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<REC r_id_disclaimer="ResearcherID data provided by Clarivate Analytics"><UID>WOS:000258627600009</UID><static_data><summary><EWUID><WUID coll_id="WOS"></WUID><edition value="WOS.SCI"></edition></EWUID><pub_info sortdate="2008-08-15" pubyear="2008" has_abstract="Y" coverdate="AUG 15 2008" pubmonth="AUG 15" vol="314" issue="14" pubtype="Journal"><page begin="2634" end="2642" page_count="9">2634-2642</page></pub_info><titles count="6"><title type="source">EXPERIMENTAL CELL RESEARCH</title><title type="source_abbrev">EXP CELL RES</title><title type="abbrev_iso">Exp. Cell Res.</title><title type="abbrev_11">EXP CELL RE</title><title type="abbrev_29">EXP CELL RES</title><title type="item">Rapid induction of pluripotency genes after exposure of human somatic cells to mouse ES cell extracts</title></titles><names count="6"><name seq_no="1" role="author" addr_no="1" daisng_id="3354458"><display_name>Bru, Thierry</display_name><full_name>Bru, Thierry</full_name><wos_standard>Bru, T</wos_standard><first_name>Thierry</first_name><last_name>Bru</last_name></name><name seq_no="2" role="author" addr_no="2 3" daisng_id="7451776"><display_name>Clarke, Catriona</display_name><full_name>Clarke, Catriona</full_name><wos_standard>Clarke, C</wos_standard><first_name>Catriona</first_name><last_name>Clarke</last_name></name><name seq_no="3" role="author" addr_no="3" daisng_id="1084769"><display_name>McGrew, Michael J.</display_name><full_name>McGrew, Michael J.</full_name><wos_standard>McGrew, MJ</wos_standard><first_name>Michael J.</first_name><last_name>McGrew</last_name></name><name seq_no="4" role="author" addr_no="3" daisng_id="622360"><display_name>Sang, Helen M.</display_name><full_name>Sang, Helen M.</full_name><wos_standard>Sang, HM</wos_standard><first_name>Helen M.</first_name><last_name>Sang</last_name></name><name seq_no="5" role="author" addr_no="2 3" daisng_id="61750"><display_name>Wilmut, Ian</display_name><full_name>Wilmut, Ian</full_name><wos_standard>Wilmut, I</wos_standard><first_name>Ian</first_name><last_name>Wilmut</last_name></name><name seq_no="6" role="author" reprint="Y" addr_no="1" daisng_id="205698"><display_name>Blow, J. Julian</display_name><full_name>Blow, J. Julian</full_name><wos_standard>Blow, JJ</wos_standard><first_name>J. Julian</first_name><last_name>Blow</last_name><email_addr>j.j.blow@dundee.ac.uk</email_addr></name></names><doctypes count="1"><doctype>Article</doctype></doctypes><publishers><publisher><address_spec addr_no="1"><full_address>525 B STREET, STE 1900, SAN DIEGO, CA 92101-4495 USA</full_address><city>SAN DIEGO</city></address_spec><names count="1"><name role="publisher" seq_no="1" addr_no="1"><display_name>ELSEVIER INC</display_name><full_name>ELSEVIER INC</full_name></name></names></publisher></publishers></summary><fullrecord_metadata><languages count="1"><language type="primary">English</language></languages><normalized_languages count="1"><language type="primary">English</language></normalized_languages><normalized_doctypes count="1"><doctype>Article</doctype></normalized_doctypes><refs count="31"></refs><addresses count="3"><address_name><address_spec addr_no="1"><full_address>Univ Dundee, Coll Life Sci, Dundee DD1 5EH, Scotland</full_address><organizations count="2"><organization>Univ Dundee</organization><organization pref="Y">University of Dundee</organization></organizations><suborganizations count="1"><suborganization>Coll Life Sci</suborganization></suborganizations><city>Dundee</city><country>Scotland</country><zip location="AC">DD1 5EH</zip></address_spec><names count="2"><name seq_no="1" role="author" addr_no="1" daisng_id="3354458"><display_name>Bru, Thierry</display_name><full_name>Bru, Thierry</full_name><wos_standard>Bru, T</wos_standard><first_name>Thierry</first_name><last_name>Bru</last_name></name><name