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We are not identifying known SNPs near the 3' end of rRNA in Flavobacterium johnsoniae - we confirmed that the SNPs were present in every sample/relevant read by examining mapped reads. We assume that snippy doesn't identify SNPs here because the reads will be multimapped to all 16S rRNA copies in the genome - is there a flag or approach that allows multimapped reads to be carried through in SNP analysis?
Thank you for your efforts and tool!
The text was updated successfully, but these errors were encountered:
Hello,
We are not identifying known SNPs near the 3' end of rRNA in Flavobacterium johnsoniae - we confirmed that the SNPs were present in every sample/relevant read by examining mapped reads. We assume that snippy doesn't identify SNPs here because the reads will be multimapped to all 16S rRNA copies in the genome - is there a flag or approach that allows multimapped reads to be carried through in SNP analysis?
Thank you for your efforts and tool!
The text was updated successfully, but these errors were encountered: