From d783ef9deb98dbd4adfabb5ab0858ba60a658057 Mon Sep 17 00:00:00 2001
From: Christian Kubica <chriskub@CK-MBP14USIctx.local>
Date: Tue, 10 May 2022 14:35:54 +0200
Subject: [PATCH 001/227] REVERT CHANGES

Reverting changes to a non-linted version and added the umitools modules.
---
 modules.json                                  |  8 ++-
 .../nf-core/modules/umitools/dedup/main.nf    | 41 +++++++++++++
 .../nf-core/modules/umitools/dedup/meta.yml   | 59 +++++++++++++++++++
 .../nf-core/modules/umitools/extract/main.nf  | 55 +++++++++++++++++
 .../nf-core/modules/umitools/extract/meta.yml | 47 +++++++++++++++
 5 files changed, 209 insertions(+), 1 deletion(-)
 create mode 100644 modules/nf-core/modules/umitools/dedup/main.nf
 create mode 100644 modules/nf-core/modules/umitools/dedup/meta.yml
 create mode 100644 modules/nf-core/modules/umitools/extract/main.nf
 create mode 100644 modules/nf-core/modules/umitools/extract/meta.yml

diff --git a/modules.json b/modules.json
index 81208b16..071935f8 100644
--- a/modules.json
+++ b/modules.json
@@ -32,7 +32,13 @@
             },
             "trimgalore": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+            },
+            "umitools/dedup": {
+                "git_sha": "f425aa3cea10015fe9b345b9d6dcc2336b53155f"
+            },
+            "umitools/extract": {
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/umitools/dedup/main.nf b/modules/nf-core/modules/umitools/dedup/main.nf
new file mode 100644
index 00000000..dfcbcf2f
--- /dev/null
+++ b/modules/nf-core/modules/umitools/dedup/main.nf
@@ -0,0 +1,41 @@
+process UMITOOLS_DEDUP {
+    tag "$meta.id"
+    label "process_medium"
+
+    conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
+        'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+
+    output:
+    tuple val(meta), path("*.bam")             , emit: bam
+    tuple val(meta), path("*edit_distance.tsv"), emit: tsv_edit_distance
+    tuple val(meta), path("*per_umi.tsv")      , emit: tsv_per_umi
+    tuple val(meta), path("*per_position.tsv") , emit: tsv_umi_per_position
+    path  "versions.yml"                       , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def paired = meta.single_end ? "" : "--paired"
+    """
+    umi_tools \\
+        dedup \\
+        -I $bam \\
+        -S ${prefix}.bam \\
+        --output-stats $prefix \\
+        $paired \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/umitools/dedup/meta.yml b/modules/nf-core/modules/umitools/dedup/meta.yml
new file mode 100644
index 00000000..eee8952f
--- /dev/null
+++ b/modules/nf-core/modules/umitools/dedup/meta.yml
@@ -0,0 +1,59 @@
+name: umitools_dedup
+description: Deduplicate reads based on the mapping co-ordinate and the UMI attached to the read.
+keywords:
+  - umitools
+  - deduplication
+tools:
+  - umi_tools:
+    description: >
+      UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs)
+      and single cell RNA-Seq cell barcodes
+    documentation: https://umi-tools.readthedocs.io/en/latest/
+    license: ["MIT"]
+input:
+  - meta:
+    type: map
+    description: |
+      Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+    type: file
+    description: |
+      BAM file containing reads to be deduplicated via UMIs.
+    pattern: "*.{bam}"
+  - bai:
+    type: file
+    description: |
+      BAM index files corresponding to the input BAM file.
+    pattern: "*.{bai}"
+output:
+  - meta:
+    type: map
+    description: |
+      Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+    type: file
+    description: BAM file with deduplicated UMIs.
+    pattern: "*.{bam}"
+  - tsv_edit_distance:
+    type: file
+    description: Reports the (binned) average edit distance between the UMIs at each position.
+    pattern: "*edit_distance.tsv"
+  - tsv_per_umi:
+    type: file
+    description: UMI-level summary statistics.
+    pattern: "*per_umi.tsv"
+  - tsv_umi_per_position:
+    type: file
+    description: Tabulates the counts for unique combinations of UMI and position.
+    pattern: "*per_position.tsv"
+  - versions:
+    type: file
+    description: File containing software versions
+    pattern: "versions.yml"
+
+authors:
+  - "@drpatelh"
+  - "@grst"
+  - "@klkeys"
diff --git a/modules/nf-core/modules/umitools/extract/main.nf b/modules/nf-core/modules/umitools/extract/main.nf
new file mode 100644
index 00000000..22a405b9
--- /dev/null
+++ b/modules/nf-core/modules/umitools/extract/main.nf
@@ -0,0 +1,55 @@
+process UMITOOLS_EXTRACT {
+    tag "$meta.id"
+    label "process_low"
+
+    conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
+        'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+
+    output:
+    tuple val(meta), path("*.fastq.gz"), emit: reads
+    tuple val(meta), path("*.log")     , emit: log
+    path  "versions.yml"               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    if (meta.single_end) {
+        """
+        umi_tools \\
+            extract \\
+            -I $reads \\
+            -S ${prefix}.umi_extract.fastq.gz \\
+            $args \\
+            > ${prefix}.umi_extract.log
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
+        END_VERSIONS
+        """
+    }  else {
+        """
+        umi_tools \\
+            extract \\
+            -I ${reads[0]} \\
+            --read2-in=${reads[1]} \\
+            -S ${prefix}.umi_extract_1.fastq.gz \\
+            --read2-out=${prefix}.umi_extract_2.fastq.gz \\
+            $args \\
+            > ${prefix}.umi_extract.log
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
+        END_VERSIONS
+        """
+    }
+}
diff --git a/modules/nf-core/modules/umitools/extract/meta.yml b/modules/nf-core/modules/umitools/extract/meta.yml
new file mode 100644
index 00000000..7fc23f72
--- /dev/null
+++ b/modules/nf-core/modules/umitools/extract/meta.yml
@@ -0,0 +1,47 @@
+name: umitools_extract
+description: Extracts UMI barcode from a read and add it to the read name, leaving any sample barcode in place
+keywords:
+  - umitools
+  - extract
+tools:
+  - umi_tools:
+    description: >
+      UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs)
+      and single cell RNA-Seq cell barcodes
+    documentation: https://umi-tools.readthedocs.io/en/latest/
+    license: ["MIT"]
+input:
+  - meta:
+    type: map
+    description: |
+      Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+    type: list
+    description: |
+      List of input FASTQ files whose UMIs will be extracted.
+output:
+  - meta:
+    type: map
+    description: |
+      Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+    type: file
+    description: >
+      Extracted FASTQ files. |
+      For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. |
+        For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
+    pattern: "*.{fastq.gz}"
+  - log:
+    type: file
+    description: Logfile for umi_tools
+    pattern: "*.{log}"
+  - versions:
+    type: file
+    description: File containing software versions
+    pattern: "versions.yml"
+
+authors:
+  - "@drpatelh"
+  - "@grst"

From 1043932a309d7ce573973225f16e62e4f7f82e3e Mon Sep 17 00:00:00 2001
From: Christian Kubica <chriskub@CK-MBP14USIctx.local>
Date: Tue, 10 May 2022 15:58:50 +0200
Subject: [PATCH 002/227] INCLUDE UMITOOLS WORKFLOW

Added the umitools workflow and integrated it into the smrnaseq workflow
---
 .../nf-core/fastqc_umitools_trimgalore.nf     | 78 +++++++++++++++++++
 workflows/smrnaseq.nf                         | 14 ++--
 2 files changed, 86 insertions(+), 6 deletions(-)
 create mode 100644 subworkflows/nf-core/fastqc_umitools_trimgalore.nf

diff --git a/subworkflows/nf-core/fastqc_umitools_trimgalore.nf b/subworkflows/nf-core/fastqc_umitools_trimgalore.nf
new file mode 100644
index 00000000..ca158e7a
--- /dev/null
+++ b/subworkflows/nf-core/fastqc_umitools_trimgalore.nf
@@ -0,0 +1,78 @@
+//
+// Read QC, UMI extraction and trimming
+//
+
+nextflow.enable.dsl=2
+
+include { FASTQC           } from '../../modules/nf-core/modules/fastqc/main'
+include { UMITOOLS_EXTRACT } from '../../modules/nf-core/modules/umitools/extract/main'
+include { TRIMGALORE       } from '../../modules/nf-core/modules/trimgalore/main'
+
+workflow FASTQC_UMITOOLS_TRIMGALORE {
+    take:
+    reads            // channel: [ val(meta), [ reads ] ]
+    skip_fastqc      // boolean: true/false
+    with_umi         // boolean: true/false
+    skip_trimming    // boolean: true/false
+    umi_discard_read // integer: 0, 1 or 2
+
+    main:
+
+    ch_versions = Channel.empty()
+    fastqc_html = Channel.empty()
+    fastqc_zip  = Channel.empty()
+    if (!skip_fastqc) {
+        FASTQC ( reads ).html.set { fastqc_html }
+        fastqc_zip  = FASTQC.out.zip
+        ch_versions = ch_versions.mix(FASTQC.out.versions.first())
+    }
+
+    umi_reads = reads
+    umi_log   = Channel.empty()
+    if (with_umi) {
+        UMITOOLS_EXTRACT ( reads ).reads.set { umi_reads }
+        umi_log     = UMITOOLS_EXTRACT.out.log
+        ch_versions = ch_versions.mix(UMITOOLS_EXTRACT.out.versions.first())
+
+        // Discard R1 / R2 if required
+        if (umi_discard_read in [1,2]) {
+            UMITOOLS_EXTRACT
+                .out
+                .reads
+                .map { meta, reads ->
+                    if (!meta.single_end) {
+                        meta['single_end'] = true
+                        reads = reads[umi_discard_read % 2]
+                    }
+                    return [ meta, reads ]
+                }
+                .set { umi_reads }
+        }
+    }
+
+    trim_reads = umi_reads
+    trim_html  = Channel.empty()
+    trim_zip   = Channel.empty()
+    trim_log   = Channel.empty()
+    if (!skip_trimming) {
+        TRIMGALORE ( umi_reads ).reads.set { trim_reads }
+        trim_html   = TRIMGALORE.out.html
+        trim_zip    = TRIMGALORE.out.zip
+        trim_log    = TRIMGALORE.out.log
+        ch_versions = ch_versions.mix(TRIMGALORE.out.versions.first())
+    }
+
+    emit:
+    reads = trim_reads // channel: [ val(meta), [ reads ] ]
+
+    fastqc_html        // channel: [ val(meta), [ html ] ]
+    fastqc_zip         // channel: [ val(meta), [ zip ] ]
+
+    umi_log            // channel: [ val(meta), [ log ] ]
+
+    trim_html          // channel: [ val(meta), [ html ] ]
+    trim_zip           // channel: [ val(meta), [ zip ] ]
+    trim_log           // channel: [ val(meta), [ txt ] ]
+
+    versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ]
+}
\ No newline at end of file
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index ff8669bf..1613425c 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -55,7 +55,7 @@ if (params.mature) { reference_mature = file(params.mature, checkIfExists: true)
 if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin miRNA fasta file not found: ${params.hairpin}" }
 
 include { INPUT_CHECK       } from '../subworkflows/local/input_check'
-include { FASTQC_TRIMGALORE } from '../subworkflows/nf-core/fastqc_trimgalore'
+include { FASTQC_UMITOOLS_TRIMGALORE } from '../subworkflows/nf-core/fastqc_umitools_trimgalore'
 include { MIRNA_QUANT       } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT      } from '../subworkflows/local/genome_quant'
 include { MIRTRACE          } from '../subworkflows/local/mirtrace'
@@ -133,14 +133,16 @@ workflow SMRNASEQ {
     //
     // SUBWORKFLOW: Read QC, extract UMI and trim adapters
     //
-    FASTQC_TRIMGALORE (
+    FASTQC_UMITOOLS_TRIMGALORE (
         ch_cat_fastq,
+        params.with_umi,
+        params.umi_discard_read,
         params.skip_fastqc || params.skip_qc,
         params.skip_trimming
     )
-    ch_versions = ch_versions.mix(FASTQC_TRIMGALORE.out.versions)
+    ch_versions = ch_versions.mix(FASTQC_UMITOOLS_TRIMGALORE.out.versions)
 
-    reads_for_mirna = FASTQC_TRIMGALORE.out.reads
+    reads_for_mirna = FASTQC_UMITOOLS_TRIMGALORE.out.reads
     MIRNA_QUANT (
         reference_mature,
         reference_hairpin,
@@ -161,7 +163,7 @@ workflow SMRNASEQ {
         ch_versions = ch_versions.mix(GENOME_QUANT.out.versions)
 
         if (!params.skip_mirdeep) {
-            MIRDEEP2 (FASTQC_TRIMGALORE.out.reads, GENOME_QUANT.out.fasta, GENOME_QUANT.out.indices, MIRNA_QUANT.out.fasta_hairpin, MIRNA_QUANT.out.fasta_mature)
+            MIRDEEP2 (FASTQC_UMITOOLS_TRIMGALORE.out.reads, GENOME_QUANT.out.fasta, GENOME_QUANT.out.indices, MIRNA_QUANT.out.fasta_hairpin, MIRNA_QUANT.out.fasta_mature)
             ch_versions = ch_versions.mix(MIRDEEP2.out.versions)
         }
     }
@@ -186,7 +188,7 @@ workflow SMRNASEQ {
         ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
 
-        ch_multiqc_files = ch_multiqc_files.mix(FASTQC_TRIMGALORE.out.fastqc_zip.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQC_UMITOOLS_TRIMGALORE.out.fastqc_zip.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mature_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.hairpin_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(genome_stats.collect({it[1]}).ifEmpty([]))

From 27fd482b7eba23b47b8526a1eb32bee489bc3d53 Mon Sep 17 00:00:00 2001
From: Christian Kubica <chriskub@CK-MBP14USIctx.local>
Date: Fri, 13 May 2022 13:03:20 +0200
Subject: [PATCH 003/227] ADD DOCUMENTATION

Add additional documentation to use UMI tools as part of the pipeline.
Most of the documentation has been copied from nf-core/rnaseq.
---
 CHANGELOG.md         | 16 +++++++++++-----
 README.md            | 17 +++++++++--------
 docs/output.md       | 16 ++++++++++++++++
 nextflow.config      |  8 ++++++++
 nextflow_schema.json | 45 ++++++++++++++++++++++++++++++++++++++++++++
 5 files changed, 89 insertions(+), 13 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e1b6875e..db1eec43 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,14 +21,20 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### Other enhancements & fixes
 
 - [#134](https://github.com/nf-core/smrnaseq/issues/134) - Fixed colSum of zero issues for edgeR_miRBase.R script
+- [#49](https://github.com/nf-core/smrnaseq/issues/49) - Integrated the existing umitools modules into the pipeline.
 
 ### Parameters
 
-| Old parameter        | New parameter    |
-| -------------------- | ---------------- |
-| `--conda`            | `--enable_conda` |
-| `--clusterOptions`   |                  |
-| `--publish_dir_mode` |                  |
+| Old parameter        | New parameter               |
+| -------------------- | --------------------------- |
+| `--conda`            | `--enable_conda`            |
+| `--clusterOptions`   |                             |
+| `--publish_dir_mode` |                             |
+|                      | `--with_umi`                |
+|                      | `--umitools_extract_method` |
+|                      | `--umitools_bc_pattern`     |
+|                      | `--umi_discard_read`        |
+|                      | `--save_umi_intermeds`      |
 
 > **NB:** Parameter has been **updated** if both old and new parameter information is present.
 > **NB:** Parameter has been **added** if just the new parameter information is present.
diff --git a/README.md b/README.md
index 6d3efa52..e4294f99 100644
--- a/README.md
+++ b/README.md
@@ -31,27 +31,28 @@ On release, automated continuous integration tests run the pipeline on a full-si
 ## Pipeline summary
 
 1. Raw read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-2. Adapter trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/))
+2. UMI barcode extraction ([`UMI-tools`](https://github.com/CGATOxford/UMI-tools))
+3. Adapter trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/))
    1. Insert Size calculation
    2. Collapse reads ([`seqcluster`](https://seqcluster.readthedocs.io/mirna_annotation.html#processing-of-reads))
-3. Alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-4. Alignment against miRBase hairpin
+4. Alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
+5. Alignment against miRBase hairpin
    1. Unaligned reads from step 3 ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
    2. Collapsed reads from step 2.2 ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-5. Post-alignment processing of miRBase hairpin
+6. Post-alignment processing of miRBase hairpin
    1. Basic statistics from step 3 and step 4.1 ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
    2. Analysis on miRBase hairpin counts ([`edgeR`](https://bioconductor.org/packages/release/bioc/html/edgeR.html))
       - TMM normalization and a table of top expression hairpin
       - MDS plot clustering samples
       - Heatmap of sample similarities
    3. miRNA and isomiR annotation from step 4.1 ([`mirtop`](https://github.com/miRTop/mirtop))
-6. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
+7. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
    1. Post-alignment processing of alignment against host reference genome ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
-7. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation))
+8. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation))
    1. Mapping against reference genome with the mapper module
    2. Known and novel miRNA discovery with the mirdeep2 module
-8. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace))
-9. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
+9. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace))
+10. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
 
 ## Quick Start
 
diff --git a/docs/output.md b/docs/output.md
index ce1f8347..79f1d6d9 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -13,6 +13,7 @@ The directories listed below will be created in the results directory after the
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
 - [FastQC](#fastqc) - read quality control
+- [UMI-tools extract](#umi-tools-extract) - UMI barcode extraction
 - [TrimGalore](#trimgalore) - adapter trimming
 - [Bowtie](#bowtie) - alignment against mature miRNAs and miRNA precursors (hairpins)
 - [SAMtools](#samtools) - alignment result processing and feature counting
@@ -39,6 +40,21 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 ![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png)
 
+## UMI-tools extract
+
+<details markdown="1">
+<summary>Output files</summary>
+
+- `umitools/`
+  - `*.fastq.gz`: If `--save_umi_intermeds` is specified, FastQ files **after** UMI extraction will be placed in this directory.
+  - `*.log`: Log file generated by the UMI-tools `extract` command.
+
+</details>
+
+[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name. Secondly, reads are deduplicated based on UMI identifier after mapping as highlighted in the [UMI-tools dedup](#umi-tools-dedup) section.
+
+To facilitate processing of input data which has the UMI barcode already embedded in the read name from the start, `--skip_umi_extract` can be specified in conjunction with `--with_umi`.
+
 ## TrimGalore
 
 [TrimGalore](http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/) is used for removal of adapter contamination and trimming of low quality regions. TrimGalore uses [Cutadapt](https://github.com/marcelm/cutadapt) for adapter trimming and runs FastQC after it finishes.
diff --git a/nextflow.config b/nextflow.config
index c5fa807d..4d724120 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -27,6 +27,14 @@ params {
     mature                     = "https://mirbase.org/ftp/CURRENT/mature.fa.gz"
     hairpin                    = "https://mirbase.org/ftp/CURRENT/hairpin.fa.gz"
 
+    // UMI handling
+    with_umi                   = false
+    skip_umi_extract           = false
+    umitools_extract_method    = 'string'
+    umitools_bc_pattern        = null
+    umi_discard_read           = null
+    save_umi_intermeds         = false
+
     // Trimming options
     clip_r1                    = 0
     three_prime_clip_r1        = 0
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 027f1b37..5019408f 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -50,6 +50,48 @@
                 }
             }
         },
+        "umi_options": {
+            "title": "UMI options",
+            "type": "object",
+            "description": "Options for processing reads with unique molecular identifiers",
+            "default": "",
+            "properties": {
+                "with_umi": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-barcode",
+                    "description": "Enable UMI-based read deduplication."
+                },
+                "umitools_extract_method": {
+                    "type": "string",
+                    "default": "string",
+                    "fa_icon": "fas fa-barcode",
+                    "description": "UMI pattern to use. Can be either 'string' (default) or 'regex'.",
+                    "help_text": "More details can be found in the [UMI-tools documentation](https://umi-tools.readthedocs.io/en/latest/reference/extract.html#extract-method).\n"
+                },
+                "skip_umi_extract": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-compress-alt",
+                    "description": "Skip the UMI extraction from the read in case the UMIs have been moved to the headers in advance of the pipeline run."
+                },
+                "umitools_bc_pattern": {
+                    "type": "string",
+                    "fa_icon": "fas fa-barcode",
+                    "help_text": "More details can be found in the [UMI-tools documentation](https://umi-tools.readthedocs.io/en/latest/reference/extract.html#extract-method).",
+                    "description": "The UMI barcode pattern to use e.g. 'NNNNNN' indicates that the first 6 nucleotides of the read are from the UMI."
+                },
+                "umi_discard_read": {
+                    "type": "integer",
+                    "fa_icon": "fas fa-barcode",
+                    "description": "After UMI barcode extraction discard either R1 or R2 by setting this parameter to 1 or 2, respectively."
+                },
+                "save_umi_intermeds": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-save",
+                    "description": "If this option is specified, intermediate FastQ and BAM files produced by UMI-tools are also saved in the results directory."
+                }
+            },
+            "fa_icon": "fas fa-barcode"
+        },
         "reference_genome_options": {
             "title": "Reference genome options",
             "type": "object",
@@ -379,6 +421,9 @@
         {
             "$ref": "#/definitions/input_output_options"
         },
+        {
+            "$ref": "#/definitions/umi_options"
+        },
         {
             "$ref": "#/definitions/reference_genome_options"
         },

From ee673b095f667b119bf1102e370b253a37ce8d4a Mon Sep 17 00:00:00 2001
From: Christian Kubica <chriskub@CK-MBP14USIctx.local>
Date: Mon, 13 Jun 2022 13:22:37 +0200
Subject: [PATCH 004/227] ADD SAMTOOLS BAM2FQ MODULE

The bam2fq module is neccessary to convert the deduplicated bam
files back into a fastq format to be fed into the existing
pipeline.
---
 modules.json                                  |  5 +-
 .../nf-core/modules/samtools/bam2fq/main.nf   | 56 +++++++++++++++++++
 .../nf-core/modules/samtools/bam2fq/meta.yml  | 55 ++++++++++++++++++
 3 files changed, 115 insertions(+), 1 deletion(-)
 create mode 100644 modules/nf-core/modules/samtools/bam2fq/main.nf
 create mode 100644 modules/nf-core/modules/samtools/bam2fq/meta.yml

diff --git a/modules.json b/modules.json
index 071935f8..c328879e 100644
--- a/modules.json
+++ b/modules.json
@@ -15,6 +15,9 @@
             "multiqc": {
                 "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
             },
+            "samtools/bam2fq": {
+                "git_sha": "5510ea39fe638594bc26ac34cadf4a84bf27d159"
+            },
             "samtools/flagstat": {
                 "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
             },
@@ -41,4 +44,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/modules/samtools/bam2fq/main.nf b/modules/nf-core/modules/samtools/bam2fq/main.nf
new file mode 100644
index 00000000..9301d1d3
--- /dev/null
+++ b/modules/nf-core/modules/samtools/bam2fq/main.nf
@@ -0,0 +1,56 @@
+process SAMTOOLS_BAM2FQ {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
+        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
+
+    input:
+    tuple val(meta), path(inputbam)
+    val split
+
+    output:
+    tuple val(meta), path("*.fq.gz"), emit: reads
+    path "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    if (split){
+        """
+        samtools \\
+            bam2fq \\
+            $args \\
+            -@ $task.cpus \\
+            -1 ${prefix}_1.fq.gz \\
+            -2 ${prefix}_2.fq.gz \\
+            -0 ${prefix}_other.fq.gz \\
+            -s ${prefix}_singleton.fq.gz \\
+            $inputbam
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+        END_VERSIONS
+        """
+    } else {
+        """
+        samtools \\
+            bam2fq \\
+            $args \\
+            -@ $task.cpus \\
+            $inputbam | gzip --no-name > ${prefix}_interleaved.fq.gz
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+        END_VERSIONS
+        """
+    }
+}
diff --git a/modules/nf-core/modules/samtools/bam2fq/meta.yml b/modules/nf-core/modules/samtools/bam2fq/meta.yml
new file mode 100644
index 00000000..319a60cf
--- /dev/null
+++ b/modules/nf-core/modules/samtools/bam2fq/meta.yml
@@ -0,0 +1,55 @@
+name: samtools_bam2fq
+description: |
+  The module uses bam2fq method from samtools to
+  convert a SAM, BAM or CRAM file to FASTQ format
+keywords:
+  - bam2fq
+  - samtools
+  - fastq
+tools:
+  - samtools:
+      description: Tools for dealing with SAM, BAM and CRAM files
+      homepage: None
+      documentation: http://www.htslib.org/doc/1.1/samtools.html
+      tool_dev_url: None
+      doi: ""
+      licence: ["MIT"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - inputbam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - split:
+      type: boolean
+      description: |
+        TRUE/FALSE value to indicate if reads should be separated into
+        /1, /2 and if present other, or singleton.
+        Note: choosing TRUE will generate 4 different files.
+        Choosing FALSE will produce a single file, which will be interleaved in case
+        the input contains paired reads.
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - reads:
+      type: file
+      description: |
+        FASTQ files, which will be either a group of 4 files (read_1, read_2, other and singleton)
+        or a single interleaved .fq.gz file if the user chooses not to split the reads.
+      pattern: "*.fq.gz"
+
+authors:
+  - "@lescai"

From 0bc65e456596ba8dca61b62616b351dc04c1af97 Mon Sep 17 00:00:00 2001
From: Christian Kubica <chriskub@CK-MBP14USIctx.local>
Date: Tue, 14 Jun 2022 14:13:23 +0200
Subject: [PATCH 005/227] ADD UMITOOLS EXTRACT ARGS

Added the umitools extract modules.config lines from nf-core/rnaseq
to this pipeline.
---
 conf/modules.config | 28 ++++++++++++++++++++++++++--
 1 file changed, 26 insertions(+), 2 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index c802ccf3..92fadab2 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -38,6 +38,30 @@ process {
     }
 }
 
+if (params.with_umi && !params.skip_umi_extract) {
+    process {
+        withName: '.*:FASTQC_UMITOOLS_TRIMGALORE:UMITOOLS_EXTRACT' {
+            ext.args   = [
+                    params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
+                    params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
+                ].join(' ').trim()
+            publishDir = [
+                [
+                    path: { "${params.outdir}/umitools" },
+                    mode: params.publish_dir_mode,
+                    pattern: "*.log"
+                ],
+                [
+                    path: { "${params.outdir}/umitools" },
+                    mode: params.publish_dir_mode,
+                    pattern: "*.fastq.gz",
+                    enabled: params.save_umi_intermeds
+                ]
+            ]
+        }
+    }
+}
+
 //
 // Genome preparation options
 //
@@ -89,7 +113,7 @@ process {
 
 if (!(params.skip_fastqc || params.skip_qc)) {
     process {
-        withName: '.*:FASTQC_TRIMGALORE:FASTQC' {
+        withName: '.*:FASTQC_UMITOOLS_TRIMGALORE:FASTQC' {
             ext.args = '--quiet'
         }
     }
@@ -97,7 +121,7 @@ if (!(params.skip_fastqc || params.skip_qc)) {
 
 if (!params.skip_trimming) {
     process {
-        withName: '.*:FASTQC_TRIMGALORE:TRIMGALORE' {
+        withName: '.*:FASTQC_UMITOOLS_TRIMGALORE:TRIMGALORE' {
             ext.args   = '--fastqc'
             publishDir = [
                 [

From 8d14f90a58b51460dfb41a47a7487ecbc272d40e Mon Sep 17 00:00:00 2001
From: Christian Kubica <chriskub@CK-MBP14USIctx.local>
Date: Wed, 15 Jun 2022 09:41:18 +0200
Subject: [PATCH 006/227] UPDATE MODULES.CONFIG

Added configurations for umi deduplication.
---
 conf/modules.config | 97 ++++++++++++++++++++++++++++++++++-----------
 1 file changed, 73 insertions(+), 24 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 92fadab2..c1a74e21 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -38,30 +38,6 @@ process {
     }
 }
 
-if (params.with_umi && !params.skip_umi_extract) {
-    process {
-        withName: '.*:FASTQC_UMITOOLS_TRIMGALORE:UMITOOLS_EXTRACT' {
-            ext.args   = [
-                    params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
-                    params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
-                ].join(' ').trim()
-            publishDir = [
-                [
-                    path: { "${params.outdir}/umitools" },
-                    mode: params.publish_dir_mode,
-                    pattern: "*.log"
-                ],
-                [
-                    path: { "${params.outdir}/umitools" },
-                    mode: params.publish_dir_mode,
-                    pattern: "*.fastq.gz",
-                    enabled: params.save_umi_intermeds
-                ]
-            ]
-        }
-    }
-}
-
 //
 // Genome preparation options
 //
@@ -101,6 +77,7 @@ process {
 //
 // Read QC and trimming options
 //
+
 process {
     withName: 'MIRTRACE_RUN' {
         publishDir = [
@@ -145,6 +122,78 @@ if (!params.skip_trimming) {
     }
 }
 
+if (params.with_umi && !params.skip_umi_extract) {
+    process {
+        withName: '.*:FASTQC_UMITOOLS_TRIMGALORE:UMITOOLS_EXTRACT' {
+            ext.args   = [
+                    params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
+                    params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
+                ].join(' ').trim()
+            publishDir = [
+                [
+                    path: { "${params.outdir}/umitools" },
+                    mode: params.publish_dir_mode,
+                    pattern: "*.log"
+                ],
+                [
+                    path: { "${params.outdir}/umitools" },
+                    mode: params.publish_dir_mode,
+                    pattern: "*.fastq.gz",
+                    enabled: params.save_umi_intermeds
+                ]
+            ]
+        }
+    }
+}
+
+//
+// UMI tools deduplication
+//
+
+if (params.with_umi) {
+    process {
+        withName: '.*:DEDUPLICATE_UMIS:UMITOOLS_DEDUP' {
+            ext.args = { meta.single_end ? '' : '--unpaired-reads=discard --chimeric-pairs=discard' }
+            ext.prefix = { "${meta.id}.umi_dedup.sorted" }
+            publishDir = [
+                [
+                    path: { "${params.outdir}/umi_dedup/umitools" },
+                    mode: params.publish_dir_mode,
+                    pattern: '*.tsv'
+                ],
+                [
+                    path: { "${params.outdir}/umi_dedup" },
+                    mode: params.publish_dir_mode,
+                    pattern: '*.bam',
+                    enabled: (
+                        params.save_umi_intermeds
+                    )
+                ]
+            ]
+        }
+
+        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX' {
+            ext.prefix = { "${meta.id}.umi_dedup.sorted" }
+            publishDir = [
+                path: { "${params.outdir}/umi_dedup" },
+                mode: params.publish_dir_mode,
+                pattern: '*.{bai,csi}',
+                enabled: (
+                    params.save_umi_intermeds
+                )
+            ]
+        }
+
+        withName: '.*:DEDUPLICATE_UMIS:BAM_STATS_SAMTOOLS:.*' {
+            publishDir = [
+                path: { "${params.outdir}/umi_dedup/samtools_stats" },
+                mode: params.publish_dir_mode,
+                pattern: '*.{stats,flagstat,idxstats}'
+            ]
+        }
+    }
+}
+
 //
 // Quantification
 //

From 23f96d83ea5bfcf40810d58797dc8f3b060a0303 Mon Sep 17 00:00:00 2001
From: Christian Kubica <chriskub@CK-MBP14USIctx.local>
Date: Wed, 15 Jun 2022 09:43:43 +0200
Subject: [PATCH 007/227] INCLUDE UMITOOLS DEDUP WORKFLOW

Initial comit of the umi dedup subworkflow. The workflow combines
already existing modules of the pipeline and nf-core module to
deduplicate the reads by mapping them to the species genome and
re-converting them to fastq after deduplication.
---
 subworkflows/local/umi_dedup.nf | 69 +++++++++++++++++++++++++++++++++
 1 file changed, 69 insertions(+)
 create mode 100644 subworkflows/local/umi_dedup.nf

diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
new file mode 100644
index 00000000..f97b8e8c
--- /dev/null
+++ b/subworkflows/local/umi_dedup.nf
@@ -0,0 +1,69 @@
+// 
+// Deduplicate the UMI reads by mapping them to the complete genome.
+//
+
+include { INDEX_GENOME                        } from '../../modules/local/bowtie_genome'
+include { BOWTIE_MAP_SEQ as BOWTIE_MAP_GENOME } from '../../modules/local/bowtie_map_mirna'
+include { BAM_SORT_SAMTOOLS                   } from '../../subworkflows/nf-core/bam_sort_samtools'
+include { UMITOOLS_DEDUP                      } from '../../modules/nf-core/modules/umitools/dedup/main'
+include { SAMTOOLS_BAM2FQ                     } from '../../modules/nf-core/modules/samtools/bam2fq/main'
+
+workflow DEDUPLICATE_UMIS {
+    take:
+    fasta
+    bt_index
+    reads      // channel: [ val(meta), [ reads ] ]
+
+    main:
+
+    ch_versions = Channel.empty()
+    ch_dedup_stats = Channel.empty()
+
+    if (!bt_index){
+        INDEX_GENOME ( fasta )
+        bt_indices      = INDEX_GENOME.out.bt_indices
+        fasta_formatted = INDEX_GENOME.out.fasta
+        ch_versions     = ch_versions.mix(INDEX_GENOME.out.versions)
+    } else {
+        bt_indices      = Channel.fromPath("${bt_index}**ebwt", checkIfExists: true).ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_index}" }
+        fasta_formatted = fasta
+    }
+
+    if (bt_indices){
+
+        reads.view()
+        
+        BOWTIE_MAP_GENOME ( reads, bt_indices.collect() )
+        ch_versions = ch_versions.mix(BOWTIE_MAP_GENOME.out.versions)
+
+        BAM_SORT_SAMTOOLS ( BOWTIE_MAP_GENOME.out.bam, Channel.empty() )
+        ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions)
+
+        BAM_SORT_SAMTOOLS.out.bam.view()
+        ch_umi_dedup = BAM_SORT_SAMTOOLS.out.bam.join(BAM_SORT_SAMTOOLS.out.bai)
+
+        ch_umi_dedup.view()
+
+        UMITOOLS_DEDUP ( ch_umi_dedup )
+        ch_versions = ch_versions.mix(UMITOOLS_DEDUP.out.versions)
+        ch_dedup_stats = ch_dedup_stats.mix(UMITOOLS_DEDUP.out.tsv_edit_distance).join(UMITOOLS_DEDUP.out.tsv_per_umi).join(UMITOOLS_DEDUP.out.tsv_umi_per_position)
+
+        SAMTOOLS_BAM2FQ ( UMITOOLS_DEDUP.out.bam, false )
+        ch_versions = ch_versions.mix(SAMTOOLS_BAM2FQ.out.versions)
+    }
+
+    emit:
+    reads    = SAMTOOLS_BAM2FQ.out.reads // channel: [ val(meta), [ reads ] ]
+    indices  = bt_indices
+    stats    = ch_dedup_stats
+    versions = ch_versions
+}
+
+
+def add_suffix(row, suffix) {
+    def meta = [:]
+    meta.id           = "${row[0].id}_${suffix}"
+    def array = []
+    array = [ meta, row[1] ]
+    return array
+}
\ No newline at end of file

From 944d27789fb85915efd6f5e60f43fcc77667a892 Mon Sep 17 00:00:00 2001
From: Christian Kubica <chriskub@CK-MBP14USIctx.local>
Date: Wed, 15 Jun 2022 09:47:40 +0200
Subject: [PATCH 008/227] INCLUDE UMITOOLS DEDUP

includes the optional umitools deduplication step after the read
QC.
---
 workflows/smrnaseq.nf | 29 +++++++++++++++++++++--------
 1 file changed, 21 insertions(+), 8 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 1613425c..27bf842a 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -54,12 +54,13 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature miRNA fasta file not found: ${params.mature}" }
 if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin miRNA fasta file not found: ${params.hairpin}" }
 
-include { INPUT_CHECK       } from '../subworkflows/local/input_check'
+include { INPUT_CHECK                } from '../subworkflows/local/input_check'
 include { FASTQC_UMITOOLS_TRIMGALORE } from '../subworkflows/nf-core/fastqc_umitools_trimgalore'
-include { MIRNA_QUANT       } from '../subworkflows/local/mirna_quant'
-include { GENOME_QUANT      } from '../subworkflows/local/genome_quant'
-include { MIRTRACE          } from '../subworkflows/local/mirtrace'
-include { MIRDEEP2          } from '../subworkflows/local/mirdeep2'
+include { DEDUPLICATE_UMIS           } from '../subworkflows/local/umi_dedup'
+include { MIRNA_QUANT                } from '../subworkflows/local/mirna_quant'
+include { GENOME_QUANT               } from '../subworkflows/local/genome_quant'
+include { MIRTRACE                   } from '../subworkflows/local/mirtrace'
+include { MIRDEEP2                   } from '../subworkflows/local/mirdeep2'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -135,14 +136,26 @@ workflow SMRNASEQ {
     //
     FASTQC_UMITOOLS_TRIMGALORE (
         ch_cat_fastq,
-        params.with_umi,
-        params.umi_discard_read,
         params.skip_fastqc || params.skip_qc,
-        params.skip_trimming
+        params.with_umi,
+        params.skip_trimming,
+        params.umi_discard_read
     )
     ch_versions = ch_versions.mix(FASTQC_UMITOOLS_TRIMGALORE.out.versions)
 
     reads_for_mirna = FASTQC_UMITOOLS_TRIMGALORE.out.reads
+
+    reads_for_mirna.view()
+
+    if (params.with_umi){
+        if (fasta){
+            fasta_ch = file(fasta)
+            DEDUPLICATE_UMIS (fasta_ch, bt_index, FASTQC_UMITOOLS_TRIMGALORE.out.reads)
+            reads_for_mirna = DEDUPLICATE_UMIS.out.reads
+            ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)
+        }
+    }
+
     MIRNA_QUANT (
         reference_mature,
         reference_hairpin,

From ddb3dbaa92b6243b3dc498290062fd3b7f761925 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Wed, 15 Jun 2022 13:55:52 +0200
Subject: [PATCH 009/227] ADD SAMTOOLS SORT CONFIG

Added additional configuration to change the output file name of
samtools sort.
---
 conf/modules.config | 14 +++++++++++++-
 1 file changed, 13 insertions(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index c1a74e21..92832223 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -172,8 +172,20 @@ if (params.with_umi) {
             ]
         }
 
+        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT' {
+            ext.prefix = { "${meta.id}.sorted" }
+            publishDir = [
+                path: { "${params.outdir}/umi_dedup" },
+                mode: params.publish_dir_mode,
+                pattern: '*.{bam}',
+                enabled: (
+                    params.save_umi_intermeds
+                )
+            ]
+        }
+
         withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX' {
-            ext.prefix = { "${meta.id}.umi_dedup.sorted" }
+            ext.prefix = { "${meta.id}.sorted" }
             publishDir = [
                 path: { "${params.outdir}/umi_dedup" },
                 mode: params.publish_dir_mode,

From b2ef66a76772f2299a705a10a42622dbc3d32407 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Wed, 15 Jun 2022 15:28:36 +0200
Subject: [PATCH 010/227] FIX TYPO

---
 subworkflows/local/umi_dedup.nf | 6 ------
 1 file changed, 6 deletions(-)

diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index f97b8e8c..5886ed52 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -30,8 +30,6 @@ workflow DEDUPLICATE_UMIS {
     }
 
     if (bt_indices){
-
-        reads.view()
         
         BOWTIE_MAP_GENOME ( reads, bt_indices.collect() )
         ch_versions = ch_versions.mix(BOWTIE_MAP_GENOME.out.versions)
@@ -39,11 +37,7 @@ workflow DEDUPLICATE_UMIS {
         BAM_SORT_SAMTOOLS ( BOWTIE_MAP_GENOME.out.bam, Channel.empty() )
         ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions)
 
-        BAM_SORT_SAMTOOLS.out.bam.view()
         ch_umi_dedup = BAM_SORT_SAMTOOLS.out.bam.join(BAM_SORT_SAMTOOLS.out.bai)
-
-        ch_umi_dedup.view()
-
         UMITOOLS_DEDUP ( ch_umi_dedup )
         ch_versions = ch_versions.mix(UMITOOLS_DEDUP.out.versions)
         ch_dedup_stats = ch_dedup_stats.mix(UMITOOLS_DEDUP.out.tsv_edit_distance).join(UMITOOLS_DEDUP.out.tsv_per_umi).join(UMITOOLS_DEDUP.out.tsv_umi_per_position)

From 29ec7dae7ec06fe74ec1fd0d4745e1e81d06087c Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Wed, 15 Jun 2022 15:28:47 +0200
Subject: [PATCH 011/227] ADD DEDUP DOCUMENTATION

Added the documentation detailing the output files of the UMI-tools
deduplication step.
---
 docs/output.md | 17 ++++++++++++++++-
 1 file changed, 16 insertions(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index 79f1d6d9..e0c2bd67 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -15,6 +15,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 - [FastQC](#fastqc) - read quality control
 - [UMI-tools extract](#umi-tools-extract) - UMI barcode extraction
 - [TrimGalore](#trimgalore) - adapter trimming
+- [UMI-tools deduplicate](#umi-tools-deduplicate) - read deduplication
 - [Bowtie](#bowtie) - alignment against mature miRNAs and miRNA precursors (hairpins)
 - [SAMtools](#samtools) - alignment result processing and feature counting
 - [edgeR](#edger) - normalization, MDS plot and sample pairwise distance heatmap
@@ -51,7 +52,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 </details>
 
-[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name. Secondly, reads are deduplicated based on UMI identifier after mapping as highlighted in the [UMI-tools dedup](#umi-tools-dedup) section.
+[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name. Secondly, reads are deduplicated based on UMI identifier after mapping as highlighted in the [UMI-tools deduplicate](#umi-tools-deduplicate) section.
 
 To facilitate processing of input data which has the UMI barcode already embedded in the read name from the start, `--skip_umi_extract` can be specified in conjunction with `--with_umi`.
 
@@ -74,6 +75,20 @@ This is an example of the output we can get:
 
 ![cutadapt](images/cutadapt_plot.png)
 
+## UMI-tools deduplicate
+
+<details markdown="1">
+<summary>Output files</summary>
+
+- `umi_dedup/`
+  - `*.tsv`: Results statistics files detailing the UMI deduplication results.
+  - `*.bam`: If `--save_umi_intermeds` is specified, the deduplicated bam files **after** UMI deduplication will be placed in this directory. In addition the sorted and indexed files will be placed there as well.
+  - `samtools_stats/`
+    - `*.{stats,flagstat,idxstats}:` Statistics on the mappings underlying the UMI deduplication.
+</details>
+
+[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name as highlighted in the [UMI-tools extract](#umi-tools-extract) section. The reads are deduplicated based on an alignment against the full genome of the species. The deduplicated reads are converted into fastq format and then used in the pipeline.
+
 ## Bowtie
 
 [Bowtie](http://bowtie-bio.sourceforge.net/index.shtml) is used for mapping adapter trimmed reads against the mature miRNAs and miRNA precursors (hairpins) in [miRBase](http://www.mirbase.org/).

From afa1ad76c7ece9d5a521e44a16abe522655c9808 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Wed, 15 Jun 2022 15:38:42 +0200
Subject: [PATCH 012/227] ADD DEDUP STEP

---
 README.md | 15 ++++++++-------
 1 file changed, 8 insertions(+), 7 deletions(-)

diff --git a/README.md b/README.md
index e4294f99..e3ceb707 100644
--- a/README.md
+++ b/README.md
@@ -35,24 +35,25 @@ On release, automated continuous integration tests run the pipeline on a full-si
 3. Adapter trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/))
    1. Insert Size calculation
    2. Collapse reads ([`seqcluster`](https://seqcluster.readthedocs.io/mirna_annotation.html#processing-of-reads))
-4. Alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-5. Alignment against miRBase hairpin
+4. UMI barcode deduplication ([`UMI-tools`](https://github.com/CGATOxford/UMI-tools))
+5. Alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
+6. Alignment against miRBase hairpin
    1. Unaligned reads from step 3 ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
    2. Collapsed reads from step 2.2 ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-6. Post-alignment processing of miRBase hairpin
+7. Post-alignment processing of miRBase hairpin
    1. Basic statistics from step 3 and step 4.1 ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
    2. Analysis on miRBase hairpin counts ([`edgeR`](https://bioconductor.org/packages/release/bioc/html/edgeR.html))
       - TMM normalization and a table of top expression hairpin
       - MDS plot clustering samples
       - Heatmap of sample similarities
    3. miRNA and isomiR annotation from step 4.1 ([`mirtop`](https://github.com/miRTop/mirtop))
-7. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
+8. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
    1. Post-alignment processing of alignment against host reference genome ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
-8. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation))
+9. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation))
    1. Mapping against reference genome with the mapper module
    2. Known and novel miRNA discovery with the mirdeep2 module
-9. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace))
-10. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
+10. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace))
+11. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
 
 ## Quick Start
 

From c72ac5be2993be2a65e90c0d4bc99385fd2d661b Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Wed, 15 Jun 2022 15:43:39 +0200
Subject: [PATCH 013/227] ADD UMITOOLS VERSION

---
 CHANGELOG.md | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index db1eec43..32df9aaf 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,7 +21,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### Other enhancements & fixes
 
 - [#134](https://github.com/nf-core/smrnaseq/issues/134) - Fixed colSum of zero issues for edgeR_miRBase.R script
-- [#49](https://github.com/nf-core/smrnaseq/issues/49) - Integrated the existing umitools modules into the pipeline.
+- [#49](https://github.com/nf-core/smrnaseq/issues/49) - Integrated the existing umitools modules into the pipeline and extend the deduplication step.
 
 ### Parameters
 
@@ -61,6 +61,7 @@ Note, since the pipeline is now using Nextflow DSL2, each process will be run wi
 | `seqkit`             | 0.16.0      | 2.0.0       |
 | `trim-galore`        | 0.6.6       | 0.6.7       |
 | `bioconvert`         | -           | 0.4.3       |
+| `umi_tools`          | -           | 1.1.2       |
 | `htseq`              | -           | -           |
 | `markdown`           | -           | -           |
 | `pymdown-extensions` | -           | -           |

From f44228931a3342e700b6e900e7ddfdcda91fc5d4 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Mon, 20 Jun 2022 15:26:09 +0200
Subject: [PATCH 014/227] MERGE DEDUPLICATED AND UNMAPPED READS AFTER
 DEDUPLICATION

After deduplication the reads that remained unaligned to the
provided reference genome are merged with the set of deduplicated
reads to enable the use of the full spectrum of reads, independent
of potential reference bias. This behaviour can be deactivated by
setting --umi_merge_unmapped false
---
 conf/modules.config             |  2 +-
 modules/local/join_reads.nf     | 21 +++++++++++++++++++++
 subworkflows/local/umi_dedup.nf | 25 +++++++++++++++++++------
 workflows/smrnaseq.nf           |  4 +---
 4 files changed, 42 insertions(+), 10 deletions(-)
 create mode 100644 modules/local/join_reads.nf

diff --git a/conf/modules.config b/conf/modules.config
index 92832223..ed78630e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -196,7 +196,7 @@ if (params.with_umi) {
             ]
         }
 
-        withName: '.*:DEDUPLICATE_UMIS:BAM_STATS_SAMTOOLS:.*' {
+        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:.*' {
             publishDir = [
                 path: { "${params.outdir}/umi_dedup/samtools_stats" },
                 mode: params.publish_dir_mode,
diff --git a/modules/local/join_reads.nf b/modules/local/join_reads.nf
new file mode 100644
index 00000000..9f68f062
--- /dev/null
+++ b/modules/local/join_reads.nf
@@ -0,0 +1,21 @@
+process JOIN_FASTQS {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? 'bioconda::samtools=1.13' : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/mulled-v2-ffbf83a6b0ab6ec567a336cf349b80637135bca3:40128b496751b037e2bd85f6789e83d4ff8a4837-0' :
+        'quay.io/biocontainers/mulled-v2-ffbf83a6b0ab6ec567a336cf349b80637135bca3:40128b496751b037e2bd85f6789e83d4ff8a4837-0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    tuple val(unmapped_meta), path(unmapped_reads)
+
+    output:
+    tuple val(meta), path('*_merged.fq.gz'), emit: merged
+    script:
+    """
+    cat ${reads} ${unmapped_reads} > ${meta.id}_merged.fq.gz
+    """
+
+}
diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 5886ed52..0e054d9b 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -3,10 +3,11 @@
 //
 
 include { INDEX_GENOME                        } from '../../modules/local/bowtie_genome'
-include { BOWTIE_MAP_SEQ as BOWTIE_MAP_GENOME } from '../../modules/local/bowtie_map_mirna'
+include { BOWTIE_MAP_SEQ as UMI_MAP_GENOME    } from '../../modules/local/bowtie_map_mirna'
 include { BAM_SORT_SAMTOOLS                   } from '../../subworkflows/nf-core/bam_sort_samtools'
 include { UMITOOLS_DEDUP                      } from '../../modules/nf-core/modules/umitools/dedup/main'
 include { SAMTOOLS_BAM2FQ                     } from '../../modules/nf-core/modules/samtools/bam2fq/main'
+include { JOIN_FASTQS                         } from '../../modules/local/join_reads'
 
 workflow DEDUPLICATE_UMIS {
     take:
@@ -31,10 +32,10 @@ workflow DEDUPLICATE_UMIS {
 
     if (bt_indices){
         
-        BOWTIE_MAP_GENOME ( reads, bt_indices.collect() )
-        ch_versions = ch_versions.mix(BOWTIE_MAP_GENOME.out.versions)
+        UMI_MAP_GENOME ( reads, bt_indices.collect() )
+        ch_versions = ch_versions.mix(UMI_MAP_GENOME.out.versions)
 
-        BAM_SORT_SAMTOOLS ( BOWTIE_MAP_GENOME.out.bam, Channel.empty() )
+        BAM_SORT_SAMTOOLS ( UMI_MAP_GENOME.out.bam, Channel.empty() )
         ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions)
 
         ch_umi_dedup = BAM_SORT_SAMTOOLS.out.bam.join(BAM_SORT_SAMTOOLS.out.bai)
@@ -44,12 +45,24 @@ workflow DEDUPLICATE_UMIS {
 
         SAMTOOLS_BAM2FQ ( UMITOOLS_DEDUP.out.bam, false )
         ch_versions = ch_versions.mix(SAMTOOLS_BAM2FQ.out.versions)
+
+        ch_dedup_reads = SAMTOOLS_BAM2FQ.out.reads
+
+        if ( params.umi_merge_unmapped ) {
+    
+            JOIN_FASTQS ( 
+                SAMTOOLS_BAM2FQ.out.reads,
+                UMI_MAP_GENOME.out.unmapped 
+            )
+            ch_dedup_reads = JOIN_FASTQS.out.merged
+        }
     }
 
     emit:
-    reads    = SAMTOOLS_BAM2FQ.out.reads // channel: [ val(meta), [ reads ] ]
+//    reads    = SAMTOOLS_BAM2FQ.out.reads // channel: [ val(meta), [ reads ] ]
+    reads    = ch_dedup_reads
     indices  = bt_indices
-    stats    = ch_dedup_stats
+//    stats    = ch_dedup_stats
     versions = ch_versions
 }
 
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 27bf842a..54f0e505 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -144,9 +144,7 @@ workflow SMRNASEQ {
     ch_versions = ch_versions.mix(FASTQC_UMITOOLS_TRIMGALORE.out.versions)
 
     reads_for_mirna = FASTQC_UMITOOLS_TRIMGALORE.out.reads
-
-    reads_for_mirna.view()
-
+    
     if (params.with_umi){
         if (fasta){
             fasta_ch = file(fasta)

From f9ca542f8d4710a3b779eda7e366b4ac70ffb2e7 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Mon, 20 Jun 2022 15:29:00 +0200
Subject: [PATCH 015/227] ADD MISSING OPTION

---
 nextflow.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/nextflow.config b/nextflow.config
index 4d724120..78df9386 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -34,6 +34,7 @@ params {
     umitools_bc_pattern        = null
     umi_discard_read           = null
     save_umi_intermeds         = false
+    umi_merge_unmapped         = true
 
     // Trimming options
     clip_r1                    = 0

From b9747170589a60215f57d04bf39426bd5fca7868 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Mon, 20 Jun 2022 15:29:36 +0200
Subject: [PATCH 016/227] ADD NEWLINE

---
 modules/local/mirdeep2_run.nf | 1 -
 1 file changed, 1 deletion(-)

diff --git a/modules/local/mirdeep2_run.nf b/modules/local/mirdeep2_run.nf
index 8ef6e804..5444ecfc 100644
--- a/modules/local/mirdeep2_run.nf
+++ b/modules/local/mirdeep2_run.nf
@@ -37,4 +37,3 @@ process MIRDEEP2_RUN {
     END_VERSIONS
     """
 }
-

From 4610be12d815689d0918f8c335b45dd6cfbd0107 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Tue, 21 Jun 2022 10:20:00 +0200
Subject: [PATCH 017/227] CLEAN CODE

---
 subworkflows/local/umi_dedup.nf | 12 +-----------
 1 file changed, 1 insertion(+), 11 deletions(-)

diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 0e054d9b..94341a96 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -59,18 +59,8 @@ workflow DEDUPLICATE_UMIS {
     }
 
     emit:
-//    reads    = SAMTOOLS_BAM2FQ.out.reads // channel: [ val(meta), [ reads ] ]
     reads    = ch_dedup_reads
     indices  = bt_indices
-//    stats    = ch_dedup_stats
+    stats    = ch_dedup_stats
     versions = ch_versions
 }
-
-
-def add_suffix(row, suffix) {
-    def meta = [:]
-    meta.id           = "${row[0].id}_${suffix}"
-    def array = []
-    array = [ meta, row[1] ]
-    return array
-}
\ No newline at end of file

From 67b2caceadeae51cdfc86e5a966dbeee66a4e69f Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Tue, 21 Jun 2022 10:21:45 +0200
Subject: [PATCH 018/227] ADD DOCUMENTATION

---
 workflows/smrnaseq.nf | 5 ++++-
 1 file changed, 4 insertions(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 54f0e505..51e412e8 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -144,7 +144,10 @@ workflow SMRNASEQ {
     ch_versions = ch_versions.mix(FASTQC_UMITOOLS_TRIMGALORE.out.versions)
 
     reads_for_mirna = FASTQC_UMITOOLS_TRIMGALORE.out.reads
-    
+
+    //
+    // SUBWORKFLOW: Deduplicate UMIs by mapping them to the genome
+    //
     if (params.with_umi){
         if (fasta){
             fasta_ch = file(fasta)

From 23fc985ab537abe0aa55771a693e831e6eae9f92 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Tue, 21 Jun 2022 10:25:34 +0200
Subject: [PATCH 019/227] ADD UMI_MERGE_UNMAPPED COMMAND

---
 nextflow_schema.json | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 5019408f..4547be90 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -88,6 +88,11 @@
                     "type": "boolean",
                     "fa_icon": "fas fa-save",
                     "description": "If this option is specified, intermediate FastQ and BAM files produced by UMI-tools are also saved in the results directory."
+                },
+                "umi_merge_unmapped": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-save",
+                    "description": "Unless deactivated the deduplicated reads are merged with the reads that could not be placed to reduce the potential reference bias"
                 }
             },
             "fa_icon": "fas fa-barcode"

From be241eafd97c5943be3614a0b843e28fec6b5be3 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Tue, 21 Jun 2022 10:31:32 +0200
Subject: [PATCH 020/227] FINALIZE DOCUMENTATION

Information on the new --umi_merge_unmapped command were added to
both the CHANGELOG, as well as the output markdown script.
---
 CHANGELOG.md   | 1 +
 docs/output.md | 2 +-
 2 files changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 32df9aaf..8fb364d4 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -35,6 +35,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 |                      | `--umitools_bc_pattern`     |
 |                      | `--umi_discard_read`        |
 |                      | `--save_umi_intermeds`      |
+|                      | `--umi_merge_unmapped`      |
 
 > **NB:** Parameter has been **updated** if both old and new parameter information is present.
 > **NB:** Parameter has been **added** if just the new parameter information is present.
diff --git a/docs/output.md b/docs/output.md
index e0c2bd67..14cc4e6d 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -87,7 +87,7 @@ This is an example of the output we can get:
     - `*.{stats,flagstat,idxstats}:` Statistics on the mappings underlying the UMI deduplication.
 </details>
 
-[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name as highlighted in the [UMI-tools extract](#umi-tools-extract) section. The reads are deduplicated based on an alignment against the full genome of the species. The deduplicated reads are converted into fastq format and then used in the pipeline.
+[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name as highlighted in the [UMI-tools extract](#umi-tools-extract) section. The reads are deduplicated based on an alignment against the full genome of the species. The deduplicated reads are then converted into fastq format and merged with the reads that remained unmapped in order to reduce potential reference bias. This behavior can be stopped by setting `--umi_merge_unmapped false`. The resulting fastq files are used in the remaining steps of the pipeline.
 
 ## Bowtie
 

From 8b433f1876749f277cf946f84b52407434fdcbdc Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Tue, 21 Jun 2022 10:37:09 +0200
Subject: [PATCH 021/227] UPDATE MAIL TEMPLATE

---
 assets/email_template.html | 142 +++++++++++--------------------------
 1 file changed, 42 insertions(+), 100 deletions(-)

diff --git a/assets/email_template.html b/assets/email_template.html
index 05d590d3..e75e86ac 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -1,111 +1,53 @@
 <html>
-  <head>
-    <meta charset="utf-8" />
-    <meta http-equiv="X-UA-Compatible" content="IE=edge" />
-    <meta name="viewport" content="width=device-width, initial-scale=1" />
+<head>
+  <meta charset="utf-8">
+  <meta http-equiv="X-UA-Compatible" content="IE=edge">
+  <meta name="viewport" content="width=device-width, initial-scale=1">
 
-    <!-- prettier-ignore -->
-    <meta name="description" content="nf-core/smrnaseq: Small RNA-Seq Best Practice Analysis Pipeline." />
-    <title>nf-core/smrnaseq Pipeline Report</title>
-  </head>
-  <body>
-    <div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto">
-      <img src="cid:nfcorepipelinelogo" />
+  <meta name="description" content="nf-core/smrnaseq: Small RNA-Seq Best Practice Analysis Pipeline.">
+  <title>nf-core/smrnaseq Pipeline Report</title>
+</head>
+<body>
+<div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto;">
 
-      <h1>nf-core/smrnaseq v${version}</h1>
-      <h2>Run Name: $runName</h2>
+<img src="cid:nfcorepipelinelogo">
 
-      <% if (!success){ out << """
-      <div
-        style="
-          color: #a94442;
-          background-color: #f2dede;
-          border-color: #ebccd1;
-          padding: 15px;
-          margin-bottom: 20px;
-          border: 1px solid transparent;
-          border-radius: 4px;
-        "
-      >
-        <h4 style="margin-top: 0; color: inherit">nf-core/smrnaseq execution completed unsuccessfully!</h4>
+<h1>nf-core/smrnaseq v${version}</h1>
+<h2>Run Name: $runName</h2>
+
+<% if (!success){
+    out << """
+    <div style="color: #a94442; background-color: #f2dede; border-color: #ebccd1; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
+        <h4 style="margin-top:0; color: inherit;">nf-core/smrnaseq execution completed unsuccessfully!</h4>
         <p>The exit status of the task that caused the workflow execution to fail was: <code>$exitStatus</code>.</p>
         <p>The full error message was:</p>
-        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0">${errorReport}</pre>
-      </div>
-      """ } else { out << """
-      <div
-        style="
-          color: #3c763d;
-          background-color: #dff0d8;
-          border-color: #d6e9c6;
-          padding: 15px;
-          margin-bottom: 20px;
-          border: 1px solid transparent;
-          border-radius: 4px;
-        "
-      >
+        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0;">${errorReport}</pre>
+    </div>
+    """
+} else {
+    out << """
+    <div style="color: #3c763d; background-color: #dff0d8; border-color: #d6e9c6; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
         nf-core/smrnaseq execution completed successfully!
-      </div>
-      """ } %>
+    </div>
+    """
+}
+%>
 
-      <p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
-      <p>The command used to launch the workflow was as follows:</p>
-      <pre
-        style="
-          white-space: pre-wrap;
-          overflow: visible;
-          background-color: #ededed;
-          padding: 15px;
-          border-radius: 4px;
-          margin-bottom: 30px;
-        "
-      >
-$commandLine</pre
-      >
+<p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
+<p>The command used to launch the workflow was as follows:</p>
+<pre style="white-space: pre-wrap; overflow: visible; background-color: #ededed; padding: 15px; border-radius: 4px; margin-bottom:30px;">$commandLine</pre>
 
-      <h3>Pipeline Configuration:</h3>
-      <table
-        style="
-          width: 100%;
-          max-width: 100%;
-          border-spacing: 0;
-          border-collapse: collapse;
-          border: 0;
-          margin-bottom: 30px;
-        "
-      >
-        <tbody style="border-bottom: 1px solid #ddd">
-          <% out << summary.collect{ k,v -> "
-          <tr>
-            <th
-              style="
-                text-align: left;
-                padding: 8px 0;
-                line-height: 1.42857143;
-                vertical-align: top;
-                border-top: 1px solid #ddd;
-              "
-            >
-              $k
-            </th>
-            <td
-              style="
-                text-align: left;
-                padding: 8px;
-                line-height: 1.42857143;
-                vertical-align: top;
-                border-top: 1px solid #ddd;
-              "
-            >
-              <pre style="white-space: pre-wrap; overflow: visible">$v</pre>
-            </td>
-          </tr>
-          " }.join("\n") %>
-        </tbody>
-      </table>
+<h3>Pipeline Configuration:</h3>
+<table style="width:100%; max-width:100%; border-spacing: 0; border-collapse: collapse; border:0; margin-bottom: 30px;">
+    <tbody style="border-bottom: 1px solid #ddd;">
+        <% out << summary.collect{ k,v -> "<tr><th style='text-align:left; padding: 8px 0; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'>$k</th><td style='text-align:left; padding: 8px; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'><pre style='white-space: pre-wrap; overflow: visible;'>$v</pre></td></tr>" }.join("\n") %>
+    </tbody>
+</table>
 
-      <p>nf-core/smrnaseq</p>
-      <p><a href="https://github.com/nf-core/smrnaseq">https://github.com/nf-core/smrnaseq</a></p>
-    </div>
-  </body>
+<p>nf-core/smrnaseq</p>
+<p><a href="https://github.com/nf-core/smrnaseq">https://github.com/nf-core/smrnaseq</a></p>
+
+</div>
+
+</body>
 </html>

From 0e732ede28b931b4e233d07cc092526753de95c1 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Tue, 21 Jun 2022 10:43:38 +0200
Subject: [PATCH 022/227] CHANGE DAG OUTPUT TO HTML

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 78df9386..7e528b38 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -192,7 +192,7 @@ trace {
 }
 dag {
     enabled = true
-    file    = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg"
+    file    = "${params.tracedir}/pipeline_dag_${trace_timestamp}.html"
 }
 
 manifest {

From 8f426b56ac14b90bef00aa63ff57ecdb6c843e1f Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Tue, 21 Jun 2022 10:56:12 +0200
Subject: [PATCH 023/227] PLEASE PRETTIER

---
 assets/email_template.html | 141 ++++++++++++++++++++++++++-----------
 docs/output.md             |   5 +-
 2 files changed, 101 insertions(+), 45 deletions(-)

diff --git a/assets/email_template.html b/assets/email_template.html
index e75e86ac..d856192a 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -1,53 +1,110 @@
 <html>
-<head>
-  <meta charset="utf-8">
-  <meta http-equiv="X-UA-Compatible" content="IE=edge">
-  <meta name="viewport" content="width=device-width, initial-scale=1">
+  <head>
+    <meta charset="utf-8" />
+    <meta http-equiv="X-UA-Compatible" content="IE=edge" />
+    <meta name="viewport" content="width=device-width, initial-scale=1" />
 
-  <meta name="description" content="nf-core/smrnaseq: Small RNA-Seq Best Practice Analysis Pipeline.">
-  <title>nf-core/smrnaseq Pipeline Report</title>
-</head>
-<body>
-<div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto;">
+    <meta name="description" content="nf-core/smrnaseq: Small RNA-Seq Best Practice Analysis Pipeline." />
+    <title>nf-core/smrnaseq Pipeline Report</title>
+  </head>
+  <body>
+    <div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto">
+      <img src="cid:nfcorepipelinelogo" />
 
-<img src="cid:nfcorepipelinelogo">
+      <h1>nf-core/smrnaseq v${version}</h1>
+      <h2>Run Name: $runName</h2>
 
-<h1>nf-core/smrnaseq v${version}</h1>
-<h2>Run Name: $runName</h2>
-
-<% if (!success){
-    out << """
-    <div style="color: #a94442; background-color: #f2dede; border-color: #ebccd1; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
-        <h4 style="margin-top:0; color: inherit;">nf-core/smrnaseq execution completed unsuccessfully!</h4>
+      <% if (!success){ out << """
+      <div
+        style="
+          color: #a94442;
+          background-color: #f2dede;
+          border-color: #ebccd1;
+          padding: 15px;
+          margin-bottom: 20px;
+          border: 1px solid transparent;
+          border-radius: 4px;
+        "
+      >
+        <h4 style="margin-top: 0; color: inherit">nf-core/smrnaseq execution completed unsuccessfully!</h4>
         <p>The exit status of the task that caused the workflow execution to fail was: <code>$exitStatus</code>.</p>
         <p>The full error message was:</p>
-        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0;">${errorReport}</pre>
-    </div>
-    """
-} else {
-    out << """
-    <div style="color: #3c763d; background-color: #dff0d8; border-color: #d6e9c6; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
+        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0">${errorReport}</pre>
+      </div>
+      """ } else { out << """
+      <div
+        style="
+          color: #3c763d;
+          background-color: #dff0d8;
+          border-color: #d6e9c6;
+          padding: 15px;
+          margin-bottom: 20px;
+          border: 1px solid transparent;
+          border-radius: 4px;
+        "
+      >
         nf-core/smrnaseq execution completed successfully!
-    </div>
-    """
-}
-%>
+      </div>
+      """ } %>
 
-<p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
-<p>The command used to launch the workflow was as follows:</p>
-<pre style="white-space: pre-wrap; overflow: visible; background-color: #ededed; padding: 15px; border-radius: 4px; margin-bottom:30px;">$commandLine</pre>
+      <p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
+      <p>The command used to launch the workflow was as follows:</p>
+      <pre
+        style="
+          white-space: pre-wrap;
+          overflow: visible;
+          background-color: #ededed;
+          padding: 15px;
+          border-radius: 4px;
+          margin-bottom: 30px;
+        "
+      >
+$commandLine</pre
+      >
 
-<h3>Pipeline Configuration:</h3>
-<table style="width:100%; max-width:100%; border-spacing: 0; border-collapse: collapse; border:0; margin-bottom: 30px;">
-    <tbody style="border-bottom: 1px solid #ddd;">
-        <% out << summary.collect{ k,v -> "<tr><th style='text-align:left; padding: 8px 0; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'>$k</th><td style='text-align:left; padding: 8px; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'><pre style='white-space: pre-wrap; overflow: visible;'>$v</pre></td></tr>" }.join("\n") %>
-    </tbody>
-</table>
+      <h3>Pipeline Configuration:</h3>
+      <table
+        style="
+          width: 100%;
+          max-width: 100%;
+          border-spacing: 0;
+          border-collapse: collapse;
+          border: 0;
+          margin-bottom: 30px;
+        "
+      >
+        <tbody style="border-bottom: 1px solid #ddd">
+          <% out << summary.collect{ k,v -> "
+          <tr>
+            <th
+              style="
+                text-align: left;
+                padding: 8px 0;
+                line-height: 1.42857143;
+                vertical-align: top;
+                border-top: 1px solid #ddd;
+              "
+            >
+              $k
+            </th>
+            <td
+              style="
+                text-align: left;
+                padding: 8px;
+                line-height: 1.42857143;
+                vertical-align: top;
+                border-top: 1px solid #ddd;
+              "
+            >
+              <pre style="white-space: pre-wrap; overflow: visible">$v</pre>
+            </td>
+          </tr>
+          " }.join("\n") %>
+        </tbody>
+      </table>
 
-<p>nf-core/smrnaseq</p>
-<p><a href="https://github.com/nf-core/smrnaseq">https://github.com/nf-core/smrnaseq</a></p>
-
-</div>
-
-</body>
+      <p>nf-core/smrnaseq</p>
+      <p><a href="https://github.com/nf-core/smrnaseq">https://github.com/nf-core/smrnaseq</a></p>
+    </div>
+  </body>
 </html>
diff --git a/docs/output.md b/docs/output.md
index 14cc4e6d..c2b28d06 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -83,9 +83,8 @@ This is an example of the output we can get:
 - `umi_dedup/`
   - `*.tsv`: Results statistics files detailing the UMI deduplication results.
   - `*.bam`: If `--save_umi_intermeds` is specified, the deduplicated bam files **after** UMI deduplication will be placed in this directory. In addition the sorted and indexed files will be placed there as well.
-  - `samtools_stats/`
-    - `*.{stats,flagstat,idxstats}:` Statistics on the mappings underlying the UMI deduplication.
-</details>
+  - `samtools_stats/` - `*.{stats,flagstat,idxstats}:` Statistics on the mappings underlying the UMI deduplication.
+  </details>
 
 [UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name as highlighted in the [UMI-tools extract](#umi-tools-extract) section. The reads are deduplicated based on an alignment against the full genome of the species. The deduplicated reads are then converted into fastq format and merged with the reads that remained unmapped in order to reduce potential reference bias. This behavior can be stopped by setting `--umi_merge_unmapped false`. The resulting fastq files are used in the remaining steps of the pipeline.
 

From 039843f42d61801163491d4518ec295203ba9a1f Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Tue, 21 Jun 2022 13:06:41 +0200
Subject: [PATCH 024/227] FIX MERGE ERROR

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index f66c3a7c..043796d9 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -166,7 +166,7 @@ workflow SMRNASEQ {
         }
     }
 
-    reads_for_mirna = FASTQC_TRIMGALORE.out.reads
+    reads_for_mirna = FASTQC_UMITOOLS_TRIMGALORE.out.reads
     //
     // SUBWORKFLOW: remove contaminants from reads
     //

From 53c097c366ca885ab6b7145a33de48a5f1c7fff6 Mon Sep 17 00:00:00 2001
From: CKComputomics <christian.kubica@computomics.com>
Date: Tue, 21 Jun 2022 13:12:22 +0200
Subject: [PATCH 025/227] MAKE PRETTIER HAPPY

---
 CHANGELOG.md | 29 ++++++++++++++---------------
 README.md    |  8 ++++----
 2 files changed, 18 insertions(+), 19 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 03ae1048..cc0ba654 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -16,20 +16,20 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Parameters
 
-| Old parameter | New parameter            |
-| ------------- | ------------------------ |
-|               | `--mirGeneDB`            |
-|               | `--mirGeneDB_species`    |
-|               | `--mirGeneDB_gff`        |
-|               | `--mirGeneDB_mature`     |
-|               | `--mirGeneDB_hairpin`    |
-|               | `--contamination_filter` |
-|               | `--rrna`                 |
-|               | `--trna`                 |
-|               | `--cdna`                 |
-|               | `--ncrna`                |
-|               | `--pirna`                |
-|               | `--other_contamination`  |
+| Old parameter | New parameter               |
+| ------------- | --------------------------- |
+|               | `--mirGeneDB`               |
+|               | `--mirGeneDB_species`       |
+|               | `--mirGeneDB_gff`           |
+|               | `--mirGeneDB_mature`        |
+|               | `--mirGeneDB_hairpin`       |
+|               | `--contamination_filter`    |
+|               | `--rrna`                    |
+|               | `--trna`                    |
+|               | `--cdna`                    |
+|               | `--ncrna`                   |
+|               | `--pirna`                   |
+|               | `--other_contamination`     |
 |               | `--with_umi`                |
 |               | `--umitools_extract_method` |
 |               | `--umitools_bc_pattern`     |
@@ -37,7 +37,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 |               | `--save_umi_intermeds`      |
 |               | `--umi_merge_unmapped`      |
 
-
 ## [v2.0.0](https://github.com/nf-core/smrnaseq/releases/tag/2.0.0) - 2022-05-31 Aqua Zinc Chihuahua
 
 ### Major enhancements
diff --git a/README.md b/README.md
index 5177598a..f6198652 100644
--- a/README.md
+++ b/README.md
@@ -48,10 +48,10 @@ On release, automated continuous integration tests run the pipeline on a full-si
 9. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
    1. Post-alignment processing of alignment against host reference genome ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
 10. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation))
-   1. Mapping against reference genome with the mapper module
-   2. Known and novel miRNA discovery with the mirdeep2 module
-11. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace))
-12. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
+11. Mapping against reference genome with the mapper module
+12. Known and novel miRNA discovery with the mirdeep2 module
+13. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace))
+14. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
 
 ## Quick Start
 

From 608c4140a989b567dd472fb852c08984eac4e2d0 Mon Sep 17 00:00:00 2001
From: Christian Kubica <christian.kubica@computomics.com>
Date: Wed, 22 Jun 2022 15:26:29 +0200
Subject: [PATCH 026/227] ADD NF-CORE CAT

Includes the nf-core cat module to replace the custom concatenation
module.
---
 modules.json                             |  3 ++
 modules/nf-core/modules/cat/cat/main.nf  | 62 ++++++++++++++++++++++++
 modules/nf-core/modules/cat/cat/meta.yml | 37 ++++++++++++++
 3 files changed, 102 insertions(+)
 create mode 100644 modules/nf-core/modules/cat/cat/main.nf
 create mode 100644 modules/nf-core/modules/cat/cat/meta.yml

diff --git a/modules.json b/modules.json
index c328879e..c74fa935 100644
--- a/modules.json
+++ b/modules.json
@@ -3,6 +3,9 @@
     "homePage": "https://github.com/nf-core/smrnaseq",
     "repos": {
         "nf-core/modules": {
+            "cat/cat": {
+                "git_sha": "eeda4136c096688d04cc40bb3c70d948213ed641"
+            },
             "cat/fastq": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
diff --git a/modules/nf-core/modules/cat/cat/main.nf b/modules/nf-core/modules/cat/cat/main.nf
new file mode 100644
index 00000000..40e53f3e
--- /dev/null
+++ b/modules/nf-core/modules/cat/cat/main.nf
@@ -0,0 +1,62 @@
+process CAT_CAT {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda (params.enable_conda ? "conda-forge::pigz=2.3.4" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/pigz:2.3.4' :
+        'quay.io/biocontainers/pigz:2.3.4' }"
+
+    input:
+    tuple val(meta), path(files_in)
+
+    output:
+    tuple val(meta), path("${prefix}"), emit: file_out
+    path "versions.yml"               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def file_list = files_in.collect { it.toString() }
+
+    // | input     | output     | command1 | command2 |
+    // |-----------|------------|----------|----------|
+    // | gzipped   | gzipped    | cat      |          |
+    // | ungzipped | ungzipped  | cat      |          |
+    // | gzipped   | ungzipped  | zcat     |          |
+    // | ungzipped | gzipped    | cat      | pigz     |
+
+    // Use input file ending as default
+    prefix   = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
+    out_zip  = prefix.endsWith('.gz')
+    in_zip   = file_list[0].endsWith('.gz')
+    command1 = (in_zip && !out_zip) ? 'zcat' : 'cat'
+    command2 = (!in_zip && out_zip) ? "| pigz -c -p $task.cpus $args2" : ''
+    """
+    $command1 \\
+        $args \\
+        ${file_list.join(' ')} \\
+        $command2 \\
+        > ${prefix}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
+    END_VERSIONS
+    """
+
+    stub:
+    def file_list = files_in.collect { it.toString() }
+    prefix   = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
+    """
+    touch $prefix
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/cat/cat/meta.yml b/modules/nf-core/modules/cat/cat/meta.yml
new file mode 100644
index 00000000..5eeff5a6
--- /dev/null
+++ b/modules/nf-core/modules/cat/cat/meta.yml
@@ -0,0 +1,37 @@
+name: cat_cat
+description: A module for concatenation of gzipped or uncompressed files
+keywords:
+  - concatenate
+  - gzip
+  - cat
+tools:
+  - cat:
+      description: Just concatenation
+      homepage: None
+      documentation: https://man7.org/linux/man-pages/man1/cat.1.html
+      tool_dev_url: None
+      licence: ["GPL-3.0-or-later"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - files_in:
+      type: file
+      description: List of compressed / uncompressed files
+      pattern: "*"
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - file_out:
+      type: file
+      description: Concatenated file. Will be gzipped if file_out ends with ".gz"
+      pattern: "${file_out}"
+
+authors:
+  - "@erikrikarddaniel"
+  - "@FriederikeHanssen"

From 6d305c25ba933082b8a6003a8670fe62c032ad1b Mon Sep 17 00:00:00 2001
From: Christian Kubica <christian.kubica@computomics.com>
Date: Wed, 22 Jun 2022 15:27:32 +0200
Subject: [PATCH 027/227] REPLACE CUSTOM CAT WITH NF-CORE CAT

Implements the use of the nf-core cat module.
---
 subworkflows/local/umi_dedup.nf | 24 +++++++++++++-----------
 workflows/smrnaseq.nf           |  7 +++++--
 2 files changed, 18 insertions(+), 13 deletions(-)

diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 94341a96..12033274 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -7,7 +7,7 @@ include { BOWTIE_MAP_SEQ as UMI_MAP_GENOME    } from '../../modules/local/bowtie
 include { BAM_SORT_SAMTOOLS                   } from '../../subworkflows/nf-core/bam_sort_samtools'
 include { UMITOOLS_DEDUP                      } from '../../modules/nf-core/modules/umitools/dedup/main'
 include { SAMTOOLS_BAM2FQ                     } from '../../modules/nf-core/modules/samtools/bam2fq/main'
-include { JOIN_FASTQS                         } from '../../modules/local/join_reads'
+include { CAT_CAT                             } from '../../modules/nf-core/modules/cat/cat/main'
 
 workflow DEDUPLICATE_UMIS {
     take:
@@ -22,17 +22,17 @@ workflow DEDUPLICATE_UMIS {
 
     if (!bt_index){
         INDEX_GENOME ( fasta )
-        bt_indices      = INDEX_GENOME.out.bt_indices
+        bt_index      = INDEX_GENOME.out.bowtie_indices
         fasta_formatted = INDEX_GENOME.out.fasta
         ch_versions     = ch_versions.mix(INDEX_GENOME.out.versions)
     } else {
-        bt_indices      = Channel.fromPath("${bt_index}**ebwt", checkIfExists: true).ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_index}" }
+        bt_index     = Channel.fromPath("${bt_index}**ebwt", checkIfExists: true).ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_index}" }
         fasta_formatted = fasta
     }
 
-    if (bt_indices){
+    if (bt_index){
         
-        UMI_MAP_GENOME ( reads, bt_indices.collect() )
+        UMI_MAP_GENOME ( reads, bt_index.collect() )
         ch_versions = ch_versions.mix(UMI_MAP_GENOME.out.versions)
 
         BAM_SORT_SAMTOOLS ( UMI_MAP_GENOME.out.bam, Channel.empty() )
@@ -49,18 +49,20 @@ workflow DEDUPLICATE_UMIS {
         ch_dedup_reads = SAMTOOLS_BAM2FQ.out.reads
 
         if ( params.umi_merge_unmapped ) {
+
+            SAMTOOLS_BAM2FQ.out.reads
+                .join(UMI_MAP_GENOME.out.unmapped)
+                .map { meta, file1, file2 -> [meta, [file1, file2]]}
+                .set { ch_cat }
     
-            JOIN_FASTQS ( 
-                SAMTOOLS_BAM2FQ.out.reads,
-                UMI_MAP_GENOME.out.unmapped 
-            )
-            ch_dedup_reads = JOIN_FASTQS.out.merged
+            CAT_CAT ( ch_cat )
+            ch_dedup_reads = CAT_CAT.out.file_out
         }
     }
 
     emit:
     reads    = ch_dedup_reads
-    indices  = bt_indices
+    indices  = bt_index
     stats    = ch_dedup_stats
     versions = ch_versions
 }
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 043796d9..dc30e875 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -160,13 +160,16 @@ workflow SMRNASEQ {
     if (params.with_umi){
         if (fasta){
             fasta_ch = file(fasta)
-            DEDUPLICATE_UMIS (fasta_ch, bt_index, FASTQC_UMITOOLS_TRIMGALORE.out.reads)
+            DEDUPLICATE_UMIS (
+                fasta_ch, 
+                bt_index, 
+                FASTQC_UMITOOLS_TRIMGALORE.out.reads
+            )
             reads_for_mirna = DEDUPLICATE_UMIS.out.reads
             ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)
         }
     }
 
-    reads_for_mirna = FASTQC_UMITOOLS_TRIMGALORE.out.reads
     //
     // SUBWORKFLOW: remove contaminants from reads
     //

From 57a8dbaab7f3614899952f4f880f6dcfa8c1c242 Mon Sep 17 00:00:00 2001
From: Christian Kubica <christian.kubica@computomics.com>
Date: Wed, 22 Jun 2022 15:30:58 +0200
Subject: [PATCH 028/227] REMOVE UNUSED MODULE

deletes the now unused conatenation module.
---
 modules/local/join_reads.nf | 21 ---------------------
 1 file changed, 21 deletions(-)
 delete mode 100644 modules/local/join_reads.nf

diff --git a/modules/local/join_reads.nf b/modules/local/join_reads.nf
deleted file mode 100644
index 9f68f062..00000000
--- a/modules/local/join_reads.nf
+++ /dev/null
@@ -1,21 +0,0 @@
-process JOIN_FASTQS {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda (params.enable_conda ? 'bioconda::samtools=1.13' : null)
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-ffbf83a6b0ab6ec567a336cf349b80637135bca3:40128b496751b037e2bd85f6789e83d4ff8a4837-0' :
-        'quay.io/biocontainers/mulled-v2-ffbf83a6b0ab6ec567a336cf349b80637135bca3:40128b496751b037e2bd85f6789e83d4ff8a4837-0' }"
-
-    input:
-    tuple val(meta), path(reads)
-    tuple val(unmapped_meta), path(unmapped_reads)
-
-    output:
-    tuple val(meta), path('*_merged.fq.gz'), emit: merged
-    script:
-    """
-    cat ${reads} ${unmapped_reads} > ${meta.id}_merged.fq.gz
-    """
-
-}

From 04b852cec33782bc51b05e5ed555cb93787d3871 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Thu, 12 Oct 2023 09:06:58 +0100
Subject: [PATCH 029/227] Re-organized code mirna_quant to make it simpler to
 read

---
 subworkflows/local/mirna_quant.nf | 37 +++++++++++++++++++++----------
 1 file changed, 25 insertions(+), 12 deletions(-)

diff --git a/subworkflows/local/mirna_quant.nf b/subworkflows/local/mirna_quant.nf
index dfa16ab4..e26839bc 100644
--- a/subworkflows/local/mirna_quant.nf
+++ b/subworkflows/local/mirna_quant.nf
@@ -33,24 +33,17 @@ workflow MIRNA_QUANT {
     main:
     ch_versions = Channel.empty()
 
+
+
     PARSE_MATURE ( mature ).parsed_fasta.set { mirna_parsed }
     ch_versions = ch_versions.mix(PARSE_MATURE.out.versions)
 
     FORMAT_MATURE ( mirna_parsed )
     ch_versions = ch_versions.mix(FORMAT_MATURE.out.versions)
 
-    PARSE_HAIRPIN ( hairpin ).parsed_fasta.set { hairpin_parsed }
-    ch_versions = ch_versions.mix(PARSE_HAIRPIN.out.versions)
-
-    FORMAT_HAIRPIN ( hairpin_parsed )
-    ch_versions = ch_versions.mix(FORMAT_HAIRPIN.out.versions)
-
     INDEX_MATURE ( FORMAT_MATURE.out.formatted_fasta ).index.set { mature_bowtie }
     ch_versions = ch_versions.mix(INDEX_MATURE.out.versions)
 
-    INDEX_HAIRPIN ( FORMAT_HAIRPIN.out.formatted_fasta ).index.set { hairpin_bowtie }
-    ch_versions = ch_versions.mix(INDEX_HAIRPIN.out.versions)
-
     reads
         .map { add_suffix(it, "mature") }
         .dump (tag:'msux')
@@ -64,15 +57,28 @@ workflow MIRNA_QUANT {
         .dump (tag:'hsux')
         .set { reads_hairpin }
 
-    BOWTIE_MAP_HAIRPIN ( reads_hairpin, hairpin_bowtie.collect() )
-    ch_versions = ch_versions.mix(BOWTIE_MAP_HAIRPIN.out.versions)
-
     BAM_STATS_MATURE ( BOWTIE_MAP_MATURE.out.bam, FORMAT_MATURE.out.formatted_fasta )
     ch_versions = ch_versions.mix(BAM_STATS_MATURE.out.versions)
 
+
+
+    PARSE_HAIRPIN ( hairpin ).parsed_fasta.set { hairpin_parsed }
+    ch_versions = ch_versions.mix(PARSE_HAIRPIN.out.versions)
+
+    FORMAT_HAIRPIN ( hairpin_parsed )
+    ch_versions = ch_versions.mix(FORMAT_HAIRPIN.out.versions)
+
+    INDEX_HAIRPIN ( FORMAT_HAIRPIN.out.formatted_fasta ).index.set { hairpin_bowtie }
+    ch_versions = ch_versions.mix(INDEX_HAIRPIN.out.versions)
+
+    BOWTIE_MAP_HAIRPIN ( reads_hairpin, hairpin_bowtie.collect() )
+    ch_versions = ch_versions.mix(BOWTIE_MAP_HAIRPIN.out.versions)
+
     BAM_STATS_HAIRPIN ( BOWTIE_MAP_HAIRPIN.out.bam, FORMAT_HAIRPIN.out.formatted_fasta )
     ch_versions = ch_versions.mix(BAM_STATS_HAIRPIN.out.versions)
 