seq_no="6" role="author" reprint="Y" addr_no="1" daisng_id="205698"><display_name>Blow, J. Julian</display_name><full_name>Blow, J. Julian</full_name><wos_standard>Blow, JJ</wos_standard><first_name>J. Julian</first_name><last_name>Blow</last_name><email_addr>j.j.blow@dundee.ac.uk</email_addr></name></names></address_name><address_name><address_spec addr_no="2"><full_address>Univ Edinburgh, Ctr Regenerat Med, Edinburgh EH8 9YL, Midlothian, Scotland</full_address><organizations count="2"><organization>Univ Edinburgh</organization><organization pref="Y">University of Edinburgh</organization></organizations><suborganizations count="1"><suborganization>Ctr Regenerat Med</suborganization></suborganizations><city>Edinburgh</city><state>Midlothian</state><country>Scotland</country><zip location="AC">EH8 9YL</zip></address_spec><names count="2"><name seq_no="2" role="author" addr_no="2" daisng_id="7451776"><display_name>Clarke, Catriona</display_name><full_name>Clarke, Catriona</full_name><wos_standard>Clarke, C</wos_standard><first_name>Catriona</first_name><last_name>Clarke</last_name></name><name seq_no="5" role="author" addr_no="2" daisng_id="61750"><display_name>Wilmut, Ian</display_name><full_name>Wilmut, Ian</full_name><wos_standard>Wilmut, I</wos_standard><first_name>Ian</first_name><last_name>Wilmut</last_name></name></names></address_name><address_name><address_spec addr_no="3"><full_address>Roslin Inst, Roslin EH25 9PS, Midlothian, Scotland</full_address><organizations count="2"><organization>Roslin Inst</organization><organization pref="Y">BBSRC Roslin Institute</organization></organizations><city>Roslin</city><state>Midlothian</state><country>Scotland</country><zip location="AC">EH25 9PS</zip></address_spec><names count="4"><name seq_no="2" role="author" addr_no="3" daisng_id="7451776"><display_name>Clarke, Catriona</display_name><full_name>Clarke, Catriona</full_name><wos_standard>Clarke, C</wos_standard><first_name>Catriona</first_name><last_name>Clarke</last_name></name><name seq_no="3" role="author" addr_no="3" daisng_id="1084769"><display_name>McGrew, Michael J.</display_name><full_name>McGrew, Michael J.</full_name><wos_standard>McGrew, MJ</wos_standard><first_name>Michael J.</first_name><last_name>McGrew</last_name></name><name seq_no="4" role="author" addr_no="3" daisng_id="622360"><display_name>Sang, Helen M.</display_name><full_name>Sang, Helen M.</full_name><wos_standard>Sang, HM</wos_standard><first_name>Helen M.</first_name><last_name>Sang</last_name></name><name seq_no="5" role="author" addr_no="3" daisng_id="61750"><display_name>Wilmut, Ian</display_name><full_name>Wilmut, Ian</full_name><wos_standard>Wilmut, I</wos_standard><first_name>Ian</first_name><last_name>Wilmut</last_name></name></names></address_name></addresses><reprint_addresses count="1"><address_name><address_spec addr_no="1"><full_address>Univ Dundee, Coll Life Sci, Dow St, Dundee DD1 5EH, Scotland</full_address><organizations count="2"><organization>Univ Dundee</organization><organization pref="Y">University of Dundee</organization></organizations><suborganizations count="1"><suborganization>Coll Life Sci</suborganization></suborganizations><street>Dow St</street><city>Dundee</city><country>Scotland</country><zip location="AC">DD1 5EH</zip></address_spec><names count="1"><name seq_no="1" role="author" reprint="Y" addr_no="1"><display_name>Blow, J. Julian</display_name><full_name>Blow, J. Julian</full_name><wos_standard>Blow, JJ</wos_standard><first_name>J. Julian</first_name><last_name>Blow</last_name><email_addr>j.j.blow@dundee.ac.uk</email_addr></name></names></address_name></reprint_addresses><category_info><headings count="1"><heading>Science &amp; Technology</heading></headings><subheadings count="1"><subheading>Life Sciences &amp; Biomedicine</subheading></subheadings><subjects count="4"><subject ascatype="traditional" code="DM">Oncology</subject><subject ascatype="traditional" code="DR">Cell Biology</subject><subject ascatype="extended">Oncology</subject><subject ascatype="extended">Cell Biology</subject></subjects></category_info><fund_ack><fund_text><p>Thanks to Neil Perkins for his comments on the manuscript. This work was supported by BBSRC grant G20421 (TB and CC) and CRUK grants C303/A3135 and C303/A7399 (JJB). The authors have no competing interests in the work presented.nere.