EcoFunPrimer/Dr. Rosen's lab

[kaneja123]

~/RDPTools/PrimerDesign/dist$ ls
PrimerDesign.jar
RDPTools/PrimerDesign/dist$ pwd
~/RDPTools/PrimerDesign/dist$ cd
$ java -Xmx8g -jar /home/eesi/RDPTools/PrimerDesign/dist/PrimerDesign.jar --help
usage: java -jar PrimerDesign.jar [--help] (-i|--input)
Shows all the files
$ java -Xmx8g -jar /home/eesi/RDPTools/PrimerDesign/dist/PrimerDesign.jar -subcommand screen -input Desktop/62518burkholderiales_coae_2_extended.fa -GraphOutput Desktop/ -oligoMinSize 15 -oligoMaxSize 30 -tempMin 55 -tempMax 63 -hairMax 35 -homoMax 35 -os mac -NoTEndFilter t -NoPoly3GCFilter t -PolyRunFilter 4 -GCFilterMin 0.45 -GCFi
python: can't open file '/home/eesi/RDPTools/PrimerDesign/dist/primer3/primersplot.py': [Errno 2] No such file or directory
End compile data points: 25/06/18 15:24:41

primersplot.py': [Errno 2] No such file or directory
Where is this file? Why it cannot locate it?

[mdehollander]

@rdpstaffmsu @gunturus
I have the same issue that primersplot.py is not present in the dist directory. Is this script part of primer3 or should this be added to your repository?