Please refer to the following preprint:

de Vito, Giuseppe, et al. "Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light sheet microscopy." arXiv preprint arXiv:2012.09660 (2020).

How to install and use

sudo apt install python3-tk
pip3 install -r requirements.txt

Print help:

python3 affine-alignment.py -h