tools/releases/tag/2.6.0)
+- [[#188](https://github.com/nf-core/smrnaseq/pull/188)] - Dropped TrimGalore in favor of fastp QC and adapter trimming, improved handling of adapters and trimming parameters
+- [[#194](https://github.com/nf-core/smrnaseq/issues/194)] - Added default adapters file for FastP improved miRNA adapter trimming
+
+### Parameters
+
+| Old parameter | New parameter            |
+| ------------- | ------------------------ |
+|               | `--mirgenedb`            |
+|               | `--mirgenedb_species`    |
+|               | `--mirgenedb_gff`        |
+|               | `--mirgenedb_mature`     |
+|               | `--mirgenedb_hairpin`    |
+|               | `--contamination_filter` |
+|               | `--rrna`                 |
+|               | `--trna`                 |
+|               | `--cdna`                 |
+|               | `--ncrna`                |
+|               | `--pirna`                |
+|               | `--other_contamination`  |
+
 ## [v2.0.0](https://github.com/nf-core/smrnaseq/releases/tag/2.0.0) - 2022-05-31 Aqua Zinc Chihuahua
 
 ### Major enhancements
@@ -24,10 +55,11 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Parameters
 
-| Old parameter      | New parameter    |
-| ------------------ | ---------------- |
-| `--conda`          | `--enable_conda` |
-| `--clusterOptions` |                  |
+| Old parameter        | New parameter    |
+| -------------------- | ---------------- |
+| `--conda`            | `--enable_conda` |
+| `--clusterOptions`   |                  |
+| `--publish_dir_mode` |                  |
 
 > **NB:** Parameter has been **updated** if both old and new parameter information is present.
 > **NB:** Parameter has been **added** if just the new parameter information is present.
@@ -59,6 +91,8 @@ Note, since the pipeline is now using Nextflow DSL2, each process will be run wi
 | `pymdown-extensions` | -           | -           |
 | `pygments`           | -           | -           |
 | `r-r.methodss3`      | -           | -           |
+| `bowtie2`            | -           | 2.4.5       |
+| `blat`               | -           | 36          |
 
 > **NB:** Dependency has been **updated** if both old and new version information is present.
 > **NB:** Dependency has been **added** if just the new version information is present.

diff --git a/CITATION.cff b/CITATION.cff
@@ -0,0 +1,56 @@
+cff-version: 1.2.0
+message: "If you use `nf-core tools` in your work, please cite the `nf-core` publication"
+authors:
+  - family-names: Ewels
+    given-names: Philip
+  - family-names: Peltzer
+    given-names: Alexander
+  - family-names: Fillinger
+    given-names: Sven
+  - family-names: Patel
+    given-names: Harshil
+  - family-names: Alneberg
+    given-names: Johannes
+  - family-names: Wilm
+    given-names: Andreas
+  - family-names: Garcia
+    given-names: Maxime Ulysse
+  - family-names: Di Tommaso
+    given-names: Paolo
+  - family-names: Nahnsen
+    given-names: Sven
+title: "The nf-core framework for community-curated bioinformatics pipelines."
+version: 2.4.1
+doi: 10.1038/s41587-020-0439-x
+date-released: 2022-05-16
+url: https://github.com/nf-core/tools
+prefered-citation:
+  type: article
+  authors:
+    - family-names: Ewels
+      given-names: Philip
+    - family-names: Peltzer
+      given-names: Alexander
+    - family-names: Fillinger
+      given-names: Sven
+    - family-names: Patel
+      given-names: Harshil
+    - family-names: Alneberg
+      given-names: Johannes
+    - family-names: Wilm
+      given-names: Andreas
+    - family-names: Garcia
+      given-names: Maxime Ulysse
+    - family-names: Di Tommaso
+      given-names: Paolo
+    - family-names: Nahnsen
+      given-names: Sven
+  doi: 10.1038/s41587-020-0439-x
+  journal: nature biotechnology
+  start: 276
+  end: 278
+  title: "The nf-core framework for community-curated bioinformatics pipelines."
+  issue: 3
+  volume: 38
+  year: 2020
+  url: https://dx.doi.org/10.1038/s41587-020-0439-x
diff --git a/LICENSE b/LICENSE
@@ -1,6 +1,6 @@
 MIT License
 