+
+
     BAM_STATS_MATURE.out.idxstats.collect{it[1]}
         .mix(BAM_STATS_HAIRPIN.out.idxstats.collect{it[1]})
         .dump(tag:'edger')
@@ -81,6 +87,8 @@ workflow MIRNA_QUANT {
         .set { edger_input }
     EDGER_QC ( edger_input )
 
+
+
     reads
         .map { add_suffix(it, "seqcluster") }
         .dump (tag:'ssux')
@@ -92,6 +100,9 @@ workflow MIRNA_QUANT {
     BOWTIE_MAP_SEQCLUSTER ( reads_collapsed, hairpin_bowtie.collect() )
     ch_versions = ch_versions.mix(BOWTIE_MAP_SEQCLUSTER.out.versions)
 
+
+
+
     ch_mirtop_logs = Channel.empty()
     if (params.mirtrace_species){
         MIRTOP_QUANT ( BOWTIE_MAP_SEQCLUSTER.out.bam.collect{it[1]}, FORMAT_HAIRPIN.out.formatted_fasta.collect{it[1]}, gtf )
@@ -106,6 +117,8 @@ workflow MIRNA_QUANT {
         .dump (tag:'gsux')
         .set { reads_genome }
 
+
+
     emit:
     fasta_mature        = FORMAT_MATURE.out.formatted_fasta
     fasta_hairpin       = FORMAT_HAIRPIN.out.formatted_fasta

From f285867b7a1e370148a0de57338fdd7c5a04af6e Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Thu, 12 Oct 2023 09:07:47 +0100
Subject: [PATCH 030/227] Switched to HTML less hairpin.fa and mature.fa

---
 conf/test.config     | 4 ++--
 nextflow.config      | 4 ++--
 nextflow_schema.json | 4 ++--
 3 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 1a81afee..450ef11d 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -23,8 +23,8 @@ params {
 
     input            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/samplesheet/v2.0/samplesheet.csv'
     fasta            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.fa'
-    mature           = 'https://mirbase.org/download/CURRENT/mature.fa'
-    hairpin          = 'https://mirbase.org/download/CURRENT/hairpin.fa'
+    mature           = 'https://mirbase.org/download/mature.fa'
+    hairpin          = 'https://mirbase.org/download/hairpin.fa'
     mirna_gtf        = 'https://mirbase.org/download/hsa.gff3'
     mirtrace_species = 'hsa'
     protocol         = 'illumina'
diff --git a/nextflow.config b/nextflow.config
index 059feffe..f1a3dbcf 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -20,8 +20,8 @@ params {
     igenomes_base               = 's3://ngi-igenomes/igenomes'
     igenomes_ignore             = false
     mirna_gtf                   = null
-    mature                      = "https://mirbase.org/download/CURRENT/mature.fa"
-    hairpin                     = "https://mirbase.org/download/CURRENT/hairpin.fa"
+    mature                      = "https://mirbase.org/download/mature.fa"
+    hairpin                     = "https://mirbase.org/download/hairpin.fa"
     mirgenedb                   = false
     mirgenedb_mature            = null
     mirgenedb_hairpin           = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index d0162e47..cc061902 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -102,7 +102,7 @@
                     "description": "Path to FASTA file with mature miRNAs.",
                     "fa_icon": "fas fa-wheelchair",
                     "help_text": "Typically this will be the `mature.fa` file from miRBase. Can be given either as a plain text `.fa` file or a compressed `.gz` file.\n\nDefaults to the current miRBase release URL, from which the file will be downloaded.",
-                    "default": "https://mirbase.org/download/CURRENT/mature.fa"
+                    "default": "https://mirbase.org/download/mature.fa"
                 },
                 "mirgenedb_mature": {
                     "type": "string",
@@ -114,7 +114,7 @@
                     "description": "Path to FASTA file with miRNAs precursors.",
                     "fa_icon": "fab fa-cuttlefish",
                     "help_text": "Typically this will be the `mature.fa` file from miRBase. Can be given either as a plain text `.fa` file or a compressed `.gz` file.\n\nDefaults to the current miRBase release URL, from which the file will be downloaded.",
-                    "default": "https://mirbase.org/download/CURRENT/hairpin.fa"
+                    "default": "https://mirbase.org/download/hairpin.fa"
                 },
                 "mirgenedb_hairpin": {
                     "type": "string",

From 6948bad72d1355c3704d17f4a2f0df8a305078f0 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 08:39:16 +0100
Subject: [PATCH 031/227] detect bowtie2 idx instead of bowtie1 idx

---
 modules/local/bowtie_map_contaminants.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/bowtie_map_contaminants.nf b/modules/local/bowtie_map_contaminants.nf
index d10f13b5..e47ee7c7 100644
--- a/modules/local/bowtie_map_contaminants.nf
+++ b/modules/local/bowtie_map_contaminants.nf
@@ -22,7 +22,7 @@ process BOWTIE_MAP_CONTAMINANTS {
 
     script:
     """
-    INDEX=`find -L ./ -name "*.3.ebwt" | sed 's/.3.ebwt//'`
+    INDEX=`find -L ./ -name "*.3.bt2" | sed 's/.3.bt2//'`
     bowtie2 \\
         --threads ${task.cpus} \\
         --very-sensitive-local \\

From 16bb675f2bf2c5105cc9f956a8c6a93eea09523e Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 10:22:09 +0100
Subject: [PATCH 032/227] print mirQuant inputs

---
 workflows/smrnaseq.nf | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 049851d0..60c6106f 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -178,6 +178,11 @@ workflow SMRNASEQ {
 
     }
 
+    print reference_mature
+    print reference_hairpin
+    print mirna_gtf
+    print mirna_reads
+
     MIRNA_QUANT (
         [ [:], reference_mature],
         [ [:], reference_hairpin],

From 90527a2ab0bd1580d34f6ab39b643315746929d7 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 11:29:57 +0100
Subject: [PATCH 033/227] debug mirdeeprun

---
 subworkflows/local/mirdeep2.nf | 7 +++++++
 1 file changed, 7 insertions(+)

diff --git a/subworkflows/local/mirdeep2.nf b/subworkflows/local/mirdeep2.nf
index f8098ba5..484beabd 100644
--- a/subworkflows/local/mirdeep2.nf
+++ b/subworkflows/local/mirdeep2.nf
@@ -23,6 +23,13 @@ workflow MIRDEEP2 {
     MIRDEEP2_MAPPER ( MIRDEEP2_PIGZ.out.reads, index )
     ch_versions = ch_versions.mix(MIRDEEP2_MAPPER.out.versions.first())
 
+
+    MIRDEEP2_MAPPER.out.mirdeep2_inputs.view{ it -> "DEBUG: MIRDEEP2_MAPPER.out.mirdeep2_inputs: " }
+
+    print "DEBUG fasta:" + fasta
+    print "DEBUG hairpin:" + hairpin
+    print "DEBUG mature:" + mature
+
     MIRDEEP2_RUN ( fasta, MIRDEEP2_MAPPER.out.mirdeep2_inputs, hairpin, mature )
     ch_versions = ch_versions.mix(MIRDEEP2_RUN.out.versions.first())
 

From 39f0597536c3308fbbdef7c0f778ec4d61a772e4 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 11:32:12 +0100
Subject: [PATCH 034/227] remove previous debug print

---
 workflows/smrnaseq.nf | 5 -----
 1 file changed, 5 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 60c6106f..049851d0 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -178,11 +178,6 @@ workflow SMRNASEQ {
 
     }
 
-    print reference_mature
-    print reference_hairpin
-    print mirna_gtf
-    print mirna_reads
-
     MIRNA_QUANT (
         [ [:], reference_mature],
         [ [:], reference_hairpin],

From c198eda989caaccd3c86fc5131b0787161dda0ed Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 12:16:07 +0100
Subject: [PATCH 035/227] add more debug messages

---
 subworkflows/local/mirdeep2.nf | 6 +++---
 workflows/smrnaseq.nf          | 6 ++++++
 2 files changed, 9 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/mirdeep2.nf b/subworkflows/local/mirdeep2.nf
index 484beabd..e7ca4789 100644
--- a/subworkflows/local/mirdeep2.nf
+++ b/subworkflows/local/mirdeep2.nf
@@ -26,9 +26,9 @@ workflow MIRDEEP2 {
 
     MIRDEEP2_MAPPER.out.mirdeep2_inputs.view{ it -> "DEBUG: MIRDEEP2_MAPPER.out.mirdeep2_inputs: " }
 
-    print "DEBUG fasta:" + fasta
-    print "DEBUG hairpin:" + hairpin
-    print "DEBUG mature:" + mature
+    println "DEBUG fasta:" + fasta
+    println "DEBUG hairpin:" + hairpin
+    println "DEBUG mature:" + mature
 
     MIRDEEP2_RUN ( fasta, MIRDEEP2_MAPPER.out.mirdeep2_inputs, hairpin, mature )
     ch_versions = ch_versions.mix(MIRDEEP2_RUN.out.versions.first())
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 049851d0..a463de5a 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -195,6 +195,12 @@ workflow SMRNASEQ {
         genome_stats = GENOME_QUANT.out.stats
         ch_versions = ch_versions.mix(GENOME_QUANT.out.versions)
 
+        FASTQC_FASTP.out.reads.view { it -> println "DEBUG ==> FASTQC_FASTP.out.reads = $it" }
+        GENOME_QUANT.out.fasta.view { it -> println "DEBUG ==> GENOME_QUANT.out.fasta = $it" }
+        GENOME_QUANT.out.index.view { it -> println "DEBUG ==> GENOME_QUANT.out.index = $it" }
+        MIRNA_QUANT.out.fasta_hairpin.view { it -> println "DEBUG ==> MIRNA_QUANT.out.fasta_hairpin = $it" }
+        MIRNA_QUANT.out.fasta_mature.view { it -> println "DEBUG ==> MIRNA_QUANT.out.fasta_mature = $it" }
+
         if (!params.skip_mirdeep) {
             MIRDEEP2 (
                 FASTQC_FASTP.out.reads,

From 85cadb0d53e4eb422934d8e4f379a53a79f99822 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 12:22:15 +0100
Subject: [PATCH 036/227] remove prints

---
 subworkflows/local/mirdeep2.nf | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/subworkflows/local/mirdeep2.nf b/subworkflows/local/mirdeep2.nf
index e7ca4789..a08023a0 100644
--- a/subworkflows/local/mirdeep2.nf
+++ b/subworkflows/local/mirdeep2.nf
@@ -24,11 +24,11 @@ workflow MIRDEEP2 {
     ch_versions = ch_versions.mix(MIRDEEP2_MAPPER.out.versions.first())
 
 
-    MIRDEEP2_MAPPER.out.mirdeep2_inputs.view{ it -> "DEBUG: MIRDEEP2_MAPPER.out.mirdeep2_inputs: " }
+    // MIRDEEP2_MAPPER.out.mirdeep2_inputs.view{ it -> "DEBUG: MIRDEEP2_MAPPER.out.mirdeep2_inputs: " }
 
-    println "DEBUG fasta:" + fasta
-    println "DEBUG hairpin:" + hairpin
-    println "DEBUG mature:" + mature
+    // println "DEBUG fasta:" + fasta
+    // println "DEBUG hairpin:" + hairpin
+    // println "DEBUG mature:" + mature
 
     MIRDEEP2_RUN ( fasta, MIRDEEP2_MAPPER.out.mirdeep2_inputs, hairpin, mature )
     ch_versions = ch_versions.mix(MIRDEEP2_RUN.out.versions.first())

From 7c703c9b0a78781ed9d80fc5db42967e1376e7a3 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 12:34:32 +0100
Subject: [PATCH 037/227] remove metq from mature and hairpin

---
 workflows/smrnaseq.nf | 8 ++++++--
 1 file changed, 6 insertions(+), 2 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index a463de5a..be8ae374 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -195,11 +195,15 @@ workflow SMRNASEQ {
         genome_stats = GENOME_QUANT.out.stats
         ch_versions = ch_versions.mix(GENOME_QUANT.out.versions)
 
+        hairpin_clean = MIRNA_QUANT.out.fasta_hairpin.map { it -> it[1] }
+        mature_clean  = MIRNA_QUANT.out.fasta_mature.map { it -> it[1] }
         FASTQC_FASTP.out.reads.view { it -> println "DEBUG ==> FASTQC_FASTP.out.reads = $it" }
         GENOME_QUANT.out.fasta.view { it -> println "DEBUG ==> GENOME_QUANT.out.fasta = $it" }
         GENOME_QUANT.out.index.view { it -> println "DEBUG ==> GENOME_QUANT.out.index = $it" }
-        MIRNA_QUANT.out.fasta_hairpin.view { it -> println "DEBUG ==> MIRNA_QUANT.out.fasta_hairpin = $it" }
-        MIRNA_QUANT.out.fasta_mature.view { it -> println "DEBUG ==> MIRNA_QUANT.out.fasta_mature = $it" }
+        // MIRNA_QUANT.out.fasta_hairpin.view { it -> println "DEBUG ==> MIRNA_QUANT.out.fasta_hairpin = $it" }
+        // MIRNA_QUANT.out.fasta_mature.view { it -> println "DEBUG ==> MIRNA_QUANT.out.fasta_mature = $it" }
+        hairpin_clean.view { it -> println "DEBUG ==> hairpin_clean = $it" }
+        mature_clean.view { it -> println "DEBUG ==> mature_clean = $it" }
 
         if (!params.skip_mirdeep) {
             MIRDEEP2 (

From e8d04d21a4b6ce742389cfdf2fd73daca27523fb Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 12:37:32 +0100
Subject: [PATCH 038/227] connect clean hairpin / mature to mirdeep2

---
 workflows/smrnaseq.nf | 6 ++++--
 1 file changed, 4 insertions(+), 2 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index be8ae374..f4415d12 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -210,8 +210,10 @@ workflow SMRNASEQ {
                 FASTQC_FASTP.out.reads,
                 GENOME_QUANT.out.fasta,
                 GENOME_QUANT.out.index.collect(),
-                MIRNA_QUANT.out.fasta_hairpin,
-                MIRNA_QUANT.out.fasta_mature
+                // MIRNA_QUANT.out.fasta_hairpin,
+                // MIRNA_QUANT.out.fasta_mature
+                hairpin_clean,
+                mature_clean
             )
             ch_versions = ch_versions.mix(MIRDEEP2.out.versions)
         }

From 9285dfbe175e269f9dbd6e051802e3c537b2798c Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 12:41:06 +0100
Subject: [PATCH 039/227] remove meta from fasta in mirdeep2_run

---
 modules/local/mirdeep2_run.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/mirdeep2_run.nf b/modules/local/mirdeep2_run.nf
index e4e2aaaf..b676aa10 100644
--- a/modules/local/mirdeep2_run.nf
+++ b/modules/local/mirdeep2_run.nf
@@ -10,7 +10,7 @@ process MIRDEEP2_RUN {
         'biocontainers/mirdeep2:2.0.1.3--hdfd78af_1' }"
 
     input:
-    tuple val(meta2), path(fasta)
+    path(fasta)
     tuple path(reads), path(arf)
     path hairpin
     path mature

From 3a3f11bf057c9422d8e7eb40425b8e2c7f6ac704 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Mon, 16 Oct 2023 14:27:57 +0100
Subject: [PATCH 040/227] Clean debug prints

---
 subworkflows/local/mirdeep2.nf | 7 -------
 workflows/smrnaseq.nf          | 9 ---------
 2 files changed, 16 deletions(-)

diff --git a/subworkflows/local/mirdeep2.nf b/subworkflows/local/mirdeep2.nf
index a08023a0..f8098ba5 100644
--- a/subworkflows/local/mirdeep2.nf
+++ b/subworkflows/local/mirdeep2.nf
@@ -23,13 +23,6 @@ workflow MIRDEEP2 {
     MIRDEEP2_MAPPER ( MIRDEEP2_PIGZ.out.reads, index )
     ch_versions = ch_versions.mix(MIRDEEP2_MAPPER.out.versions.first())
 
-
-    // MIRDEEP2_MAPPER.out.mirdeep2_inputs.view{ it -> "DEBUG: MIRDEEP2_MAPPER.out.mirdeep2_inputs: " }
-
-    // println "DEBUG fasta:" + fasta
-    // println "DEBUG hairpin:" + hairpin
-    // println "DEBUG mature:" + mature
-
     MIRDEEP2_RUN ( fasta, MIRDEEP2_MAPPER.out.mirdeep2_inputs, hairpin, mature )
     ch_versions = ch_versions.mix(MIRDEEP2_RUN.out.versions.first())
 
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index f4415d12..17eb985d 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -197,21 +197,12 @@ workflow SMRNASEQ {
 
         hairpin_clean = MIRNA_QUANT.out.fasta_hairpin.map { it -> it[1] }
         mature_clean  = MIRNA_QUANT.out.fasta_mature.map { it -> it[1] }
-        FASTQC_FASTP.out.reads.view { it -> println "DEBUG ==> FASTQC_FASTP.out.reads = $it" }
-        GENOME_QUANT.out.fasta.view { it -> println "DEBUG ==> GENOME_QUANT.out.fasta = $it" }
-        GENOME_QUANT.out.index.view { it -> println "DEBUG ==> GENOME_QUANT.out.index = $it" }
-        // MIRNA_QUANT.out.fasta_hairpin.view { it -> println "DEBUG ==> MIRNA_QUANT.out.fasta_hairpin = $it" }
-        // MIRNA_QUANT.out.fasta_mature.view { it -> println "DEBUG ==> MIRNA_QUANT.out.fasta_mature = $it" }
-        hairpin_clean.view { it -> println "DEBUG ==> hairpin_clean = $it" }
-        mature_clean.view { it -> println "DEBUG ==> mature_clean = $it" }
 
         if (!params.skip_mirdeep) {
             MIRDEEP2 (
                 FASTQC_FASTP.out.reads,
                 GENOME_QUANT.out.fasta,
                 GENOME_QUANT.out.index.collect(),
-                // MIRNA_QUANT.out.fasta_hairpin,
-                // MIRNA_QUANT.out.fasta_mature
                 hairpin_clean,
                 mature_clean
             )

From c6efa86cf50dc2f359be18b309bea2bfe9590382 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Tue, 17 Oct 2023 09:30:51 +0100
Subject: [PATCH 041/227] update bowtie map contaminant with right options

---
 modules/local/bowtie_map_contaminants.nf | 11 ++++++-----
 1 file changed, 6 insertions(+), 5 deletions(-)

diff --git a/modules/local/bowtie_map_contaminants.nf b/modules/local/bowtie_map_contaminants.nf
index e47ee7c7..10f5dadd 100644
--- a/modules/local/bowtie_map_contaminants.nf
+++ b/modules/local/bowtie_map_contaminants.nf
@@ -22,16 +22,17 @@ process BOWTIE_MAP_CONTAMINANTS {
 
     script:
     """
-    INDEX=`find -L ./ -name "*.3.bt2" | sed 's/.3.bt2//'`
+    INDEX=`find -L ./ -name "*.rev.1.bt2" | sed "s/\\.rev.1.bt2\$//"`
     bowtie2 \\
+        -x \$INDEX \\
+        -U ${reads} \\
         --threads ${task.cpus} \\
+        --un ${meta.id}.${contaminant_type}.filter.unmapped.contaminant.fastq \\
         --very-sensitive-local \\
         -k 1 \\
-        -x \$INDEX \\
-        --un ${meta.id}.${contaminant_type}.filter.unmapped.contaminant.fastq \\
-        ${reads} \\
+        -S ${meta.id}.filter.contaminant.sam \\
         ${args} \\
-        -S ${meta.id}.filter.contaminant.sam > ${meta.id}.contaminant_bowtie.log 2>&1
+        > ${meta.id}.contaminant_bowtie.log 2>&1
 
     # extracting number of reads from bowtie logs
     awk -v type=${contaminant_type} 'BEGIN{tot=0} {if(NR==4 || NR == 5){tot += \$1}} END {print "\\""type"\\": "tot }' ${meta.id}.contaminant_bowtie.log | tr -d , > filtered.${meta.id}_${contaminant_type}.stats

From 0be5a172404e76da496418afc23e7ff0c061f301 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Tue, 17 Oct 2023 09:45:33 +0100
Subject: [PATCH 042/227] define args variable

---
 modules/local/bowtie_map_contaminants.nf | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/modules/local/bowtie_map_contaminants.nf b/modules/local/bowtie_map_contaminants.nf
index 10f5dadd..d744b1fd 100644
--- a/modules/local/bowtie_map_contaminants.nf
+++ b/modules/local/bowtie_map_contaminants.nf
@@ -21,6 +21,8 @@ process BOWTIE_MAP_CONTAMINANTS {
     task.ext.when == null || task.ext.when
 
     script:
+    def args = task.ext.args ?: ""
+
     """
     INDEX=`find -L ./ -name "*.rev.1.bt2" | sed "s/\\.rev.1.bt2\$//"`
     bowtie2 \\

From 13fc7a47725374e1dded9a9f42ff5815657ec033 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Tue, 17 Oct 2023 14:49:44 +0100
Subject: [PATCH 043/227] FIx? - include fastqc trim to multiQC

---
 workflows/smrnaseq.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 17eb985d..f73bc79c 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -230,7 +230,8 @@ workflow SMRNASEQ {
         ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
         ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQC_FASTP.out.fastqc_raw_zip.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files = ch_multiqc_files.mix(FASTQC_FASTP.out.trim_json.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQC_FASTP.out.fastqc_trim_zip.collect{it[1]}.ifEmpty([]))
+        // ch_multiqc_files = ch_multiqc_files.mix(FASTQC_FASTP.out.trim_json.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(contamination_stats.collect().ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(genome_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mature_stats.collect({it[1]}).ifEmpty([]))

From 71590dba904f39fa3d524f3eefee4c0df7d4f2f4 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Tue, 17 Oct 2023 15:32:04 +0100
Subject: [PATCH 044/227] Refresh pipeline summary

---
 README.md | 55 ++++++++++++++++++++++++++++++++-----------------------
 1 file changed, 32 insertions(+), 23 deletions(-)

diff --git a/README.md b/README.md
index 44066051..dea262d8 100644
--- a/README.md
+++ b/README.md
@@ -26,29 +26,38 @@ You can find numerous talks on the nf-core events page from various topics inclu
 
 ## Pipeline summary
 
-1. Raw read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-2. Adapter trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/))
-   1. Insert Size calculation
-   2. Collapse reads ([`seqcluster`](https://seqcluster.readthedocs.io/mirna_annotation.html#processing-of-reads))
-3. Contamination filtering ([`Bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml))
-4. Alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-5. Alignment against miRBase hairpin
-   1. Unaligned reads from step 3 ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-   2. Collapsed reads from step 2.2 ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-6. Post-alignment processing of miRBase hairpin
-   1. Basic statistics from step 3 and step 4.1 ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
-   2. Analysis on miRBase, or MirGeneDB hairpin counts ([`edgeR`](https://bioconductor.org/packages/release/bioc/html/edgeR.html))
-      - TMM normalization and a table of top expression hairpin
-      - MDS plot clustering samples
-      - Heatmap of sample similarities
-   3. miRNA and isomiR annotation from step 4.1 ([`mirtop`](https://github.com/miRTop/mirtop))
-7. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
-   1. Post-alignment processing of alignment against host reference genome ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
-8. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation))
-   1. Mapping against reference genome with the mapper module
-   2. Known and novel miRNA discovery with the mirdeep2 module
-9. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace))
-10. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
+1. Quality check and triming
+    1. Raw read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+    2. Adapter trimming ([`fastp`](https://github.com/OpenGene/fastp))
+    3. Trim read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+2. miRNA QC ([`miRTrace`](https://github.com/friedlanderlab/mirtrace))
+3. Contamination filtering ([`Bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) (Optional)
+    1. rRNA filtration
+    2. tRNA filtration
+    3. cDNA filtration
+    4. ncRNA filtration
+    5. piRNA filtration
+    6. Others filtration
+4. miRNA quantification
+    - EdgeR
+        1. Reads alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
+        2. Post-alignment processing of alignment against Mature miRNA ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
+        3. Unmapped reads (from reads vs mature miRNA) alignment against miRBase hairpin
+        4. Post-alignment processing of alignment against Hairpin ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
+        5. Analysis on miRBase, or MirGeneDB hairpin counts ([`edgeR`](https://bioconductor.org/packages/release/bioc/html/edgeR.html))
+            - TMM normalization and a table of top expression hairpin
+            - MDS plot clustering samples
+            - Heatmap of sample similarities
+    - Mirtop quantification
+        1. Read collapsing  ([`seqcluster`](https://github.com/lpantano/seqcluster))
+        2. miRNA and isomiR annotation ([`mirtop`](https://github.com/miRTop/mirtop))
+5. Genome Quantification (Optional)
+    1. Reads alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
+    2. Post-alignment processing of alignment against host reference genome ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
+6. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation)) (Optional)
+    1. Mapping against reference genome with the mapper module
+    2. Known and novel miRNA discovery with the mirdeep2 module
+7. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
 
 ## Usage
 

From 892ab8924217539ce33679b89eaffa1ff3e2fe30 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Tue, 17 Oct 2023 18:33:33 +0100
Subject: [PATCH 045/227] Fix version: missing quotes and misaligned END_VER

---
 modules/local/blat_mirna.nf          | 2 +-
 modules/local/mirdeep2_prepare.nf    | 2 +-
 modules/local/mirdeep2_run.nf        | 2 +-
 modules/local/mirtop_quant.nf        | 2 +-
 modules/local/mirtrace.nf            | 2 +-
 modules/local/parse_fasta_mirna.nf   | 2 +-
 modules/local/seqcluster_collapse.nf | 2 +-
 7 files changed, 7 insertions(+), 7 deletions(-)

diff --git a/modules/local/blat_mirna.nf b/modules/local/blat_mirna.nf
index 7f8a2324..aa0d3d51 100644
--- a/modules/local/blat_mirna.nf
+++ b/modules/local/blat_mirna.nf
@@ -53,7 +53,7 @@ process BLAT_MIRNA {
         blat -out=blast8 $mirna $contaminants /dev/stdout | awk 'BEGIN{FS="\t"}{if(\$11 < 1e-5)print \$1;}' | uniq > mirnahit.txt
         awk 'BEGIN { while((getline<"mirnahit.txt")>0) l[">"\$1]=1 } /^>/ {x = l[\$1]} {if(!x) print }' $contaminants  > filtered.fa
 
-cat <<-END_VERSIONS > versions.yml
+        cat <<-END_VERSIONS > versions.yml
         "${task.process}":
             blat: \$(echo \$(blat) | grep Standalone | awk '{ if (match(\$0,/[0-9]*[0-9]/,m)) print m[0] }')
         END_VERSIONS
diff --git a/modules/local/mirdeep2_prepare.nf b/modules/local/mirdeep2_prepare.nf
index 7e2f2437..124b5b63 100644
--- a/modules/local/mirdeep2_prepare.nf
+++ b/modules/local/mirdeep2_prepare.nf
@@ -23,7 +23,7 @@ process MIRDEEP2_PIGZ {
     pigz -f -d -p $task.cpus $reads
 
     cat <<-END_VERSIONS > versions.yml
-    ${task.process}":
+    "${task.process}":
         pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
     END_VERSIONS
     """
diff --git a/modules/local/mirdeep2_run.nf b/modules/local/mirdeep2_run.nf
index b676aa10..17a484c1 100644
--- a/modules/local/mirdeep2_run.nf
+++ b/modules/local/mirdeep2_run.nf
@@ -35,7 +35,7 @@ process MIRDEEP2_RUN {
         -z _${reads.simpleName}
 
     cat <<-END_VERSIONS > versions.yml
-    ${task.process}":
+    "${task.process}":
         mirdeep2: \$(echo "$VERSION")
     END_VERSIONS
     """
diff --git a/modules/local/mirtop_quant.nf b/modules/local/mirtop_quant.nf
index e97d6a09..ab38c93d 100644
--- a/modules/local/mirtop_quant.nf
+++ b/modules/local/mirtop_quant.nf
@@ -34,7 +34,7 @@ process MIRTOP_QUANT {
     mv mirtop/stats/mirtop_stats.log mirtop/stats/full_mirtop_stats.log
 
     cat <<-END_VERSIONS > versions.yml
-    ${task.process}":
+    "${task.process}":
         mirtop: \$(echo \$(mirtop --version 2>&1) | sed 's/^.*mirtop //')
     END_VERSIONS
     """
diff --git a/modules/local/mirtrace.nf b/modules/local/mirtrace.nf
index f576ebc0..95989293 100644
--- a/modules/local/mirtrace.nf
+++ b/modules/local/mirtrace.nf
@@ -43,7 +43,7 @@ process MIRTRACE_RUN {
         --force
 
     cat <<-END_VERSIONS > versions.yml
-    ${task.process}":
+    "${task.process}":
         mirtrace: \$(echo \$(mirtrace -v 2>&1))
     END_VERSIONS
     """
diff --git a/modules/local/parse_fasta_mirna.nf b/modules/local/parse_fasta_mirna.nf
index a0bbc75e..ad63401e 100644
--- a/modules/local/parse_fasta_mirna.nf
+++ b/modules/local/parse_fasta_mirna.nf
@@ -34,7 +34,7 @@ process PARSE_FASTA_MIRNA {
     seqkit seq --rna2dna \${FASTA}_sps.fa > \${FASTA}_igenome.fa
 
     cat <<-END_VERSIONS > versions.yml
-    ${task.process}":
+    "${task.process}":
         seqkit: \$(echo \$(seqkit 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
     END_VERSIONS
     """
diff --git a/modules/local/seqcluster_collapse.nf b/modules/local/seqcluster_collapse.nf
index 39f6ce85..82470e5a 100644
--- a/modules/local/seqcluster_collapse.nf
+++ b/modules/local/seqcluster_collapse.nf
@@ -25,7 +25,7 @@ process SEQCLUSTER_SEQUENCES {
     mv collapsed/*.fastq.gz final/.
 
     cat <<-END_VERSIONS > versions.yml
-    ${task.process}":
+    "${task.process}":
         seqcluster: \$(echo \$(seqcluster --version 2>&1) | sed 's/^.*seqcluster //')
     END_VERSIONS
     """

From 05ae65ad2f2e9ad9c1117f55697298266389a0f9 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Tue, 17 Oct 2023 18:34:03 +0100
Subject: [PATCH 046/227] Update doc with HTML less fa

---
 docs/usage.md | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 9bfe5494..b0b7e647 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -27,10 +27,10 @@ It should point to the 3-letter species name used by [miRBase](https://www.mirba
 Different parameters can be set for the two supported databases. By default `miRBase` will be used with the parameters below.
 
 - `mirna_gtf`: If not supplied by the user, then `mirna_gtf` will point to the latest GFF3 file in miRbase: `https://mirbase.org/download/CURRENT/genomes/${params.mirtrace_species}.gff3`
-- `mature`: points to the FASTA file of mature miRNA sequences. `https://mirbase.org/download/CURRENT/mature.fa`
-- `hairpin`: points to the FASTA file of precursor miRNA sequences. `https://mirbase.org/download/CURRENT/hairpin.fa`
+- `mature`: points to the FASTA file of mature miRNA sequences. `https://mirbase.org/download/mature.fa`
+- `hairpin`: points to the FASTA file of precursor miRNA sequences. `https://mirbase.org/download/hairpin.fa`
 
-If MirGeneDB should be used instead it needs to be specified using `--mirgenedb` and use the parameters below .
+If MirGeneDB should be used instead it needs to be specified using `--mirgenedb` and use the parameters below.
 
 - `mirgenedb_gff`: The data can not be downloaded automatically (URLs are created with short term tokens in it), thus the user needs to supply the gff file for either his species, or all species downloaded from `https://mirgenedb.org/download`. The total set will automatically be subsetted to the species specified with `--mirgenedb_species`.
 - `mirgenedb_mature`: points to the FASTA file of mature miRNA sequences. Download from `https://mirgenedb.org/download`.

From e6a0b75b4ae05dc2d1203265b3ea53a8c883b203 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Wed, 18 Oct 2023 08:18:57 +0100
Subject: [PATCH 047/227] update mirtrace ouput directory

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index 556d8509..ba33f736 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -80,7 +80,7 @@ process {
 process {
     withName: 'MIRTRACE_RUN' {
         publishDir = [
-            path: { "${params.outdir}/mirtrace/${meta.id}" },
+            path: { "${params.outdir}/mirtrace" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]

From dd979483a127713a273590c93ed4c706bbfcc187 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Wed, 18 Oct 2023 10:05:37 +0100
Subject: [PATCH 048/227] update fastqc config

---
 conf/modules.config | 16 ++++++++--------
 1 file changed, 8 insertions(+), 8 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index ba33f736..8620836e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -87,14 +87,6 @@ process {
     }
 }
 
-if (!(params.skip_fastqc)) {
-    process {
-        withName: '.*:FASTQC_FASTP:FASTQC_.*' {
-            ext.args = '--quiet'
-        }
-    }
-}
-
 if (!params.skip_fastp) {
     process {
         withName: 'FASTP' {
@@ -130,6 +122,14 @@ if (!params.skip_fastp) {
 
     if (!params.skip_fastqc) {
         process {
+            withName: '.*:.*:FASTQC_FASTP:FASTQC_RAW' {
+                ext.args = '--quiet'
+                publishDir = [
+                    path: { "${params.outdir}/fastqc/raw" },
+                    mode: params.publish_dir_mode,
+                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+                ]
+            }
             withName: '.*:.*:FASTQC_FASTP:FASTQC_TRIM' {
                 ext.args = '--quiet'
                 publishDir = [

From b59a73f317442b7dceb6f8e2772a6bb0240eaa98 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?S=C3=A9bastien=20Guizard?= <sguizard@ed.ac.uk>
Date: Wed, 18 Oct 2023 10:57:08 +0100
Subject: [PATCH 049/227] Update mirdeep2 publish_dir path

---
 conf/modules.config | 9 ++++++++-
 1 file changed, 8 insertions(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index 8620836e..21b16110 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -236,7 +236,14 @@ if (!params.skip_mirdeep) {
     process {
         withName: 'MIRDEEP2_MAPPER' {
             publishDir = [
-                path: { "${params.outdir}/mirdeep" },
+                path: { "${params.outdir}/mirdeep2/mapper" },
+                mode: params.publish_dir_mode,
+                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            ]
+        }
+        withName: 'MIRDEEP2_RUN' {
+            publishDir = [
+                path: { "${params.outdir}/mirdeep2/run" },
                 mode: params.publish_dir_mode,
                 saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
             ]

From cdeb10ce4afd8050fb829ffa547300d47be3dcfa Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 3 Nov 2023 19:33:09 +0000
Subject: [PATCH 050/227] BUmp dev

---
 CHANGELOG.md              | 4 ++++
 assets/multiqc_config.yml | 4 ++--
 nextflow.config           | 2 +-
 3 files changed, 7 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 97613a1c..47cb7b72 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,6 +3,10 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## [dev](https://github.com/nf-core/smrnaseq/branch/dev)
+
+- _nothing yet done_
+
 ## [v2.2.4](https://github.com/nf-core/smrnaseq/releases/tag/2.2.4) - 2023-11-03
 
 - Update template to 2.10
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 435afefa..74c7de72 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,7 +1,7 @@
 report_comment: >
-  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/releases/tag/2.2.4" target="_blank">nf-core/smrnaseq</a>
+  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/releases/tag/dev" target="_blank">nf-core/smrnaseq</a>
   analysis pipeline. For information about how to interpret these results, please see the
-  <a href="https://nf-co.re/smrnaseq/2.2.4/docs/output" target="_blank">documentation</a>.
+  <a href="https://nf-co.re/smrnaseq/dev/docs/output" target="_blank">documentation</a>.
 report_section_order:
   "nf-core-smrnaseq-methods-description":
     order: -1000
diff --git a/nextflow.config b/nextflow.config
index 2fb69a90..2038c633 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -287,7 +287,7 @@ manifest {
     description     = """Small RNA-Seq Best Practice Analysis Pipeline."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version = '2.2.4'
+    version = '2.3dev'
     doi             = '10.5281/zenodo.3456879'
 }
 

From c835f592a7583222f77c9d23f9edf81c1067791e Mon Sep 17 00:00:00 2001
From: Fabian Hausmann <fabian.hausmann@zmnh.uni-hamburg.de>
Date: Tue, 28 Nov 2023 09:55:00 +0100
Subject: [PATCH 051/227] Fix stats not executed from genome

---
 subworkflows/local/genome_quant.nf | 8 +++++---
 1 file changed, 5 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/genome_quant.nf b/subworkflows/local/genome_quant.nf
index 967b2757..b310c76a 100644
--- a/subworkflows/local/genome_quant.nf
+++ b/subworkflows/local/genome_quant.nf
@@ -8,7 +8,7 @@ include { BOWTIE_MAP_SEQ as BOWTIE_MAP_GENOME } from '../../modules/local/bowtie
 
 workflow GENOME_QUANT {
     take:
-    fasta 
+    fasta
     index
     reads // channel: [ val(meta), [ reads ] ]
 
@@ -28,8 +28,10 @@ workflow GENOME_QUANT {
     if (bowtie_index){
         BOWTIE_MAP_GENOME ( reads, bowtie_index.collect() )
         ch_versions = ch_versions.mix(BOWTIE_MAP_GENOME.out.versions)
-
-        BAM_SORT_STATS_SAMTOOLS ( BOWTIE_MAP_GENOME.out.bam, Channel.empty()  )
+        fasta_formatted
+            .map { file -> tuple(file.baseName, file) }
+            .set { sort_input }
+        BAM_SORT_STATS_SAMTOOLS ( BOWTIE_MAP_GENOME.out.bam,  sort_input )
         ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions)
     }
 

From 7278013731567b2d516e9046438233c8f818c971 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 19 Dec 2023 18:36:51 +0000
Subject: [PATCH 052/227] Template update for nf-core/tools version 2.11

---
 .github/CONTRIBUTING.md                       |   3 +
 .github/PULL_REQUEST_TEMPLATE.md              |   1 +
 .github/workflows/ci.yml                      |   2 +-
 .github/workflows/fix-linting.yml             |   4 +-
 .github/workflows/linting.yml                 |  12 +-
 .gitpod.yml                                   |   4 +-
 CHANGELOG.md                                  |   2 +-
 README.md                                     |  17 +--
 assets/multiqc_config.yml                     |   6 +-
 assets/slackreport.json                       |   2 +-
 conf/modules.config                           |   2 +-
 docs/usage.md                                 |   4 +-
 lib/NfcoreTemplate.groovy                     |  32 ++---
 modules.json                                  |   6 +-
 .../dumpsoftwareversions/environment.yml      |   7 ++
 .../custom/dumpsoftwareversions/main.nf       |   6 +-
 .../custom/dumpsoftwareversions/meta.yml      |   7 +-
 .../dumpsoftwareversions/tests/main.nf.test   |  38 ++++++
 .../tests/main.nf.test.snap                   |  27 +++++
 .../dumpsoftwareversions/tests/tags.yml       |   2 +
 modules/nf-core/fastqc/environment.yml        |   7 ++
 modules/nf-core/fastqc/main.nf                |  10 +-
 modules/nf-core/fastqc/meta.yml               |   5 +
 modules/nf-core/fastqc/tests/main.nf.test     | 109 ++++++++++++++++++
 .../nf-core/fastqc/tests/main.nf.test.snap    |  10 ++
 modules/nf-core/fastqc/tests/tags.yml         |   2 +
 modules/nf-core/multiqc/environment.yml       |   7 ++
 modules/nf-core/multiqc/main.nf               |   8 +-
 modules/nf-core/multiqc/meta.yml              |  11 +-
 modules/nf-core/multiqc/tests/main.nf.test    |  63 ++++++++++
 modules/nf-core/multiqc/tests/tags.yml        |   2 +
 nextflow.config                               |  12 +-
 32 files changed, 361 insertions(+), 69 deletions(-)
 create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml
 create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
 create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
 create mode 100644 modules/nf-core/fastqc/environment.yml
 create mode 100644 modules/nf-core/fastqc/tests/main.nf.test
 create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/fastqc/tests/tags.yml
 create mode 100644 modules/nf-core/multiqc/environment.yml
 create mode 100644 modules/nf-core/multiqc/tests/main.nf.test
 create mode 100644 modules/nf-core/multiqc/tests/tags.yml

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 4d09370b..bd134c88 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -27,6 +27,9 @@ If you're not used to this workflow with git, you can start with some [docs from
 
 ## Tests
 
+You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to
+receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir <OUTDIR>`.
+
 When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests.
 Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then.
 
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index e278390b..3cdbf2b7 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -19,6 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/smrn
 - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
+- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
 - [ ] `CHANGELOG.md` is updated.
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index d40ffd75..60f93b48 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -28,7 +28,7 @@ jobs:
           - "latest-everything"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v3
+        uses: actions/checkout@v4
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v1
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index ae6a0d7b..0cd44ed6 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -13,7 +13,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
@@ -24,7 +24,7 @@ jobs:
         env:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
-      - uses: actions/setup-node@v3
+      - uses: actions/setup-node@v4
 
       - name: Install Prettier
         run: npm install -g prettier @prettier/plugin-php
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index b8bdd214..905c58e4 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,9 +14,9 @@ jobs:
   EditorConfig:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
 
-      - uses: actions/setup-node@v3
+      - uses: actions/setup-node@v4
 
       - name: Install editorconfig-checker
         run: npm install -g editorconfig-checker
@@ -27,9 +27,9 @@ jobs:
   Prettier:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
 
-      - uses: actions/setup-node@v3
+      - uses: actions/setup-node@v4
 
       - name: Install Prettier
         run: npm install -g prettier
@@ -40,7 +40,7 @@ jobs:
   PythonBlack:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
 
       - name: Check code lints with Black
         uses: psf/black@stable
@@ -71,7 +71,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v3
+        uses: actions/checkout@v4
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v1
diff --git a/.gitpod.yml b/.gitpod.yml
index 25488dcc..acf72695 100644
--- a/.gitpod.yml
+++ b/.gitpod.yml
@@ -4,7 +4,9 @@ tasks:
     command: |
       pre-commit install --install-hooks
       nextflow self-update
-
+  - name: unset JAVA_TOOL_OPTIONS
+    command: |
+      unset JAVA_TOOL_OPTIONS
 vscode:
   extensions: # based on nf-core.nf-core-extensionpack
     - codezombiech.gitignore # Language support for .gitignore files
diff --git a/CHANGELOG.md b/CHANGELOG.md
index d6c4fb89..809d1ec2 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.2.3 - [date]
+## v2.3dev - [date]
 
 Initial release of nf-core/smrnaseq, created with the [nf-core](https://nf-co.re/) template.
 
diff --git a/README.md b/README.md
index e5dc1d80..d0b5ad21 100644
--- a/README.md
+++ b/README.md
@@ -30,11 +30,8 @@
 
 ## Usage
 
-:::note
-If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how
-to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline)
-with `-profile test` before running the workflow on actual data.
-:::
+> [!NOTE]
+> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.
 
