acadie_16s

Analysis of the phyllosphere and soil bacterial communities

Data type: Miseq 16S amplicon sequencing data

Note 1: Due to the high number of samples, different sampling sites and several years of sampling, the samples have been randomly sequenced in multiple sequencing runs.

Note 2: The present analyses have been performed on demultiplexed sequence data of only one sampling site (ACA).

Steps of data analysis:

• DADA2 analysis of different sequening runs individually
• Merge the final results of multiple runs and assign taxonomy
• Quality control, denoising and cleaning data
• Explanatory analysis of the non-treated (control) samples
• Explanatory analysis of the phyllosphere samples
• Differential expression analysis of the phyllosphere samples
• Explanatory analysis of the soil samples
• Differential expression analysis of the soil samples