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file name fix
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@y3rsh
y3rsh committed Aug 29, 2024
1 parent 40776f3 commit a68a093
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0 ...S_2_20_8_None_PARTIAL_COLUMN_HappyPath.py → ..._v2_20_8_None_PARTIAL_COLUMN_HappyPath.py
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0 ...ls/Flex_S_2_20_8_None_SINGLE_HappyPath.py → ...s/Flex_S_v2_20_8_None_SINGLE_HappyPath.py
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296 changes: 296 additions & 0 deletions analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_AllCorners.py
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from opentrons import protocol_api
from opentrons.protocol_api import COLUMN, SINGLE, ROW

requirements = {"robotType": "Flex", "apiLevel": "2.20"}


def run(protocol: protocol_api.ProtocolContext):
pipette = protocol.load_instrument(instrument_name="flex_96channel_1000")
trash = protocol.load_trash_bin("B3")
t1 = protocol.load_labware(
load_name="opentrons_flex_96_tiprack_1000ul",
label="A1 Corner Tiprack 1",
location="B1",
)
t2 = protocol.load_labware(
load_name="opentrons_flex_96_tiprack_1000ul",
label="D1 Corner Tiprack",
location="C1",
)
t3 = protocol.load_labware(
load_name="opentrons_flex_96_tiprack_1000ul",
label="A3 Corner Tiprack 1",
location="B2",
)
t4 = protocol.load_labware(
load_name="opentrons_flex_96_tiprack_1000ul",
label="D3 Corner Tiprack",
location="C2",
)
t5 = protocol.load_labware(
load_name="opentrons_flex_96_tiprack_1000ul",
label="A3 Corner Tiprack 2",
location="A2",
)
t6 = protocol.load_labware(
load_name="opentrons_flex_96_tiprack_1000ul",
label="A1 Corner Tiprack 2",
location="D4",
)

### SETUP TIPRACK FUNCTIONS
# These functions serve the purpose of removing tips from a tiprack before it is moved to a corner slot
# This is done to ensure the tiprack is in such a state that it will trigger zero deck extent conflicts

# Setup T2
def t2_setup() -> None:
pipette.configure_nozzle_layout(
style=ROW,
start="A1",
tip_racks=[t2],
)
for i in range(3):
pipette.pick_up_tip()
pipette.drop_tip()

# Setup T3
def t3_setup() -> None:
pipette.configure_nozzle_layout(style=COLUMN, start="A1", tip_racks=[t3])
for i in range(2):
pipette.pick_up_tip()
pipette.drop_tip()

# Setup T4
def t4_setup() -> None:
pipette.configure_nozzle_layout(
style=ROW,
start="A1",
tip_racks=[t4],
)
for i in range(3):
pipette.pick_up_tip()
pipette.drop_tip()

pipette.configure_nozzle_layout(
style=SINGLE,
start="A1",
tip_racks=[t4],
)
for i in range(10):
pipette.pick_up_tip()
pipette.drop_tip()

# Setup T5
def t5_setup() -> None:
pipette.configure_nozzle_layout(
style=ROW,
start="H1",
tip_racks=[t5],
)
for i in range(7):
pipette.pick_up_tip()
pipette.drop_tip()

pipette.configure_nozzle_layout(
style=SINGLE,
start="A1",
tip_racks=[t5],
)
for i in range(2):
pipette.pick_up_tip()
pipette.drop_tip()

# Setup T6
def t6_setup() -> None:
pipette.configure_nozzle_layout(
style=ROW,
start="H1",
tip_racks=[t6],
)
for i in range(7):
pipette.pick_up_tip()
pipette.drop_tip()

### PICKUP TIP FUNCTIONS
# These functions perform pickup tip behavior for a given tiprack

# Pickup T2
def t1_pickup() -> None:
pipette.configure_nozzle_layout(
style=SINGLE,
start="A1",
tip_racks=[t1],
)
for i in range(48):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="A12",
tip_racks=[t1],
)
for i in range(48):
pipette.pick_up_tip()
pipette.drop_tip()