 <!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.
      Explain what rows and columns represent. For instance (please edit as appropriate):
@@ -63,11 +60,9 @@ nextflow run nf-core/smrnaseq \
    --outdir <OUTDIR>
 ```
 
-:::warning
-Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those
-provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_;
-see [docs](https://nf-co.re/usage/configuration#custom-configuration-files).
-:::
+> [!WARNING]
+> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_;
+> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files).
 
 For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/smrnaseq/usage) and the [parameter documentation](https://nf-co.re/smrnaseq/parameters).
 
@@ -94,7 +89,7 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 ## Citations
 
 <!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->
-<!-- If you use  nf-core/smrnaseq for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
+<!-- If you use nf-core/smrnaseq for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
 
 <!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
 
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 267c3cc9..b92cb9e7 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,9 +1,7 @@
 report_comment: >
-  
-  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/releases/tag/2.2.3" target="_blank">nf-core/smrnaseq</a>
+  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/tree/dev" target="_blank">nf-core/smrnaseq</a>
   analysis pipeline. For information about how to interpret these results, please see the
-  <a href="https://nf-co.re/smrnaseq/2.2.3/docs/output" target="_blank">documentation</a>.
-  
+  <a href="https://nf-co.re/smrnaseq/dev/docs/output" target="_blank">documentation</a>.
 report_section_order:
   "nf-core-smrnaseq-methods-description":
     order: -1000
diff --git a/assets/slackreport.json b/assets/slackreport.json
index 214c7fa9..1884b21a 100644
--- a/assets/slackreport.json
+++ b/assets/slackreport.json
@@ -3,7 +3,7 @@
         {
             "fallback": "Plain-text summary of the attachment.",
             "color": "<% if (success) { %>good<% } else { %>danger<%} %>",
-            "author_name": "nf-core/smrnaseq v${version} - ${runName}",
+            "author_name": "nf-core/smrnaseq ${version} - ${runName}",
             "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico",
             "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>",
             "fields": [
diff --git a/conf/modules.config b/conf/modules.config
index 39e81386..d91c6aba 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -39,7 +39,7 @@ process {
     }
 
     withName: 'MULTIQC' {
-        ext.args   = params.multiqc_title ? "--title \"$params.multiqc_title\"" : ''
+        ext.args   = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' }
         publishDir = [
             path: { "${params.outdir}/multiqc" },
             mode: params.publish_dir_mode,
diff --git a/docs/usage.md b/docs/usage.md
index 8878af27..f14fb92e 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -20,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou
 
 The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes:
 
-```console
+```csv title="samplesheet.csv"
 sample,fastq_1,fastq_2
 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
 CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz
@@ -33,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th
 
 A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice.
 
-```console
+```csv title="samplesheet.csv"
 sample,fastq_1,fastq_2
 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
 CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index 01b8653d..e248e4c3 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -4,6 +4,7 @@
 
 import org.yaml.snakeyaml.Yaml
 import groovy.json.JsonOutput
+import nextflow.extension.FilesEx
 
 class NfcoreTemplate {
 
@@ -141,12 +142,14 @@ class NfcoreTemplate {
             try {
                 if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
                 // Try to send HTML e-mail using sendmail
+                def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
+                sendmail_tf.withWriter { w -> w << sendmail_html }
                 [ 'sendmail', '-t' ].execute() << sendmail_html
                 log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
             } catch (all) {
                 // Catch failures and try with plaintext
                 def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
-                if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
+                if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
                     mail_cmd += [ '-A', mqc_report ]
                 }
                 mail_cmd.execute() << email_html
@@ -155,14 +158,16 @@ class NfcoreTemplate {
         }
 
         // Write summary e-mail HTML to a file
-        def output_d = new File("${params.outdir}/pipeline_info/")
-        if (!output_d.exists()) {
-            output_d.mkdirs()
-        }
-        def output_hf = new File(output_d, "pipeline_report.html")
+        def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
         output_hf.withWriter { w -> w << email_html }
-        def output_tf = new File(output_d, "pipeline_report.txt")
+        FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html");
+        output_hf.delete()
+
+        // Write summary e-mail TXT to a file
+        def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
         output_tf.withWriter { w -> w << email_txt }
+        FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt");
+        output_tf.delete()
     }
 
     //
@@ -227,15 +232,14 @@ class NfcoreTemplate {
     // Dump pipeline parameters in a json file
     //
     public static void dump_parameters(workflow, params) {
-        def output_d = new File("${params.outdir}/pipeline_info/")
-        if (!output_d.exists()) {
-            output_d.mkdirs()
-        }
-
         def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
-        def output_pf  = new File(output_d, "params_${timestamp}.json")
+        def filename   = "params_${timestamp}.json"
+        def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
         def jsonStr    = JsonOutput.toJson(params)
-        output_pf.text = JsonOutput.prettyPrint(jsonStr)
+        temp_pf.text   = JsonOutput.prettyPrint(jsonStr)
+
+        FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json")
+        temp_pf.delete()
     }
 
     //
diff --git a/modules.json b/modules.json
index 98249a7b..0606570e 100644
--- a/modules.json
+++ b/modules.json
@@ -7,17 +7,17 @@
                 "nf-core": {
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "bd8092b67b5103bdd52e300f75889442275c3117",
+                        "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "4ab13872435962dadc239979554d13709e20bf29",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
new file mode 100644
index 00000000..f0c63f69
--- /dev/null
+++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
@@ -0,0 +1,7 @@
+name: custom_dumpsoftwareversions
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::multiqc=1.17
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index ebc87273..7685b33c 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "bioconda::multiqc=1.14"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.14--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.17--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
index c32657de..5f15a5fd 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
@@ -1,4 +1,4 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
 name: custom_dumpsoftwareversions
 description: Custom module used to dump software versions within the nf-core pipeline template
 keywords:
@@ -16,7 +16,6 @@ input:
       type: file
       description: YML file containing software versions
       pattern: "*.yml"
-
 output:
   - yml:
       type: file
@@ -30,7 +29,9 @@ output:
       type: file
       description: File containing software versions
       pattern: "versions.yml"
-
 authors:
   - "@drpatelh"
   - "@grst"
+maintainers:
+  - "@drpatelh"
+  - "@grst"
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
new file mode 100644
index 00000000..eec1db10
--- /dev/null
+++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
@@ -0,0 +1,38 @@
+nextflow_process {
+
+    name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS"
+    script "../main.nf"
+    process "CUSTOM_DUMPSOFTWAREVERSIONS"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "custom"
+    tag "dumpsoftwareversions"
+    tag "custom/dumpsoftwareversions"
+
+    test("Should run without failures") {
+        when {
+            process {
+                """
+                def tool1_version = '''
+                TOOL1:
+                    tool1: 0.11.9
+                '''.stripIndent()
+
+                def tool2_version = '''
+                TOOL2:
+                    tool2: 1.9
+                '''.stripIndent()
+
+                input[0] = Channel.of(tool1_version, tool2_version).collectFile()
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
new file mode 100644
index 00000000..4274ed57
--- /dev/null
+++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
@@ -0,0 +1,27 @@
+{
+    "Should run without failures": {
+        "content": [
+            {
+                "0": [
+                    "software_versions.yml:md5,1c851188476409cda5752ce971b20b58"
+                ],
+                "1": [
+                    "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d"
+                ],
+                "2": [
+                    "versions.yml:md5,3843ac526e762117eedf8825b40683df"
+                ],
+                "mqc_yml": [
+                    "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d"
+                ],
+                "versions": [
+                    "versions.yml:md5,3843ac526e762117eedf8825b40683df"
+                ],
+                "yml": [
+                    "software_versions.yml:md5,1c851188476409cda5752ce971b20b58"
+                ]
+            }
+        ],
+        "timestamp": "2023-11-03T14:43:22.157011"
+    }
+}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
new file mode 100644
index 00000000..405aa24a
--- /dev/null
+++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
@@ -0,0 +1,2 @@
+custom/dumpsoftwareversions:
+  - modules/nf-core/custom/dumpsoftwareversions/**
diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml
new file mode 100644
index 00000000..1787b38a
--- /dev/null
+++ b/modules/nf-core/fastqc/environment.yml
@@ -0,0 +1,7 @@
+name: fastqc
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::fastqc=0.12.1
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 249f9064..9e19a74c 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -2,10 +2,10 @@ process FASTQC {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::fastqc=0.11.9"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' :
-        'biocontainers/fastqc:0.11.9--0' }"
+        'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' :
+        'biocontainers/fastqc:0.12.1--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(reads)
@@ -37,7 +37,7 @@ process FASTQC {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' )
     END_VERSIONS
     """
 
@@ -49,7 +49,7 @@ process FASTQC {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' )
     END_VERSIONS
     """
 }
diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml
index 4da5bb5a..ee5507e0 100644
--- a/modules/nf-core/fastqc/meta.yml
+++ b/modules/nf-core/fastqc/meta.yml
@@ -50,3 +50,8 @@ authors:
   - "@grst"
   - "@ewels"
   - "@FelixKrueger"
+maintainers:
+  - "@drpatelh"
+  - "@grst"
+  - "@ewels"
+  - "@FelixKrueger"
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
new file mode 100644
index 00000000..b9e8f926
--- /dev/null
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -0,0 +1,109 @@
+nextflow_process {
+
+    name "Test Process FASTQC"
+    script "../main.nf"
+    process "FASTQC"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "fastqc"
+
+    test("Single-Read") {
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id: 'test', single_end:true ],
+                    [
+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                    ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
+            // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
+            // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
+            { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" },
+            { assert path(process.out.html.get(0).get(1)).getText().contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert snapshot(process.out.versions).match("versions") },
+            { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" }
+            )
+        }
+    }
+// TODO
+// //
+// // Test with paired-end data
+// //
+// workflow test_fastqc_paired_end {
+//     input = [
+//                 [id: 'test', single_end: false], // meta map
+//                 [
+//                     file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+//                     file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+//                 ]
+//             ]
+
+//     FASTQC ( input )
+// }
+
+// //
+// // Test with interleaved data
+// //
+// workflow test_fastqc_interleaved {
+//     input = [
+//                 [id: 'test', single_end: false], // meta map
+//                 file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true)
+//             ]
+
+//     FASTQC ( input )
+// }
+
+// //
+// // Test with bam data
+// //
+// workflow test_fastqc_bam {
+//     input = [
+//                 [id: 'test', single_end: false], // meta map
+//                 file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
+//             ]
+
+//     FASTQC ( input )
+// }
+
+// //
+// // Test with multiple samples
+// //
+// workflow test_fastqc_multiple {
+//     input = [
+//                 [id: 'test', single_end: false], // meta map
+//                 [
+//                     file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+//                     file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true),
+//                     file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true),
+//                     file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true)
+//                 ]
+//             ]
+
+//     FASTQC ( input )
+// }
+
+// //
+// // Test with custom prefix
+// //
+// workflow test_fastqc_custom_prefix {
+//     input = [
+//                 [ id:'mysample', single_end:true ], // meta map
+//                 file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+//             ]
+
+//     FASTQC ( input )
+// }
+}
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
new file mode 100644
index 00000000..636a32ce
--- /dev/null
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -0,0 +1,10 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "timestamp": "2023-10-09T23:40:54+0000"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml
new file mode 100644
index 00000000..7834294b
--- /dev/null
+++ b/modules/nf-core/fastqc/tests/tags.yml
@@ -0,0 +1,2 @@
+fastqc:
+  - modules/nf-core/fastqc/**
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
new file mode 100644
index 00000000..bc0bdb5b
--- /dev/null
+++ b/modules/nf-core/multiqc/environment.yml
@@ -0,0 +1,7 @@
+name: multiqc
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::multiqc=1.18
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 1fc387be..00cc48d2 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -1,10 +1,10 @@
 process MULTIQC {
     label 'process_single'
 
-    conda "bioconda::multiqc=1.14"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.14--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.18--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
@@ -25,12 +25,14 @@ process MULTIQC {
     def args = task.ext.args ?: ''
     def config = multiqc_config ? "--config $multiqc_config" : ''
     def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
+    def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : ''
     """
     multiqc \\
         --force \\
         $args \\
         $config \\
         $extra_config \\
+        $logo \\
         .
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index f93b5ee5..f1aa660e 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,5 +1,5 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
-name: MultiQC
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: multiqc
 description: Aggregate results from bioinformatics analyses across many samples into a single report
 keywords:
   - QC
@@ -13,7 +13,6 @@ tools:
       homepage: https://multiqc.info/
       documentation: https://multiqc.info/docs/
       licence: ["GPL-3.0-or-later"]
-
 input:
   - multiqc_files:
       type: file
@@ -31,7 +30,6 @@ input:
       type: file
       description: Optional logo file for MultiQC
       pattern: "*.{png}"
-
 output:
   - report:
       type: file
@@ -54,3 +52,8 @@ authors:
   - "@bunop"
   - "@drpatelh"
   - "@jfy133"
+maintainers:
+  - "@abhi18av"
+  - "@bunop"
+  - "@drpatelh"
+  - "@jfy133"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
new file mode 100644
index 00000000..c2dad217
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -0,0 +1,63 @@
+nextflow_process {
+
+    name "Test Process MULTIQC"
+    script "../main.nf"
+    process "MULTIQC"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "multiqc"
+
+    test("MULTIQC: FASTQC") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.report.get(0)).exists() },
+                { assert path(process.out.data.get(0)).exists() },
+                { assert path(process.out.versions.get(0)).getText().contains("multiqc") }
+            )
+        }
+
+    }
+
+    test("MULTIQC: FASTQC and a config file") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.report.get(0)).exists() },
+                { assert path(process.out.data.get(0)).exists() },
+                { assert path(process.out.versions.get(0)).getText().contains("multiqc") }
+            )
+        }
+
+    }
+}
diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml
new file mode 100644
index 00000000..bea6c0d3
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/tags.yml
@@ -0,0 +1,2 @@
+multiqc:
+  - modules/nf-core/multiqc/**
diff --git a/nextflow.config b/nextflow.config
index 79a64b2a..b01c59fa 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -14,7 +14,7 @@ params {
     input                      = null
     // References
     genome                     = null
-    igenomes_base              = 's3://ngi-igenomes/igenomes'
+    igenomes_base              = 's3://ngi-igenomes/igenomes/'
     igenomes_ignore            = false
     
 
@@ -82,6 +82,7 @@ profiles {
         dumpHashes             = true
         process.beforeScript   = 'echo $HOSTNAME'
         cleanup                = false
+        nextflow.enable.configProcessNamesValidation = true
     }
     conda {
         conda.enabled          = true
@@ -104,13 +105,13 @@ profiles {
     }
     docker {
         docker.enabled         = true
-        docker.userEmulation   = true
         conda.enabled          = false
         singularity.enabled    = false
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
         apptainer.enabled      = false
+        runOptions             = '-u $(id -u):$(id -g)'
     }
     arm {
         docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64'
@@ -181,7 +182,7 @@ singularity.registry = 'quay.io'
 
 // Nextflow plugins
 plugins {
-    id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet
+    id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet
 }
 
 // Load igenomes.config if required
@@ -204,6 +205,9 @@ env {
 // Capture exit codes from upstream processes when piping
 process.shell = ['/bin/bash', '-euo', 'pipefail']
 
+// Disable process selector warnings by default. Use debug profile to enable warnings.
+nextflow.enable.configProcessNamesValidation = false
+
 def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
 timeline {
     enabled = true
@@ -229,7 +233,7 @@ manifest {
     description     = """Small RNA-Seq Best Practice Analysis Pipeline."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '2.2.3'
+    version         = '2.3dev'
     doi             = ''
 }
 

From ec3d44615456f2ce58094c587b4353c502072d6c Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Wed, 20 Dec 2023 17:05:35 +0000
Subject: [PATCH 053/227] Template update for nf-core/tools version 2.11.1

---
 .../{release-announcments.yml => release-announcements.yml}   | 0
 nextflow.config                                               | 4 ++--
 2 files changed, 2 insertions(+), 2 deletions(-)
 rename .github/workflows/{release-announcments.yml => release-announcements.yml} (100%)

diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcements.yml
similarity index 100%
rename from .github/workflows/release-announcments.yml
rename to .github/workflows/release-announcements.yml
diff --git a/nextflow.config b/nextflow.config
index b01c59fa..a473a3b6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -111,10 +111,10 @@ profiles {
         shifter.enabled        = false
         charliecloud.enabled   = false
         apptainer.enabled      = false
-        runOptions             = '-u $(id -u):$(id -g)'
+        docker.runOptions      = '-u $(id -u):$(id -g)'
     }
     arm {
-        docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64'
+        docker.runOptions      = '-u $(id -u):$(id -g) --platform=linux/amd64'
     }
     singularity {
         singularity.enabled    = true

From ce28d5a06fdf9e891c51d717893b4869f3b44a4d Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 11 Jan 2024 13:01:04 +0000
Subject: [PATCH 054/227] First batch of updates to 2024

---
 modules.json                                  |  33 ++-
 modules/nf-core/fastp/environment.yml         |   1 +
 modules/nf-core/fastp/main.nf                 |  26 +-
 modules/nf-core/fastp/tests/main.nf.test      | 241 ++++++++++++++++++
 modules/nf-core/fastp/tests/main.nf.test.snap |  55 ++++
 modules/nf-core/modules/cat/cat/main.nf       |  62 -----
 modules/nf-core/modules/cat/cat/meta.yml      |  37 ---
 .../nf-core/modules/samtools/bam2fq/main.nf   |  56 ----
 .../nf-core/modules/samtools/bam2fq/meta.yml  |  55 ----
 .../nf-core/modules/umitools/dedup/main.nf    |  41 ---
 .../nf-core/modules/umitools/dedup/meta.yml   |  59 -----
 .../nf-core/modules/umitools/extract/main.nf  |  55 ----
 .../nf-core/modules/umitools/extract/meta.yml |  47 ----
 modules/nf-core/multiqc/environment.yml       |   3 +-
 modules/nf-core/multiqc/main.nf               |  10 +-
 modules/nf-core/multiqc/meta.yml              |   3 +-
 16 files changed, 357 insertions(+), 427 deletions(-)
 delete mode 100644 modules/nf-core/modules/cat/cat/main.nf
 delete mode 100644 modules/nf-core/modules/cat/cat/meta.yml
 delete mode 100644 modules/nf-core/modules/samtools/bam2fq/main.nf
 delete mode 100644 modules/nf-core/modules/samtools/bam2fq/meta.yml
 delete mode 100644 modules/nf-core/modules/umitools/dedup/main.nf
 delete mode 100644 modules/nf-core/modules/umitools/dedup/meta.yml
 delete mode 100644 modules/nf-core/modules/umitools/extract/main.nf
 delete mode 100644 modules/nf-core/modules/umitools/extract/meta.yml

diff --git a/modules.json b/modules.json
index 1250670b..4a236584 100644
--- a/modules.json
+++ b/modules.json
@@ -5,6 +5,11 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
+                    "cat/cat": {
+                        "branch": "master",
+                        "git_sha": "d593e8f6b7d1bbbb2acf43a4b9efeeac8d6720f2",
+                        "installed_by": ["modules"]
+                    },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
@@ -17,17 +22,22 @@
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
-                        "installed_by": ["modules"]
+                        "git_sha": "d086322563bdbb08c94bf15a7db58a39ccdb1520",
+                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
-                        "installed_by": ["modules"]
+                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
+                        "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
+                        "installed_by": ["modules"]
+                    },
+                    "samtools/bam2fq": {
+                        "branch": "master",
+                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905",
                         "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
@@ -54,6 +64,16 @@
                         "branch": "master",
                         "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
                         "installed_by": ["modules", "bam_stats_samtools"]
+                    },
+                    "umitools/dedup": {
+                        "branch": "master",
+                        "git_sha": "9d489648a7425fa18d8bed18935442c104d8733c",
+                        "installed_by": ["modules"]
+                    },
+                    "umitools/extract": {
+                        "branch": "master",
+                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905",
+                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     }
                 }
             },
@@ -68,6 +88,11 @@
                         "branch": "master",
                         "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f",
                         "installed_by": ["subworkflows", "bam_sort_stats_samtools"]
+                    },
+                    "fastq_fastqc_umitools_fastp": {
+                        "branch": "master",
+                        "git_sha": "3e8b0c1144ccf60b7848efbdc2be285ff20b49ee",
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
diff --git a/modules/nf-core/fastp/environment.yml b/modules/nf-core/fastp/environment.yml
index 19ccec25..70389e66 100644
--- a/modules/nf-core/fastp/environment.yml
+++ b/modules/nf-core/fastp/environment.yml
@@ -1,3 +1,4 @@
+name: fastp
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf
index ca5f100f..2a3b679e 100644
--- a/modules/nf-core/fastp/main.nf
+++ b/modules/nf-core/fastp/main.nf
@@ -2,7 +2,7 @@ process FASTP {
     tag "$meta.id"
     label 'process_medium'
 
-    conda 'modules/nf-core/fastp/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/fastp:0.23.4--h5f740d0_0' :
         'biocontainers/fastp:0.23.4--h5f740d0_0' }"
@@ -45,7 +45,7 @@ process FASTP {
             $adapter_list \\
             $fail_fastq \\
             $args \\
-            2> ${prefix}.fastp.log \\
+            2> >(tee ${prefix}.fastp.log >&2) \\
         | gzip -c > ${prefix}.fastp.fastq.gz
 
         cat <<-END_VERSIONS > versions.yml
@@ -66,7 +66,7 @@ process FASTP {
             $adapter_list \\
             $fail_fastq \\
             $args \\
-            2> ${prefix}.fastp.log
+            2> >(tee ${prefix}.fastp.log >&2)
 
         cat <<-END_VERSIONS > versions.yml
         "${task.process}":
@@ -91,7 +91,7 @@ process FASTP {
             --thread $task.cpus \\
             --detect_adapter_for_pe \\
             $args \\
-            2> ${prefix}.fastp.log
+            2> >(tee ${prefix}.fastp.log >&2)
 
         cat <<-END_VERSIONS > versions.yml
         "${task.process}":
@@ -99,4 +99,22 @@ process FASTP {
         END_VERSIONS
         """
     }
+
+    stub:
+    def prefix              = task.ext.prefix ?: "${meta.id}"
+    def is_single_output    = task.ext.args?.contains('--interleaved_in') || meta.single_end
+    def touch_reads         = is_single_output ? "${prefix}.fastp.fastq.gz" : "${prefix}_1.fastp.fastq.gz ${prefix}_2.fastp.fastq.gz"
+    def touch_merged        = (!is_single_output && save_merged) ? "touch ${prefix}.merged.fastq.gz" : ""
+    """
+    touch $touch_reads
+    touch "${prefix}.fastp.json"
+    touch "${prefix}.fastp.html"
+    touch "${prefix}.fastp.log"
+    $touch_merged
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g")
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test
index f610b735..17dce8ac 100644
--- a/modules/nf-core/fastp/tests/main.nf.test
+++ b/modules/nf-core/fastp/tests/main.nf.test
@@ -57,6 +57,67 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { file(it[1]).getName() } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_single_end-for_stub_match")
+                },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("test_fastp_single_end-stub") {
+
+        options '-stub'
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+
+                input[0] = [
+                    [ id:'test', single_end:true ],
+                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
+                ]
+
+                input[1] = adapter_fasta
+                input[2] = save_trimmed_fail
+                input[3] = save_merged
+                """
+            }
+        }
+
+        then {
+
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { file(it[1]).getName() } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_single_end-for_stub_match")
+                },
                 { assert snapshot(process.out.versions).match("versions") }
             )
         }
@@ -127,6 +188,67 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_paired_end-for_stub_match")
+                },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("test_fastp_paired_end-stub") {
+
+        options '-stub'
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
+                ]
+
+                input[1] = adapter_fasta
+                input[2] = save_trimmed_fail
+                input[3] = save_merged
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_paired_end-for_stub_match")
+                },
                 { assert snapshot(process.out.versions).match("versions") }
             )
         }
@@ -181,6 +303,66 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { file(it[1]).getName() } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_interleaved-for_stub_match")
+                },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("fastp test_fastp_interleaved-stub") {
+
+        options '-stub'
+
+        config './nextflow.config'
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+
+                input[0] = [ [ id:'test', single_end:true ], // meta map
+                            [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ]
+                        ]
+
+                input[1] = adapter_fasta
+                input[2] = save_trimmed_fail
+                input[3] = save_merged
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { file(it[1]).getName() } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_interleaved-for_stub_match")
+                },
                 { assert snapshot(process.out.versions).match("versions") }
             )
         }
@@ -399,6 +581,65 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_paired_end_merged-for_stub_match")
+                },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("test_fastp_paired_end_merged-stub") {
+
+        options '-stub'
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = true
+
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                             [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                               file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
+                           ]
+                input[1] = adapter_fasta
+                input[2] = save_trimmed_fail
+                input[3] = save_merged
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_paired_end_merged-for_stub_match")
+                },
                 { assert snapshot(process.out.versions).match("versions") }
             )
         }
diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap
index 0fa68c7d..1b7d2419 100644
--- a/modules/nf-core/fastp/tests/main.nf.test.snap
+++ b/modules/nf-core/fastp/tests/main.nf.test.snap
@@ -1,4 +1,19 @@
 {
+    "test_fastp_paired_end-for_stub_match": {
+        "content": [
+            [
+                [
+                    "test_1.fastp.fastq.gz",
+                    "test_2.fastp.fastq.gz"
+                ],
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=false}"
+            ]
+        ],
+        "timestamp": "2023-12-21T09:44:37.202512"
+    },
     "fastp test_fastp_interleaved_json": {
         "content": [
             [
@@ -13,6 +28,22 @@
         ],
         "timestamp": "2023-10-17T11:04:45.794175881"
     },
+    "test_fastp_paired_end_merged-for_stub_match": {
+        "content": [
+            [
+                [
+                    "test_1.fastp.fastq.gz",
+                    "test_2.fastp.fastq.gz"
+                ],
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "test.merged.fastq.gz",
+                "{id=test, single_end=false}"
+            ]
+        ],
+        "timestamp": "2023-12-21T09:53:45.237014"
+    },
     "test_fastp_single_end_json": {
         "content": [
             [
@@ -35,6 +66,30 @@
         ],
         "timestamp": "2023-10-17T11:04:10.582076024"
     },
+    "test_fastp_interleaved-for_stub_match": {
+        "content": [
+            [
+                "test.fastp.fastq.gz",
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=true}"
+            ]
+        ],
+        "timestamp": "2023-12-21T09:48:43.148485"
+    },
+    "test_fastp_single_end-for_stub_match": {
+        "content": [
+            [
+                "test.fastp.fastq.gz",
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=true}"
+            ]
+        ],
+        "timestamp": "2023-12-21T09:20:07.254788"
+    },
     "test_fastp_single_end_trim_fail_json": {
         "content": [
             [
diff --git a/modules/nf-core/modules/cat/cat/main.nf b/modules/nf-core/modules/cat/cat/main.nf
deleted file mode 100644
index 40e53f3e..00000000
--- a/modules/nf-core/modules/cat/cat/main.nf
+++ /dev/null
@@ -1,62 +0,0 @@
-process CAT_CAT {
-    tag "$meta.id"
-    label 'process_low'
-
-    conda (params.enable_conda ? "conda-forge::pigz=2.3.4" : null)
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/pigz:2.3.4' :
-        'quay.io/biocontainers/pigz:2.3.4' }"
-
-    input:
-    tuple val(meta), path(files_in)
-
-    output:
-    tuple val(meta), path("${prefix}"), emit: file_out
-    path "versions.yml"               , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    def file_list = files_in.collect { it.toString() }
-
-    // | input     | output     | command1 | command2 |
-    // |-----------|------------|----------|----------|
-    // | gzipped   | gzipped    | cat      |          |
-    // | ungzipped | ungzipped  | cat      |          |
-    // | gzipped   | ungzipped  | zcat     |          |
-    // | ungzipped | gzipped    | cat      | pigz     |
-
-    // Use input file ending as default
-    prefix   = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
-    out_zip  = prefix.endsWith('.gz')
-    in_zip   = file_list[0].endsWith('.gz')
-    command1 = (in_zip && !out_zip) ? 'zcat' : 'cat'
-    command2 = (!in_zip && out_zip) ? "| pigz -c -p $task.cpus $args2" : ''
-    """
-    $command1 \\
-        $args \\
-        ${file_list.join(' ')} \\
-        $command2 \\
-        > ${prefix}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
-    END_VERSIONS
-    """
-
-    stub:
-    def file_list = files_in.collect { it.toString() }
-    prefix   = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
-    """
-    touch $prefix
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/modules/cat/cat/meta.yml b/modules/nf-core/modules/cat/cat/meta.yml
deleted file mode 100644
index 5eeff5a6..00000000
--- a/modules/nf-core/modules/cat/cat/meta.yml
+++ /dev/null
@@ -1,37 +0,0 @@
-name: cat_cat
-description: A module for concatenation of gzipped or uncompressed files
-keywords:
-  - concatenate
-  - gzip
-  - cat
-tools:
-  - cat:
-      description: Just concatenation
-      homepage: None
-      documentation: https://man7.org/linux/man-pages/man1/cat.1.html
-      tool_dev_url: None
-      licence: ["GPL-3.0-or-later"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - files_in:
-      type: file
-      description: List of compressed / uncompressed files
-      pattern: "*"
-
-output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - file_out:
-      type: file
-      description: Concatenated file. Will be gzipped if file_out ends with ".gz"
-      pattern: "${file_out}"
-
-authors:
-  - "@erikrikarddaniel"
-  - "@FriederikeHanssen"
diff --git a/modules/nf-core/modules/samtools/bam2fq/main.nf b/modules/nf-core/modules/samtools/bam2fq/main.nf
deleted file mode 100644
index 9301d1d3..00000000
--- a/modules/nf-core/modules/samtools/bam2fq/main.nf
+++ /dev/null
@@ -1,56 +0,0 @@
-process SAMTOOLS_BAM2FQ {
-    tag "$meta.id"
-    label 'process_low'
-
-    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
-        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
-
-    input:
-    tuple val(meta), path(inputbam)
-    val split
-
-    output:
-    tuple val(meta), path("*.fq.gz"), emit: reads
-    path "versions.yml"             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-
-    if (split){
-        """
-        samtools \\
-            bam2fq \\
-            $args \\
-            -@ $task.cpus \\
-            -1 ${prefix}_1.fq.gz \\
-            -2 ${prefix}_2.fq.gz \\
-            -0 ${prefix}_other.fq.gz \\
-            -s ${prefix}_singleton.fq.gz \\
-            $inputbam
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-        END_VERSIONS
-        """
-    } else {
-        """
-        samtools \\
-            bam2fq \\
-            $args \\
-            -@ $task.cpus \\
-            $inputbam | gzip --no-name > ${prefix}_interleaved.fq.gz
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-        END_VERSIONS
-        """
-    }
-}
diff --git a/modules/nf-core/modules/samtools/bam2fq/meta.yml b/modules/nf-core/modules/samtools/bam2fq/meta.yml
deleted file mode 100644
index 319a60cf..00000000
--- a/modules/nf-core/modules/samtools/bam2fq/meta.yml
+++ /dev/null
@@ -1,55 +0,0 @@
-name: samtools_bam2fq
-description: |
-  The module uses bam2fq method from samtools to
-  convert a SAM, BAM or CRAM file to FASTQ format
-keywords:
-  - bam2fq
-  - samtools
-  - fastq
-tools:
-  - samtools:
-      description: Tools for dealing with SAM, BAM and CRAM files
-      homepage: None
-      documentation: http://www.htslib.org/doc/1.1/samtools.html
-      tool_dev_url: None
-      doi: ""
-      licence: ["MIT"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - inputbam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - split:
-      type: boolean
-      description: |
-        TRUE/FALSE value to indicate if reads should be separated into
-        /1, /2 and if present other, or singleton.
-        Note: choosing TRUE will generate 4 different files.
-        Choosing FALSE will produce a single file, which will be interleaved in case
-        the input contains paired reads.
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - reads:
-      type: file
-      description: |
-        FASTQ files, which will be either a group of 4 files (read_1, read_2, other and singleton)
-        or a single interleaved .fq.gz file if the user chooses not to split the reads.
-      pattern: "*.fq.gz"
-
-authors:
-  - "@lescai"
diff --git a/modules/nf-core/modules/umitools/dedup/main.nf b/modules/nf-core/modules/umitools/dedup/main.nf
deleted file mode 100644
index dfcbcf2f..00000000
--- a/modules/nf-core/modules/umitools/dedup/main.nf
+++ /dev/null
@@ -1,41 +0,0 @@
-process UMITOOLS_DEDUP {
-    tag "$meta.id"
-    label "process_medium"
-
-    conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
-        'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
-
-    input:
-    tuple val(meta), path(bam), path(bai)
-
-    output:
-    tuple val(meta), path("*.bam")             , emit: bam
-    tuple val(meta), path("*edit_distance.tsv"), emit: tsv_edit_distance
-    tuple val(meta), path("*per_umi.tsv")      , emit: tsv_per_umi
-    tuple val(meta), path("*per_position.tsv") , emit: tsv_umi_per_position
-    path  "versions.yml"                       , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def paired = meta.single_end ? "" : "--paired"
-    """
-    umi_tools \\
-        dedup \\
-        -I $bam \\
-        -S ${prefix}.bam \\
-        --output-stats $prefix \\
-        $paired \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/modules/umitools/dedup/meta.yml b/modules/nf-core/modules/umitools/dedup/meta.yml
deleted file mode 100644
index eee8952f..00000000
--- a/modules/nf-core/modules/umitools/dedup/meta.yml
+++ /dev/null
@@ -1,59 +0,0 @@
-name: umitools_dedup
-description: Deduplicate reads based on the mapping co-ordinate and the UMI attached to the read.
-keywords:
-  - umitools
-  - deduplication
-tools:
-  - umi_tools:
-    description: >
-      UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs)
-      and single cell RNA-Seq cell barcodes
-    documentation: https://umi-tools.readthedocs.io/en/latest/
-    license: ["MIT"]
-input:
-  - meta:
-    type: map
-    description: |
-      Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-    type: file
-    description: |
-      BAM file containing reads to be deduplicated via UMIs.
-    pattern: "*.{bam}"
-  - bai:
-    type: file
-    description: |
-      BAM index files corresponding to the input BAM file.
-    pattern: "*.{bai}"
-output:
-  - meta:
-    type: map
-    description: |
-      Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-    type: file
-    description: BAM file with deduplicated UMIs.
-    pattern: "*.{bam}"
-  - tsv_edit_distance:
-    type: file
-    description: Reports the (binned) average edit distance between the UMIs at each position.
-    pattern: "*edit_distance.tsv"
-  - tsv_per_umi:
-    type: file
-    description: UMI-level summary statistics.
-    pattern: "*per_umi.tsv"
-  - tsv_umi_per_position:
-    type: file
-    description: Tabulates the counts for unique combinations of UMI and position.
-    pattern: "*per_position.tsv"
-  - versions:
-    type: file
-    description: File containing software versions
-    pattern: "versions.yml"
-
-authors:
-  - "@drpatelh"
-  - "@grst"
-  - "@klkeys"
diff --git a/modules/nf-core/modules/umitools/extract/main.nf b/modules/nf-core/modules/umitools/extract/main.nf
deleted file mode 100644
index 22a405b9..00000000
--- a/modules/nf-core/modules/umitools/extract/main.nf
+++ /dev/null
@@ -1,55 +0,0 @@
-process UMITOOLS_EXTRACT {
-    tag "$meta.id"
-    label "process_low"
-
-    conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
-        'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
-
-    input:
-    tuple val(meta), path(reads)
-
-    output:
-    tuple val(meta), path("*.fastq.gz"), emit: reads
-    tuple val(meta), path("*.log")     , emit: log
-    path  "versions.yml"               , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    if (meta.single_end) {
-        """
-        umi_tools \\
-            extract \\
-            -I $reads \\
-            -S ${prefix}.umi_extract.fastq.gz \\
-            $args \\
-            > ${prefix}.umi_extract.log
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
-        END_VERSIONS
-        """
-    }  else {
-        """
-        umi_tools \\
-            extract \\
-            -I ${reads[0]} \\
-            --read2-in=${reads[1]} \\
-            -S ${prefix}.umi_extract_1.fastq.gz \\
-            --read2-out=${prefix}.umi_extract_2.fastq.gz \\
-            $args \\
-            > ${prefix}.umi_extract.log
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
-        END_VERSIONS
-        """
-    }
-}
diff --git a/modules/nf-core/modules/umitools/extract/meta.yml b/modules/nf-core/modules/umitools/extract/meta.yml
deleted file mode 100644
index 7fc23f72..00000000
--- a/modules/nf-core/modules/umitools/extract/meta.yml
+++ /dev/null
@@ -1,47 +0,0 @@
-name: umitools_extract
-description: Extracts UMI barcode from a read and add it to the read name, leaving any sample barcode in place
-keywords:
-  - umitools
-  - extract
-tools:
-  - umi_tools:
-    description: >
-      UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs)
-      and single cell RNA-Seq cell barcodes
-    documentation: https://umi-tools.readthedocs.io/en/latest/
-    license: ["MIT"]
-input:
-  - meta:
-    type: map
-    description: |
-      Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-    type: list
-    description: |
-      List of input FASTQ files whose UMIs will be extracted.
-output:
-  - meta:
-    type: map
-    description: |
-      Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-    type: file
-    description: >
-      Extracted FASTQ files. |
-      For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. |
-        For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
-    pattern: "*.{fastq.gz}"
-  - log:
-    type: file
-    description: Logfile for umi_tools
-    pattern: "*.{log}"
-  - versions:
-    type: file
-    description: File containing software versions
-    pattern: "versions.yml"
-
-authors:
-  - "@drpatelh"
-  - "@grst"
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index 9d0e6b20..7625b752 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -1,6 +1,7 @@
+name: multiqc
 channels:
   - conda-forge
   - bioconda
   - defaults
 dependencies:
-  - bioconda::multiqc=1.17
+  - bioconda::multiqc=1.19
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 485b3ba8..1b9f7c43 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -1,10 +1,10 @@
 process MULTIQC {
     label 'process_single'
 
-    conda 'modules/nf-core/multiqc/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.17--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.19--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
@@ -25,12 +25,14 @@ process MULTIQC {
     def args = task.ext.args ?: ''
     def config = multiqc_config ? "--config $multiqc_config" : ''
     def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
+    def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : ''
     """
     multiqc \\
         --force \\
         $args \\
         $config \\
         $extra_config \\
+        $logo \\
         .
 
     cat <<-END_VERSIONS > versions.yml
@@ -41,7 +43,7 @@ process MULTIQC {
 
     stub:
     """
-    touch multiqc_data
+    mkdir multiqc_data
     touch multiqc_plots
     touch multiqc_report.html
 
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index a61223ed..45a9bc35 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,5 +1,4 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
-name: MultiQC
+name: multiqc
 description: Aggregate results from bioinformatics analyses across many samples into a single report
 keywords:
   - QC

From fed23d1644862c285001275cc421071c4a0911ec Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 11 Jan 2024 13:02:09 +0000
Subject: [PATCH 055/227] Add missing modules / subworkflows

---
 modules/nf-core/cat/cat/environment.yml       |   7 +
 modules/nf-core/cat/cat/main.nf               |  70 +++++++
 modules/nf-core/cat/cat/meta.yml              |  36 ++++
 modules/nf-core/cat/cat/tests/main.nf.test    | 179 ++++++++++++++++++
 .../nf-core/cat/cat/tests/main.nf.test.snap   | 121 ++++++++++++
 .../cat/tests/nextflow_unzipped_zipped.config |   6 +
 .../cat/tests/nextflow_zipped_unzipped.config |   8 +
 modules/nf-core/cat/cat/tests/tags.yml        |   2 +
 modules/nf-core/multiqc/tests/main.nf.test    |  83 ++++++++
 .../nf-core/multiqc/tests/main.nf.test.snap   |  21 ++
 modules/nf-core/multiqc/tests/tags.yml        |   2 +
 modules/nf-core/samtools/bam2fq/main.nf       |  56 ++++++
 modules/nf-core/samtools/bam2fq/meta.yml      |  55 ++++++
 modules/nf-core/umitools/dedup/main.nf        |  41 ++++
 modules/nf-core/umitools/dedup/meta.yml       |  59 ++++++
 modules/nf-core/umitools/extract/main.nf      |  55 ++++++
 modules/nf-core/umitools/extract/meta.yml     |  47 +++++
 .../fastq_fastqc_umitools_fastp/main.nf       | 140 ++++++++++++++
 .../fastq_fastqc_umitools_fastp/meta.yml      | 128 +++++++++++++
 .../tests/main.nf.test                        |  60 ++++++
 .../tests/main.nf.test.snap                   |  81 ++++++++
 .../tests/tags.yml                            |   2 +
 22 files changed, 1259 insertions(+)
 create mode 100644 modules/nf-core/cat/cat/environment.yml
 create mode 100644 modules/nf-core/cat/cat/main.nf
 create mode 100644 modules/nf-core/cat/cat/meta.yml
 create mode 100644 modules/nf-core/cat/cat/tests/main.nf.test
 create mode 100644 modules/nf-core/cat/cat/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config
 create mode 100644 modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config
 create mode 100644 modules/nf-core/cat/cat/tests/tags.yml
 create mode 100644 modules/nf-core/multiqc/tests/main.nf.test
 create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/multiqc/tests/tags.yml
 create mode 100644 modules/nf-core/samtools/bam2fq/main.nf
 create mode 100644 modules/nf-core/samtools/bam2fq/meta.yml
 create mode 100644 modules/nf-core/umitools/dedup/main.nf
 create mode 100644 modules/nf-core/umitools/dedup/meta.yml
 create mode 100644 modules/nf-core/umitools/extract/main.nf
 create mode 100644 modules/nf-core/umitools/extract/meta.yml
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/tags.yml

diff --git a/modules/nf-core/cat/cat/environment.yml b/modules/nf-core/cat/cat/environment.yml
new file mode 100644
index 00000000..17a04ef2
--- /dev/null
+++ b/modules/nf-core/cat/cat/environment.yml
@@ -0,0 +1,7 @@
+name: cat_cat
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - conda-forge::pigz=2.3.4
diff --git a/modules/nf-core/cat/cat/main.nf b/modules/nf-core/cat/cat/main.nf
new file mode 100644
index 00000000..970ab760
--- /dev/null
+++ b/modules/nf-core/cat/cat/main.nf
@@ -0,0 +1,70 @@
+process CAT_CAT {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/pigz:2.3.4' :
+        'biocontainers/pigz:2.3.4' }"
+
+    input:
+    tuple val(meta), path(files_in)
+
+    output:
+    tuple val(meta), path("${prefix}"), emit: file_out
+    path "versions.yml"               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def file_list = files_in.collect { it.toString() }
+
+    // | input     | output     | command1 | command2 |
+    // |-----------|------------|----------|----------|
+    // | gzipped   | gzipped    | cat      |          |
+    // | ungzipped | ungzipped  | cat      |          |
+    // | gzipped   | ungzipped  | zcat     |          |
+    // | ungzipped | gzipped    | cat      | pigz     |
+
+    // Use input file ending as default
+    prefix   = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
+    out_zip  = prefix.endsWith('.gz')
+    in_zip   = file_list[0].endsWith('.gz')
+    command1 = (in_zip && !out_zip) ? 'zcat' : 'cat'
+    command2 = (!in_zip && out_zip) ? "| pigz -c -p $task.cpus $args2" : ''
+    if(file_list.contains(prefix.trim())) {
+        error "The name of the input file can't be the same as for the output prefix in the " +
+        "module CAT_CAT (currently `$prefix`). Please choose a different one."
+    }
+    """
+    $command1 \\
+        $args \\
+        ${file_list.join(' ')} \\
+        $command2 \\
+        > ${prefix}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
+    END_VERSIONS
+    """
+
+    stub:
+    def file_list   = files_in.collect { it.toString() }
+    prefix          = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
+    if(file_list.contains(prefix.trim())) {
+        error "The name of the input file can't be the same as for the output prefix in the " +
+        "module CAT_CAT (currently `$prefix`). Please choose a different one."
+    }
+    """
+    touch $prefix
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/cat/cat/meta.yml b/modules/nf-core/cat/cat/meta.yml
new file mode 100644
index 00000000..00a8db0b
--- /dev/null
+++ b/modules/nf-core/cat/cat/meta.yml
@@ -0,0 +1,36 @@
+name: cat_cat
+description: A module for concatenation of gzipped or uncompressed files
+keywords:
+  - concatenate
+  - gzip
+  - cat
+tools:
+  - cat:
+      description: Just concatenation
+      documentation: https://man7.org/linux/man-pages/man1/cat.1.html
+      licence: ["GPL-3.0-or-later"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - files_in:
+      type: file
+      description: List of compressed / uncompressed files
+      pattern: "*"
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - file_out:
+      type: file
+      description: Concatenated file. Will be gzipped if file_out ends with ".gz"
+      pattern: "${file_out}"
+authors:
+  - "@erikrikarddaniel"
+  - "@FriederikeHanssen"
+maintainers:
+  - "@erikrikarddaniel"
+  - "@FriederikeHanssen"
diff --git a/modules/nf-core/cat/cat/tests/main.nf.test b/modules/nf-core/cat/cat/tests/main.nf.test
new file mode 100644
index 00000000..ed5a4f12
--- /dev/null
+++ b/modules/nf-core/cat/cat/tests/main.nf.test
@@ -0,0 +1,179 @@
+nextflow_process {
+
+    name "Test Process CAT_CAT"
+    script "../main.nf"
+    process "CAT_CAT"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "cat"
+    tag "cat/cat"
+
+    test("test_cat_name_conflict") {
+        when {
+            params {
+                outdir   = "${outputDir}"
+            }
+            process {
+                """
+                input[0] =
+                    [
+                        [ id:'genome', single_end:true ],
+                        [
+                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true)
+                        ]
+                    ]
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert !process.success },
+                { assert process.stdout.toString().contains("The name of the input file can't be the same as for the output prefix") }
+            )
+        }
+    }
+
+    test("test_cat_unzipped_unzipped") {
+        when {
+            params {
+                outdir   = "${outputDir}"
+            }
+            process {
+                """
+                input[0] =
+                    [
+                        [ id:'test', single_end:true ],
+                        [
+                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true)
+                        ]
+                    ]
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+
+    test("test_cat_zipped_zipped") {
+        when {
+            params {
+                outdir   = "${outputDir}"
+            }
+            process {
+                """
+                input[0] =
+                    [
+                        [ id:'test', single_end:true ],
+                        [
+                            file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true)
+                        ]
+                    ]
+                """
+            }
+        }
+        then {
+            def lines = path(process.out.file_out.get(0).get(1)).linesGzip
+            assertAll(
+                { assert process.success },
+                { assert snapshot(lines[0..5]).match("test_cat_zipped_zipped_lines") },
+                { assert snapshot(lines.size()).match("test_cat_zipped_zipped_size")}
+            )
+        }
+    }
+
+    test("test_cat_zipped_unzipped") {
+        config './nextflow_zipped_unzipped.config'
+
+        when {
+            params {
+                outdir   = "${outputDir}"
+            }
+            process {
+                """
+                input[0] =
+                    [
+                        [ id:'test', single_end:true ],
+                        [
+                            file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true)
+                        ]
+                    ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("test_cat_unzipped_zipped") {
+        config './nextflow_unzipped_zipped.config'
+        when {
+            params {
+                outdir   = "${outputDir}"
+            }
+            process {
+                """
+                input[0] =
+                    [
+                        [ id:'test', single_end:true ],
+                        [
+                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true)
+                        ]
+                    ]
+                """
+            }
+        }
+        then {
+            def lines = path(process.out.file_out.get(0).get(1)).linesGzip
+            assertAll(
+                { assert process.success },
+                { assert snapshot(lines[0..5]).match("test_cat_unzipped_zipped_lines") },
+                { assert snapshot(lines.size()).match("test_cat_unzipped_zipped_size")}
+            )
+        }
+    }
+
+    test("test_cat_one_file_unzipped_zipped") {
+        config './nextflow_unzipped_zipped.config'
+        when {
+            params {
+                outdir   = "${outputDir}"
+            }
+            process {
+                """
+                input[0] =
+                    [
+                        [ id:'test', single_end:true ],
+                        [
+                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                        ]
+                    ]
+                """
+            }
+        }
+        then {
+            def lines = path(process.out.file_out.get(0).get(1)).linesGzip
+            assertAll(
+                { assert process.success },
+                { assert snapshot(lines[0..5]).match("test_cat_one_file_unzipped_zipped_lines") },
+                { assert snapshot(lines.size()).match("test_cat_one_file_unzipped_zipped_size")}
+            )
+        }
+    }
+}
+
diff --git a/modules/nf-core/cat/cat/tests/main.nf.test.snap b/modules/nf-core/cat/cat/tests/main.nf.test.snap
new file mode 100644
index 00000000..423571ba
--- /dev/null
+++ b/modules/nf-core/cat/cat/tests/main.nf.test.snap
@@ -0,0 +1,121 @@
+{
+    "test_cat_unzipped_zipped_size": {
+        "content": [
+            375
+        ],
+        "timestamp": "2023-10-16T14:33:08.049445686"
+    },
+    "test_cat_unzipped_unzipped": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
+                ],
+                "file_out": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
+                ]
+            }
+        ],
+        "timestamp": "2023-10-16T14:32:18.500464399"
+    },
+    "test_cat_zipped_unzipped": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
+                ],
+                "file_out": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
+                ]
+            }
+        ],
+        "timestamp": "2023-10-16T14:32:49.642741302"
+    },
+    "test_cat_zipped_zipped_lines": {
+        "content": [
+            [
+                "MT192765.1\tGenbank\ttranscript\t259\t29667\t.\t+\t.\tID=unknown_transcript_1;geneID=orf1ab;gene_name=orf1ab",
+                "MT192765.1\tGenbank\tgene\t259\t21548\t.\t+\t.\tParent=unknown_transcript_1",
+                "MT192765.1\tGenbank\tCDS\t259\t13461\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1",
+                "MT192765.1\tGenbank\tCDS\t13461\t21548\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1",
+                "MT192765.1\tGenbank\tCDS\t21556\t25377\t.\t+\t0\tParent=unknown_transcript_1;gbkey=CDS;gene=S;note=\"structural protein\";product=\"surface glycoprotein\";protein_id=QIK50427.1",
+                "MT192765.1\tGenbank\tgene\t21556\t25377\t.\t+\t.\tParent=unknown_transcript_1"
+            ]
+        ],
+        "timestamp": "2023-10-16T14:32:33.629048645"
+    },
+    "test_cat_unzipped_zipped_lines": {
+        "content": [
+            [
+                ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome",
+                "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT",
+                "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG",
+                "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG",
+                "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT",
+                "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG"
+            ]
+        ],
+        "timestamp": "2023-10-16T14:33:08.038830506"
+    },
+    "test_cat_one_file_unzipped_zipped_lines": {
+        "content": [
+            [
+                ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome",
+                "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT",
+                "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG",
+                "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG",
+                "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT",
+                "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG"
+            ]
+        ],
+        "timestamp": "2023-10-16T14:33:21.39642399"
+    },
+    "test_cat_zipped_zipped_size": {
+        "content": [
+            78
+        ],
+        "timestamp": "2023-10-16T14:32:33.641869244"
+    },
+    "test_cat_one_file_unzipped_zipped_size": {
+        "content": [
+            374
+        ],
+        "timestamp": "2023-10-16T14:33:21.4094373"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config b/modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config
new file mode 100644
index 00000000..ec26b0fd
--- /dev/null
+++ b/modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config
@@ -0,0 +1,6 @@
+
+process {
+    withName: CAT_CAT {
+        ext.prefix = 'cat.txt.gz'
+    }
+}
diff --git a/modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config b/modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config
new file mode 100644
index 00000000..fbc79783
--- /dev/null
+++ b/modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config
@@ -0,0 +1,8 @@
+
+process {
+
+    withName: CAT_CAT {
+        ext.prefix = 'cat.txt'
+    }
+
+}
diff --git a/modules/nf-core/cat/cat/tests/tags.yml b/modules/nf-core/cat/cat/tests/tags.yml
new file mode 100644
index 00000000..37b578f5
--- /dev/null
+++ b/modules/nf-core/cat/cat/tests/tags.yml
@@ -0,0 +1,2 @@
+cat/cat:
+  - modules/nf-core/cat/cat/**
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
new file mode 100644
index 00000000..d0438eda
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -0,0 +1,83 @@
+nextflow_process {
+
+    name "Test Process MULTIQC"
+    script "../main.nf"
+    process "MULTIQC"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "multiqc"
+
+    test("sarscov2 single-end [fastqc]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+
+    }
+
+    test("sarscov2 single-end [fastqc] [config]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastqc] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.report.collect { file(it).getName() } +
+                                process.out.data.collect { file(it).getName() } +
+                                process.out.plots.collect { file(it).getName() } +
+                                process.out.versions ).match() }
+            )
+        }
+
+    }
+}
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
new file mode 100644
index 00000000..d37e7304
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -0,0 +1,21 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d"
+            ]
+        ],
+        "timestamp": "2024-01-09T23:02:49.911994"
+    },
+    "sarscov2 single-end [fastqc] - stub": {
+        "content": [
+            [
+                "multiqc_report.html",
+                "multiqc_data",
+                "multiqc_plots",
+                "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d"
+            ]
+        ],
+        "timestamp": "2024-01-09T23:03:14.524346"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml
new file mode 100644
index 00000000..bea6c0d3
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/tags.yml
@@ -0,0 +1,2 @@
+multiqc:
+  - modules/nf-core/multiqc/**
diff --git a/modules/nf-core/samtools/bam2fq/main.nf b/modules/nf-core/samtools/bam2fq/main.nf
new file mode 100644
index 00000000..9301d1d3
--- /dev/null
+++ b/modules/nf-core/samtools/bam2fq/main.nf
@@ -0,0 +1,56 @@
+process SAMTOOLS_BAM2FQ {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
+        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
+
+    input:
+    tuple val(meta), path(inputbam)
+    val split
+
+    output:
+    tuple val(meta), path("*.fq.gz"), emit: reads
+    path "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    if (split){
+        """
+        samtools \\
+            bam2fq \\
+            $args \\
+            -@ $task.cpus \\
+            -1 ${prefix}_1.fq.gz \\
+            -2 ${prefix}_2.fq.gz \\
+            -0 ${prefix}_other.fq.gz \\
+            -s ${prefix}_singleton.fq.gz \\
+            $inputbam
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+        END_VERSIONS
+        """
+    } else {
+        """
+        samtools \\
+            bam2fq \\
+            $args \\
+            -@ $task.cpus \\
+            $inputbam | gzip --no-name > ${prefix}_interleaved.fq.gz
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+        END_VERSIONS
+        """
+    }
+}
diff --git a/modules/nf-core/samtools/bam2fq/meta.yml b/modules/nf-core/samtools/bam2fq/meta.yml
new file mode 100644
index 00000000..319a60cf
--- /dev/null
+++ b/modules/nf-core/samtools/bam2fq/meta.yml
@@ -0,0 +1,55 @@
+name: samtools_bam2fq
+description: |
+  The module uses bam2fq method from samtools to
+  convert a SAM, BAM or CRAM file to FASTQ format
+keywords:
+  - bam2fq
+  - samtools
+  - fastq
+tools:
+  - samtools:
+      description: Tools for dealing with SAM, BAM and CRAM files
+      homepage: None
+      documentation: http://www.htslib.org/doc/1.1/samtools.html
+      tool_dev_url: None
+      doi: ""
+      licence: ["MIT"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - inputbam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - split:
+      type: boolean
+      description: |
+        TRUE/FALSE value to indicate if reads should be separated into
+        /1, /2 and if present other, or singleton.
+        Note: choosing TRUE will generate 4 different files.
+        Choosing FALSE will produce a single file, which will be interleaved in case
+        the input contains paired reads.
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - reads:
+      type: file
+      description: |
+        FASTQ files, which will be either a group of 4 files (read_1, read_2, other and singleton)
+        or a single interleaved .fq.gz file if the user chooses not to split the reads.
+      pattern: "*.fq.gz"
+
+authors:
+  - "@lescai"
diff --git a/modules/nf-core/umitools/dedup/main.nf b/modules/nf-core/umitools/dedup/main.nf
new file mode 100644
index 00000000..dfcbcf2f
--- /dev/null
+++ b/modules/nf-core/umitools/dedup/main.nf
@@ -0,0 +1,41 @@
+process UMITOOLS_DEDUP {
+    tag "$meta.id"
+    label "process_medium"
+
+    conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
+        'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+
+    output:
+    tuple val(meta), path("*.bam")             , emit: bam
+    tuple val(meta), path("*edit_distance.tsv"), emit: tsv_edit_distance
+    tuple val(meta), path("*per_umi.tsv")      , emit: tsv_per_umi
+    tuple val(meta), path("*per_position.tsv") , emit: tsv_umi_per_position
+    path  "versions.yml"                       , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def paired = meta.single_end ? "" : "--paired"
+    """
+    umi_tools \\
+        dedup \\
+        -I $bam \\
+        -S ${prefix}.bam \\
+        --output-stats $prefix \\
+        $paired \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/umitools/dedup/meta.yml b/modules/nf-core/umitools/dedup/meta.yml
new file mode 100644
index 00000000..eee8952f
--- /dev/null
+++ b/modules/nf-core/umitools/dedup/meta.yml
@@ -0,0 +1,59 @@
+name: umitools_dedup
+description: Deduplicate reads based on the mapping co-ordinate and the UMI attached to the read.
+keywords:
+  - umitools
+  - deduplication
+tools:
+  - umi_tools:
+    description: >
+      UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs)
+      and single cell RNA-Seq cell barcodes
+    documentation: https://umi-tools.readthedocs.io/en/latest/
+    license: ["MIT"]
+input:
+  - meta:
+    type: map
+    description: |
+      Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+    type: file
+    description: |
+      BAM file containing reads to be deduplicated via UMIs.
+    pattern: "*.{bam}"
+  - bai:
+    type: file
+    description: |
+      BAM index files corresponding to the input BAM file.
+    pattern: "*.{bai}"
+output:
+  - meta:
+    type: map
+    description: |
+      Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+    type: file
+    description: BAM file with deduplicated UMIs.
+    pattern: "*.{bam}"
+  - tsv_edit_distance:
+    type: file
+    description: Reports the (binned) average edit distance between the UMIs at each position.
+    pattern: "*edit_distance.tsv"
+  - tsv_per_umi:
+    type: file
+    description: UMI-level summary statistics.
+    pattern: "*per_umi.tsv"
+  - tsv_umi_per_position:
+    type: file
+    description: Tabulates the counts for unique combinations of UMI and position.
+    pattern: "*per_position.tsv"
+  - versions:
+    type: file
+    description: File containing software versions
+    pattern: "versions.yml"
+
+authors:
+  - "@drpatelh"
+  - "@grst"
+  - "@klkeys"
diff --git a/modules/nf-core/umitools/extract/main.nf b/modules/nf-core/umitools/extract/main.nf
new file mode 100644
index 00000000..22a405b9
--- /dev/null
+++ b/modules/nf-core/umitools/extract/main.nf
@@ -0,0 +1,55 @@
+process UMITOOLS_EXTRACT {
+    tag "$meta.id"
+    label "process_low"
+
+    conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
+        'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+
+    output:
+    tuple val(meta), path("*.fastq.gz"), emit: reads
+    tuple val(meta), path("*.log")     , emit: log
+    path  "versions.yml"               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    if (meta.single_end) {
+        """
+        umi_tools \\
+            extract \\
+            -I $reads \\
+            -S ${prefix}.umi_extract.fastq.gz \\
+            $args \\
+            > ${prefix}.umi_extract.log
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
+        END_VERSIONS
+        """
+    }  else {
+        """
+        umi_tools \\
+            extract \\
+            -I ${reads[0]} \\
+            --read2-in=${reads[1]} \\
+            -S ${prefix}.umi_extract_1.fastq.gz \\
+            --read2-out=${prefix}.umi_extract_2.fastq.gz \\
+            $args \\
+            > ${prefix}.umi_extract.log
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
+        END_VERSIONS
+        """
+    }
+}
diff --git a/modules/nf-core/umitools/extract/meta.yml b/modules/nf-core/umitools/extract/meta.yml
new file mode 100644
index 00000000..7fc23f72
--- /dev/null
+++ b/modules/nf-core/umitools/extract/meta.yml
@@ -0,0 +1,47 @@
+name: umitools_extract
+description: Extracts UMI barcode from a read and add it to the read name, leaving any sample barcode in place
+keywords:
+  - umitools
+  - extract
+tools:
+  - umi_tools:
+    description: >
+      UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs)
+      and single cell RNA-Seq cell barcodes
+    documentation: https://umi-tools.readthedocs.io/en/latest/
+    license: ["MIT"]
+input:
+  - meta:
+    type: map
+    description: |
+      Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+    type: list
+    description: |
+      List of input FASTQ files whose UMIs will be extracted.
+output:
+  - meta:
+    type: map
+    description: |
+      Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+    type: file
+    description: >
+      Extracted FASTQ files. |
+      For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. |
+        For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
+    pattern: "*.{fastq.gz}"
+  - log:
+    type: file
+    description: Logfile for umi_tools
+    pattern: "*.{log}"
+  - versions:
+    type: file
+    description: File containing software versions
+    pattern: "versions.yml"
+
+authors:
+  - "@drpatelh"
+  - "@grst"
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
new file mode 100644
index 00000000..3dbb27ea
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
@@ -0,0 +1,140 @@
+//
+// Read QC, UMI extraction and trimming
+//
+
+include { FASTQC as FASTQC_RAW  } from '../../../modules/nf-core/fastqc/main'
+include { FASTQC as FASTQC_TRIM } from '../../../modules/nf-core/fastqc/main'
+include { UMITOOLS_EXTRACT      } from '../../../modules/nf-core/umitools/extract/main'
+include { FASTP                 } from '../../../modules/nf-core/fastp/main'
+
+//
+// Function that parses fastp json output file to get total number of reads after trimming
+//
+import groovy.json.JsonSlurper
+
+def getFastpReadsAfterFiltering(json_file) {
+    def Map json = (Map) new JsonSlurper().parseText(json_file.text).get('summary')
+    return json['after_filtering']['total_reads'].toLong()
+}
+
+workflow FASTQ_FASTQC_UMITOOLS_FASTP {
+    take:
+    reads             // channel: [ val(meta), [ reads ] ]
+    skip_fastqc       // boolean: true/false
+    with_umi          // boolean: true/false
+    skip_umi_extract  // boolean: true/false
+    umi_discard_read  // integer: 0, 1 or 2
+    skip_trimming     // boolean: true/false
+    adapter_fasta     //    file: adapter.fasta
+    save_trimmed_fail // boolean: true/false
+    save_merged       // boolean: true/false
+    min_trimmed_reads // integer: > 0
+
+    main:
+    ch_versions = Channel.empty()
+    fastqc_raw_html = Channel.empty()
+    fastqc_raw_zip  = Channel.empty()
+    if (!skip_fastqc) {
+        FASTQC_RAW (
+            reads
+        )
+        fastqc_raw_html = FASTQC_RAW.out.html
+        fastqc_raw_zip  = FASTQC_RAW.out.zip
+        ch_versions     = ch_versions.mix(FASTQC_RAW.out.versions.first())
+    }
+
+    umi_reads = reads
+    umi_log   = Channel.empty()
+    if (with_umi && !skip_umi_extract) {
+        UMITOOLS_EXTRACT (
+            reads
+        )
+        umi_reads   = UMITOOLS_EXTRACT.out.reads
+        umi_log     = UMITOOLS_EXTRACT.out.log
+        ch_versions = ch_versions.mix(UMITOOLS_EXTRACT.out.versions.first())
+
+        // Discard R1 / R2 if required
+        if (umi_discard_read in [1,2]) {
+            UMITOOLS_EXTRACT
+                .out
+                .reads
+                .map {
+                    meta, reads ->
+                        meta.single_end ? [ meta, reads ] : [ meta + [single_end: true], reads[umi_discard_read % 2] ]
+                }
+                .set { umi_reads }
+        }
+    }
+
+    trim_reads        = umi_reads
+    trim_json         = Channel.empty()
+    trim_html         = Channel.empty()
+    trim_log          = Channel.empty()
+    trim_reads_fail   = Channel.empty()
+    trim_reads_merged = Channel.empty()
+    fastqc_trim_html  = Channel.empty()
+    fastqc_trim_zip   = Channel.empty()
+    trim_read_count   = Channel.empty()
+    if (!skip_trimming) {
+        FASTP (
+            umi_reads,
+            adapter_fasta,
+            save_trimmed_fail,
+            save_merged
+        )
+        trim_json         = FASTP.out.json
+        trim_html         = FASTP.out.html
+        trim_log          = FASTP.out.log
+        trim_reads_fail   = FASTP.out.reads_fail
+        trim_reads_merged = FASTP.out.reads_merged
+        ch_versions       = ch_versions.mix(FASTP.out.versions.first())
+
+        //
+        // Filter FastQ files based on minimum trimmed read count after adapter trimming
+        //
+        FASTP
+            .out
+            .reads
+            .join(trim_json)
+            .map { meta, reads, json -> [ meta, reads, getFastpReadsAfterFiltering(json) ] }
+            .set { ch_num_trimmed_reads }
+
+        ch_num_trimmed_reads
+            .filter { meta, reads, num_reads -> num_reads >= min_trimmed_reads.toLong() }
+            .map { meta, reads, num_reads -> [ meta, reads ] }
+            .set { trim_reads }
+
+        ch_num_trimmed_reads
+            .map { meta, reads, num_reads -> [ meta, num_reads ] }
+            .set { trim_read_count }
+
+        if (!skip_fastqc) {
+            FASTQC_TRIM (
+                trim_reads
+            )
+            fastqc_trim_html = FASTQC_TRIM.out.html
+            fastqc_trim_zip  = FASTQC_TRIM.out.zip
+            ch_versions      = ch_versions.mix(FASTQC_TRIM.out.versions.first())
+        }
+    }
+
+    emit:
+    reads = trim_reads // channel: [ val(meta), [ reads ] ]
+
+    fastqc_raw_html    // channel: [ val(meta), [ html ] ]
+    fastqc_raw_zip     // channel: [ val(meta), [ zip ] ]
+
+    umi_log            // channel: [ val(meta), [ log ] ]
+
+    trim_json          // channel: [ val(meta), [ json ] ]
+    trim_html          // channel: [ val(meta), [ html ] ]
+    trim_log           // channel: [ val(meta), [ log ] ]
+    trim_reads_fail    // channel: [ val(meta), [ fastq.gz ] ]
+    trim_reads_merged  // channel: [ val(meta), [ fastq.gz ] ]
+    trim_read_count    // channel: [ val(meta), val(count) ]
+
+    fastqc_trim_html   // channel: [ val(meta), [ html ] ]
+    fastqc_trim_zip    // channel: [ val(meta), [ zip ] ]
+
+    versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ]
+}
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml
new file mode 100644
index 00000000..220e8db1
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml
@@ -0,0 +1,128 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+# yaml-language-server: $schema=yaml-schema.json
+name: "fastq_fastqc_umitools_fastp"
+description: Read QC, UMI extraction and trimming
+keywords:
+  - fastq
+  - fastqc
+  - qc
+  - UMI
+  - trimming
+  - fastp
+components:
+  - fastqc
+  - umitools/extract
+  - fastp
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
+  - skip_fastqc:
+      type: boolean
+      description: |
+        Skip fastqc process
+  - with_umi:
+      type: boolean
+      description: |
+        With or without umi detection
+  - skip_umi_extract:
+      type: boolean
+      description: |
+        With or without umi extrection
+  - umi_discard_read:
+      type: integer
+      description: |
+        Discard R1 / R2 if required
+  - skip_trimming:
+      type: boolean
+      description: |
+        Allows to skip trimgalore execution
+  - adapter_fasta:
+      type: file
+      description: |
+        Fasta file of adapter sequences
+  - save_trimmed_fail:
+      type: boolean
+      description: |
+        Save trimmed fastqs of failed samples
+  - save_merged:
+      type: boolean
+      description: |
+        Save merged fastqs
+  - min_trimmed_reads:
+      type: integer
+      description: |
+        Inputs with fewer than this reads will be filtered out of the "reads" output channel
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - reads:
+      type: file
+      description: >
+        Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. |
+
+
+
+          For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
+      pattern: "*.{fastq.gz}"
+  - fastqc_html:
+      type: file
+      description: FastQC report
+      pattern: "*_{fastqc.html}"
+  - fastqc_zip:
+      type: file
+      description: FastQC report archive
+      pattern: "*_{fastqc.zip}"
+  - log:
+      type: file
+      description: Logfile for umi_tools
+      pattern: "*.{log}"
+  - trim_json:
+      type: file
+      description: FastP Trimming report
+      pattern: "*.{fastp.json}"
+  - trim_html:
+      type: file
+      description: FastP Trimming report
+      pattern: "*.{fastp.html}"
+  - log:
+      type: file
+      description: Logfile FastP
+      pattern: "*.{fastp.log}"
+  - trim_reads_fail:
+      type: file
+      description: Trimmed fastq files failing QC
+      pattern: "*.{fastq.gz}"
+  - trim_reads_merged:
+      type: file
+      description: Trimmed and merged fastq files
+      pattern: "*.{fastq.gz}"
+  - trim_read_count:
+      type: integer
+      description: Number of reads after trimming
+  - fastqc_trim_html:
+      type: file
+      description: FastQC report
+      pattern: "*_{fastqc.html}"
+  - fastqc_trim_zip:
+      type: file
+      description: FastQC report archive
+      pattern: "*_{fastqc.zip}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@robsyme"
+maintainers:
+  - "@robsyme"
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
new file mode 100644
index 00000000..cdd73984
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
@@ -0,0 +1,60 @@
+nextflow_workflow {
+
+    name "Test Workflow FASTQ_FASTQC_UMITOOLS_FASTP"
+    script "../main.nf"
+    workflow "FASTQ_FASTQC_UMITOOLS_FASTP"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "subworkflows/fastq_fastqc_umitools_fastp"
+    tag "fastq_fastqc_umitools_fastp"
+    tag "fastqc"
+    tag "umitools/extract"
+    tag "fastp"
+
+
+    test("sarscov2 paired-end [fastq]") {
+
+        when {
+            workflow {
+                """
+                input[0] = [
+                            [ id:'test', single_end:false ], // meta map
+                            [
+                             file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                             file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                            ]
+                ]
+                input[1] = false // skip_fastqc
+                input[2] = false // with_umi
+                input[3] = false // skip_umi_extract
+                input[4] = 1     // umi_discard_read
+                input[5] = false // skip_trimming
+                input[6] = []    // adapter_fasta
+                input[7] = false // save_trimmed_fail
+                input[8] = false // save_merged
+                input[9] = 1     // min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out.reads).match("reads") },
+                { assert snapshot(workflow.out.umi_log).match("umi_log") },
+                { assert snapshot(workflow.out.trim_json).match("trim_json") },
+                { assert snapshot(workflow.out.trim_reads_fail).match("trim_reads_fail") },
+                { assert snapshot(workflow.out.trim_reads_merged).match("trim_reads_merged") },
+                { assert snapshot(workflow.out.trim_read_count).match("trim_read_count") },
+                { assert snapshot(workflow.out.versions).match("versions") },
+
+                { assert workflow.out.fastqc_raw_html },
+                { assert workflow.out.fastqc_raw_zip },
+                { assert workflow.out.trim_html },
+                { assert workflow.out.trim_log },
+                { assert workflow.out.fastqc_trim_html },
+                { assert workflow.out.fastqc_trim_zip }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
new file mode 100644
index 00000000..38a65aeb
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
@@ -0,0 +1,81 @@
+{
+    "trim_reads_merged": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "timestamp": "2023-11-26T02:28:26.26920982"
+    },
+    "trim_reads_fail": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "timestamp": "2023-11-26T02:28:26.25861515"
+    },
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,85bd0117e5778fff18e3920972a296ad",
+                "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0",
+                "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
+            ]
+        ],
+        "timestamp": "2023-11-26T02:28:26.30891403"
+    },
+    "trim_json": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-26T02:28:26.24768259"
+    },
+    "reads": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    [
+                        "test_1.fastp.fastq.gz:md5,67b2bbae47f073e05a97a9c2edce23c7",
+                        "test_2.fastp.fastq.gz:md5,25cbdca08e2083dbd4f0502de6b62f39"
+                    ]
+                ]
+            ]
+        ],
+        "timestamp": "2023-12-04T11:30:32.061644815"
+    },
+    "umi_log": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "timestamp": "2023-11-26T02:28:26.238536"
+    },
+    "trim_read_count": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    198
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-26T02:28:26.27984169"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/tags.yml b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/tags.yml
new file mode 100644
index 00000000..84a4b567
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/fastq_fastqc_umitools_fastp:
+  - subworkflows/nf-core/fastq_fastqc_umitools_fastp/**

From be1590056b38ad9744bd73d38f1fc45413693afd Mon Sep 17 00:00:00 2001
From: Fabian Hausmann <fabian.hausmann@zmnh.uni-hamburg.de>
Date: Thu, 11 Jan 2024 14:06:44 +0100
Subject: [PATCH 056/227] Add issue to changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 47cb7b72..a966783d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -5,7 +5,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## [dev](https://github.com/nf-core/smrnaseq/branch/dev)
 
-- _nothing yet done_
+- [[#299]](https://github.com/nf-core/smrnaseq/issues/299) - Bugfix for missing inputs in BAM stats (`genome_quant.r`)
 
 ## [v2.2.4](https://github.com/nf-core/smrnaseq/releases/tag/2.2.4) - 2023-11-03
 

From 1d4fd076d9a2e1382e847e42156196f6044f20fc Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 11 Jan 2024 13:07:25 +0000
Subject: [PATCH 057/227] Bringing this to 2024

---
 .github/CONTRIBUTING.md                       |   3 +
 .github/PULL_REQUEST_TEMPLATE.md              |   1 +
 .github/workflows/linting.yml                 |  12 +-
 assets/multiqc_config.yml                     |   4 +-
 lib/NfcoreTemplate.groovy                     |  32 +-
 modules.json                                  |  38 +--
 modules/nf-core/cat/fastq/environment.yml     |   1 +
 modules/nf-core/cat/fastq/main.nf             |   2 +-
 .../dumpsoftwareversions/environment.yml      |   3 +-
 .../custom/dumpsoftwareversions/main.nf       |   6 +-
 .../custom/dumpsoftwareversions/meta.yml      |   2 +-
 .../dumpsoftwareversions/tests/main.nf.test   |   7 +-
 .../tests/main.nf.test.snap                   |  50 +--
 modules/nf-core/fastqc/environment.yml        |   1 +
 modules/nf-core/fastqc/main.nf                |   6 +-
 modules/nf-core/fastqc/tests/main.nf.test     | 203 +++++++++++-
 .../nf-core/fastqc/tests/main.nf.test.snap    |  12 +-
 .../nf-core/samtools/bam2fq/environment.yml   |   7 +
 modules/nf-core/samtools/bam2fq/main.nf       |   6 +-
 modules/nf-core/samtools/bam2fq/meta.yml      |   8 +-
 .../samtools/bam2fq/tests/main.nf.test        |  71 ++++
 .../samtools/bam2fq/tests/main.nf.test.snap   |  49 +++
 .../samtools/bam2fq/tests/nextflow.config     |   3 +
 .../nf-core/samtools/bam2fq/tests/tags.yml    |   2 +
 .../nf-core/samtools/flagstat/environment.yml |   3 +-
 modules/nf-core/samtools/flagstat/main.nf     |   6 +-
 .../samtools/flagstat/tests/main.nf.test      |  36 +++
 .../samtools/flagstat/tests/main.nf.test.snap |  16 +
 .../nf-core/samtools/flagstat/tests/tags.yml  |   2 +
 .../nf-core/samtools/idxstats/environment.yml |   3 +-
 modules/nf-core/samtools/idxstats/main.nf     |   6 +-
 .../samtools/idxstats/tests/main.nf.test      |  36 +++
 .../samtools/idxstats/tests/main.nf.test.snap |  16 +
 .../nf-core/samtools/idxstats/tests/tags.yml  |   2 +
 .../nf-core/samtools/index/environment.yml    |   3 +-
 modules/nf-core/samtools/index/main.nf        |   6 +-
 .../samtools/index/tests/csi.nextflow.config  |   7 +
 .../nf-core/samtools/index/tests/main.nf.test |  87 +++++
 .../samtools/index/tests/main.nf.test.snap    |  28 ++
 modules/nf-core/samtools/index/tests/tags.yml |   2 +
 modules/nf-core/samtools/sort/environment.yml |   3 +-
 modules/nf-core/samtools/sort/main.nf         |   6 +-
 .../nf-core/samtools/sort/tests/main.nf.test  |   5 +-
 .../samtools/sort/tests/main.nf.test.snap     |  19 +-
 .../nf-core/samtools/stats/environment.yml    |   3 +-
 modules/nf-core/samtools/stats/main.nf        |   6 +-
 .../nf-core/samtools/stats/tests/main.nf.test |   2 +-
 .../samtools/stats/tests/main.nf.test.snap    |  20 +-
 .../nf-core/umitools/dedup/environment.yml    |   7 +
 modules/nf-core/umitools/dedup/main.nf        |  43 ++-
 modules/nf-core/umitools/dedup/meta.yml       |  82 +++--
 .../nf-core/umitools/extract/environment.yml  |   7 +
 modules/nf-core/umitools/extract/main.nf      |  13 +-
 modules/nf-core/umitools/extract/meta.yml     |  57 ++--
 .../umitools/extract/tests/main.nf.test       |  35 ++
 .../umitools/extract/tests/main.nf.test.snap  |  10 +
 .../umitools/extract/tests/nextflow.config    |   9 +
 .../nf-core/umitools/extract/tests/tags.yml   |   2 +
 .../tests/main.nf.test                        |  14 +-
 .../tests/main.nf.test.snap                   | 302 +++++-------------
 .../bam_sort_stats_samtools/tests/tags.yml    |   2 +-
 .../bam_stats_samtools/tests/main.nf.test     | 102 ++++++
 .../tests/main.nf.test.snap                   | 128 ++++++++
 .../nf-core/bam_stats_samtools/tests/tags.yml |   2 +
 64 files changed, 1225 insertions(+), 442 deletions(-)
 create mode 100644 modules/nf-core/samtools/bam2fq/environment.yml
 create mode 100644 modules/nf-core/samtools/bam2fq/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/bam2fq/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/bam2fq/tests/nextflow.config
 create mode 100644 modules/nf-core/samtools/bam2fq/tests/tags.yml
 create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/flagstat/tests/tags.yml
 create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/idxstats/tests/tags.yml
 create mode 100644 modules/nf-core/samtools/index/tests/csi.nextflow.config
 create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/index/tests/tags.yml
 create mode 100644 modules/nf-core/umitools/dedup/environment.yml
 create mode 100644 modules/nf-core/umitools/extract/environment.yml
 create mode 100644 modules/nf-core/umitools/extract/tests/main.nf.test
 create mode 100644 modules/nf-core/umitools/extract/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/umitools/extract/tests/nextflow.config
 create mode 100644 modules/nf-core/umitools/extract/tests/tags.yml
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/tags.yml

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 4d09370b..bd134c88 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -27,6 +27,9 @@ If you're not used to this workflow with git, you can start with some [docs from
 
 ## Tests
 
+You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to
+receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir <OUTDIR>`.
+
 When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests.
 Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then.
 
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index e278390b..3cdbf2b7 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -19,6 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/smrn
 - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
+- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
 - [ ] `CHANGELOG.md` is updated.
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index b8bdd214..905c58e4 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,9 +14,9 @@ jobs:
   EditorConfig:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
 
-      - uses: actions/setup-node@v3
+      - uses: actions/setup-node@v4
 
       - name: Install editorconfig-checker
         run: npm install -g editorconfig-checker
@@ -27,9 +27,9 @@ jobs:
   Prettier:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
 
-      - uses: actions/setup-node@v3
+      - uses: actions/setup-node@v4
 
       - name: Install Prettier
         run: npm install -g prettier
@@ -40,7 +40,7 @@ jobs:
   PythonBlack:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
 
       - name: Check code lints with Black
         uses: psf/black@stable
@@ -71,7 +71,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v3
+        uses: actions/checkout@v4
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v1
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 74c7de72..e953a059 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,7 +1,5 @@
 report_comment: >
-  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/releases/tag/dev" target="_blank">nf-core/smrnaseq</a>
-  analysis pipeline. For information about how to interpret these results, please see the
-  <a href="https://nf-co.re/smrnaseq/dev/docs/output" target="_blank">documentation</a>.
+  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/tree/dev" target="_blank">nf-core/smrnaseq</a> analysis pipeline. For information about how to interpret these results, please see the <a href="https://nf-co.re/smrnaseq/dev/docs/output" target="_blank">documentation</a>.
 report_section_order:
   "nf-core-smrnaseq-methods-description":
     order: -1000
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index 01b8653d..e248e4c3 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -4,6 +4,7 @@
 
 import org.yaml.snakeyaml.Yaml
 import groovy.json.JsonOutput
+import nextflow.extension.FilesEx
 
 class NfcoreTemplate {
 
@@ -141,12 +142,14 @@ class NfcoreTemplate {
             try {
                 if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
                 // Try to send HTML e-mail using sendmail
+                def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
+                sendmail_tf.withWriter { w -> w << sendmail_html }
                 [ 'sendmail', '-t' ].execute() << sendmail_html
                 log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
             } catch (all) {
                 // Catch failures and try with plaintext
                 def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
-                if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
+                if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
                     mail_cmd += [ '-A', mqc_report ]
                 }
                 mail_cmd.execute() << email_html
@@ -155,14 +158,16 @@ class NfcoreTemplate {
         }
 
         // Write summary e-mail HTML to a file
-        def output_d = new File("${params.outdir}/pipeline_info/")
-        if (!output_d.exists()) {
-            output_d.mkdirs()
-        }
-        def output_hf = new File(output_d, "pipeline_report.html")
+        def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
         output_hf.withWriter { w -> w << email_html }
-        def output_tf = new File(output_d, "pipeline_report.txt")
+        FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html");
+        output_hf.delete()
+
+        // Write summary e-mail TXT to a file
+        def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
         output_tf.withWriter { w -> w << email_txt }
+        FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt");
+        output_tf.delete()
     }
 
     //
@@ -227,15 +232,14 @@ class NfcoreTemplate {
     // Dump pipeline parameters in a json file
     //
     public static void dump_parameters(workflow, params) {
-        def output_d = new File("${params.outdir}/pipeline_info/")
-        if (!output_d.exists()) {
-            output_d.mkdirs()
-        }
-
         def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
-        def output_pf  = new File(output_d, "params_${timestamp}.json")
+        def filename   = "params_${timestamp}.json"
+        def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
         def jsonStr    = JsonOutput.toJson(params)
-        output_pf.text = JsonOutput.prettyPrint(jsonStr)
+        temp_pf.text   = JsonOutput.prettyPrint(jsonStr)
+
+        FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json")
+        temp_pf.delete()
     }
 
     //
diff --git a/modules.json b/modules.json
index 4a236584..56255242 100644
--- a/modules.json
+++ b/modules.json
@@ -12,12 +12,12 @@
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
+                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
                         "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
+                        "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
                         "installed_by": ["modules"]
                     },
                     "fastp": {
@@ -27,7 +27,7 @@
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
+                        "git_sha": "617777a807a1770f73deb38c80004bac06807eef",
                         "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "multiqc": {
@@ -37,42 +37,42 @@
                     },
                     "samtools/bam2fq": {
                         "branch": "master",
-                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905",
+                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
                         "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
-                        "installed_by": ["modules", "bam_stats_samtools"]
+                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
-                        "installed_by": ["modules", "bam_stats_samtools"]
+                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
-                        "installed_by": ["modules", "bam_sort_stats_samtools"]
+                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
-                        "installed_by": ["modules", "bam_sort_stats_samtools"]
+                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
-                        "git_sha": "516189e968feb4ebdd9921806988b4c12b4ac2dc",
-                        "installed_by": ["modules", "bam_stats_samtools"]
+                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "umitools/dedup": {
                         "branch": "master",
-                        "git_sha": "9d489648a7425fa18d8bed18935442c104d8733c",
+                        "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53",
                         "installed_by": ["modules"]
                     },
                     "umitools/extract": {
                         "branch": "master",
-                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905",
+                        "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53",
                         "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     }
                 }
@@ -81,13 +81,13 @@
                 "nf-core": {
                     "bam_sort_stats_samtools": {
                         "branch": "master",
-                        "git_sha": "7c8eeb2b37a6c6d3ffba0aef55ff60c8718c0ba6",
+                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
                         "installed_by": ["subworkflows"]
                     },
                     "bam_stats_samtools": {
                         "branch": "master",
-                        "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f",
-                        "installed_by": ["subworkflows", "bam_sort_stats_samtools"]
+                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "installed_by": ["bam_sort_stats_samtools", "subworkflows"]
                     },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
diff --git a/modules/nf-core/cat/fastq/environment.yml b/modules/nf-core/cat/fastq/environment.yml
index 222b301f..bff93add 100644
--- a/modules/nf-core/cat/fastq/environment.yml
+++ b/modules/nf-core/cat/fastq/environment.yml
@@ -1,3 +1,4 @@
+name: cat_fastq
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/cat/fastq/main.nf b/modules/nf-core/cat/fastq/main.nf
index b75a2e73..3d963784 100644
--- a/modules/nf-core/cat/fastq/main.nf
+++ b/modules/nf-core/cat/fastq/main.nf
@@ -2,7 +2,7 @@ process CAT_FASTQ {
     tag "$meta.id"
     label 'process_single'
 
-    conda 'modules/nf-core/cat/fastq/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
         'nf-core/ubuntu:20.04' }"
diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
index 7ca22161..9b3272bc 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
@@ -1,6 +1,7 @@
+name: custom_dumpsoftwareversions
 channels:
   - conda-forge
   - bioconda
   - defaults
 dependencies:
-  - bioconda::multiqc=1.15
+  - bioconda::multiqc=1.19
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index 60a19e0e..f2187611 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda 'modules/nf-core/custom/dumpsoftwareversions/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.15--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.19--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
index 9414c32d..5f15a5fd 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
@@ -1,4 +1,4 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
 name: custom_dumpsoftwareversions
 description: Custom module used to dump software versions within the nf-core pipeline template
 keywords:
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
index eec1db10..b1e1630b 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
+++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
@@ -31,7 +31,12 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                    process.out.versions,
+                    file(process.out.mqc_yml[0]).readLines()[0..10],
+                    file(process.out.yml[0]).readLines()[0..7]
+                    ).match()
+                }
             )
         }
     }
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
index 8713b921..5f59a936 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
+++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
@@ -1,27 +1,33 @@
 {
     "Should run without failures": {
         "content": [
-            {
-                "0": [
-                    "software_versions.yml:md5,a027f820f30b8191a20ca16465daaf37"
-                ],
-                "1": [
-                    "software_versions_mqc.yml:md5,ee4a1d028ad29987f9ac511f4668f17c"
-                ],
-                "2": [
-                    "versions.yml:md5,f47ebd22aba1dd987b7e5d5247b766c3"
-                ],
-                "mqc_yml": [
-                    "software_versions_mqc.yml:md5,ee4a1d028ad29987f9ac511f4668f17c"
-                ],
-                "versions": [
-                    "versions.yml:md5,f47ebd22aba1dd987b7e5d5247b766c3"
-                ],
-                "yml": [
-                    "software_versions.yml:md5,a027f820f30b8191a20ca16465daaf37"
-                ]
-            }
+            [
+                "versions.yml:md5,76d454d92244589d32455833f7c1ba6d"
+            ],
+            [
+                "data: \"<style>\\n#nf-core-versions tbody:nth-child(even) {\\n    background-color: #f2f2f2;\\n\\",
+                "  }\\n</style>\\n<table class=\\\"table\\\" style=\\\"width:100%\\\" id=\\\"nf-core-versions\\\"\\",
+                "  >\\n    <thead>\\n        <tr>\\n            <th> Process Name </th>\\n            <th>\\",
+                "  \\ Software </th>\\n            <th> Version  </th>\\n        </tr>\\n    </thead>\\n\\",
+                "  \\n<tbody>\\n<tr>\\n    <td><samp>CUSTOM_DUMPSOFTWAREVERSIONS</samp></td>\\n    <td><samp>python</samp></td>\\n\\",
+                "  \\    <td><samp>3.11.7</samp></td>\\n</tr>\\n\\n<tr>\\n    <td><samp></samp></td>\\n \\",
+                "  \\   <td><samp>yaml</samp></td>\\n    <td><samp>5.4.1</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
+                "  <tbody>\\n<tr>\\n    <td><samp>TOOL1</samp></td>\\n    <td><samp>tool1</samp></td>\\n\\",
+                "  \\    <td><samp>0.11.9</samp></td>\\n</tr>\\n\\n</tbody>\\n<tbody>\\n<tr>\\n    <td><samp>TOOL2</samp></td>\\n\\",
+                "  \\    <td><samp>tool2</samp></td>\\n    <td><samp>1.9</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
+                "  <tbody>\\n<tr>\\n    <td><samp>Workflow</samp></td>\\n    <td><samp>Nextflow</samp></td>\\n\\"
+            ],
+            [
+                "CUSTOM_DUMPSOFTWAREVERSIONS:",
+                "  python: 3.11.7",
+                "  yaml: 5.4.1",
+                "TOOL1:",
+                "  tool1: 0.11.9",
+                "TOOL2:",
+                "  tool2: '1.9'",
+                "Workflow:"
+            ]
         ],
-        "timestamp": "2023-10-11T17:10:02.930699"
+        "timestamp": "2024-01-09T23:01:18.710682"
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml
index f52a53a0..1787b38a 100644
--- a/modules/nf-core/fastqc/environment.yml
+++ b/modules/nf-core/fastqc/environment.yml
@@ -1,3 +1,4 @@
+name: fastqc
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 5def8818..9e19a74c 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -2,7 +2,7 @@ process FASTQC {
     tag "$meta.id"
     label 'process_medium'
 
-    conda 'modules/nf-core/fastqc/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' :
         'biocontainers/fastqc:0.12.1--hdfd78af_0' }"
@@ -37,7 +37,7 @@ process FASTQC {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' )
     END_VERSIONS
     """
 
@@ -49,7 +49,7 @@ process FASTQC {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' )
     END_VERSIONS
     """
 }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
index 6437a144..ad9bc54f 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -3,23 +3,21 @@ nextflow_process {
     name "Test Process FASTQC"
     script "../main.nf"
     process "FASTQC"
+
     tag "modules"
     tag "modules_nfcore"
     tag "fastqc"
 
-    test("Single-Read") {
+    test("sarscov2 single-end [fastq]") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = [
-                    [ id: 'test', single_end:true ],
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-                    ]
+                            [ id: 'test', single_end:true ],
+                            [
+                                file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                            ]
                 ]
                 """
             }
@@ -28,14 +26,195 @@ nextflow_process {
         then {
             assertAll (
             { assert process.success },
+
             // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
             // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
             // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
-            { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" },
-            { assert path(process.out.html.get(0).get(1)).getText().contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert snapshot(process.out.versions).match("versions") },
-            { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" }
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq]") {
+
+        when {
+            process {
+            """
+            input[0] = [
+                        [id: 'test', single_end: false], // meta map
+                        [
+                            file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                        ]
+            ]
+            """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 interleaved [fastq]") {
+
+        when {
+            process {
+            """
+            input[0] = [
+                        [id: 'test', single_end: false], // meta map
+                        file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true)
+            ]
+            """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [bam]") {
+
+        when {
+            process {
+            """
+            input[0] = [
+                        [id: 'test', single_end: false], // meta map
+                        file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
+            ]
+            """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
             )
         }
     }
+
+    test("sarscov2 multiple [fastq]") {
+
+        when {
+            process {
+            """
+            input[0] = [
+                        [id: 'test', single_end: false], // meta map
+                        [
+                            file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true),
+                            file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true)
+                        ]
+            ]
+            """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+            { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" },
+            { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" },
+            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+            { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" },
+            { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" },
+            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 custom_prefix") {
+
+        when {
+            process {
+            """
+            input[0] = [
+                        [ id:'mysample', single_end:true ], // meta map
+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+            ]
+            """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastq] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id: 'test', single_end:true ],
+                            [
+                                file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                            ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out.html.collect { file(it[1]).getName() } +
+                                process.out.zip.collect { file(it[1]).getName() } +
+                                process.out.versions ).match() }
+            )
+        }
+    }
+
 }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
index 636a32ce..5ef5afbd 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test.snap
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -1,10 +1,20 @@
 {
+    "sarscov2 single-end [fastq] - stub": {
+        "content": [
+            [
+                "test.html",
+                "test.zip",
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "timestamp": "2023-12-29T02:48:05.126117287"
+    },
     "versions": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
-        "timestamp": "2023-10-09T23:40:54+0000"
+        "timestamp": "2023-12-29T02:46:49.507942667"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/bam2fq/environment.yml b/modules/nf-core/samtools/bam2fq/environment.yml
new file mode 100644
index 00000000..5297496f
--- /dev/null
+++ b/modules/nf-core/samtools/bam2fq/environment.yml
@@ -0,0 +1,7 @@
+name: samtools_bam2fq
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::samtools=1.18
diff --git a/modules/nf-core/samtools/bam2fq/main.nf b/modules/nf-core/samtools/bam2fq/main.nf
index 9301d1d3..55ffd0cf 100644
--- a/modules/nf-core/samtools/bam2fq/main.nf
+++ b/modules/nf-core/samtools/bam2fq/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_BAM2FQ {
     tag "$meta.id"
     label 'process_low'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
-        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
+        'biocontainers/samtools:1.18--h50ea8bc_1' }"
 
     input:
     tuple val(meta), path(inputbam)
diff --git a/modules/nf-core/samtools/bam2fq/meta.yml b/modules/nf-core/samtools/bam2fq/meta.yml
index 319a60cf..7769046b 100644
--- a/modules/nf-core/samtools/bam2fq/meta.yml
+++ b/modules/nf-core/samtools/bam2fq/meta.yml
@@ -9,12 +9,8 @@ keywords:
 tools:
   - samtools:
       description: Tools for dealing with SAM, BAM and CRAM files
-      homepage: None
       documentation: http://www.htslib.org/doc/1.1/samtools.html
-      tool_dev_url: None
-      doi: ""
       licence: ["MIT"]
-
 input:
   - meta:
       type: map
@@ -33,7 +29,6 @@ input:
         Note: choosing TRUE will generate 4 different files.
         Choosing FALSE will produce a single file, which will be interleaved in case
         the input contains paired reads.
-
 output:
   - meta:
       type: map
@@ -50,6 +45,7 @@ output:
         FASTQ files, which will be either a group of 4 files (read_1, read_2, other and singleton)
         or a single interleaved .fq.gz file if the user chooses not to split the reads.
       pattern: "*.fq.gz"
-
 authors:
   - "@lescai"
+maintainers:
+  - "@lescai"
diff --git a/modules/nf-core/samtools/bam2fq/tests/main.nf.test b/modules/nf-core/samtools/bam2fq/tests/main.nf.test
new file mode 100644
index 00000000..cd65abbe
--- /dev/null
+++ b/modules/nf-core/samtools/bam2fq/tests/main.nf.test
@@ -0,0 +1,71 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_BAM2FQ"
+    script "../main.nf"
+    process "SAMTOOLS_BAM2FQ"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/bam2fq"
+
+    config "./nextflow.config"
+
+    test("homo_sapiens - bam, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.test_data['homo_sapiens']['illumina']['test_paired_end_umi_converted_bam'], checkIfExists: true)
+                ]
+                input[1] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    path(process.out.reads[0][1]).linesGzip[0..6],
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - bam, true") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.test_data['homo_sapiens']['illumina']['test_paired_end_umi_converted_bam'], checkIfExists: true)
+                ]
+                input[1] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.reads[0][1].collect{
+                        if(it ==~ /.*(other|singleton)\.fq\.gz$/) {
+                            return file(it).name
+                        }
+                        return path(it).linesGzip[0..6]
+                    },
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/samtools/bam2fq/tests/main.nf.test.snap b/modules/nf-core/samtools/bam2fq/tests/main.nf.test.snap
new file mode 100644
index 00000000..1f824503
--- /dev/null
+++ b/modules/nf-core/samtools/bam2fq/tests/main.nf.test.snap
@@ -0,0 +1,49 @@
+{
+    "homo_sapiens - bam, false": {
+        "content": [
+            [
+                "@922332/1\tRX:Z:ATTTCAG-TATTATT",
+                "GAGAGGATCTCGTGTAGAAATTGCTTTGAGCTGTTCTTTGTCATTTTCCCTTAATTCATTGTCTCTAGCTAGTCTGTTACTCTGTAAAATAAAATAATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTG",
+                "+",
+                "EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEEAEAEE6AAEEEEE/EAAAA<AEEEEAAEEAAAA<EEE/",
+                "@922332/2\tRX:Z:ATTTCAG-TATTATT",
+                "TATTTTACAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTCTCTGAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGAT",
+                "+"
+            ],
+            [
+                "versions.yml:md5,0b88ba6888f9730c167242c0c4b3d04b"
+            ]
+        ],
+        "timestamp": "2023-12-04T13:40:52.124056991"
+    },
+    "homo_sapiens - bam, true": {
+        "content": [
+            [
+                [
+                    "@922332\tRX:Z:ATTTCAG-TATTATT",
+                    "GAGAGGATCTCGTGTAGAAATTGCTTTGAGCTGTTCTTTGTCATTTTCCCTTAATTCATTGTCTCTAGCTAGTCTGTTACTCTGTAAAATAAAATAATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTG",
+                    "+",
+                    "EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEEAEAEE6AAEEEEE/EAAAA<AEEEEAAEEAAAA<EEE/",
+                    "@928177\tRX:Z:ACATAAA-TGAGATT",
+                    "AAAAGTATATAAGTAATACATATTTATAAATCTATTAAGAAAGCAAGTAATATGTACCTTAAGAATTTAATGGGAAAATAATTAGACTTACTTTAAATGCCAAAAGAAAAAGTGCCCAATCCTTTGATTAGTCAATGCTTTCT",
+                    "+"
+                ],
+                [
+                    "@922332\tRX:Z:ATTTCAG-TATTATT",
+                    "TATTTTACAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTCTCTGAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGAT",
+                    "+",
+                    "EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEA<AEEE<<<<AAEEEEEEEEEEA<EEEAEAE//A<AAE<6",
+                    "@928177\tRX:Z:ACATAAA-TGAGATT",
+                    "TTACTGAAGAAAGCATTGACTAATCAAAGGATTGGGCACTTTTTCTTTTGGCATTTAAAGTAAGTCTAATTATTTTCCCATTAAATTCTTAAGGTACATATTACTTGCTTTCTTAATAGATTTATAAATATGTATTACTTATA",
+                    "+"
+                ],
+                "test_other.fq.gz",
+                "test_singleton.fq.gz"
+            ],
+            [
+                "versions.yml:md5,0b88ba6888f9730c167242c0c4b3d04b"
+            ]
+        ],
+        "timestamp": "2023-12-04T13:45:59.949456034"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/bam2fq/tests/nextflow.config b/modules/nf-core/samtools/bam2fq/tests/nextflow.config
new file mode 100644
index 00000000..dae4e48b
--- /dev/null
+++ b/modules/nf-core/samtools/bam2fq/tests/nextflow.config
@@ -0,0 +1,3 @@
+process {
+    ext.args = '-T RX'
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/bam2fq/tests/tags.yml b/modules/nf-core/samtools/bam2fq/tests/tags.yml
new file mode 100644
index 00000000..2fbf4a1f
--- /dev/null
+++ b/modules/nf-core/samtools/bam2fq/tests/tags.yml
@@ -0,0 +1,2 @@
+samtools/bam2fq:
+  - "modules/nf-core/samtools/bam2fq/**"
diff --git a/modules/nf-core/samtools/flagstat/environment.yml b/modules/nf-core/samtools/flagstat/environment.yml
index 04c82f14..5efae053 100644
--- a/modules/nf-core/samtools/flagstat/environment.yml
+++ b/modules/nf-core/samtools/flagstat/environment.yml
@@ -1,6 +1,7 @@
+name: samtools_flagstat
 channels:
   - conda-forge
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.17
+  - bioconda::samtools=1.18
diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf
index b289d151..f1893d7c 100644
--- a/modules/nf-core/samtools/flagstat/main.nf
+++ b/modules/nf-core/samtools/flagstat/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_FLAGSTAT {
     tag "$meta.id"
     label 'process_single'
 
-    conda 'modules/nf-core/samtools/flagstat/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
+        'biocontainers/samtools:1.18--h50ea8bc_1' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test b/modules/nf-core/samtools/flagstat/tests/main.nf.test
new file mode 100644
index 00000000..c8dd8dc9
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test
@@ -0,0 +1,36 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_FLAGSTAT"
+    script "../main.nf"
+    process "SAMTOOLS_FLAGSTAT"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/flagstat"
+
+    test("BAM") {
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
+                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out.flagstat).match() },
+                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
new file mode 100644
index 00000000..880019f2
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
@@ -0,0 +1,16 @@
+{
+    "BAM": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-14T15:49:22.577133"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/flagstat/tests/tags.yml b/modules/nf-core/samtools/flagstat/tests/tags.yml
new file mode 100644
index 00000000..2d2b7255
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/tests/tags.yml
@@ -0,0 +1,2 @@
+samtools/flagstat:
+  - modules/nf-core/samtools/flagstat/**
diff --git a/modules/nf-core/samtools/idxstats/environment.yml b/modules/nf-core/samtools/idxstats/environment.yml
index 04c82f14..2401db0f 100644
--- a/modules/nf-core/samtools/idxstats/environment.yml
+++ b/modules/nf-core/samtools/idxstats/environment.yml
@@ -1,6 +1,7 @@
+name: samtools_idxstats
 channels:
   - conda-forge
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.17
+  - bioconda::samtools=1.18
diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf
index 97217419..00d916bb 100644
--- a/modules/nf-core/samtools/idxstats/main.nf
+++ b/modules/nf-core/samtools/idxstats/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_IDXSTATS {
     tag "$meta.id"
     label 'process_single'
 
-    conda 'modules/nf-core/samtools/idxstats/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
+        'biocontainers/samtools:1.18--h50ea8bc_1' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test b/modules/nf-core/samtools/idxstats/tests/main.nf.test
new file mode 100644
index 00000000..f6c92150
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test
@@ -0,0 +1,36 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_IDXSTATS"
+    script "../main.nf"
+    process "SAMTOOLS_IDXSTATS"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/idxstats"
+
+    test("BAM") {
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
+                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out.idxstats).match() },
+                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
new file mode 100644
index 00000000..4c6c12bd
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
@@ -0,0 +1,16 @@
+{
+    "BAM": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-14T15:52:19.875194"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/idxstats/tests/tags.yml b/modules/nf-core/samtools/idxstats/tests/tags.yml
new file mode 100644
index 00000000..d3057c61
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/tests/tags.yml
@@ -0,0 +1,2 @@
+samtools/idxstats:
+  - modules/nf-core/samtools/idxstats/**
diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml
index 04c82f14..296ed99e 100644
--- a/modules/nf-core/samtools/index/environment.yml
+++ b/modules/nf-core/samtools/index/environment.yml
@@ -1,6 +1,7 @@
+name: samtools_index
 channels:
   - conda-forge
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.17
+  - bioconda::samtools=1.18
diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf
index af3dbc4c..8ad18fdc 100644
--- a/modules/nf-core/samtools/index/main.nf
+++ b/modules/nf-core/samtools/index/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_INDEX {
     tag "$meta.id"
     label 'process_low'
 
-    conda 'modules/nf-core/samtools/index/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
+        'biocontainers/samtools:1.18--h50ea8bc_1' }"
 
     input:
     tuple val(meta), path(input)
diff --git a/modules/nf-core/samtools/index/tests/csi.nextflow.config b/modules/nf-core/samtools/index/tests/csi.nextflow.config
new file mode 100644
index 00000000..0ed260ef
--- /dev/null
+++ b/modules/nf-core/samtools/index/tests/csi.nextflow.config
@@ -0,0 +1,7 @@
+process {
+
+    withName: SAMTOOLS_INDEX {
+        ext.args = '-c'
+    }
+
+}
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test
new file mode 100644
index 00000000..c76a9169
--- /dev/null
+++ b/modules/nf-core/samtools/index/tests/main.nf.test
@@ -0,0 +1,87 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_INDEX"
+    script "../main.nf"
+    process "SAMTOOLS_INDEX"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/index"
+
+    test("sarscov2 [BAI]") {
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out.bai).match("bai") },
+                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+            )
+        }
+    }
+
+    test("homo_sapiens [CRAI]") {
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out.crai).match("crai") },
+                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+            )
+        }
+    }
+
+    test("homo_sapiens [CSI]") {
+
+        config "./csi.nextflow.config"
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert path(process.out.csi.get(0).get(1)).exists() },
+                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap
new file mode 100644
index 00000000..b3baee7f
--- /dev/null
+++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap
@@ -0,0 +1,28 @@
+{
+    "crai": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-15T15:17:37.30801"
+    },
+    "bai": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-15T15:17:30.869234"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/index/tests/tags.yml b/modules/nf-core/samtools/index/tests/tags.yml
new file mode 100644
index 00000000..e0f58a7a
--- /dev/null
+++ b/modules/nf-core/samtools/index/tests/tags.yml
@@ -0,0 +1,2 @@
+samtools/index:
+  - modules/nf-core/samtools/index/**
diff --git a/modules/nf-core/samtools/sort/environment.yml b/modules/nf-core/samtools/sort/environment.yml
index 04c82f14..cd50868c 100644
--- a/modules/nf-core/samtools/sort/environment.yml
+++ b/modules/nf-core/samtools/sort/environment.yml
@@ -1,6 +1,7 @@
+name: samtools_sort
 channels:
   - conda-forge
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.17
+  - bioconda::samtools=1.18
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index 77256702..4a666d42 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_SORT {
     tag "$meta.id"
     label 'process_medium'
 
-    conda 'modules/nf-core/samtools/sort/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
+        'biocontainers/samtools:1.18--h50ea8bc_1' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test
index 1f72f3b9..abb80978 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test
@@ -61,7 +61,10 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    process.out.versions
+                ).match() }
             )
         }
 
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
index a43566da..ff722259 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
@@ -8,14 +8,14 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,a29570e7607d217c2fa4d75829e09cd7"
+                        "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06"
                     ]
                 ],
                 "1": [
                     
                 ],
                 "2": [
-                    "versions.yml:md5,46f7a36082fa1f68285fe30d689244e8"
+                    "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80"
                 ],
                 "bam": [
                     [
@@ -23,17 +23,26 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,a29570e7607d217c2fa4d75829e09cd7"
+                        "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06"
                     ]
                 ],
                 "csi": [
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,46f7a36082fa1f68285fe30d689244e8"
+                    "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80"
                 ]
             }
         ],
-        "timestamp": "2023-10-17T17:21:46.5427968"
+        "timestamp": "2023-12-04T11:11:22.005628301"
+    },
+    "test_samtools_sort_stub": {
+        "content": [
+            "test.sorted.bam",
+            [
+                "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80"
+            ]
+        ],
+        "timestamp": "2023-12-04T17:47:22.314445935"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml
index 04c82f14..b89ce647 100644
--- a/modules/nf-core/samtools/stats/environment.yml
+++ b/modules/nf-core/samtools/stats/environment.yml
@@ -1,6 +1,7 @@
+name: samtools_stats
 channels:
   - conda-forge
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.17
+  - bioconda::samtools=1.18
diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf
index fe30bf89..7539140a 100644
--- a/modules/nf-core/samtools/stats/main.nf
+++ b/modules/nf-core/samtools/stats/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_STATS {
     tag "$meta.id"
     label 'process_single'
 
-    conda 'modules/nf-core/samtools/stats/environment.yml'
