Tumors-produce-glucocorticoids-by-metabolite-recycling

This code accompanies the paper entitled:

Taves MD, Otsuka S, Taylor MA, Donahue KM, Meyer TJ, Cam MC, Ashwell JD. Tumors produce glucocorticoids by metabolite recycling, not synthesis, and activate Tregs to promote growth. J Clin Invest. 2023 Sep 15;133(18):e164599. doi: 10.1172/JCI164599. PMID: 37471141; PMCID: PMC10503810. Link

To reproduce these results, follow these steps:

1. Clone this GitHub repo (i.e. the page you are on):

   • git clone https://github.com/NIDAP-Community/Tumors-produce-glucocorticoids-by-metabolite-recycling.git

2. The input files for this pipeline will be available upon request. Please reach out to the authors before continue to following steps

3. Install docker and build the docker container:

   • Navigate to the cloned repository directory.
   • Move to the ./Docker_file/ directory of this repo

4. Build the container:

   • docker build --tag Tumors-produce-glucocorticoids-by-metabolite-recycling .

5. Navidate to the cloned repository directory, Run the conainer by mounting the ./src/ directory of the repo to /tmp/ in the container:

   • docker run -ti -v $(pwd)/src:/mnt Tumors-produce-glucocorticoids-by-metabolite-recycling

6. Run the following code.

   • cd /mnt
   • bash run_pipeline.sh