# Pickup T2
def t2_pickup() -> None:
pipette.configure_nozzle_layout(
style=SINGLE,
start="A1",
tip_racks=[t2],
)
for i in range(15):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="A12",
tip_racks=[t2],
)
for i in range(15):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="H1",
tip_racks=[t2],
)
for i in range(15):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="H12",
tip_racks=[t2],
)
for i in range(15):
pipette.pick_up_tip()
pipette.drop_tip()

# Pickup T3
def t3_pickup() -> None:
pipette.configure_nozzle_layout(
style=SINGLE,
start="A1",
tip_racks=[t3],
)
for i in range(40):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="A12",
tip_racks=[t3],
)
for i in range(40):
pipette.pick_up_tip()
pipette.drop_tip()

# Pickup T4
def t4_pickup() -> None:
pipette.configure_nozzle_layout(
style=SINGLE,
start="A1",
tip_racks=[t4],
)
for i in range(10):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="A12",
tip_racks=[t4],
)
for i in range(10):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="H1",
tip_racks=[t4],
)
for i in range(15):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="H12",
tip_racks=[t4],
)
for i in range(15):
pipette.pick_up_tip()
pipette.drop_tip()

# Pickup T5
def t5_pickup() -> None:
pipette.configure_nozzle_layout(
style=SINGLE,
start="H1",
tip_racks=[t5],
)
for i in range(5):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="H12",
tip_racks=[t5],
)
for i in range(5):
pipette.pick_up_tip()
pipette.drop_tip()

# Pickup T6
def t6_pickup() -> None:
pipette.configure_nozzle_layout(
style=SINGLE,
start="H1",
tip_racks=[t6],
)
for i in range(6):
pipette.pick_up_tip()
pipette.drop_tip()
pipette.configure_nozzle_layout(
style=SINGLE,
start="H12",
tip_racks=[t6],
)
for i in range(6):
pipette.pick_up_tip()
pipette.drop_tip()

### Protocol Actions
# Perform Setup of first set of tipracks
protocol.move_labware(t1, "A1", True)
t2_setup()
protocol.move_labware(t2, "D1", True)
t3_setup()
protocol.move_labware(t3, "A3", True)
t4_setup()
protocol.move_labware(t4, "D3", True)

# Clear out T2 first to make room for T5
t2_pickup()
protocol.move_labware(t5, "B2", True)
t5_setup()
protocol.move_labware(t5, "C1", True)

# Clear out T1, T3, and T4
t1_pickup()
t3_pickup()
protocol.move_labware(t6, "A4", True)
t4_pickup()
protocol.move_labware(t6, "D4", True)

# Move T3, then move and handle T5
protocol.move_labware(t3, "D2", True)
protocol.move_labware(t5, "A3", True)
t5_pickup()

# Move T6 to On-Deck in B2 and setup, then move to A1 and handle
protocol.move_labware(t6, "B2", True)
t6_setup()
protocol.move_labware(t1, "C3", True)
protocol.move_labware(t6, "A1", True)
t6_pickup()
0 ...s/Flex_S_2_20_96_None_COLUMN_HappyPath.py → .../Flex_S_v2_20_96_None_COLUMN_HappyPath.py
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0 ...ls/Flex_S_2_20_96_None_Column3_SINGLE_.py → ...s/Flex_S_v2_20_96_None_Column3_SINGLE_.py
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0 ...cols/Flex_S_2_20_96_None_ROW_HappyPath.py → ...ols/Flex_S_v2_20_96_None_ROW_HappyPath.py
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0 ...2_20_96_None_SINGLE_HappyPathNorthSide.py → ...2_20_96_None_SINGLE_HappyPathNorthSide.py
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0 ...2_20_96_None_SINGLE_HappyPathSouthSide.py → ...2_20_96_None_SINGLE_HappyPathSouthSide.py
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0 ..._PARTIAL_COLUMN_HappyPathMixedTipRacks.py → ..._PARTIAL_COLUMN_HappyPathMixedTipRacks.py
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0 ...ols/OT2_S_2_20_8_None_SINGLE_HappyPath.py → ...ls/OT2_S_v2_20_8_None_SINGLE_HappyPath.py
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