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
+        'biocontainers/samtools:1.18--h50ea8bc_1' }"
 
     input:
     tuple val(meta), path(input), path(input_index)
diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test b/modules/nf-core/samtools/stats/tests/main.nf.test
index e037132c..20c3efe1 100644
--- a/modules/nf-core/samtools/stats/tests/main.nf.test
+++ b/modules/nf-core/samtools/stats/tests/main.nf.test
@@ -4,7 +4,7 @@ nextflow_process {
     script "../main.nf"
     process "SAMTOOLS_STATS"
     tag "modules"
-    tag "modules/nf-core"
+    tag "modules_nfcore"
     tag "samtools"
     tag "samtools/stats"
 
diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test.snap b/modules/nf-core/samtools/stats/tests/main.nf.test.snap
index 516b2b01..025c83a5 100644
--- a/modules/nf-core/samtools/stats/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/stats/tests/main.nf.test.snap
@@ -8,11 +8,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.stats:md5,6e768486d5df0257351c5419a79f9c9b"
+                        "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def"
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,08035f3409d934d47a416150884bb0df"
+                    "versions.yml:md5,650a365c6635001436008350ae83337c"
                 ],
                 "stats": [
                     [
@@ -20,15 +20,15 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.stats:md5,6e768486d5df0257351c5419a79f9c9b"
+                        "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def"
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,08035f3409d934d47a416150884bb0df"
+                    "versions.yml:md5,650a365c6635001436008350ae83337c"
                 ]
             }
         ],
-        "timestamp": "2023-10-18T12:12:42.998746"
+        "timestamp": "2023-12-04T11:07:28.26821485"
     },
     "SAMTOOLS CRAM Should run without failures": {
         "content": [
@@ -39,11 +39,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.stats:md5,7c9ee5747793cceb9d6f4d733345641a"
+                        "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43"
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,08035f3409d934d47a416150884bb0df"
+                    "versions.yml:md5,650a365c6635001436008350ae83337c"
                 ],
                 "stats": [
                     [
@@ -51,14 +51,14 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.stats:md5,7c9ee5747793cceb9d6f4d733345641a"
+                        "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43"
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,08035f3409d934d47a416150884bb0df"
+                    "versions.yml:md5,650a365c6635001436008350ae83337c"
                 ]
             }
         ],
-        "timestamp": "2023-10-18T12:13:30.747222"
+        "timestamp": "2023-12-04T11:07:50.356233402"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/umitools/dedup/environment.yml b/modules/nf-core/umitools/dedup/environment.yml
new file mode 100644
index 00000000..f443735f
--- /dev/null
+++ b/modules/nf-core/umitools/dedup/environment.yml
@@ -0,0 +1,7 @@
+name: umitools_dedup
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::umi_tools=1.1.4
diff --git a/modules/nf-core/umitools/dedup/main.nf b/modules/nf-core/umitools/dedup/main.nf
index dfcbcf2f..64ab8f98 100644
--- a/modules/nf-core/umitools/dedup/main.nf
+++ b/modules/nf-core/umitools/dedup/main.nf
@@ -2,19 +2,21 @@ process UMITOOLS_DEDUP {
     tag "$meta.id"
     label "process_medium"
 
-    conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
-        'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
+        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.4--py38hbff2b2d_1' :
+        'biocontainers/umi_tools:1.1.4--py38hbff2b2d_1' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
+    val get_output_stats
 
     output:
-    tuple val(meta), path("*.bam")             , emit: bam
-    tuple val(meta), path("*edit_distance.tsv"), emit: tsv_edit_distance
-    tuple val(meta), path("*per_umi.tsv")      , emit: tsv_per_umi
-    tuple val(meta), path("*per_position.tsv") , emit: tsv_umi_per_position
+    tuple val(meta), path("${prefix}.bam")     , emit: bam
+    tuple val(meta), path("*.log")             , emit: log
+    tuple val(meta), path("*edit_distance.tsv"), optional:true, emit: tsv_edit_distance
+    tuple val(meta), path("*per_umi.tsv")      , optional:true, emit: tsv_per_umi
+    tuple val(meta), path("*per_position.tsv") , optional:true, emit: tsv_umi_per_position
     path  "versions.yml"                       , emit: versions
 
     when:
@@ -22,20 +24,39 @@ process UMITOOLS_DEDUP {
 
     script:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     def paired = meta.single_end ? "" : "--paired"
+    stats = get_output_stats ? "--output-stats ${prefix}" : ""
+    if ("$bam" == "${prefix}.bam") error "Input and output names are the same, set prefix in module configuration to disambiguate!"
+
+    if (!(args ==~ /.*--random-seed.*/)) {args += " --random-seed=100"}
     """
-    umi_tools \\
+    PYTHONHASHSEED=0 umi_tools \\
         dedup \\
         -I $bam \\
         -S ${prefix}.bam \\
-        --output-stats $prefix \\
+        -L ${prefix}.log \\
+        $stats \\
         $paired \\
         $args
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
+        umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' )
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch ${prefix}.bam
+    touch ${prefix}.log
+    touch ${prefix}_edit_distance.tsv
+    touch ${prefix}_per_umi.tsv
+    touch ${prefix}_per_position.tsv
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' )
     END_VERSIONS
     """
 }
diff --git a/modules/nf-core/umitools/dedup/meta.yml b/modules/nf-core/umitools/dedup/meta.yml
index eee8952f..38d3fd46 100644
--- a/modules/nf-core/umitools/dedup/meta.yml
+++ b/modules/nf-core/umitools/dedup/meta.yml
@@ -3,57 +3,69 @@ description: Deduplicate reads based on the mapping co-ordinate and the UMI atta
 keywords:
   - umitools
   - deduplication
+  - dedup
 tools:
   - umi_tools:
-    description: >
-      UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs)
-      and single cell RNA-Seq cell barcodes
-    documentation: https://umi-tools.readthedocs.io/en/latest/
-    license: ["MIT"]
+      description: >
+        UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes
+
+      documentation: https://umi-tools.readthedocs.io/en/latest/
+      license: ["MIT"]
 input:
   - meta:
-    type: map
-    description: |
-      Groovy Map containing sample information
+      type: map
+      description: |
+        Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
   - bam:
-    type: file
-    description: |
-      BAM file containing reads to be deduplicated via UMIs.
-    pattern: "*.{bam}"
+      type: file
+      description: |
+        BAM file containing reads to be deduplicated via UMIs.
+      pattern: "*.{bam}"
   - bai:
-    type: file
-    description: |
-      BAM index files corresponding to the input BAM file.
-    pattern: "*.{bai}"
+      type: file
+      description: |
+        BAM index files corresponding to the input BAM file.
+      pattern: "*.{bai}"
+  - get_output_stats:
+      type: boolean
+      description: |
+        Whether or not to generate output stats.
 output:
   - meta:
-    type: map
-    description: |
-      Groovy Map containing sample information
+      type: map
+      description: |
+        Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
   - bam:
-    type: file
-    description: BAM file with deduplicated UMIs.
-    pattern: "*.{bam}"
+      type: file
+      description: BAM file with deduplicated UMIs.
+      pattern: "*.{bam}"
+  - log:
+      type: file
+      description: File with logging information
+      pattern: "*.{log}"
   - tsv_edit_distance:
-    type: file
-    description: Reports the (binned) average edit distance between the UMIs at each position.
-    pattern: "*edit_distance.tsv"
+      type: file
+      description: Reports the (binned) average edit distance between the UMIs at each position.
+      pattern: "*edit_distance.tsv"
   - tsv_per_umi:
-    type: file
-    description: UMI-level summary statistics.
-    pattern: "*per_umi.tsv"
+      type: file
+      description: UMI-level summary statistics.
+      pattern: "*per_umi.tsv"
   - tsv_umi_per_position:
-    type: file
-    description: Tabulates the counts for unique combinations of UMI and position.
-    pattern: "*per_position.tsv"
+      type: file
+      description: Tabulates the counts for unique combinations of UMI and position.
+      pattern: "*per_position.tsv"
   - versions:
-    type: file
-    description: File containing software versions
-    pattern: "versions.yml"
-
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@grst"
   - "@klkeys"
+maintainers:
+  - "@drpatelh"
+  - "@grst"
+  - "@klkeys"
diff --git a/modules/nf-core/umitools/extract/environment.yml b/modules/nf-core/umitools/extract/environment.yml
new file mode 100644
index 00000000..7d08ac0e
--- /dev/null
+++ b/modules/nf-core/umitools/extract/environment.yml
@@ -0,0 +1,7 @@
+name: umitools_extract
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::umi_tools=1.1.4
diff --git a/modules/nf-core/umitools/extract/main.nf b/modules/nf-core/umitools/extract/main.nf
index 22a405b9..4bd79e79 100644
--- a/modules/nf-core/umitools/extract/main.nf
+++ b/modules/nf-core/umitools/extract/main.nf
@@ -1,11 +1,12 @@
 process UMITOOLS_EXTRACT {
     tag "$meta.id"
-    label "process_low"
+    label "process_single"
+    label "process_long"
 
-    conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
-        'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
+        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.4--py38hbff2b2d_1' :
+        'biocontainers/umi_tools:1.1.4--py38hbff2b2d_1' }"
 
     input:
     tuple val(meta), path(reads)
@@ -32,7 +33,7 @@ process UMITOOLS_EXTRACT {
 
         cat <<-END_VERSIONS > versions.yml
         "${task.process}":
-            umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
+            umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' )
         END_VERSIONS
         """
     }  else {
@@ -48,7 +49,7 @@ process UMITOOLS_EXTRACT {
 
         cat <<-END_VERSIONS > versions.yml
         "${task.process}":
-            umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
+            umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' )
         END_VERSIONS
         """
     }
diff --git a/modules/nf-core/umitools/extract/meta.yml b/modules/nf-core/umitools/extract/meta.yml
index 7fc23f72..7695b271 100644
--- a/modules/nf-core/umitools/extract/meta.yml
+++ b/modules/nf-core/umitools/extract/meta.yml
@@ -1,47 +1,48 @@
 name: umitools_extract
 description: Extracts UMI barcode from a read and add it to the read name, leaving any sample barcode in place
 keywords:
-  - umitools
+  - UMI
+  - barcode
   - extract
+  - umitools
 tools:
   - umi_tools:
-    description: >
-      UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs)
-      and single cell RNA-Seq cell barcodes
-    documentation: https://umi-tools.readthedocs.io/en/latest/
-    license: ["MIT"]
+      description: >
+        UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes
+      documentation: https://umi-tools.readthedocs.io/en/latest/
+      license: "MIT"
 input:
   - meta:
-    type: map
-    description: |
-      Groovy Map containing sample information
+      type: map
+      description: |
+        Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
   - reads:
-    type: list
-    description: |
-      List of input FASTQ files whose UMIs will be extracted.
+      type: list
+      description: |
+        List of input FASTQ files whose UMIs will be extracted.
 output:
   - meta:
-    type: map
-    description: |
-      Groovy Map containing sample information
+      type: map
+      description: |
+        Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
   - reads:
-    type: file
-    description: >
-      Extracted FASTQ files. |
-      For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. |
-        For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
-    pattern: "*.{fastq.gz}"
+      type: file
+      description: >
+        Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
+      pattern: "*.{fastq.gz}"
   - log:
-    type: file
-    description: Logfile for umi_tools
-    pattern: "*.{log}"
+      type: file
+      description: Logfile for umi_tools
+      pattern: "*.{log}"
   - versions:
-    type: file
-    description: File containing software versions
-    pattern: "versions.yml"
-
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@grst"
+maintainers:
+  - "@drpatelh"
+  - "@grst"
diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test b/modules/nf-core/umitools/extract/tests/main.nf.test
new file mode 100644
index 00000000..22242d1d
--- /dev/null
+++ b/modules/nf-core/umitools/extract/tests/main.nf.test
@@ -0,0 +1,35 @@
+nextflow_process {
+
+    name "Test Process UMITOOLS_EXTRACT"
+    script "../main.nf"
+    process "UMITOOLS_EXTRACT"
+    config "./nextflow.config"
+    tag "modules_nfcore"
+    tag "modules"
+    tag "umitools"
+    tag "umitools/extract"
+
+    test("Should run without failures") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:true ], // meta map
+              [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
+            ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test.snap b/modules/nf-core/umitools/extract/tests/main.nf.test.snap
new file mode 100644
index 00000000..6d5944f1
--- /dev/null
+++ b/modules/nf-core/umitools/extract/tests/main.nf.test.snap
@@ -0,0 +1,10 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,5a18da2d3a5a4de15e7aaae9082d7abb"
+            ]
+        ],
+        "timestamp": "2023-12-08T09:41:43.540658352"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/umitools/extract/tests/nextflow.config b/modules/nf-core/umitools/extract/tests/nextflow.config
new file mode 100644
index 00000000..c866f5a0
--- /dev/null
+++ b/modules/nf-core/umitools/extract/tests/nextflow.config
@@ -0,0 +1,9 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+    
+    withName: UMITOOLS_EXTRACT {
+        ext.args = '--bc-pattern="NNNN"'
+    }
+
+}
diff --git a/modules/nf-core/umitools/extract/tests/tags.yml b/modules/nf-core/umitools/extract/tests/tags.yml
new file mode 100644
index 00000000..c3fb23de
--- /dev/null
+++ b/modules/nf-core/umitools/extract/tests/tags.yml
@@ -0,0 +1,2 @@
+umitools/extract:
+  - modules/nf-core/umitools/extract/**
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test
index a8a13f2a..59b749d8 100644
--- a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test
@@ -5,7 +5,9 @@ nextflow_workflow {
     workflow "BAM_SORT_STATS_SAMTOOLS"
     tag "subworkflows"
     tag "subworkflows_nfcore"
+    tag "subworkflows/bam_sort_stats_samtools"
     tag "bam_sort_stats_samtools"
+    tag "subworkflows/bam_stats_samtools"
     tag "bam_stats_samtools"
     tag "samtools"
     tag "samtools/index"
@@ -35,7 +37,11 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success},
-                { assert snapshot(workflow.out).match()}
+                { assert workflow.out.bam.get(0).get(1) ==~ ".*.bam"},
+                { assert workflow.out.bai.get(0).get(1) ==~ ".*.bai"},
+                { assert snapshot(workflow.out.stats).match("test_bam_sort_stats_samtools_single_end_stats") },
+                { assert snapshot(workflow.out.flagstat).match("test_bam_sort_stats_samtools_single_end_flagstats") },
+                { assert snapshot(workflow.out.idxstats).match("test_bam_sort_stats_samtools_single_end_idxstats") }
             )
         }
     }
@@ -61,7 +67,11 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success},
-                { assert snapshot(workflow.out).match()}
+                { assert workflow.out.bam.get(0).get(1) ==~ ".*.bam"},
+                { assert workflow.out.bai.get(0).get(1) ==~ ".*.bai"},
+                { assert snapshot(workflow.out.stats).match("test_bam_sort_stats_samtools_paired_end_stats") },
+                { assert snapshot(workflow.out.flagstat).match("test_bam_sort_stats_samtools_paired_end_flagstats") },
+                { assert snapshot(workflow.out.idxstats).match("test_bam_sort_stats_samtools_paired_end_idxstats") }
             )
         }
     }
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
index 50ffde60..77afbf17 100644
--- a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
@@ -1,236 +1,86 @@
 {
-    "test_bam_sort_stats_samtools_single_end": {
+    "test_bam_sort_stats_samtools_paired_end_flagstats": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,2cf8fe8dbba3da7eb4fb251c79f428dc"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam.bai:md5,002488588110dcee464e65f68c4726e8"
-                    ]
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.stats:md5,796f45f791f06291b76329528fae0a54"
-                    ]
-                ],
-                "4": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.flagstat:md5,2191911d72575a2358b08b1df64ccb53"
-                    ]
-                ],
-                "5": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.idxstats:md5,613e048487662c694aa4a2f73ca96a20"
-                    ]
-                ],
-                "6": [
-                    "versions.yml:md5,176f12ceae81f76341e481988c799c15",
-                    "versions.yml:md5,7beadfaf6b22ea0ae6e655b41447803f",
-                    "versions.yml:md5,bfcdd8e2d5151a14dac15a9332d73d52",
-                    "versions.yml:md5,dd8f44a9bfef10555ef1c8cc0267ff9c",
-                    "versions.yml:md5,f2eb7aba102adae159006c9a443c301b"
-                ],
-                "bai": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam.bai:md5,002488588110dcee464e65f68c4726e8"
-                    ]
-                ],
-                "bam": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,2cf8fe8dbba3da7eb4fb251c79f428dc"
-                    ]
-                ],
-                "csi": [
-                    
-                ],
-                "flagstat": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.flagstat:md5,2191911d72575a2358b08b1df64ccb53"
-                    ]
-                ],
-                "idxstats": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.idxstats:md5,613e048487662c694aa4a2f73ca96a20"
-                    ]
-                ],
-                "stats": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.stats:md5,796f45f791f06291b76329528fae0a54"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,176f12ceae81f76341e481988c799c15",
-                    "versions.yml:md5,7beadfaf6b22ea0ae6e655b41447803f",
-                    "versions.yml:md5,bfcdd8e2d5151a14dac15a9332d73d52",
-                    "versions.yml:md5,dd8f44a9bfef10555ef1c8cc0267ff9c",
-                    "versions.yml:md5,f2eb7aba102adae159006c9a443c301b"
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
                 ]
-            }
+            ]
         ],
-        "timestamp": "2023-10-18T09:34:31.989804787"
+        "timestamp": "2023-10-22T20:25:03.687121177"
     },
-    "test_bam_sort_stats_samtools_paired_end": {
+    "test_bam_sort_stats_samtools_paired_end_idxstats": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,81adec7882577c0ad17962599acf7745"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam.bai:md5,9e6427a796975290b1110c9d542ac79d"
-                    ]
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.stats:md5,f3f0e5aad236aae678ac5361b529a664"
-                    ]
-                ],
-                "4": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
-                    ]
-                ],
-                "5": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
-                    ]
-                ],
-                "6": [
-                    "versions.yml:md5,176f12ceae81f76341e481988c799c15",
-                    "versions.yml:md5,7beadfaf6b22ea0ae6e655b41447803f",
-                    "versions.yml:md5,bfcdd8e2d5151a14dac15a9332d73d52",
-                    "versions.yml:md5,dd8f44a9bfef10555ef1c8cc0267ff9c",
-                    "versions.yml:md5,f2eb7aba102adae159006c9a443c301b"
-                ],
-                "bai": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam.bai:md5,9e6427a796975290b1110c9d542ac79d"
-                    ]
-                ],
-                "bam": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.bam:md5,81adec7882577c0ad17962599acf7745"
-                    ]
-                ],
-                "csi": [
-                    
-                ],
-                "flagstat": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
-                    ]
-                ],
-                "idxstats": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
-                    ]
-                ],
-                "stats": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.stats:md5,f3f0e5aad236aae678ac5361b529a664"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,176f12ceae81f76341e481988c799c15",
-                    "versions.yml:md5,7beadfaf6b22ea0ae6e655b41447803f",
-                    "versions.yml:md5,bfcdd8e2d5151a14dac15a9332d73d52",
-                    "versions.yml:md5,dd8f44a9bfef10555ef1c8cc0267ff9c",
-                    "versions.yml:md5,f2eb7aba102adae159006c9a443c301b"
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
                 ]
-            }
+            ]
         ],
-        "timestamp": "2023-10-18T09:34:57.682759147"
+        "timestamp": "2023-10-22T20:25:03.709648916"
+    },
+    "test_bam_sort_stats_samtools_single_end_stats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.stats:md5,f281507081517414eb1a04b2d9c855b2"
+                ]
+            ]
+        ],
+        "timestamp": "2023-12-04T11:06:50.951881479"
+    },
+    "test_bam_sort_stats_samtools_paired_end_stats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.stats:md5,e32e7e49dce1fbe327a89e0fb7bc01b1"
+                ]
+            ]
+        ],
+        "timestamp": "2023-12-04T11:06:59.253905951"
+    },
+    "test_bam_sort_stats_samtools_single_end_idxstats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.idxstats:md5,613e048487662c694aa4a2f73ca96a20"
+                ]
+            ]
+        ],
+        "timestamp": "2023-10-22T20:25:58.451364604"
+    },
+    "test_bam_sort_stats_samtools_single_end_flagstats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.flagstat:md5,2191911d72575a2358b08b1df64ccb53"
+                ]
+            ]
+        ],
+        "timestamp": "2023-10-22T20:25:58.416859285"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/tags.yml b/subworkflows/nf-core/bam_sort_stats_samtools/tests/tags.yml
index a8274109..30b69d6a 100644
--- a/subworkflows/nf-core/bam_sort_stats_samtools/tests/tags.yml
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/tags.yml
@@ -1,2 +1,2 @@
-bam_sort_stats_samtools:
+subworkflows/bam_sort_stats_samtools:
   - subworkflows/nf-core/bam_sort_stats_samtools/**
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
new file mode 100644
index 00000000..97210890
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
@@ -0,0 +1,102 @@
+nextflow_workflow {
+
+    name "Test Workflow BAM_STATS_SAMTOOLS"
+    script "../main.nf"
+    workflow "BAM_STATS_SAMTOOLS"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "bam_stats_samtools"
+    tag "subworkflows/bam_stats_samtools"
+    tag "samtools"
+    tag "samtools/flagstat"
+    tag "samtools/idxstats"
+    tag "samtools/stats"
+
+    test("test_bam_stats_samtools_single_end") {
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            workflow {
+                """
+                    input[0] = [ [ id:'test', single_end:true ], // meta map
+                                file(params.test_data['sarscov2']['illumina']['test_single_end_sorted_bam'], checkIfExists: true),
+                                file(params.test_data['sarscov2']['illumina']['test_single_end_sorted_bam_bai'], checkIfExists: true)
+                        ]
+                    input[1] = [ [ id:'genome' ],
+                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                            ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out.stats).match("test_bam_stats_samtools_single_end_stats") },
+                { assert snapshot(workflow.out.flagstat).match("test_bam_stats_samtools_single_end_flagstats") },
+                { assert snapshot(workflow.out.idxstats).match("test_bam_stats_samtools_single_end_idxstats") }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end") {
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            workflow {
+                """
+                    input[0] = [ [ id:'test', single_end:true ], // meta map
+                                file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
+                                file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
+                        ]
+                    input[1] = [ [ id:'genome' ],
+                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                            ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out.stats).match("test_bam_stats_samtools_paired_end_stats") },
+                { assert snapshot(workflow.out.flagstat).match("test_bam_stats_samtools_paired_end_flagstats") },
+                { assert snapshot(workflow.out.idxstats).match("test_bam_stats_samtools_paired_end_idxstats") }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end_cram") {
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            workflow {
+                """
+                     input[0] = [ [ id:'test', single_end:false ], // meta map
+                                file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true),
+                                file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram_crai'], checkIfExists: true)
+                                ]
+                    input[1] = [ [ id:'genome' ],
+                            file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
+                            ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out.stats).match("test_bam_stats_samtools_paired_end_cram_stats") },
+                { assert snapshot(workflow.out.flagstat).match("test_bam_stats_samtools_paired_end_cram_flagstats") },
+                { assert snapshot(workflow.out.idxstats).match("test_bam_stats_samtools_paired_end_cram_idxstats") }
+            )
+        }
+    }
+
+}
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
new file mode 100644
index 00000000..d3af1376
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
@@ -0,0 +1,128 @@
+{
+    "test_bam_stats_samtools_paired_end_cram_flagstats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.flagstat:md5,a53f3d26e2e9851f7d528442bbfe9781"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-06T09:31:26.194017574"
+    },
+    "test_bam_stats_samtools_paired_end_stats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.stats:md5,49e2b43344ff92bc4c02463a58f7ba4a"
+                ]
+            ]
+        ],
+        "timestamp": "2023-12-04T11:07:13.965061942"
+    },
+    "test_bam_stats_samtools_paired_end_flagstats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-06T09:31:11.668517251"
+    },
+    "test_bam_stats_samtools_single_end_flagstats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.flagstat:md5,2191911d72575a2358b08b1df64ccb53"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-06T09:26:10.340046381"
+    },
+    "test_bam_stats_samtools_paired_end_cram_idxstats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.idxstats:md5,e179601fa7b8ebce81ac3765206f6c15"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-06T09:31:26.207052003"
+    },
+    "test_bam_stats_samtools_single_end_stats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.stats:md5,5a6667d97806e5002731e9cf23674fad"
+                ]
+            ]
+        ],
+        "timestamp": "2023-12-04T11:07:06.676820877"
+    },
+    "test_bam_stats_samtools_paired_end_idxstats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-06T09:31:11.68246157"
+    },
+    "test_bam_stats_samtools_single_end_idxstats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.idxstats:md5,613e048487662c694aa4a2f73ca96a20"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-06T09:26:10.349439801"
+    },
+    "test_bam_stats_samtools_paired_end_cram_stats": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.stats:md5,2cf2fe93596ee3d74f946097b204a629"
+                ]
+            ]
+        ],
+        "timestamp": "2023-12-04T11:07:22.30295557"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/tags.yml b/subworkflows/nf-core/bam_stats_samtools/tests/tags.yml
new file mode 100644
index 00000000..ec2f2d68
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/bam_stats_samtools:
+  - subworkflows/nf-core/bam_stats_samtools/**

From 285cfd0d12a9f02286052ffbe36e933060a1747f Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 11 Jan 2024 13:30:57 +0000
Subject: [PATCH 058/227] Fixing linting errors

---
 assets/multiqc_config.yml                  |  5 +--
 modules/nf-core/multiqc/environment.yml    |  2 +-
 modules/nf-core/multiqc/meta.yml           |  1 -
 modules/nf-core/multiqc/tests/main.nf.test | 48 +++++++++++++++-------
 4 files changed, 37 insertions(+), 19 deletions(-)

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 71a77e0b..11c1b997 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,8 +1,7 @@
 report_comment: >
-
-  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/releases/tag/2.2.3" target="_blank">nf-core/smrnaseq</a>
+  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/tree/dev" target="_blank">nf-core/smrnaseq</a>
   analysis pipeline. For information about how to interpret these results, please see the
-  <a href="https://nf-co.re/smrnaseq/2.2.3/docs/output" target="_blank">documentation</a>.
+  <a href="https://nf-co.re/smrnaseq/dev/docs/output" target="_blank">documentation</a>.
 
 report_section_order:
   "nf-core-smrnaseq-methods-description":
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index bc0bdb5b..7625b752 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::multiqc=1.18
+  - bioconda::multiqc=1.19
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index f1aa660e..45a9bc35 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,4 +1,3 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
 name: multiqc
 description: Aggregate results from bioinformatics analyses across many samples into a single report
 keywords:
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
index c2dad217..d0438eda 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -7,12 +7,9 @@ nextflow_process {
     tag "modules_nfcore"
     tag "multiqc"
 
-    test("MULTIQC: FASTQC") {
+    test("sarscov2 single-end [fastqc]") {
 
         when {
-            params {
-                outdir = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
@@ -26,20 +23,17 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert path(process.out.report.get(0)).exists() },
-                { assert path(process.out.data.get(0)).exists() },
-                { assert path(process.out.versions.get(0)).getText().contains("multiqc") }
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
     }
 
-    test("MULTIQC: FASTQC and a config file") {
+    test("sarscov2 single-end [fastqc] [config]") {
 
         when {
-            params {
-                outdir = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
@@ -53,9 +47,35 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert path(process.out.report.get(0)).exists() },
-                { assert path(process.out.data.get(0)).exists() },
-                { assert path(process.out.versions.get(0)).getText().contains("multiqc") }
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastqc] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.report.collect { file(it).getName() } +
+                                process.out.data.collect { file(it).getName() } +
+                                process.out.plots.collect { file(it).getName() } +
+                                process.out.versions ).match() }
             )
         }
 

From e1b232f42c6db1dc701233ab2f7befe31c62aff7 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 11 Jan 2024 13:54:21 +0000
Subject: [PATCH 059/227] Follow the lead, Timmy

---
 subworkflows/local/umi_dedup.nf               |  8 +-
 .../nf-core/fastqc_umitools_trimgalore.nf     | 78 -------------------
 workflows/smrnaseq.nf                         | 40 ++++------
 3 files changed, 19 insertions(+), 107 deletions(-)
 delete mode 100644 subworkflows/nf-core/fastqc_umitools_trimgalore.nf

diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 12033274..8712f526 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -1,10 +1,10 @@
-// 
+//
 // Deduplicate the UMI reads by mapping them to the complete genome.
 //
 
 include { INDEX_GENOME                        } from '../../modules/local/bowtie_genome'
 include { BOWTIE_MAP_SEQ as UMI_MAP_GENOME    } from '../../modules/local/bowtie_map_mirna'
-include { BAM_SORT_SAMTOOLS                   } from '../../subworkflows/nf-core/bam_sort_samtools'
+include { BAM_SORT_STATS_SAMTOOLS             } from '../../subworkflows/nf-core/bam_sort_stats_samtools'
 include { UMITOOLS_DEDUP                      } from '../../modules/nf-core/modules/umitools/dedup/main'
 include { SAMTOOLS_BAM2FQ                     } from '../../modules/nf-core/modules/samtools/bam2fq/main'
 include { CAT_CAT                             } from '../../modules/nf-core/modules/cat/cat/main'
@@ -31,7 +31,7 @@ workflow DEDUPLICATE_UMIS {
     }
 
     if (bt_index){
-        
+
         UMI_MAP_GENOME ( reads, bt_index.collect() )
         ch_versions = ch_versions.mix(UMI_MAP_GENOME.out.versions)
 
@@ -54,7 +54,7 @@ workflow DEDUPLICATE_UMIS {
                 .join(UMI_MAP_GENOME.out.unmapped)
                 .map { meta, file1, file2 -> [meta, [file1, file2]]}
                 .set { ch_cat }
-    
+
             CAT_CAT ( ch_cat )
             ch_dedup_reads = CAT_CAT.out.file_out
         }
diff --git a/subworkflows/nf-core/fastqc_umitools_trimgalore.nf b/subworkflows/nf-core/fastqc_umitools_trimgalore.nf
deleted file mode 100644
index ca158e7a..00000000
--- a/subworkflows/nf-core/fastqc_umitools_trimgalore.nf
+++ /dev/null
@@ -1,78 +0,0 @@
-//
-// Read QC, UMI extraction and trimming
-//
-
-nextflow.enable.dsl=2
-
-include { FASTQC           } from '../../modules/nf-core/modules/fastqc/main'
-include { UMITOOLS_EXTRACT } from '../../modules/nf-core/modules/umitools/extract/main'
-include { TRIMGALORE       } from '../../modules/nf-core/modules/trimgalore/main'
-
-workflow FASTQC_UMITOOLS_TRIMGALORE {
-    take:
-    reads            // channel: [ val(meta), [ reads ] ]
-    skip_fastqc      // boolean: true/false
-    with_umi         // boolean: true/false
-    skip_trimming    // boolean: true/false
-    umi_discard_read // integer: 0, 1 or 2
-
-    main:
-
-    ch_versions = Channel.empty()
-    fastqc_html = Channel.empty()
-    fastqc_zip  = Channel.empty()
-    if (!skip_fastqc) {
-        FASTQC ( reads ).html.set { fastqc_html }
-        fastqc_zip  = FASTQC.out.zip
-        ch_versions = ch_versions.mix(FASTQC.out.versions.first())
-    }
-
-    umi_reads = reads
-    umi_log   = Channel.empty()
-    if (with_umi) {
-        UMITOOLS_EXTRACT ( reads ).reads.set { umi_reads }
-        umi_log     = UMITOOLS_EXTRACT.out.log
-        ch_versions = ch_versions.mix(UMITOOLS_EXTRACT.out.versions.first())
-
-        // Discard R1 / R2 if required
-        if (umi_discard_read in [1,2]) {
-            UMITOOLS_EXTRACT
-                .out
-                .reads
-                .map { meta, reads ->
-                    if (!meta.single_end) {
-                        meta['single_end'] = true
-                        reads = reads[umi_discard_read % 2]
-                    }
-                    return [ meta, reads ]
-                }
-                .set { umi_reads }
-        }
-    }
-
-    trim_reads = umi_reads
-    trim_html  = Channel.empty()
-    trim_zip   = Channel.empty()
-    trim_log   = Channel.empty()
-    if (!skip_trimming) {
-        TRIMGALORE ( umi_reads ).reads.set { trim_reads }
-        trim_html   = TRIMGALORE.out.html
-        trim_zip    = TRIMGALORE.out.zip
-        trim_log    = TRIMGALORE.out.log
-        ch_versions = ch_versions.mix(TRIMGALORE.out.versions.first())
-    }
-
-    emit:
-    reads = trim_reads // channel: [ val(meta), [ reads ] ]
-
-    fastqc_html        // channel: [ val(meta), [ html ] ]
-    fastqc_zip         // channel: [ val(meta), [ zip ] ]
-
-    umi_log            // channel: [ val(meta), [ log ] ]
-
-    trim_html          // channel: [ val(meta), [ html ] ]
-    trim_zip           // channel: [ val(meta), [ zip ] ]
-    trim_log           // channel: [ val(meta), [ txt ] ]
-
-    versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ]
-}
\ No newline at end of file
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 55f7614b..04529c63 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -64,14 +64,14 @@ if (!params.mirgenedb) {
     if (params.mirgenedb_gff) { mirna_gtf = file(params.mirgenedb_gff, checkIfExists: true) } else { exit 1, "MirGeneDB gff file not found: ${params.mirgenedb_gff}"}
 }
 
-include { INPUT_CHECK        } from '../subworkflows/local/input_check'
-include { FASTQC_UMITOOLS_FASTP } from '../subworkflows/nf-core/fastqc_umitools_trimgalore'
-include { DEDUPLICATE_UMIS           } from '../subworkflows/local/umi_dedup'
-include { CONTAMINANT_FILTER } from '../subworkflows/local/contaminant_filter'
-include { MIRNA_QUANT        } from '../subworkflows/local/mirna_quant'
-include { GENOME_QUANT       } from '../subworkflows/local/genome_quant'
-include { MIRTRACE           } from '../subworkflows/local/mirtrace'
-include { MIRDEEP2           } from '../subworkflows/local/mirdeep2'
+include { INPUT_CHECK           } from '../subworkflows/local/input_check'
+include { FASTQ_FASTQC_UMITOOLS_FASTP } from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp'
+include { DEDUPLICATE_UMIS      } from '../subworkflows/local/umi_dedup'
+include { CONTAMINANT_FILTER    } from '../subworkflows/local/contaminant_filter'
+include { MIRNA_QUANT           } from '../subworkflows/local/mirna_quant'
+include { GENOME_QUANT          } from '../subworkflows/local/genome_quant'
+include { MIRTRACE              } from '../subworkflows/local/mirtrace'
+include { MIRDEEP2              } from '../subworkflows/local/mirdeep2'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -132,22 +132,19 @@ workflow SMRNASEQ {
     ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first().ifEmpty(null))
 
     //
-    // SUBWORKFLOW: Read QC and trim adapters
+    // SUBWORKFLOW: Read QC, extract UMI and trim adapters & dedup UMIs if necessary / desired by the user
     //
 
-    //
-    // SUBWORKFLOW: Read QC, extract UMI and trim adapters
-    //
-    FASTQC_UMITOOLS_FASTP (
+    FASTQ_FASTQC_UMITOOLS_FASTP (
         ch_cat_fastq,
         params.skip_fastqc || params.skip_qc,
         params.with_umi,
         params.skip_trimming,
         params.umi_discard_read
     )
-    ch_versions = ch_versions.mix(FASTQC_UMITOOLS_FASTP.out.versions)
+    ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
 
-    reads_for_mirna = FASTQC_UMITOOLS_FASTP.out.reads
+    reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
 
     //
     // SUBWORKFLOW: Deduplicate UMIs by mapping them to the genome
@@ -156,8 +153,8 @@ workflow SMRNASEQ {
         if (fasta){
             fasta_ch = file(fasta)
             DEDUPLICATE_UMIS (
-                fasta_ch, 
-                bt_index, 
+                fasta_ch,
+                bt_index,
                 FASTQC_UMITOOLS_FASTP.out.reads
             )
             reads_for_mirna = DEDUPLICATE_UMIS.out.reads
@@ -165,18 +162,11 @@ workflow SMRNASEQ {
         }
     }
 
-    FASTQC_UMITOOLS_FASTP (
-        ch_cat_fastq,
-        ch_fastp_adapters,
-        false,
-        false
-    )
-    ch_versions = ch_versions.mix(FASTQC_FASTP.out.versions)
 
     //
     // SUBWORKFLOW: mirtrace QC
     //
-    FASTQC_FASTP.out.adapterseq
+    FASTQ_FASTQC_UMITOOLS_FASTP.out.adapterseq
     .join( FASTQC_FASTP.out.reads )
     .map { meta, adapterseq, reads -> [adapterseq, meta.id, reads] }
     .groupTuple()

From 2828bd2a05dc1d6282dfaa5f4d7a5be96482ae18 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 11 Jan 2024 14:16:18 +0000
Subject: [PATCH 060/227] Improvements to channel handling

---
 subworkflows/local/umi_dedup.nf |  7 ++++---
 workflows/smrnaseq.nf           | 17 ++++++++++++-----
 2 files changed, 16 insertions(+), 8 deletions(-)

diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 8712f526..5a5a393f 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -5,9 +5,10 @@
 include { INDEX_GENOME                        } from '../../modules/local/bowtie_genome'
 include { BOWTIE_MAP_SEQ as UMI_MAP_GENOME    } from '../../modules/local/bowtie_map_mirna'
 include { BAM_SORT_STATS_SAMTOOLS             } from '../../subworkflows/nf-core/bam_sort_stats_samtools'
-include { UMITOOLS_DEDUP                      } from '../../modules/nf-core/modules/umitools/dedup/main'
-include { SAMTOOLS_BAM2FQ                     } from '../../modules/nf-core/modules/samtools/bam2fq/main'
-include { CAT_CAT                             } from '../../modules/nf-core/modules/cat/cat/main'
+include { UMITOOLS_DEDUP                      } from '../../modules/nf-core/umitools/dedup/main'
+include { SAMTOOLS_BAM2FQ                     } from '../../modules/nf-core/samtools/bam2fq/main'
+include { CAT_CAT                             } from '../../modules/nf-core/cat/cat/main'
+
 
 workflow DEDUPLICATE_UMIS {
     take:
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 04529c63..250f1991 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -137,14 +137,21 @@ workflow SMRNASEQ {
 
     FASTQ_FASTQC_UMITOOLS_FASTP (
         ch_cat_fastq,
-        params.skip_fastqc || params.skip_qc,
+        params.skip_fastqc,
         params.with_umi,
+        params.skip_umi_extract,
+        params.umi_discard_read,
         params.skip_trimming,
-        params.umi_discard_read
+        params.umi_discard_read,
+        params.skip_trimming,
+        params.adapter_fasta,
+        params.save_trimmed_fail,
+        params.save_merged,
+        params.min_trimmed_reads
     )
     ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
 
-    reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
+    reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_reads
 
     //
     // SUBWORKFLOW: Deduplicate UMIs by mapping them to the genome
@@ -155,7 +162,7 @@ workflow SMRNASEQ {
             DEDUPLICATE_UMIS (
                 fasta_ch,
                 bt_index,
-                FASTQC_UMITOOLS_FASTP.out.reads
+                FASTQC_UMITOOLS_FASTP.out.trim_reads
             )
             reads_for_mirna = DEDUPLICATE_UMIS.out.reads
             ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)
@@ -167,7 +174,7 @@ workflow SMRNASEQ {
     // SUBWORKFLOW: mirtrace QC
     //
     FASTQ_FASTQC_UMITOOLS_FASTP.out.adapterseq
-    .join( FASTQC_FASTP.out.reads )
+    .join( FASTQ_FASTQC_UMITOOLS_FASTP.out.reads )
     .map { meta, adapterseq, reads -> [adapterseq, meta.id, reads] }
     .groupTuple()
     .set { ch_mirtrace_inputs }

From ff703f10d4bd122210ac0dbe5e1eac55d0e3cf04 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 08:59:12 +0000
Subject: [PATCH 061/227] Update to latest subworkflow version

---
 modules.json                                  |  2 +-
 .../fastq_fastqc_umitools_fastp/main.nf       | 14 +++++++++-
 .../fastq_fastqc_umitools_fastp/meta.yml      | 11 ++++----
 .../tests/main.nf.test                        |  1 +
 .../tests/main.nf.test.snap                   | 28 ++++++++++++++-----
 workflows/smrnaseq.nf                         |  4 +--
 6 files changed, 44 insertions(+), 16 deletions(-)

diff --git a/modules.json b/modules.json
index 56255242..40eda6cc 100644
--- a/modules.json
+++ b/modules.json
@@ -91,7 +91,7 @@
                     },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
-                        "git_sha": "3e8b0c1144ccf60b7848efbdc2be285ff20b49ee",
+                        "git_sha": "668185ddcd2d9084c819691c99020360e0f029a0",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
index 3dbb27ea..49570521 100644
--- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
@@ -17,6 +17,11 @@ def getFastpReadsAfterFiltering(json_file) {
     return json['after_filtering']['total_reads'].toLong()
 }
 
+def getFastpAdapterSequence(json_file){
+    def Map json = (Map) new JsonSlurper().parseText(json_file.text).get('adapter_cutting')
+    return json['read1_adapter_sequence']
+}
+
 workflow FASTQ_FASTQC_UMITOOLS_FASTP {
     take:
     reads             // channel: [ val(meta), [ reads ] ]
@@ -25,7 +30,7 @@ workflow FASTQ_FASTQC_UMITOOLS_FASTP {
     skip_umi_extract  // boolean: true/false
     umi_discard_read  // integer: 0, 1 or 2
     skip_trimming     // boolean: true/false
-    adapter_fasta     //    file: adapter.fasta
+    adapter_fasta     // file: adapter.fasta
     save_trimmed_fail // boolean: true/false
     save_merged       // boolean: true/false
     min_trimmed_reads // integer: > 0
@@ -75,6 +80,8 @@ workflow FASTQ_FASTQC_UMITOOLS_FASTP {
     fastqc_trim_html  = Channel.empty()
     fastqc_trim_zip   = Channel.empty()
     trim_read_count   = Channel.empty()
+    adapter_seq       = Channel.empty()
+
     if (!skip_trimming) {
         FASTP (
             umi_reads,
@@ -108,6 +115,10 @@ workflow FASTQ_FASTQC_UMITOOLS_FASTP {
             .map { meta, reads, num_reads -> [ meta, num_reads ] }
             .set { trim_read_count }
 
+        trim_json
+            .map { meta, json -> [meta, getFastpAdapterSequence(json)] }
+            .set { adapter_seq }
+
         if (!skip_fastqc) {
             FASTQC_TRIM (
                 trim_reads
@@ -125,6 +136,7 @@ workflow FASTQ_FASTQC_UMITOOLS_FASTP {
     fastqc_raw_zip     // channel: [ val(meta), [ zip ] ]
 
     umi_log            // channel: [ val(meta), [ log ] ]
+    adapter_seq        // channel: [ val(meta), [ adapter_seq] ]
 
     trim_json          // channel: [ val(meta), [ json ] ]
     trim_html          // channel: [ val(meta), [ html ] ]
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml
index 220e8db1..9308fe9b 100644
--- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml
@@ -43,7 +43,7 @@ input:
   - skip_trimming:
       type: boolean
       description: |
-        Allows to skip trimgalore execution
+        Allows to skip FastP execution
   - adapter_fasta:
       type: file
       description: |
@@ -70,10 +70,7 @@ output:
       type: file
       description: >
         Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. |
-
-
-
-          For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
+        For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
       pattern: "*.{fastq.gz}"
   - fastqc_html:
       type: file
@@ -118,6 +115,10 @@ output:
       type: file
       description: FastQC report archive
       pattern: "*_{fastqc.zip}"
+  - adapter_seq:
+      type: string
+      description: |
+        Adapter Sequence found in read1
   - versions:
       type: file
       description: File containing software versions
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
index cdd73984..91dec8c5 100644
--- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
@@ -45,6 +45,7 @@ nextflow_workflow {
                 { assert snapshot(workflow.out.trim_json).match("trim_json") },
                 { assert snapshot(workflow.out.trim_reads_fail).match("trim_reads_fail") },
                 { assert snapshot(workflow.out.trim_reads_merged).match("trim_reads_merged") },
+                { assert snapshot(workflow.out.adapter_seq).match("adapter_seq") },
                 { assert snapshot(workflow.out.trim_read_count).match("trim_read_count") },
                 { assert snapshot(workflow.out.versions).match("versions") },
 
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
index 38a65aeb..973746a3 100644
--- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
@@ -5,7 +5,7 @@
                 
             ]
         ],
-        "timestamp": "2023-11-26T02:28:26.26920982"
+        "timestamp": "2024-01-12T08:38:50.041635573"
     },
     "trim_reads_fail": {
         "content": [
@@ -13,7 +13,7 @@
                 
             ]
         ],
-        "timestamp": "2023-11-26T02:28:26.25861515"
+        "timestamp": "2024-01-12T08:38:50.033284693"
     },
     "versions": {
         "content": [
@@ -23,7 +23,7 @@
                 "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
             ]
         ],
-        "timestamp": "2023-11-26T02:28:26.30891403"
+        "timestamp": "2024-01-12T08:38:50.121510557"
     },
     "trim_json": {
         "content": [
@@ -37,7 +37,21 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-26T02:28:26.24768259"
+        "timestamp": "2024-01-12T08:38:50.024410724"
+    },
+    "adapter_seq": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "unspecified"
+                ]
+            ]
+        ],
+        "timestamp": "2024-01-12T08:38:50.08674429"
     },
     "reads": {
         "content": [
@@ -54,7 +68,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-12-04T11:30:32.061644815"
+        "timestamp": "2024-01-12T08:38:49.994419936"
     },
     "umi_log": {
         "content": [
@@ -62,7 +76,7 @@
                 
             ]
         ],
-        "timestamp": "2023-11-26T02:28:26.238536"
+        "timestamp": "2024-01-12T08:38:50.017720214"
     },
     "trim_read_count": {
         "content": [
@@ -76,6 +90,6 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-26T02:28:26.27984169"
+        "timestamp": "2024-01-12T08:38:50.102326089"
     }
 }
\ No newline at end of file
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 250f1991..5ea0df5e 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -173,9 +173,9 @@ workflow SMRNASEQ {
     //
     // SUBWORKFLOW: mirtrace QC
     //
-    FASTQ_FASTQC_UMITOOLS_FASTP.out.adapterseq
+    FASTQ_FASTQC_UMITOOLS_FASTP.out.adapter_seq
     .join( FASTQ_FASTQC_UMITOOLS_FASTP.out.reads )
-    .map { meta, adapterseq, reads -> [adapterseq, meta.id, reads] }
+    .map { meta, adapter_seq, reads -> [adapter_seq, meta.id, reads] }
     .groupTuple()
     .set { ch_mirtrace_inputs }
 

From 313e7d3f899b4e400912480f50c20c40b4bd7d34 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 09:19:36 +0000
Subject: [PATCH 062/227] Cleaning up

---
 nextflow.config       |  1 +
 nextflow_schema.json  | 11 ++---------
 workflows/smrnaseq.nf |  6 ++----
 3 files changed, 5 insertions(+), 13 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index a223bc26..886011cd 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -47,6 +47,7 @@ params {
     fastp_min_length            = 17
     fastp_known_mirna_adapters  = "$projectDir/assets/known_adapters.fa"
     save_trimmed_fail           = false
+    save_merged                 = false
     skip_fastqc                 = false
     skip_multiqc                = false
     skip_mirdeep                = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 33b150b4..d736635e 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -92,7 +92,8 @@
                 "umi_merge_unmapped": {
                     "type": "boolean",
                     "fa_icon": "fas fa-save",
-                    "description": "Unless deactivated the deduplicated reads are merged with the reads that could not be placed to reduce the potential reference bias"
+                    "description": "Unless deactivated the deduplicated reads are merged with the reads that could not be placed to reduce the potential reference bias",
+                    "default": true
                 }
             },
             "fa_icon": "fas fa-barcode"
@@ -209,7 +210,6 @@
                 },
                 "three_prime_adapter": {
                     "type": "string",
-                    "default": "TGGAATTCTCGGGTGCCAAGG",
                     "fa_icon": "fas fa-text-width",
                     "description": "Sequencing adapter sequence to use for trimming."
                 },
@@ -408,14 +408,12 @@
                     "type": "boolean",
                     "description": "Display help text.",
                     "fa_icon": "fas fa-question-circle",
-                    "default": false,
                     "hidden": true
                 },
                 "version": {
                     "type": "boolean",
                     "description": "Display version and exit.",
                     "fa_icon": "fas fa-question-circle",
-                    "default": false,
                     "hidden": true
                 },
                 "publish_dir_mode": {
@@ -439,7 +437,6 @@
                     "type": "boolean",
                     "description": "Send plain-text email instead of HTML.",
                     "fa_icon": "fas fa-remove-format",
-                    "default": false,
                     "hidden": true
                 },
                 "max_multiqc_email_size": {
@@ -454,7 +451,6 @@
                     "type": "boolean",
                     "description": "Do not use coloured log outputs.",
                     "fa_icon": "fas fa-palette",
-                    "default": false,
                     "hidden": true
                 },
                 "hook_url": {
@@ -493,7 +489,6 @@
                     "type": "boolean",
                     "fa_icon": "far fa-eye-slash",
                     "description": "Show all params when using `--help`",
-                    "default": false,
                     "hidden": true,
                     "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters."
                 },
@@ -501,7 +496,6 @@
                     "type": "boolean",
                     "fa_icon": "far fa-check-circle",
                     "description": "Validation of parameters fails when an unrecognised parameter is found.",
-                    "default": false,
                     "hidden": true,
                     "help_text": "By default, when an unrecognised parameter is found, it returns a warinig."
                 },
@@ -509,7 +503,6 @@
                     "type": "boolean",
                     "fa_icon": "far fa-check-circle",
                     "description": "Validation of parameters in lenient more.",
-                    "default": false,
                     "hidden": true,
                     "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)."
                 }
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 5ea0df5e..11fb44e5 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -142,16 +142,14 @@ workflow SMRNASEQ {
         params.skip_umi_extract,
         params.umi_discard_read,
         params.skip_trimming,
-        params.umi_discard_read,
-        params.skip_trimming,
-        params.adapter_fasta,
+        params.fastp_known_mirna_adapters,
         params.save_trimmed_fail,
         params.save_merged,
         params.min_trimmed_reads
     )
     ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
 
-    reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_reads
+    reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
 
     //
     // SUBWORKFLOW: Deduplicate UMIs by mapping them to the genome

From 5e3f0824f619aa7d94b2e2a25a6b190e6265b32b Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 09:30:47 +0000
Subject: [PATCH 063/227] Some more fixes / cleanup

---
 nextflow.config       |  3 ++-
 nextflow_schema.json  |  3 +++
 workflows/smrnaseq.nf | 12 ++++++------
 3 files changed, 11 insertions(+), 7 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 886011cd..e476d297 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -54,7 +54,8 @@ params {
     skip_fastp                  = false
     save_reference              = false
     fastp_max_length            = 40
-
+    min_trimmed_reads           = 10
+    
     // Contamination filtering
     filter_contamination        = false
     rrna                        = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index d736635e..a846609e 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -505,6 +505,9 @@
                     "description": "Validation of parameters in lenient more.",
                     "hidden": true,
                     "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)."
+                },
+                "save_merged": {
+                    "type": "boolean"
                 }
             }
         }
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 11fb44e5..bf9c3ac8 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -141,7 +141,7 @@ workflow SMRNASEQ {
         params.with_umi,
         params.skip_umi_extract,
         params.umi_discard_read,
-        params.skip_trimming,
+        params.skip_fastp,
         params.fastp_known_mirna_adapters,
         params.save_trimmed_fail,
         params.save_merged,
@@ -185,7 +185,7 @@ workflow SMRNASEQ {
     // SUBWORKFLOW: remove contaminants from reads
     //
     contamination_stats = Channel.empty()
-    mirna_reads = FASTQC_FASTP.out.reads
+    mirna_reads = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
     if (params.filter_contamination){
         CONTAMINANT_FILTER (
             reference_hairpin,
@@ -195,7 +195,7 @@ workflow SMRNASEQ {
             params.ncrna,
             params.pirna,
             params.other_contamination,
-            FASTQC_FASTP.out.reads
+            FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
         )
 
         contamination_stats = CONTAMINANT_FILTER.out.filter_stats
@@ -223,7 +223,7 @@ workflow SMRNASEQ {
 
         if (!params.skip_mirdeep) {
             MIRDEEP2 (
-                FASTQC_UMITOOLS_FASTP.out.reads,
+                FASTQ_FASTQC_UMITOOLS_FASTP.out.reads,
                 GENOME_QUANT.out.fasta,
                 GENOME_QUANT.out.index.collect(),
                 MIRNA_QUANT.out.fasta_hairpin,
@@ -252,8 +252,8 @@ workflow SMRNASEQ {
         ch_multiqc_files = Channel.empty()
         ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
         ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-        ch_multiqc_files = ch_multiqc_files.mix(FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files = ch_multiqc_files.mix(FASTQC_FASTP.out.trim_json.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_json.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(contamination_stats.collect().ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(genome_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mature_stats.collect({it[1]}).ifEmpty([]))

From 6240ec0ac8d10372491a3400b6881540a62674f6 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 09:31:48 +0000
Subject: [PATCH 064/227] Clean

---
 nextflow.config | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index e476d297..ef869a71 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -55,7 +55,7 @@ params {
     save_reference              = false
     fastp_max_length            = 40
     min_trimmed_reads           = 10
-    
+
     // Contamination filtering
     filter_contamination        = false
     rrna                        = null
@@ -65,7 +65,6 @@ params {
     pirna                       = null
     other_contamination         = null
 
-
     // MultiQC options
     multiqc_config              = null
     multiqc_title               = null

From bfcf4863db204e94c604bf0c4b0ac0285105ec76 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 09:55:58 +0000
Subject: [PATCH 065/227] Improved error handling

---
 subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf | 9 +++++++--
 1 file changed, 7 insertions(+), 2 deletions(-)

diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
index 49570521..764ce013 100644
--- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
@@ -18,8 +18,13 @@ def getFastpReadsAfterFiltering(json_file) {
 }
 
 def getFastpAdapterSequence(json_file){
-    def Map json = (Map) new JsonSlurper().parseText(json_file.text).get('adapter_cutting')
-    return json['read1_adapter_sequence']
+    def Map json = (Map) new JsonSlurper().parseText(json_file.text)
+    try{
+        adapter = json['adapter_cutting']['read1_adapter_sequence']
+    } catch(Exception ex){
+        adapter = ""
+    }
+    return adapter
 }
 
 workflow FASTQ_FASTQC_UMITOOLS_FASTP {

From d5d1bfcd31c209a8ea39b5fe3901d726c06e9d0d Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <apeltzer@users.noreply.github.com>
Date: Fri, 12 Jan 2024 11:07:48 +0100
Subject: [PATCH 066/227] Update nextflow_schema.json

---
 nextflow_schema.json | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index a846609e..b1106dcd 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -241,6 +241,12 @@
                     "default": "${projectDir}/assets/known_adapters.fa",
                     "description": "FastA with known miRNA adapter sequences for adapter trimming",
                     "fa_icon": "far fa-question-circle"
+                },
+                "min_trimmed_reads": {
+                    "type": "integer",
+                    "default": 10,
+                    "fa_icon": "far fa-window-minimize",
+                    "description": "Minimum number of reads required in input file to use it"
                 }
             }
         },

From 22f07dead81b26b3664c054b88e43b5e4610b2fe Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 10:48:46 +0000
Subject: [PATCH 067/227] Fix for UMI fasta

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 9caf0d5e..314c8d75 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -155,7 +155,7 @@ workflow SMRNASEQ {
     // SUBWORKFLOW: Deduplicate UMIs by mapping them to the genome
     //
     if (params.with_umi){
-        if (fasta){
+        if (params.fasta){
             fasta_ch = file(fasta)
             DEDUPLICATE_UMIS (
                 fasta_ch,

From 8fb0ae060ca6596d9245fcbff6df4db2bf17513a Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 10:54:45 +0000
Subject: [PATCH 068/227] Fix params.fasta

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 314c8d75..ee62cb26 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -156,7 +156,7 @@ workflow SMRNASEQ {
     //
     if (params.with_umi){
         if (params.fasta){
-            fasta_ch = file(fasta)
+            fasta_ch = file(params.fasta)
             DEDUPLICATE_UMIS (
                 fasta_ch,
                 bt_index,

From 227af283d143ca3325344ed4c76abc674ab5e5fe Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 11:09:07 +0000
Subject: [PATCH 069/227] Minor updates

---
 modules/local/edger_qc.nf         | 2 +-
 modules/local/mirdeep2_prepare.nf | 6 +++---
 workflows/smrnaseq.nf             | 9 ++++++++-
 3 files changed, 12 insertions(+), 5 deletions(-)

diff --git a/modules/local/edger_qc.nf b/modules/local/edger_qc.nf
index 729d5eed..5560de23 100644
--- a/modules/local/edger_qc.nf
+++ b/modules/local/edger_qc.nf
@@ -1,7 +1,7 @@
 process EDGER_QC {
     label 'process_medium'
 
-    conda 'bioconda::bioconductor-limma=3.50.0 bioconda::bioconductor-edger=3.36.0 conda-forge::r-data.table=1.14.2 conda-forge::r-gplots=3.1.1 conda-forge::r-statmod=1.4.36'
+    conda 'bioconda::bioconductor-limma=3.58.1 bioconda::bioconductor-edger=4.0.2 conda-forge::r-data.table=1.14.10 conda-forge::r-gplots=3.1.3 conda-forge::r-statmod=1.5.0'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/mulled-v2-419bd7f10b2b902489ac63bbaafc7db76f8e0ae1:709335c37934db1b481054cbec637c6e5b5971cb-0' :
         'biocontainers/mulled-v2-419bd7f10b2b902489ac63bbaafc7db76f8e0ae1:709335c37934db1b481054cbec637c6e5b5971cb-0' }"
diff --git a/modules/local/mirdeep2_prepare.nf b/modules/local/mirdeep2_prepare.nf
index 124b5b63..ce66b9f1 100644
--- a/modules/local/mirdeep2_prepare.nf
+++ b/modules/local/mirdeep2_prepare.nf
@@ -3,10 +3,10 @@ process MIRDEEP2_PIGZ {
     tag "$meta.id"
 
     // TODO maybe create a mulled container and uncompress within mirdeep2_mapper?
-    conda 'bioconda::bioconvert=0.4.3'
+    conda 'bioconda::bioconvert=1.1.1'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bioconvert:0.4.3--py_0' :
-        'biocontainers/bioconvert:0.4.3--py_0' }"
+        'https://depot.galaxyproject.org/singularity/bioconvert:1.1.1--pyhdfd78af_0' :
+        'biocontainers/bioconvert:1.1.1--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(reads)
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index ee62cb26..95e5b810 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -72,6 +72,7 @@ include { MIRNA_QUANT           } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT          } from '../subworkflows/local/genome_quant'
 include { MIRTRACE              } from '../subworkflows/local/mirtrace'
 include { MIRDEEP2              } from '../subworkflows/local/mirdeep2'
+include { INDEX_GENOME          } from '../../modules/local/bowtie_genome'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -157,9 +158,15 @@ workflow SMRNASEQ {
     if (params.with_umi){
         if (params.fasta){
             fasta_ch = file(params.fasta)
+
+            //This needs to be done here as GENOME_QUANT should not run prior to the deduplication of UMIs.
+            INDEX_GENOME ( fasta )
+
+            ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)
+
             DEDUPLICATE_UMIS (
                 fasta_ch,
-                bt_index,
+                INDEX_GENOME.out.bowtie_indices,
                 FASTQC_UMITOOLS_FASTP.out.trim_reads
             )
             reads_for_mirna = DEDUPLICATE_UMIS.out.reads

From eed08955d5fd4c018d216d772e653df0f209307a Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 11:11:05 +0000
Subject: [PATCH 070/227] Fixing some lint warnings

---
 modules/local/bowtie_contaminants.nf     | 2 +-
 modules/local/bowtie_genome.nf           | 2 +-
 modules/local/bowtie_map_contaminants.nf | 2 +-
 modules/local/bowtie_mirna.nf            | 2 +-
 modules/local/filter_stats.nf            | 2 +-
 modules/local/seqcluster_collapse.nf     | 2 +-
 6 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/modules/local/bowtie_contaminants.nf b/modules/local/bowtie_contaminants.nf
index e6a594a7..cf02de31 100644
--- a/modules/local/bowtie_contaminants.nf
+++ b/modules/local/bowtie_contaminants.nf
@@ -4,7 +4,7 @@ process INDEX_CONTAMINANTS {
     conda 'bowtie2=2.4.5'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/bowtie2:2.4.5--py39hd2f7db1_2' :
-        'biocontainers/bowtie2:2.4.5--py36hfca12d5_2'}"
+        'biocontainers/bowtie2:2.4.5--py39hd2f7db1_2'}"
 
     input:
     path fasta
diff --git a/modules/local/bowtie_genome.nf b/modules/local/bowtie_genome.nf
index 91a6cd53..4e1ea7ca 100644
--- a/modules/local/bowtie_genome.nf
+++ b/modules/local/bowtie_genome.nf
@@ -4,7 +4,7 @@ process INDEX_GENOME {
 
     conda 'bioconda::bowtie=1.3.1-4'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bowtie%3A1.3.1--py39hd400a0c_2' :
+        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1--py310h4070885_4' :
         'biocontainers/bowtie:1.3.1--py310h4070885_4' }"
 
     input:
diff --git a/modules/local/bowtie_map_contaminants.nf b/modules/local/bowtie_map_contaminants.nf
index d744b1fd..c9863ab3 100644
--- a/modules/local/bowtie_map_contaminants.nf
+++ b/modules/local/bowtie_map_contaminants.nf
@@ -4,7 +4,7 @@ process BOWTIE_MAP_CONTAMINANTS {
     conda 'bowtie2=2.4.5'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/bowtie2:2.4.5--py39hd2f7db1_2' :
-        'biocontainers/bowtie2:2.4.5--py36hfca12d5_2' }"
+        'biocontainers/bowtie2:2.4.5--py39hd2f7db1_2' }"
 
     input:
     tuple val(meta), path(reads)
diff --git a/modules/local/bowtie_mirna.nf b/modules/local/bowtie_mirna.nf
index 2be45bb8..cb7f0d0f 100644
--- a/modules/local/bowtie_mirna.nf
+++ b/modules/local/bowtie_mirna.nf
@@ -3,7 +3,7 @@ process INDEX_MIRNA {
 
     conda 'bioconda::bowtie=1.3.0-2'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bowtie%3A1.3.1--py39hd400a0c_2' :
+        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1--py310h4070885_4' :
         'biocontainers/bowtie:1.3.1--py310h4070885_4' }"
 
     input:
diff --git a/modules/local/filter_stats.nf b/modules/local/filter_stats.nf
index 18e7016b..1e5494cd 100644
--- a/modules/local/filter_stats.nf
+++ b/modules/local/filter_stats.nf
@@ -4,7 +4,7 @@ process FILTER_STATS {
     conda 'bowtie2=2.4.5'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/bowtie2:2.4.5--py39hd2f7db1_2' :
-        'biocontainers/bowtie2:2.4.5--py36hfca12d5_2' }"
+        'biocontainers/bowtie2:2.4.5--py39hd2f7db1_2' }"
 
     input:
     tuple val(meta), path(reads)
diff --git a/modules/local/seqcluster_collapse.nf b/modules/local/seqcluster_collapse.nf
index 82470e5a..826f8cef 100644
--- a/modules/local/seqcluster_collapse.nf
+++ b/modules/local/seqcluster_collapse.nf
@@ -5,7 +5,7 @@ process SEQCLUSTER_SEQUENCES {
     conda 'bioconda::seqcluster=1.2.9-0'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/seqcluster:1.2.9--pyh5e36f6f_0' :
-        'biocontainers/seqcluster:1.2.8--pyh5e36f6f_0' }"
+        'biocontainers/seqcluster:1.2.9--pyh5e36f6f_0' }"
 
     input:
     tuple val(meta), path(reads)

From 2cc2a0df4df8462b0ba6c658801afb19988bd898 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 11:18:23 +0000
Subject: [PATCH 071/227] Adding in versions for filter stats

---
 modules/local/filter_stats.nf            |   8 ++
 subworkflows/local/contaminant_filter.nf |   4 +-
 subworkflows/local/fastqc_fastp.nf       | 116 -----------------------
 3 files changed, 10 insertions(+), 118 deletions(-)
 delete mode 100644 subworkflows/local/fastqc_fastp.nf

diff --git a/modules/local/filter_stats.nf b/modules/local/filter_stats.nf
index 1e5494cd..4c46f51d 100644
--- a/modules/local/filter_stats.nf
+++ b/modules/local/filter_stats.nf
@@ -13,6 +13,7 @@ process FILTER_STATS {
     output:
     path "*_mqc.yaml"                           , emit: stats
     tuple val(meta), path('*.filtered.fastq.gz'), emit: reads
+    path "versions.yml"                         , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,5 +25,12 @@ process FILTER_STATS {
     tr '\n' ', ' | \\
     awk -v sample=${meta.id} -v readnumber=\$readnumber '{ print "id: \\"my_pca_section\\"\\nsection_name: \\"Contamination Filtering\\"\\ndescription: \\"This plot shows the amount of reads filtered by contaminant type.\\"\\nplot_type: \\"bargraph\\"\\npconfig:\\n  id: \\"contamination_filter_plot\\"\\n  title: \\"Contamination Plot\\"\\n  ylab: \\"Number of reads\\"\\ndata:\\n    "sample": {"\$0"\\"remaining reads\\": "readnumber"}" }' > ${meta.id}.contamination_mqc.yaml
     gzip -c ${reads} > ${meta.id}.filtered.fastq.gz
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        cat:  \$(cat --version | grep 'cat ' |sed 's/cat (GNU coreutils) //')
+        gzip: \$(gzip --version | grep "gzip" | sed 's/gzip //')
+        tr:  \$(tr --version | grep 'tr ' |sed 's/tr (GNU coreutils) //')
+    END_VERSIONS
     """
 }
diff --git a/subworkflows/local/contaminant_filter.nf b/subworkflows/local/contaminant_filter.nf
index 383c85ad..dede115d 100644
--- a/subworkflows/local/contaminant_filter.nf
+++ b/subworkflows/local/contaminant_filter.nf
@@ -123,6 +123,6 @@ workflow CONTAMINANT_FILTER {
 
     emit:
     filtered_reads = FILTER_STATS.out.reads
-    versions = ch_versions
+    versions = ch_versions.mix(FILTER_STATS.out.versions)
     filter_stats = FILTER_STATS.out.stats
-}
\ No newline at end of file
+}
diff --git a/subworkflows/local/fastqc_fastp.nf b/subworkflows/local/fastqc_fastp.nf
deleted file mode 100644
index 9e4d952e..00000000
--- a/subworkflows/local/fastqc_fastp.nf
+++ /dev/null
@@ -1,116 +0,0 @@
-//
-// Read QC and trimming
-//
-
-include { FASTQC as FASTQC_RAW  } from '../../modules/nf-core/fastqc/main'
-include { FASTQC as FASTQC_TRIM } from '../../modules/nf-core/fastqc/main'
-include { FASTP                 } from '../../modules/nf-core/fastp/main'
-
-//
-// Function that parses fastp json output file to get total number of reads after trimming
-//
-import groovy.json.JsonSlurper
-
-def getFastpReadsAfterFiltering(json_file) {
-    return new JsonSlurper().parseText(json_file.text)
-    ?.get('summary')
-    ?.get('after_filtering')
-    ?.get('total_reads')
-    ?.toInteger()
-}
-
-String getFastpAdapterSequence(json_file){
-    return new JsonSlurper().parseText(json_file.text)
-    ?.get('adapter_cutting')
-    ?.get('read1_adapter_sequence')
-}
-
-workflow FASTQC_FASTP {
-    take:
-    reads             // channel: [ val(meta), [ reads ] ]
-    adapter_list      // channel: [ path/to/adapters.fa ]
-    save_trimmed_fail //   value: boolean
-    save_merged       //   value: boolean
-
-
-    main:
-
-    ch_versions     = Channel.empty()
-    fastqc_raw_html = Channel.empty()
-    fastqc_raw_zip  = Channel.empty()
-    adapterseq      = reads.map { meta, _ -> [meta, null] }
-    if (!params.skip_fastqc) {
-        FASTQC_RAW (
-            reads
-        )
-        fastqc_raw_html = FASTQC_RAW.out.html
-        fastqc_raw_zip  = FASTQC_RAW.out.zip
-        ch_versions     = ch_versions.mix(FASTQC_RAW.out.versions.first())
-    }
-
-    trim_reads        = reads
-    trim_json         = Channel.empty()
-    trim_html         = Channel.empty()
-    trim_log          = Channel.empty()
-    trim_reads_fail   = Channel.empty()
-    trim_reads_merged = Channel.empty()
-    fastqc_trim_html  = Channel.empty()
-    fastqc_trim_zip   = Channel.empty()
-    if (!params.skip_fastp) {
-        FASTP (
-            reads,
-            adapter_list,
-            save_trimmed_fail,
-            save_merged
-        )
-        trim_reads        = FASTP.out.reads
-        trim_json         = FASTP.out.json
-        trim_html         = FASTP.out.html
-        trim_log          = FASTP.out.log
-        trim_reads_fail   = FASTP.out.reads_fail
-        trim_reads_merged = FASTP.out.reads_merged
-        ch_versions       = ch_versions.mix(FASTP.out.versions.first())
-
-        //
-        // Filter empty FastQ files after adapter trimming so FastQC doesn't fail
-        //
-        trim_reads
-            .join(trim_json)
-            .map {
-                meta, reads, json ->
-                    if (getFastpReadsAfterFiltering(json) > 0) {
-                        [ meta, reads ]
-                    }
-            }
-            .set { trim_reads }
-
-        trim_json
-            .map { meta, json -> [meta, getFastpAdapterSequence(json)] }
-            .set { adapterseq }
-
-        if (!params.skip_fastqc) {
-            FASTQC_TRIM (
-                trim_reads
-            )
-            fastqc_trim_html = FASTQC_TRIM.out.html
-            fastqc_trim_zip  = FASTQC_TRIM.out.zip
-            ch_versions      = ch_versions.mix(FASTQC_TRIM.out.versions.first())
-        }
-    }
-
-    emit:
-    reads = trim_reads // channel: [ val(meta), [ reads ] ]
-    trim_json          // channel: [ val(meta), [ json ] ]
-    trim_html          // channel: [ val(meta), [ html ] ]
-    trim_log           // channel: [ val(meta), [ log ] ]
-    trim_reads_fail    // channel: [ val(meta), [ fastq.gz ] ]
-    trim_reads_merged  // channel: [ val(meta), [ fastq.gz ] ]
-    adapterseq         // channel: [ val(meta), [ adapterseq ] ]
-
-    fastqc_raw_html    // channel: [ val(meta), [ html ] ]
-    fastqc_raw_zip     // channel: [ val(meta), [ zip ] ]
-    fastqc_trim_html   // channel: [ val(meta), [ html ] ]
-    fastqc_trim_zip    // channel: [ val(meta), [ zip ] ]
-
-    versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ]
-}

From bf6e8bde711df521183e67a7ed3ef6364185bacb Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 11:21:49 +0000
Subject: [PATCH 072/227] Fixing more conda imports

---
 modules/local/bowtie_genome.nf | 6 +++---
 modules/local/bowtie_mirna.nf  | 6 +++---
 2 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/modules/local/bowtie_genome.nf b/modules/local/bowtie_genome.nf
index 4e1ea7ca..5ccb0afa 100644
--- a/modules/local/bowtie_genome.nf
+++ b/modules/local/bowtie_genome.nf
@@ -2,10 +2,10 @@ process INDEX_GENOME {
     tag "$fasta"
     label 'process_medium'
 
-    conda 'bioconda::bowtie=1.3.1-4'
+    conda 'bioconda::bowtie=1.3.1-6'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1--py310h4070885_4' :
-        'biocontainers/bowtie:1.3.1--py310h4070885_4' }"
+        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1-py310h30d4ff4_6' :
+        'biocontainers/bowtie:1.3.1-py310h30d4ff4_6' }"
 
     input:
     tuple val(meta2), path(fasta)
diff --git a/modules/local/bowtie_mirna.nf b/modules/local/bowtie_mirna.nf
index cb7f0d0f..08521095 100644
--- a/modules/local/bowtie_mirna.nf
+++ b/modules/local/bowtie_mirna.nf
@@ -1,10 +1,10 @@
 process INDEX_MIRNA {
     label 'process_medium'
 
-    conda 'bioconda::bowtie=1.3.0-2'
+    conda 'bioconda::bowtie=1.3.1-6'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1--py310h4070885_4' :
-        'biocontainers/bowtie:1.3.1--py310h4070885_4' }"
+        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1-py310h30d4ff4_6' :
+        'biocontainers/bowtie:1.3.1-py310h30d4ff4_6' }"
 
     input:
     tuple val(meta2), path(fasta)

From 454504d1b415bb2b88dd5d7bda25d5599d3e0145 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 11:28:40 +0000
Subject: [PATCH 073/227] More updates of modules

---
 modules/local/bowtie_genome.nf       | 4 ++--
 modules/local/bowtie_mirna.nf        | 4 ++--
 modules/local/format_fasta_mirna.nf  | 6 +++---
 modules/local/mirdeep2_mapper.nf     | 2 +-
 modules/local/mirdeep2_run.nf        | 2 +-
 modules/local/parse_fasta_mirna.nf   | 6 +++---
 modules/local/seqcluster_collapse.nf | 2 +-
 7 files changed, 13 insertions(+), 13 deletions(-)

diff --git a/modules/local/bowtie_genome.nf b/modules/local/bowtie_genome.nf
index 5ccb0afa..60f33f1a 100644
--- a/modules/local/bowtie_genome.nf
+++ b/modules/local/bowtie_genome.nf
@@ -2,9 +2,9 @@ process INDEX_GENOME {
     tag "$fasta"
     label 'process_medium'
 
-    conda 'bioconda::bowtie=1.3.1-6'
+    conda 'bioconda::bowtie=1.3.1'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1-py310h30d4ff4_6' :
+        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1--py310h7b97f60_6' :
         'biocontainers/bowtie:1.3.1-py310h30d4ff4_6' }"
 
     input:
diff --git a/modules/local/bowtie_mirna.nf b/modules/local/bowtie_mirna.nf
index 08521095..2fbf1588 100644
--- a/modules/local/bowtie_mirna.nf
+++ b/modules/local/bowtie_mirna.nf
@@ -1,9 +1,9 @@
 process INDEX_MIRNA {
     label 'process_medium'
 
-    conda 'bioconda::bowtie=1.3.1-6'
+    conda 'bioconda::bowtie=1.3.1'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1-py310h30d4ff4_6' :
+        'https://depot.galaxyproject.org/singularity/bowtie:1.3.1--py310h7b97f60_6' :
         'biocontainers/bowtie:1.3.1-py310h30d4ff4_6' }"
 
     input:
diff --git a/modules/local/format_fasta_mirna.nf b/modules/local/format_fasta_mirna.nf
index 489879a5..67461d64 100644
--- a/modules/local/format_fasta_mirna.nf
+++ b/modules/local/format_fasta_mirna.nf
@@ -4,10 +4,10 @@ process FORMAT_FASTA_MIRNA {
     tag "$fasta"
     label 'process_medium'
 
-    conda 'bioconda::fastx_toolkit=0.0.14-9'
+    conda 'bioconda::fastx_toolkit=0.0.14'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/fastx_toolkit:0.0.14--he1b5a44_8' :
-        'biocontainers/fastx_toolkit:0.0.14--he1b5a44_8' }"
+        'https://depot.galaxyproject.org/singularity/fastx_toolkit:0.0.14--hdbdd923_11' :
+        'biocontainers/fastx_toolkit:0.0.14--hdbdd923_11' }"
 
     input:
     tuple val(meta2), path(fasta)
diff --git a/modules/local/mirdeep2_mapper.nf b/modules/local/mirdeep2_mapper.nf
index 842af6e6..19a9c5dc 100644
--- a/modules/local/mirdeep2_mapper.nf
+++ b/modules/local/mirdeep2_mapper.nf
@@ -4,7 +4,7 @@ process MIRDEEP2_MAPPER {
     label 'process_medium'
     tag "$meta.id"
 
-    conda 'bioconda::mirdeep2=2.0.1'
+    conda 'bioconda::mirdeep2=2.0.1.3'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/mirdeep2:2.0.1.3--hdfd78af_1' :
         'biocontainers/mirdeep2:2.0.1.3--hdfd78af_1' }"
diff --git a/modules/local/mirdeep2_run.nf b/modules/local/mirdeep2_run.nf
index 9b18ed7d..442f26f3 100644
--- a/modules/local/mirdeep2_run.nf
+++ b/modules/local/mirdeep2_run.nf
@@ -4,7 +4,7 @@ process MIRDEEP2_RUN {
     label 'process_medium'
     errorStrategy 'ignore'
 
-    conda 'bioconda::mirdeep2=2.0.1'
+    conda 'bioconda::mirdeep2=2.0.1.3'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/mirdeep2:2.0.1.3--hdfd78af_1' :
         'biocontainers/mirdeep2:2.0.1.3--hdfd78af_1' }"
diff --git a/modules/local/parse_fasta_mirna.nf b/modules/local/parse_fasta_mirna.nf
index ad63401e..60665251 100644
--- a/modules/local/parse_fasta_mirna.nf
+++ b/modules/local/parse_fasta_mirna.nf
@@ -1,10 +1,10 @@
 process PARSE_FASTA_MIRNA {
     label 'process_medium'
 
-    conda 'bioconda::seqkit=2.3.1'
+    conda 'bioconda::seqkit=2.6.1'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/seqkit:2.3.1--h9ee0642_0' :
-        'biocontainers/seqkit:2.3.1--h9ee0642_0' }"
+        'https://depot.galaxyproject.org/singularity/seqkit:2.6.1--h9ee0642_0' :
+        'biocontainers/seqkit:2.6.1--h9ee0642_0' }"
 
     input:
     tuple val(meta2), path(fasta)
diff --git a/modules/local/seqcluster_collapse.nf b/modules/local/seqcluster_collapse.nf
index 826f8cef..4379654c 100644
--- a/modules/local/seqcluster_collapse.nf
+++ b/modules/local/seqcluster_collapse.nf
@@ -2,7 +2,7 @@ process SEQCLUSTER_SEQUENCES {
     label 'process_medium'
     tag "$meta.id"
 
-    conda 'bioconda::seqcluster=1.2.9-0'
+    conda 'bioconda::seqcluster=1.2.9'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/seqcluster:1.2.9--pyh5e36f6f_0' :
         'biocontainers/seqcluster:1.2.9--pyh5e36f6f_0' }"

From 7eefccaa5d22d14ab9f9b4a5b6bd58a0d7300c8a Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 12:03:37 +0000
Subject: [PATCH 074/227] Fix

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 95e5b810..51a4a2cd 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -72,7 +72,7 @@ include { MIRNA_QUANT           } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT          } from '../subworkflows/local/genome_quant'
 include { MIRTRACE              } from '../subworkflows/local/mirtrace'
 include { MIRDEEP2              } from '../subworkflows/local/mirdeep2'
-include { INDEX_GENOME          } from '../../modules/local/bowtie_genome'
+include { INDEX_GENOME          } from '../modules/local/bowtie_genome'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From f118456bbfaa4e92a02cfc44f5bd109ca5e932b2 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 12:07:00 +0000
Subject: [PATCH 075/227] Fix

---
 modules/local/bowtie_genome.nf | 2 +-
 modules/local/bowtie_mirna.nf  | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/bowtie_genome.nf b/modules/local/bowtie_genome.nf
index 60f33f1a..17ea9253 100644
--- a/modules/local/bowtie_genome.nf
+++ b/modules/local/bowtie_genome.nf
@@ -5,7 +5,7 @@ process INDEX_GENOME {
     conda 'bioconda::bowtie=1.3.1'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/bowtie:1.3.1--py310h7b97f60_6' :
-        'biocontainers/bowtie:1.3.1-py310h30d4ff4_6' }"
+        'biocontainers/bowtie:1.3.1--py310h7b97f60_6' }"
 
     input:
     tuple val(meta2), path(fasta)
diff --git a/modules/local/bowtie_mirna.nf b/modules/local/bowtie_mirna.nf
index 2fbf1588..06756bef 100644
--- a/modules/local/bowtie_mirna.nf
+++ b/modules/local/bowtie_mirna.nf
@@ -4,7 +4,7 @@ process INDEX_MIRNA {
     conda 'bioconda::bowtie=1.3.1'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/bowtie:1.3.1--py310h7b97f60_6' :
-        'biocontainers/bowtie:1.3.1-py310h30d4ff4_6' }"
+        'biocontainers/bowtie:1.3.1--py310h30d4ff4_6' }"
 
     input:
     tuple val(meta2), path(fasta)

From cbbc6d8a307e4dea4c7c62f477250dce9467bbbb Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 12:16:56 +0000
Subject: [PATCH 076/227] Another fix

---
 modules/local/bowtie_mirna.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/bowtie_mirna.nf b/modules/local/bowtie_mirna.nf
index 06756bef..733d816e 100644
--- a/modules/local/bowtie_mirna.nf
+++ b/modules/local/bowtie_mirna.nf
@@ -4,7 +4,7 @@ process INDEX_MIRNA {
     conda 'bioconda::bowtie=1.3.1'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/bowtie:1.3.1--py310h7b97f60_6' :
-        'biocontainers/bowtie:1.3.1--py310h30d4ff4_6' }"
+        'biocontainers/bowtie:1.3.1--py310h7b97f60_6' }"
 
     input:
     tuple val(meta2), path(fasta)

From ea6d05eebd83cef1220f774b982f5e8a7236838f Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 12:39:04 +0000
Subject: [PATCH 077/227] Yeah fasta

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 51a4a2cd..b3de8acc 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -160,7 +160,7 @@ workflow SMRNASEQ {
             fasta_ch = file(params.fasta)
 
             //This needs to be done here as GENOME_QUANT should not run prior to the deduplication of UMIs.
-            INDEX_GENOME ( fasta )
+            INDEX_GENOME ( fasta_ch )
 
             ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)
 

From 6df081f83eadc4e48fe062ed6a802d683b6aa8ab Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 12:47:28 +0000
Subject: [PATCH 078/227] Another one in the umi workflow

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index b3de8acc..a9f34706 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -167,7 +167,7 @@ workflow SMRNASEQ {
             DEDUPLICATE_UMIS (
                 fasta_ch,
                 INDEX_GENOME.out.bowtie_indices,
-                FASTQC_UMITOOLS_FASTP.out.trim_reads
+                FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_reads
             )
             reads_for_mirna = DEDUPLICATE_UMIS.out.reads
             ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)

From 43d3aba11c6489baa9df94e1b0017338ea30ce6f Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 12 Jan 2024 20:39:33 +0000
Subject: [PATCH 079/227] Update edger_qc

---
 modules/local/edger_qc.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/edger_qc.nf b/modules/local/edger_qc.nf
index 5560de23..8c311457 100644
--- a/modules/local/edger_qc.nf
+++ b/modules/local/edger_qc.nf
@@ -3,8 +3,8 @@ process EDGER_QC {
 
     conda 'bioconda::bioconductor-limma=3.58.1 bioconda::bioconductor-edger=4.0.2 conda-forge::r-data.table=1.14.10 conda-forge::r-gplots=3.1.3 conda-forge::r-statmod=1.5.0'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-419bd7f10b2b902489ac63bbaafc7db76f8e0ae1:709335c37934db1b481054cbec637c6e5b5971cb-0' :
-        'biocontainers/mulled-v2-419bd7f10b2b902489ac63bbaafc7db76f8e0ae1:709335c37934db1b481054cbec637c6e5b5971cb-0' }"
+        'https://depot.galaxyproject.org/singularity/mulled-v2-419bd7f10b2b902489ac63bbaafc7db76f8e0ae1:f5ff7de321749bc7ae12f7e79a4b581497f4c8ce-0' :
+        'biocontainers/mulled-v2-419bd7f10b2b902489ac63bbaafc7db76f8e0ae1:f5ff7de321749bc7ae12f7e79a4b581497f4c8ce-0' }"
 
     input:
     path input_files

From 2059ed4cf72950cfb1e30000412b0e9aaf19163f Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 08:08:29 +0000
Subject: [PATCH 080/227] Shorten test.config

---
 conf/test.config | 4 +---
 1 file changed, 1 insertion(+), 3 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 450ef11d..e699776b 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -23,9 +23,7 @@ params {
 
     input            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/samplesheet/v2.0/samplesheet.csv'
     fasta            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.fa'
-    mature           = 'https://mirbase.org/download/mature.fa'
-    hairpin          = 'https://mirbase.org/download/hairpin.fa'
-    mirna_gtf        = 'https://mirbase.org/download/hsa.gff3'
+
     mirtrace_species = 'hsa'
     protocol         = 'illumina'
     skip_mirdeep     = true

From 945c3182d27150a0dcabd63b581d5573ac4fa595 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 08:10:00 +0000
Subject: [PATCH 081/227] Adding in UMI tests

---
 .github/workflows/ci.yml |  1 +
 conf/test_umi.config     | 31 +++++++++++++++++++++++++++++++
 nextflow.config          |  1 +
 3 files changed, 33 insertions(+)
 create mode 100644 conf/test_umi.config

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 885ae9b6..51c3ee37 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -29,6 +29,7 @@ jobs:
         profile:
           - "test"
           - "test_no_genome"
+          - "test_umi"
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v4
diff --git a/conf/test_umi.config b/conf/test_umi.config
new file mode 100644
index 00000000..a50ddb7a
--- /dev/null
+++ b/conf/test_umi.config
@@ -0,0 +1,31 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for running minimal tests
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines input files and everything required to run a fast and simple pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/smrnaseq -profile test,<docker/singularity> --outdir <OUTDIR>
+
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    config_profile_name        = 'Test profile'
+    config_profile_description = 'Minimal test dataset to check pipeline function'
+
+    // Limit resources so that this can run on GitHub Actions
+    max_cpus   = 2
+    max_memory = '6.GB'
+    max_time   = '6.h'
+
+    // Input data
+
+    input            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/samplesheet/v2.0/samplesheet_umi.csv'
+    fasta            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.fa'
+
+    mirtrace_species = 'hsa'
+    protocol         = 'illumina'
+    skip_mirdeep     = true
+
+}
diff --git a/nextflow.config b/nextflow.config
index 4143e3a0..b32d5ecf 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -230,6 +230,7 @@ profiles {
 
     }
     test           { includeConfig 'conf/test.config' }
+    test_umi       { includeConfig 'conf/test_umi.config' }
     test_no_genome { includeConfig 'conf/test_no_genome.config' }
     test_full      { includeConfig 'conf/test_full.config' }
 }

From e43e4cfcdf103c49588276c031d91a15bc9b132e Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 08:19:40 +0000
Subject: [PATCH 082/227] Addressing some reviews

---
 CHANGELOG.md                    |  1 +
 conf/modules.config             |  6 ++---
 subworkflows/local/umi_dedup.nf | 39 ++++++++++++++++-----------------
 3 files changed, 23 insertions(+), 23 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7ebcea04..611c5338 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [[#299]](https://github.com/nf-core/smrnaseq/issues/299) - Bugfix for missing inputs in BAM stats (`genome_quant.r`)
 - [[#164]](https://github.com/nf-core/smrnaseq/pull/164) - UMI Handling Feature implemented in the pipeline
 - [[#302]](https://github.com/nf-core/smrnaseq/pull/302) - Merged in nf-core template v2.11.1
+- [[#294]](https://github.com/nf-core/smrnaseq/pull/294) - Fixed contamination screening issues
 
 ### Parameters
 
diff --git a/conf/modules.config b/conf/modules.config
index b54a3534..769f62f8 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -131,7 +131,7 @@ if (!params.skip_fastp) {
 
     if (!params.skip_fastqc) {
         process {
-            withName: '.*:.*:FASTQC_FASTP:FASTQC_RAW' {
+            withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_RAW' {
                 ext.args = '--quiet'
                 publishDir = [
                     path: { "${params.outdir}/fastqc/raw" },
@@ -139,7 +139,7 @@ if (!params.skip_fastp) {
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: '.*:.*:FASTQC_FASTP:FASTQC_TRIM' {
+            withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' {
                 ext.args = '--quiet'
                 publishDir = [
                     path: { "${params.outdir}/fastqc/trim" },
@@ -153,7 +153,7 @@ if (!params.skip_fastp) {
 
 if (params.with_umi && !params.skip_umi_extract) {
     process {
-        withName: '.*:FASTQC_UMITOOLS_TRIMGALORE:UMITOOLS_EXTRACT' {
+        withName: '.*:FASTQC_UMITOOLS_FASTP:UMITOOLS_EXTRACT' {
             ext.args   = [
                     params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
                     params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 5a5a393f..db9065ea 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -31,36 +31,35 @@ workflow DEDUPLICATE_UMIS {
         fasta_formatted = fasta
     }
 
-    if (bt_index){
 
-        UMI_MAP_GENOME ( reads, bt_index.collect() )
-        ch_versions = ch_versions.mix(UMI_MAP_GENOME.out.versions)
+    UMI_MAP_GENOME ( reads, bt_index.collect() )
+    ch_versions = ch_versions.mix(UMI_MAP_GENOME.out.versions)
 
-        BAM_SORT_SAMTOOLS ( UMI_MAP_GENOME.out.bam, Channel.empty() )
-        ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions)
+    BAM_SORT_SAMTOOLS ( UMI_MAP_GENOME.out.bam, Channel.empty() )
+    ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions)
 
-        ch_umi_dedup = BAM_SORT_SAMTOOLS.out.bam.join(BAM_SORT_SAMTOOLS.out.bai)
-        UMITOOLS_DEDUP ( ch_umi_dedup )
-        ch_versions = ch_versions.mix(UMITOOLS_DEDUP.out.versions)
-        ch_dedup_stats = ch_dedup_stats.mix(UMITOOLS_DEDUP.out.tsv_edit_distance).join(UMITOOLS_DEDUP.out.tsv_per_umi).join(UMITOOLS_DEDUP.out.tsv_umi_per_position)
+    ch_umi_dedup = BAM_SORT_SAMTOOLS.out.bam.join(BAM_SORT_SAMTOOLS.out.bai)
+    UMITOOLS_DEDUP ( ch_umi_dedup )
+    ch_versions = ch_versions.mix(UMITOOLS_DEDUP.out.versions)
+    ch_dedup_stats = ch_dedup_stats.mix(UMITOOLS_DEDUP.out.tsv_edit_distance).join(UMITOOLS_DEDUP.out.tsv_per_umi).join(UMITOOLS_DEDUP.out.tsv_umi_per_position)
 
-        SAMTOOLS_BAM2FQ ( UMITOOLS_DEDUP.out.bam, false )
-        ch_versions = ch_versions.mix(SAMTOOLS_BAM2FQ.out.versions)
+    SAMTOOLS_BAM2FQ ( UMITOOLS_DEDUP.out.bam, false )
+    ch_versions = ch_versions.mix(SAMTOOLS_BAM2FQ.out.versions)
 
-        ch_dedup_reads = SAMTOOLS_BAM2FQ.out.reads
+    ch_dedup_reads = SAMTOOLS_BAM2FQ.out.reads
 
-        if ( params.umi_merge_unmapped ) {
+    if ( params.umi_merge_unmapped ) {
 
-            SAMTOOLS_BAM2FQ.out.reads
-                .join(UMI_MAP_GENOME.out.unmapped)
-                .map { meta, file1, file2 -> [meta, [file1, file2]]}
-                .set { ch_cat }
+        SAMTOOLS_BAM2FQ.out.reads
+            .join(UMI_MAP_GENOME.out.unmapped)
+            .map { meta, file1, file2 -> [meta, [file1, file2]]}
+            .set { ch_cat }
 
-            CAT_CAT ( ch_cat )
-            ch_dedup_reads = CAT_CAT.out.file_out
-        }
+        CAT_CAT ( ch_cat )
+        ch_dedup_reads = CAT_CAT.out.file_out
     }
 
+
     emit:
     reads    = ch_dedup_reads
     indices  = bt_index

From 3b21fe1a504dc7229d2f49d33bc7d570351bf617 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 08:39:29 +0000
Subject: [PATCH 083/227] Add UMI regex

---
 conf/test_umi.config | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/conf/test_umi.config b/conf/test_umi.config
index a50ddb7a..a7a59e75 100644
--- a/conf/test_umi.config
+++ b/conf/test_umi.config
@@ -28,4 +28,10 @@ params {
     protocol         = 'illumina'
     skip_mirdeep     = true
 
+    //UMI Specific testcase
+    with_umi = true
+    umitools_extract_method = 'regex'
+    umitools_bc_pattern '.+AACTGTAGGCACCATCAAT{s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
+    save_umi_intermeds = true
+
 }

From 8e21df8efa67c7e103fc34d5239c6e9a1a716892 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 08:57:18 +0000
Subject: [PATCH 084/227] Some more adjustments to make nf-core modules work

---
 conf/test_umi.config            | 2 +-
 nextflow.config                 | 1 +
 nextflow_schema.json            | 5 +++++
 subworkflows/local/umi_dedup.nf | 9 +++++----
 workflows/smrnaseq.nf           | 3 ++-
 5 files changed, 14 insertions(+), 6 deletions(-)

diff --git a/conf/test_umi.config b/conf/test_umi.config
index a7a59e75..53a58f41 100644
--- a/conf/test_umi.config
+++ b/conf/test_umi.config
@@ -31,7 +31,7 @@ params {
     //UMI Specific testcase
     with_umi = true
     umitools_extract_method = 'regex'
-    umitools_bc_pattern '.+AACTGTAGGCACCATCAAT{s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
+    umitools_bc_pattern = '.+AACTGTAGGCACCATCAAT{s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
     save_umi_intermeds = true
 
 }
diff --git a/nextflow.config b/nextflow.config
index b32d5ecf..0a6d0945 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -38,6 +38,7 @@ params {
     umi_discard_read           = null
     save_umi_intermeds         = false
     umi_merge_unmapped         = true
+    umi_stats                  = true
 
     // Trimming options
     clip_r1                     = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index a8eebb26..cd8fa7a5 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -94,6 +94,11 @@
                     "fa_icon": "fas fa-save",
                     "description": "Unless deactivated the deduplicated reads are merged with the reads that could not be placed to reduce the potential reference bias",
                     "default": true
+                },
+                "umi_stats": {
+                    "type": "boolean",
+                    "default": true,
+                    "description": "Compute UMI statistics for MultiQC"
                 }
             },
             "fa_icon": "fas fa-barcode"
diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index db9065ea..1c88cde0 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -15,6 +15,7 @@ workflow DEDUPLICATE_UMIS {
     fasta
     bt_index
     reads      // channel: [ val(meta), [ reads ] ]
+    val_get_dedup_stats //boolean true/false
 
     main:
 
@@ -35,11 +36,11 @@ workflow DEDUPLICATE_UMIS {
     UMI_MAP_GENOME ( reads, bt_index.collect() )
     ch_versions = ch_versions.mix(UMI_MAP_GENOME.out.versions)
 
-    BAM_SORT_SAMTOOLS ( UMI_MAP_GENOME.out.bam, Channel.empty() )
-    ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions)
+    BAM_SORT_STATS_SAMTOOLS ( UMI_MAP_GENOME.out.bam, Channel.empty() )
+    ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions)
 
-    ch_umi_dedup = BAM_SORT_SAMTOOLS.out.bam.join(BAM_SORT_SAMTOOLS.out.bai)
-    UMITOOLS_DEDUP ( ch_umi_dedup )
+    //ch_umi_dedup = BAM_SORT_STATS_SAMTOOLS.out.bam.join(BAM_SORT_STATS_SAMTOOLS.out.bai)
+    UMITOOLS_DEDUP ( BAM_SORT_STATS_SAMTOOLS.out.bam, val_get_dedup_stats)
     ch_versions = ch_versions.mix(UMITOOLS_DEDUP.out.versions)
     ch_dedup_stats = ch_dedup_stats.mix(UMITOOLS_DEDUP.out.tsv_edit_distance).join(UMITOOLS_DEDUP.out.tsv_per_umi).join(UMITOOLS_DEDUP.out.tsv_umi_per_position)
 
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index a9f34706..cb46b44d 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -167,7 +167,8 @@ workflow SMRNASEQ {
             DEDUPLICATE_UMIS (
                 fasta_ch,
                 INDEX_GENOME.out.bowtie_indices,
-                FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_reads
+                FASTQ_FASTQC_UMITOOLS_FASTP.out.reads,
+                params.umi_stats
             )
             reads_for_mirna = DEDUPLICATE_UMIS.out.reads
             ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)

From fc0119558de7a6a73f85241000fda4fa368a828b Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 09:00:44 +0000
Subject: [PATCH 085/227] Fix meta2

---
 subworkflows/local/umi_dedup.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 1c88cde0..3d12fd99 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -23,7 +23,7 @@ workflow DEDUPLICATE_UMIS {
     ch_dedup_stats = Channel.empty()
 
     if (!bt_index){
-        INDEX_GENOME ( fasta )
+        INDEX_GENOME ( [ [:], fasta ] )
         bt_index      = INDEX_GENOME.out.bowtie_indices
         fasta_formatted = INDEX_GENOME.out.fasta
         ch_versions     = ch_versions.mix(INDEX_GENOME.out.versions)

From 14109235504f62bd912373d785af21027c96a4ca Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 09:05:31 +0000
Subject: [PATCH 086/227] Passing on meta2

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index cb46b44d..ad291593 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -160,7 +160,7 @@ workflow SMRNASEQ {
             fasta_ch = file(params.fasta)
 
             //This needs to be done here as GENOME_QUANT should not run prior to the deduplication of UMIs.
-            INDEX_GENOME ( fasta_ch )
+            INDEX_GENOME ( [ [:], fasta ] )
 
             ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)
 

From 12be1865b66035915f7b1086a723817b60bf5d2b Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 09:05:49 +0000
Subject: [PATCH 087/227] use the channels, luke

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index ad291593..872de5f3 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -160,7 +160,7 @@ workflow SMRNASEQ {
             fasta_ch = file(params.fasta)
 
             //This needs to be done here as GENOME_QUANT should not run prior to the deduplication of UMIs.
-            INDEX_GENOME ( [ [:], fasta ] )
+            INDEX_GENOME ( [ [:], fasta_ch ] )
 
             ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)
 

From 96adf135bedb6ed20394537b9915ca38cb89af01 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 20:42:21 +0000
Subject: [PATCH 088/227] Add better changelog with deps

---
 CHANGELOG.md | 15 ++++++++++++++-
 1 file changed, 14 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 611c5338..fc286f02 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,6 +21,20 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 |               | `--save_umi_intermeds`      |
 |               | `--umi_merge_unmapped`      |
 
+### Software dependencies
+
+| Dependency   | Old version | New version |
+| ------------ | ----------- | ----------- |
+| `multiqc`    | 1.15        | 1.19        |
+| `edgeR`      | 3.36.0      | 4.0.2       |
+| `limma`      | 3.50.0      | 3.58.1      |
+| `bioconvert` | 0.4.3       | 1.1.1       |
+| `mirdeep`    | 2.0.1       | 2.0.1.3     |
+| `seqkit`     | 2.3.1       | 2.6.1       |
+| `fastqc`     | 0.11.4      | 0.12.1      |
+| `samtools`   | 1.17        | 1.18        |
+| `umitools`   | <none>      | 1.1.4       |
+
 ## [v2.2.4](https://github.com/nf-core/smrnaseq/releases/tag/2.2.4) - 2023-11-03
 
 - Update template to 2.10
@@ -119,7 +133,6 @@ Note, since the pipeline is now using Nextflow DSL2, each process will be run wi
 | `seqkit`             | 0.16.0      | 2.0.0       |
 | `trim-galore`        | 0.6.6       | 0.6.7       |
 | `bioconvert`         | -           | 0.4.3       |
-| `umi_tools`          | -           | 1.1.2       |
 | `htseq`              | -           | -           |
 | `markdown`           | -           | -           |
 | `pymdown-extensions` | -           | -           |

From 5c7227a30de536d24228ff3b516213f852a05065 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 20:42:44 +0000
Subject: [PATCH 089/227] Add updated modules

---
 modules.json                                  |   8 +-
 modules/nf-core/cat/cat/main.nf               |  11 +-
 modules/nf-core/cat/cat/tests/main.nf.test    |   6 +-
 .../nf-core/cat/cat/tests/main.nf.test.snap   |  92 +++++----
 modules/nf-core/cat/fastq/tests/main.nf.test  |  63 +++---
 .../nf-core/cat/fastq/tests/main.nf.test.snap | 185 +++++++++++++-----
 modules/nf-core/fastp/tests/main.nf.test      |  95 +++++----
 modules/nf-core/fastp/tests/main.nf.test.snap |  16 +-
 8 files changed, 295 insertions(+), 181 deletions(-)

diff --git a/modules.json b/modules.json
index 40eda6cc..f11503fc 100644
--- a/modules.json
+++ b/modules.json
@@ -7,12 +7,12 @@
                 "nf-core": {
                     "cat/cat": {
                         "branch": "master",
-                        "git_sha": "d593e8f6b7d1bbbb2acf43a4b9efeeac8d6720f2",
+                        "git_sha": "81f27e75847087865299cc46605deb3b09b4e0a2",
                         "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "02fd5bd7275abad27aad32d5c852e0a9b1b98882",
                         "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
@@ -22,13 +22,13 @@
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "d086322563bdbb08c94bf15a7db58a39ccdb1520",
+                        "git_sha": "1799e452de650f6fb8890d25829bca23014b0728",
                         "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "617777a807a1770f73deb38c80004bac06807eef",
-                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
+                        "installed_by": ["fastq_fastqc_umitools_fastp"]
                     },
                     "multiqc": {
                         "branch": "master",
diff --git a/modules/nf-core/cat/cat/main.nf b/modules/nf-core/cat/cat/main.nf
index 970ab760..adbdbd7b 100644
--- a/modules/nf-core/cat/cat/main.nf
+++ b/modules/nf-core/cat/cat/main.nf
@@ -22,6 +22,8 @@ process CAT_CAT {
     def args2 = task.ext.args2 ?: ''
     def file_list = files_in.collect { it.toString() }
 
+    // choose appropriate concatenation tool depending on input and output format
+
     // | input     | output     | command1 | command2 |
     // |-----------|------------|----------|----------|
     // | gzipped   | gzipped    | cat      |          |
@@ -30,7 +32,7 @@ process CAT_CAT {
     // | ungzipped | gzipped    | cat      | pigz     |
 
     // Use input file ending as default
-    prefix   = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
+    prefix   = task.ext.prefix ?: "${meta.id}${getFileSuffix(file_list[0])}"
     out_zip  = prefix.endsWith('.gz')
     in_zip   = file_list[0].endsWith('.gz')
     command1 = (in_zip && !out_zip) ? 'zcat' : 'cat'
@@ -68,3 +70,10 @@ process CAT_CAT {
     END_VERSIONS
     """
 }
+
+// for .gz files also include the second to last extension if it is present. E.g., .fasta.gz
+def getFileSuffix(filename) {
+    def match = filename =~ /^.*?((\.\w{1,5})?(\.\w{1,5}\.gz$))/
+    return match ? match[0][1] : filename.substring(filename.lastIndexOf('.'))
+}
+
diff --git a/modules/nf-core/cat/cat/tests/main.nf.test b/modules/nf-core/cat/cat/tests/main.nf.test
index ed5a4f12..aaae04f9 100644
--- a/modules/nf-core/cat/cat/tests/main.nf.test
+++ b/modules/nf-core/cat/cat/tests/main.nf.test
@@ -83,8 +83,7 @@ nextflow_process {
             def lines = path(process.out.file_out.get(0).get(1)).linesGzip
             assertAll(
                 { assert process.success },
-                { assert snapshot(lines[0..5]).match("test_cat_zipped_zipped_lines") },
-                { assert snapshot(lines.size()).match("test_cat_zipped_zipped_size")}
+                { assert snapshot(process.out).match() }
             )
         }
     }
@@ -142,8 +141,7 @@ nextflow_process {
             def lines = path(process.out.file_out.get(0).get(1)).linesGzip
             assertAll(
                 { assert process.success },
-                { assert snapshot(lines[0..5]).match("test_cat_unzipped_zipped_lines") },
-                { assert snapshot(lines.size()).match("test_cat_unzipped_zipped_size")}
+                { assert snapshot(process.out).match() }
             )
         }
     }
diff --git a/modules/nf-core/cat/cat/tests/main.nf.test.snap b/modules/nf-core/cat/cat/tests/main.nf.test.snap
index 423571ba..0c9bfe8d 100644
--- a/modules/nf-core/cat/cat/tests/main.nf.test.snap
+++ b/modules/nf-core/cat/cat/tests/main.nf.test.snap
@@ -1,10 +1,4 @@
 {
-    "test_cat_unzipped_zipped_size": {
-        "content": [
-            375
-        ],
-        "timestamp": "2023-10-16T14:33:08.049445686"
-    },
     "test_cat_unzipped_unzipped": {
         "content": [
             {
@@ -67,31 +61,36 @@
         ],
         "timestamp": "2023-10-16T14:32:49.642741302"
     },
-    "test_cat_zipped_zipped_lines": {
-        "content": [
-            [
-                "MT192765.1\tGenbank\ttranscript\t259\t29667\t.\t+\t.\tID=unknown_transcript_1;geneID=orf1ab;gene_name=orf1ab",
-                "MT192765.1\tGenbank\tgene\t259\t21548\t.\t+\t.\tParent=unknown_transcript_1",
-                "MT192765.1\tGenbank\tCDS\t259\t13461\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1",
-                "MT192765.1\tGenbank\tCDS\t13461\t21548\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1",
-                "MT192765.1\tGenbank\tCDS\t21556\t25377\t.\t+\t0\tParent=unknown_transcript_1;gbkey=CDS;gene=S;note=\"structural protein\";product=\"surface glycoprotein\";protein_id=QIK50427.1",
-                "MT192765.1\tGenbank\tgene\t21556\t25377\t.\t+\t.\tParent=unknown_transcript_1"
-            ]
-        ],
-        "timestamp": "2023-10-16T14:32:33.629048645"
-    },
-    "test_cat_unzipped_zipped_lines": {
+    "test_cat_zipped_zipped": {
         "content": [
-            [
-                ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome",
-                "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT",
-                "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG",
-                "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG",
-                "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT",
-                "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG"
-            ]
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
+                ],
+                "file_out": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
+                ]
+            }
         ],
-        "timestamp": "2023-10-16T14:33:08.038830506"
+        "timestamp": "2024-01-12T14:02:02.999254641"
     },
     "test_cat_one_file_unzipped_zipped_lines": {
         "content": [
@@ -106,16 +105,41 @@
         ],
         "timestamp": "2023-10-16T14:33:21.39642399"
     },
-    "test_cat_zipped_zipped_size": {
+    "test_cat_unzipped_zipped": {
         "content": [
-            78
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
+                ],
+                "file_out": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
+                ]
+            }
         ],
-        "timestamp": "2023-10-16T14:32:33.641869244"
+        "timestamp": "2024-01-12T14:08:26.948048418"
     },
     "test_cat_one_file_unzipped_zipped_size": {
         "content": [
             374
         ],
-        "timestamp": "2023-10-16T14:33:21.4094373"
+        "timestamp": "2024-01-12T14:10:22.445700266"
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test b/modules/nf-core/cat/fastq/tests/main.nf.test
index f5f94182..dab2e14c 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test
@@ -16,11 +16,11 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                            [ id:'test', single_end:true ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                            file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true) ]
-                        ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -28,8 +28,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
@@ -42,13 +41,13 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -56,8 +55,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
@@ -70,11 +68,11 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:true ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -82,8 +80,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
@@ -96,13 +93,13 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -110,8 +107,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
@@ -124,10 +120,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:true ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -135,8 +131,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test.snap b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
index ec2342e5..43dfe28f 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test.snap
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
@@ -1,78 +1,169 @@
 {
     "test_cat_fastq_single_end": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": true
-                    },
-                    "test.merged.fastq.gz:md5,f9cf5e375f7de81a406144a2c70cc64d"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-17T23:19:12.990284837"
+        "timestamp": "2024-01-17T17:30:39.816981"
     },
     "test_cat_fastq_single_end_same_name": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": true
-                    },
-                    "test.merged.fastq.gz:md5,63f817db7a29a03eb538104495556f66"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-17T23:19:31.554568147"
+        "timestamp": "2024-01-17T17:32:35.229332"
     },
     "test_cat_fastq_single_end_single_file": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": true
-                    },
-                    "test.merged.fastq.gz:md5,e325ef7deb4023447a1f074e285761af"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-17T23:19:49.629360033"
+        "timestamp": "2024-01-17T17:34:00.058829"
     },
     "test_cat_fastq_paired_end_same_name": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
+            {
+                "0": [
                     [
-                        "test_1.merged.fastq.gz:md5,63f817db7a29a03eb538104495556f66",
-                        "test_2.merged.fastq.gz:md5,fe9f266f43a6fc3dcab690a18419a56e"
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
                     ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-17T23:19:40.711617539"
+        "timestamp": "2024-01-17T17:33:33.031555"
     },
     "test_cat_fastq_paired_end": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
                     [
-                        "test_1.merged.fastq.gz:md5,f9cf5e375f7de81a406144a2c70cc64d",
-                        "test_2.merged.fastq.gz:md5,77c8e966e130d8c6b6ec9be52fcb2bda"
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
                     ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-18T07:53:20.923560211"
+        "timestamp": "2024-01-17T17:32:02.270935"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test
index 17dce8ac..dcf4fd6f 100644
--- a/modules/nf-core/fastp/tests/main.nf.test
+++ b/modules/nf-core/fastp/tests/main.nf.test
@@ -19,11 +19,10 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = false
 
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:true ],
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
-                ]
-
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -89,11 +88,10 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = false
 
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:true ],
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
-                ]
-
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -135,12 +133,11 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = false
 
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                ]
-
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -220,12 +217,11 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = false
 
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                ]
-
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -266,10 +262,10 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = false
 
-                input[0] = [ [ id:'test', single_end:true ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ]
-                        ]
-
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -336,10 +332,10 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = false
 
-                input[0] = [ [ id:'test', single_end:true ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ]
-                        ]
-
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -380,9 +376,10 @@ nextflow_process {
                 save_trimmed_fail = true
                 save_merged       = false
 
-                input[0] = [ [ id:'test', single_end:true ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
-                        ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -440,13 +437,11 @@ nextflow_process {
                 save_trimmed_fail = true
                 save_merged       = false
 
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-                    ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -519,11 +514,11 @@ nextflow_process {
                 adapter_fasta     = []
                 save_trimmed_fail = false
                 save_merged       = true
-
-                input[0] = [ [ id:'test', single_end:false ], // meta map
-                             [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                               file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                           ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -613,10 +608,11 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = true
 
-                input[0] = [ [ id:'test', single_end:false ], // meta map
-                             [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                               file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                           ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -653,14 +649,15 @@ nextflow_process {
             }
             process {
                 """
-                adapter_fasta     = file("https://github.com/nf-core/test-datasets/raw/modules/data/delete_me/fastp/adapters.fasta", checkIfExists: true)
+                adapter_fasta     = Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ])
                 save_trimmed_fail = false
                 save_merged       = true
 
-                input[0] = [ [ id:'test', single_end:false ], // meta map
-                             [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                               file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                           ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap
index 1b7d2419..6a71b680 100644
--- a/modules/nf-core/fastp/tests/main.nf.test.snap
+++ b/modules/nf-core/fastp/tests/main.nf.test.snap
@@ -12,7 +12,7 @@
                 "{id=test, single_end=false}"
             ]
         ],
-        "timestamp": "2023-12-21T09:44:37.202512"
+        "timestamp": "2024-01-17T18:07:15.398827"
     },
     "fastp test_fastp_interleaved_json": {
         "content": [
@@ -26,7 +26,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-10-17T11:04:45.794175881"
+        "timestamp": "2024-01-17T18:08:06.123035"
     },
     "test_fastp_paired_end_merged-for_stub_match": {
         "content": [
@@ -42,7 +42,7 @@
                 "{id=test, single_end=false}"
             ]
         ],
-        "timestamp": "2023-12-21T09:53:45.237014"
+        "timestamp": "2024-01-17T18:10:13.467574"
     },
     "test_fastp_single_end_json": {
         "content": [
@@ -56,7 +56,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-10-17T11:04:10.566343705"
+        "timestamp": "2024-01-17T18:06:00.223817"
     },
     "versions": {
         "content": [
@@ -64,7 +64,7 @@
                 "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
             ]
         ],
-        "timestamp": "2023-10-17T11:04:10.582076024"
+        "timestamp": "2024-01-17T18:06:00.248422"
     },
     "test_fastp_interleaved-for_stub_match": {
         "content": [
@@ -76,7 +76,7 @@
                 "{id=test, single_end=true}"
             ]
         ],
-        "timestamp": "2023-12-21T09:48:43.148485"
+        "timestamp": "2024-01-17T18:08:06.127974"
     },
     "test_fastp_single_end-for_stub_match": {
         "content": [
@@ -88,7 +88,7 @@
                 "{id=test, single_end=true}"
             ]
         ],
-        "timestamp": "2023-12-21T09:20:07.254788"
+        "timestamp": "2024-01-17T18:06:00.244202"
     },
     "test_fastp_single_end_trim_fail_json": {
         "content": [
@@ -102,6 +102,6 @@
                 ]
             ]
         ],
-        "timestamp": "2023-10-17T11:05:00.379878948"
+        "timestamp": "2024-01-17T18:08:41.942317"
     }
 }
\ No newline at end of file

From 8d19dbf47604d5cd2acd898586b23d093102766f Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 20:43:07 +0000
Subject: [PATCH 090/227] Add updated modules.json

---
 modules.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules.json b/modules.json
index f11503fc..9d68299f 100644
--- a/modules.json
+++ b/modules.json
@@ -91,7 +91,7 @@
                     },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
-                        "git_sha": "668185ddcd2d9084c819691c99020360e0f029a0",
+                        "git_sha": "d20574ce7420a54fe47b46a91c5f2215d1a2777c",
                         "installed_by": ["subworkflows"]
                     }
                 }

From 84da917bc464f02147a40515ebad4942880a4d2c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 17 Jan 2024 20:53:29 +0000
Subject: [PATCH 091/227] Fixing the error :)

---
 subworkflows/local/umi_dedup.nf | 3 +--
 workflows/smrnaseq.nf           | 2 +-
 2 files changed, 2 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 3d12fd99..4a7f80f9 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -24,11 +24,10 @@ workflow DEDUPLICATE_UMIS {
 
     if (!bt_index){
         INDEX_GENOME ( [ [:], fasta ] )
-        bt_index      = INDEX_GENOME.out.bowtie_indices
+        bt_index      = INDEX_GENOME.out.index
         fasta_formatted = INDEX_GENOME.out.fasta
         ch_versions     = ch_versions.mix(INDEX_GENOME.out.versions)
     } else {
-        bt_index     = Channel.fromPath("${bt_index}**ebwt", checkIfExists: true).ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_index}" }
         fasta_formatted = fasta
     }
 
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 872de5f3..02501098 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -166,7 +166,7 @@ workflow SMRNASEQ {
 
             DEDUPLICATE_UMIS (
                 fasta_ch,
-                INDEX_GENOME.out.bowtie_indices,
+                INDEX_GENOME.out.index,
                 FASTQ_FASTQC_UMITOOLS_FASTP.out.reads,
                 params.umi_stats
             )

From e95f075895e77325244073017c20b474bb547730 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 18 Jan 2024 07:58:51 +0000
Subject: [PATCH 092/227] [automated] Fix linting with Prettier

---
 .devcontainer/devcontainer.json | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json
index 4ecfbfe3..4a9bc5c7 100644
--- a/.devcontainer/devcontainer.json
+++ b/.devcontainer/devcontainer.json
@@ -18,11 +18,11 @@
                 "python.linting.flake8Path": "/opt/conda/bin/flake8",
                 "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle",
                 "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle",
-                "python.linting.pylintPath": "/opt/conda/bin/pylint"
+                "python.linting.pylintPath": "/opt/conda/bin/pylint",
             },
 
             // Add the IDs of extensions you want installed when the container is created.
-            "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"]
-        }
-    }
+            "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"],
+        },
+    },
 }

From c51f54d491fbcb192c270ebad97da5b5a0d8c5b1 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 18 Jan 2024 08:21:09 +0000
Subject: [PATCH 093/227] This should fix umitools extract

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index 769f62f8..6e6820f5 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -153,7 +153,7 @@ if (!params.skip_fastp) {
 
 if (params.with_umi && !params.skip_umi_extract) {
     process {
-        withName: '.*:FASTQC_UMITOOLS_FASTP:UMITOOLS_EXTRACT' {
+        withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:UMITOOLS_EXTRACT' {
             ext.args   = [
                     params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
                     params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',

From 3776e1834a37c5a3d32df3ebbceb5f89d1312bc4 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 18 Jan 2024 08:29:52 +0000
Subject: [PATCH 094/227] Improve more modules

---
 conf/modules.config | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 6e6820f5..23406e58 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -201,7 +201,7 @@ if (params.with_umi) {
             ]
         }
 
-        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT' {
+        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' {
             ext.prefix = { "${meta.id}.sorted" }
             publishDir = [
                 path: { "${params.outdir}/umi_dedup" },
@@ -213,7 +213,7 @@ if (params.with_umi) {
             ]
         }
 
-        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX' {
+        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX' {
             ext.prefix = { "${meta.id}.sorted" }
             publishDir = [
                 path: { "${params.outdir}/umi_dedup" },
@@ -225,7 +225,7 @@ if (params.with_umi) {
             ]
         }
 
-        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:.*' {
+        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:.*' {
             publishDir = [
                 path: { "${params.outdir}/umi_dedup/samtools_stats" },
                 mode: params.publish_dir_mode,

From a5daf6b74ffe4649403b6921e2c6aae88aec357d Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 18 Jan 2024 08:30:52 +0000
Subject: [PATCH 095/227] Less lines

---
 subworkflows/local/mirna_quant.nf | 10 ----------
 1 file changed, 10 deletions(-)

diff --git a/subworkflows/local/mirna_quant.nf b/subworkflows/local/mirna_quant.nf
index e26839bc..fc6942fe 100644
--- a/subworkflows/local/mirna_quant.nf
+++ b/subworkflows/local/mirna_quant.nf
@@ -33,8 +33,6 @@ workflow MIRNA_QUANT {
     main:
     ch_versions = Channel.empty()
 
-
-
     PARSE_MATURE ( mature ).parsed_fasta.set { mirna_parsed }
     ch_versions = ch_versions.mix(PARSE_MATURE.out.versions)
 
@@ -78,7 +76,6 @@ workflow MIRNA_QUANT {
     ch_versions = ch_versions.mix(BAM_STATS_HAIRPIN.out.versions)
 
 
-
     BAM_STATS_MATURE.out.idxstats.collect{it[1]}
         .mix(BAM_STATS_HAIRPIN.out.idxstats.collect{it[1]})
         .dump(tag:'edger')
@@ -87,8 +84,6 @@ workflow MIRNA_QUANT {
         .set { edger_input }
     EDGER_QC ( edger_input )
 
-
-
     reads
         .map { add_suffix(it, "seqcluster") }
         .dump (tag:'ssux')
@@ -100,9 +95,6 @@ workflow MIRNA_QUANT {
     BOWTIE_MAP_SEQCLUSTER ( reads_collapsed, hairpin_bowtie.collect() )
     ch_versions = ch_versions.mix(BOWTIE_MAP_SEQCLUSTER.out.versions)
 
-
-
-
     ch_mirtop_logs = Channel.empty()
     if (params.mirtrace_species){
         MIRTOP_QUANT ( BOWTIE_MAP_SEQCLUSTER.out.bam.collect{it[1]}, FORMAT_HAIRPIN.out.formatted_fasta.collect{it[1]}, gtf )
