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nbase filter #23

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zhoudreames opened this issue Oct 27, 2023 · 2 comments
Open

nbase filter #23

zhoudreames opened this issue Oct 27, 2023 · 2 comments

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@zhoudreames
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zhoudreames commented Oct 27, 2023

image
i want to exclude N >1000 reads for RNA bam files, for example, the reads "9ae8a1cd-1a71-40d1-ab06-f1567ec79f9a" contained 1593N, it should be filtered. However , this software not work when I used this code "variant GW004.ONT.RNA.sorted.filter.chr7.bam --max-nbases 1000 -o GW004.ONT.RNA.sorted.filter.chr7.N10k.bam -b -t100"
This why? Thanks~

@zhoudreames
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by using the above code, the file size is same as the raw bam files, suggest this n-base filter is not work @chapmanb @llincoln3 @walaj I am so sorry to disturb you, can you help me?

@walaj
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walaj commented Oct 30, 2023

Thanks for finding this. I found the bug and just pushed changes. Will need to update the SeqLib submodule as well, easiest would be just re-clone (recursive) VariantBam and rebuild

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