-Copyright (c) P. Ewels <[email protected]>, C. Wang <[email protected]>, R. Hammarén <[email protected]>, L. Pantano <[email protected]>
+Copyright (c) P. Ewels, C. Wang, R. Hammarén, L. Pantano
 
 Permission is hereby granted, free of charge, to any person obtaining a copy
 of this software and associated documentation files (the "Software"), to deal

diff --git a/README.md b/README.md
@@ -1,21 +1,14 @@
 # ![nf-core/smrnaseq](docs/images/nf-core-smrnaseq_logo_light.png#gh-light-mode-only) ![nf-core/smrnaseq](docs/images/nf-core-smrnaseq_logo_dark.png#gh-dark-mode-only)
 
-[![GitHub Actions CI Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/smrnaseq/actions?query=workflow%3A%22nf-core+CI%22)
-[![GitHub Actions Linting Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/smrnaseq/actions?query=workflow%3A%22nf-core+linting%22)
-[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?logo=Amazon%20AWS)](https://nf-co.re/smrnaseq/results)
-[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8)](https://doi.org/10.5281/zenodo.4956678)
+[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/smrnaseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3456879-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3456879)
 
 [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg)](https://www.nextflow.io/)
-[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?logo=anaconda)](https://docs.conda.io/en/latest/)
-[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?logo=docker)](https://www.docker.com/)
-[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg)](https://sylabs.io/docs/)
+[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
+[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
+[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
 [![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/smrnaseq)
 
-[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23smrnaseq-4A154B?logo=slack)](https://nfcore.slack.com/channels/smrnaseq)
-[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?logo=twitter)](https://twitter.com/nf_core)
-[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?logo=youtube)](https://www.youtube.com/c/nf-core)
-
-[![DOI](https://zenodo.org/badge/140590861.svg)](https://zenodo.org/badge/latestdoi/140590861)
+[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23smrnaseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/smrnaseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
 
 ## Introduction
 
@@ -25,30 +18,37 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 
 On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/smrnaseq/results).
 
+## Online videos
+
+A short talk about the history, current status and functionality on offer in this pipeline was given by Lorena Pantano (@lpantano) on [9th November 2021](https://youtu.be/4YLQ2VwpCJE) as part of the nf-core/bytesize series.
+
+You can find numerous talks on the nf-core events page from various topics including writing pipelines/modules in Nextflow DSL2, using nf-core tooling, running nf-core pipelines as well as more generic content like contributing to Github. Please check them out!
+
 ## Pipeline summary
 
 1. Raw read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
 2. Adapter trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/))
    1. Insert Size calculation
    2. Collapse reads ([`seqcluster`](https://seqcluster.readthedocs.io/mirna_annotation.html#processing-of-reads))
-3. Alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-4. Alignment against miRBase hairpin
+3. Contamination filtering ([`Bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml))
+4. Alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
+5. Alignment against miRBase hairpin
    1. Unaligned reads from step 3 ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
    2. Collapsed reads from step 2.2 ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-5. Post-alignment processing of miRBase hairpin
+6. Post-alignment processing of miRBase hairpin
    1. Basic statistics from step 3 and step 4.1 ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
-   2. Analysis on miRBase hairpin counts ([`edgeR`](https://bioconductor.org/packages/release/bioc/html/edgeR.html))
+   2. Analysis on miRBase, or MirGeneDB hairpin counts ([`edgeR`](https://bioconductor.org/packages/release/bioc/html/edgeR.html))
       - TMM normalization and a table of top expression hairpin
       - MDS plot clustering samples
       - Heatmap of sample similarities
    3. miRNA and isomiR annotation from step 4.1 ([`mirtop`](https://github.com/miRTop/mirtop))
-6. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
+7. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
    1. Post-alignment processing of alignment against host reference genome ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
-7. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation))
+8. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation))
    1. Mapping against reference genome with the mapper module
    2. Known and novel miRNA discovery with the mirdeep2 module
-8. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace))
-9. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
+9. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace))
+10. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
 
 ## Quick Start
 
@@ -58,7 +58,7 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 3. Download the pipeline and test it on a minimal dataset with a single command:
 
-   ```console
+   ```bash
    nextflow run nf-core/smrnaseq -profile test,YOURPROFILE --outdir <OUTDIR>
    ```
 
@@ -71,7 +71,7 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 4. Start running your own analysis!
 
-   ```console
+   ```bash
    nextflow run nf-core/smrnaseq --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
    ```