@@ -117,8 +109,6 @@ workflow MIRNA_QUANT {
         .dump (tag:'gsux')
         .set { reads_genome }
 
-
-
     emit:
     fasta_mature        = FORMAT_MATURE.out.formatted_fasta
     fasta_hairpin       = FORMAT_HAIRPIN.out.formatted_fasta

From 4baf752461389e77f0bc7b9c036ebd40283596e2 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <apeltzer@users.noreply.github.com>
Date: Thu, 18 Jan 2024 09:31:57 +0100
Subject: [PATCH 096/227] Apply suggestions from code review

Co-authored-by: Maxime U Garcia <max.u.garcia@gmail.com>
---
 nextflow.config       |  8 ++++----
 workflows/smrnaseq.nf | 16 ++++++++--------
 2 files changed, 12 insertions(+), 12 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 0a6d0945..4d7eb423 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -247,8 +247,6 @@ singularity.registry = 'quay.io'
 // Nextflow plugins
 plugins {
 
-    id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet
-
     id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet
 }
 
@@ -276,8 +274,10 @@ process.shell = ['/bin/bash', '-euo', 'pipefail']
 // Set default registry for Docker and Podman independent of -profile
 // Will not be used unless Docker / Podman are enabled
 // Set to your registry if you have a mirror of containers
-docker.registry = 'quay.io'
-podman.registry = 'quay.io'
+apptainer.registry   = 'quay.io'
+docker.registry      = 'quay.io'
+podman.registry      = 'quay.io'
+singularity.registry = 'quay.io'
 
 // Disable process selector warnings by default. Use debug profile to enable warnings.
 nextflow.enable.configProcessNamesValidation = false
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 02501098..4b81796c 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -64,15 +64,15 @@ if (!params.mirgenedb) {
     if (params.mirgenedb_gff) { mirna_gtf = file(params.mirgenedb_gff, checkIfExists: true) } else { exit 1, "MirGeneDB gff file not found: ${params.mirgenedb_gff}"}
 }
 
-include { INPUT_CHECK           } from '../subworkflows/local/input_check'
+include { INPUT_CHECK                 } from '../subworkflows/local/input_check'
 include { FASTQ_FASTQC_UMITOOLS_FASTP } from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp'
-include { DEDUPLICATE_UMIS      } from '../subworkflows/local/umi_dedup'
-include { CONTAMINANT_FILTER    } from '../subworkflows/local/contaminant_filter'
-include { MIRNA_QUANT           } from '../subworkflows/local/mirna_quant'
-include { GENOME_QUANT          } from '../subworkflows/local/genome_quant'
-include { MIRTRACE              } from '../subworkflows/local/mirtrace'
-include { MIRDEEP2              } from '../subworkflows/local/mirdeep2'
-include { INDEX_GENOME          } from '../modules/local/bowtie_genome'
+include { DEDUPLICATE_UMIS            } from '../subworkflows/local/umi_dedup'
+include { CONTAMINANT_FILTER          } from '../subworkflows/local/contaminant_filter'
+include { MIRNA_QUANT                 } from '../subworkflows/local/mirna_quant'
+include { GENOME_QUANT                } from '../subworkflows/local/genome_quant'
+include { MIRTRACE                    } from '../subworkflows/local/mirtrace'
+include { MIRDEEP2                    } from '../subworkflows/local/mirdeep2'
+include { INDEX_GENOME                } from '../modules/local/bowtie_genome'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From 533a0f35e6e78d41b496f12af4f6004263a3b20f Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Thu, 18 Jan 2024 10:16:06 +0100
Subject: [PATCH 097/227] Fix input cardinality

---
 subworkflows/local/umi_dedup.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 4a7f80f9..f8981758 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -38,8 +38,8 @@ workflow DEDUPLICATE_UMIS {
     BAM_SORT_STATS_SAMTOOLS ( UMI_MAP_GENOME.out.bam, Channel.empty() )
     ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions)
 
-    //ch_umi_dedup = BAM_SORT_STATS_SAMTOOLS.out.bam.join(BAM_SORT_STATS_SAMTOOLS.out.bai)
-    UMITOOLS_DEDUP ( BAM_SORT_STATS_SAMTOOLS.out.bam, val_get_dedup_stats)
+    ch_umi_dedup = BAM_SORT_STATS_SAMTOOLS.out.bam.join(BAM_SORT_STATS_SAMTOOLS.out.bai)
+    UMITOOLS_DEDUP ( ch_umi_dedup, val_get_dedup_stats)
     ch_versions = ch_versions.mix(UMITOOLS_DEDUP.out.versions)
     ch_dedup_stats = ch_dedup_stats.mix(UMITOOLS_DEDUP.out.tsv_edit_distance).join(UMITOOLS_DEDUP.out.tsv_per_umi).join(UMITOOLS_DEDUP.out.tsv_umi_per_position)
 

From 60052b62d0bc07f6b31e1f68e0859c77713e88bf Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Thu, 18 Jan 2024 10:37:26 +0100
Subject: [PATCH 098/227] Compute index only once

---
 subworkflows/local/genome_quant.nf | 27 ++++++---------------
 subworkflows/local/umi_dedup.nf    | 11 ---------
 workflows/smrnaseq.nf              | 39 ++++++++++++++++--------------
 3 files changed, 28 insertions(+), 49 deletions(-)

diff --git a/subworkflows/local/genome_quant.nf b/subworkflows/local/genome_quant.nf
index b310c76a..0f97f16b 100644
--- a/subworkflows/local/genome_quant.nf
+++ b/subworkflows/local/genome_quant.nf
@@ -8,32 +8,19 @@ include { BOWTIE_MAP_SEQ as BOWTIE_MAP_GENOME } from '../../modules/local/bowtie
 
 workflow GENOME_QUANT {
     take:
-    fasta
-    index
+    bowtie_index
+    fasta_formatted // fasta as generated by bowtie index step
     reads // channel: [ val(meta), [ reads ] ]
 
     main:
     ch_versions = Channel.empty()
 
-    if (!index){
-        INDEX_GENOME ( [ [:], fasta ] )
-        bowtie_index    = INDEX_GENOME.out.index
-        fasta_formatted = INDEX_GENOME.out.fasta
-        ch_versions     = ch_versions.mix(INDEX_GENOME.out.versions)
-    } else {
-        bowtie_index    = Channel.fromPath("${index}**ebwt", checkIfExists: true).ifEmpty { exit 1, "Bowtie1 index directory not found: ${index}" }
-        fasta_formatted = fasta
-    }
+    BOWTIE_MAP_GENOME ( reads, bowtie_index.collect() )
+    ch_versions = ch_versions.mix(BOWTIE_MAP_GENOME.out.versions)
 
-    if (bowtie_index){
-        BOWTIE_MAP_GENOME ( reads, bowtie_index.collect() )
-        ch_versions = ch_versions.mix(BOWTIE_MAP_GENOME.out.versions)
-        fasta_formatted
-            .map { file -> tuple(file.baseName, file) }
-            .set { sort_input }
-        BAM_SORT_STATS_SAMTOOLS ( BOWTIE_MAP_GENOME.out.bam,  sort_input )
-        ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions)
-    }
+    ch_fasta_formatted_for_sort = fasta_formatted .map { file -> tuple(file.baseName, file) }
+    BAM_SORT_STATS_SAMTOOLS ( BOWTIE_MAP_GENOME.out.bam,  ch_fasta_formatted_for_sort )
+    ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions)
 
     emit:
     fasta    = fasta_formatted
diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index f8981758..2eda1b60 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -12,7 +12,6 @@ include { CAT_CAT                             } from '../../modules/nf-core/cat/
 
 workflow DEDUPLICATE_UMIS {
     take:
-    fasta
     bt_index
     reads      // channel: [ val(meta), [ reads ] ]
     val_get_dedup_stats //boolean true/false
@@ -22,16 +21,6 @@ workflow DEDUPLICATE_UMIS {
     ch_versions = Channel.empty()
     ch_dedup_stats = Channel.empty()
 
-    if (!bt_index){
-        INDEX_GENOME ( [ [:], fasta ] )
-        bt_index      = INDEX_GENOME.out.index
-        fasta_formatted = INDEX_GENOME.out.fasta
-        ch_versions     = ch_versions.mix(INDEX_GENOME.out.versions)
-    } else {
-        fasta_formatted = fasta
-    }
-
-
     UMI_MAP_GENOME ( reads, bt_index.collect() )
     ch_versions = ch_versions.mix(UMI_MAP_GENOME.out.versions)
 
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 4b81796c..56ab5a05 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -150,29 +150,32 @@ workflow SMRNASEQ {
     )
     ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
 
+    ch_fasta = file(params.fasta)
     reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
 
+    //Prepare bowtie index, unless specified
+    //This needs to be done here as the index is used by both UMI deduplication and GENOME_QUANT
+    if(params.bowtie_index) {
+        ch_bowtie_index  = Channel.fromPath("${index}**ebwt", checkIfExists: true).ifEmpty { error "Bowtie1 index directory not found: ${index}" }
+        ch_fasta_formatted = ch_fasta
+    } else {
+        INDEX_GENOME ( [ [:], ch_fasta ] )
+        ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)
+        ch_bowtie_index = INDEX_GENOME.out.index
+        ch_fasta_formatted = INDEX_GENOME.out.fasta
+    }
+
     //
     // SUBWORKFLOW: Deduplicate UMIs by mapping them to the genome
     //
     if (params.with_umi){
-        if (params.fasta){
-            fasta_ch = file(params.fasta)
-
-            //This needs to be done here as GENOME_QUANT should not run prior to the deduplication of UMIs.
-            INDEX_GENOME ( [ [:], fasta_ch ] )
-
-            ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)
-
-            DEDUPLICATE_UMIS (
-                fasta_ch,
-                INDEX_GENOME.out.index,
-                FASTQ_FASTQC_UMITOOLS_FASTP.out.reads,
-                params.umi_stats
-            )
-            reads_for_mirna = DEDUPLICATE_UMIS.out.reads
-            ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)
-        }
+        DEDUPLICATE_UMIS (
+            ch_bowtie_index,
+            FASTQ_FASTQC_UMITOOLS_FASTP.out.reads,
+            params.umi_stats
+        )
+        reads_for_mirna = DEDUPLICATE_UMIS.out.reads
+        ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)
     }
 
 
@@ -225,7 +228,7 @@ workflow SMRNASEQ {
     //
     genome_stats = Channel.empty()
     if (params.fasta){
-        GENOME_QUANT ( file(params.fasta), params.bowtie_index, MIRNA_QUANT.out.unmapped )
+        GENOME_QUANT ( ch_bowtie_index, ch_fasta_formatted, MIRNA_QUANT.out.unmapped )
         genome_stats = GENOME_QUANT.out.stats
         ch_versions = ch_versions.mix(GENOME_QUANT.out.versions)
 

From f04ef2e5ae0faf05cc87202c9cc2fe4df9a8dd6e Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Thu, 18 Jan 2024 10:47:32 +0100
Subject: [PATCH 099/227] Check that fasta is provided when UMIs are used

---
 workflows/smrnaseq.nf | 11 +++++++----
 1 file changed, 7 insertions(+), 4 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 56ab5a05..0fd20636 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -150,19 +150,22 @@ workflow SMRNASEQ {
     )
     ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
 
-    ch_fasta = file(params.fasta)
+    if(params.with_umi && !params.fasta) {
+        error "Specifying a genome fasta is required for UMI deduplication"
+    }
+    ch_fasta = params.fasta ? file(params.fasta): []
     reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
 
     //Prepare bowtie index, unless specified
     //This needs to be done here as the index is used by both UMI deduplication and GENOME_QUANT
     if(params.bowtie_index) {
         ch_bowtie_index  = Channel.fromPath("${index}**ebwt", checkIfExists: true).ifEmpty { error "Bowtie1 index directory not found: ${index}" }
-        ch_fasta_formatted = ch_fasta
     } else {
         INDEX_GENOME ( [ [:], ch_fasta ] )
         ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)
         ch_bowtie_index = INDEX_GENOME.out.index
-        ch_fasta_formatted = INDEX_GENOME.out.fasta
+        // set to reformatted fasta as generated by `bowtie index`
+        ch_fasta = INDEX_GENOME.out.fasta
     }
 
     //
@@ -228,7 +231,7 @@ workflow SMRNASEQ {
     //
     genome_stats = Channel.empty()
     if (params.fasta){
-        GENOME_QUANT ( ch_bowtie_index, ch_fasta_formatted, MIRNA_QUANT.out.unmapped )
+        GENOME_QUANT ( ch_bowtie_index, ch_fasta, MIRNA_QUANT.out.unmapped )
         genome_stats = GENOME_QUANT.out.stats
         ch_versions = ch_versions.mix(GENOME_QUANT.out.versions)
 

From 41bd0d06decdc62bb208e8ddffea48b29d33bb51 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Thu, 18 Jan 2024 10:50:19 +0100
Subject: [PATCH 100/227] Acually use deduplicated reads

---
 workflows/smrnaseq.nf | 13 ++++++-------
 1 file changed, 6 insertions(+), 7 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 0fd20636..f740ab8d 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -154,7 +154,7 @@ workflow SMRNASEQ {
         error "Specifying a genome fasta is required for UMI deduplication"
     }
     ch_fasta = params.fasta ? file(params.fasta): []
-    reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
+    ch_reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
 
     //Prepare bowtie index, unless specified
     //This needs to be done here as the index is used by both UMI deduplication and GENOME_QUANT
@@ -174,10 +174,10 @@ workflow SMRNASEQ {
     if (params.with_umi){
         DEDUPLICATE_UMIS (
             ch_bowtie_index,
-            FASTQ_FASTQC_UMITOOLS_FASTP.out.reads,
+            ch_reads_for_mirna,
             params.umi_stats
         )
-        reads_for_mirna = DEDUPLICATE_UMIS.out.reads
+        ch_reads_for_mirna = DEDUPLICATE_UMIS.out.reads
         ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)
     }
 
@@ -186,7 +186,7 @@ workflow SMRNASEQ {
     // SUBWORKFLOW: mirtrace QC
     //
     FASTQ_FASTQC_UMITOOLS_FASTP.out.adapter_seq
-    .join( FASTQ_FASTQC_UMITOOLS_FASTP.out.reads )
+    .join( ch_reads_for_mirna )
     .map { meta, adapter_seq, reads -> [adapter_seq, meta.id, reads] }
     .groupTuple()
     .set { ch_mirtrace_inputs }
@@ -199,7 +199,6 @@ workflow SMRNASEQ {
     // SUBWORKFLOW: remove contaminants from reads
     //
     contamination_stats = Channel.empty()
-    mirna_reads = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
     if (params.filter_contamination){
         CONTAMINANT_FILTER (
             reference_hairpin,
@@ -209,7 +208,7 @@ workflow SMRNASEQ {
             params.ncrna,
             params.pirna,
             params.other_contamination,
-            FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
+            ch_reads_for_mirna
         )
 
         contamination_stats = CONTAMINANT_FILTER.out.filter_stats
@@ -240,7 +239,7 @@ workflow SMRNASEQ {
 
         if (!params.skip_mirdeep) {
             MIRDEEP2 (
-                FASTQ_FASTQC_UMITOOLS_FASTP.out.reads,
+                ch_reads_for_mirna,
                 GENOME_QUANT.out.fasta,
                 GENOME_QUANT.out.index.collect(),
                 hairpin_clean,

From 6213f5f06245917975a13f0d07fd19aebba36bee Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Thu, 18 Jan 2024 11:01:49 +0100
Subject: [PATCH 101/227] Use contaminant-filtered reads downstream

---
 modules/local/mirtrace.nf | 2 +-
 workflows/smrnaseq.nf     | 4 ++--
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/local/mirtrace.nf b/modules/local/mirtrace.nf
index 95989293..500de058 100644
--- a/modules/local/mirtrace.nf
+++ b/modules/local/mirtrace.nf
@@ -44,7 +44,7 @@ process MIRTRACE_RUN {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        mirtrace: \$(echo \$(mirtrace -v 2>&1))
+        mirtrace: \$(echo \$(mirtrace -v))
     END_VERSIONS
     """
 
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index f740ab8d..29010308 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -213,7 +213,7 @@ workflow SMRNASEQ {
 
         contamination_stats = CONTAMINANT_FILTER.out.filter_stats
         ch_versions = ch_versions.mix(CONTAMINANT_FILTER.out.versions)
-        mirna_reads = CONTAMINANT_FILTER.out.filtered_reads
+        ch_reads_for_mirna = CONTAMINANT_FILTER.out.filtered_reads
 
     }
 
@@ -221,7 +221,7 @@ workflow SMRNASEQ {
         [ [:], reference_mature],
         [ [:], reference_hairpin],
         mirna_gtf,
-        mirna_reads
+        ch_reads_for_mirna
     )
     ch_versions = ch_versions.mix(MIRNA_QUANT.out.versions.ifEmpty(null))
 

From ed8ad08092229f322c0f1ddb4d6dc515bc5049fe Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Thu, 18 Jan 2024 11:19:13 +0100
Subject: [PATCH 102/227] Ensure fasta is optional

---
 workflows/smrnaseq.nf | 24 ++++++++++++++----------
 1 file changed, 14 insertions(+), 10 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 29010308..7c1103d4 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -156,16 +156,20 @@ workflow SMRNASEQ {
     ch_fasta = params.fasta ? file(params.fasta): []
     ch_reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
 
-    //Prepare bowtie index, unless specified
-    //This needs to be done here as the index is used by both UMI deduplication and GENOME_QUANT
-    if(params.bowtie_index) {
-        ch_bowtie_index  = Channel.fromPath("${index}**ebwt", checkIfExists: true).ifEmpty { error "Bowtie1 index directory not found: ${index}" }
-    } else {
-        INDEX_GENOME ( [ [:], ch_fasta ] )
-        ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)
-        ch_bowtie_index = INDEX_GENOME.out.index
-        // set to reformatted fasta as generated by `bowtie index`
-        ch_fasta = INDEX_GENOME.out.fasta
+    // even if bowtie index is specified, there still needs to be a fasta.
+    // without fasta, no genome analysis.
+    if(params.fasta) {
+        //Prepare bowtie index, unless specified
+        //This needs to be done here as the index is used by both UMI deduplication and GENOME_QUANT
+        if(params.bowtie_index) {
+            ch_bowtie_index  = Channel.fromPath("${index}**ebwt", checkIfExists: true).ifEmpty { error "Bowtie1 index directory not found: ${index}" }
+        } else {
+            INDEX_GENOME ( [ [:], ch_fasta ] )
+            ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)
+            ch_bowtie_index = INDEX_GENOME.out.index
+            // set to reformatted fasta as generated by `bowtie index`
+            ch_fasta = INDEX_GENOME.out.fasta
+        }
     }
 
     //

From ed99b7ea082557c8969117a373d2ba7eb2205502 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Thu, 18 Jan 2024 11:41:23 +0100
Subject: [PATCH 103/227] Attempt to disable umi_stats on CI

---
 conf/test_umi.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/test_umi.config b/conf/test_umi.config
index 53a58f41..7d9e6e53 100644
--- a/conf/test_umi.config
+++ b/conf/test_umi.config
@@ -33,5 +33,6 @@ params {
     umitools_extract_method = 'regex'
     umitools_bc_pattern = '.+AACTGTAGGCACCATCAAT{s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
     save_umi_intermeds = true
+    umi_stats = false // takes too much memory for CI
 
 }

From 1c278fe0e755a102de7d2c299bed02925699a2d2 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Fri, 19 Jan 2024 09:32:03 +0100
Subject: [PATCH 104/227] increase limits for umitools dedup

---
 conf/modules.config | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index 23406e58..b64c6691 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -199,6 +199,8 @@ if (params.with_umi) {
                     )
                 ]
             ]
+            time = { check_max( 120.h , 'time' ) }
+            memory = { check_max( 72.GB * task.attempt, 'memory' ) }
         }
 
         withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' {

From 91e67f2520715b5e12c14e9fb213905b49df85c3 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Mon, 22 Jan 2024 12:28:34 +0000
Subject: [PATCH 105/227] Adding in possibiltiy to use different method for UMI
 grouping

---
 conf/modules.config  | 2 +-
 nextflow.config      | 1 +
 nextflow_schema.json | 7 +++++++
 3 files changed, 9 insertions(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index b64c6691..28790647 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -182,7 +182,7 @@ if (params.with_umi && !params.skip_umi_extract) {
 if (params.with_umi) {
     process {
         withName: '.*:DEDUPLICATE_UMIS:UMITOOLS_DEDUP' {
-            ext.args = { meta.single_end ? '' : '--unpaired-reads=discard --chimeric-pairs=discard' }
+            ext.args = { meta.single_end ? '--method $params.method' : '--method $params.method --unpaired-reads=discard --chimeric-pairs=discard' }
             ext.prefix = { "${meta.id}.umi_dedup.sorted" }
             publishDir = [
                 [
diff --git a/nextflow.config b/nextflow.config
index 4d7eb423..82058ae6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -36,6 +36,7 @@ params {
     umitools_extract_method    = 'string'
     umitools_bc_pattern        = null
     umi_discard_read           = null
+    umitools_method            = 'directional'
     save_umi_intermeds         = false
     umi_merge_unmapped         = true
     umi_stats                  = true
diff --git a/nextflow_schema.json b/nextflow_schema.json
index cd8fa7a5..a5434a37 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -68,6 +68,13 @@
                     "description": "UMI pattern to use. Can be either 'string' (default) or 'regex'.",
                     "help_text": "More details can be found in the [UMI-tools documentation](https://umi-tools.readthedocs.io/en/latest/reference/extract.html#extract-method).\n"
                 },
+                "umitools_method": {
+                    "type": "string",
+                    "default": "directional",
+                    "description": "UMI grouping method",
+                    "fa_icon": "fas fa-layer-group",
+                    "help_text": "Available options are unique, percentile, cluster, adjacency or directional."
+                },
                 "skip_umi_extract": {
                     "type": "boolean",
                     "fa_icon": "fas fa-compress-alt",

From cfac917f8f03e74001bc518aa38041d5d147928f Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Mon, 22 Jan 2024 13:41:22 +0100
Subject: [PATCH 106/227] Install umicollapse instead of umitools dedup

---
 modules.json                                  | 82 ++++++++++++++-----
 .../dedup => umicollapse}/environment.yml     |  4 +-
 modules/nf-core/umicollapse/main.nf           | 50 +++++++++++
 modules/nf-core/umicollapse/meta.yml          | 51 ++++++++++++
 modules/nf-core/umitools/dedup/main.nf        | 62 --------------
 modules/nf-core/umitools/dedup/meta.yml       | 71 ----------------
 6 files changed, 165 insertions(+), 155 deletions(-)
 rename modules/nf-core/{umitools/dedup => umicollapse}/environment.yml (56%)
 create mode 100644 modules/nf-core/umicollapse/main.nf
 create mode 100644 modules/nf-core/umicollapse/meta.yml
 delete mode 100644 modules/nf-core/umitools/dedup/main.nf
 delete mode 100644 modules/nf-core/umitools/dedup/meta.yml

diff --git a/modules.json b/modules.json
index 9d68299f..53f34207 100644
--- a/modules.json
+++ b/modules.json
@@ -8,72 +8,107 @@
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "81f27e75847087865299cc46605deb3b09b4e0a2",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "02fd5bd7275abad27aad32d5c852e0a9b1b98882",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "1799e452de650f6fb8890d25829bca23014b0728",
-                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
+                        "installed_by": [
+                            "fastq_fastqc_umitools_fastp",
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "617777a807a1770f73deb38c80004bac06807eef",
-                        "installed_by": ["fastq_fastqc_umitools_fastp"]
+                        "installed_by": [
+                            "fastq_fastqc_umitools_fastp"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/bam2fq": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": ["bam_stats_samtools", "modules"]
+                        "installed_by": [
+                            "bam_stats_samtools",
+                            "modules"
+                        ]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": ["bam_stats_samtools", "modules"]
+                        "installed_by": [
+                            "bam_stats_samtools",
+                            "modules"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": ["bam_sort_stats_samtools", "modules"]
+                        "installed_by": [
+                            "bam_sort_stats_samtools",
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": ["bam_sort_stats_samtools", "modules"]
+                        "installed_by": [
+                            "bam_sort_stats_samtools",
+                            "modules"
+                        ]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": ["bam_stats_samtools", "modules"]
+                        "installed_by": [
+                            "bam_stats_samtools",
+                            "modules"
+                        ]
                     },
-                    "umitools/dedup": {
+                    "umicollapse": {
                         "branch": "master",
-                        "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53",
-                        "installed_by": ["modules"]
+                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "umitools/extract": {
                         "branch": "master",
                         "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53",
-                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
+                        "installed_by": [
+                            "fastq_fastqc_umitools_fastp",
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -82,20 +117,27 @@
                     "bam_sort_stats_samtools": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": ["bam_sort_stats_samtools", "subworkflows"]
+                        "installed_by": [
+                            "bam_sort_stats_samtools",
+                            "subworkflows"
+                        ]
                     },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
                         "git_sha": "d20574ce7420a54fe47b46a91c5f2215d1a2777c",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/umitools/dedup/environment.yml b/modules/nf-core/umicollapse/environment.yml
similarity index 56%
rename from modules/nf-core/umitools/dedup/environment.yml
rename to modules/nf-core/umicollapse/environment.yml
index f443735f..8dbc65dc 100644
--- a/modules/nf-core/umitools/dedup/environment.yml
+++ b/modules/nf-core/umicollapse/environment.yml
@@ -1,7 +1,7 @@
-name: umitools_dedup
+name: umicollapse
 channels:
   - conda-forge
   - bioconda
   - defaults
 dependencies:
-  - bioconda::umi_tools=1.1.4
+  - bioconda::umicollapse=1.0.0
diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
new file mode 100644
index 00000000..01ad2541
--- /dev/null
+++ b/modules/nf-core/umicollapse/main.nf
@@ -0,0 +1,50 @@
+process UMICOLLAPSE {
+    tag "$meta.id"
+    label "process_high_memory"
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/umicollapse:1.0.0--hdfd78af_1' :
+        'biocontainers/umicollapse:1.0.0--hdfd78af_1' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+
+    output:
+    tuple val(meta), path("*.bam"), emit: bam
+    tuple val(meta), path("*.log"), emit: log
+    path  "versions.yml"          , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args   = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+
+    """
+    umicollapse \\
+        bam \\
+        -i $bam \\
+        -o ${prefix}.bam \\
+        $args
+
+    mv .command.log ${prefix}_UMICollapse.log
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        umicollapse: $VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.dedup.bam
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        umicollapse: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/umicollapse/meta.yml b/modules/nf-core/umicollapse/meta.yml
new file mode 100644
index 00000000..528bc0ee
--- /dev/null
+++ b/modules/nf-core/umicollapse/meta.yml
@@ -0,0 +1,51 @@
+---
+name: "umicollapse"
+description: Deduplicate reads based on the mapping co-ordinate and the UMI attached to the read.
+keywords:
+  - umicollapse
+  - deduplication
+  - genomics
+tools:
+  - "umicollapse":
+      description: "UMICollapse contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs)."
+      homepage: "https://github.com/Daniel-Liu-c0deb0t/UMICollapse"
+      documentation: "https://github.com/Daniel-Liu-c0deb0t/UMICollapse"
+      tool_dev_url: "https://github.com/Daniel-Liu-c0deb0t/UMICollapse"
+      doi: "10.7717/peerj.8275"
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: |
+        BAM file containing reads to be deduplicated via UMIs.
+      pattern: "*.{bam}"
+  - bai:
+      type: file
+      description: |
+        BAM index files corresponding to the input BAM file.
+      pattern: "*.{bai}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM file with deduplicated UMIs.
+      pattern: "*.{bam}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@CharlotteAnne"
+  - "@chris-cheshire"
+maintainers:
+  - "@CharlotteAnne"
+  - "@chris-cheshire"
diff --git a/modules/nf-core/umitools/dedup/main.nf b/modules/nf-core/umitools/dedup/main.nf
deleted file mode 100644
index 64ab8f98..00000000
--- a/modules/nf-core/umitools/dedup/main.nf
+++ /dev/null
@@ -1,62 +0,0 @@
-process UMITOOLS_DEDUP {
-    tag "$meta.id"
-    label "process_medium"
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.4--py38hbff2b2d_1' :
-        'biocontainers/umi_tools:1.1.4--py38hbff2b2d_1' }"
-
-    input:
-    tuple val(meta), path(bam), path(bai)
-    val get_output_stats
-
-    output:
-    tuple val(meta), path("${prefix}.bam")     , emit: bam
-    tuple val(meta), path("*.log")             , emit: log
-    tuple val(meta), path("*edit_distance.tsv"), optional:true, emit: tsv_edit_distance
-    tuple val(meta), path("*per_umi.tsv")      , optional:true, emit: tsv_per_umi
-    tuple val(meta), path("*per_position.tsv") , optional:true, emit: tsv_umi_per_position
-    path  "versions.yml"                       , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    def paired = meta.single_end ? "" : "--paired"
-    stats = get_output_stats ? "--output-stats ${prefix}" : ""
-    if ("$bam" == "${prefix}.bam") error "Input and output names are the same, set prefix in module configuration to disambiguate!"
-
-    if (!(args ==~ /.*--random-seed.*/)) {args += " --random-seed=100"}
-    """
-    PYTHONHASHSEED=0 umi_tools \\
-        dedup \\
-        -I $bam \\
-        -S ${prefix}.bam \\
-        -L ${prefix}.log \\
-        $stats \\
-        $paired \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' )
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    touch ${prefix}.bam
-    touch ${prefix}.log
-    touch ${prefix}_edit_distance.tsv
-    touch ${prefix}_per_umi.tsv
-    touch ${prefix}_per_position.tsv
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/umitools/dedup/meta.yml b/modules/nf-core/umitools/dedup/meta.yml
deleted file mode 100644
index 38d3fd46..00000000
--- a/modules/nf-core/umitools/dedup/meta.yml
+++ /dev/null
@@ -1,71 +0,0 @@
-name: umitools_dedup
-description: Deduplicate reads based on the mapping co-ordinate and the UMI attached to the read.
-keywords:
-  - umitools
-  - deduplication
-  - dedup
-tools:
-  - umi_tools:
-      description: >
-        UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes
-
-      documentation: https://umi-tools.readthedocs.io/en/latest/
-      license: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: |
-        BAM file containing reads to be deduplicated via UMIs.
-      pattern: "*.{bam}"
-  - bai:
-      type: file
-      description: |
-        BAM index files corresponding to the input BAM file.
-      pattern: "*.{bai}"
-  - get_output_stats:
-      type: boolean
-      description: |
-        Whether or not to generate output stats.
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM file with deduplicated UMIs.
-      pattern: "*.{bam}"
-  - log:
-      type: file
-      description: File with logging information
-      pattern: "*.{log}"
-  - tsv_edit_distance:
-      type: file
-      description: Reports the (binned) average edit distance between the UMIs at each position.
-      pattern: "*edit_distance.tsv"
-  - tsv_per_umi:
-      type: file
-      description: UMI-level summary statistics.
-      pattern: "*per_umi.tsv"
-  - tsv_umi_per_position:
-      type: file
-      description: Tabulates the counts for unique combinations of UMI and position.
-      pattern: "*per_position.tsv"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@drpatelh"
-  - "@grst"
-  - "@klkeys"
-maintainers:
-  - "@drpatelh"
-  - "@grst"
-  - "@klkeys"

From b9208eace2d4c052a9496084002057878cd504c2 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Mon, 22 Jan 2024 13:55:37 +0100
Subject: [PATCH 107/227] Switch to umicollapse

---
 conf/modules.config             | 23 ++++++++---------------
 nextflow.config                 |  3 +--
 nextflow_schema.json            |  9 ++-------
 subworkflows/local/umi_dedup.nf | 11 ++++-------
 4 files changed, 15 insertions(+), 31 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 28790647..9fe19d49 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -181,23 +181,16 @@ if (params.with_umi && !params.skip_umi_extract) {
 
 if (params.with_umi) {
     process {
-        withName: '.*:DEDUPLICATE_UMIS:UMITOOLS_DEDUP' {
-            ext.args = { meta.single_end ? '--method $params.method' : '--method $params.method --unpaired-reads=discard --chimeric-pairs=discard' }
+        withName: '.*:DEDUPLICATE_UMIS:UMICOLLAPSE' {
+            ext.args = { meta.single_end ? '--algo ${params.umitools_method}' : '--method ${params.umitools_method} --paired --remove-unpaired --remove-chimeric' }
             ext.prefix = { "${meta.id}.umi_dedup.sorted" }
             publishDir = [
-                [
-                    path: { "${params.outdir}/umi_dedup/umitools" },
-                    mode: params.publish_dir_mode,
-                    pattern: '*.tsv'
-                ],
-                [
-                    path: { "${params.outdir}/umi_dedup" },
-                    mode: params.publish_dir_mode,
-                    pattern: '*.bam',
-                    enabled: (
-                        params.save_umi_intermeds
-                    )
-                ]
+                path: { "${params.outdir}/umi_dedup" },
+                mode: params.publish_dir_mode,
+                pattern: '*.bam',
+                enabled: (
+                    params.save_umi_intermeds
+                )
             ]
             time = { check_max( 120.h , 'time' ) }
             memory = { check_max( 72.GB * task.attempt, 'memory' ) }
diff --git a/nextflow.config b/nextflow.config
index 82058ae6..0c3d8b45 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -36,10 +36,9 @@ params {
     umitools_extract_method    = 'string'
     umitools_bc_pattern        = null
     umi_discard_read           = null
-    umitools_method            = 'directional'
+    umitools_method            = 'dir'
     save_umi_intermeds         = false
     umi_merge_unmapped         = true
-    umi_stats                  = true
 
     // Trimming options
     clip_r1                     = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index a5434a37..c4d62d96 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -70,10 +70,10 @@
                 },
                 "umitools_method": {
                     "type": "string",
-                    "default": "directional",
+                    "default": "dir",
                     "description": "UMI grouping method",
                     "fa_icon": "fas fa-layer-group",
-                    "help_text": "Available options are unique, percentile, cluster, adjacency or directional."
+                    "help_text": "Available options are dir, cc, adj"
                 },
                 "skip_umi_extract": {
                     "type": "boolean",
@@ -101,11 +101,6 @@
                     "fa_icon": "fas fa-save",
                     "description": "Unless deactivated the deduplicated reads are merged with the reads that could not be placed to reduce the potential reference bias",
                     "default": true
-                },
-                "umi_stats": {
-                    "type": "boolean",
-                    "default": true,
-                    "description": "Compute UMI statistics for MultiQC"
                 }
             },
             "fa_icon": "fas fa-barcode"
diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 2eda1b60..01fe678c 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -5,7 +5,7 @@
 include { INDEX_GENOME                        } from '../../modules/local/bowtie_genome'
 include { BOWTIE_MAP_SEQ as UMI_MAP_GENOME    } from '../../modules/local/bowtie_map_mirna'
 include { BAM_SORT_STATS_SAMTOOLS             } from '../../subworkflows/nf-core/bam_sort_stats_samtools'
-include { UMITOOLS_DEDUP                      } from '../../modules/nf-core/umitools/dedup/main'
+include { UMICOLLAPSE                         } from '../../modules/nf-core/umicollapse/main'
 include { SAMTOOLS_BAM2FQ                     } from '../../modules/nf-core/samtools/bam2fq/main'
 include { CAT_CAT                             } from '../../modules/nf-core/cat/cat/main'
 
@@ -19,7 +19,6 @@ workflow DEDUPLICATE_UMIS {
     main:
 
     ch_versions = Channel.empty()
-    ch_dedup_stats = Channel.empty()
 
     UMI_MAP_GENOME ( reads, bt_index.collect() )
     ch_versions = ch_versions.mix(UMI_MAP_GENOME.out.versions)
@@ -28,11 +27,10 @@ workflow DEDUPLICATE_UMIS {
     ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions)
 
     ch_umi_dedup = BAM_SORT_STATS_SAMTOOLS.out.bam.join(BAM_SORT_STATS_SAMTOOLS.out.bai)
-    UMITOOLS_DEDUP ( ch_umi_dedup, val_get_dedup_stats)
-    ch_versions = ch_versions.mix(UMITOOLS_DEDUP.out.versions)
-    ch_dedup_stats = ch_dedup_stats.mix(UMITOOLS_DEDUP.out.tsv_edit_distance).join(UMITOOLS_DEDUP.out.tsv_per_umi).join(UMITOOLS_DEDUP.out.tsv_umi_per_position)
+    UMICOLLAPSE ( ch_umi_dedup, val_get_dedup_stats)
+    ch_versions = ch_versions.mix(UMICOLLAPSE.out.versions)
 
-    SAMTOOLS_BAM2FQ ( UMITOOLS_DEDUP.out.bam, false )
+    SAMTOOLS_BAM2FQ ( UMICOLLAPSE.out.bam, false )
     ch_versions = ch_versions.mix(SAMTOOLS_BAM2FQ.out.versions)
 
     ch_dedup_reads = SAMTOOLS_BAM2FQ.out.reads
@@ -52,6 +50,5 @@ workflow DEDUPLICATE_UMIS {
     emit:
     reads    = ch_dedup_reads
     indices  = bt_index
-    stats    = ch_dedup_stats
     versions = ch_versions
 }

From 65f7f0e0cc812c9921129a0200ee457189fa75d2 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Mon, 22 Jan 2024 14:15:05 +0100
Subject: [PATCH 108/227] Switch to umicollapse

---
 conf/modules.config             | 4 +---
 conf/test_umi.config            | 2 --
 subworkflows/local/umi_dedup.nf | 3 +--
 workflows/smrnaseq.nf           | 1 -
 4 files changed, 2 insertions(+), 8 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 9fe19d49..a5f7ea37 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -182,7 +182,7 @@ if (params.with_umi && !params.skip_umi_extract) {
 if (params.with_umi) {
     process {
         withName: '.*:DEDUPLICATE_UMIS:UMICOLLAPSE' {
-            ext.args = { meta.single_end ? '--algo ${params.umitools_method}' : '--method ${params.umitools_method} --paired --remove-unpaired --remove-chimeric' }
+            ext.args = { meta.single_end ? "--algo ${params.umitools_method}" : "--method ${params.umitools_method} --paired --remove-unpaired --remove-chimeric" }
             ext.prefix = { "${meta.id}.umi_dedup.sorted" }
             publishDir = [
                 path: { "${params.outdir}/umi_dedup" },
@@ -192,8 +192,6 @@ if (params.with_umi) {
                     params.save_umi_intermeds
                 )
             ]
-            time = { check_max( 120.h , 'time' ) }
-            memory = { check_max( 72.GB * task.attempt, 'memory' ) }
         }
 
         withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' {
diff --git a/conf/test_umi.config b/conf/test_umi.config
index 7d9e6e53..48f61181 100644
--- a/conf/test_umi.config
+++ b/conf/test_umi.config
@@ -33,6 +33,4 @@ params {
     umitools_extract_method = 'regex'
     umitools_bc_pattern = '.+AACTGTAGGCACCATCAAT{s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
     save_umi_intermeds = true
-    umi_stats = false // takes too much memory for CI
-
 }
diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 01fe678c..5ef4b908 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -14,7 +14,6 @@ workflow DEDUPLICATE_UMIS {
     take:
     bt_index
     reads      // channel: [ val(meta), [ reads ] ]
-    val_get_dedup_stats //boolean true/false
 
     main:
 
@@ -27,7 +26,7 @@ workflow DEDUPLICATE_UMIS {
     ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions)
 
     ch_umi_dedup = BAM_SORT_STATS_SAMTOOLS.out.bam.join(BAM_SORT_STATS_SAMTOOLS.out.bai)
-    UMICOLLAPSE ( ch_umi_dedup, val_get_dedup_stats)
+    UMICOLLAPSE(ch_umi_dedup)
     ch_versions = ch_versions.mix(UMICOLLAPSE.out.versions)
 
     SAMTOOLS_BAM2FQ ( UMICOLLAPSE.out.bam, false )
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 7c1103d4..58a4e8ad 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -179,7 +179,6 @@ workflow SMRNASEQ {
         DEDUPLICATE_UMIS (
             ch_bowtie_index,
             ch_reads_for_mirna,
-            params.umi_stats
         )
         ch_reads_for_mirna = DEDUPLICATE_UMIS.out.reads
         ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)

From 82436515987080d4cf132cfbc1b31c7c2ade9d8f Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Mon, 22 Jan 2024 13:16:26 +0000
Subject: [PATCH 109/227] [automated] Fix linting with Prettier

---
 modules.json | 78 ++++++++++++----------------------------------------
 1 file changed, 18 insertions(+), 60 deletions(-)

diff --git a/modules.json b/modules.json
index 53f34207..03697a05 100644
--- a/modules.json
+++ b/modules.json
@@ -8,107 +8,72 @@
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "81f27e75847087865299cc46605deb3b09b4e0a2",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "02fd5bd7275abad27aad32d5c852e0a9b1b98882",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "1799e452de650f6fb8890d25829bca23014b0728",
-                        "installed_by": [
-                            "fastq_fastqc_umitools_fastp",
-                            "modules"
-                        ]
+                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "617777a807a1770f73deb38c80004bac06807eef",
-                        "installed_by": [
-                            "fastq_fastqc_umitools_fastp"
-                        ]
+                        "installed_by": ["fastq_fastqc_umitools_fastp"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/bam2fq": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": [
-                            "bam_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": [
-                            "bam_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": [
-                            "bam_sort_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": [
-                            "bam_sort_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": [
-                            "bam_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "umicollapse": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "umitools/extract": {
                         "branch": "master",
                         "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53",
-                        "installed_by": [
-                            "fastq_fastqc_umitools_fastp",
-                            "modules"
-                        ]
+                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     }
                 }
             },
@@ -117,27 +82,20 @@
                     "bam_sort_stats_samtools": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": [
-                            "bam_sort_stats_samtools",
-                            "subworkflows"
-                        ]
+                        "installed_by": ["bam_sort_stats_samtools", "subworkflows"]
                     },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
                         "git_sha": "d20574ce7420a54fe47b46a91c5f2215d1a2777c",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}

From ea68c8e6864ecfffeee684915ecc93fca2cd184c Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Mon, 22 Jan 2024 15:03:13 +0100
Subject: [PATCH 110/227] Don't merge deduplicated fastqs with unmapped fastqs

---
 CHANGELOG.md                    |  3 ++-
 docs/output.md                  |  2 +-
 nextflow.config                 |  1 -
 nextflow_schema.json            |  6 ------
 subworkflows/local/umi_dedup.nf | 12 ------------
 5 files changed, 3 insertions(+), 21 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index fc286f02..10fc5720 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -16,10 +16,11 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | ------------- | --------------------------- |
 |               | `--with_umi`                |
 |               | `--umitools_extract_method` |
+|               | `--umitools_method`         |
+|               | `--skip_umi_extract`        |
 |               | `--umitools_bc_pattern`     |
 |               | `--umi_discard_read`        |
 |               | `--save_umi_intermeds`      |
-|               | `--umi_merge_unmapped`      |
 
 ### Software dependencies
 
diff --git a/docs/output.md b/docs/output.md
index fc9d14ef..537f17c5 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -83,7 +83,7 @@ FastP can automatically detect adapter sequences when not specified directly by
   - `samtools_stats/` - `*.{stats,flagstat,idxstats}:` Statistics on the mappings underlying the UMI deduplication.
   </details>
 
-[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name as highlighted in the [UMI-tools extract](#umi-tools-extract) section. The reads are deduplicated based on an alignment against the full genome of the species. The deduplicated reads are then converted into fastq format and merged with the reads that remained unmapped in order to reduce potential reference bias. This behavior can be stopped by setting `--umi_merge_unmapped false`. The resulting fastq files are used in the remaining steps of the pipeline.
+[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name as highlighted in the [UMI-tools extract](#umi-tools-extract) section. The reads are deduplicated based on an alignment against the full genome of the species. The deduplicated reads are then converted into fastq format. The resulting fastq files are used in the remaining steps of the pipeline.
 
 ## Bowtie2
 
diff --git a/nextflow.config b/nextflow.config
index 0c3d8b45..0ea71080 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -38,7 +38,6 @@ params {
     umi_discard_read           = null
     umitools_method            = 'dir'
     save_umi_intermeds         = false
-    umi_merge_unmapped         = true
 
     // Trimming options
     clip_r1                     = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index c4d62d96..204376ab 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -95,12 +95,6 @@
                     "type": "boolean",
                     "fa_icon": "fas fa-save",
                     "description": "If this option is specified, intermediate FastQ and BAM files produced by UMI-tools are also saved in the results directory."
-                },
-                "umi_merge_unmapped": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-save",
-                    "description": "Unless deactivated the deduplicated reads are merged with the reads that could not be placed to reduce the potential reference bias",
-                    "default": true
                 }
             },
             "fa_icon": "fas fa-barcode"
diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 5ef4b908..9f65fa8e 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -34,18 +34,6 @@ workflow DEDUPLICATE_UMIS {
 
     ch_dedup_reads = SAMTOOLS_BAM2FQ.out.reads
 
-    if ( params.umi_merge_unmapped ) {
-
-        SAMTOOLS_BAM2FQ.out.reads
-            .join(UMI_MAP_GENOME.out.unmapped)
-            .map { meta, file1, file2 -> [meta, [file1, file2]]}
-            .set { ch_cat }
-
-        CAT_CAT ( ch_cat )
-        ch_dedup_reads = CAT_CAT.out.file_out
-    }
-
-
     emit:
     reads    = ch_dedup_reads
     indices  = bt_index

From d493ff7b51adb5184c80d0e89cfce2d5482aa0bb Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Mon, 22 Jan 2024 16:07:39 +0100
Subject: [PATCH 111/227] Try to set heap size

---
 modules/nf-core/umicollapse/main.nf | 1 +
 1 file changed, 1 insertion(+)

diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 01ad2541..593463f8 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -25,6 +25,7 @@ process UMICOLLAPSE {
 
     """
     umicollapse \\
+        -Xmx${task.memory.toMega() * 0.8}M \\
         bam \\
         -i $bam \\
         -o ${prefix}.bam \\

From 6fe662941fa3edfa409d98f40e3041a6d22c6392 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Mon, 22 Jan 2024 16:13:20 +0100
Subject: [PATCH 112/227] cast heap size to int

---
 modules/nf-core/umicollapse/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 593463f8..314e0df1 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -25,7 +25,7 @@ process UMICOLLAPSE {
 
     """
     umicollapse \\
-        -Xmx${task.memory.toMega() * 0.8}M \\
+        -Xmx${(task.memory.toMega() * 0.8).intValue()}M \\
         bam \\
         -i $bam \\
         -o ${prefix}.bam \\

From 58c5b1a5c78c5618d6a7e1d5b4fe3f557c162c9c Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Tue, 23 Jan 2024 09:19:42 +0100
Subject: [PATCH 113/227] Increase java stack size

---
 modules/nf-core/umicollapse/main.nf | 1 +
 1 file changed, 1 insertion(+)

diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 314e0df1..fdecd6d9 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -26,6 +26,7 @@ process UMICOLLAPSE {
     """
     umicollapse \\
         -Xmx${(task.memory.toMega() * 0.8).intValue()}M \\
+        -Xss99M \\
         bam \\
         -i $bam \\
         -o ${prefix}.bam \\

From 53c6ffa861eb9c8894e3859d0fb1ddf673eb9fd2 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Tue, 23 Jan 2024 09:37:39 +0100
Subject: [PATCH 114/227] Set jvm options via JAVA_TOOL_OPTIONS

---
 modules/nf-core/umicollapse/main.nf | 5 ++---
 1 file changed, 2 insertions(+), 3 deletions(-)

diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index fdecd6d9..90224df0 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -24,9 +24,8 @@ process UMICOLLAPSE {
     def VERSION = '1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
 
     """
-    umicollapse \\
-        -Xmx${(task.memory.toMega() * 0.8).intValue()}M \\
-        -Xss99M \\
+    JAVA_TOOL_OPTIONS="-Xmx${(task.memory.toMega() * 0.8).intValue()}M -Xss99M" \\
+      umicollapse \\
         bam \\
         -i $bam \\
         -o ${prefix}.bam \\

From d5192d48a3491504b41fe1af51860d1d9773c632 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Tue, 23 Jan 2024 09:52:51 +0100
Subject: [PATCH 115/227] Full manual mode

---
 modules/nf-core/umicollapse/main.nf | 16 +++++++++-------
 1 file changed, 9 insertions(+), 7 deletions(-)

diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 90224df0..788f4d1e 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -21,15 +21,17 @@ process UMICOLLAPSE {
     script:
     def args   = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def VERSION = '1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def VERSION = '1.0.0-1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
 
     """
-    JAVA_TOOL_OPTIONS="-Xmx${(task.memory.toMega() * 0.8).intValue()}M -Xss99M" \\
-      umicollapse \\
-        bam \\
-        -i $bam \\
-        -o ${prefix}.bam \\
-        $args
+    java \\
+      -Xmx${(task.memory.toMega() * 0.8).intValue()}M \\
+      -Xss99M \\
+      -jar /usr/local/share/umicollapse-${VERSION}/umicollapse.jar \\
+      bam \\
+      -i $bam \\
+      -o ${prefix}.bam \\
+      $args
 
     mv .command.log ${prefix}_UMICollapse.log
 

From 3d8219ead8e53ee0c5ec8b1895defaaca119d1a8 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Tue, 23 Jan 2024 12:58:11 +0100
Subject: [PATCH 116/227] Use two-pass mode for umicollapse

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index a5f7ea37..127e0a34 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -182,7 +182,7 @@ if (params.with_umi && !params.skip_umi_extract) {
 if (params.with_umi) {
     process {
         withName: '.*:DEDUPLICATE_UMIS:UMICOLLAPSE' {
-            ext.args = { meta.single_end ? "--algo ${params.umitools_method}" : "--method ${params.umitools_method} --paired --remove-unpaired --remove-chimeric" }
+            ext.args = { meta.single_end ? "--algo ${params.umitools_method} --two-pass" : "--method ${params.umitools_method} --two-pass --paired --remove-unpaired --remove-chimeric" }
             ext.prefix = { "${meta.id}.umi_dedup.sorted" }
             publishDir = [
                 path: { "${params.outdir}/umi_dedup" },

From 4fb25232db7bed454daac683b0b3dcb77daa4d1f Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Tue, 23 Jan 2024 13:24:39 +0100
Subject: [PATCH 117/227] Increase stack size even further

---
 modules/nf-core/umicollapse/main.nf | 5 +++--
 1 file changed, 3 insertions(+), 2 deletions(-)

diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 788f4d1e..0ae78118 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -23,10 +23,11 @@ process UMICOLLAPSE {
     def prefix = task.ext.prefix ?: "${meta.id}"
     def VERSION = '1.0.0-1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
 
+    // Things I tried...
     """
     java \\
-      -Xmx${(task.memory.toMega() * 0.8).intValue()}M \\
-      -Xss99M \\
+      -Xmx${(task.memory.toGiga() - 1).intValue()}G \\
+      -Xss1G \\
       -jar /usr/local/share/umicollapse-${VERSION}/umicollapse.jar \\
       bam \\
       -i $bam \\

From 2dd1e24ff2c1cc643e71b5e6a3cfdf3dbd47bd33 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 24 Jan 2024 15:24:55 +0000
Subject: [PATCH 118/227] Updated umicollapse to modules provided version :)

---
 modules.json                                  |   2 +-
 modules/nf-core/umicollapse/main.nf           |  31 ++--
 modules/nf-core/umicollapse/meta.yml          |   4 +
 .../nf-core/umicollapse/tests/main.nf.test    | 153 ++++++++++++++++++
 .../umicollapse/tests/main.nf.test.snap       |  36 +++++
 .../nf-core/umicollapse/tests/nextflow.config |   8 +
 .../umicollapse/tests/nextflow_PE.config      |  10 ++
 .../umicollapse/tests/nextflow_SE.config      |  10 ++
 modules/nf-core/umicollapse/tests/tags.yml    |   2 +
 9 files changed, 243 insertions(+), 13 deletions(-)
 create mode 100644 modules/nf-core/umicollapse/tests/main.nf.test
 create mode 100644 modules/nf-core/umicollapse/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/umicollapse/tests/nextflow.config
 create mode 100644 modules/nf-core/umicollapse/tests/nextflow_PE.config
 create mode 100644 modules/nf-core/umicollapse/tests/nextflow_SE.config
 create mode 100644 modules/nf-core/umicollapse/tests/tags.yml

diff --git a/modules.json b/modules.json
index 03697a05..8236fcfd 100644
--- a/modules.json
+++ b/modules.json
@@ -67,7 +67,7 @@
                     },
                     "umicollapse": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "b71a681e9ec41cefd330e12b8566b5f5aff4941c",
                         "installed_by": ["modules"]
                     },
                     "umitools/extract": {
diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 0ae78118..291d3273 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -1,5 +1,6 @@
 process UMICOLLAPSE {
     tag "$meta.id"
+    label "process_high"
     label "process_high_memory"
 
     conda "${moduleDir}/environment.yml"
@@ -12,7 +13,7 @@ process UMICOLLAPSE {
 
     output:
     tuple val(meta), path("*.bam"), emit: bam
-    tuple val(meta), path("*.log"), emit: log
+    tuple val(meta), path("*_UMICollapse.log"), emit: log
     path  "versions.yml"          , emit: versions
 
     when:
@@ -22,19 +23,25 @@ process UMICOLLAPSE {
     def args   = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def VERSION = '1.0.0-1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-
-    // Things I tried...
+    // Memory allocation: We need to make sure that both heap and stack size is sufficiently large for
+    // umicollapse. We set the stack size to 5% of the available memory, the heap size to 90%
+    // which leaves 5% for stuff happening outside of java without the scheduler killing the process.
+    def max_heap_size_mega = (task.memory.toMega() * 0.9).intValue()
+    def max_stack_size_mega = (task.memory.toMega() * 0.05).intValue()
     """
+    # Getting the umicollapse jar file like this because `umicollapse` is a Python wrapper script generated
+    # by conda that allows to set the heap size (Xmx), but not the stack size (Xss).
+    # `which` allows us to get the directory that contains `umicollapse`, independent of whether we
+    # are in a container or conda environment.
+    UMICOLLAPSE_JAR=\$(dirname \$(which umicollapse))/../share/umicollapse-${VERSION}/umicollapse.jar
     java \\
-      -Xmx${(task.memory.toGiga() - 1).intValue()}G \\
-      -Xss1G \\
-      -jar /usr/local/share/umicollapse-${VERSION}/umicollapse.jar \\
-      bam \\
-      -i $bam \\
-      -o ${prefix}.bam \\
-      $args
-
-    mv .command.log ${prefix}_UMICollapse.log
+        -Xmx${max_heap_size_mega}M \\
+        -Xss${max_stack_size_mega}M \\
+        -jar \$UMICOLLAPSE_JAR \\
+        bam \\
+        -i $bam \\
+        -o ${prefix}.bam \\
+        $args | tee ${prefix}_UMICollapse.log
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/umicollapse/meta.yml b/modules/nf-core/umicollapse/meta.yml
index 528bc0ee..c45d6932 100644
--- a/modules/nf-core/umicollapse/meta.yml
+++ b/modules/nf-core/umicollapse/meta.yml
@@ -39,6 +39,10 @@ output:
       type: file
       description: BAM file with deduplicated UMIs.
       pattern: "*.{bam}"
+  - log:
+      type: file
+      description: A log file with the deduplication statistics.
+      pattern: "*_{UMICollapse.log}"
   - versions:
       type: file
       description: File containing software versions
diff --git a/modules/nf-core/umicollapse/tests/main.nf.test b/modules/nf-core/umicollapse/tests/main.nf.test
new file mode 100644
index 00000000..1b8bf7e9
--- /dev/null
+++ b/modules/nf-core/umicollapse/tests/main.nf.test
@@ -0,0 +1,153 @@
+nextflow_process {
+
+    name "Test Process UMICOLLAPSE"
+    script "../main.nf"
+    process "UMICOLLAPSE"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "umicollapse"
+    tag "umitools/extract"
+    tag "samtools/index"
+    tag "bwa/index"
+    tag "bwa/mem"
+
+    test("umicollapse single end test") {
+        setup{
+            run("UMITOOLS_EXTRACT"){
+                script "../../umitools/extract/main.nf"
+                config "./nextflow_SE.config"
+                process{
+                    """
+                    input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    [
+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                    ]
+                    ]
+                    """
+                }
+            }
+
+            run("BWA_INDEX"){
+                script "../../bwa/index/main.nf"
+                process{
+                    """
+                    input[0] = [
+                            [ id:'sarscov2'],
+                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                            ]
+                    """
+                }
+            }
+            run("BWA_MEM"){
+                script "../../bwa/mem/main.nf"
+                process{
+                    """
+                    input[0] = UMITOOLS_EXTRACT.out.reads
+                    input[1] = BWA_INDEX.out.index
+                    input[2] = true
+                    """
+                }
+            }
+            run("SAMTOOLS_INDEX"){
+                script "../../samtools/index/main.nf"
+                process{
+                    """
+                    input[0] = BWA_MEM.out.bam
+                    """
+                }
+            }
+        }
+
+        when {
+            config "./nextflow_SE.config"
+            process {
+                """
+                input[0] =  BWA_MEM.out.bam.join(SAMTOOLS_INDEX.out.bai, by: [0])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.bam,
+                    process.out.versions).match() }
+            )
+        }
+
+    }
+
+    test("umicollapse paired tests") {
+        setup{
+            run("UMITOOLS_EXTRACT"){
+                script "../../umitools/extract/main.nf"
+                config "./nextflow_PE.config"
+                process{
+                    """
+                    input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                    ]
+                    ]
+                    """
+                }
+            }
+
+            run("BWA_INDEX"){
+                script "../../bwa/index/main.nf"
+                process{
+                    """
+                    input[0] = [
+                            [ id:'sarscov2'],
+                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                            ]
+                    """
+                }
+            }
+            run("BWA_MEM"){
+                script "../../bwa/mem/main.nf"
+                process{
+                    """
+                    input[0] = UMITOOLS_EXTRACT.out.reads
+                    input[1] = BWA_INDEX.out.index
+                    input[2] = true
+                    """
+                }
+            }
+            run("SAMTOOLS_INDEX"){
+                script "../../samtools/index/main.nf"
+                process{
+                    """
+                    input[0] = BWA_MEM.out.bam
+                    """
+                }
+            }
+        }
+
+        when {
+            config "./nextflow_PE.config"
+            process {
+                """
+                input[0] =  BWA_MEM.out.bam.join(SAMTOOLS_INDEX.out.bai, by: [0])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.bam,
+                    process.out.versions).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/umicollapse/tests/main.nf.test.snap b/modules/nf-core/umicollapse/tests/main.nf.test.snap
new file mode 100644
index 00000000..60250530
--- /dev/null
+++ b/modules/nf-core/umicollapse/tests/main.nf.test.snap
@@ -0,0 +1,36 @@
+{
+    "umicollapse single end test": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.dedup.bam:md5,4e86d60aa82242889ab5f9031418ab2e"
+                ]
+            ],
+            [
+                "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
+            ]
+        ],
+        "timestamp": "2024-01-24T13:57:02.801573999"
+    },
+    "umicollapse paired tests": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.dedup.bam:md5,54be836ec246073e60212445b4369a91"
+                ]
+            ],
+            [
+                "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
+            ]
+        ],
+        "timestamp": "2024-01-24T13:57:24.797928099"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/umicollapse/tests/nextflow.config b/modules/nf-core/umicollapse/tests/nextflow.config
new file mode 100644
index 00000000..844edbdc
--- /dev/null
+++ b/modules/nf-core/umicollapse/tests/nextflow.config
@@ -0,0 +1,8 @@
+process {
+    withName: UMITOOLS_EXTRACT {
+        ext.args = '--bc-pattern="NNNN"'
+    }
+    withName: UMICOLLAPSE {
+        ext.prefix = { "${meta.id}.dedup" }
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/umicollapse/tests/nextflow_PE.config b/modules/nf-core/umicollapse/tests/nextflow_PE.config
new file mode 100644
index 00000000..ae4c9632
--- /dev/null
+++ b/modules/nf-core/umicollapse/tests/nextflow_PE.config
@@ -0,0 +1,10 @@
+process {
+
+    withName: UMITOOLS_EXTRACT {
+        ext.args = '--bc-pattern="NNNN" --bc-pattern2="NNNN"'
+    }
+
+    withName: UMICOLLAPSE {
+        ext.prefix = { "${meta.id}.dedup" }
+    }
+}
diff --git a/modules/nf-core/umicollapse/tests/nextflow_SE.config b/modules/nf-core/umicollapse/tests/nextflow_SE.config
new file mode 100644
index 00000000..d4b94436
--- /dev/null
+++ b/modules/nf-core/umicollapse/tests/nextflow_SE.config
@@ -0,0 +1,10 @@
+process {
+
+    withName: UMITOOLS_EXTRACT {
+        ext.args = '--bc-pattern="NNNN"'
+    }
+
+    withName: UMICOLLAPSE {
+        ext.prefix = { "${meta.id}.dedup" }
+    }
+}
diff --git a/modules/nf-core/umicollapse/tests/tags.yml b/modules/nf-core/umicollapse/tests/tags.yml
new file mode 100644
index 00000000..912879c4
--- /dev/null
+++ b/modules/nf-core/umicollapse/tests/tags.yml
@@ -0,0 +1,2 @@
+umicollapse:
+  - "modules/nf-core/umicollapse/**"

From c38c0653a7277dc81d99336db88042065ecdd67f Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 24 Jan 2024 15:49:08 +0000
Subject: [PATCH 119/227] Bump versions to 2.3.0

---
 assets/multiqc_config.yml | 4 ++--
 nextflow.config           | 2 +-
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 11c1b997..d496eea8 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,7 +1,7 @@
 report_comment: >
-  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/tree/dev" target="_blank">nf-core/smrnaseq</a>
+  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/releases/tag/2.3.0" target="_blank">nf-core/smrnaseq</a>
   analysis pipeline. For information about how to interpret these results, please see the
-  <a href="https://nf-co.re/smrnaseq/dev/docs/output" target="_blank">documentation</a>.
+  <a href="https://nf-co.re/smrnaseq/2.3.0/docs/output" target="_blank">documentation</a>.
 
 report_section_order:
   "nf-core-smrnaseq-methods-description":
diff --git a/nextflow.config b/nextflow.config
index 0ea71080..16839d4b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -306,7 +306,7 @@ manifest {
     description     = """Small RNA-Seq Best Practice Analysis Pipeline."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version = '2.3dev'
+    version = '2.3.0'
     doi             = '10.5281/zenodo.3456879'
 }
 

From 3a4a52ddeb9c6c50f682a2faac15e8075728bb9b Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 24 Jan 2024 15:52:38 +0000
Subject: [PATCH 120/227] And add changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 10fc5720..5089c06e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.3dev - [date]
+## v2.3.0 - 2024-01-25 - Gray Zinc Dalmatian
 
 - [[#299]](https://github.com/nf-core/smrnaseq/issues/299) - Bugfix for missing inputs in BAM stats (`genome_quant.r`)
 - [[#164]](https://github.com/nf-core/smrnaseq/pull/164) - UMI Handling Feature implemented in the pipeline

From 521de8b3cc7a951cfbd6e1b99eecc2101b60531c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 25 Jan 2024 08:09:56 +0000
Subject: [PATCH 121/227] Bump the modules one last time

---
 CHANGELOG.md                                  |  23 +-
 modules.json                                  |   8 +-
 modules/nf-core/fastp/tests/main.nf.test      |   4 +-
 modules/nf-core/fastqc/tests/main.nf.test     | 265 ++++++++++++------
 .../nf-core/fastqc/tests/main.nf.test.snap    |  12 +-
 modules/nf-core/umicollapse/main.nf           |   2 +
 .../nf-core/umicollapse/tests/main.nf.test    |  68 ++++-
 .../umicollapse/tests/main.nf.test.snap       |  53 +++-
 .../tests/main.nf.test                        |  40 +--
 9 files changed, 357 insertions(+), 118 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5089c06e..75b6e128 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -24,17 +24,18 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Software dependencies
 
-| Dependency   | Old version | New version |
-| ------------ | ----------- | ----------- |
-| `multiqc`    | 1.15        | 1.19        |
-| `edgeR`      | 3.36.0      | 4.0.2       |
-| `limma`      | 3.50.0      | 3.58.1      |
-| `bioconvert` | 0.4.3       | 1.1.1       |
-| `mirdeep`    | 2.0.1       | 2.0.1.3     |
-| `seqkit`     | 2.3.1       | 2.6.1       |
-| `fastqc`     | 0.11.4      | 0.12.1      |
-| `samtools`   | 1.17        | 1.18        |
-| `umitools`   | <none>      | 1.1.4       |
+| Dependency    | Old version | New version |
+| ------------- | ----------- | ----------- |
+| `multiqc`     | 1.15        | 1.19        |
+| `edgeR`       | 3.36.0      | 4.0.2       |
+| `limma`       | 3.50.0      | 3.58.1      |
+| `bioconvert`  | 0.4.3       | 1.1.1       |
+| `mirdeep`     | 2.0.1       | 2.0.1.3     |
+| `seqkit`      | 2.3.1       | 2.6.1       |
+| `fastqc`      | 0.11.4      | 0.12.1      |
+| `samtools`    | 1.17        | 1.18        |
+| `umitools`    | <none>      | 1.1.4       |
+| `umicollapse` | <none>      | 1.0.0       |
 
 ## [v2.2.4](https://github.com/nf-core/smrnaseq/releases/tag/2.2.4) - 2023-11-03
 
diff --git a/modules.json b/modules.json
index 8236fcfd..11ca6940 100644
--- a/modules.json
+++ b/modules.json
@@ -22,12 +22,12 @@
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "1799e452de650f6fb8890d25829bca23014b0728",
+                        "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9",
                         "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "617777a807a1770f73deb38c80004bac06807eef",
+                        "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9",
                         "installed_by": ["fastq_fastqc_umitools_fastp"]
                     },
                     "multiqc": {
@@ -67,7 +67,7 @@
                     },
                     "umicollapse": {
                         "branch": "master",
-                        "git_sha": "b71a681e9ec41cefd330e12b8566b5f5aff4941c",
+                        "git_sha": "b573d74ce7eced7963d00d4a7f99db9caed61d79",
                         "installed_by": ["modules"]
                     },
                     "umitools/extract": {
@@ -91,7 +91,7 @@
                     },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
-                        "git_sha": "d20574ce7420a54fe47b46a91c5f2215d1a2777c",
+                        "git_sha": "a4bceac1aecee5aa0a5dbc601baf0e2e61013fb2",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test
index dcf4fd6f..fa7e5b4d 100644
--- a/modules/nf-core/fastp/tests/main.nf.test
+++ b/modules/nf-core/fastp/tests/main.nf.test
@@ -264,7 +264,7 @@ nextflow_process {
 
                 input[0] = Channel.of([
                     [ id:'test', single_end:true ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ]
                 ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
@@ -334,7 +334,7 @@ nextflow_process {
 
                 input[0] = Channel.of([
                     [ id:'test', single_end:true ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ]
                 ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
index b9e8f926..1f21c664 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -3,24 +3,20 @@ nextflow_process {
     name "Test Process FASTQC"
     script "../main.nf"
     process "FASTQC"
+
     tag "modules"
     tag "modules_nfcore"
     tag "fastqc"
 
-    test("Single-Read") {
+    test("sarscov2 single-end [fastq]") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id: 'test', single_end:true ],
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-                    ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
                 """
             }
         }
@@ -28,82 +24,189 @@ nextflow_process {
         then {
             assertAll (
             { assert process.success },
+
             // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
             // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
             // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
-            { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" },
-            { assert path(process.out.html.get(0).get(1)).getText().contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert snapshot(process.out.versions).match("versions") },
-            { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" }
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 interleaved [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true)
+                ])
+            """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
             )
         }
     }
-// TODO
-// //
-// // Test with paired-end data
-// //
-// workflow test_fastqc_paired_end {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 [
-//                     file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-//                 ]
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with interleaved data
-// //
-// workflow test_fastqc_interleaved {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true)
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with bam data
-// //
-// workflow test_fastqc_bam {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with multiple samples
-// //
-// workflow test_fastqc_multiple {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 [
-//                     file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true)
-//                 ]
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with custom prefix
-// //
-// workflow test_fastqc_custom_prefix {
-//     input = [
-//                 [ id:'mysample', single_end:true ], // meta map
-//                 file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-//             ]
-
-//     FASTQC ( input )
-// }
+
+    test("sarscov2 paired-end [bam]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 multiple [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+            { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" },
+            { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" },
+            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+            { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" },
+            { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" },
+            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 custom_prefix") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'mysample', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastq] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id: 'test', single_end:true ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out.html.collect { file(it[1]).getName() } +
+                                process.out.zip.collect { file(it[1]).getName() } +
+                                process.out.versions ).match() }
+            )
+        }
+    }
+
 }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
index 636a32ce..5d624bb8 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test.snap
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -1,10 +1,20 @@
 {
+    "sarscov2 single-end [fastq] - stub": {
+        "content": [
+            [
+                "test.html",
+                "test.zip",
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "timestamp": "2024-01-17T18:40:57.254299"
+    },
     "versions": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
-        "timestamp": "2023-10-09T23:40:54+0000"
+        "timestamp": "2024-01-17T18:36:50.033627"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 291d3273..15109077 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -51,8 +51,10 @@ process UMICOLLAPSE {
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '1.0.0-1'
     """
     touch ${prefix}.dedup.bam
+    touch ${prefix}_UMICollapse.log
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         umicollapse: $VERSION
diff --git a/modules/nf-core/umicollapse/tests/main.nf.test b/modules/nf-core/umicollapse/tests/main.nf.test
index 1b8bf7e9..27c3e7a1 100644
--- a/modules/nf-core/umicollapse/tests/main.nf.test
+++ b/modules/nf-core/umicollapse/tests/main.nf.test
@@ -82,7 +82,7 @@ nextflow_process {
     }
 
     test("umicollapse paired tests") {
-        setup{
+            setup{
             run("UMITOOLS_EXTRACT"){
                 script "../../umitools/extract/main.nf"
                 config "./nextflow_PE.config"
@@ -150,4 +150,70 @@ nextflow_process {
 
     }
 
+    test("umicollapse stub tests") {
+        options "-stub-run"
+            setup{
+            run("UMITOOLS_EXTRACT"){
+                script "../../umitools/extract/main.nf"
+                config "./nextflow_PE.config"
+                process{
+                    """
+                    input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
+                    ]
+                    ]
+                    """
+                }
+            }
+
+            run("BWA_INDEX"){
+                script "../../bwa/index/main.nf"
+                process{
+                    """
+                    input[0] = [
+                            [ id:'sarscov2'],
+                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                            ]
+                    """
+                }
+            }
+            run("BWA_MEM"){
+                script "../../bwa/mem/main.nf"
+                process{
+                    """
+                    input[0] = UMITOOLS_EXTRACT.out.reads
+                    input[1] = BWA_INDEX.out.index
+                    input[2] = true
+                    """
+                }
+            }
+            run("SAMTOOLS_INDEX"){
+                script "../../samtools/index/main.nf"
+                process{
+                    """
+                    input[0] = BWA_MEM.out.bam
+                    """
+                }
+            }
+        }
+        when {
+            config "./nextflow_PE.config"
+            process {
+                """
+                input[0] =  BWA_MEM.out.bam.join(SAMTOOLS_INDEX.out.bai, by: [0])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
 }
diff --git a/modules/nf-core/umicollapse/tests/main.nf.test.snap b/modules/nf-core/umicollapse/tests/main.nf.test.snap
index 60250530..8cc979aa 100644
--- a/modules/nf-core/umicollapse/tests/main.nf.test.snap
+++ b/modules/nf-core/umicollapse/tests/main.nf.test.snap
@@ -14,7 +14,56 @@
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
-        "timestamp": "2024-01-24T13:57:02.801573999"
+        "timestamp": "2024-01-24T21:34:08.702681823"
+    },
+    "umicollapse stub tests": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.dedup.dedup.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.dedup_UMICollapse.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.dedup.dedup.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.dedup_UMICollapse.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
+                ]
+            }
+        ],
+        "timestamp": "2024-01-24T21:34:48.437479265"
     },
     "umicollapse paired tests": {
         "content": [
@@ -31,6 +80,6 @@
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
-        "timestamp": "2024-01-24T13:57:24.797928099"
+        "timestamp": "2024-01-24T21:34:25.444373007"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
index 91dec8c5..8f1d82d3 100644
--- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
@@ -17,22 +17,30 @@ nextflow_workflow {
         when {
             workflow {
                 """
-                input[0] = [
-                            [ id:'test', single_end:false ], // meta map
-                            [
-                             file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                             file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-                            ]
-                ]
-                input[1] = false // skip_fastqc
-                input[2] = false // with_umi
-                input[3] = false // skip_umi_extract
-                input[4] = 1     // umi_discard_read
-                input[5] = false // skip_trimming
-                input[6] = []    // adapter_fasta
-                input[7] = false // save_trimmed_fail
-                input[8] = false // save_merged
-                input[9] = 1     // min_trimmed_reads
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
+                input[1] = skip_fastqc
+                input[2] = with_umi
+                input[3] = skip_umi_extract
+                input[4] = umi_discard_read
+                input[5] = skip_trimming
+                input[6] = adapter_fasta
+                input[7] = save_trimmed_fail
+                input[8] = save_merged
+                input[9] = min_trimmed_reads
                 """
             }
         }

From 7cd043f5f11933e9ecef373cc22c34103c5f0c84 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <apeltzer@users.noreply.github.com>
Date: Thu, 25 Jan 2024 10:25:45 +0100
Subject: [PATCH 122/227] Update nextflow.config

Co-authored-by: Maxime U Garcia <max.u.garcia@gmail.com>
---
 nextflow.config | 1 -
 1 file changed, 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 16839d4b..e613b181 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -16,7 +16,6 @@ params {
     // Workflow flags
     protocol                    = 'illumina'
 
-    // References
     // References
     genome                      = null
     igenomes_base               = 's3://ngi-igenomes/igenomes'

From 4b2bf8934b9254b09777e3a9d0bceb6b3c484031 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Fri, 26 Jan 2024 10:38:38 +0100
Subject: [PATCH 123/227] Remove redundant fastq option

---
 conf/modules.config | 10 +---------
 1 file changed, 1 insertion(+), 9 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 127e0a34..835677be 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -45,7 +45,7 @@ process {
 process {
     withName: 'CAT_FASTQ' {
         publishDir = [
-            path: { "${params.outdir}/fastq" },
+            path: { "${params.outdir}/cat_fastq" },
             mode: params.publish_dir_mode,
             pattern: '*.fastq',
             // enabled: params.save_merged_fastq //TODO ? implement save_merged_fastq param
@@ -88,14 +88,6 @@ process {
     }
 }
 
-if (!(params.skip_fastqc)) {
-    process {
-        withName: '.*:FASTQC_UMITOOLS_FASTP:FASTQC_.*' {
-            ext.args = '--quiet'
-        }
-    }
-}
-
 if (!params.skip_fastp) {
     process {
         withName: '.*:FASTQC_UMITOOLS_FASTP:FASTP' {

From 17f2ae53f6788f51de59a04839d5634cea032a8a Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Fri, 26 Jan 2024 10:47:15 +0100
Subject: [PATCH 124/227] Clean up config

Replicating the if/else logic from the main workflow is
not necessary, since now, by default
nextflow.enable.configProcessNamesValidation = false
---
 conf/modules.config | 329 ++++++++++++++++++++------------------------
 1 file changed, 151 insertions(+), 178 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 835677be..6e3f17dd 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -10,17 +10,22 @@
 ----------------------------------------------------------------------------------------
 */
 
-//
-// General configuration options
-//
+
 
 process {
+
+    //
+    // General configuration options
+    //
     publishDir = [
         path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
         mode: params.publish_dir_mode,
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
+    //
+    // Input preparation
+    //
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK' {
         publishDir = [
             path: { "${params.outdir}/pipeline_info" },
@@ -28,21 +33,6 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-
-    withName: 'CUSTOM_DUMPSOFTWAREVERSIONS' {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: params.publish_dir_mode,
-            pattern: '*_versions.yml'
-        ]
-    }
-}
-
-//
-// Genome preparation options
-//
-
-process {
     withName: 'CAT_FASTQ' {
         publishDir = [
             path: { "${params.outdir}/cat_fastq" },
@@ -51,6 +41,10 @@ process {
             // enabled: params.save_merged_fastq //TODO ? implement save_merged_fastq param
         ]
     }
+
+    //
+    // Genome preparation options
+    //
     withName: 'INDEX_GENOME' {
         publishDir = [
             path: { "${params.outdir}/index" },
@@ -72,13 +66,10 @@ process {
             enabled: true
         ]
     }
-}
 
-//
-// Read QC and trimming options
-//
-
-process {
+    //
+    // Read QC and trimming options
+    //
     withName: 'MIRTRACE_RUN' {
         publishDir = [
             path: { "${params.outdir}/mirtrace" },
@@ -86,145 +77,128 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-}
 
-if (!params.skip_fastp) {
-    process {
-        withName: '.*:FASTQC_UMITOOLS_FASTP:FASTP' {
-                    ext.args = [ "",
-            params.trim_fastq              ? "" : "--disable_adapter_trimming",
-            params.clip_r1 > 0             ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
-            params.three_prime_clip_r1 > 0 ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.
-            params.fastp_min_length > 0    ? "-l ${params.fastp_min_length}" : "",
-            params.fastp_max_length > 0    ? "--max_len1 ${params.fastp_max_length}" : "",
-            params.three_prime_adapter == null ?  '' : "--adapter_sequence ${params.three_prime_adapter}"
-        ].join(" ").trim()
-            publishDir = [
-                [
-                    path: { "${params.outdir}/fastp" },
-                    mode: params.publish_dir_mode,
-                    pattern: "*.{json,html}"
-                ],
-                [
-                    path: { "${params.outdir}/fastp/log" },
-                    mode: params.publish_dir_mode,
-                    pattern: "*.log"
-                ],
-                [
-                    path: { "${params.outdir}/fastp" },
-                    mode: params.publish_dir_mode,
-                    pattern: "*.fail.fastq.gz",
-                    enabled: params.save_trimmed_fail
-                ]
+    withName: '.*:FASTQC_UMITOOLS_FASTP:FASTP' {
+                ext.args = [ "",
+        params.trim_fastq              ? "" : "--disable_adapter_trimming",
+        params.clip_r1 > 0             ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
+        params.three_prime_clip_r1 > 0 ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.
+        params.fastp_min_length > 0    ? "-l ${params.fastp_min_length}" : "",
+        params.fastp_max_length > 0    ? "--max_len1 ${params.fastp_max_length}" : "",
+        params.three_prime_adapter == null ?  '' : "--adapter_sequence ${params.three_prime_adapter}"
+    ].join(" ").trim()
+        publishDir = [
+            [
+                path: { "${params.outdir}/fastp" },
+                mode: params.publish_dir_mode,
+                pattern: "*.{json,html}"
+            ],
+            [
+                path: { "${params.outdir}/fastp/log" },
+                mode: params.publish_dir_mode,
+                pattern: "*.log"
+            ],
+            [
+                path: { "${params.outdir}/fastp" },
+                mode: params.publish_dir_mode,
+                pattern: "*.fail.fastq.gz",
+                enabled: params.save_trimmed_fail
             ]
-        }
-
+        ]
     }
 
-    if (!params.skip_fastqc) {
-        process {
-            withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_RAW' {
-                ext.args = '--quiet'
-                publishDir = [
-                    path: { "${params.outdir}/fastqc/raw" },
-                    mode: params.publish_dir_mode,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' {
-                ext.args = '--quiet'
-                publishDir = [
-                    path: { "${params.outdir}/fastqc/trim" },
-                    mode: params.publish_dir_mode,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-}
 
-if (params.with_umi && !params.skip_umi_extract) {
-    process {
-        withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:UMITOOLS_EXTRACT' {
-            ext.args   = [
-                    params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
-                    params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
-                ].join(' ').trim()
-            publishDir = [
-                [
-                    path: { "${params.outdir}/umitools" },
-                    mode: params.publish_dir_mode,
-                    pattern: "*.log"
-                ],
-                [
-                    path: { "${params.outdir}/umitools" },
-                    mode: params.publish_dir_mode,
-                    pattern: "*.fastq.gz",
-                    enabled: params.save_umi_intermeds
-                ]
-            ]
-        }
+    withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_RAW' {
+        ext.args = '--quiet'
+        publishDir = [
+            path: { "${params.outdir}/fastqc/raw" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+    withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' {
+        ext.args = '--quiet'
+        publishDir = [
+            path: { "${params.outdir}/fastqc/trim" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
     }
-}
 
-//
-// UMI tools deduplication
-//
 
-if (params.with_umi) {
-    process {
-        withName: '.*:DEDUPLICATE_UMIS:UMICOLLAPSE' {
-            ext.args = { meta.single_end ? "--algo ${params.umitools_method} --two-pass" : "--method ${params.umitools_method} --two-pass --paired --remove-unpaired --remove-chimeric" }
-            ext.prefix = { "${meta.id}.umi_dedup.sorted" }
-            publishDir = [
-                path: { "${params.outdir}/umi_dedup" },
+    withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:UMITOOLS_EXTRACT' {
+        ext.args   = [
+                params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
+                params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
+            ].join(' ').trim()
+        publishDir = [
+            [
+                path: { "${params.outdir}/umitools" },
                 mode: params.publish_dir_mode,
-                pattern: '*.bam',
-                enabled: (
-                    params.save_umi_intermeds
-                )
-            ]
-        }
-
-        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' {
-            ext.prefix = { "${meta.id}.sorted" }
-            publishDir = [
-                path: { "${params.outdir}/umi_dedup" },
+                pattern: "*.log"
+            ],
+            [
+                path: { "${params.outdir}/umitools" },
                 mode: params.publish_dir_mode,
-                pattern: '*.{bam}',
-                enabled: (
-                    params.save_umi_intermeds
-                )
+                pattern: "*.fastq.gz",
+                enabled: params.save_umi_intermeds
             ]
-        }
+        ]
+    }
 
-        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX' {
-            ext.prefix = { "${meta.id}.sorted" }
-            publishDir = [
-                path: { "${params.outdir}/umi_dedup" },
-                mode: params.publish_dir_mode,
-                pattern: '*.{bai,csi}',
-                enabled: (
-                    params.save_umi_intermeds
-                )
-            ]
-        }
+    //
+    // UMI tools deduplication
+    //
 
-        withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:.*' {
-            publishDir = [
-                path: { "${params.outdir}/umi_dedup/samtools_stats" },
-                mode: params.publish_dir_mode,
-                pattern: '*.{stats,flagstat,idxstats}'
-            ]
-        }
+    withName: '.*:DEDUPLICATE_UMIS:UMICOLLAPSE' {
+        ext.args = { meta.single_end ? "--algo ${params.umitools_method} --two-pass" : "--method ${params.umitools_method} --two-pass --paired --remove-unpaired --remove-chimeric" }
+        ext.prefix = { "${meta.id}.umi_dedup.sorted" }
+        publishDir = [
+            path: { "${params.outdir}/umi_dedup" },
+            mode: params.publish_dir_mode,
+            pattern: '*.bam',
+            enabled: (
+                params.save_umi_intermeds
+            )
+        ]
     }
-}
 
-//
-// Quantification
-//
+    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' {
+        ext.prefix = { "${meta.id}.sorted" }
+        publishDir = [
+            path: { "${params.outdir}/umi_dedup" },
+            mode: params.publish_dir_mode,
+            pattern: '*.{bam}',
+            enabled: (
+                params.save_umi_intermeds
+            )
+        ]
+    }
+
+    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX' {
+        ext.prefix = { "${meta.id}.sorted" }
+        publishDir = [
+            path: { "${params.outdir}/umi_dedup" },
+            mode: params.publish_dir_mode,
+            pattern: '*.{bai,csi}',
+            enabled: (
+                params.save_umi_intermeds
+            )
+        ]
+    }
+
+    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:.*' {
+        publishDir = [
+            path: { "${params.outdir}/umi_dedup/samtools_stats" },
+            mode: params.publish_dir_mode,
+            pattern: '*.{stats,flagstat,idxstats}'
+        ]
+    }
+
+    //
+    // Quantification
+    //
 
-process {
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:PARSE_.*' {
     publishDir = [
             path: { "${params.outdir}/genome" },
@@ -282,10 +256,8 @@ process {
             enabled: true
         ]
     }
-}
 
 
-process {
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_.*' {
         ext.prefix = { "${meta.id}.sorted" }
         publishDir = [
@@ -310,37 +282,38 @@ process {
             pattern: "unmapped/*.gz"
         ]
     }
-}
 
-if (!params.skip_mirdeep) {
-    process {
-        withName: 'MIRDEEP2_MAPPER' {
-            publishDir = [
-                path: { "${params.outdir}/mirdeep2/mapper" },
-                mode: params.publish_dir_mode,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-        withName: 'MIRDEEP2_RUN' {
-            publishDir = [
-                path: { "${params.outdir}/mirdeep2/run" },
-                mode: params.publish_dir_mode,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
+    withName: 'MIRDEEP2_MAPPER' {
+        publishDir = [
+            path: { "${params.outdir}/mirdeep2/mapper" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
     }
-}
-
-if (!params.skip_multiqc) {
-    process {
-        withName: 'MULTIQC' {
-            ext.args   = params.multiqc_title ? "--title \"$params.multiqc_title\"" : ''
-            publishDir = [
-                path: { "${params.outdir}/multiqc" },
-                mode: params.publish_dir_mode,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
+    withName: 'MIRDEEP2_RUN' {
+        publishDir = [
+            path: { "${params.outdir}/mirdeep2/run" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
     }
 
+    //
+    // reports
+    //
+    withName: 'CUSTOM_DUMPSOFTWAREVERSIONS' {
+        publishDir = [
+            path: { "${params.outdir}/pipeline_info" },
+            mode: params.publish_dir_mode,
+            pattern: '*_versions.yml'
+        ]
+    }
+    withName: 'MULTIQC' {
+        ext.args   = params.multiqc_title ? "--title \"$params.multiqc_title\"" : ''
+        publishDir = [
+            path: { "${params.outdir}/multiqc" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
 }

From 306cd4e072cba60111196f01b6556b584d7f4d6b Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Fri, 26 Jan 2024 14:06:05 +0100
Subject: [PATCH 125/227] WIP reorganize config

---
 conf/modules.config | 133 ++++++++++++++++++++++++--------------------
 1 file changed, 74 insertions(+), 59 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 6e3f17dd..bcc0f018 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -43,50 +43,17 @@ process {
     }
 
     //
-    // Genome preparation options
+    // FASTQ_FASTQC_UMITOOLS_FASTP
     //
-    withName: 'INDEX_GENOME' {
-        publishDir = [
-            path: { "${params.outdir}/index" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:FORMAT_.*' {
-        publishDir = [
-            path: { "${params.outdir}/genome" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-    withName: 'EDGER_QC' {
-        publishDir = [
-            path: { "${params.outdir}/edger" },
-            mode: params.publish_dir_mode,
-            enabled: true
-        ]
-    }
-
-    //
-    // Read QC and trimming options
-    //
-    withName: 'MIRTRACE_RUN' {
-        publishDir = [
-            path: { "${params.outdir}/mirtrace" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
     withName: '.*:FASTQC_UMITOOLS_FASTP:FASTP' {
-                ext.args = [ "",
-        params.trim_fastq              ? "" : "--disable_adapter_trimming",
-        params.clip_r1 > 0             ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
-        params.three_prime_clip_r1 > 0 ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.
-        params.fastp_min_length > 0    ? "-l ${params.fastp_min_length}" : "",
-        params.fastp_max_length > 0    ? "--max_len1 ${params.fastp_max_length}" : "",
-        params.three_prime_adapter == null ?  '' : "--adapter_sequence ${params.three_prime_adapter}"
-    ].join(" ").trim()
+        ext.args = [ "",
+            params.trim_fastq              ? "" : "--disable_adapter_trimming",
+            params.clip_r1 > 0             ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
+            params.three_prime_clip_r1 > 0 ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.
+            params.fastp_min_length > 0    ? "-l ${params.fastp_min_length}" : "",
+            params.fastp_max_length > 0    ? "--max_len1 ${params.fastp_max_length}" : "",
+            params.three_prime_adapter == null ?  '' : "--adapter_sequence ${params.three_prime_adapter}"
+        ].join(" ").trim()
         publishDir = [
             [
                 path: { "${params.outdir}/fastp" },
@@ -106,8 +73,6 @@ process {
             ]
         ]
     }
-
-
     withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_RAW' {
         ext.args = '--quiet'
         publishDir = [
@@ -119,18 +84,16 @@ process {
     withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' {
         ext.args = '--quiet'
         publishDir = [
-            path: { "${params.outdir}/fastqc/trim" },
+            path: { "${params.outdir}/fastqc/trimmed" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-
-
     withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:UMITOOLS_EXTRACT' {
-        ext.args   = [
-                params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
-                params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
-            ].join(' ').trim()
+        ext.args  = [
+            params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
+            params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
+        ].join(' ').trim()
         publishDir = [
             [
                 path: { "${params.outdir}/umitools" },
@@ -147,9 +110,19 @@ process {
     }
 
     //
-    // UMI tools deduplication
+    // Make bowtie index
     //
+    withName: 'INDEX_GENOME' {
+        publishDir = [
+            path: { "${params.outdir}/bowtie_index" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
 
+    //
+    // UMI deduplication
+    //
     withName: '.*:DEDUPLICATE_UMIS:UMICOLLAPSE' {
         ext.args = { meta.single_end ? "--algo ${params.umitools_method} --two-pass" : "--method ${params.umitools_method} --two-pass --paired --remove-unpaired --remove-chimeric" }
         ext.prefix = { "${meta.id}.umi_dedup.sorted" }
@@ -163,6 +136,54 @@ process {
         ]
     }
 
+    //
+    // MIRTRACE QC
+    //
+    withName: 'MIRTRACE_RUN' {
+        publishDir = [
+            path: { "${params.outdir}/mirtrace" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
+    //
+    // CONTAMINANT_FILTER
+    //
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:CONTAMINANT_FILTER:.*' {
+        publishDir = [
+            path: { "${params.outdir}/contaminant_filter/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
+
+
+    //----
+
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:FORMAT_.*' {
+        publishDir = [
+            path: { "${params.outdir}/genome" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+    withName: 'EDGER_QC' {
+        publishDir = [
+            path: { "${params.outdir}/edger" },
+            mode: params.publish_dir_mode,
+            enabled: true
+        ]
+    }
+
+
+    //
+    // UMI tools deduplication
+    //
+
+
+
     withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' {
         ext.prefix = { "${meta.id}.sorted" }
         publishDir = [
@@ -249,13 +270,7 @@ process {
             pattern: "*.tsv"
         ]
     }
-    withName: 'EDGER_QC' {
-        publishDir = [
-            path: { "${params.outdir}/edger" },
-            mode: params.publish_dir_mode,
-            enabled: true
-        ]
-    }
+
 
 
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_.*' {

From ede1ee9ca78f710033bea6ba10ce13ccc90aa011 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Fri, 26 Jan 2024 14:32:17 +0100
Subject: [PATCH 126/227] More config updates

---
 conf/modules.config | 162 ++++++++++++++++++++++++--------------------
 1 file changed, 88 insertions(+), 74 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index bcc0f018..b6edc20c 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -96,12 +96,12 @@ process {
         ].join(' ').trim()
         publishDir = [
             [
-                path: { "${params.outdir}/umitools" },
+                path: { "${params.outdir}/umi_dedup/fastq_extracted_umi" },
                 mode: params.publish_dir_mode,
                 pattern: "*.log"
             ],
             [
-                path: { "${params.outdir}/umitools" },
+                path: { "${params.outdir}/umi_dedup/fastq_extracted_umi" },
                 mode: params.publish_dir_mode,
                 pattern: "*.fastq.gz",
                 enabled: params.save_umi_intermeds
@@ -114,7 +114,7 @@ process {
     //
     withName: 'INDEX_GENOME' {
         publishDir = [
-            path: { "${params.outdir}/bowtie_index" },
+            path: { "${params.outdir}/bowtie_index/genome" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -123,11 +123,50 @@ process {
     //
     // UMI deduplication
     //
+    withName: '.*:DEDUPLICATE_UMIS:UMI_MAP_GENOME' {
+        publishDir = [
+            path: { "${params.outdir}/umi_dedup/bam_mapped" },
+            mode: params.publish_dir_mode,
+            pattern: '*.bam',
+            enabled: (
+                params.save_umi_intermeds
+            )
+        ]
+    }
+    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' {
+        ext.prefix = { "${meta.id}.sorted" }
+        publishDir = [
+            path: { "${params.outdir}/umi_dedup/bam_mapped_sorted" },
+            mode: params.publish_dir_mode,
+            pattern: '*.{bam}',
+            enabled: (
+                params.save_umi_intermeds
+            )
+        ]
+    }
+    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX' {
+        ext.prefix = { "${meta.id}.sorted" }
+        publishDir = [
+            path: { "${params.outdir}/umi_dedup/bam_mapped_sorted" },
+            mode: params.publish_dir_mode,
+            pattern: '*.{bai,csi}',
+            enabled: (
+                params.save_umi_intermeds
+            )
+        ]
+    }
+    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:.*' {
+        publishDir = [
+            path: { "${params.outdir}/umi_dedup/bam_mapped_sorted" },
+            mode: params.publish_dir_mode,
+            pattern: '*.{stats,flagstat,idxstats}'
+        ]
+    }
     withName: '.*:DEDUPLICATE_UMIS:UMICOLLAPSE' {
         ext.args = { meta.single_end ? "--algo ${params.umitools_method} --two-pass" : "--method ${params.umitools_method} --two-pass --paired --remove-unpaired --remove-chimeric" }
         ext.prefix = { "${meta.id}.umi_dedup.sorted" }
         publishDir = [
-            path: { "${params.outdir}/umi_dedup" },
+            path: { "${params.outdir}/bam_deduplicated" },
             mode: params.publish_dir_mode,
             pattern: '*.bam',
             enabled: (
@@ -135,6 +174,15 @@ process {
             )
         ]
     }
+    withName: '.*:DEDUPLICATE_UMIS:SAMTOOLS_BAM2FQ' {
+        publishDir = [
+            path: { "${params.outdir}/umi_dedup/fastq_deduplicated" },
+            mode: params.publish_dir_mode,
+            enabled: (
+                params.save_umi_intermeds
+            )
+        ]
+    }
 
     //
     // MIRTRACE QC
@@ -158,146 +206,112 @@ process {
         ]
     }
 
-
-
-    //----
-
+    //
+    // MIRNA_QUANT
+    //
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:FORMAT_.*' {
         publishDir = [
-            path: { "${params.outdir}/genome" },
+            path: { "${params.outdir}/mirna_quant/reference" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'EDGER_QC' {
-        publishDir = [
-            path: { "${params.outdir}/edger" },
-            mode: params.publish_dir_mode,
-            enabled: true
-        ]
-    }
-
-
-    //
-    // UMI tools deduplication
-    //
-
-
-
-    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' {
-        ext.prefix = { "${meta.id}.sorted" }
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:PARSE_.*' {
         publishDir = [
-            path: { "${params.outdir}/umi_dedup" },
+            path: { "${params.outdir}/mirna_quant/reference" },
             mode: params.publish_dir_mode,
-            pattern: '*.{bam}',
-            enabled: (
-                params.save_umi_intermeds
-            )
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-
-    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX' {
-        ext.prefix = { "${meta.id}.sorted" }
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:INDEX_MATURE' {
         publishDir = [
-            path: { "${params.outdir}/umi_dedup" },
+            path: { "${params.outdir}/bowtie_index/mirna_mature" },
             mode: params.publish_dir_mode,
-            pattern: '*.{bai,csi}',
-            enabled: (
-                params.save_umi_intermeds
-            )
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-
-    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:.*' {
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:INDEX_HAIRPIN' {
         publishDir = [
-            path: { "${params.outdir}/umi_dedup/samtools_stats" },
-            mode: params.publish_dir_mode,
-            pattern: '*.{stats,flagstat,idxstats}'
-        ]
-    }
-
-    //
-    // Quantification
-    //
-
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:PARSE_.*' {
-    publishDir = [
-            path: { "${params.outdir}/genome" },
+            path: { "${params.outdir}/bowtie_index/mirna_hairpin" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BAM_STATS_.*:SAMTOOLS_SORT' {
-        ext.prefix = { "${meta.id}.sorted" }
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:(BOWTIE_MAP_MATURE|BAM_STATS_MATURE).*' {
         publishDir = [
-            path: { "${params.outdir}/samtools" },
+            path: { "${params.outdir}/mirna_quant/bam/mature" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BAM_STATS_.*:BAM_STATS_SAMTOOLS:.*' {
-        ext.prefix = { "${meta.id}.sorted" }
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:(BOWTIE_MAP_HAIRPIN|BAM_STATS_HAIRPIN).*' {
         publishDir = [
-            path: { "${params.outdir}/samtools/samtools_stats" },
+            path: { "${params.outdir}/mirna_quant/bam/hairpin" },
             mode: params.publish_dir_mode,
-            pattern: "*.{stats,flagstat,idxstats}"
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_.*' {
+    withName: 'EDGER_QC' {
         publishDir = [
-            path: { "${params.outdir}/unmapped/fastq" },
+            path: { "${params.outdir}/mirna_quant/edger_qc" },
             mode: params.publish_dir_mode,
-            pattern: "unmapped/*.gz"
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
     withName: 'SEQCLUSTER_SEQUENCES' {
         publishDir = [
-            path: { "${params.outdir}/seqcluster" },
-            enabled: false
+            path: { "${params.outdir}/mirna_quant/seqcluster" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
     withName: 'MIRTOP_QUANT' {
         publishDir = [
-            path: { "${params.outdir}" },
+            path: { "${params.outdir}/mirna_quant/mirtop" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:TABLE_MERGE' {
         publishDir = [
-            path: { "${params.outdir}/mirtop" },
+            path: { "${params.outdir}/mirna_quant/mirtop" },
             mode: params.publish_dir_mode,
             pattern: "*.tsv"
         ]
     }
 
 
-
+    //
+    // GENOME_QUANT
+    //
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_.*' {
         ext.prefix = { "${meta.id}.sorted" }
         publishDir = [
-            path: { "${params.outdir}/samtools" },
+            path: { "${params.outdir}/genome_quant/bam" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:.*' {
         ext.prefix = { "${meta.id}.sorted" }
         publishDir = [
-            path: { "${params.outdir}/samtools/samtools_stats" },
+            path: { "${params.outdir}/genome_quant/bam" },
             mode: params.publish_dir_mode,
             pattern: "*.{stats,flagstat,idxstats}"
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BOWTIE_MAP_.*' {
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BOWTIE_MAP_GENOME' {
         publishDir = [
-            path: { "${params.outdir}/unmapped/fastq" },
+            path: { "${params.outdir}/unmapped/bam" },
             mode: params.publish_dir_mode,
             pattern: "unmapped/*.gz"
         ]
     }
 
+
+    //
+    // MIRDEEP
+    //
     withName: 'MIRDEEP2_MAPPER' {
         publishDir = [
             path: { "${params.outdir}/mirdeep2/mapper" },

From e707e28a4ad0c55acf1b95a76ff3ad9144e29a0c Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Fri, 26 Jan 2024 14:49:14 +0100
Subject: [PATCH 127/227] Final outputpath fixes

---
 conf/modules.config | 44 +++++++++++++++++++++++++++++++++++---------
 1 file changed, 35 insertions(+), 9 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index b6edc20c..b57e4f92 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -73,7 +73,7 @@ process {
             ]
         ]
     }
-    withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_RAW' {
+    withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_RAW' {
         ext.args = '--quiet'
         publishDir = [
             path: { "${params.outdir}/fastqc/raw" },
@@ -81,7 +81,7 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: '.*:.*:FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' {
+    withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' {
         ext.args = '--quiet'
         publishDir = [
             path: { "${params.outdir}/fastqc/trimmed" },
@@ -166,7 +166,7 @@ process {
         ext.args = { meta.single_end ? "--algo ${params.umitools_method} --two-pass" : "--method ${params.umitools_method} --two-pass --paired --remove-unpaired --remove-chimeric" }
         ext.prefix = { "${meta.id}.umi_dedup.sorted" }
         publishDir = [
-            path: { "${params.outdir}/bam_deduplicated" },
+            path: { "${params.outdir}/umi_dedup/bam_deduplicated" },
             mode: params.publish_dir_mode,
             pattern: '*.bam',
             enabled: (
@@ -178,6 +178,7 @@ process {
         publishDir = [
             path: { "${params.outdir}/umi_dedup/fastq_deduplicated" },
             mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
             enabled: (
                 params.save_umi_intermeds
             )
@@ -189,7 +190,8 @@ process {
     //
     withName: 'MIRTRACE_RUN' {
         publishDir = [
-            path: { "${params.outdir}/mirtrace" },
+            //"mirtrace" already part of the published folder
+            path: { "${params.outdir}" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -237,14 +239,30 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:(BOWTIE_MAP_MATURE|BAM_STATS_MATURE).*' {
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_MATURE' {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/mature" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:(BOWTIE_MAP_HAIRPIN|BAM_STATS_HAIRPIN).*' {
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BAM_STATS_MATURE:.*' {
+        ext.prefix = { "${meta.id}.sorted" }
+        publishDir = [
+            path: { "${params.outdir}/mirna_quant/bam/mature" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_HAIRPIN' {
+        publishDir = [
+            path: { "${params.outdir}/mirna_quant/bam/hairpin" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BAM_STATS_HAIRPIN:.*' {
+        ext.prefix = { "${meta.id}.sorted" }
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/hairpin" },
             mode: params.publish_dir_mode,
@@ -265,9 +283,17 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
+    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_SEQCLUSTER' {
+        publishDir = [
+            path: { "${params.outdir}/mirna_quant/bam/seqcluster" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
     withName: 'MIRTOP_QUANT' {
         publishDir = [
-            path: { "${params.outdir}/mirna_quant/mirtop" },
+            //mirtop already part of the output folder
+            path: { "${params.outdir}/mirna_quant/" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -302,9 +328,9 @@ process {
     }
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BOWTIE_MAP_GENOME' {
         publishDir = [
-            path: { "${params.outdir}/unmapped/bam" },
+            path: { "${params.outdir}/genome_quant/bam" },
             mode: params.publish_dir_mode,
-            pattern: "unmapped/*.gz"
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
 

From a001dbf0eb36f01d99f401eaa356950301916f93 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Fri, 26 Jan 2024 15:43:44 +0100
Subject: [PATCH 128/227] Include MultiQC report for deduplicated fastqs

---
 assets/multiqc_config.yml       | 18 ++++++++++++++++++
 conf/modules.config             | 13 +++++++++++++
 subworkflows/local/umi_dedup.nf |  7 ++++++-
 workflows/smrnaseq.nf           |  2 ++
 4 files changed, 39 insertions(+), 1 deletion(-)

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index d496eea8..20ffd094 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -12,3 +12,21 @@ report_section_order:
     order: -1002
 
 export_plots: true
+
+module_order:
+   - fastqc:
+      name: "FastQC (raw)"
+      info: "This section of the report shows FastQC results on the input files."
+      path_filters:
+        - "**/*.raw_fastqc.zip"
+   - fastqc:
+      name: "FastQC (trimmed)"
+      info: "This section of the report shows FastQC results after adapter trimming."
+      path_filters:
+        - "**/*.trim_fastqc.zip"
+   - fastqc:
+      name: "FastQC (deduplicated)"
+      info: "This section of the report shows FastQC results after UMI-based deduplication."
+      path_filters:
+        - "**/*.deduplicated_fastqc.zip"
+
diff --git a/conf/modules.config b/conf/modules.config
index b57e4f92..cbd7d5d1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -74,6 +74,8 @@ process {
         ]
     }
     withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_RAW' {
+        //the prefix is required for multiqc to pickup the files separately from the other fastqc instances
+        ext.prefix =  { "${meta.id}.raw" }
         ext.args = '--quiet'
         publishDir = [
             path: { "${params.outdir}/fastqc/raw" },
@@ -82,6 +84,7 @@ process {
         ]
     }
     withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' {
+        ext.prefix =  { "${meta.id}.trim" }
         ext.args = '--quiet'
         publishDir = [
             path: { "${params.outdir}/fastqc/trimmed" },
@@ -184,6 +187,16 @@ process {
             )
         ]
     }
+    withName: '.*:DEDUPLICATE_UMIS:FASTQC_DEDUPLICATED' {
+        //the prefix is required for multiqc to pickup the files separately from the other fastqc instances
+        ext.prefix =  { "${meta.id}.deduplicated" }
+        ext.args = '--quiet'
+        publishDir = [
+            path: { "${params.outdir}/fastqc/deduplicated" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
 
     //
     // MIRTRACE QC
diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
index 9f65fa8e..2c7b533b 100644
--- a/subworkflows/local/umi_dedup.nf
+++ b/subworkflows/local/umi_dedup.nf
@@ -8,6 +8,7 @@ include { BAM_SORT_STATS_SAMTOOLS             } from '../../subworkflows/nf-core
 include { UMICOLLAPSE                         } from '../../modules/nf-core/umicollapse/main'
 include { SAMTOOLS_BAM2FQ                     } from '../../modules/nf-core/samtools/bam2fq/main'
 include { CAT_CAT                             } from '../../modules/nf-core/cat/cat/main'
+include { FASTQC as FASTQC_DEDUPLICATED        } from '../../modules/nf-core/fastqc/main'
 
 
 workflow DEDUPLICATE_UMIS {
@@ -34,8 +35,12 @@ workflow DEDUPLICATE_UMIS {
 
     ch_dedup_reads = SAMTOOLS_BAM2FQ.out.reads
 
+    FASTQC_DEDUPLICATED(ch_dedup_reads)
+
     emit:
-    reads    = ch_dedup_reads
+    reads = ch_dedup_reads
+    fastqc_html = FASTQC_DEDUPLICATED.out.html
+    fastqc_zip = FASTQC_DEDUPLICATED.out.zip
     indices  = bt_index
     versions = ch_versions
 }
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 58a4e8ad..1a7b4e64 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -272,7 +272,9 @@ workflow SMRNASEQ {
         ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
         ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_json.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(DEDUPLICATE_UMIS.out.fastqc_zip.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(contamination_stats.collect().ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(genome_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mature_stats.collect({it[1]}).ifEmpty([]))

From ec22f390cd9a534ad760f1320f9de565a602eea7 Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Fri, 26 Jan 2024 15:47:54 +0100
Subject: [PATCH 129/227] Update CHANGELOG

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 75b6e128..be1d0138 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -5,6 +5,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## v2.3.0 - 2024-01-25 - Gray Zinc Dalmatian
 
+- [[#307]](https://github.com/nf-core/smrnaseq/pull/307) - Clean up config file and improve output folder structure
 - [[#299]](https://github.com/nf-core/smrnaseq/issues/299) - Bugfix for missing inputs in BAM stats (`genome_quant.r`)
 - [[#164]](https://github.com/nf-core/smrnaseq/pull/164) - UMI Handling Feature implemented in the pipeline
 - [[#302]](https://github.com/nf-core/smrnaseq/pull/302) - Merged in nf-core template v2.11.1

From 7409e095a46b798bc96c150c1dbb32d62f2733ce Mon Sep 17 00:00:00 2001
From: Gregor Sturm <gregor.sturm@boehringer-ingelheim.com>
Date: Fri, 26 Jan 2024 15:49:33 +0100
Subject: [PATCH 130/227] Make sure multiqc can be generated when there's no
 UMI fastqc report

---
 workflows/smrnaseq.nf | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 1a7b4e64..72852fe5 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -274,7 +274,9 @@ workflow SMRNASEQ {
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_json.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files = ch_multiqc_files.mix(DEDUPLICATE_UMIS.out.fastqc_zip.collect{it[1]}.ifEmpty([]))
+        if(params.with_umi) {
+            ch_multiqc_files = ch_multiqc_files.mix(DEDUPLICATE_UMIS.out.fastqc_zip.collect{it[1]}.ifEmpty([]))
+        }
         ch_multiqc_files = ch_multiqc_files.mix(contamination_stats.collect().ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(genome_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mature_stats.collect({it[1]}).ifEmpty([]))

From 8dbd13906a176025f8f26f04b8b92ed21e344a0e Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Fri, 26 Jan 2024 14:52:24 +0000
Subject: [PATCH 131/227] [automated] Fix linting with Prettier

---
 assets/multiqc_config.yml | 7 +++----
 1 file changed, 3 insertions(+), 4 deletions(-)

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 20ffd094..2cc08ad8 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -14,19 +14,18 @@ report_section_order:
 export_plots: true
 
 module_order:
-   - fastqc:
+  - fastqc:
       name: "FastQC (raw)"
       info: "This section of the report shows FastQC results on the input files."
       path_filters:
         - "**/*.raw_fastqc.zip"
-   - fastqc:
+  - fastqc:
       name: "FastQC (trimmed)"
       info: "This section of the report shows FastQC results after adapter trimming."
       path_filters:
         - "**/*.trim_fastqc.zip"
-   - fastqc:
+  - fastqc:
       name: "FastQC (deduplicated)"
       info: "This section of the report shows FastQC results after UMI-based deduplication."
       path_filters:
         - "**/*.deduplicated_fastqc.zip"
-

From 3097d172f208ee5a9629d3475ff6aa4c95eca0f7 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Mon, 29 Jan 2024 15:13:21 +0000
Subject: [PATCH 132/227] Template update for nf-core/tools version 2.12

---
 .editorconfig                                 |   8 +
 .github/workflows/awsfulltest.yml             |   2 +-
 .github/workflows/awstest.yml                 |   2 +-
 .github/workflows/branch.yml                  |   2 +-
 .github/workflows/clean-up.yml                |   2 +-
 .github/workflows/download_pipeline.yml       |  67 +++++
 .github/workflows/fix-linting.yml             |  72 +++--
 .github/workflows/linting.yml                 |  61 +---
 .github/workflows/linting_comment.yml         |   2 +-
 .github/workflows/release-announcements.yml   |   4 +-
 .gitpod.yml                                   |   1 +
 .pre-commit-config.yaml                       |   7 +-
 CHANGELOG.md                                  |   2 +-
 README.md                                     |   8 +-
 assets/email_template.html                    |   2 +-
 assets/email_template.txt                     |   2 +-
 assets/multiqc_config.yml                     |   6 +-
 assets/nf-core-smrnaseq_logo_light.png        | Bin 74188 -> 77287 bytes
 docs/images/nf-core-smrnaseq_logo_dark.png    | Bin 74306 -> 29308 bytes
 docs/images/nf-core-smrnaseq_logo_light.png   | Bin 74188 -> 25106 bytes
 lib/WorkflowMain.groovy                       |  16 +-
 lib/nfcore_external_java_deps.jar             | Bin 2291171 -> 0 bytes
 main.nf                                       |   2 +-
 modules.json                                  |   6 +-
 .../dumpsoftwareversions/environment.yml      |   2 +-
 .../custom/dumpsoftwareversions/main.nf       |   4 +-
 .../dumpsoftwareversions/tests/main.nf.test   |   7 +-
 .../tests/main.nf.test.snap                   |  50 ++--
 modules/nf-core/fastqc/tests/main.nf.test     | 265 ++++++++++++------
 .../nf-core/fastqc/tests/main.nf.test.snap    |  12 +-
 modules/nf-core/multiqc/environment.yml       |   2 +-
 modules/nf-core/multiqc/main.nf               |   6 +-
 modules/nf-core/multiqc/meta.yml              |   1 -
 modules/nf-core/multiqc/tests/main.nf.test    |  48 +++-
 .../nf-core/multiqc/tests/main.nf.test.snap   |  21 ++
 nextflow.config                               |   5 +-
 pyproject.toml                                |  17 +-
 workflows/smrnaseq.nf                         |   7 +
 38 files changed, 496 insertions(+), 225 deletions(-)
 create mode 100644 .github/workflows/download_pipeline.yml
 delete mode 100644 lib/nfcore_external_java_deps.jar
 create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap

diff --git a/.editorconfig b/.editorconfig
index b6b31907..9b990088 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -22,3 +22,11 @@ indent_size = unset
 
 [/assets/email*]
 indent_size = unset
+
+# ignore Readme
+[README.md]
+indent_style = unset
+
+# ignore python
+[*.{py}]
+indent_style = unset
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index f8707f63..b849392a 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -31,7 +31,7 @@ jobs:
             }
           profiles: test_full
 
-      - uses: actions/upload-artifact@v3
+      - uses: actions/upload-artifact@v4
         with:
           name: Tower debug log file
           path: |
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 84d22f1c..5386cbc0 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -25,7 +25,7 @@ jobs:
             }
           profiles: test
 
-      - uses: actions/upload-artifact@v3
+      - uses: actions/upload-artifact@v4
         with:
           name: Tower debug log file
           path: |
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 96080391..e6f6941e 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -19,7 +19,7 @@ jobs:
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
       - name: Post PR comment
         if: failure()
-        uses: mshick/add-pr-comment@v1
+        uses: mshick/add-pr-comment@v2
         with:
           message: |
             ## This PR is against the `master` branch :x:
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
index 694e90ec..e37cfda5 100644
--- a/.github/workflows/clean-up.yml
+++ b/.github/workflows/clean-up.yml
@@ -10,7 +10,7 @@ jobs:
       issues: write
       pull-requests: write
     steps:
-      - uses: actions/stale@v7
+      - uses: actions/stale@v9
         with:
           stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
           stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
new file mode 100644
index 00000000..8611458a
--- /dev/null
+++ b/.github/workflows/download_pipeline.yml
@@ -0,0 +1,67 @@
+name: Test successful pipeline download with 'nf-core download'
+
+# Run the workflow when:
+#  - dispatched manually
+#  - when a PR is opened or reopened to master branch
+#  - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev.
+on:
+  workflow_dispatch:
+  pull_request:
+    types:
+      - opened
+    branches:
+      - master
+  pull_request_target:
+    branches:
+      - master
+
+env:
+  NXF_ANSI_LOG: false
+
+jobs:
+  download:
+    runs-on: ubuntu-latest
+    steps:
+      - name: Install Nextflow
+        uses: nf-core/setup-nextflow@v1
+
+      - uses: actions/setup-python@v5
+        with:
+          python-version: "3.11"
+          architecture: "x64"
+      - uses: eWaterCycle/setup-singularity@v7
+        with:
+          singularity-version: 3.8.3
+
+      - name: Install dependencies
+        run: |
+          python -m pip install --upgrade pip
+          pip install git+https://github.com/nf-core/tools.git@dev
+
+      - name: Get the repository name and current branch set as environment variable
+        run: |
+          echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV}
+          echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV}
+          echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV}
+
+      - name: Download the pipeline
+        env:
+          NXF_SINGULARITY_CACHEDIR: ./
+        run: |
+          nf-core download  ${{ env.REPO_LOWERCASE }} \