</p></fund_text><grants count="4"><grant><grant_agency>BBSRC</grant_agency><grant_ids count="1"><grant_id>G20421</grant_id></grant_ids></grant><grant><grant_agency>CRUK</grant_agency><grant_ids count="2"><grant_id>C303/A3135</grant_id><grant_id>C303/A7399</grant_id></grant_ids></grant><grant grant_source="Researchfish"><grant_agency>Biotechnology and Biological Sciences Research Council</grant_agency><grant_ids count="1"><grant_id>G20421</grant_id></grant_ids></grant><grant grant_source="Researchfish"><grant_agency>Medical Research Council</grant_agency><grant_ids count="1"><grant_id>G0700711B</grant_id></grant_ids></grant></grants></fund_ack><keywords count="5"><keyword>reprogramming</keyword><keyword>pluripotent</keyword><keyword>embryonic stem cells</keyword><keyword>gene expression</keyword><keyword>oct-4 expression levels</keyword></keywords><abstracts count="1"><abstract><abstract_text count="1"><p>The expression of 4 pluripotency genes (Oct4, Sox2, c-Myc and Klf4) in mouse embryonic fibroblasts can reprogramme them to a pluripotent state. We have investigated the expression of these pluripotency genes when human somatic 293T cells are permeabilized and incubated in extracts of mouse embryonic stem (ES) cells. Expression of all 4 genes was induced over 1-8 h. Gene expression was associated with loss of repressive histone H3 modifications and increased recruitment of RNA polymerase 11 at the promoters. Lamin A/C, which is typically found only in differentiated cells, was also removed from the nuclei. When 293T cells were returned to culture after exposure to ES cell extract, the expression of the pluripotency genes continued to rise over the following 48 h of culture, suggesting that long-term reprogramming of gene expression had been induced. This provides a methodology for studying the de-differentiation of somatic cells that can potentially lead to an efficient way of reprograrnming somatic cells to a pluripotent state without genetically altering them. (C) 2008 Elsevier Inc. All rights reserved.</p></abstract_text></abstract></abstracts></fullrecord_metadata><item xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="itemType_wos" coll_id="WOS"><ids avail="N">340DZ</ids><bib_id>314 (14): 2634-2642 AUG 15 2008</bib_id><bib_pagecount type="Journal">208</bib_pagecount><keywords_plus count="10"><keyword>EMBRYONIC STEM-CELLS</keyword><keyword>IN-VITRO</keyword><keyword>DEFINED FACTORS</keyword><keyword>NUCLEI</keyword><keyword>FIBROBLASTS</keyword><keyword>EXPRESSION</keyword><keyword>CARCINOMA</keyword><keyword>NANOG</keyword><keyword>OCT4</keyword><keyword>DIFFERENTIATION</keyword></keywords_plus></item><contributors count="1"><contributor><name seq_no="1" role="researcher_id" r_id="B-3977-2009" orcid_id="0000-0002-9524-5849"><display_name>Blow, J. Julian</display_name><full_name>Blow, J. Julian</full_name><first_name>J. Julian</first_name><last_name>Blow</last_name></name></contributor></contributors></static_data><dynamic_data><citation_related><tc_list><silo_tc coll_id="WOS" local_count="56"></silo_tc></tc_list></citation_related><cluster_related><identifiers><identifier type="issn" value="0014-4827"></identifier><identifier type="eissn" value="1090-2422"></identifier><identifier type="doi" value="10.1016/j.yexcr.2008.05,009"></identifier><identifier type="xref_doi" value="10.1016/j.yexcr.2008.05.009"></identifier></identifiers></cluster_related></dynamic_data></REC>