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Do you first need sequences of each individual from a community or can you sequence 50 species and provide the 50 reference sequences?
$ bonito basecaller dna_r9.4.1 --save-ctc --reference reference.mmi /data/reads > /data/training/ctc-data/basecalls.sam
Do you put individual species together to train or ..?
$ bonito train --directory /data/training/ctc-data /data/training/model-dir
Can you give an example how to train a model for example fish species (50 fish species)?
With for simplified reasons the folowing 3 fish: sole / tuna / salmon.
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Do you first need sequences of each individual from a community or can you sequence 50 species and provide the 50 reference sequences?
$ bonito basecaller dna_r9.4.1 --save-ctc --reference reference.mmi /data/reads > /data/training/ctc-data/basecalls.sam
Do you put individual species together to train or ..?
$ bonito train --directory /data/training/ctc-data /data/training/model-dir
Can you give an example how to train a model for example fish species (50 fish species)?
With for simplified reasons the folowing 3 fish: sole / tuna / salmon.
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