+          --revision ${{ env.REPO_BRANCH }} \
+          --outdir ./${{ env.REPOTITLE_LOWERCASE }} \
+          --compress "none" \
+          --container-system 'singularity' \
+          --container-library "quay.io" -l "docker.io" -l "ghcr.io" \
+          --container-cache-utilisation 'amend' \
+          --download-configuration
+
+      - name: Inspect download
+        run: tree ./${{ env.REPOTITLE_LOWERCASE }}
+
+      - name: Run the downloaded pipeline
+        env:
+          NXF_SINGULARITY_CACHEDIR: ./
+          NXF_SINGULARITY_HOME_MOUNT: true
+        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results 
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index 0cd44ed6..56151e57 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -4,7 +4,7 @@ on:
     types: [created]
 
 jobs:
-  deploy:
+  fix-linting:
     # Only run if comment is on a PR with the main repo, and if it contains the magic keywords
     if: >
       contains(github.event.comment.html_url, '/pull/') &&
@@ -13,10 +13,17 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
+      # indication that the linting is being fixed
+      - name: React on comment
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: eyes
+
       # Action runs on the issue comment, so we don't get the PR by default
       # Use the gh cli to check out the PR
       - name: Checkout Pull Request
@@ -24,32 +31,59 @@ jobs:
         env:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
-      - uses: actions/setup-node@v4
+      # Install and run pre-commit
+      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+        with:
+          python-version: 3.11
 
-      - name: Install Prettier
-        run: npm install -g prettier @prettier/plugin-php
+      - name: Install pre-commit
+        run: pip install pre-commit
 
-      # Check that we actually need to fix something
-      - name: Run 'prettier --check'
-        id: prettier_status
-        run: |
-          if prettier --check ${GITHUB_WORKSPACE}; then
-            echo "result=pass" >> $GITHUB_OUTPUT
-          else
-            echo "result=fail" >> $GITHUB_OUTPUT
-          fi
+      - name: Run pre-commit
+        id: pre-commit
+        run: pre-commit run --all-files
+        continue-on-error: true
 
-      - name: Run 'prettier --write'
-        if: steps.prettier_status.outputs.result == 'fail'
-        run: prettier --write ${GITHUB_WORKSPACE}
+      # indication that the linting has finished
+      - name: react if linting finished succesfully
+        if: steps.pre-commit.outcome == 'success'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: "+1"
 
       - name: Commit & push changes
-        if: steps.prettier_status.outputs.result == 'fail'
+        id: commit-and-push
+        if: steps.pre-commit.outcome == 'failure'
         run: |
           git config user.email "core@nf-co.re"
           git config user.name "nf-core-bot"
           git config push.default upstream
           git add .
           git status
-          git commit -m "[automated] Fix linting with Prettier"
+          git commit -m "[automated] Fix code linting"
           git push
+
+      - name: react if linting errors were fixed
+        id: react-if-fixed
+        if: steps.commit-and-push.outcome == 'success'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: hooray
+
+      - name: react if linting errors were not fixed
+        if: steps.commit-and-push.outcome == 'failure'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          comment-id: ${{ github.event.comment.id }}
+          reactions: confused
+
+      - name: react if linting errors were not fixed
+        if: steps.commit-and-push.outcome  == 'failure'
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
+        with:
+          issue-number: ${{ github.event.issue.number }}
+          body: |
+            @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually.
+            See [CI log](https://github.com/nf-core/smrnaseq/actions/runs/${{ github.run_id }}) for more details.
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 905c58e4..81cd098e 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -11,61 +11,22 @@ on:
     types: [published]
 
 jobs:
-  EditorConfig:
+  pre-commit:
     runs-on: ubuntu-latest
     steps:
       - uses: actions/checkout@v4
 
-      - uses: actions/setup-node@v4
-
-      - name: Install editorconfig-checker
-        run: npm install -g editorconfig-checker
-
-      - name: Run ECLint check
-        run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile')
-
-  Prettier:
-    runs-on: ubuntu-latest
-    steps:
-      - uses: actions/checkout@v4
-
-      - uses: actions/setup-node@v4
-
-      - name: Install Prettier
-        run: npm install -g prettier
-
-      - name: Run Prettier --check
-        run: prettier --check ${GITHUB_WORKSPACE}
-
-  PythonBlack:
-    runs-on: ubuntu-latest
-    steps:
-      - uses: actions/checkout@v4
-
-      - name: Check code lints with Black
-        uses: psf/black@stable
-
-      # If the above check failed, post a comment on the PR explaining the failure
-      - name: Post PR comment
-        if: failure()
-        uses: mshick/add-pr-comment@v1
+      - name: Set up Python 3.11
+        uses: actions/setup-python@v5
         with:
-          message: |
-            ## Python linting (`black`) is failing
-
-            To keep the code consistent with lots of contributors, we run automated code consistency checks.
-            To fix this CI test, please run:
-
-            * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black`
-            * Fix formatting errors in your pipeline: `black .`
-
-            Once you push these changes the test should pass, and you can hide this comment :+1:
+          python-version: 3.11
+          cache: "pip"
 
-            We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help!
+      - name: Install pre-commit
+        run: pip install pre-commit
 
-            Thanks again for your contribution!
-          repo-token: ${{ secrets.GITHUB_TOKEN }}
-          allow-repeats: false
+      - name: Run pre-commit
+        run: pre-commit run --all-files
 
   nf-core:
     runs-on: ubuntu-latest
@@ -76,7 +37,7 @@ jobs:
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v1
 
-      - uses: actions/setup-python@v4
+      - uses: actions/setup-python@v5
         with:
           python-version: "3.11"
           architecture: "x64"
@@ -99,7 +60,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@v3
+        uses: actions/upload-artifact@v4
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 0bbcd30f..147bcd10 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@v2
+        uses: dawidd6/action-download-artifact@v3
         with:
           workflow: linting.yml
           workflow_conclusion: completed
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 6ad33927..21ac3f06 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -24,7 +24,7 @@ jobs:
     runs-on: ubuntu-latest
 
     steps:
-      - uses: actions/setup-python@v4
+      - uses: actions/setup-python@v5
         with:
           python-version: "3.10"
       - name: Install dependencies
@@ -56,7 +56,7 @@ jobs:
   bsky-post:
     runs-on: ubuntu-latest
     steps:
-      - uses: zentered/bluesky-post-action@v0.0.2
+      - uses: zentered/bluesky-post-action@v0.1.0
         with:
           post: |
             Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
diff --git a/.gitpod.yml b/.gitpod.yml
index acf72695..363d5b1d 100644
--- a/.gitpod.yml
+++ b/.gitpod.yml
@@ -7,6 +7,7 @@ tasks:
   - name: unset JAVA_TOOL_OPTIONS
     command: |
       unset JAVA_TOOL_OPTIONS
+
 vscode:
   extensions: # based on nf-core.nf-core-extensionpack
     - codezombiech.gitignore # Language support for .gitignore files
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 0c31cdb9..af57081f 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -1,5 +1,10 @@
 repos:
   - repo: https://github.com/pre-commit/mirrors-prettier
-    rev: "v2.7.1"
+    rev: "v3.1.0"
     hooks:
       - id: prettier
+  - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
+    rev: "2.7.3"
+    hooks:
+      - id: editorconfig-checker
+        alias: ec
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 809d1ec2..bca9b0c2 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.3dev - [date]
+## v2.3.0 - [date]
 
 Initial release of nf-core/smrnaseq, created with the [nf-core](https://nf-co.re/) template.
 
diff --git a/README.md b/README.md
index d0b5ad21..1095a9f3 100644
--- a/README.md
+++ b/README.md
@@ -1,5 +1,9 @@
-# ![nf-core/smrnaseq](docs/images/nf-core-smrnaseq_logo_light.png#gh-light-mode-only) ![nf-core/smrnaseq](docs/images/nf-core-smrnaseq_logo_dark.png#gh-dark-mode-only)
-
+<h1>
+  <picture>
+    <source media="(prefers-color-scheme: dark)" srcset="docs/images/nf-core-smrnaseq_logo_dark.png">
+    <img alt="nf-core/smrnaseq" src="docs/images/nf-core-smrnaseq_logo_light.png">
+  </picture>
+</h1>
 [![GitHub Actions CI Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/smrnaseq/actions?query=workflow%3A%22nf-core+CI%22)
 [![GitHub Actions Linting Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/smrnaseq/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/smrnaseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 
diff --git a/assets/email_template.html b/assets/email_template.html
index e75e86ac..d169743a 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -12,7 +12,7 @@
 
 <img src="cid:nfcorepipelinelogo">
 
-<h1>nf-core/smrnaseq v${version}</h1>
+<h1>nf-core/smrnaseq ${version}</h1>
 <h2>Run Name: $runName</h2>
 
 <% if (!success){
diff --git a/assets/email_template.txt b/assets/email_template.txt
index 654e78db..40a0e326 100644
--- a/assets/email_template.txt
+++ b/assets/email_template.txt
@@ -4,7 +4,7 @@
   |\\ | |__  __ /  ` /  \\ |__) |__         }  {
   | \\| |       \\__, \\__/ |  \\ |___     \\`-._,-`-,
                                         `._,._,'
-  nf-core/smrnaseq v${version}
+  nf-core/smrnaseq ${version}
 ----------------------------------------------------
 Run Name: $runName
 
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index b92cb9e7..77215840 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,7 +1,9 @@
 report_comment: >
-  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/tree/dev" target="_blank">nf-core/smrnaseq</a>
+  
+  This report has been generated by the <a href="https://github.com/nf-core/smrnaseq/releases/tag/2.3.0" target="_blank">nf-core/smrnaseq</a>
   analysis pipeline. For information about how to interpret these results, please see the
-  <a href="https://nf-co.re/smrnaseq/dev/docs/output" target="_blank">documentation</a>.
+  <a href="https://nf-co.re/smrnaseq/2.3.0/docs/output" target="_blank">documentation</a>.
+  
 report_section_order:
   "nf-core-smrnaseq-methods-description":
     order: -1000
diff --git a/assets/nf-core-smrnaseq_logo_light.png b/assets/nf-core-smrnaseq_logo_light.png
index 262fa1b099eb8b3cbd5f7d838f87a54dc0ec4ebe..9f8e05efd72fd5787d02c975aa05163e115632de 100644
GIT binary patch
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diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index 3b09f790..22deebd8 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -24,7 +24,7 @@ class WorkflowMain {
     //
     // Validate parameters and print summary to screen
     //
-    public static void initialise(workflow, params, log) {
+    public static void initialise(workflow, params, log, args) {
 
         // Print workflow version and exit on --version
         if (params.version) {
@@ -35,6 +35,8 @@ class WorkflowMain {
 
         // Check that a -profile or Nextflow config has been provided to run the pipeline
         NfcoreTemplate.checkConfigProvided(workflow, log)
+        // Check that the profile doesn't contain spaces and doesn't end with a trailing comma
+        checkProfile(workflow.profile, args, log)
 
         // Check that conda channels are set-up correctly
         if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
@@ -60,4 +62,16 @@ class WorkflowMain {
         }
         return null
     }
+
+    //
+    // Exit pipeline if --profile contains spaces
+    //
+    private static void checkProfile(profile, args, log) {
+        if (profile.endsWith(',')) {
+            Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`."
+        }
+        if (args[0]) {
+            log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n      Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`."
+        }
+    }
 }
diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar
deleted file mode 100644
index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000
GIT binary patch
literal 0
HcmV?d00001

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diff --git a/main.nf b/main.nf
index 6ec58e4d..8be7e198 100644
--- a/main.nf
+++ b/main.nf
@@ -44,7 +44,7 @@ if (params.validate_params) {
     validateParameters()
 }
 
-WorkflowMain.initialise(workflow, params, log)
+WorkflowMain.initialise(workflow, params, log, args)
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
diff --git a/modules.json b/modules.json
index 0606570e..f3d71e3b 100644
--- a/modules.json
+++ b/modules.json
@@ -7,17 +7,17 @@
                 "nf-core": {
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e",
+                        "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53",
+                        "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "4ab13872435962dadc239979554d13709e20bf29",
+                        "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
index f0c63f69..9b3272bc 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::multiqc=1.17
+  - bioconda::multiqc=1.19
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index 7685b33c..f2187611 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.17--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.19--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
index eec1db10..b1e1630b 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
+++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
@@ -31,7 +31,12 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                    process.out.versions,
+                    file(process.out.mqc_yml[0]).readLines()[0..10],
+                    file(process.out.yml[0]).readLines()[0..7]
+                    ).match()
+                }
             )
         }
     }
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
index 4274ed57..5f59a936 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
+++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
@@ -1,27 +1,33 @@
 {
     "Should run without failures": {
         "content": [
-            {
-                "0": [
-                    "software_versions.yml:md5,1c851188476409cda5752ce971b20b58"
-                ],
-                "1": [
-                    "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d"
-                ],
-                "2": [
-                    "versions.yml:md5,3843ac526e762117eedf8825b40683df"
-                ],
-                "mqc_yml": [
-                    "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d"
-                ],
-                "versions": [
-                    "versions.yml:md5,3843ac526e762117eedf8825b40683df"
-                ],
-                "yml": [
-                    "software_versions.yml:md5,1c851188476409cda5752ce971b20b58"
-                ]
-            }
+            [
+                "versions.yml:md5,76d454d92244589d32455833f7c1ba6d"
+            ],
+            [
+                "data: \"<style>\\n#nf-core-versions tbody:nth-child(even) {\\n    background-color: #f2f2f2;\\n\\",
+                "  }\\n</style>\\n<table class=\\\"table\\\" style=\\\"width:100%\\\" id=\\\"nf-core-versions\\\"\\",
+                "  >\\n    <thead>\\n        <tr>\\n            <th> Process Name </th>\\n            <th>\\",
+                "  \\ Software </th>\\n            <th> Version  </th>\\n        </tr>\\n    </thead>\\n\\",
+                "  \\n<tbody>\\n<tr>\\n    <td><samp>CUSTOM_DUMPSOFTWAREVERSIONS</samp></td>\\n    <td><samp>python</samp></td>\\n\\",
+                "  \\    <td><samp>3.11.7</samp></td>\\n</tr>\\n\\n<tr>\\n    <td><samp></samp></td>\\n \\",
+                "  \\   <td><samp>yaml</samp></td>\\n    <td><samp>5.4.1</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
+                "  <tbody>\\n<tr>\\n    <td><samp>TOOL1</samp></td>\\n    <td><samp>tool1</samp></td>\\n\\",
+                "  \\    <td><samp>0.11.9</samp></td>\\n</tr>\\n\\n</tbody>\\n<tbody>\\n<tr>\\n    <td><samp>TOOL2</samp></td>\\n\\",
+                "  \\    <td><samp>tool2</samp></td>\\n    <td><samp>1.9</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
+                "  <tbody>\\n<tr>\\n    <td><samp>Workflow</samp></td>\\n    <td><samp>Nextflow</samp></td>\\n\\"
+            ],
+            [
+                "CUSTOM_DUMPSOFTWAREVERSIONS:",
+                "  python: 3.11.7",
+                "  yaml: 5.4.1",
+                "TOOL1:",
+                "  tool1: 0.11.9",
+                "TOOL2:",
+                "  tool2: '1.9'",
+                "Workflow:"
+            ]
         ],
-        "timestamp": "2023-11-03T14:43:22.157011"
+        "timestamp": "2024-01-09T23:01:18.710682"
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
index b9e8f926..1f21c664 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -3,24 +3,20 @@ nextflow_process {
     name "Test Process FASTQC"
     script "../main.nf"
     process "FASTQC"
+
     tag "modules"
     tag "modules_nfcore"
     tag "fastqc"
 
-    test("Single-Read") {
+    test("sarscov2 single-end [fastq]") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id: 'test', single_end:true ],
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-                    ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
                 """
             }
         }
@@ -28,82 +24,189 @@ nextflow_process {
         then {
             assertAll (
             { assert process.success },
+
             // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
             // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
             // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
-            { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" },
-            { assert path(process.out.html.get(0).get(1)).getText().contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert snapshot(process.out.versions).match("versions") },
-            { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" }
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 interleaved [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true)
+                ])
+            """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
             )
         }
     }
-// TODO
-// //
-// // Test with paired-end data
-// //
-// workflow test_fastqc_paired_end {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 [
-//                     file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-//                 ]
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with interleaved data
-// //
-// workflow test_fastqc_interleaved {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true)
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with bam data
-// //
-// workflow test_fastqc_bam {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with multiple samples
-// //
-// workflow test_fastqc_multiple {
-//     input = [
-//                 [id: 'test', single_end: false], // meta map
-//                 [
-//                     file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true),
-//                     file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true)
-//                 ]
-//             ]
-
-//     FASTQC ( input )
-// }
-
-// //
-// // Test with custom prefix
-// //
-// workflow test_fastqc_custom_prefix {
-//     input = [
-//                 [ id:'mysample', single_end:true ], // meta map
-//                 file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-//             ]
-
-//     FASTQC ( input )
-// }
+
+    test("sarscov2 paired-end [bam]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 multiple [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+            { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" },
+            { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" },
+            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+            { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" },
+            { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" },
+            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 custom_prefix") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'mysample', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+
+            { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" },
+            { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
+            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+
+            { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastq] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id: 'test', single_end:true ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out.html.collect { file(it[1]).getName() } +
+                                process.out.zip.collect { file(it[1]).getName() } +
+                                process.out.versions ).match() }
+            )
+        }
+    }
+
 }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
index 636a32ce..5d624bb8 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test.snap
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -1,10 +1,20 @@
 {
+    "sarscov2 single-end [fastq] - stub": {
+        "content": [
+            [
+                "test.html",
+                "test.zip",
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "timestamp": "2024-01-17T18:40:57.254299"
+    },
     "versions": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
-        "timestamp": "2023-10-09T23:40:54+0000"
+        "timestamp": "2024-01-17T18:36:50.033627"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index bc0bdb5b..7625b752 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::multiqc=1.18
+  - bioconda::multiqc=1.19
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 00cc48d2..1b9f7c43 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.18--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.19--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
@@ -43,7 +43,7 @@ process MULTIQC {
 
     stub:
     """
-    touch multiqc_data
+    mkdir multiqc_data
     touch multiqc_plots
     touch multiqc_report.html
 
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index f1aa660e..45a9bc35 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,4 +1,3 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
 name: multiqc
 description: Aggregate results from bioinformatics analyses across many samples into a single report
 keywords:
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
index c2dad217..d0438eda 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -7,12 +7,9 @@ nextflow_process {
     tag "modules_nfcore"
     tag "multiqc"
 
-    test("MULTIQC: FASTQC") {
+    test("sarscov2 single-end [fastqc]") {
 
         when {
-            params {
-                outdir = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
@@ -26,20 +23,17 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert path(process.out.report.get(0)).exists() },
-                { assert path(process.out.data.get(0)).exists() },
-                { assert path(process.out.versions.get(0)).getText().contains("multiqc") }
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("versions") }
             )
         }
 
     }
 
-    test("MULTIQC: FASTQC and a config file") {
+    test("sarscov2 single-end [fastqc] [config]") {
 
         when {
-            params {
-                outdir = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
@@ -53,9 +47,35 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert path(process.out.report.get(0)).exists() },
-                { assert path(process.out.data.get(0)).exists() },
-                { assert path(process.out.versions.get(0)).getText().contains("multiqc") }
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastqc] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.report.collect { file(it).getName() } +
+                                process.out.data.collect { file(it).getName() } +
+                                process.out.plots.collect { file(it).getName() } +
+                                process.out.versions ).match() }
             )
         }
 
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
new file mode 100644
index 00000000..d37e7304
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -0,0 +1,21 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d"
+            ]
+        ],
+        "timestamp": "2024-01-09T23:02:49.911994"
+    },
+    "sarscov2 single-end [fastqc] - stub": {
+        "content": [
+            [
+                "multiqc_report.html",
+                "multiqc_data",
+                "multiqc_plots",
+                "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d"
+            ]
+        ],
+        "timestamp": "2024-01-09T23:03:14.524346"
+    }
+}
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index a473a3b6..02c8d3e2 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -71,7 +71,7 @@ try {
 }
 
 // Load nf-core/smrnaseq custom profiles from different institutions.
-// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs!
+// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs!
 // try {
 //   includeConfig "${params.custom_config_base}/pipeline/smrnaseq.config"
 // } catch (Exception e) {
@@ -91,6 +91,7 @@ profiles {
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        channels               = ['conda-forge', 'bioconda', 'defaults']
         apptainer.enabled      = false
     }
     mamba {
@@ -233,7 +234,7 @@ manifest {
     description     = """Small RNA-Seq Best Practice Analysis Pipeline."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '2.3dev'
+    version         = '2.3.0'
     doi             = ''
 }
 
diff --git a/pyproject.toml b/pyproject.toml
index 0d62beb6..7d08e1c8 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -1,10 +1,13 @@
-# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black.
+# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff.
 # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation.
-[tool.black]
+[tool.ruff]
 line-length = 120
-target_version = ["py37", "py38", "py39", "py310"]
+target-version = "py38"
+select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"]
+cache-dir = "~/.cache/ruff"
 
-[tool.isort]
-profile = "black"
-known_first_party = ["nf_core"]
-multi_line_output = 3
+[tool.ruff.isort]
+known-first-party = ["nf_core"]
+
+[tool.ruff.per-file-ignores]
+"__init__.py" = ["E402", "F401"]
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 17f366e3..95194afa 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -127,6 +127,13 @@ workflow.onComplete {
     }
 }
 
+workflow.onError {
+    if (workflow.errorReport.contains("Process requirement exceeds available memory")) {
+        println("🛑 Default resources exceed availability 🛑 ")
+        println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡")
+    }
+}
+
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     THE END

From bdbf992d89d49bf73bbb4660b99ddc29a60a82d8 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 07:56:22 +0000
Subject: [PATCH 133/227] Remove padding

---
 subworkflows/local/contaminant_filter.nf | 26 ++++++++++++------------
 1 file changed, 13 insertions(+), 13 deletions(-)

diff --git a/subworkflows/local/contaminant_filter.nf b/subworkflows/local/contaminant_filter.nf
index dede115d..02d89df7 100644
--- a/subworkflows/local/contaminant_filter.nf
+++ b/subworkflows/local/contaminant_filter.nf
@@ -3,23 +3,23 @@
 //
 
 include { BLAT_MIRNA as BLAT_CDNA
-          BLAT_MIRNA as BLAT_NCRNA
-          BLAT_MIRNA as BLAT_PIRNA
-          BLAT_MIRNA as BLAT_OTHER } from '../../modules/local/blat_mirna'
+        BLAT_MIRNA as BLAT_NCRNA
+        BLAT_MIRNA as BLAT_PIRNA
+        BLAT_MIRNA as BLAT_OTHER } from '../../modules/local/blat_mirna'
 
 include { INDEX_CONTAMINANTS as INDEX_RRNA
-          INDEX_CONTAMINANTS as INDEX_TRNA
-          INDEX_CONTAMINANTS as INDEX_CDNA
-          INDEX_CONTAMINANTS as INDEX_NCRNA
-          INDEX_CONTAMINANTS as INDEX_PIRNA
-          INDEX_CONTAMINANTS as INDEX_OTHER } from '../../modules/local/bowtie_contaminants'
+        INDEX_CONTAMINANTS as INDEX_TRNA
+        INDEX_CONTAMINANTS as INDEX_CDNA
+        INDEX_CONTAMINANTS as INDEX_NCRNA
+        INDEX_CONTAMINANTS as INDEX_PIRNA
+        INDEX_CONTAMINANTS as INDEX_OTHER } from '../../modules/local/bowtie_contaminants'
 
 include { BOWTIE_MAP_CONTAMINANTS as MAP_RRNA
-          BOWTIE_MAP_CONTAMINANTS as MAP_TRNA
-          BOWTIE_MAP_CONTAMINANTS as MAP_CDNA
-          BOWTIE_MAP_CONTAMINANTS as MAP_NCRNA
-          BOWTIE_MAP_CONTAMINANTS as MAP_PIRNA
-          BOWTIE_MAP_CONTAMINANTS as MAP_OTHER } from '../../modules/local/bowtie_map_contaminants'
+        BOWTIE_MAP_CONTAMINANTS as MAP_TRNA
+        BOWTIE_MAP_CONTAMINANTS as MAP_CDNA
+        BOWTIE_MAP_CONTAMINANTS as MAP_NCRNA
+        BOWTIE_MAP_CONTAMINANTS as MAP_PIRNA
+        BOWTIE_MAP_CONTAMINANTS as MAP_OTHER } from '../../modules/local/bowtie_map_contaminants'
 
 include { FILTER_STATS } from '../../modules/local/filter_stats'
 

From ab9157ebe6b441f0951b80c34216b32766ade45a Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 07:57:10 +0000
Subject: [PATCH 134/227] Updates

---
 modules.json                                  |  4 +-
 .../tests/main.nf.test                        | 28 ++++++-----
 .../tests/main.nf.test.snap                   |  6 +--
 .../bam_stats_samtools/tests/main.nf.test     | 48 +++++++++++--------
 .../tests/main.nf.test.snap                   |  6 +--
 5 files changed, 51 insertions(+), 41 deletions(-)

diff --git a/modules.json b/modules.json
index 11ca6940..680617e8 100644
--- a/modules.json
+++ b/modules.json
@@ -81,12 +81,12 @@
                 "nf-core": {
                     "bam_sort_stats_samtools": {
                         "branch": "master",
-                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647",
                         "installed_by": ["subworkflows"]
                     },
                     "bam_stats_samtools": {
                         "branch": "master",
-                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647",
                         "installed_by": ["bam_sort_stats_samtools", "subworkflows"]
                     },
                     "fastq_fastqc_umitools_fastp": {
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test
index 59b749d8..75b5b934 100644
--- a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test
@@ -24,12 +24,14 @@ nextflow_workflow {
             }
             workflow {
                 """
-                input[0] = [ [ id:'test', single_end:false ], // meta map
-                        file(params.test_data['sarscov2']['illumina']['test_single_end_bam'], checkIfExists: true)
-                        ]
-                input[1] = [ [ id:'genome' ],
-                        file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
-                        ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -54,12 +56,14 @@ nextflow_workflow {
             }
             workflow {
                 """
-                input[0] = [ [ id:'test', single_end:false ], // meta map
-                        file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true)
-                        ]
-                input[1] = [ [ id:'genome' ],
-                        file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
-                        ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
                 """
             }
         }
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
index 77afbf17..c159eef3 100644
--- a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
@@ -39,7 +39,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-12-04T11:06:50.951881479"
+        "timestamp": "2024-01-18T17:10:02.818694"
     },
     "test_bam_sort_stats_samtools_paired_end_stats": {
         "content": [
@@ -67,7 +67,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-10-22T20:25:58.451364604"
+        "timestamp": "2024-01-18T17:10:02.84631"
     },
     "test_bam_sort_stats_samtools_single_end_flagstats": {
         "content": [
@@ -81,6 +81,6 @@
                 ]
             ]
         ],
-        "timestamp": "2023-10-22T20:25:58.416859285"
+        "timestamp": "2024-01-18T17:10:02.829756"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
index 97210890..c8b21f28 100644
--- a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
@@ -20,13 +20,15 @@ nextflow_workflow {
             }
             workflow {
                 """
-                    input[0] = [ [ id:'test', single_end:true ], // meta map
-                                file(params.test_data['sarscov2']['illumina']['test_single_end_sorted_bam'], checkIfExists: true),
-                                file(params.test_data['sarscov2']['illumina']['test_single_end_sorted_bam_bai'], checkIfExists: true)
-                        ]
-                    input[1] = [ [ id:'genome' ],
-                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
-                            ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -49,13 +51,15 @@ nextflow_workflow {
             }
             workflow {
                 """
-                    input[0] = [ [ id:'test', single_end:true ], // meta map
-                                file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
-                                file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
-                        ]
-                    input[1] = [ [ id:'genome' ],
-                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
-                            ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -78,13 +82,15 @@ nextflow_workflow {
             }
             workflow {
                 """
-                     input[0] = [ [ id:'test', single_end:false ], // meta map
-                                file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true),
-                                file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram_crai'], checkIfExists: true)
-                                ]
-                    input[1] = [ [ id:'genome' ],
-                            file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-                            ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
                 """
             }
         }
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
index d3af1376..8bf0d379 100644
--- a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
@@ -25,7 +25,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-12-04T11:07:13.965061942"
+        "timestamp": "2024-01-18T17:17:27.704335"
     },
     "test_bam_stats_samtools_paired_end_flagstats": {
         "content": [
@@ -39,7 +39,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-06T09:31:11.668517251"
+        "timestamp": "2024-01-18T17:17:27.717482"
     },
     "test_bam_stats_samtools_single_end_flagstats": {
         "content": [
@@ -95,7 +95,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-06T09:31:11.68246157"
+        "timestamp": "2024-01-18T17:17:27.726719"
     },
     "test_bam_stats_samtools_single_end_idxstats": {
         "content": [

From 27ca21b3dfbe433dbbc89f2662225c9b5b47560b Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 08:07:19 +0000
Subject: [PATCH 135/227] Lets see

---
 .devcontainer/devcontainer.json         | 8 ++++----
 .github/workflows/download_pipeline.yml | 2 +-
 nextflow_schema.json                    | 3 +++
 3 files changed, 8 insertions(+), 5 deletions(-)

diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json
index 4a9bc5c7..4ecfbfe3 100644
--- a/.devcontainer/devcontainer.json
+++ b/.devcontainer/devcontainer.json
@@ -18,11 +18,11 @@
                 "python.linting.flake8Path": "/opt/conda/bin/flake8",
                 "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle",
                 "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle",
-                "python.linting.pylintPath": "/opt/conda/bin/pylint",
+                "python.linting.pylintPath": "/opt/conda/bin/pylint"
             },
 
             // Add the IDs of extensions you want installed when the container is created.
-            "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"],
-        },
-    },
+            "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"]
+        }
+    }
 }
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 8611458a..8a330045 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -64,4 +64,4 @@ jobs:
         env:
           NXF_SINGULARITY_CACHEDIR: ./
           NXF_SINGULARITY_HOME_MOUNT: true
-        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results 
+        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 204376ab..368e85ac 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -239,6 +239,9 @@
                 },
                 "fastp_known_mirna_adapters": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "mimetype": "text/plain",
                     "default": "${projectDir}/assets/known_adapters.fa",
                     "description": "FastA with known miRNA adapter sequences for adapter trimming",
                     "fa_icon": "far fa-question-circle"

From 79b44f6f145167865c2bd11960ae48b440533a3c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 08:21:11 +0000
Subject: [PATCH 136/227] Exclude mirna adapters from lint test

---
 .nf-core.yml | 4 ++++
 1 file changed, 4 insertions(+)

diff --git a/.nf-core.yml b/.nf-core.yml
index 3805dc81..8a74623b 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1 +1,5 @@
 repository_type: pipeline
+lint:
+  nextflow_config:
+    - config_defaults:
+        - params.fastp_known_mirna_adapters

From 9266535ca198553b98851f78f10b58a5127ccce8 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 08:30:10 +0000
Subject: [PATCH 137/227] Changelog++, umicollapse update

---
 CHANGELOG.md                                  |  1 +
 modules.json                                  |  2 +-
 modules/nf-core/umicollapse/main.nf           | 22 +++++++++----
 modules/nf-core/umicollapse/meta.yml          | 10 +++++-
 .../nf-core/umicollapse/tests/main.nf.test    | 32 ++++++++++++++++++-
 .../umicollapse/tests/main.nf.test.snap       | 31 +++++++++++++++---
 6 files changed, 85 insertions(+), 13 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index be1d0138..18232b5f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [[#164]](https://github.com/nf-core/smrnaseq/pull/164) - UMI Handling Feature implemented in the pipeline
 - [[#302]](https://github.com/nf-core/smrnaseq/pull/302) - Merged in nf-core template v2.11.1
 - [[#294]](https://github.com/nf-core/smrnaseq/pull/294) - Fixed contamination screening issues
+- [[#309]](https://github.com/nf-core/smrnaseq/pull/309) - Merged in nf-core template v2.12.0
 
 ### Parameters
 
diff --git a/modules.json b/modules.json
index 680617e8..9cc6b1e2 100644
--- a/modules.json
+++ b/modules.json
@@ -67,7 +67,7 @@
                     },
                     "umicollapse": {
                         "branch": "master",
-                        "git_sha": "b573d74ce7eced7963d00d4a7f99db9caed61d79",
+                        "git_sha": "0f0531e44e5d106fc7ef4aadfb23352b09da80a9",
                         "installed_by": ["modules"]
                     },
                     "umitools/extract": {
diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 15109077..b5bc96c5 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -9,10 +9,12 @@ process UMICOLLAPSE {
         'biocontainers/umicollapse:1.0.0--hdfd78af_1' }"
 
     input:
-    tuple val(meta), path(bam), path(bai)
+    tuple val(meta), path(input), path(bai)
+    val(mode)
 
     output:
-    tuple val(meta), path("*.bam"), emit: bam
+    tuple val(meta), path("*.bam"), emit: bam,             optional: true
+    tuple val(meta), path("*.fastq.gz"), emit: fastq,      optional: true
     tuple val(meta), path("*_UMICollapse.log"), emit: log
     path  "versions.yml"          , emit: versions
 
@@ -28,6 +30,10 @@ process UMICOLLAPSE {
     // which leaves 5% for stuff happening outside of java without the scheduler killing the process.
     def max_heap_size_mega = (task.memory.toMega() * 0.9).intValue()
     def max_stack_size_mega = (task.memory.toMega() * 0.05).intValue()
+    if ( mode !in [ 'fastq', 'bam' ] ) {
+        error "Mode must be one of 'fastq' or 'bam'."
+    }
+    extension = mode.contains("fastq") ? "fastq.gz" : "bam"
     """
     # Getting the umicollapse jar file like this because `umicollapse` is a Python wrapper script generated
     # by conda that allows to set the heap size (Xmx), but not the stack size (Xss).
@@ -38,9 +44,9 @@ process UMICOLLAPSE {
         -Xmx${max_heap_size_mega}M \\
         -Xss${max_stack_size_mega}M \\
         -jar \$UMICOLLAPSE_JAR \\
-        bam \\
-        -i $bam \\
-        -o ${prefix}.bam \\
+        $mode \\
+        -i ${input} \\
+        -o ${prefix}.${extension} \\
         $args | tee ${prefix}_UMICollapse.log
 
     cat <<-END_VERSIONS > versions.yml
@@ -52,8 +58,12 @@ process UMICOLLAPSE {
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     def VERSION = '1.0.0-1'
+    if ( mode !in [ 'fastq', 'bam' ] ) {
+        error "Mode must be one of 'fastq' or 'bam'."
+    }
+    extension = mode.contains("fastq") ? "fastq.gz" : "bam"
     """
-    touch ${prefix}.dedup.bam
+    touch ${prefix}.dedup.${extension}
     touch ${prefix}_UMICollapse.log
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/umicollapse/meta.yml b/modules/nf-core/umicollapse/meta.yml
index c45d6932..c1361f9a 100644
--- a/modules/nf-core/umicollapse/meta.yml
+++ b/modules/nf-core/umicollapse/meta.yml
@@ -27,8 +27,14 @@ input:
   - bai:
       type: file
       description: |
-        BAM index files corresponding to the input BAM file.
+        BAM index files corresponding to the input BAM file. Optionally can be skipped using [] when using FastQ input.
       pattern: "*.{bai}"
+  - mode:
+      type: string
+      description: |
+        Selects the mode of Umicollapse - either fastq or bam need to be provided.
+      pattern: "{fastq,bam}"
+
 output:
   - meta:
       type: map
@@ -53,3 +59,5 @@ authors:
 maintainers:
   - "@CharlotteAnne"
   - "@chris-cheshire"
+  - "@apeltzer"
+  - "@MatthiasZepper"
diff --git a/modules/nf-core/umicollapse/tests/main.nf.test b/modules/nf-core/umicollapse/tests/main.nf.test
index 27c3e7a1..36cd748b 100644
--- a/modules/nf-core/umicollapse/tests/main.nf.test
+++ b/modules/nf-core/umicollapse/tests/main.nf.test
@@ -66,6 +66,7 @@ nextflow_process {
             process {
                 """
                 input[0] =  BWA_MEM.out.bam.join(SAMTOOLS_INDEX.out.bai, by: [0])
+                input[1] = 'bam'
                 """
             }
         }
@@ -135,6 +136,7 @@ nextflow_process {
             process {
                 """
                 input[0] =  BWA_MEM.out.bam.join(SAMTOOLS_INDEX.out.bai, by: [0])
+                input[1] = 'bam'
                 """
             }
         }
@@ -150,6 +152,32 @@ nextflow_process {
 
     }
 
+    test("umicollapse fastq tests") {
+
+            when {
+                config "./nextflow_SE.config"
+                process {
+                    """
+                    input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
+                    []
+                    ]
+                    input[1] = 'fastq'
+                    """
+                }
+            }
+
+            then {
+                assertAll(
+                    { assert process.success },
+                    { assert snapshot(
+                        process.out.fastq,
+                        process.out.versions).match() }
+                )
+            }
+    }
+
     test("umicollapse stub tests") {
         options "-stub-run"
             setup{
@@ -204,6 +232,7 @@ nextflow_process {
             process {
                 """
                 input[0] =  BWA_MEM.out.bam.join(SAMTOOLS_INDEX.out.bai, by: [0])
+                input[1] = 'bam'
                 """
             }
         }
@@ -216,4 +245,5 @@ nextflow_process {
         }
 
     }
-}
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/umicollapse/tests/main.nf.test.snap b/modules/nf-core/umicollapse/tests/main.nf.test.snap
index 8cc979aa..5cee4d4c 100644
--- a/modules/nf-core/umicollapse/tests/main.nf.test.snap
+++ b/modules/nf-core/umicollapse/tests/main.nf.test.snap
@@ -14,7 +14,24 @@
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
-        "timestamp": "2024-01-24T21:34:08.702681823"
+        "timestamp": "2024-01-30T07:40:40.750602889"
+    },
+    "umicollapse fastq tests": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.dedup.fastq.gz:md5,c9bac08c7fd8df3e0203e3eeafc73155"
+                ]
+            ],
+            [
+                "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
+            ]
+        ],
+        "timestamp": "2024-01-30T07:41:28.990304042"
     },
     "umicollapse stub tests": {
         "content": [
@@ -29,6 +46,9 @@
                     ]
                 ],
                 "1": [
+                    
+                ],
+                "2": [
                     [
                         {
                             "id": "test",
@@ -37,7 +57,7 @@
                         "test.dedup_UMICollapse.log:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "2": [
+                "3": [
                     "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
                 ],
                 "bam": [
@@ -48,6 +68,9 @@
                         },
                         "test.dedup.dedup.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
+                ],
+                "fastq": [
+                    
                 ],
                 "log": [
                     [
@@ -63,7 +86,7 @@
                 ]
             }
         ],
-        "timestamp": "2024-01-24T21:34:48.437479265"
+        "timestamp": "2024-01-30T07:41:55.110612801"
     },
     "umicollapse paired tests": {
         "content": [
@@ -80,6 +103,6 @@
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
-        "timestamp": "2024-01-24T21:34:25.444373007"
+        "timestamp": "2024-01-30T07:41:07.399188554"
     }
 }
\ No newline at end of file

From 8e73796c2dcaeb095a0db69d1b1fcf23cb01d171 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 08:50:23 +0000
Subject: [PATCH 138/227] Cleaning up UMI dedp to fastq based

---
 conf/modules.config                           |  2 +-
 modules.json                                  |  5 --
 .../nf-core/samtools/bam2fq/environment.yml   |  7 --
 modules/nf-core/samtools/bam2fq/main.nf       | 56 ---------------
 modules/nf-core/samtools/bam2fq/meta.yml      | 51 -------------
 .../samtools/bam2fq/tests/main.nf.test        | 71 -------------------
 .../samtools/bam2fq/tests/main.nf.test.snap   | 49 -------------
 .../samtools/bam2fq/tests/nextflow.config     |  3 -
 .../nf-core/samtools/bam2fq/tests/tags.yml    |  2 -
 modules/nf-core/umicollapse/main.nf           |  2 +-
 subworkflows/local/umi_dedup.nf               | 46 ------------
 workflows/smrnaseq.nf                         | 36 +++++-----
 12 files changed, 21 insertions(+), 309 deletions(-)
 delete mode 100644 modules/nf-core/samtools/bam2fq/environment.yml
 delete mode 100644 modules/nf-core/samtools/bam2fq/main.nf
 delete mode 100644 modules/nf-core/samtools/bam2fq/meta.yml
 delete mode 100644 modules/nf-core/samtools/bam2fq/tests/main.nf.test
 delete mode 100644 modules/nf-core/samtools/bam2fq/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/samtools/bam2fq/tests/nextflow.config
 delete mode 100644 modules/nf-core/samtools/bam2fq/tests/tags.yml
 delete mode 100644 subworkflows/local/umi_dedup.nf

diff --git a/conf/modules.config b/conf/modules.config
index cbd7d5d1..01ccd295 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -165,7 +165,7 @@ process {
             pattern: '*.{stats,flagstat,idxstats}'
         ]
     }
-    withName: '.*:DEDUPLICATE_UMIS:UMICOLLAPSE' {
+    withName: '.*:UMICOLLAPSE' {
         ext.args = { meta.single_end ? "--algo ${params.umitools_method} --two-pass" : "--method ${params.umitools_method} --two-pass --paired --remove-unpaired --remove-chimeric" }
         ext.prefix = { "${meta.id}.umi_dedup.sorted" }
         publishDir = [
diff --git a/modules.json b/modules.json
index 9cc6b1e2..8cb6236a 100644
--- a/modules.json
+++ b/modules.json
@@ -35,11 +35,6 @@
                         "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
                         "installed_by": ["modules"]
                     },
-                    "samtools/bam2fq": {
-                        "branch": "master",
-                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
-                        "installed_by": ["modules"]
-                    },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
diff --git a/modules/nf-core/samtools/bam2fq/environment.yml b/modules/nf-core/samtools/bam2fq/environment.yml
deleted file mode 100644
index 5297496f..00000000
--- a/modules/nf-core/samtools/bam2fq/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-name: samtools_bam2fq
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::samtools=1.18
diff --git a/modules/nf-core/samtools/bam2fq/main.nf b/modules/nf-core/samtools/bam2fq/main.nf
deleted file mode 100644
index 55ffd0cf..00000000
--- a/modules/nf-core/samtools/bam2fq/main.nf
+++ /dev/null
@@ -1,56 +0,0 @@
-process SAMTOOLS_BAM2FQ {
-    tag "$meta.id"
-    label 'process_low'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
-        'biocontainers/samtools:1.18--h50ea8bc_1' }"
-
-    input:
-    tuple val(meta), path(inputbam)
-    val split
-
-    output:
-    tuple val(meta), path("*.fq.gz"), emit: reads
-    path "versions.yml"             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-
-    if (split){
-        """
-        samtools \\
-            bam2fq \\
-            $args \\
-            -@ $task.cpus \\
-            -1 ${prefix}_1.fq.gz \\
-            -2 ${prefix}_2.fq.gz \\
-            -0 ${prefix}_other.fq.gz \\
-            -s ${prefix}_singleton.fq.gz \\
-            $inputbam
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-        END_VERSIONS
-        """
-    } else {
-        """
-        samtools \\
-            bam2fq \\
-            $args \\
-            -@ $task.cpus \\
-            $inputbam | gzip --no-name > ${prefix}_interleaved.fq.gz
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-        END_VERSIONS
-        """
-    }
-}
diff --git a/modules/nf-core/samtools/bam2fq/meta.yml b/modules/nf-core/samtools/bam2fq/meta.yml
deleted file mode 100644
index 7769046b..00000000
--- a/modules/nf-core/samtools/bam2fq/meta.yml
+++ /dev/null
@@ -1,51 +0,0 @@
-name: samtools_bam2fq
-description: |
-  The module uses bam2fq method from samtools to
-  convert a SAM, BAM or CRAM file to FASTQ format
-keywords:
-  - bam2fq
-  - samtools
-  - fastq
-tools:
-  - samtools:
-      description: Tools for dealing with SAM, BAM and CRAM files
-      documentation: http://www.htslib.org/doc/1.1/samtools.html
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - inputbam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - split:
-      type: boolean
-      description: |
-        TRUE/FALSE value to indicate if reads should be separated into
-        /1, /2 and if present other, or singleton.
-        Note: choosing TRUE will generate 4 different files.
-        Choosing FALSE will produce a single file, which will be interleaved in case
-        the input contains paired reads.
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - reads:
-      type: file
-      description: |
-        FASTQ files, which will be either a group of 4 files (read_1, read_2, other and singleton)
-        or a single interleaved .fq.gz file if the user chooses not to split the reads.
-      pattern: "*.fq.gz"
-authors:
-  - "@lescai"
-maintainers:
-  - "@lescai"
diff --git a/modules/nf-core/samtools/bam2fq/tests/main.nf.test b/modules/nf-core/samtools/bam2fq/tests/main.nf.test
deleted file mode 100644
index cd65abbe..00000000
--- a/modules/nf-core/samtools/bam2fq/tests/main.nf.test
+++ /dev/null
@@ -1,71 +0,0 @@
-nextflow_process {
-
-    name "Test Process SAMTOOLS_BAM2FQ"
-    script "../main.nf"
-    process "SAMTOOLS_BAM2FQ"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "samtools"
-    tag "samtools/bam2fq"
-
-    config "./nextflow.config"
-
-    test("homo_sapiens - bam, false") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                    file(params.test_data['homo_sapiens']['illumina']['test_paired_end_umi_converted_bam'], checkIfExists: true)
-                ]
-                input[1] = false
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    path(process.out.reads[0][1]).linesGzip[0..6],
-                    process.out.versions
-                ).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - bam, true") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                    file(params.test_data['homo_sapiens']['illumina']['test_paired_end_umi_converted_bam'], checkIfExists: true)
-                ]
-                input[1] = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.reads[0][1].collect{
-                        if(it ==~ /.*(other|singleton)\.fq\.gz$/) {
-                            return file(it).name
-                        }
-                        return path(it).linesGzip[0..6]
-                    },
-                    process.out.versions
-                ).match() }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/samtools/bam2fq/tests/main.nf.test.snap b/modules/nf-core/samtools/bam2fq/tests/main.nf.test.snap
deleted file mode 100644
index 1f824503..00000000
--- a/modules/nf-core/samtools/bam2fq/tests/main.nf.test.snap
+++ /dev/null
@@ -1,49 +0,0 @@
-{
-    "homo_sapiens - bam, false": {
-        "content": [
-            [
-                "@922332/1\tRX:Z:ATTTCAG-TATTATT",
-                "GAGAGGATCTCGTGTAGAAATTGCTTTGAGCTGTTCTTTGTCATTTTCCCTTAATTCATTGTCTCTAGCTAGTCTGTTACTCTGTAAAATAAAATAATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTG",
-                "+",
-                "EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEEAEAEE6AAEEEEE/EAAAA<AEEEEAAEEAAAA<EEE/",
-                "@922332/2\tRX:Z:ATTTCAG-TATTATT",
-                "TATTTTACAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTCTCTGAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGAT",
-                "+"
-            ],
-            [
-                "versions.yml:md5,0b88ba6888f9730c167242c0c4b3d04b"
-            ]
-        ],
-        "timestamp": "2023-12-04T13:40:52.124056991"
-    },
-    "homo_sapiens - bam, true": {
-        "content": [
-            [
-                [
-                    "@922332\tRX:Z:ATTTCAG-TATTATT",
-                    "GAGAGGATCTCGTGTAGAAATTGCTTTGAGCTGTTCTTTGTCATTTTCCCTTAATTCATTGTCTCTAGCTAGTCTGTTACTCTGTAAAATAAAATAATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTG",
-                    "+",
-                    "EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEEAEAEE6AAEEEEE/EAAAA<AEEEEAAEEAAAA<EEE/",
-                    "@928177\tRX:Z:ACATAAA-TGAGATT",
-                    "AAAAGTATATAAGTAATACATATTTATAAATCTATTAAGAAAGCAAGTAATATGTACCTTAAGAATTTAATGGGAAAATAATTAGACTTACTTTAAATGCCAAAAGAAAAAGTGCCCAATCCTTTGATTAGTCAATGCTTTCT",
-                    "+"
-                ],
-                [
-                    "@922332\tRX:Z:ATTTCAG-TATTATT",
-                    "TATTTTACAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTCTCTGAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGAT",
-                    "+",
-                    "EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEA<AEEE<<<<AAEEEEEEEEEEA<EEEAEAE//A<AAE<6",
-                    "@928177\tRX:Z:ACATAAA-TGAGATT",
-                    "TTACTGAAGAAAGCATTGACTAATCAAAGGATTGGGCACTTTTTCTTTTGGCATTTAAAGTAAGTCTAATTATTTTCCCATTAAATTCTTAAGGTACATATTACTTGCTTTCTTAATAGATTTATAAATATGTATTACTTATA",
-                    "+"
-                ],
-                "test_other.fq.gz",
-                "test_singleton.fq.gz"
-            ],
-            [
-                "versions.yml:md5,0b88ba6888f9730c167242c0c4b3d04b"
-            ]
-        ],
-        "timestamp": "2023-12-04T13:45:59.949456034"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/bam2fq/tests/nextflow.config b/modules/nf-core/samtools/bam2fq/tests/nextflow.config
deleted file mode 100644
index dae4e48b..00000000
--- a/modules/nf-core/samtools/bam2fq/tests/nextflow.config
+++ /dev/null
@@ -1,3 +0,0 @@
-process {
-    ext.args = '-T RX'
-}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/bam2fq/tests/tags.yml b/modules/nf-core/samtools/bam2fq/tests/tags.yml
deleted file mode 100644
index 2fbf4a1f..00000000
--- a/modules/nf-core/samtools/bam2fq/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-samtools/bam2fq:
-  - "modules/nf-core/samtools/bam2fq/**"
diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index b5bc96c5..133a4d45 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -14,7 +14,7 @@ process UMICOLLAPSE {
 
     output:
     tuple val(meta), path("*.bam"), emit: bam,             optional: true
-    tuple val(meta), path("*.fastq.gz"), emit: fastq,      optional: true
+    tuple val(meta), path("*${prefix}.fastq.gz"), emit: fastq,      optional: true
     tuple val(meta), path("*_UMICollapse.log"), emit: log
     path  "versions.yml"          , emit: versions
 
diff --git a/subworkflows/local/umi_dedup.nf b/subworkflows/local/umi_dedup.nf
deleted file mode 100644
index 2c7b533b..00000000
--- a/subworkflows/local/umi_dedup.nf
+++ /dev/null
@@ -1,46 +0,0 @@
-//
-// Deduplicate the UMI reads by mapping them to the complete genome.
-//
-
-include { INDEX_GENOME                        } from '../../modules/local/bowtie_genome'
-include { BOWTIE_MAP_SEQ as UMI_MAP_GENOME    } from '../../modules/local/bowtie_map_mirna'
-include { BAM_SORT_STATS_SAMTOOLS             } from '../../subworkflows/nf-core/bam_sort_stats_samtools'
-include { UMICOLLAPSE                         } from '../../modules/nf-core/umicollapse/main'
-include { SAMTOOLS_BAM2FQ                     } from '../../modules/nf-core/samtools/bam2fq/main'
-include { CAT_CAT                             } from '../../modules/nf-core/cat/cat/main'
-include { FASTQC as FASTQC_DEDUPLICATED        } from '../../modules/nf-core/fastqc/main'
-
-
-workflow DEDUPLICATE_UMIS {
-    take:
-    bt_index
-    reads      // channel: [ val(meta), [ reads ] ]
-
-    main:
-
-    ch_versions = Channel.empty()
-
-    UMI_MAP_GENOME ( reads, bt_index.collect() )
-    ch_versions = ch_versions.mix(UMI_MAP_GENOME.out.versions)
-
-    BAM_SORT_STATS_SAMTOOLS ( UMI_MAP_GENOME.out.bam, Channel.empty() )
-    ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions)
-
-    ch_umi_dedup = BAM_SORT_STATS_SAMTOOLS.out.bam.join(BAM_SORT_STATS_SAMTOOLS.out.bai)
-    UMICOLLAPSE(ch_umi_dedup)
-    ch_versions = ch_versions.mix(UMICOLLAPSE.out.versions)
-
-    SAMTOOLS_BAM2FQ ( UMICOLLAPSE.out.bam, false )
-    ch_versions = ch_versions.mix(SAMTOOLS_BAM2FQ.out.versions)
-
-    ch_dedup_reads = SAMTOOLS_BAM2FQ.out.reads
-
-    FASTQC_DEDUPLICATED(ch_dedup_reads)
-
-    emit:
-    reads = ch_dedup_reads
-    fastqc_html = FASTQC_DEDUPLICATED.out.html
-    fastqc_zip = FASTQC_DEDUPLICATED.out.zip
-    indices  = bt_index
-    versions = ch_versions
-}
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 43fbcfa0..514939d3 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -66,7 +66,6 @@ if (!params.mirgenedb) {
 
 include { INPUT_CHECK                 } from '../subworkflows/local/input_check'
 include { FASTQ_FASTQC_UMITOOLS_FASTP } from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp'
-include { DEDUPLICATE_UMIS            } from '../subworkflows/local/umi_dedup'
 include { CONTAMINANT_FILTER          } from '../subworkflows/local/contaminant_filter'
 include { MIRNA_QUANT                 } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT                } from '../subworkflows/local/genome_quant'
@@ -83,10 +82,10 @@ include { INDEX_GENOME                } from '../modules/local/bowtie_genome'
 //
 // MODULE: Installed directly from nf-core/modules
 //
-include { CAT_FASTQ                   } from '../modules/nf-core/cat/fastq/main'
-include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
-include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
-
+include { CAT_FASTQ                        } from '../modules/nf-core/cat/fastq/main'
+include { MULTIQC                          } from '../modules/nf-core/multiqc/main'
+include { CUSTOM_DUMPSOFTWAREVERSIONS      } from '../modules/nf-core/custom/dumpsoftwareversions/main'
+include { UMICOLLAPSE as UMICOLLAPSE_FASTQ } from '../modules/nf-core/umicollapse/main'
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -150,9 +149,6 @@ workflow SMRNASEQ {
     )
     ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
 
-    if(params.with_umi && !params.fasta) {
-        error "Specifying a genome fasta is required for UMI deduplication"
-    }
     ch_fasta = params.fasta ? file(params.fasta): []
     ch_reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
 
@@ -160,7 +156,7 @@ workflow SMRNASEQ {
     // without fasta, no genome analysis.
     if(params.fasta) {
         //Prepare bowtie index, unless specified
-        //This needs to be done here as the index is used by both UMI deduplication and GENOME_QUANT
+        //This needs to be done here as the index is used by GENOME_QUANT
         if(params.bowtie_index) {
             ch_bowtie_index  = Channel.fromPath("${index}**ebwt", checkIfExists: true).ifEmpty { error "Bowtie1 index directory not found: ${index}" }
         } else {
@@ -175,13 +171,19 @@ workflow SMRNASEQ {
     //
     // SUBWORKFLOW: Deduplicate UMIs by mapping them to the genome
     //
-    if (params.with_umi){
-        DEDUPLICATE_UMIS (
-            ch_bowtie_index,
-            ch_reads_for_mirna,
-        )
-        ch_reads_for_mirna = DEDUPLICATE_UMIS.out.reads
-        ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)
+    // if (params.with_umi){
+    //     DEDUPLICATE_UMIS (
+    //         ch_bowtie_index,
+    //         ch_reads_for_mirna,
+    //     )
+    //     ch_reads_for_mirna = DEDUPLICATE_UMIS.out.reads
+    //     ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)
+    // }
+
+    //UMI Dedup for fastq input
+    if (params.with_umi) {
+        UMICOLLAPSE_FASTQ(ch_reads_for_mirna, 'fastq')
+        ch_reads_for_mirna = UMICOLLAPSE_FASTQ.out.fastq
     }
 
 
@@ -275,7 +277,7 @@ workflow SMRNASEQ {
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_json.collect{it[1]}.ifEmpty([]))
         if(params.with_umi) {
-            ch_multiqc_files = ch_multiqc_files.mix(DEDUPLICATE_UMIS.out.fastqc_zip.collect{it[1]}.ifEmpty([]))
+            ch_multiqc_files = ch_multiqc_files.mix(UMICOLLAPSE_FASTQ.out.log.collect().ifEmpty([]))
         }
         ch_multiqc_files = ch_multiqc_files.mix(contamination_stats.collect().ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(genome_stats.collect({it[1]}).ifEmpty([]))

From 7c46a1e5cb09e9a3cda7a92157bca59e84f8b67f Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 09:21:33 +0000
Subject: [PATCH 139/227] Channel magic

---
 workflows/smrnaseq.nf | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 514939d3..077fef9d 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -182,7 +182,9 @@ workflow SMRNASEQ {
 
     //UMI Dedup for fastq input
     if (params.with_umi) {
-        UMICOLLAPSE_FASTQ(ch_reads_for_mirna, 'fastq')
+        ch_fastq = Channel.value('fastq')
+        ch_input_for_collapse = ch_reads_for_mirna.map{ meta, reads -> [meta, reads, []]} //Needs to be done to add a []
+        UMICOLLAPSE_FASTQ(ch_input_for_collapse, ch_fastq)
         ch_reads_for_mirna = UMICOLLAPSE_FASTQ.out.fastq
     }
 

From 7aba903444f126a93c83ae86d82c0248122b0247 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 09:28:01 +0000
Subject: [PATCH 140/227] Fix MultiQC

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 077fef9d..cb589d09 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -279,7 +279,7 @@ workflow SMRNASEQ {
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_json.collect{it[1]}.ifEmpty([]))
         if(params.with_umi) {
-            ch_multiqc_files = ch_multiqc_files.mix(UMICOLLAPSE_FASTQ.out.log.collect().ifEmpty([]))
+            ch_multiqc_files = ch_multiqc_files.mix(UMICOLLAPSE_FASTQ.out.log.collect{it[1]}.ifEmpty([]))
         }
         ch_multiqc_files = ch_multiqc_files.mix(contamination_stats.collect().ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(genome_stats.collect({it[1]}).ifEmpty([]))

From c462d0742730655fe3611695d7794d6c0ac17958 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 10:00:01 +0000
Subject: [PATCH 141/227] Getting more stuff in

---
 conf/modules.config                 |  4 ++--
 modules/nf-core/umicollapse/main.nf |  4 ++--
 workflows/smrnaseq.nf               | 13 +------------
 3 files changed, 5 insertions(+), 16 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 01ccd295..3cebdc02 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -165,13 +165,13 @@ process {
             pattern: '*.{stats,flagstat,idxstats}'
         ]
     }
-    withName: '.*:UMICOLLAPSE' {
+    withName: '.*:UMICOLLAPSE_FASTQ' {
         ext.args = { meta.single_end ? "--algo ${params.umitools_method} --two-pass" : "--method ${params.umitools_method} --two-pass --paired --remove-unpaired --remove-chimeric" }
         ext.prefix = { "${meta.id}.umi_dedup.sorted" }
         publishDir = [
             path: { "${params.outdir}/umi_dedup/bam_deduplicated" },
             mode: params.publish_dir_mode,
-            pattern: '*.bam',
+            pattern: '*.{bam,fastq.gz}',
             enabled: (
                 params.save_umi_intermeds
             )
diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 133a4d45..321ffd84 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -13,8 +13,8 @@ process UMICOLLAPSE {
     val(mode)
 
     output:
-    tuple val(meta), path("*.bam"), emit: bam,             optional: true
-    tuple val(meta), path("*${prefix}.fastq.gz"), emit: fastq,      optional: true
+    tuple val(meta), path("*.bam"), emit: bam,                         optional: true
+    tuple val(meta), path("${prefix}.${extension}"), emit: fastq,      optional: true
     tuple val(meta), path("*_UMICollapse.log"), emit: log
     path  "versions.yml"          , emit: versions
 
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index cb589d09..f84b8f86 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -168,18 +168,6 @@ workflow SMRNASEQ {
         }
     }
 
-    //
-    // SUBWORKFLOW: Deduplicate UMIs by mapping them to the genome
-    //
-    // if (params.with_umi){
-    //     DEDUPLICATE_UMIS (
-    //         ch_bowtie_index,
-    //         ch_reads_for_mirna,
-    //     )
-    //     ch_reads_for_mirna = DEDUPLICATE_UMIS.out.reads
-    //     ch_versions = ch_versions.mix(DEDUPLICATE_UMIS.out.versions)
-    // }
-
     //UMI Dedup for fastq input
     if (params.with_umi) {
         ch_fastq = Channel.value('fastq')
@@ -196,6 +184,7 @@ workflow SMRNASEQ {
     .join( ch_reads_for_mirna )
     .map { meta, adapter_seq, reads -> [adapter_seq, meta.id, reads] }
     .groupTuple()
+    .dump()
     .set { ch_mirtrace_inputs }
 
     MIRTRACE(ch_mirtrace_inputs)

From 0d6ffadcc1b0f391ef826082d544d2cafcd86f8a Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 10:00:50 +0000
Subject: [PATCH 142/227] Cleaned up modules.config

---
 conf/modules.config | 60 +--------------------------------------------
 1 file changed, 1 insertion(+), 59 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 3cebdc02..2e571ec2 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -126,45 +126,7 @@ process {
     //
     // UMI deduplication
     //
-    withName: '.*:DEDUPLICATE_UMIS:UMI_MAP_GENOME' {
-        publishDir = [
-            path: { "${params.outdir}/umi_dedup/bam_mapped" },
-            mode: params.publish_dir_mode,
-            pattern: '*.bam',
-            enabled: (
-                params.save_umi_intermeds
-            )
-        ]
-    }
-    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' {
-        ext.prefix = { "${meta.id}.sorted" }
-        publishDir = [
-            path: { "${params.outdir}/umi_dedup/bam_mapped_sorted" },
-            mode: params.publish_dir_mode,
-            pattern: '*.{bam}',
-            enabled: (
-                params.save_umi_intermeds
-            )
-        ]
-    }
-    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX' {
-        ext.prefix = { "${meta.id}.sorted" }
-        publishDir = [
-            path: { "${params.outdir}/umi_dedup/bam_mapped_sorted" },
-            mode: params.publish_dir_mode,
-            pattern: '*.{bai,csi}',
-            enabled: (
-                params.save_umi_intermeds
-            )
-        ]
-    }
-    withName: '.*:DEDUPLICATE_UMIS:BAM_SORT_STATS_SAMTOOLS:.*' {
-        publishDir = [
-            path: { "${params.outdir}/umi_dedup/bam_mapped_sorted" },
-            mode: params.publish_dir_mode,
-            pattern: '*.{stats,flagstat,idxstats}'
-        ]
-    }
+
     withName: '.*:UMICOLLAPSE_FASTQ' {
         ext.args = { meta.single_end ? "--algo ${params.umitools_method} --two-pass" : "--method ${params.umitools_method} --two-pass --paired --remove-unpaired --remove-chimeric" }
         ext.prefix = { "${meta.id}.umi_dedup.sorted" }
@@ -177,26 +139,6 @@ process {
             )
         ]
     }
-    withName: '.*:DEDUPLICATE_UMIS:SAMTOOLS_BAM2FQ' {
-        publishDir = [
-            path: { "${params.outdir}/umi_dedup/fastq_deduplicated" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
-            enabled: (
-                params.save_umi_intermeds
-            )
-        ]
-    }
-    withName: '.*:DEDUPLICATE_UMIS:FASTQC_DEDUPLICATED' {
-        //the prefix is required for multiqc to pickup the files separately from the other fastqc instances
-        ext.prefix =  { "${meta.id}.deduplicated" }
-        ext.args = '--quiet'
-        publishDir = [
-            path: { "${params.outdir}/fastqc/deduplicated" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
 
     //
     // MIRTRACE QC

From da619d52fe074200e7b138a74626fb8c995f2588 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 10:46:00 +0000
Subject: [PATCH 143/227] Small modifications to make mirtrace run again

---
 modules/nf-core/umicollapse/main.nf | 2 +-
 workflows/smrnaseq.nf               | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 321ffd84..01915edb 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -14,7 +14,7 @@ process UMICOLLAPSE {
 
     output:
     tuple val(meta), path("*.bam"), emit: bam,                         optional: true
-    tuple val(meta), path("${prefix}.${extension}"), emit: fastq,      optional: true
+    tuple val(meta), path("*dedup*fastq.gz"), emit: fastq,      optional: true
     tuple val(meta), path("*_UMICollapse.log"), emit: log
     path  "versions.yml"          , emit: versions
 
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index f84b8f86..39776476 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -182,9 +182,9 @@ workflow SMRNASEQ {
     //
     FASTQ_FASTQC_UMITOOLS_FASTP.out.adapter_seq
     .join( ch_reads_for_mirna )
+    .dump()
     .map { meta, adapter_seq, reads -> [adapter_seq, meta.id, reads] }
     .groupTuple()
-    .dump()
     .set { ch_mirtrace_inputs }
 
     MIRTRACE(ch_mirtrace_inputs)

From c5b9685966949b6fd0744ac915d3b6b4d8bdbc31 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 10:50:08 +0000
Subject: [PATCH 144/227] Add versions

---
 workflows/smrnaseq.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 39776476..1cd81037 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -174,7 +174,8 @@ workflow SMRNASEQ {
         ch_input_for_collapse = ch_reads_for_mirna.map{ meta, reads -> [meta, reads, []]} //Needs to be done to add a []
         UMICOLLAPSE_FASTQ(ch_input_for_collapse, ch_fastq)
         ch_reads_for_mirna = UMICOLLAPSE_FASTQ.out.fastq
-    }
+        ch_versions = ch_versions.mix(UMICOLLAPSE_FASTQ.out.versions)
+        }
 
 
     //

From 9fc7d0a3e0226df3190f4fa5842ff16f0d9672a8 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 10:53:47 +0000
Subject: [PATCH 145/227] Yes, i wrote some docs

---
 docs/usage.md | 12 ++++++++++++
 1 file changed, 12 insertions(+)

diff --git a/docs/usage.md b/docs/usage.md
index f659727b..94f3a61c 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -54,6 +54,18 @@ Contamination filtering of the sequencing reads is optional and can be invoked u
 - `pirna`: Used to supply a FASTA file containing piRNA contamination sequence. e.g. The FASTA file is first compared to the available miRNA sequences and overlaps are removed.
 - `other_contamination`: Used to supply an additional filtering set. The FASTA file is first compared to the available miRNA sequences and overlaps are removed.
 
+### UMI handling
+
+The pipeline handles UMIs with two tools `Umitools-extract` and subsequently `Umicollapse` to deduplicate using UMI information. This can be achieved by using the parameters for UMI handling:
+
+```bash
+--with_umi --umitools_bc_pattern = '.+AACTGTAGGCACCATCAAT{s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
+```
+
+:::note
+You will have to specify custom `umitools_bc_pattern` patterns if your UMI is different. Please check the required capability in your UMI handling manual.
+:::
+
 ## Samplesheet input
 
 You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 2 columns ("sample" and "fastq_1"), and a header row as shown in the examples below.

From 7bf9266a43dd9bf42dea94ecbbb3d08c69c80f6c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 10:56:24 +0000
Subject: [PATCH 146/227] Some more docs

---
 docs/output.md | 13 ++++++-------
 1 file changed, 6 insertions(+), 7 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 537f17c5..d2358724 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -14,7 +14,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 - [FastQC](#fastqc) - read quality control
 - [UMI-tools extract](#umi-tools-extract) - UMI barcode extraction
-- [UMI-tools deduplicate](#umi-tools-deduplicate) - read deduplication
+- [UMI-collapse deduplicate](#umicollapse-deduplicate) - read deduplication
 - [FastP](#fastp) - adapter trimming
 - [Bowtie2](#bowtie2) - contamination filtering
 - [Bowtie](#bowtie) - alignment against mature miRNAs and miRNA precursors (hairpins)
@@ -53,7 +53,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 </details>
 
-[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name. Secondly, reads are deduplicated based on UMI identifier after mapping as highlighted in the [UMI-tools deduplicate](#umi-tools-deduplicate) section.
+[UMI-tools](https://github.com/CGATOxford/UMI-tools) extracts UMIs from reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name. Secondly, reads are deduplicated based on UMI identifier after mapping as highlighted in the [UMI-collapse deduplicate](#umicollapse-deduplicate) section.
 
 To facilitate processing of input data which has the UMI barcode already embedded in the read name from the start, `--skip_umi_extract` can be specified in conjunction with `--with_umi`.
 
@@ -72,18 +72,17 @@ Contains FastQ files with quality and adapter trimmed reads for each sample, alo
 
 FastP can automatically detect adapter sequences when not specified directly by the user - the pipeline also comes with a feature and a supplied miRNA adapters file to ensure adapters auto-detected are more accurate. If there are needs to add more known miRNA adapters to this list, please open a pull request.
 
-## UMI-tools deduplicate
+## UMI-collapse deduplicate
 
 <details markdown="1">
 <summary>Output files</summary>
 
 - `umi_dedup/`
-  - `*.tsv`: Results statistics files detailing the UMI deduplication results.
-  - `*.bam`: If `--save_umi_intermeds` is specified, the deduplicated bam files **after** UMI deduplication will be placed in this directory. In addition the sorted and indexed files will be placed there as well.
-  - `samtools_stats/` - `*.{stats,flagstat,idxstats}:` Statistics on the mappings underlying the UMI deduplication.
+  - `*.log`: Results statistics files detailing the UMI deduplication results.
+  - `*.fastq.gz`: If `--save_umi_intermeds` is specified, the deduplicated fastq.gz files **after** UMI deduplication will be placed in this directory.
   </details>
 
-[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name as highlighted in the [UMI-tools extract](#umi-tools-extract) section. The reads are deduplicated based on an alignment against the full genome of the species. The deduplicated reads are then converted into fastq format. The resulting fastq files are used in the remaining steps of the pipeline.
+[UMI-tools](https://github.com/CGATOxford/UMI-tools) deduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-tools `extract` command removes the UMI barcode information from the read sequence and adds it to the read name as highlighted in the [UMI-tools extract](#umi-tools-extract) section. Umicollapse works directly on the fastq files instead of mapping the UMI data first, then deduplicating and generating fastq files again.
 
 ## Bowtie2
 

From f5f17fd42da5f922ffb4c2b5ca504981e3bf9aec Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 11:12:56 +0000
Subject: [PATCH 147/227] Update umicollapse

---
 modules.json                                        | 2 +-
 modules/nf-core/umicollapse/main.nf                 | 8 ++++----
 modules/nf-core/umicollapse/tests/main.nf.test.snap | 8 ++++----
 3 files changed, 9 insertions(+), 9 deletions(-)

diff --git a/modules.json b/modules.json
index 8cb6236a..b846456d 100644
--- a/modules.json
+++ b/modules.json
@@ -62,7 +62,7 @@
                     },
                     "umicollapse": {
                         "branch": "master",
-                        "git_sha": "0f0531e44e5d106fc7ef4aadfb23352b09da80a9",
+                        "git_sha": "6971511e34fb6563a48f1bf583238a7c49654910",
                         "installed_by": ["modules"]
                     },
                     "umitools/extract": {
diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 01915edb..0641fddc 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -13,10 +13,10 @@ process UMICOLLAPSE {
     val(mode)
 
     output:
-    tuple val(meta), path("*.bam"), emit: bam,                         optional: true
-    tuple val(meta), path("*dedup*fastq.gz"), emit: fastq,      optional: true
-    tuple val(meta), path("*_UMICollapse.log"), emit: log
-    path  "versions.yml"          , emit: versions
+    tuple val(meta), path("*.bam"),                   emit: bam,        optional: true
+    tuple val(meta), path("*dedup*fastq.gz"),         emit: fastq,      optional: true
+    tuple val(meta), path("*_UMICollapse.log"),       emit: log
+    path  "versions.yml" ,                            emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/nf-core/umicollapse/tests/main.nf.test.snap b/modules/nf-core/umicollapse/tests/main.nf.test.snap
index 5cee4d4c..900ff08f 100644
--- a/modules/nf-core/umicollapse/tests/main.nf.test.snap
+++ b/modules/nf-core/umicollapse/tests/main.nf.test.snap
@@ -14,7 +14,7 @@
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
-        "timestamp": "2024-01-30T07:40:40.750602889"
+        "timestamp": "2024-01-30T10:45:28.352669757"
     },
     "umicollapse fastq tests": {
         "content": [
@@ -31,7 +31,7 @@
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
-        "timestamp": "2024-01-30T07:41:28.990304042"
+        "timestamp": "2024-01-30T10:45:56.053352008"
     },
     "umicollapse stub tests": {
         "content": [
@@ -86,7 +86,7 @@
                 ]
             }
         ],
-        "timestamp": "2024-01-30T07:41:55.110612801"
+        "timestamp": "2024-01-30T10:46:12.482697713"
     },
     "umicollapse paired tests": {
         "content": [
@@ -103,6 +103,6 @@
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
-        "timestamp": "2024-01-30T07:41:07.399188554"
+        "timestamp": "2024-01-30T10:45:44.961261065"
     }
 }
\ No newline at end of file

From 12980f83ad7fa8afc3e6abadd3cb1a4bb2acf48f Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 11:25:23 +0000
Subject: [PATCH 148/227] Less warnings

---
 subworkflows/local/genome_quant.nf | 1 -
 subworkflows/local/input_check.nf  | 4 +++-
 subworkflows/local/mirna_quant.nf  | 2 ++
 subworkflows/local/mirtrace.nf     | 4 +++-
 4 files changed, 8 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/genome_quant.nf b/subworkflows/local/genome_quant.nf
index 0f97f16b..c56f8e5f 100644
--- a/subworkflows/local/genome_quant.nf
+++ b/subworkflows/local/genome_quant.nf
@@ -2,7 +2,6 @@
 // Quantify mirna with bowtie and mirtop
 //
 
-include { INDEX_GENOME      } from '../../modules/local/bowtie_genome'
 include { BAM_SORT_STATS_SAMTOOLS } from '../nf-core/bam_sort_stats_samtools'
 include { BOWTIE_MAP_SEQ as BOWTIE_MAP_GENOME } from '../../modules/local/bowtie_map_mirna'
 
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index 910afd05..c7720312 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -9,15 +9,17 @@ workflow INPUT_CHECK {
     samplesheet // file: /path/to/samplesheet.csv
 
     main:
+    ch_versions = Channel.empty()
     SAMPLESHEET_CHECK ( samplesheet )
         .csv
         .splitCsv ( header:true, sep:',' )
         .map { create_fastq_channel(it) }
         .set { reads }
+    ch_versions.mix(SAMPLESHEET_CHECK.out.versions)
 
     emit:
     reads                                     // channel: [ val(meta), [ reads ] ]
-    versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ]
+    versions =  ch_versions                   // channel: [ versions.yml ]
 }
 
 // Function to get list of [ meta, [ fastq_1 ] ]
diff --git a/subworkflows/local/mirna_quant.nf b/subworkflows/local/mirna_quant.nf
index fc6942fe..f11cb234 100644
--- a/subworkflows/local/mirna_quant.nf
+++ b/subworkflows/local/mirna_quant.nf
@@ -84,6 +84,8 @@ workflow MIRNA_QUANT {
         .set { edger_input }
     EDGER_QC ( edger_input )
 
+    ch_versions.mix(EDGER_QC.out.versions)
+
     reads
         .map { add_suffix(it, "seqcluster") }
         .dump (tag:'ssux')
diff --git a/subworkflows/local/mirtrace.nf b/subworkflows/local/mirtrace.nf
index 317c4444..c5a5f492 100644
--- a/subworkflows/local/mirtrace.nf
+++ b/subworkflows/local/mirtrace.nf
@@ -9,9 +9,11 @@ workflow MIRTRACE {
     reads      // channel: [ val(adapterseq), [ val(ids) ], [ path(reads) ] ]
 
     main:
+    ch_versions = Channel.empty()
     reads | MIRTRACE_RUN
+    ch_versions.mix(MIRTRACE_RUN.out.versions)
 
     emit:
     results    = MIRTRACE_RUN.out.mirtrace
-    versions   = MIRTRACE_RUN.out.versions
+    versions   = ch_versions
 }

From 9cdb1c8190da94a9f9839d94d09c4dd986eec74c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 11:36:54 +0000
Subject: [PATCH 149/227] aDJUST

---
 CHANGELOG.md                   |  3 ++-
 lib/WorkflowSmrnaseq.groovy    |  2 --
 nextflow_schema.json           |  7 ++++---
 subworkflows/local/mirtrace.nf | 19 -------------------
 workflows/smrnaseq.nf          |  6 +++---
 5 files changed, 9 insertions(+), 28 deletions(-)
 delete mode 100644 subworkflows/local/mirtrace.nf

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 18232b5f..8a4389cc 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.3.0 - 2024-01-25 - Gray Zinc Dalmatian
+## v2.3.0 - 2024-01-31 - Gray Zinc Dalmatian
 
 - [[#307]](https://github.com/nf-core/smrnaseq/pull/307) - Clean up config file and improve output folder structure
 - [[#299]](https://github.com/nf-core/smrnaseq/issues/299) - Bugfix for missing inputs in BAM stats (`genome_quant.r`)
@@ -11,6 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [[#302]](https://github.com/nf-core/smrnaseq/pull/302) - Merged in nf-core template v2.11.1
 - [[#294]](https://github.com/nf-core/smrnaseq/pull/294) - Fixed contamination screening issues
 - [[#309]](https://github.com/nf-core/smrnaseq/pull/309) - Merged in nf-core template v2.12.0
+- [[#310]](https://github.com/nf-core/smrnaseq/pull/310) - Removed unnecessarily separate mirtrace subworkflow, now using module instead
 
 ### Parameters
 
diff --git a/lib/WorkflowSmrnaseq.groovy b/lib/WorkflowSmrnaseq.groovy
index 2575fd9b..b07c388a 100755
--- a/lib/WorkflowSmrnaseq.groovy
+++ b/lib/WorkflowSmrnaseq.groovy
@@ -48,8 +48,6 @@ class WorkflowSmrnaseq {
 
     public static String toolCitationText(params) {
 
-        // TODO nf-core: Optionally add in-text citation tools to this list.
-
         // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "",
         // Uncomment function in methodsDescriptionText to render in MultiQC report
         def citation_text = [
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 368e85ac..6e1b50a9 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -251,6 +251,10 @@
                     "default": 10,
                     "fa_icon": "far fa-window-minimize",
                     "description": "Minimum number of reads required in input file to use it"
+                },
+                "save_merged": {
+                    "type": "boolean",
+                    "description": "Save merged reads."
                 }
             }
         },
@@ -515,9 +519,6 @@
                     "description": "Validation of parameters in lenient more.",
                     "hidden": true,
                     "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)."
-                },
-                "save_merged": {
-                    "type": "boolean"
                 }
             }
         }
diff --git a/subworkflows/local/mirtrace.nf b/subworkflows/local/mirtrace.nf
deleted file mode 100644
index c5a5f492..00000000
--- a/subworkflows/local/mirtrace.nf
+++ /dev/null
@@ -1,19 +0,0 @@
-//
-// Quantify mirna with bowtie and mirtop
-//
-
-include { MIRTRACE_RUN } from '../../modules/local/mirtrace'
-
-workflow MIRTRACE {
-    take:
-    reads      // channel: [ val(adapterseq), [ val(ids) ], [ path(reads) ] ]
-
-    main:
-    ch_versions = Channel.empty()
-    reads | MIRTRACE_RUN
-    ch_versions.mix(MIRTRACE_RUN.out.versions)
-
-    emit:
-    results    = MIRTRACE_RUN.out.mirtrace
-    versions   = ch_versions
-}
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 1cd81037..c83acd69 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -69,9 +69,9 @@ include { FASTQ_FASTQC_UMITOOLS_FASTP } from '../subworkflows/nf-core/fastq_fast
 include { CONTAMINANT_FILTER          } from '../subworkflows/local/contaminant_filter'
 include { MIRNA_QUANT                 } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT                } from '../subworkflows/local/genome_quant'
-include { MIRTRACE                    } from '../subworkflows/local/mirtrace'
 include { MIRDEEP2                    } from '../subworkflows/local/mirdeep2'
 include { INDEX_GENOME                } from '../modules/local/bowtie_genome'
+include { MIRTRACE_RUN as MIRTRACE         } from '../modules/local/mirtrace'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -179,7 +179,7 @@ workflow SMRNASEQ {
 
 
     //
-    // SUBWORKFLOW: mirtrace QC
+    // MODULE: mirtrace QC
     //
     FASTQ_FASTQC_UMITOOLS_FASTP.out.adapter_seq
     .join( ch_reads_for_mirna )
@@ -276,7 +276,7 @@ workflow SMRNASEQ {
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mature_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.hairpin_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mirtop_logs.collect().ifEmpty([]))
-        ch_multiqc_files = ch_multiqc_files.mix(MIRTRACE.out.results.collect().ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(MIRTRACE.out.mirtrace.collect().ifEmpty([]))
 
         MULTIQC (
             ch_multiqc_files.collect(),

From 7515e60bed42ca2ffcdd6c84bf6e2a06029b86bc Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Tue, 30 Jan 2024 16:38:38 +0000
Subject: [PATCH 150/227] Fix the stacksize

---
 modules/nf-core/umicollapse/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index 0641fddc..ace35e5c 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -42,7 +42,7 @@ process UMICOLLAPSE {
     UMICOLLAPSE_JAR=\$(dirname \$(which umicollapse))/../share/umicollapse-${VERSION}/umicollapse.jar
     java \\
         -Xmx${max_heap_size_mega}M \\
-        -Xss${max_stack_size_mega}M \\
+        -Xss999M \\
         -jar \$UMICOLLAPSE_JAR \\
         $mode \\
         -i ${input} \\

From 55bfd6d808abe35af471afacb149b1a3c593f27d Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 31 Jan 2024 09:59:35 +0000
Subject: [PATCH 151/227] Use the pipe Luke

---
 workflows/smrnaseq.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index c83acd69..8c92ae30 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -71,7 +71,7 @@ include { MIRNA_QUANT                 } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT                } from '../subworkflows/local/genome_quant'
 include { MIRDEEP2                    } from '../subworkflows/local/mirdeep2'
 include { INDEX_GENOME                } from '../modules/local/bowtie_genome'
-include { MIRTRACE_RUN as MIRTRACE         } from '../modules/local/mirtrace'
+include { MIRTRACE_RUN as MIRTRACE    } from '../modules/local/mirtrace'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -188,7 +188,7 @@ workflow SMRNASEQ {
     .groupTuple()
     .set { ch_mirtrace_inputs }
 
-    MIRTRACE(ch_mirtrace_inputs)
+    ch_mirtrace_inputs | MIRTRACE
     ch_versions = ch_versions.mix(MIRTRACE.out.versions.ifEmpty(null))
 
 

From ba2c5a80e1657c277b3deddba3f147b9bd442c17 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 31 Jan 2024 10:10:31 +0000
Subject: [PATCH 152/227] mirtrace back

---
 subworkflows/local/mirtrace.nf | 17 +++++++++++++++++
 workflows/smrnaseq.nf          |  4 ++--
 2 files changed, 19 insertions(+), 2 deletions(-)
 create mode 100644 subworkflows/local/mirtrace.nf

diff --git a/subworkflows/local/mirtrace.nf b/subworkflows/local/mirtrace.nf
new file mode 100644
index 00000000..317c4444
--- /dev/null
+++ b/subworkflows/local/mirtrace.nf
@@ -0,0 +1,17 @@
+//
+// Quantify mirna with bowtie and mirtop
+//
+
+include { MIRTRACE_RUN } from '../../modules/local/mirtrace'
+
+workflow MIRTRACE {
+    take:
+    reads      // channel: [ val(adapterseq), [ val(ids) ], [ path(reads) ] ]
+
+    main:
+    reads | MIRTRACE_RUN
+
+    emit:
+    results    = MIRTRACE_RUN.out.mirtrace
+    versions   = MIRTRACE_RUN.out.versions
+}
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 8c92ae30..03373e79 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -71,7 +71,6 @@ include { MIRNA_QUANT                 } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT                } from '../subworkflows/local/genome_quant'
 include { MIRDEEP2                    } from '../subworkflows/local/mirdeep2'
 include { INDEX_GENOME                } from '../modules/local/bowtie_genome'
-include { MIRTRACE_RUN as MIRTRACE    } from '../modules/local/mirtrace'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -86,6 +85,7 @@ include { CAT_FASTQ                        } from '../modules/nf-core/cat/fastq/
 include { MULTIQC                          } from '../modules/nf-core/multiqc/main'
 include { CUSTOM_DUMPSOFTWAREVERSIONS      } from '../modules/nf-core/custom/dumpsoftwareversions/main'
 include { UMICOLLAPSE as UMICOLLAPSE_FASTQ } from '../modules/nf-core/umicollapse/main'
+include { MIRTRACE                         } from '../subworkflows/local/mirtrace'
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -188,7 +188,7 @@ workflow SMRNASEQ {
     .groupTuple()
     .set { ch_mirtrace_inputs }
 
-    ch_mirtrace_inputs | MIRTRACE
+    MIRTRACE(ch_mirtrace_inputs)
     ch_versions = ch_versions.mix(MIRTRACE.out.versions.ifEmpty(null))
 
 

From b8fb3cb850891e60fe90199a7184aa8d05eacab9 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 31 Jan 2024 10:12:01 +0000
Subject: [PATCH 153/227] Bugfix

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 03373e79..fc364194 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -276,7 +276,7 @@ workflow SMRNASEQ {
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mature_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.hairpin_stats.collect({it[1]}).ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(MIRNA_QUANT.out.mirtop_logs.collect().ifEmpty([]))
-        ch_multiqc_files = ch_multiqc_files.mix(MIRTRACE.out.mirtrace.collect().ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(MIRTRACE.out.results.collect().ifEmpty([]))
 
         MULTIQC (
             ch_multiqc_files.collect(),

From 422b8ffc6913e9d7c3328896402e9554ecccf265 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 31 Jan 2024 12:51:21 +0000
Subject: [PATCH 154/227] fastq mode, we need to not extract the UMIs

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 86558361..0f923d42 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -31,7 +31,7 @@ params {
 
     // UMI handling
     with_umi                   = false
-    skip_umi_extract           = false
+    skip_umi_extract           = true //Needs to be true for fastq mode in collapsing reads
     umitools_extract_method    = 'string'
     umitools_bc_pattern        = null
     umi_discard_read           = null

From 38a4255618ace37e60beb2952f2773d58e10561b Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 31 Jan 2024 15:56:54 +0000
Subject: [PATCH 155/227] Adjusted to run umiextract after umicollapse

---
 workflows/smrnaseq.nf | 12 +++++++++---
 1 file changed, 9 insertions(+), 3 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index fc364194..c4ea56ea 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -71,6 +71,7 @@ include { MIRNA_QUANT                 } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT                } from '../subworkflows/local/genome_quant'
 include { MIRDEEP2                    } from '../subworkflows/local/mirdeep2'
 include { INDEX_GENOME                } from '../modules/local/bowtie_genome'
+include { MIRTRACE                    } from '../subworkflows/local/mirtrace'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -85,7 +86,8 @@ include { CAT_FASTQ                        } from '../modules/nf-core/cat/fastq/
 include { MULTIQC                          } from '../modules/nf-core/multiqc/main'
 include { CUSTOM_DUMPSOFTWAREVERSIONS      } from '../modules/nf-core/custom/dumpsoftwareversions/main'
 include { UMICOLLAPSE as UMICOLLAPSE_FASTQ } from '../modules/nf-core/umicollapse/main'
-include { MIRTRACE                         } from '../subworkflows/local/mirtrace'
+include { UMITOOLS_EXTRACT                 } from '../../../modules/nf-core/umitools/extract/main'
+
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -169,13 +171,17 @@ workflow SMRNASEQ {
     }
 
     //UMI Dedup for fastq input
+    // This involves running on the sequencing adapter trimmed remnants of the entire reads
+    // consisting of sequence + common sequence "miRNA adapter" + UMI
+    // once collapsing happened, we will use umitools extract to get rid of the common miRNA sequence + the UMI to have only plain collapsed reads without any other clutter
     if (params.with_umi) {
         ch_fastq = Channel.value('fastq')
         ch_input_for_collapse = ch_reads_for_mirna.map{ meta, reads -> [meta, reads, []]} //Needs to be done to add a []
         UMICOLLAPSE_FASTQ(ch_input_for_collapse, ch_fastq)
-        ch_reads_for_mirna = UMICOLLAPSE_FASTQ.out.fastq
         ch_versions = ch_versions.mix(UMICOLLAPSE_FASTQ.out.versions)
-        }
+        UMITOOLS_EXTRACT(UMICOLLAPSE_FASTQ.out.fastq)
+        ch_reads_for_mirna = UMITOOLS_EXTRACT.out.reads
+    }
 
 
     //

From 3b646d6ff855008a8631560e835d8aa882ca1df5 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 31 Jan 2024 15:59:54 +0000
Subject: [PATCH 156/227] Removed miRNA sequencing adapters due to new mode of
 data handling

---
 assets/known_adapters.fa | 6 +-----
 1 file changed, 1 insertion(+), 5 deletions(-)

diff --git a/assets/known_adapters.fa b/assets/known_adapters.fa
index 6b54be80..729d233a 100644
--- a/assets/known_adapters.fa
+++ b/assets/known_adapters.fa
@@ -1,6 +1,2 @@
-> QIAseq miRNA adapter
-AACTGTAGGCACCATCAAT
-> Illumina miRNA adapter
-TGGAATTCTCGGGTGCCAAGG
-> Nextflex miRNA adapter
+> Illumina Sequencing Adapter
 TGGAATTCTCGGGTGCCAAGG

From e64ea1c478c971bdc111615e3660dae6079ad0bd Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 1 Feb 2024 07:11:34 +0000
Subject: [PATCH 157/227] Fix wrong path

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index c4ea56ea..5dfaafd8 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -86,7 +86,7 @@ include { CAT_FASTQ                        } from '../modules/nf-core/cat/fastq/
 include { MULTIQC                          } from '../modules/nf-core/multiqc/main'
 include { CUSTOM_DUMPSOFTWAREVERSIONS      } from '../modules/nf-core/custom/dumpsoftwareversions/main'
 include { UMICOLLAPSE as UMICOLLAPSE_FASTQ } from '../modules/nf-core/umicollapse/main'
-include { UMITOOLS_EXTRACT                 } from '../../../modules/nf-core/umitools/extract/main'
+include { UMITOOLS_EXTRACT                 } from '../modules/nf-core/umitools/extract/main'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From 9fd91090d5b73946d9a165147d78cddbd2e5fde6 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 1 Feb 2024 07:13:39 +0000
Subject: [PATCH 158/227] Configuration

---
 conf/modules.config | 39 ++++++++++++++++++++-------------------
 1 file changed, 20 insertions(+), 19 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 2e571ec2..1fc42838 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -92,25 +92,6 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:UMITOOLS_EXTRACT' {
-        ext.args  = [
-            params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
-            params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
-        ].join(' ').trim()
-        publishDir = [
-            [
-                path: { "${params.outdir}/umi_dedup/fastq_extracted_umi" },
-                mode: params.publish_dir_mode,
-                pattern: "*.log"
-            ],
-            [
-                path: { "${params.outdir}/umi_dedup/fastq_extracted_umi" },
-                mode: params.publish_dir_mode,
-                pattern: "*.fastq.gz",
-                enabled: params.save_umi_intermeds
-            ]
-        ]
-    }
 
     //
     // Make bowtie index
@@ -140,6 +121,26 @@ process {
         ]
     }
 
+    withName: 'UMITOOLS_EXTRACT' {
+        ext.args  = [
+            params.umitools_extract_method ? "--extract-method=${params.umitools_extract_method}" : '',
+            params.umitools_bc_pattern     ? "--bc-pattern='${params.umitools_bc_pattern}'" : '',
+        ].join(' ').trim()
+        publishDir = [
+            [
+                path: { "${params.outdir}/umi_dedup/fastq_extracted_umi" },
+                mode: params.publish_dir_mode,
+                pattern: "*.log"
+            ],
+            [
+                path: { "${params.outdir}/umi_dedup/fastq_extracted_umi" },
+                mode: params.publish_dir_mode,
+                pattern: "*.fastq.gz",
+                enabled: params.save_umi_intermeds
+            ]
+        ]
+    }
+
     //
     // MIRTRACE QC
     //

From e83b91077ec6ea92014026adb220b794ba78c712 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 1 Feb 2024 07:17:25 +0000
Subject: [PATCH 159/227] ANd modules update

---
 modules.json                                  |  4 +-
 modules/nf-core/fastqc/tests/main.nf.test     | 14 ++--
 .../nf-core/fastqc/tests/main.nf.test.snap    | 76 ++++++++++++++++++-
 modules/nf-core/umicollapse/main.nf           |  4 +-
 4 files changed, 83 insertions(+), 15 deletions(-)

diff --git a/modules.json b/modules.json
index b846456d..168be565 100644
--- a/modules.json
+++ b/modules.json
@@ -27,7 +27,7 @@
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9",
+                        "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
                         "installed_by": ["fastq_fastqc_umitools_fastp"]
                     },
                     "multiqc": {
@@ -62,7 +62,7 @@
                     },
                     "umicollapse": {
                         "branch": "master",
-                        "git_sha": "6971511e34fb6563a48f1bf583238a7c49654910",
+                        "git_sha": "2e561f4551ce3df2bc60016f7ea359f2ed4bce49",
                         "installed_by": ["modules"]
                     },
                     "umitools/extract": {
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
index 1f21c664..70edae4d 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -33,7 +33,7 @@ nextflow_process {
             { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
             { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_single") }
             )
         }
     }
@@ -63,7 +63,7 @@ nextflow_process {
             { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
             { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_paired") }
             )
         }
     }
@@ -89,7 +89,7 @@ nextflow_process {
             { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
             { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") }
             )
         }
     }
@@ -115,7 +115,7 @@ nextflow_process {
             { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
             { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_bam") }
             )
         }
     }
@@ -153,7 +153,7 @@ nextflow_process {
             { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
             { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_multiple") }
             )
         }
     }
@@ -179,7 +179,7 @@ nextflow_process {
             { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
             { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") }
             )
         }
     }
@@ -204,7 +204,7 @@ nextflow_process {
             { assert process.success },
             { assert snapshot(process.out.html.collect { file(it[1]).getName() } +
                                 process.out.zip.collect { file(it[1]).getName() } +
-                                process.out.versions ).match() }
+                                process.out.versions ).match("fastqc_stub") }
             )
         }
     }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
index 5d624bb8..86f7c311 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test.snap
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -1,5 +1,17 @@
 {
-    "sarscov2 single-end [fastq] - stub": {
+    "fastqc_versions_interleaved": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:40:07.293713"
+    },
+    "fastqc_stub": {
         "content": [
             [
                 "test.html",
@@ -7,14 +19,70 @@
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
-        "timestamp": "2024-01-17T18:40:57.254299"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:31:01.425198"
+    },
+    "fastqc_versions_multiple": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:40:55.797907"
+    },
+    "fastqc_versions_bam": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:40:26.795862"
+    },
+    "fastqc_versions_single": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:39:27.043675"
+    },
+    "fastqc_versions_paired": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:39:47.584191"
     },
-    "versions": {
+    "fastqc_versions_custom_prefix": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
-        "timestamp": "2024-01-17T18:36:50.033627"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:41:14.576531"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/umicollapse/main.nf b/modules/nf-core/umicollapse/main.nf
index ace35e5c..dae290e6 100644
--- a/modules/nf-core/umicollapse/main.nf
+++ b/modules/nf-core/umicollapse/main.nf
@@ -29,7 +29,7 @@ process UMICOLLAPSE {
     // umicollapse. We set the stack size to 5% of the available memory, the heap size to 90%
     // which leaves 5% for stuff happening outside of java without the scheduler killing the process.
     def max_heap_size_mega = (task.memory.toMega() * 0.9).intValue()
-    def max_stack_size_mega = (task.memory.toMega() * 0.05).intValue()
+    def max_stack_size_mega = 999 //most java jdks will not allow Xss > 1GB, so fixing this to the allowed max
     if ( mode !in [ 'fastq', 'bam' ] ) {
         error "Mode must be one of 'fastq' or 'bam'."
     }
@@ -42,7 +42,7 @@ process UMICOLLAPSE {
     UMICOLLAPSE_JAR=\$(dirname \$(which umicollapse))/../share/umicollapse-${VERSION}/umicollapse.jar
     java \\
         -Xmx${max_heap_size_mega}M \\
-        -Xss999M \\
+        -Xss${max_stack_size_mega}M \\
         -jar \$UMICOLLAPSE_JAR \\
         $mode \\
         -i ${input} \\

From 6f5c91bc7a06d18d6c78f6d2c7b179813c1754e7 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 1 Feb 2024 08:12:00 +0000
Subject: [PATCH 160/227] UMI pattern was wrong

---
 conf/test_umi.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/test_umi.config b/conf/test_umi.config
index 48f61181..c7d0db15 100644
--- a/conf/test_umi.config
+++ b/conf/test_umi.config
@@ -31,6 +31,6 @@ params {
     //UMI Specific testcase
     with_umi = true
     umitools_extract_method = 'regex'
-    umitools_bc_pattern = '.+AACTGTAGGCACCATCAAT{s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
+    umitools_bc_pattern = '.+(?P<discard_1>AACTGTAGGCACCATCAAT){s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
     save_umi_intermeds = true
 }

From 2d7f5a903bdfca079f2f4cbf513fa99013d77e57 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Thu, 1 Feb 2024 16:11:40 +0000
Subject: [PATCH 161/227] add FASTP length filtering step after umi extract

---
 conf/modules.config               |  9 ++++++++
 subworkflows/local/mirna_quant.nf |  5 +----
 workflows/smrnaseq.nf             | 37 ++++++++++++++++++++-----------
 3 files changed, 34 insertions(+), 17 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 1fc42838..cd93bc98 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -41,6 +41,15 @@ process {
             // enabled: params.save_merged_fastq //TODO ? implement save_merged_fastq param
         ]
     }
+    withName: 'FASTP_LENGTH_FILTER' {
+        ext.args = [ "",
+            "--disable_adapter_trimming",
+            "--disable_quality_filtering",
+            params.clip_r1 > 0             ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
+            params.three_prime_clip_r1 > 0 ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.
+            params.fastp_min_length > 0    ? "-l ${params.fastp_min_length}" : ""
+        ].join(" ").trim()
+    }
 
     //
     // FASTQ_FASTQC_UMITOOLS_FASTP
diff --git a/subworkflows/local/mirna_quant.nf b/subworkflows/local/mirna_quant.nf
index f11cb234..aea1c9a7 100644
--- a/subworkflows/local/mirna_quant.nf
+++ b/subworkflows/local/mirna_quant.nf
@@ -58,8 +58,6 @@ workflow MIRNA_QUANT {
     BAM_STATS_MATURE ( BOWTIE_MAP_MATURE.out.bam, FORMAT_MATURE.out.formatted_fasta )
     ch_versions = ch_versions.mix(BAM_STATS_MATURE.out.versions)
 
-
-
     PARSE_HAIRPIN ( hairpin ).parsed_fasta.set { hairpin_parsed }
     ch_versions = ch_versions.mix(PARSE_HAIRPIN.out.versions)
 
@@ -75,15 +73,14 @@ workflow MIRNA_QUANT {
     BAM_STATS_HAIRPIN ( BOWTIE_MAP_HAIRPIN.out.bam, FORMAT_HAIRPIN.out.formatted_fasta )
     ch_versions = ch_versions.mix(BAM_STATS_HAIRPIN.out.versions)
 
-
     BAM_STATS_MATURE.out.idxstats.collect{it[1]}
         .mix(BAM_STATS_HAIRPIN.out.idxstats.collect{it[1]})
         .dump(tag:'edger')
         .flatten()
         .collect()
         .set { edger_input }
-    EDGER_QC ( edger_input )
 
+    EDGER_QC ( edger_input )
     ch_versions.mix(EDGER_QC.out.versions)
 
     reads
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 5dfaafd8..e2a76a9e 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -64,14 +64,15 @@ if (!params.mirgenedb) {
     if (params.mirgenedb_gff) { mirna_gtf = file(params.mirgenedb_gff, checkIfExists: true) } else { exit 1, "MirGeneDB gff file not found: ${params.mirgenedb_gff}"}
 }
 
-include { INPUT_CHECK                 } from '../subworkflows/local/input_check'
-include { FASTQ_FASTQC_UMITOOLS_FASTP } from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp'
-include { CONTAMINANT_FILTER          } from '../subworkflows/local/contaminant_filter'
-include { MIRNA_QUANT                 } from '../subworkflows/local/mirna_quant'
-include { GENOME_QUANT                } from '../subworkflows/local/genome_quant'
-include { MIRDEEP2                    } from '../subworkflows/local/mirdeep2'
-include { INDEX_GENOME                } from '../modules/local/bowtie_genome'
-include { MIRTRACE                    } from '../subworkflows/local/mirtrace'
+include { INPUT_CHECK                               } from '../subworkflows/local/input_check'
+include { FASTQ_FASTQC_UMITOOLS_FASTP               } from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp'
+include { FASTP as FASTP_LENGTH_FILTER              } from '../modules/nf-core/fastp'
+include { CONTAMINANT_FILTER                        } from '../subworkflows/local/contaminant_filter'
+include { MIRNA_QUANT                               } from '../subworkflows/local/mirna_quant'
+include { GENOME_QUANT                              } from '../subworkflows/local/genome_quant'
+include { MIRDEEP2                                  } from '../subworkflows/local/mirdeep2'
+include { INDEX_GENOME                              } from '../modules/local/bowtie_genome'
+include { MIRTRACE                                  } from '../subworkflows/local/mirtrace'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -136,7 +137,6 @@ workflow SMRNASEQ {
     //
     // SUBWORKFLOW: Read QC, extract UMI and trim adapters & dedup UMIs if necessary / desired by the user
     //
-
     FASTQ_FASTQC_UMITOOLS_FASTP (
         ch_cat_fastq,
         params.skip_fastqc,
@@ -170,7 +170,7 @@ workflow SMRNASEQ {
         }
     }
 
-    //UMI Dedup for fastq input
+    // UMI Dedup for fastq input
     // This involves running on the sequencing adapter trimmed remnants of the entire reads
     // consisting of sequence + common sequence "miRNA adapter" + UMI
     // once collapsing happened, we will use umitools extract to get rid of the common miRNA sequence + the UMI to have only plain collapsed reads without any other clutter
@@ -180,9 +180,18 @@ workflow SMRNASEQ {
         UMICOLLAPSE_FASTQ(ch_input_for_collapse, ch_fastq)
         ch_versions = ch_versions.mix(UMICOLLAPSE_FASTQ.out.versions)
         UMITOOLS_EXTRACT(UMICOLLAPSE_FASTQ.out.fastq)
-        ch_reads_for_mirna = UMITOOLS_EXTRACT.out.reads
-    }
 
+        // Filter out sequences smaller than params.fastp_min_length
+        FASTP_LENGTH_FILTER (
+            UMITOOLS_EXTRACT.out.reads,
+            params.fastp_known_mirna_adapters,
+            params.save_trimmed_fail,
+            params.save_merged
+        )
+        ch_versions = ch_versions.mix(FASTP_LENGTH_FILTER.out.versions)
+
+        ch_reads_for_mirna = FASTP_LENGTH_FILTER.out.reads
+    }
 
     //
     // MODULE: mirtrace QC
@@ -194,10 +203,12 @@ workflow SMRNASEQ {
     .groupTuple()
     .set { ch_mirtrace_inputs }
 
+    //
+    // SUBWORKFLOW: MIRTRACE
+    //
     MIRTRACE(ch_mirtrace_inputs)
     ch_versions = ch_versions.mix(MIRTRACE.out.versions.ifEmpty(null))
 
-
     //
     // SUBWORKFLOW: remove contaminants from reads
     //

From 8c1143d3a33496af0e27449db68ee2cfa8e8eaf8 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 2 Feb 2024 13:02:59 +0000
Subject: [PATCH 162/227] fix module config name of FASTP process, add publish
 dir for second FASTP process

---
 conf/modules.config | 48 +++++++++++++++++++++++++++++++++------------
 1 file changed, 35 insertions(+), 13 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index cd93bc98..3513870d 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -41,20 +41,11 @@ process {
             // enabled: params.save_merged_fastq //TODO ? implement save_merged_fastq param
         ]
     }
-    withName: 'FASTP_LENGTH_FILTER' {
-        ext.args = [ "",
-            "--disable_adapter_trimming",
-            "--disable_quality_filtering",
-            params.clip_r1 > 0             ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
-            params.three_prime_clip_r1 > 0 ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.
-            params.fastp_min_length > 0    ? "-l ${params.fastp_min_length}" : ""
-        ].join(" ").trim()
-    }
 
     //
     // FASTQ_FASTQC_UMITOOLS_FASTP
     //
-    withName: '.*:FASTQC_UMITOOLS_FASTP:FASTP' {
+    withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTP' {
         ext.args = [ "",
             params.trim_fastq              ? "" : "--disable_adapter_trimming",
             params.clip_r1 > 0             ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
@@ -65,17 +56,17 @@ process {
         ].join(" ").trim()
         publishDir = [
             [
-                path: { "${params.outdir}/fastp" },
+                path: { "${params.outdir}/fastp/umi_extracted" },
                 mode: params.publish_dir_mode,
                 pattern: "*.{json,html}"
             ],
             [
-                path: { "${params.outdir}/fastp/log" },
+                path: { "${params.outdir}/fastp/umi_extracted/log" },
                 mode: params.publish_dir_mode,
                 pattern: "*.log"
             ],
             [
-                path: { "${params.outdir}/fastp" },
+                path: { "${params.outdir}/fastp/umi_extracted" },
                 mode: params.publish_dir_mode,
                 pattern: "*.fail.fastq.gz",
                 enabled: params.save_trimmed_fail
@@ -102,6 +93,37 @@ process {
         ]
     }
 
+    //
+    // FASTP LENGTH FILTER
+    //
+    withName: 'FASTP_LENGTH_FILTER' {
+        ext.args = [ "",
+            "--disable_adapter_trimming",
+            "--disable_quality_filtering",
+            params.clip_r1 > 0             ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
+            params.three_prime_clip_r1 > 0 ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.
+            params.fastp_min_length > 0    ? "-l ${params.fastp_min_length}" : ""
+        ].join(" ").trim()
+        publishDir = [
+            [
+                path: { "${params.outdir}/fastp/length_filtered" },
+                mode: params.publish_dir_mode,
+                pattern: "*.{json,html}"
+            ],
+            [
+                path: { "${params.outdir}/fastp/length_filtered/log" },
+                mode: params.publish_dir_mode,
+                pattern: "*.log"
+            ],
+            [
+                path: { "${params.outdir}/fastp/length_filtered" },
+                mode: params.publish_dir_mode,
+                pattern: "*.fail.fastq.gz",
+                enabled: params.save_trimmed_fail
+            ]
+        ]
+    }
+
     //
     // Make bowtie index
     //

From f83f0a5e2e7a40476b8785c215e76be97d162ecf Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 2 Feb 2024 14:37:56 +0000
Subject: [PATCH 163/227] change default parameter values of FASTP max length
 and three prime adapter sequence, set adapter fasta based on with_umi
 parameter, change included adapter fasta

---
 assets/known_adapters.fa |  6 +++++-
 conf/modules.config      | 10 +++++-----
 nextflow.config          |  4 ++--
 workflows/smrnaseq.nf    |  6 ++++--
 4 files changed, 16 insertions(+), 10 deletions(-)

diff --git a/assets/known_adapters.fa b/assets/known_adapters.fa
index 729d233a..6b54be80 100644
--- a/assets/known_adapters.fa
+++ b/assets/known_adapters.fa
@@ -1,2 +1,6 @@
-> Illumina Sequencing Adapter
+> QIAseq miRNA adapter
+AACTGTAGGCACCATCAAT
+> Illumina miRNA adapter
+TGGAATTCTCGGGTGCCAAGG
+> Nextflex miRNA adapter
 TGGAATTCTCGGGTGCCAAGG
diff --git a/conf/modules.config b/conf/modules.config
index 3513870d..9c48f6f0 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -47,11 +47,11 @@ process {
     //
     withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTP' {
         ext.args = [ "",
-            params.trim_fastq              ? "" : "--disable_adapter_trimming",
-            params.clip_r1 > 0             ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
-            params.three_prime_clip_r1 > 0 ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.
-            params.fastp_min_length > 0    ? "-l ${params.fastp_min_length}" : "",
-            params.fastp_max_length > 0    ? "--max_len1 ${params.fastp_max_length}" : "",
+            params.trim_fastq                  ? "" : "--disable_adapter_trimming",
+            params.clip_r1 > 0                 ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1.
+            params.three_prime_clip_r1 > 0     ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.
+            params.fastp_min_length > 0        ? "-l ${params.fastp_min_length}" : "",
+            params.fastp_max_length > 0        ? "--max_len1 ${params.fastp_max_length}" : "",
             params.three_prime_adapter == null ?  '' : "--adapter_sequence ${params.three_prime_adapter}"
         ].join(" ").trim()
         publishDir = [
diff --git a/nextflow.config b/nextflow.config
index 0f923d42..34d26823 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -41,7 +41,7 @@ params {
     // Trimming options
     clip_r1                     = null
     three_prime_clip_r1         = null
-    three_prime_adapter         = null
+    three_prime_adapter         = 'AGATCGGAAGAGCACACGTCTGAACTCCAGTCA'
     trim_fastq                  = true
     fastp_min_length            = 17
     fastp_known_mirna_adapters  = "$projectDir/assets/known_adapters.fa"
@@ -52,7 +52,7 @@ params {
     skip_mirdeep                = false
     skip_fastp                  = false
     save_reference              = false
-    fastp_max_length            = 40
+    fastp_max_length            = 100
     min_trimmed_reads           = 10
 
     // Contamination filtering
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index e2a76a9e..f28f267a 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -134,6 +134,8 @@ workflow SMRNASEQ {
     .set { ch_cat_fastq }
     ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first().ifEmpty(null))
 
+    mirna_adapters = params.with_umi ? [] : params.fastp_known_mirna_adapters
+
     //
     // SUBWORKFLOW: Read QC, extract UMI and trim adapters & dedup UMIs if necessary / desired by the user
     //
@@ -144,7 +146,7 @@ workflow SMRNASEQ {
         params.skip_umi_extract,
         params.umi_discard_read,
         params.skip_fastp,
-        params.fastp_known_mirna_adapters,
+        mirna_adapters,
         params.save_trimmed_fail,
         params.save_merged,
         params.min_trimmed_reads
@@ -184,7 +186,7 @@ workflow SMRNASEQ {
         // Filter out sequences smaller than params.fastp_min_length
         FASTP_LENGTH_FILTER (
             UMITOOLS_EXTRACT.out.reads,
-            params.fastp_known_mirna_adapters,
+            mirna_adapters,
             params.save_trimmed_fail,
             params.save_merged
         )

From 18b6aa839f9f037d3a0f9a6e85734c2c2e5b0192 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 2 Feb 2024 14:42:41 +0000
Subject: [PATCH 164/227] change FASTP module publish dir names

---
 conf/modules.config | 12 ++++++------
 1 file changed, 6 insertions(+), 6 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 9c48f6f0..11c707a7 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -56,17 +56,17 @@ process {
         ].join(" ").trim()
         publishDir = [
             [
-                path: { "${params.outdir}/fastp/umi_extracted" },
+                path: { "${params.outdir}/fastp/on_raw" },
                 mode: params.publish_dir_mode,
                 pattern: "*.{json,html}"
             ],
             [
-                path: { "${params.outdir}/fastp/umi_extracted/log" },
+                path: { "${params.outdir}/fastp/on_raw/log" },
                 mode: params.publish_dir_mode,
                 pattern: "*.log"
             ],
             [
-                path: { "${params.outdir}/fastp/umi_extracted" },
+                path: { "${params.outdir}/fastp/on_raw" },
                 mode: params.publish_dir_mode,
                 pattern: "*.fail.fastq.gz",
                 enabled: params.save_trimmed_fail
@@ -106,17 +106,17 @@ process {
         ].join(" ").trim()
         publishDir = [
             [
-                path: { "${params.outdir}/fastp/length_filtered" },
+                path: { "${params.outdir}/fastp/on_umi_extracted" },
                 mode: params.publish_dir_mode,
                 pattern: "*.{json,html}"
             ],
             [
-                path: { "${params.outdir}/fastp/length_filtered/log" },
+                path: { "${params.outdir}/fastp/on_umi_extracted/log" },
                 mode: params.publish_dir_mode,
                 pattern: "*.log"
             ],
             [
-                path: { "${params.outdir}/fastp/length_filtered" },
+                path: { "${params.outdir}/fastp/on_umi_extracted" },
                 mode: params.publish_dir_mode,
                 pattern: "*.fail.fastq.gz",
                 enabled: params.save_trimmed_fail

From 59eea65947373c98b9a533696c356b88ac1538dd Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 2 Feb 2024 14:47:48 +0000
Subject: [PATCH 165/227] adapt default value of param fastp_max_length

---
 nextflow_schema.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 6e1b50a9..9a2f84a4 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -228,7 +228,7 @@
                 },
                 "fastp_max_length": {
                     "type": "integer",
-                    "default": 40,
+                    "default": 100,
                     "description": "Maximum filter length for raw reads.",
                     "fa_icon": "fas fa-ruler-horizontal"
                 },

From 5479ab070ad9bc04af079fb1126585d73700392b Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Mon, 5 Feb 2024 13:39:39 +0000
Subject: [PATCH 166/227] fix badges

---
 README.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/README.md b/README.md
index d7a978c4..2102a637 100644
--- a/README.md
+++ b/README.md
@@ -4,9 +4,11 @@
     <img alt="nf-core/smrnaseq" src="docs/images/nf-core-smrnaseq_logo_light.png">
   </picture>
 </h1>
+
 [![GitHub Actions CI Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/smrnaseq/actions?query=workflow%3A%22nf-core+CI%22)
 [![GitHub Actions Linting Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/smrnaseq/actions?query=workflow%3A%22nf-core+linting%22)
 [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/smrnaseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3456879-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3456879)
+
 [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)

From bde0ea22409b60f3a3b0e04d5c692a17365b54e9 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Mon, 5 Feb 2024 13:56:27 +0000
Subject: [PATCH 167/227] add sentence on three prime adapter default

---
 docs/usage.md | 6 ++++--
 1 file changed, 4 insertions(+), 2 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 94f3a61c..57e360b4 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -11,11 +11,13 @@
 This option indicates the experimental protocol used for the sample preparation. Currently supporting:
 
 - 'illumina': adapter (`TGGAATTCTCGGGTGCCAAGG`)
-- 'nextflex': adapter (`TGGAATTCTCGGGTGCCAAGG), clip_r1 (`4`), three_prime_clip_r1 (`4`)
+- 'nextflex': adapter (`TGGAATTCTCGGGTGCCAAGG`), clip_r1 (`4`), three_prime_clip_r1 (`4`)
 - 'qiaseq': adapter (`AACTGTAGGCACCATCAAT`)
-- 'cats': adapter (`GATCGGAAGAGCACACGTCTG), clip_r1(`3)
+- 'cats': adapter (`GATCGGAAGAGCACACGTCTG`), clip_r1(`3)
 - 'custom' (where the user can indicate the `three_prime_adapter`, `clip_r1` and `three_prime_clip_r1` manually)
 
+The parameter `--three_prime_adapter` is set to the Illumina adapter sequence `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`. This is also to ensure, that the auto-detect functionality of `FASTP` is not used. Please make sure to adapt this adapter sequence accordingly for your run.
+
 :warning: At least the `custom` protocol has to be specified, otherwise the pipeline won't run. In case you specify the `custom` protocol, ensure that the parameters above are set accordingly or the defaults will be applied. If you want to auto-detect the adapters using `fastp`, please set `--three_prime_adapter` to `""`.
 
 ### `mirtrace_species` or `mirgenedb_species`

From b6285a3632a8268b72bbe417a33672fe2491ae24 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Mon, 5 Feb 2024 14:14:32 +0000
Subject: [PATCH 168/227] add CATS kit adapter sequence

---
 assets/known_adapters.fa | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/assets/known_adapters.fa b/assets/known_adapters.fa
index 6b54be80..00999c05 100644
--- a/assets/known_adapters.fa
+++ b/assets/known_adapters.fa
@@ -4,3 +4,5 @@ AACTGTAGGCACCATCAAT
 TGGAATTCTCGGGTGCCAAGG
 > Nextflex miRNA adapter
 TGGAATTCTCGGGTGCCAAGG
+> CATS Small RNA-seq Kit adapter
+GATCGGAAGAGCACACGTCTG

From c50de92d7578acba37a3fd46612d699346149f05 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Mon, 5 Feb 2024 14:30:06 +0000
Subject: [PATCH 169/227] update docs on UMI parameters

---
 docs/usage.md | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 57e360b4..f5b733db 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -58,14 +58,14 @@ Contamination filtering of the sequencing reads is optional and can be invoked u
 
 ### UMI handling
 
-The pipeline handles UMIs with two tools `Umitools-extract` and subsequently `Umicollapse` to deduplicate using UMI information. This can be achieved by using the parameters for UMI handling:
+The pipeline handles UMIs with two tools `Umitools-extract` and subsequently `Umicollapse` to deduplicate using UMI information. This can be achieved by using the parameters for UMI handling as follows (in this case for the Qiagen kit):
 
 ```bash
---with_umi --umitools_bc_pattern = '.+AACTGTAGGCACCATCAAT{s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
+--with_umi --umitools_extract_method regex --umitools_bc_pattern = '.+(?P<discard_1>AACTGTAGGCACCATCAAT){s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
 ```
 
 :::note
-You will have to specify custom `umitools_bc_pattern` patterns if your UMI is different. Please check the required capability in your UMI handling manual.
+You will have to specify custom `umitools_bc_pattern` patterns if your UMI is different. Please check the required capability in your UMI handling manual. It should be set in a way, that only the sequence of the RNA molecule is left after extraction.
 :::
 
 ## Samplesheet input

From ece665ad33faa5f0c593637179975a6b6b26aad6 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Tue, 6 Feb 2024 08:29:48 +0000
Subject: [PATCH 170/227] improve usage docs on UMI

---
 docs/usage.md | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index f5b733db..b5eb01f0 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -16,7 +16,7 @@ This option indicates the experimental protocol used for the sample preparation.
 - 'cats': adapter (`GATCGGAAGAGCACACGTCTG`), clip_r1(`3)
 - 'custom' (where the user can indicate the `three_prime_adapter`, `clip_r1` and `three_prime_clip_r1` manually)
 
-The parameter `--three_prime_adapter` is set to the Illumina adapter sequence `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`. This is also to ensure, that the auto-detect functionality of `FASTP` is not used. Please make sure to adapt this adapter sequence accordingly for your run.
+The parameter `--three_prime_adapter` is set to the Illumina TruSeq single index adapter sequence `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`. This is also to ensure, that the auto-detect functionality of `FASTP` is disabled. Please make sure to adapt this adapter sequence accordingly for your run.
 
 :warning: At least the `custom` protocol has to be specified, otherwise the pipeline won't run. In case you specify the `custom` protocol, ensure that the parameters above are set accordingly or the defaults will be applied. If you want to auto-detect the adapters using `fastp`, please set `--three_prime_adapter` to `""`.
 
@@ -58,14 +58,14 @@ Contamination filtering of the sequencing reads is optional and can be invoked u
 
 ### UMI handling
 
-The pipeline handles UMIs with two tools `Umitools-extract` and subsequently `Umicollapse` to deduplicate using UMI information. This can be achieved by using the parameters for UMI handling as follows (in this case for the Qiagen kit):
+The pipeline handles UMIs with two tools. Umicollapse to deduplicate on entire read sequence after 3'adapter removal. Followed by Umitools-extract to extract the miRNA adapter and UMI. This can be achieved by using the parameters for UMI handling as follows (in this case for QIAseq miRNA Library Kit):
 
 ```bash
 --with_umi --umitools_extract_method regex --umitools_bc_pattern = '.+(?P<discard_1>AACTGTAGGCACCATCAAT){s<=2}(?P<umi_1>.{12})(?P<discard_2>.*)'
 ```
 
 :::note
-You will have to specify custom `umitools_bc_pattern` patterns if your UMI is different. Please check the required capability in your UMI handling manual. It should be set in a way, that only the sequence of the RNA molecule is left after extraction.
+You will have to specify custom umitools_bc_pattern patterns if your UMI read structure is different. Please check the required capability in your UMI handling manual. It should be set in a way, that only the insert sequence of the RNA molecule is left after extraction. Please refer to the manual of the used kit for the expected read structure.
 :::
 
 ## Samplesheet input

From a24fdcd9a85519dd5966ff34448144c65081a95a Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Tue, 6 Feb 2024 15:49:29 +0000
Subject: [PATCH 171/227] change parameter name to skip first umi extraction

---
 nextflow.config       | 15 ++++++++-------
 nextflow_schema.json  |  8 +++++---
 workflows/smrnaseq.nf |  2 +-
 3 files changed, 14 insertions(+), 11 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 34d26823..0e0e7a3a 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -30,13 +30,14 @@ params {
     mirgenedb_species           = null
 
     // UMI handling
-    with_umi                   = false
-    skip_umi_extract           = true //Needs to be true for fastq mode in collapsing reads
-    umitools_extract_method    = 'string'
-    umitools_bc_pattern        = null
-    umi_discard_read           = null
-    umitools_method            = 'dir'
-    save_umi_intermeds         = false
+    with_umi                      = false
+    // skips umitools extract in FASTQ_FASTQC_UMITOOLS_FASTP subworkflow. Needs to be true for fastq mode in collapsing reads
+    skip_umi_extract_before_dedup = true
+    umitools_extract_method       = 'string'
+    umitools_bc_pattern           = null
+    umi_discard_read              = null
+    umitools_method               = 'dir'
+    save_umi_intermeds            = false
 
     // Trimming options
     clip_r1                     = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 9a2f84a4..8c36246b 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -75,10 +75,11 @@
                     "fa_icon": "fas fa-layer-group",
                     "help_text": "Available options are dir, cc, adj"
                 },
-                "skip_umi_extract": {
+                "skip_umi_extract_before_dedup": {
                     "type": "boolean",
                     "fa_icon": "fas fa-compress-alt",
-                    "description": "Skip the UMI extraction from the read in case the UMIs have been moved to the headers in advance of the pipeline run."
+                    "description": "Skip the UMI extraction from the reads before deduplication. Please note, if this parameter is set to false, the reads will be deduplicated solely on insert sequence. UMIs might be extracted after deduplication depending on the set umitools_bc_pattern nevertheless if with_umi is set to True.",
+                    "default": true
                 },
                 "umitools_bc_pattern": {
                     "type": "string",
@@ -212,7 +213,8 @@
                 "three_prime_adapter": {
                     "type": "string",
                     "fa_icon": "fas fa-text-width",
-                    "description": "Sequencing adapter sequence to use for trimming."
+                    "description": "Sequencing adapter sequence to use for trimming.",
+                    "default": "AGATCGGAAGAGCACACGTCTGAACTCCAGTCA"
                 },
                 "trim_fastq": {
                     "type": "boolean",
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index f28f267a..21533ec6 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -143,7 +143,7 @@ workflow SMRNASEQ {
         ch_cat_fastq,
         params.skip_fastqc,
         params.with_umi,
-        params.skip_umi_extract,
+        params.skip_umi_extract_before_dedup,
         params.umi_discard_read,
         params.skip_fastp,
         mirna_adapters,

From 7af55aafb6df0ac5b57f9f585dda527c7936298e Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Tue, 6 Feb 2024 16:27:50 +0000
Subject: [PATCH 172/227] adapt pipeline overview to recent changes

---
 README.md | 25 +++++++++++++++----------
 1 file changed, 15 insertions(+), 10 deletions(-)

diff --git a/README.md b/README.md
index 2102a637..e49ec02b 100644
--- a/README.md
+++ b/README.md
@@ -35,19 +35,24 @@ You can find numerous talks on the nf-core events page from various topics inclu
 
 1. Quality check and triming
    1. Raw read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-   2. Adapter trimming ([`fastp`](https://github.com/OpenGene/fastp))
-   3. UMI barcode extraction ([`UMI-tools`](https://github.com/CGATOxford/UMI-tools))
-   4. Trim read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-2. miRNA QC ([`miRTrace`](https://github.com/friedlanderlab/mirtrace))
-3. Contamination filtering ([`Bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) (Optional)
+   2. UMI extraction and miRNA adapter trimming ([`UMI-tools`](https://github.com/CGATOxford/UMI-tools)) (Optional)
+   3. 3' adapter trimming ([`fastp`](https://github.com/OpenGene/fastp))
+   4. Read quality and length filter ([`fastp`](https://github.com/OpenGene/fastp))
+   5. Trim read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+2. UMI deduplication (Optional)
+   1. Deduplication on fastq-level ([`UMICollapse`](https://github.com/Daniel-Liu-c0deb0t/UMICollapse))
+   2. Barcode and miRNA adapter extraction ([`UMI-tools`](https://github.com/CGATOxford/UMI-tools))
+   3. Read length filter ([`fastp`](https://github.com/OpenGene/fastp))
+3. miRNA QC ([`miRTrace`](https://github.com/friedlanderlab/mirtrace))
+4. Contamination filtering ([`Bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) (Optional)
    1. rRNA filtration
    2. tRNA filtration
    3. cDNA filtration
    4. ncRNA filtration
    5. piRNA filtration
    6. Others filtration
-4. UMI barcode deduplication ([`UMI-tools`](https://github.com/CGATOxford/UMI-tools))
-5. miRNA quantification
+5. UMI barcode deduplication ([`UMI-tools`](https://github.com/CGATOxford/UMI-tools))
+6. miRNA quantification
    - EdgeR
      1. Reads alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
      2. Post-alignment processing of alignment against Mature miRNA ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
@@ -60,13 +65,13 @@ You can find numerous talks on the nf-core events page from various topics inclu
    - Mirtop quantification
      1. Read collapsing ([`seqcluster`](https://github.com/lpantano/seqcluster))
      2. miRNA and isomiR annotation ([`mirtop`](https://github.com/miRTop/mirtop))
-6. Genome Quantification (Optional)
+7. Genome Quantification (Optional)
    1. Reads alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml))
    2. Post-alignment processing of alignment against host reference genome ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/))
-7. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation)) (Optional)
+8. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation)) (Optional)
    1. Mapping against reference genome with the mapper module
    2. Known and novel miRNA discovery with the mirdeep2 module
-8. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
+9. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/))
 
 ## Usage
 

From 0c7400f735ee177a01a0c218b5ab29ab5e004da6 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Thu, 8 Feb 2024 16:22:28 +0000
Subject: [PATCH 173/227] add parameter to save aligned read optionally, use
 parameter to specify if merged reads should be stored

---
 conf/modules.config  |  5 +++--
 nextflow.config      |  4 ++--
 nextflow_schema.json | 11 +++++++++--
 3 files changed, 14 insertions(+), 6 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 11c707a7..5382a1dd 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -38,7 +38,7 @@ process {
             path: { "${params.outdir}/cat_fastq" },
             mode: params.publish_dir_mode,
             pattern: '*.fastq',
-            // enabled: params.save_merged_fastq //TODO ? implement save_merged_fastq param
+            enabled: params.save_merged
         ]
     }
 
@@ -317,7 +317,8 @@ process {
         publishDir = [
             path: { "${params.outdir}/genome_quant/bam" },
             mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+            enabled: params.save_aligned
         ]
     }
 
diff --git a/nextflow.config b/nextflow.config
index 34d26823..0382a4bf 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -28,6 +28,7 @@ params {
     mirgenedb_hairpin           = null
     mirgenedb_gff               = null
     mirgenedb_species           = null
+    save_aligned                = false
 
     // UMI handling
     with_umi                   = false
@@ -46,7 +47,7 @@ params {
     fastp_min_length            = 17
     fastp_known_mirna_adapters  = "$projectDir/assets/known_adapters.fa"
     save_trimmed_fail           = false
-    save_merged                 = false
+    save_merged                 = true
     skip_fastqc                 = false
     skip_multiqc                = false
     skip_mirdeep                = false
@@ -83,7 +84,6 @@ params {
     version                    = false
 
     // Config options
-
     custom_config_version       = 'master'
     custom_config_base          = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
     config_profile_description  = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 9a2f84a4..301f51a0 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -78,7 +78,8 @@
                 "skip_umi_extract": {
                     "type": "boolean",
                     "fa_icon": "fas fa-compress-alt",
-                    "description": "Skip the UMI extraction from the read in case the UMIs have been moved to the headers in advance of the pipeline run."
+                    "description": "Skip the UMI extraction from the read in case the UMIs have been moved to the headers in advance of the pipeline run.",
+                    "default": true
                 },
                 "umitools_bc_pattern": {
                     "type": "string",
@@ -184,6 +185,11 @@
                     "help_text": "Saving generated references means that you can use them for future pipeline runs, reducing processing times.",
                     "fa_icon": "fas fa-save"
                 },
+                "save_aligned": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-download",
+                    "description": "Save aligned reads in bam format."
+                },
                 "igenomes_ignore": {
                     "type": "boolean",
                     "description": "Do not load the iGenomes reference config.",
@@ -254,7 +260,8 @@
                 },
                 "save_merged": {
                     "type": "boolean",
-                    "description": "Save merged reads."
+                    "description": "Save merged reads.",
+                    "default": true
                 }
             }
         },

From 9b1994075adc3556c9a33a523eb0a4a067383738 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Thu, 8 Feb 2024 16:24:46 +0000
Subject: [PATCH 174/227] do not store aligned reads for test

---
 conf/test.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/test.config b/conf/test.config
index e699776b..f29eb50e 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -27,5 +27,6 @@ params {
     mirtrace_species = 'hsa'
     protocol         = 'illumina'
     skip_mirdeep     = true
+    save_aligned     = false
 
 }

From 3faa16c01cff141fdf9de57415c66f6385ea6d81 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 9 Feb 2024 07:55:39 +0000
Subject: [PATCH 175/227] set save_merged to false for test profile

---
 conf/test.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/test.config b/conf/test.config
index f29eb50e..94317d86 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -27,6 +27,6 @@ params {
     mirtrace_species = 'hsa'
     protocol         = 'illumina'
     skip_mirdeep     = true
-    save_aligned     = false
+    save_merged      = false
 
 }

From 598af9415e1c10084ad156bed9ea93cf5cb19082 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 9 Feb 2024 08:44:05 +0000
Subject: [PATCH 176/227] add parameter for specifying whether to save aligned
 reads from mirna quant subworkflow

---
 conf/modules.config  | 9 ++++++---
 nextflow.config      | 1 +
 nextflow_schema.json | 5 +++++
 3 files changed, 12 insertions(+), 3 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 5382a1dd..fb26908e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -230,7 +230,8 @@ process {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/mature" },
             mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+            enabled: params.save_aligned_mirna_quant
         ]
     }
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BAM_STATS_MATURE:.*' {
@@ -245,7 +246,8 @@ process {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/hairpin" },
             mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+            enabled: params.save_aligned_mirna_quant
         ]
     }
     withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BAM_STATS_HAIRPIN:.*' {
@@ -274,7 +276,8 @@ process {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/seqcluster" },
             mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+            enabled: params.save_aligned_mirna_quant
         ]
     }
     withName: 'MIRTOP_QUANT' {
diff --git a/nextflow.config b/nextflow.config
index 0382a4bf..850030a0 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -29,6 +29,7 @@ params {
     mirgenedb_gff               = null
     mirgenedb_species           = null
     save_aligned                = false
+    save_aligned_mirna_quant     = true
 
     // UMI handling
     with_umi                   = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 301f51a0..15df803b 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -190,6 +190,11 @@
                     "fa_icon": "fas fa-download",
                     "description": "Save aligned reads in bam format."
                 },
+                "save_aligned_mirna_quant": {
+                    "type": "boolean",
+                    "default": true,
+                    "description": "Save aligned reads in bam format of miRNA quant subworkflow."
+                },
                 "igenomes_ignore": {
                     "type": "boolean",
                     "description": "Do not load the iGenomes reference config.",

From 7d0a74939731565f0902f60cc409b97789a58a1b Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 9 Feb 2024 08:45:05 +0000
Subject: [PATCH 177/227] do not store aligned reads from mirna quant
 subworkflow in test

---
 conf/test.config | 9 +++++----
 1 file changed, 5 insertions(+), 4 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 94317d86..e826354b 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -24,9 +24,10 @@ params {
     input            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/samplesheet/v2.0/samplesheet.csv'
     fasta            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.fa'
 
-    mirtrace_species = 'hsa'
-    protocol         = 'illumina'
-    skip_mirdeep     = true
-    save_merged      = false
+    mirtrace_species         = 'hsa'
+    protocol                 = 'illumina'
+    skip_mirdeep             = true
+    save_merged              = false
+    save_aligned_mirna_quant = false
 
 }

From ab69e97406c276de197ca5c2aac731c5449584a1 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 9 Feb 2024 10:12:33 +0000
Subject: [PATCH 178/227] update CHANGELOG

---
 CHANGELOG.md | 25 +++++++++++++++----------
 1 file changed, 15 insertions(+), 10 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 8a4389cc..44dfcc63 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.3.0 - 2024-01-31 - Gray Zinc Dalmatian
+## v2.3.0 - 2024-02-09 - Gray Zinc Dalmatian
 
 - [[#307]](https://github.com/nf-core/smrnaseq/pull/307) - Clean up config file and improve output folder structure
 - [[#299]](https://github.com/nf-core/smrnaseq/issues/299) - Bugfix for missing inputs in BAM stats (`genome_quant.r`)
@@ -12,18 +12,23 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [[#294]](https://github.com/nf-core/smrnaseq/pull/294) - Fixed contamination screening issues
 - [[#309]](https://github.com/nf-core/smrnaseq/pull/309) - Merged in nf-core template v2.12.0
 - [[#310]](https://github.com/nf-core/smrnaseq/pull/310) - Removed unnecessarily separate mirtrace subworkflow, now using module instead
+- [[#311]](https://github.com/nf-core/smrnaseq/pull/311) - Fix use of FASTP, set `three_prime_adapter` per default
+- [[#314]](https://github.com/nf-core/smrnaseq/pull/314) - Add parameters to control publishing of intermediate results
 
 ### Parameters
 
-| Old parameter | New parameter               |
-| ------------- | --------------------------- |
-|               | `--with_umi`                |
-|               | `--umitools_extract_method` |
-|               | `--umitools_method`         |
-|               | `--skip_umi_extract`        |
-|               | `--umitools_bc_pattern`     |
-|               | `--umi_discard_read`        |
-|               | `--save_umi_intermeds`      |
+| Old parameter | New parameter                |
+| ------------- | ---------------------------- |
+|               | `--with_umi`                 |
+|               | `--umitools_extract_method`  |
+|               | `--umitools_method`          |
+|               | `--skip_umi_extract`         |
+|               | `--umitools_bc_pattern`      |
+|               | `--umi_discard_read`         |
+|               | `--save_umi_intermeds`       |
+|               | `--save_aligned`             |
+|               | `--save_aligned_mirna_quant` |
+|               | `--save_merged`              |
 
 ### Software dependencies
 

From 7ffb437aef839ca469f41675a8a743f60a597242 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 9 Feb 2024 10:24:01 +0000
Subject: [PATCH 179/227] schema help text, formatting

---
 nextflow.config      | 2 +-
 nextflow_schema.json | 9 ++++++---
 2 files changed, 7 insertions(+), 4 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 9f9e6b56..c5c0c2c6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -29,7 +29,7 @@ params {
     mirgenedb_gff               = null
     mirgenedb_species           = null
     save_aligned                = false
-    save_aligned_mirna_quant     = true
+    save_aligned_mirna_quant    = true
 
     // UMI handling
     with_umi                      = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index c3a65d71..4b78a7df 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -187,13 +187,16 @@
                 },
                 "save_aligned": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-download",
-                    "description": "Save aligned reads in bam format."
+                    "fa_icon": "fas fa-save",
+                    "help_text": "Save aligned reads of initial bowtie mapping.",
+                    "description": "Save aligned reads of initial mapping in bam format."
                 },
                 "save_aligned_mirna_quant": {
                     "type": "boolean",
+                    "fa_icon": "fas fa-save",
                     "default": true,
-                    "description": "Save aligned reads in bam format of miRNA quant subworkflow."
+                    "help_text": "Save aligned reads of the bowtie runs in BOWTIE_MAP_MATURE, BOWTIE_MAP_HAIRPIN, and BOWTIE_MAP_SEQCLUSTER.",
+                    "description": "Save aligned reads of miRNA quant subworkflow in bam format."
                 },
                 "igenomes_ignore": {
                     "type": "boolean",

From e35ff858da61293059c19f0d1a00fda6f43a0067 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Wed, 14 Feb 2024 13:11:41 +0000
Subject: [PATCH 180/227] add check if mirgene_db_species is set if mirgene_db

---
 workflows/smrnaseq.nf | 1 +
 1 file changed, 1 insertion(+)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 21533ec6..2ab3e507 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -62,6 +62,7 @@ if (!params.mirgenedb) {
     if (params.mirgenedb_mature) { reference_mature = file(params.mirgenedb_mature, checkIfExists: true) } else { exit 1, "Mature miRNA fasta file not found: ${params.mirgenedb_mature}" }
     if (params.mirgenedb_hairpin) { reference_hairpin = file(params.mirgenedb_hairpin, checkIfExists: true) } else { exit 1, "Hairpin miRNA fasta file not found: ${params.mirgenedb_hairpin}" }
     if (params.mirgenedb_gff) { mirna_gtf = file(params.mirgenedb_gff, checkIfExists: true) } else { exit 1, "MirGeneDB gff file not found: ${params.mirgenedb_gff}"}
+    if (!params.mirgenedb_species) { exit 1, "MirGeneDB species not set, please specify via the --mirgenedb_species parameter"}
 }
 
 include { INPUT_CHECK                               } from '../subworkflows/local/input_check'

From ab52f26409cccf86abca1fffecc8d34f1a69ac85 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 14 Feb 2024 17:07:25 +0000
Subject: [PATCH 181/227] Upgrade modules accordingly

---
 CHANGELOG.md                                  |   4 +-
 modules.json                                  |  22 +-
 modules/nf-core/fastp/tests/main.nf.test      |  30 +-
 modules/nf-core/fastp/tests/main.nf.test.snap | 257 ++++++++++++++++--
 modules/nf-core/multiqc/environment.yml       |   2 +-
 modules/nf-core/multiqc/main.nf               |   4 +-
 modules/nf-core/multiqc/tests/main.nf.test    |  13 +-
 .../nf-core/multiqc/tests/main.nf.test.snap   |  32 ++-
 .../nf-core/samtools/flagstat/environment.yml |   3 +-
 modules/nf-core/samtools/flagstat/main.nf     |   4 +-
 .../samtools/flagstat/tests/main.nf.test      |  12 +-
 .../samtools/flagstat/tests/main.nf.test.snap |  20 +-
 .../nf-core/samtools/idxstats/environment.yml |   3 +-
 modules/nf-core/samtools/idxstats/main.nf     |   4 +-
 .../samtools/idxstats/tests/main.nf.test      |  14 +-
 .../samtools/idxstats/tests/main.nf.test.snap |  20 +-
 .../nf-core/samtools/index/environment.yml    |   3 +-
 modules/nf-core/samtools/index/main.nf        |   4 +-
 .../nf-core/samtools/index/tests/main.nf.test |  36 +--
 .../samtools/index/tests/main.nf.test.snap    |  54 +++-
 modules/nf-core/samtools/sort/environment.yml |   3 +-
 modules/nf-core/samtools/sort/main.nf         |   4 +-
 .../nf-core/samtools/sort/tests/main.nf.test  |  33 +--
 .../samtools/sort/tests/main.nf.test.snap     |  47 +++-
 .../nf-core/samtools/stats/environment.yml    |   3 +-
 modules/nf-core/samtools/stats/main.nf        |   4 +-
 .../nf-core/samtools/stats/tests/main.nf.test |  53 ++--
 .../samtools/stats/tests/main.nf.test.snap    |  32 ++-
 .../umitools/extract/tests/main.nf.test       |   4 +-
 .../tests/main.nf.test.snap                   |  32 ++-
 .../tests/main.nf.test.snap                   |  48 +++-
 .../tests/main.nf.test.snap                   |  32 +++
 32 files changed, 629 insertions(+), 207 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 44dfcc63..3eb46248 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -34,14 +34,14 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 | Dependency    | Old version | New version |
 | ------------- | ----------- | ----------- |
-| `multiqc`     | 1.15        | 1.19        |
+| `multiqc`     | 1.15        | 1.20        |
 | `edgeR`       | 3.36.0      | 4.0.2       |
 | `limma`       | 3.50.0      | 3.58.1      |
 | `bioconvert`  | 0.4.3       | 1.1.1       |
 | `mirdeep`     | 2.0.1       | 2.0.1.3     |
 | `seqkit`      | 2.3.1       | 2.6.1       |
 | `fastqc`      | 0.11.4      | 0.12.1      |
-| `samtools`    | 1.17        | 1.18        |
+| `samtools`    | 1.17        | 1.19.2      |
 | `umitools`    | <none>      | 1.1.4       |
 | `umicollapse` | <none>      | 1.0.0       |
 
diff --git a/modules.json b/modules.json
index 168be565..abdf0255 100644
--- a/modules.json
+++ b/modules.json
@@ -22,7 +22,7 @@
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9",
+                        "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56",
                         "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fastqc": {
@@ -32,32 +32,32 @@
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
+                        "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290",
                         "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
-                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
                         "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
-                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
                         "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
-                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
                         "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
                         "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
-                        "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
                         "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "umicollapse": {
@@ -67,7 +67,7 @@
                     },
                     "umitools/extract": {
                         "branch": "master",
-                        "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53",
+                        "git_sha": "9e56d7a647fbf6f7e45ef123bc916ad66b6f7c9d",
                         "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     }
                 }
@@ -76,17 +76,17 @@
                 "nf-core": {
                     "bam_sort_stats_samtools": {
                         "branch": "master",
-                        "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
                         "installed_by": ["subworkflows"]
                     },
                     "bam_stats_samtools": {
                         "branch": "master",
-                        "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
                         "installed_by": ["bam_sort_stats_samtools", "subworkflows"]
                     },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
-                        "git_sha": "a4bceac1aecee5aa0a5dbc601baf0e2e61013fb2",
+                        "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test
index fa7e5b4d..9b3f9a38 100644
--- a/modules/nf-core/fastp/tests/main.nf.test
+++ b/modules/nf-core/fastp/tests/main.nf.test
@@ -67,9 +67,9 @@ nextflow_process {
                             process.out.reads_fail.collect { file(it[1]).getName() } +
                             process.out.reads_merged.collect { file(it[1]).getName() }
                         ).sort()
-                    ).match("test_fastp_single_end-for_stub_match")
+                    ).match("test_fastp_single_end-_match")
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_single_end") }
             )
         }
     }
@@ -116,7 +116,7 @@ nextflow_process {
                         ).sort()
                     ).match("test_fastp_single_end-for_stub_match")
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_single_end_stub") }
             )
         }
     }
@@ -196,9 +196,9 @@ nextflow_process {
                             process.out.reads_fail.collect { file(it[1]).getName() } +
                             process.out.reads_merged.collect { file(it[1]).getName() }
                         ).sort()
-                    ).match("test_fastp_paired_end-for_stub_match")
+                    ).match("test_fastp_paired_end_match")
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_paired_end") }
             )
         }
     }
@@ -245,7 +245,7 @@ nextflow_process {
                         ).sort()
                     ).match("test_fastp_paired_end-for_stub_match")
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_paired_end-stub") }
             )
         }
     }
@@ -310,9 +310,9 @@ nextflow_process {
                             process.out.reads_fail.collect { file(it[1]).getName() } +
                             process.out.reads_merged.collect { file(it[1]).getName() }
                         ).sort()
-                    ).match("test_fastp_interleaved-for_stub_match")
+                    ).match("test_fastp_interleaved-_match")
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_interleaved") }
             )
         }
     }
@@ -359,7 +359,7 @@ nextflow_process {
                         ).sort()
                     ).match("test_fastp_interleaved-for_stub_match")
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_interleaved-stub") }
             )
         }
     }
@@ -420,7 +420,7 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_single_end_trim_fail") }
             )
         }
     }
@@ -498,7 +498,7 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_paired_end_trim_fail") }
             )
         }
     }
@@ -587,9 +587,9 @@ nextflow_process {
                             process.out.reads_fail.collect { file(it[1]).getName() } +
                             process.out.reads_merged.collect { file(it[1]).getName() }
                         ).sort()
-                    ).match("test_fastp_paired_end_merged-for_stub_match")
+                    ).match("test_fastp_paired_end_merged_match")
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_paired_end_merged") }
             )
         }
     }
@@ -636,7 +636,7 @@ nextflow_process {
                         ).sort()
                     ).match("test_fastp_paired_end_merged-for_stub_match")
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_paired_end_merged_stub") }
             )
         }
     }
@@ -716,7 +716,7 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_paired_end_merged_adapterlist") }
             )
         }
     }
diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap
index 6a71b680..b4c0e1dd 100644
--- a/modules/nf-core/fastp/tests/main.nf.test.snap
+++ b/modules/nf-core/fastp/tests/main.nf.test.snap
@@ -1,19 +1,4 @@
 {
-    "test_fastp_paired_end-for_stub_match": {
-        "content": [
-            [
-                [
-                    "test_1.fastp.fastq.gz",
-                    "test_2.fastp.fastq.gz"
-                ],
-                "test.fastp.html",
-                "test.fastp.json",
-                "test.fastp.log",
-                "{id=test, single_end=false}"
-            ]
-        ],
-        "timestamp": "2024-01-17T18:07:15.398827"
-    },
     "fastp test_fastp_interleaved_json": {
         "content": [
             [
@@ -26,6 +11,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-17T18:08:06.123035"
     },
     "test_fastp_paired_end_merged-for_stub_match": {
@@ -42,8 +31,24 @@
                 "{id=test, single_end=false}"
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-17T18:10:13.467574"
     },
+    "versions_interleaved": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:56:24.615634793"
+    },
     "test_fastp_single_end_json": {
         "content": [
             [
@@ -56,15 +61,169 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-17T18:06:00.223817"
     },
-    "versions": {
+    "versions_paired_end": {
         "content": [
             [
                 "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
             ]
         ],
-        "timestamp": "2024-01-17T18:06:00.248422"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:55:42.333545689"
+    },
+    "test_fastp_paired_end_match": {
+        "content": [
+            [
+                [
+                    "test_1.fastp.fastq.gz",
+                    "test_2.fastp.fastq.gz"
+                ],
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=false}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T12:03:06.431833729"
+    },
+    "test_fastp_interleaved-_match": {
+        "content": [
+            [
+                "test.fastp.fastq.gz",
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=true}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T12:03:37.827323085"
+    },
+    "test_fastp_paired_end_merged_match": {
+        "content": [
+            [
+                [
+                    "test_1.fastp.fastq.gz",
+                    "test_2.fastp.fastq.gz"
+                ],
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "test.merged.fastq.gz",
+                "{id=test, single_end=false}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T12:08:44.496251446"
+    },
+    "versions_single_end_stub": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:55:27.354051299"
+    },
+    "versions_interleaved-stub": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:56:46.535528418"
+    },
+    "versions_single_end_trim_fail": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:59:03.724591407"
+    },
+    "test_fastp_paired_end-for_stub_match": {
+        "content": [
+            [
+                [
+                    "test_1.fastp.fastq.gz",
+                    "test_2.fastp.fastq.gz"
+                ],
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=false}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-17T18:07:15.398827"
+    },
+    "versions_paired_end-stub": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:56:06.50017282"
+    },
+    "versions_single_end": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:55:07.67921647"
+    },
+    "versions_paired_end_merged_stub": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:59:47.350653154"
     },
     "test_fastp_interleaved-for_stub_match": {
         "content": [
@@ -76,8 +235,24 @@
                 "{id=test, single_end=true}"
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-17T18:08:06.127974"
     },
+    "versions_paired_end_trim_fail": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:59:18.140484878"
+    },
     "test_fastp_single_end-for_stub_match": {
         "content": [
             [
@@ -88,8 +263,52 @@
                 "{id=test, single_end=true}"
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-17T18:06:00.244202"
     },
+    "test_fastp_single_end-_match": {
+        "content": [
+            [
+                "test.fastp.fastq.gz",
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=true}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:57:30.791982648"
+    },
+    "versions_paired_end_merged_adapterlist": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T12:05:37.845370554"
+    },
+    "versions_paired_end_merged": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:59:32.860543858"
+    },
     "test_fastp_single_end_trim_fail_json": {
         "content": [
             [
@@ -102,6 +321,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-17T18:08:41.942317"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index 7625b752..2212096a 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::multiqc=1.19
+  - bioconda::multiqc=1.20
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 1b9f7c43..354f4430 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.19--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.20--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
index d0438eda..f1c4242e 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -3,6 +3,7 @@ nextflow_process {
     name "Test Process MULTIQC"
     script "../main.nf"
     process "MULTIQC"
+
     tag "modules"
     tag "modules_nfcore"
     tag "multiqc"
@@ -12,7 +13,7 @@ nextflow_process {
         when {
             process {
                 """
-                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
                 input[1] = []
                 input[2] = []
                 input[3] = []
@@ -25,7 +26,7 @@ nextflow_process {
                 { assert process.success },
                 { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
                 { assert process.out.data[0] ==~ ".*/multiqc_data" },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("multiqc_versions_single") }
             )
         }
 
@@ -36,7 +37,7 @@ nextflow_process {
         when {
             process {
                 """
-                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
                 input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
                 input[2] = []
                 input[3] = []
@@ -49,7 +50,7 @@ nextflow_process {
                 { assert process.success },
                 { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
                 { assert process.out.data[0] ==~ ".*/multiqc_data" },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("multiqc_versions_config") }
             )
         }
     }
@@ -61,7 +62,7 @@ nextflow_process {
         when {
             process {
                 """
-                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
                 input[1] = []
                 input[2] = []
                 input[3] = []
@@ -75,7 +76,7 @@ nextflow_process {
                 { assert snapshot(process.out.report.collect { file(it).getName() } +
                                 process.out.data.collect { file(it).getName() } +
                                 process.out.plots.collect { file(it).getName() } +
-                                process.out.versions ).match() }
+                                process.out.versions ).match("multiqc_stub") }
             )
         }
 
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index d37e7304..c204b488 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -1,21 +1,41 @@
 {
-    "versions": {
+    "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d"
+                "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186"
             ]
         ],
-        "timestamp": "2024-01-09T23:02:49.911994"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-14T09:28:51.744211298"
     },
-    "sarscov2 single-end [fastqc] - stub": {
+    "multiqc_stub": {
         "content": [
             [
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d"
+                "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186"
             ]
         ],
-        "timestamp": "2024-01-09T23:03:14.524346"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-14T09:29:28.847433492"
+    },
+    "multiqc_versions_config": {
+        "content": [
+            [
+                "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-14T09:29:13.223621555"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/flagstat/environment.yml b/modules/nf-core/samtools/flagstat/environment.yml
index 5efae053..bd57cb54 100644
--- a/modules/nf-core/samtools/flagstat/environment.yml
+++ b/modules/nf-core/samtools/flagstat/environment.yml
@@ -4,4 +4,5 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.18
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf
index f1893d7c..eb5f5252 100644
--- a/modules/nf-core/samtools/flagstat/main.nf
+++ b/modules/nf-core/samtools/flagstat/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_FLAGSTAT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
-        'biocontainers/samtools:1.18--h50ea8bc_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test b/modules/nf-core/samtools/flagstat/tests/main.nf.test
index c8dd8dc9..24c3c04b 100644
--- a/modules/nf-core/samtools/flagstat/tests/main.nf.test
+++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test
@@ -16,11 +16,11 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
-                ]
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -28,8 +28,8 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert snapshot(process.out.flagstat).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+                { assert snapshot(process.out.flagstat).match("flagstat") },
+                { assert snapshot(process.out.versions).match("versions") }
             )
         }
     }
diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
index 880019f2..a76fc27e 100644
--- a/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "BAM": {
+    "flagstat": {
         "content": [
             [
                 [
@@ -11,6 +11,22 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-14T15:49:22.577133"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T18:31:37.783927"
+    },
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,fd0030ce49ab3a92091ad80260226452"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:11:44.299617452"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/idxstats/environment.yml b/modules/nf-core/samtools/idxstats/environment.yml
index 2401db0f..174973b8 100644
--- a/modules/nf-core/samtools/idxstats/environment.yml
+++ b/modules/nf-core/samtools/idxstats/environment.yml
@@ -4,4 +4,5 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.18
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf
index 00d916bb..a544026f 100644
--- a/modules/nf-core/samtools/idxstats/main.nf
+++ b/modules/nf-core/samtools/idxstats/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_IDXSTATS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
-        'biocontainers/samtools:1.18--h50ea8bc_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test b/modules/nf-core/samtools/idxstats/tests/main.nf.test
index f6c92150..a2dcb27c 100644
--- a/modules/nf-core/samtools/idxstats/tests/main.nf.test
+++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test
@@ -8,7 +8,7 @@ nextflow_process {
     tag "samtools"
     tag "samtools/idxstats"
 
-    test("BAM") {
+    test("bam") {
 
         when {
             params {
@@ -16,11 +16,11 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
-                ]
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -28,8 +28,8 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert snapshot(process.out.idxstats).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+                { assert snapshot(process.out.idxstats).match("idxstats") },
+                { assert snapshot(process.out.versions).match("versions") }
             )
         }
     }
diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
index 4c6c12bd..a7050bdc 100644
--- a/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
@@ -1,5 +1,17 @@
 {
-    "BAM": {
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,613dde56f108418039ffcdeeddba397a"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:16:50.147462763"
+    },
+    "idxstats": {
         "content": [
             [
                 [
@@ -11,6 +23,10 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-14T15:52:19.875194"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T18:36:41.561026"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml
index 296ed99e..a5e50649 100644
--- a/modules/nf-core/samtools/index/environment.yml
+++ b/modules/nf-core/samtools/index/environment.yml
@@ -4,4 +4,5 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.18
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf
index 8ad18fdc..dc14f98d 100644
--- a/modules/nf-core/samtools/index/main.nf
+++ b/modules/nf-core/samtools/index/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_INDEX {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
-        'biocontainers/samtools:1.18--h50ea8bc_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input)
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test
index c76a9169..bb7756d1 100644
--- a/modules/nf-core/samtools/index/tests/main.nf.test
+++ b/modules/nf-core/samtools/index/tests/main.nf.test
@@ -8,7 +8,7 @@ nextflow_process {
     tag "samtools"
     tag "samtools/index"
 
-    test("sarscov2 [BAI]") {
+    test("bai") {
 
         when {
             params {
@@ -16,10 +16,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -28,12 +28,12 @@ nextflow_process {
             assertAll (
                 { assert process.success },
                 { assert snapshot(process.out.bai).match("bai") },
-                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+                { assert snapshot(process.out.versions).match("bai_versions") }
             )
         }
     }
 
-    test("homo_sapiens [CRAI]") {
+    test("crai") {
 
         when {
             params {
@@ -41,10 +41,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true)
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -53,12 +53,12 @@ nextflow_process {
             assertAll (
                 { assert process.success },
                 { assert snapshot(process.out.crai).match("crai") },
-                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+                { assert snapshot(process.out.versions).match("crai_versions") }
             )
         }
     }
 
-    test("homo_sapiens [CSI]") {
+    test("csi") {
 
         config "./csi.nextflow.config"
 
@@ -68,10 +68,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -80,7 +80,7 @@ nextflow_process {
             assertAll (
                 { assert process.success },
                 { assert path(process.out.csi.get(0).get(1)).exists() },
-                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+                { assert snapshot(process.out.versions).match("csi_versions") }
             )
         }
     }
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap
index b3baee7f..3dc8e7de 100644
--- a/modules/nf-core/samtools/index/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap
@@ -1,28 +1,74 @@
 {
+    "crai_versions": {
+        "content": [
+            [
+                "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:12:00.324667957"
+    },
+    "csi_versions": {
+        "content": [
+            [
+                "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:12:07.885103162"
+    },
     "crai": {
         "content": [
             [
                 [
                     {
-                        "id": "test"
+                        "id": "test",
+                        "single_end": false
                     },
                     "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029"
                 ]
             ]
         ],
-        "timestamp": "2023-11-15T15:17:37.30801"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T18:41:38.446424"
     },
     "bai": {
         "content": [
             [
                 [
                     {
-                        "id": "test"
+                        "id": "test",
+                        "single_end": false
                     },
                     "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4"
                 ]
             ]
         ],
-        "timestamp": "2023-11-15T15:17:30.869234"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T18:40:46.579747"
+    },
+    "bai_versions": {
+        "content": [
+            [
+                "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:11:51.641425452"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/sort/environment.yml b/modules/nf-core/samtools/sort/environment.yml
index cd50868c..4d898e48 100644
--- a/modules/nf-core/samtools/sort/environment.yml
+++ b/modules/nf-core/samtools/sort/environment.yml
@@ -4,4 +4,5 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.18
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index 4a666d42..cdd8305d 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_SORT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
-        'biocontainers/samtools:1.18--h50ea8bc_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test
index abb80978..31e24b88 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test
@@ -8,7 +8,7 @@ nextflow_process {
     tag "samtools"
     tag "samtools/sort"
 
-    test("test_samtools_sort") {
+    test("bam") {
 
         config "./nextflow.config"
 
@@ -18,12 +18,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                    [ id:'test', single_end:false ],
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true)
-                    ]
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -34,10 +32,9 @@ nextflow_process {
                 { assert snapshot(process.out).match() }
             )
         }
-
     }
 
-    test("test_samtools_sort_stub") {
+    test("bam_stub") {
 
         config "./nextflow.config"
         options "-stub-run"
@@ -48,12 +45,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                    [ id:'test', single_end:false ],
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true)
-                    ]
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -61,13 +56,9 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert snapshot(
-                    file(process.out.bam[0][1]).name,
-                    process.out.versions
-                ).match() }
+                { assert snapshot(file(process.out.bam[0][1]).name).match("bam_stub_bam") },
+                { assert snapshot(process.out.versions).match("bam_stub_versions") }
             )
         }
-
     }
-
 }
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
index ff722259..a7cf0210 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
@@ -1,5 +1,27 @@
 {
-    "test_samtools_sort": {
+    "bam_stub_bam": {
+        "content": [
+            "test.sorted.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:21:04.364044"
+    },
+    "bam_stub_versions": {
+        "content": [
+            [
+                "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:15:00.20800281"
+    },
+    "bam": {
         "content": [
             {
                 "0": [
@@ -8,14 +30,14 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06"
+                        "test.sorted.bam:md5,c6ea1346ec4aae007eb40b708935088c"
                     ]
                 ],
                 "1": [
                     
                 ],
                 "2": [
-                    "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80"
+                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
                 ],
                 "bam": [
                     [
@@ -23,26 +45,21 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06"
+                        "test.sorted.bam:md5,c6ea1346ec4aae007eb40b708935088c"
                     ]
                 ],
                 "csi": [
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80"
+                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
                 ]
             }
         ],
-        "timestamp": "2023-12-04T11:11:22.005628301"
-    },
-    "test_samtools_sort_stub": {
-        "content": [
-            "test.sorted.bam",
-            [
-                "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80"
-            ]
-        ],
-        "timestamp": "2023-12-04T17:47:22.314445935"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:14:52.736359271"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml
index b89ce647..67bb0ca4 100644
--- a/modules/nf-core/samtools/stats/environment.yml
+++ b/modules/nf-core/samtools/stats/environment.yml
@@ -4,4 +4,5 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.18
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf
index 7539140a..52b00f4b 100644
--- a/modules/nf-core/samtools/stats/main.nf
+++ b/modules/nf-core/samtools/stats/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_STATS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' :
-        'biocontainers/samtools:1.18--h50ea8bc_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input), path(input_index)
diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test b/modules/nf-core/samtools/stats/tests/main.nf.test
index 20c3efe1..e3d5cb14 100644
--- a/modules/nf-core/samtools/stats/tests/main.nf.test
+++ b/modules/nf-core/samtools/stats/tests/main.nf.test
@@ -8,71 +8,58 @@ nextflow_process {
     tag "samtools"
     tag "samtools/stats"
 
-    test("SAMTOOLS STATS Should run without failures") {
+    test("bam") {
 
         when {
             params {
-                
                 outdir = "$outputDir"
             }
             process {
                 """
-                // define inputs of the process here.
-                input[0] = [ 
-                                [ id:'test', single_end:false ], // meta map
-                                file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
-                                file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
-                                
-                            ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
                 input[1] = [[],[]]
                 """
-
             }
         }
 
         then {
             assertAll(
-            {assert process.success},
-            {assert snapshot(process.out).match()}
+                {assert process.success},
+                {assert snapshot(process.out).match()}
             )
         }
-
     }
 
-    test("SAMTOOLS CRAM Should run without failures") {
+    test("cram") {
 
         when {
             params {
-                
                 outdir = "$outputDir"
             }
             process {
                 """
-                // define inputs of the process here
-                input[0] = [ 
-                                [ id:'test', single_end:false ], // meta map
-                                file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true),
-                                file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram_crai'], checkIfExists: true)
-                                
-                            ]
-                input[1] =  [ 
-                                [ id:'genome' ],
-                                file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
-                             ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
                 """
             }
-
-            
         }
 
         then {
             assertAll(
-            {assert process.success},
-            {assert snapshot(process.out).match()}
+                {assert process.success},
+                {assert snapshot(process.out).match()}
             )
         }
-
     }
-
-
 }
diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test.snap b/modules/nf-core/samtools/stats/tests/main.nf.test.snap
index 025c83a5..1b7c9ba4 100644
--- a/modules/nf-core/samtools/stats/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/stats/tests/main.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "SAMTOOLS STATS Should run without failures": {
+    "cram": {
         "content": [
             {
                 "0": [
@@ -8,11 +8,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def"
+                        "test.stats:md5,01812900aa4027532906c5d431114233"
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,650a365c6635001436008350ae83337c"
+                    "versions.yml:md5,0514ceb1769b2a88843e08c1f82624a9"
                 ],
                 "stats": [
                     [
@@ -20,17 +20,21 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def"
+                        "test.stats:md5,01812900aa4027532906c5d431114233"
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,650a365c6635001436008350ae83337c"
+                    "versions.yml:md5,0514ceb1769b2a88843e08c1f82624a9"
                 ]
             }
         ],
-        "timestamp": "2023-12-04T11:07:28.26821485"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:15:25.562429714"
     },
-    "SAMTOOLS CRAM Should run without failures": {
+    "bam": {
         "content": [
             {
                 "0": [
@@ -39,11 +43,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43"
+                        "test.stats:md5,5d8681bf541199898c042bf400391d59"
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,650a365c6635001436008350ae83337c"
+                    "versions.yml:md5,0514ceb1769b2a88843e08c1f82624a9"
                 ],
                 "stats": [
                     [
@@ -51,14 +55,18 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43"
+                        "test.stats:md5,5d8681bf541199898c042bf400391d59"
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,650a365c6635001436008350ae83337c"
+                    "versions.yml:md5,0514ceb1769b2a88843e08c1f82624a9"
                 ]
             }
         ],
-        "timestamp": "2023-12-04T11:07:50.356233402"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:15:07.857611509"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test b/modules/nf-core/umitools/extract/tests/main.nf.test
index 22242d1d..83a77a1b 100644
--- a/modules/nf-core/umitools/extract/tests/main.nf.test
+++ b/modules/nf-core/umitools/extract/tests/main.nf.test
@@ -18,8 +18,8 @@ nextflow_process {
             process {
                 """
                 input[0] = [ [ id:'test', single_end:true ], // meta map
-              [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
-            ]
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ]
+                ]
                 """
             }
         }
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
index c159eef3..6645a092 100644
--- a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
@@ -11,6 +11,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2023-10-22T20:25:03.687121177"
     },
     "test_bam_sort_stats_samtools_paired_end_idxstats": {
@@ -25,6 +29,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2023-10-22T20:25:03.709648916"
     },
     "test_bam_sort_stats_samtools_single_end_stats": {
@@ -35,11 +43,15 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.stats:md5,f281507081517414eb1a04b2d9c855b2"
+                    "test.stats:md5,cb0bf2b79de52fdf0c61e80efcdb0bb4"
                 ]
             ]
         ],
-        "timestamp": "2024-01-18T17:10:02.818694"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:44:38.553256801"
     },
     "test_bam_sort_stats_samtools_paired_end_stats": {
         "content": [
@@ -49,11 +61,15 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.stats:md5,e32e7e49dce1fbe327a89e0fb7bc01b1"
+                    "test.stats:md5,d7796222a087b9bb97f631f1c21b9c95"
                 ]
             ]
         ],
-        "timestamp": "2023-12-04T11:06:59.253905951"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:44:48.355870518"
     },
     "test_bam_sort_stats_samtools_single_end_idxstats": {
         "content": [
@@ -67,6 +83,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2024-01-18T17:10:02.84631"
     },
     "test_bam_sort_stats_samtools_single_end_flagstats": {
@@ -81,6 +101,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2024-01-18T17:10:02.829756"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
index 8bf0d379..bf0b0c69 100644
--- a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
@@ -11,6 +11,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2023-11-06T09:31:26.194017574"
     },
     "test_bam_stats_samtools_paired_end_stats": {
@@ -21,11 +25,15 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.stats:md5,49e2b43344ff92bc4c02463a58f7ba4a"
+                    "test.stats:md5,ddaf8f33fe9c1ebe9b06933213aec8ed"
                 ]
             ]
         ],
-        "timestamp": "2024-01-18T17:17:27.704335"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:45:06.230091746"
     },
     "test_bam_stats_samtools_paired_end_flagstats": {
         "content": [
@@ -39,6 +47,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2024-01-18T17:17:27.717482"
     },
     "test_bam_stats_samtools_single_end_flagstats": {
@@ -53,6 +65,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2023-11-06T09:26:10.340046381"
     },
     "test_bam_stats_samtools_paired_end_cram_idxstats": {
@@ -67,6 +83,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2023-11-06T09:31:26.207052003"
     },
     "test_bam_stats_samtools_single_end_stats": {
@@ -77,11 +97,15 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.stats:md5,5a6667d97806e5002731e9cf23674fad"
+                    "test.stats:md5,dc178e1a4956043aba8abc83e203521b"
                 ]
             ]
         ],
-        "timestamp": "2023-12-04T11:07:06.676820877"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:44:57.442208382"
     },
     "test_bam_stats_samtools_paired_end_idxstats": {
         "content": [
@@ -95,6 +119,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2024-01-18T17:17:27.726719"
     },
     "test_bam_stats_samtools_single_end_idxstats": {
@@ -109,6 +137,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
         "timestamp": "2023-11-06T09:26:10.349439801"
     },
     "test_bam_stats_samtools_paired_end_cram_stats": {
@@ -119,10 +151,14 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.stats:md5,2cf2fe93596ee3d74f946097b204a629"
+                    "test.stats:md5,d3345c4887f4a9ea4f7f56405b495db0"
                 ]
             ]
         ],
-        "timestamp": "2023-12-04T11:07:22.30295557"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:45:14.997164209"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
index 973746a3..89ba8da1 100644
--- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
@@ -5,6 +5,10 @@
                 
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-12T08:38:50.041635573"
     },
     "trim_reads_fail": {
@@ -13,6 +17,10 @@
                 
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-12T08:38:50.033284693"
     },
     "versions": {
@@ -23,6 +31,10 @@
                 "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-12T08:38:50.121510557"
     },
     "trim_json": {
@@ -37,6 +49,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-12T08:38:50.024410724"
     },
     "adapter_seq": {
@@ -51,6 +67,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-12T08:38:50.08674429"
     },
     "reads": {
@@ -68,6 +88,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-12T08:38:49.994419936"
     },
     "umi_log": {
@@ -76,6 +100,10 @@
                 
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-12T08:38:50.017720214"
     },
     "trim_read_count": {
@@ -90,6 +118,10 @@
                 ]
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-12T08:38:50.102326089"
     }
 }
\ No newline at end of file

From c0293f1907fc3ee7038d7f2fc80413ce81bf7e12 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 15 Feb 2024 08:14:43 +0000
Subject: [PATCH 182/227] Cleanup docker config

---
 nextflow.config | 2 --
 1 file changed, 2 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index c5c0c2c6..8af960f8 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -164,8 +164,6 @@ profiles {
     }
     docker {
         docker.enabled         = true
-        docker.userEmulation   = true
-        docker.runOptions      = '-u $(id -u):$(id -g)'
         conda.enabled          = false
         singularity.enabled    = false
         podman.enabled         = false

From 752386562abe9642ce852faf4e620af376faf5c4 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 15 Feb 2024 08:45:13 +0000
Subject: [PATCH 183/227] Tiny update on modules to make tests pass

---
 modules.json                                                | 2 +-
 modules/nf-core/custom/dumpsoftwareversions/environment.yml | 2 +-
 modules/nf-core/custom/dumpsoftwareversions/main.nf         | 4 ++--
 3 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules.json b/modules.json
index abdf0255..b7261f61 100644
--- a/modules.json
+++ b/modules.json
@@ -17,7 +17,7 @@
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
+                        "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1",
                         "installed_by": ["modules"]
                     },
                     "fastp": {
diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
index 9b3272bc..b48ced26 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::multiqc=1.19
+  - bioconda::multiqc=1.20
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index f2187611..105f9265 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.19--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.20--pyhdfd78af_0' }"
 
     input:
     path versions

From 2703dfa7ea447e55bafab1be44f53e750776e1c8 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 15 Feb 2024 10:05:36 +0000
Subject: [PATCH 184/227] Add tests clean

---
 conf/test.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/test.config b/conf/test.config
index e826354b..a56b2e96 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -30,4 +30,5 @@ params {
     save_merged              = false
     save_aligned_mirna_quant = false
 
+    cleanup                  = true //Otherwise tests dont run through properly.
 }

From 607796b296175067e0f2350969d86c6c4eae5247 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 15 Feb 2024 12:50:48 +0000
Subject: [PATCH 185/227] Try to clean up first

---
 .github/workflows/ci.yml | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 51c3ee37..e7ec0a27 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -39,6 +39,9 @@ jobs:
         with:
           version: "${{ matrix.NXF_VER }}"
 
+      - name: Free Disk Space (Ubuntu)
+        uses: jlumbroso/free-disk-space@v1.3.1
+
       - name: Run pipeline with test data
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.profile }},docker --outdir ./results

From cbee5e700d6bfc70b3ac6f6195e1cfaa72ec937c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 15 Feb 2024 13:15:36 +0000
Subject: [PATCH 186/227] Science

---
 .github/workflows/ci.yml | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index e7ec0a27..903f1b03 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -45,3 +45,6 @@ jobs:
       - name: Run pipeline with test data
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.profile }},docker --outdir ./results
+
+      - name: Free Disk Space for Phil - Because Science!
+        uses: jlumbroso/free-disk-space@v1.3.1

From 84c42d4df3a057545e63062565820f75dc350f01 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 16 Feb 2024 08:16:36 +0000
Subject: [PATCH 187/227] fix index ref and fasta init

---
 workflows/smrnaseq.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 21533ec6..2527f98e 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -153,7 +153,7 @@ workflow SMRNASEQ {
     )
     ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
 
-    ch_fasta = params.fasta ? file(params.fasta): []
+    ch_fasta = params.fasta ? Channel.value(file(params.fasta)) : []
     ch_reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
 
     // even if bowtie index is specified, there still needs to be a fasta.
@@ -162,7 +162,7 @@ workflow SMRNASEQ {
         //Prepare bowtie index, unless specified
         //This needs to be done here as the index is used by GENOME_QUANT
         if(params.bowtie_index) {
-            ch_bowtie_index  = Channel.fromPath("${index}**ebwt", checkIfExists: true).ifEmpty { error "Bowtie1 index directory not found: ${index}" }
+            Channel.fromPath("${params.bowtie_index}**ebwt", checkIfExists: true).ifEmpty{ error "Bowtie1 index directory not found: ${index}" }.set { ch_bowtie_index }
         } else {
             INDEX_GENOME ( [ [:], ch_fasta ] )
             ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)

From 14e08c17ba98a748d95dd056a75ad7b9559d5cac Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 16 Feb 2024 08:35:41 +0000
Subject: [PATCH 188/227] revert fasta channel change

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 2527f98e..9b3d66aa 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -153,7 +153,7 @@ workflow SMRNASEQ {
     )
     ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
 
-    ch_fasta = params.fasta ? Channel.value(file(params.fasta)) : []
+    ch_fasta = params.fasta ? file(params.fasta) : []
     ch_reads_for_mirna = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
 
     // even if bowtie index is specified, there still needs to be a fasta.

From 0bf058afc02ee7cc7bb242616063b01072d28cfe Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 16 Feb 2024 09:13:40 +0000
Subject: [PATCH 189/227] use main smrnaseq test data branch for test

---
 conf/test_no_genome.config | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/conf/test_no_genome.config b/conf/test_no_genome.config
index 485870de..aae8ce91 100644
--- a/conf/test_no_genome.config
+++ b/conf/test_no_genome.config
@@ -21,9 +21,9 @@ params {
 
     // Input data
     input            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/samplesheet/v2.0/samplesheet.csv'
-    mature           = 'https://github.com/nf-core/test-datasets/raw/smrnaseq-better-input/reference/mature.fa'
-    hairpin          = 'https://github.com/nf-core/test-datasets/raw/smrnaseq-better-input/reference/hairpin.fa'
-    mirna_gtf        = 'https://github.com/nf-core/test-datasets/raw/smrnaseq-better-input/reference/hsa.gff3'
+    mature           = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/mature.fa'
+    hairpin          = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hairpin.fa'
+    mirna_gtf        = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hsa.gff3'
     mirtrace_species = 'hsa'
     skip_mirdeep     = true
     protocol         = 'illumina'

From fff13659c2d63d8f272fc75feed771b075d9eff1 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 16 Feb 2024 09:19:05 +0000
Subject: [PATCH 190/227] add test profile for testing provided bowtie index

---
 .github/workflows/ci.yml |  1 +
 conf/test_index.config   | 35 +++++++++++++++++++++++++++++++++++
 2 files changed, 36 insertions(+)
 create mode 100644 conf/test_index.config

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 903f1b03..55ae6171 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -30,6 +30,7 @@ jobs:
           - "test"
           - "test_no_genome"
           - "test_umi"
+          - "test_index"
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v4
diff --git a/conf/test_index.config b/conf/test_index.config
new file mode 100644
index 00000000..64ae5766
--- /dev/null
+++ b/conf/test_index.config
@@ -0,0 +1,35 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for running minimal tests
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines input files and everything required to run a fast and simple pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/smrnaseq -profile test_index,<docker/singularity> --outdir <OUTDIR>
+
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    config_profile_name        = 'Test index profile'
+    config_profile_description = 'Minimal test dataset to check pipeline function with bowtie index'
+
+    // Limit resources so that this can run on GitHub Actions
+    max_cpus   = 2
+    max_memory = '6.GB'
+    max_time   = '6.h'
+
+    // Input data
+
+    input            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/samplesheet/v2.0/samplesheet.csv'
+    fasta            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.fa'
+    bowtie_index     = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/'
+
+    mirtrace_species         = 'hsa'
+    protocol                 = 'illumina'
+    skip_mirdeep             = true
+    save_merged              = false
+    save_aligned_mirna_quant = false
+
+    cleanup                  = true //Otherwise tests dont run through properly.
+}

From c951cc1b46af51deae6e4e26a8a2e9a42e8f020c Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 16 Feb 2024 09:31:48 +0000
Subject: [PATCH 191/227] add new test profile to config

---
 nextflow.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/nextflow.config b/nextflow.config
index 8af960f8..0f802b0b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -233,6 +233,7 @@ profiles {
     test_umi       { includeConfig 'conf/test_umi.config' }
     test_no_genome { includeConfig 'conf/test_no_genome.config' }
     test_full      { includeConfig 'conf/test_full.config' }
+    test_index     { includeConfig 'conf/test_index.config' }
 }
 
 // Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile

From 0074162b4ff14c1f3160e871803e922270afc1f1 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Fri, 16 Feb 2024 09:42:56 +0000
Subject: [PATCH 192/227] replace index variable

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 9b3d66aa..c509d59c 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -162,7 +162,7 @@ workflow SMRNASEQ {
         //Prepare bowtie index, unless specified
         //This needs to be done here as the index is used by GENOME_QUANT
         if(params.bowtie_index) {
-            Channel.fromPath("${params.bowtie_index}**ebwt", checkIfExists: true).ifEmpty{ error "Bowtie1 index directory not found: ${index}" }.set { ch_bowtie_index }
+            Channel.fromPath("${params.bowtie_index}**ebwt", checkIfExists: true).ifEmpty{ error "Bowtie1 index directory not found: ${params.bowtie_index}" }.set { ch_bowtie_index }
         } else {
             INDEX_GENOME ( [ [:], ch_fasta ] )
             ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)

From 134d266f8848248ca21d53997defc8fa035874bb Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Mon, 19 Feb 2024 15:41:25 +0000
Subject: [PATCH 193/227] initialize fasta channel depending on bowtie_index

---
 workflows/smrnaseq.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index c509d59c..d86b0280 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -162,7 +162,8 @@ workflow SMRNASEQ {
         //Prepare bowtie index, unless specified
         //This needs to be done here as the index is used by GENOME_QUANT
         if(params.bowtie_index) {
-            Channel.fromPath("${params.bowtie_index}**ebwt", checkIfExists: true).ifEmpty{ error "Bowtie1 index directory not found: ${params.bowtie_index}" }.set { ch_bowtie_index }
+            Channel.fromPath("${params.bowtie_index}**ebwt", checkIfExists: true).ifEmpty{ error "Bowtie1 index directory not found: ${params.bowtie_index}" }.filter { it != null }.set { ch_bowtie_index }
+            ch_fasta = Channel.fromPath(params.fasta)
         } else {
             INDEX_GENOME ( [ [:], ch_fasta ] )
             ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)

From a22b41b21c4383135ecec629a2840a3c1c650d20 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 20 Feb 2024 15:30:46 +0000
Subject: [PATCH 194/227] Template update for nf-core/tools version 2.13

---
 .editorconfig                                 |   9 +-
 .github/workflows/awsfulltest.yml             |   4 +-
 .github/workflows/awstest.yml                 |   4 +-
 .github/workflows/branch.yml                  |   2 +-
 .github/workflows/ci.yml                      |   7 +-
 .github/workflows/clean-up.yml                |   2 +-
 .github/workflows/download_pipeline.yml       |  17 +-
 .github/workflows/linting.yml                 |  12 +-
 .github/workflows/linting_comment.yml         |   4 +-
 .github/workflows/release-announcements.yml   |  11 +-
 README.md                                     |   5 +-
 assets/multiqc_config.yml                     |   2 +
 assets/schema_input.json                      |  21 +-
 bin/check_samplesheet.py                      | 259 -----------
 conf/modules.config                           |   8 -
 lib/NfcoreTemplate.groovy                     | 356 --------------
 lib/Utils.groovy                              |  47 --
 lib/WorkflowMain.groovy                       |  77 ---
 lib/WorkflowSmrnaseq.groovy                   | 122 -----
 main.nf                                       | 100 ++--
 modules.json                                  |  28 +-
 modules/local/samplesheet_check.nf            |  31 --
 .../dumpsoftwareversions/environment.yml      |   7 -
 .../custom/dumpsoftwareversions/main.nf       |  24 -
 .../custom/dumpsoftwareversions/meta.yml      |  37 --
 .../templates/dumpsoftwareversions.py         | 102 ----
 .../dumpsoftwareversions/tests/main.nf.test   |  43 --
 .../tests/main.nf.test.snap                   |  33 --
 .../dumpsoftwareversions/tests/tags.yml       |   2 -
 modules/nf-core/fastqc/tests/main.nf.test     |  14 +-
 .../nf-core/fastqc/tests/main.nf.test.snap    |  76 ++-
 modules/nf-core/multiqc/environment.yml       |   2 +-
 modules/nf-core/multiqc/main.nf               |   4 +-
 modules/nf-core/multiqc/tests/main.nf.test    |  13 +-
 .../nf-core/multiqc/tests/main.nf.test.snap   |  32 +-
 nextflow.config                               |   5 +-
 nextflow_schema.json                          |   1 +
 pyproject.toml                                |   8 +-
 subworkflows/local/input_check.nf             |  44 --
 .../utils_nfcore_smrnaseq_pipeline/main.nf    | 247 ++++++++++
 .../nf-core/utils_nextflow_pipeline/main.nf   | 126 +++++
 .../nf-core/utils_nextflow_pipeline/meta.yml  |  38 ++
 .../tests/main.function.nf.test               |  54 +++
 .../tests/main.function.nf.test.snap          |  12 +
 .../tests/main.workflow.nf.test               | 123 +++++
 .../tests/nextflow.config                     |   9 +
 .../utils_nextflow_pipeline/tests/tags.yml    |   2 +
 .../nf-core/utils_nfcore_pipeline/main.nf     | 440 ++++++++++++++++++
 .../nf-core/utils_nfcore_pipeline/meta.yml    |  24 +
 .../tests/main.function.nf.test               | 134 ++++++
 .../tests/main.function.nf.test.snap          | 138 ++++++
 .../tests/main.workflow.nf.test               |  29 ++
 .../tests/main.workflow.nf.test.snap          |  15 +
 .../tests/nextflow.config                     |   9 +
 .../utils_nfcore_pipeline/tests/tags.yml      |   2 +
 .../nf-core/utils_nfvalidation_plugin/main.nf |  62 +++
 .../utils_nfvalidation_plugin/meta.yml        |  44 ++
 .../tests/main.nf.test                        | 200 ++++++++
 .../tests/nextflow_schema.json                |  96 ++++
 .../utils_nfvalidation_plugin/tests/tags.yml  |   2 +
 workflows/smrnaseq.nf                         | 133 ++----
 61 files changed, 2108 insertions(+), 1406 deletions(-)
 delete mode 100755 bin/check_samplesheet.py
 delete mode 100755 lib/NfcoreTemplate.groovy
 delete mode 100644 lib/Utils.groovy
 delete mode 100755 lib/WorkflowMain.groovy
 delete mode 100755 lib/WorkflowSmrnaseq.groovy
 delete mode 100644 modules/local/samplesheet_check.nf
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml
 delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
 delete mode 100644 subworkflows/local/input_check.nf
 create mode 100644 subworkflows/local/utils_nfcore_smrnaseq_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
 create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml

diff --git a/.editorconfig b/.editorconfig
index 9b990088..dd9ffa53 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -18,7 +18,12 @@ end_of_line = unset
 insert_final_newline = unset
 trim_trailing_whitespace = unset
 indent_style = unset
-indent_size = unset
+[/subworkflows/nf-core/**]
+charset = unset
+end_of_line = unset
+insert_final_newline = unset
+trim_trailing_whitespace = unset
+indent_style = unset
 
 [/assets/email*]
 indent_size = unset
@@ -28,5 +33,5 @@ indent_size = unset
 indent_style = unset
 
 # ignore python
-[*.{py}]
+[*.{py,md}]
 indent_style = unset
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index b849392a..3c8cf6d6 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -14,7 +14,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Launch workflow via tower
-        uses: seqeralabs/action-tower-launch@v2
+        uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2
         # TODO nf-core: You can customise AWS full pipeline tests as required
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
@@ -31,7 +31,7 @@ jobs:
             }
           profiles: test_full
 
-      - uses: actions/upload-artifact@v4
+      - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4
         with:
           name: Tower debug log file
           path: |
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 5386cbc0..3ed287c5 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -12,7 +12,7 @@ jobs:
     steps:
       # Launch workflow using Tower CLI tool action
       - name: Launch workflow via tower
-        uses: seqeralabs/action-tower-launch@v2
+        uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
@@ -25,7 +25,7 @@ jobs:
             }
           profiles: test
 
-      - uses: actions/upload-artifact@v4
+      - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4
         with:
           name: Tower debug log file
           path: |
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index e6f6941e..5deaba55 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -19,7 +19,7 @@ jobs:
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
       - name: Post PR comment
         if: failure()
-        uses: mshick/add-pr-comment@v2
+        uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
         with:
           message: |
             ## This PR is against the `master` branch :x:
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 60f93b48..5bd73b88 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -28,13 +28,16 @@ jobs:
           - "latest-everything"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v4
+        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1
         with:
           version: "${{ matrix.NXF_VER }}"
 
+      - name: Disk space cleanup
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+
       - name: Run pipeline with test data
         # TODO nf-core: You can customise CI pipeline run tests as required
         # For example: adding multiple test runs with different parameters
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
index e37cfda5..0b6b1f27 100644
--- a/.github/workflows/clean-up.yml
+++ b/.github/workflows/clean-up.yml
@@ -10,7 +10,7 @@ jobs:
       issues: write
       pull-requests: write
     steps:
-      - uses: actions/stale@v9
+      - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9
         with:
           stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
           stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 8611458a..f823210d 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download'
 #  - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev.
 on:
   workflow_dispatch:
+    inputs:
+      testbranch:
+        description: "The specific branch you wish to utilize for the test execution of nf-core download."
+        required: true
+        default: "dev"
   pull_request:
     types:
       - opened
@@ -23,13 +28,13 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1
 
-      - uses: actions/setup-python@v5
+      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
         with:
           python-version: "3.11"
           architecture: "x64"
-      - uses: eWaterCycle/setup-singularity@v7
+      - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7
         with:
           singularity-version: 3.8.3
 
@@ -42,13 +47,13 @@ jobs:
         run: |
           echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV}
           echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV}
-          echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV}
+          echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV}
 
       - name: Download the pipeline
         env:
           NXF_SINGULARITY_CACHEDIR: ./
         run: |
-          nf-core download  ${{ env.REPO_LOWERCASE }} \
+          nf-core download ${{ env.REPO_LOWERCASE }} \
           --revision ${{ env.REPO_BRANCH }} \
           --outdir ./${{ env.REPOTITLE_LOWERCASE }} \
           --compress "none" \
@@ -64,4 +69,4 @@ jobs:
         env:
           NXF_SINGULARITY_CACHEDIR: ./
           NXF_SINGULARITY_HOME_MOUNT: true
-        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results 
+        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 81cd098e..748b4311 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,10 +14,10 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
       - name: Set up Python 3.11
-        uses: actions/setup-python@v5
+        uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
         with:
           python-version: 3.11
           cache: "pip"
@@ -32,12 +32,12 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v4
+        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1
 
-      - uses: actions/setup-python@v5
+      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
         with:
           python-version: "3.11"
           architecture: "x64"
@@ -60,7 +60,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@v4
+        uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 147bcd10..b706875f 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@v3
+        uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3
         with:
           workflow: linting.yml
           workflow_conclusion: completed
@@ -21,7 +21,7 @@ jobs:
         run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT
 
       - name: Post PR comment
-        uses: marocchino/sticky-pull-request-comment@v2
+        uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2
         with:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 21ac3f06..c3674af2 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -9,6 +9,11 @@ jobs:
   toot:
     runs-on: ubuntu-latest
     steps:
+      - name: get topics and convert to hashtags
+        id: get_topics
+        run: |
+          curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT
+
       - uses: rzr/fediverse-action@master
         with:
           access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }}
@@ -20,11 +25,13 @@ jobs:
 
             Please see the changelog: ${{ github.event.release.html_url }}
 
+            ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics
+
   send-tweet:
     runs-on: ubuntu-latest
 
     steps:
-      - uses: actions/setup-python@v5
+      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
         with:
           python-version: "3.10"
       - name: Install dependencies
@@ -56,7 +63,7 @@ jobs:
   bsky-post:
     runs-on: ubuntu-latest
     steps:
-      - uses: zentered/bluesky-post-action@v0.1.0
+      - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0
         with:
           post: |
             Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
diff --git a/README.md b/README.md
index 1095a9f3..e38215dd 100644
--- a/README.md
+++ b/README.md
@@ -4,8 +4,9 @@
     <img alt="nf-core/smrnaseq" src="docs/images/nf-core-smrnaseq_logo_light.png">
   </picture>
 </h1>
-[![GitHub Actions CI Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/smrnaseq/actions?query=workflow%3A%22nf-core+CI%22)
-[![GitHub Actions Linting Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/smrnaseq/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/smrnaseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
+
+[![GitHub Actions CI Status](https://github.com/nf-core/smrnaseq/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/smrnaseq/actions/workflows/ci.yml)
+[![GitHub Actions Linting Status](https://github.com/nf-core/smrnaseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/smrnaseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/smrnaseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 
 [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 77215840..c2cfbb98 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -13,3 +13,5 @@ report_section_order:
     order: -1002
 
 export_plots: true
+
+disable_version_detection: true
diff --git a/assets/schema_input.json b/assets/schema_input.json
index 5041ee60..892b1996 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -10,25 +10,22 @@
             "sample": {
                 "type": "string",
                 "pattern": "^\\S+$",
-                "errorMessage": "Sample name must be provided and cannot contain spaces"
+                "errorMessage": "Sample name must be provided and cannot contain spaces",
+                "meta": ["id"]
             },
             "fastq_1": {
                 "type": "string",
+                "format": "file-path",
+                "exists": true,
                 "pattern": "^\\S+\\.f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "fastq_2": {
-                "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'",
-                "anyOf": [
-                    {
-                        "type": "string",
-                        "pattern": "^\\S+\\.f(ast)?q\\.gz$"
-                    },
-                    {
-                        "type": "string",
-                        "maxLength": 0
-                    }
-                ]
+                "type": "string",
+                "format": "file-path",
+                "exists": true,
+                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
+                "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             }
         },
         "required": ["sample", "fastq_1"]
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
deleted file mode 100755
index 4a758fe0..00000000
--- a/bin/check_samplesheet.py
+++ /dev/null
@@ -1,259 +0,0 @@
-#!/usr/bin/env python
-
-
-"""Provide a command line tool to validate and transform tabular samplesheets."""
-
-
-import argparse
-import csv
-import logging
-import sys
-from collections import Counter
-from pathlib import Path
-
-logger = logging.getLogger()
-
-
-class RowChecker:
-    """
-    Define a service that can validate and transform each given row.
-
-    Attributes:
-        modified (list): A list of dicts, where each dict corresponds to a previously
-            validated and transformed row. The order of rows is maintained.
-
-    """
-
-    VALID_FORMATS = (
-        ".fq.gz",
-        ".fastq.gz",
-    )
-
-    def __init__(
-        self,
-        sample_col="sample",
-        first_col="fastq_1",
-        second_col="fastq_2",
-        single_col="single_end",
-        **kwargs,
-    ):
-        """
-        Initialize the row checker with the expected column names.
-
-        Args:
-            sample_col (str): The name of the column that contains the sample name
-                (default "sample").
-            first_col (str): The name of the column that contains the first (or only)
-                FASTQ file path (default "fastq_1").
-            second_col (str): The name of the column that contains the second (if any)
-                FASTQ file path (default "fastq_2").
-            single_col (str): The name of the new column that will be inserted and
-                records whether the sample contains single- or paired-end sequencing
-                reads (default "single_end").
-
-        """
-        super().__init__(**kwargs)
-        self._sample_col = sample_col
-        self._first_col = first_col
-        self._second_col = second_col
-        self._single_col = single_col
-        self._seen = set()
-        self.modified = []
-
-    def validate_and_transform(self, row):
-        """
-        Perform all validations on the given row and insert the read pairing status.
-
-        Args:
-            row (dict): A mapping from column headers (keys) to elements of that row
-                (values).
-
-        """
-        self._validate_sample(row)
-        self._validate_first(row)
-        self._validate_second(row)
-        self._validate_pair(row)
-        self._seen.add((row[self._sample_col], row[self._first_col]))
-        self.modified.append(row)
-
-    def _validate_sample(self, row):
-        """Assert that the sample name exists and convert spaces to underscores."""
-        if len(row[self._sample_col]) <= 0:
-            raise AssertionError("Sample input is required.")
-        # Sanitize samples slightly.
-        row[self._sample_col] = row[self._sample_col].replace(" ", "_")
-
-    def _validate_first(self, row):
-        """Assert that the first FASTQ entry is non-empty and has the right format."""
-        if len(row[self._first_col]) <= 0:
-            raise AssertionError("At least the first FASTQ file is required.")
-        self._validate_fastq_format(row[self._first_col])
-
-    def _validate_second(self, row):
-        """Assert that the second FASTQ entry has the right format if it exists."""
-        if len(row[self._second_col]) > 0:
-            self._validate_fastq_format(row[self._second_col])
-
-    def _validate_pair(self, row):
-        """Assert that read pairs have the same file extension. Report pair status."""
-        if row[self._first_col] and row[self._second_col]:
-            row[self._single_col] = False
-            first_col_suffix = Path(row[self._first_col]).suffixes[-2:]
-            second_col_suffix = Path(row[self._second_col]).suffixes[-2:]
-            if first_col_suffix != second_col_suffix:
-                raise AssertionError("FASTQ pairs must have the same file extensions.")
-        else:
-            row[self._single_col] = True
-
-    def _validate_fastq_format(self, filename):
-        """Assert that a given filename has one of the expected FASTQ extensions."""
-        if not any(filename.endswith(extension) for extension in self.VALID_FORMATS):
-            raise AssertionError(
-                f"The FASTQ file has an unrecognized extension: {filename}\n"
-                f"It should be one of: {', '.join(self.VALID_FORMATS)}"
-            )
-
-    def validate_unique_samples(self):
-        """
-        Assert that the combination of sample name and FASTQ filename is unique.
-
-        In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the
-        number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment.
-
-        """
-        if len(self._seen) != len(self.modified):
-            raise AssertionError("The pair of sample name and FASTQ must be unique.")
-        seen = Counter()
-        for row in self.modified:
-            sample = row[self._sample_col]
-            seen[sample] += 1
-            row[self._sample_col] = f"{sample}_T{seen[sample]}"
-
-
-def read_head(handle, num_lines=10):
-    """Read the specified number of lines from the current position in the file."""
-    lines = []
-    for idx, line in enumerate(handle):
-        if idx == num_lines:
-            break
-        lines.append(line)
-    return "".join(lines)
-
-
-def sniff_format(handle):
-    """
-    Detect the tabular format.
-
-    Args:
-        handle (text file): A handle to a `text file`_ object. The read position is
-        expected to be at the beginning (index 0).
-
-    Returns:
-        csv.Dialect: The detected tabular format.
-
-    .. _text file:
-        https://docs.python.org/3/glossary.html#term-text-file
-
-    """
-    peek = read_head(handle)
-    handle.seek(0)
-    sniffer = csv.Sniffer()
-    dialect = sniffer.sniff(peek)
-    return dialect
-
-
-def check_samplesheet(file_in, file_out):
-    """
-    Check that the tabular samplesheet has the structure expected by nf-core pipelines.
-
-    Validate the general shape of the table, expected columns, and each row. Also add
-    an additional column which records whether one or two FASTQ reads were found.
-
-    Args:
-        file_in (pathlib.Path): The given tabular samplesheet. The format can be either
-            CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``.
-        file_out (pathlib.Path): Where the validated and transformed samplesheet should
-            be created; always in CSV format.
-
-    Example:
-        This function checks that the samplesheet follows the following structure,
-        see also the `viral recon samplesheet`_::
-
-            sample,fastq_1,fastq_2
-            SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz
-            SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz
-            SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,
-
-    .. _viral recon samplesheet:
-        https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
-
-    """
-    required_columns = {"sample", "fastq_1", "fastq_2"}
-    # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`.
-    with file_in.open(newline="") as in_handle:
-        reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle))
-        # Validate the existence of the expected header columns.
-        if not required_columns.issubset(reader.fieldnames):
-            req_cols = ", ".join(required_columns)
-            logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.")
-            sys.exit(1)
-        # Validate each row.
-        checker = RowChecker()
-        for i, row in enumerate(reader):
-            try:
-                checker.validate_and_transform(row)
-            except AssertionError as error:
-                logger.critical(f"{str(error)} On line {i + 2}.")
-                sys.exit(1)
-        checker.validate_unique_samples()
-    header = list(reader.fieldnames)
-    header.insert(1, "single_end")
-    # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`.
-    with file_out.open(mode="w", newline="") as out_handle:
-        writer = csv.DictWriter(out_handle, header, delimiter=",")
-        writer.writeheader()
-        for row in checker.modified:
-            writer.writerow(row)
-
-
-def parse_args(argv=None):
-    """Define and immediately parse command line arguments."""
-    parser = argparse.ArgumentParser(
-        description="Validate and transform a tabular samplesheet.",
-        epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv",
-    )
-    parser.add_argument(
-        "file_in",
-        metavar="FILE_IN",
-        type=Path,
-        help="Tabular input samplesheet in CSV or TSV format.",
-    )
-    parser.add_argument(
-        "file_out",
-        metavar="FILE_OUT",
-        type=Path,
-        help="Transformed output samplesheet in CSV format.",
-    )
-    parser.add_argument(
-        "-l",
-        "--log-level",
-        help="The desired log level (default WARNING).",
-        choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"),
-        default="WARNING",
-    )
-    return parser.parse_args(argv)
-
-
-def main(argv=None):
-    """Coordinate argument parsing and program execution."""
-    args = parse_args(argv)
-    logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s")
-    if not args.file_in.is_file():
-        logger.error(f"The given input file {args.file_in} was not found!")
-        sys.exit(2)
-    args.file_out.parent.mkdir(parents=True, exist_ok=True)
-    check_samplesheet(args.file_in, args.file_out)
-
-
-if __name__ == "__main__":
-    sys.exit(main())
diff --git a/conf/modules.config b/conf/modules.config
index d91c6aba..e3ea8fa6 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -18,14 +18,6 @@ process {
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
-    withName: SAMPLESHEET_CHECK {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
     withName: FASTQC {
         ext.args = '--quiet'
     }
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
deleted file mode 100755
index e248e4c3..00000000
--- a/lib/NfcoreTemplate.groovy
+++ /dev/null
@@ -1,356 +0,0 @@
-//
-// This file holds several functions used within the nf-core pipeline template.
-//
-
-import org.yaml.snakeyaml.Yaml
-import groovy.json.JsonOutput
-import nextflow.extension.FilesEx
-
-class NfcoreTemplate {
-
-    //
-    // Check AWS Batch related parameters have been specified correctly
-    //
-    public static void awsBatch(workflow, params) {
-        if (workflow.profile.contains('awsbatch')) {
-            // Check params.awsqueue and params.awsregion have been set if running on AWSBatch
-            assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!"
-            // Check outdir paths to be S3 buckets if running on AWSBatch
-            assert params.outdir.startsWith('s3:')       : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!"
-        }
-    }
-
-    //
-    //  Warn if a -profile or Nextflow config has not been provided to run the pipeline
-    //
-    public static void checkConfigProvided(workflow, log) {
-        if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
-            log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
-                    "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
-                    "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
-                    "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
-                    "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
-                    "Please refer to the quick start section and usage docs for the pipeline.\n "
-        }
-    }
-
-    //
-    // Generate version string
-    //
-    public static String version(workflow) {
-        String version_string = ""
-
-        if (workflow.manifest.version) {
-            def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
-            version_string += "${prefix_v}${workflow.manifest.version}"
-        }
-
-        if (workflow.commitId) {
-            def git_shortsha = workflow.commitId.substring(0, 7)
-            version_string += "-g${git_shortsha}"
-        }
-
-        return version_string
-    }
-
-    //
-    // Construct and send completion email
-    //
-    public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) {
-
-        // Set up the e-mail variables
-        def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
-        if (!workflow.success) {
-            subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
-        }
-
-        def summary = [:]
-        for (group in summary_params.keySet()) {
-            summary << summary_params[group]
-        }
-
-        def misc_fields = [:]
-        misc_fields['Date Started']              = workflow.start
-        misc_fields['Date Completed']            = workflow.complete
-        misc_fields['Pipeline script file path'] = workflow.scriptFile
-        misc_fields['Pipeline script hash ID']   = workflow.scriptId
-        if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
-        if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
-        if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
-        misc_fields['Nextflow Version']           = workflow.nextflow.version
-        misc_fields['Nextflow Build']             = workflow.nextflow.build
-        misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
-
-        def email_fields = [:]
-        email_fields['version']      = NfcoreTemplate.version(workflow)
-        email_fields['runName']      = workflow.runName
-        email_fields['success']      = workflow.success
-        email_fields['dateComplete'] = workflow.complete
-        email_fields['duration']     = workflow.duration
-        email_fields['exitStatus']   = workflow.exitStatus
-        email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
-        email_fields['errorReport']  = (workflow.errorReport ?: 'None')
-        email_fields['commandLine']  = workflow.commandLine
-        email_fields['projectDir']   = workflow.projectDir
-        email_fields['summary']      = summary << misc_fields
-
-        // On success try attach the multiqc report
-        def mqc_report = null
-        try {
-            if (workflow.success) {
-                mqc_report = multiqc_report.getVal()
-                if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
-                    if (mqc_report.size() > 1) {
-                        log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
-                    }
-                    mqc_report = mqc_report[0]
-                }
-            }
-        } catch (all) {
-            if (multiqc_report) {
-                log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
-            }
-        }
-
-        // Check if we are only sending emails on failure
-        def email_address = params.email
-        if (!params.email && params.email_on_fail && !workflow.success) {
-            email_address = params.email_on_fail
-        }
-
-        // Render the TXT template
-        def engine       = new groovy.text.GStringTemplateEngine()
-        def tf           = new File("$projectDir/assets/email_template.txt")
-        def txt_template = engine.createTemplate(tf).make(email_fields)
-        def email_txt    = txt_template.toString()
-
-        // Render the HTML template
-        def hf            = new File("$projectDir/assets/email_template.html")
-        def html_template = engine.createTemplate(hf).make(email_fields)
-        def email_html    = html_template.toString()
-
-        // Render the sendmail template
-        def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
-        def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
-        def sf                     = new File("$projectDir/assets/sendmail_template.txt")
-        def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
-        def sendmail_html          = sendmail_template.toString()
-
-        // Send the HTML e-mail
-        Map colors = logColours(params.monochrome_logs)
-        if (email_address) {
-            try {
-                if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
-                // Try to send HTML e-mail using sendmail
-                def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
-                sendmail_tf.withWriter { w -> w << sendmail_html }
-                [ 'sendmail', '-t' ].execute() << sendmail_html
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
-            } catch (all) {
-                // Catch failures and try with plaintext
-                def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
-                if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
-                    mail_cmd += [ '-A', mqc_report ]
-                }
-                mail_cmd.execute() << email_html
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
-            }
-        }
-
-        // Write summary e-mail HTML to a file
-        def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
-        output_hf.withWriter { w -> w << email_html }
-        FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html");
-        output_hf.delete()
-
-        // Write summary e-mail TXT to a file
-        def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
-        output_tf.withWriter { w -> w << email_txt }
-        FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt");
-        output_tf.delete()
-    }
-
-    //
-    // Construct and send a notification to a web server as JSON
-    // e.g. Microsoft Teams and Slack
-    //
-    public static void IM_notification(workflow, params, summary_params, projectDir, log) {
-        def hook_url = params.hook_url
-
-        def summary = [:]
-        for (group in summary_params.keySet()) {
-            summary << summary_params[group]
-        }
-
-        def misc_fields = [:]
-        misc_fields['start']                                = workflow.start
-        misc_fields['complete']                             = workflow.complete
-        misc_fields['scriptfile']                           = workflow.scriptFile
-        misc_fields['scriptid']                             = workflow.scriptId
-        if (workflow.repository) misc_fields['repository']  = workflow.repository
-        if (workflow.commitId)   misc_fields['commitid']    = workflow.commitId
-        if (workflow.revision)   misc_fields['revision']    = workflow.revision
-        misc_fields['nxf_version']                          = workflow.nextflow.version
-        misc_fields['nxf_build']                            = workflow.nextflow.build
-        misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
-
-        def msg_fields = [:]
-        msg_fields['version']      = NfcoreTemplate.version(workflow)
-        msg_fields['runName']      = workflow.runName
-        msg_fields['success']      = workflow.success
-        msg_fields['dateComplete'] = workflow.complete
-        msg_fields['duration']     = workflow.duration
-        msg_fields['exitStatus']   = workflow.exitStatus
-        msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
-        msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
-        msg_fields['commandLine']  = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "")
-        msg_fields['projectDir']   = workflow.projectDir
-        msg_fields['summary']      = summary << misc_fields
-
-        // Render the JSON template
-        def engine       = new groovy.text.GStringTemplateEngine()
-        // Different JSON depending on the service provider
-        // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format
-        def json_path     = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json"
-        def hf            = new File("$projectDir/assets/${json_path}")
-        def json_template = engine.createTemplate(hf).make(msg_fields)
-        def json_message  = json_template.toString()
-
-        // POST
-        def post = new URL(hook_url).openConnection();
-        post.setRequestMethod("POST")
-        post.setDoOutput(true)
-        post.setRequestProperty("Content-Type", "application/json")
-        post.getOutputStream().write(json_message.getBytes("UTF-8"));
-        def postRC = post.getResponseCode();
-        if (! postRC.equals(200)) {
-            log.warn(post.getErrorStream().getText());
-        }
-    }
-
-    //
-    // Dump pipeline parameters in a json file
-    //
-    public static void dump_parameters(workflow, params) {
-        def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
-        def filename   = "params_${timestamp}.json"
-        def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
-        def jsonStr    = JsonOutput.toJson(params)
-        temp_pf.text   = JsonOutput.prettyPrint(jsonStr)
-
-        FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json")
-        temp_pf.delete()
-    }
-
-    //
-    // Print pipeline summary on completion
-    //
-    public static void summary(workflow, params, log) {
-        Map colors = logColours(params.monochrome_logs)
-        if (workflow.success) {
-            if (workflow.stats.ignoredCount == 0) {
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
-            } else {
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
-            }
-        } else {
-            log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
-        }
-    }
-
-    //
-    // ANSII Colours used for terminal logging
-    //
-    public static Map logColours(Boolean monochrome_logs) {
-        Map colorcodes = [:]
-
-        // Reset / Meta
-        colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
-        colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
-        colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
-        colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
-        colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
-        colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
-        colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
-
-        // Regular Colors
-        colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
-        colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
-        colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
-        colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
-        colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
-        colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
-        colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
-        colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
-
-        // Bold
-        colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
-        colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
-        colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
-        colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
-        colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
-        colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
-        colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
-        colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
-
-        // Underline
-        colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
-        colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
-        colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
-        colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
-        colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
-        colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
-        colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
-        colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
-
-        // High Intensity
-        colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
-        colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
-        colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
-        colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
-        colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
-        colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
-        colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
-        colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
-
-        // Bold High Intensity
-        colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
-        colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
-        colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
-        colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
-        colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
-        colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
-        colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
-        colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
-
-        return colorcodes
-    }
-
-    //
-    // Does what is says on the tin
-    //
-    public static String dashedLine(monochrome_logs) {
-        Map colors = logColours(monochrome_logs)
-        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
-    }
-
-    //
-    // nf-core logo
-    //
-    public static String logo(workflow, monochrome_logs) {
-        Map colors = logColours(monochrome_logs)
-        String workflow_version = NfcoreTemplate.version(workflow)
-        String.format(
-            """\n
-            ${dashedLine(monochrome_logs)}
-                                                    ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
-            ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
-            ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
-            ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
-                                                    ${colors.green}`._,._,\'${colors.reset}
-            ${colors.purple}  ${workflow.manifest.name} ${workflow_version}${colors.reset}
-            ${dashedLine(monochrome_logs)}
-            """.stripIndent()
-        )
-    }
-}
diff --git a/lib/Utils.groovy b/lib/Utils.groovy
deleted file mode 100644
index 8d030f4e..00000000
--- a/lib/Utils.groovy
+++ /dev/null
@@ -1,47 +0,0 @@
-//
-// This file holds several Groovy functions that could be useful for any Nextflow pipeline
-//
-
-import org.yaml.snakeyaml.Yaml
-
-class Utils {
-
-    //
-    // When running with -profile conda, warn if channels have not been set-up appropriately
-    //
-    public static void checkCondaChannels(log) {
-        Yaml parser = new Yaml()
-        def channels = []
-        try {
-            def config = parser.load("conda config --show channels".execute().text)
-            channels = config.channels
-        } catch(NullPointerException | IOException e) {
-            log.warn "Could not verify conda channel configuration."
-            return
-        }
-
-        // Check that all channels are present
-        // This channel list is ordered by required channel priority.
-        def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults']
-        def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
-
-        // Check that they are in the right order
-        def channel_priority_violation = false
-        def n = required_channels_in_order.size()
-        for (int i = 0; i < n - 1; i++) {
-            channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1]))
-        }
-
-        if (channels_missing | channel_priority_violation) {
-            log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
-                "  There is a problem with your Conda configuration!\n\n" +
-                "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
-                "  Please refer to https://bioconda.github.io/\n" +
-                "  The observed channel order is \n" +
-                "  ${channels}\n" +
-                "  but the following channel order is required:\n" +
-                "  ${required_channels_in_order}\n" +
-                "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
-        }
-    }
-}
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
deleted file mode 100755
index 22deebd8..00000000
--- a/lib/WorkflowMain.groovy
+++ /dev/null
@@ -1,77 +0,0 @@
-//
-// This file holds several functions specific to the main.nf workflow in the nf-core/smrnaseq pipeline
-//
-
-import nextflow.Nextflow
-
-class WorkflowMain {
-
-    //
-    // Citation string for pipeline
-    //
-    public static String citation(workflow) {
-        return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
-            // TODO nf-core: Add Zenodo DOI for pipeline after first release
-            //"* The pipeline\n" +
-            //"  https://doi.org/10.5281/zenodo.XXXXXXX\n\n" +
-            "* The nf-core framework\n" +
-            "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
-            "* Software dependencies\n" +
-            "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
-    }
-
-
-    //
-    // Validate parameters and print summary to screen
-    //
-    public static void initialise(workflow, params, log, args) {
-
-        // Print workflow version and exit on --version
-        if (params.version) {
-            String workflow_version = NfcoreTemplate.version(workflow)
-            log.info "${workflow.manifest.name} ${workflow_version}"
-            System.exit(0)
-        }
-
-        // Check that a -profile or Nextflow config has been provided to run the pipeline
-        NfcoreTemplate.checkConfigProvided(workflow, log)
-        // Check that the profile doesn't contain spaces and doesn't end with a trailing comma
-        checkProfile(workflow.profile, args, log)
-
-        // Check that conda channels are set-up correctly
-        if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-            Utils.checkCondaChannels(log)
-        }
-
-        // Check AWS batch settings
-        NfcoreTemplate.awsBatch(workflow, params)
-
-        // Check input has been provided
-        if (!params.input) {
-            Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'")
-        }
-    }
-    //
-    // Get attribute from genome config file e.g. fasta
-    //
-    public static Object getGenomeAttribute(params, attribute) {
-        if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) {
-            if (params.genomes[ params.genome ].containsKey(attribute)) {
-                return params.genomes[ params.genome ][ attribute ]
-            }
-        }
-        return null
-    }
-
-    //
-    // Exit pipeline if --profile contains spaces
-    //
-    private static void checkProfile(profile, args, log) {
-        if (profile.endsWith(',')) {
-            Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`."
-        }
-        if (args[0]) {
-            log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n      Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`."
-        }
-    }
-}
diff --git a/lib/WorkflowSmrnaseq.groovy b/lib/WorkflowSmrnaseq.groovy
deleted file mode 100755
index 148049e2..00000000
--- a/lib/WorkflowSmrnaseq.groovy
+++ /dev/null
@@ -1,122 +0,0 @@
-//
-// This file holds several functions specific to the workflow/smrnaseq.nf in the nf-core/smrnaseq pipeline
-//
-
-import nextflow.Nextflow
-import groovy.text.SimpleTemplateEngine
-
-class WorkflowSmrnaseq {
-
-    //
-    // Check and validate parameters
-    //
-    public static void initialise(params, log) {
-
-        genomeExistsError(params, log)
-
-
-        if (!params.fasta) {
-            Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
-        }
-    }
-
-    //
-    // Get workflow summary for MultiQC
-    //
-    public static String paramsSummaryMultiqc(workflow, summary) {
-        String summary_section = ''
-        for (group in summary.keySet()) {
-            def group_params = summary.get(group)  // This gets the parameters of that particular group
-            if (group_params) {
-                summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
-                summary_section += "    <dl class=\"dl-horizontal\">\n"
-                for (param in group_params.keySet()) {
-                    summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
-                }
-                summary_section += "    </dl>\n"
-            }
-        }
-
-        String yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n"
-        yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
-        yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
-        yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
-        yaml_file_text        += "plot_type: 'html'\n"
-        yaml_file_text        += "data: |\n"
-        yaml_file_text        += "${summary_section}"
-        return yaml_file_text
-    }
-
-    //
-    // Generate methods description for MultiQC
-    //
-
-    public static String toolCitationText(params) {
-
-        // TODO nf-core: Optionally add in-text citation tools to this list.
-        // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "",
-        // Uncomment function in methodsDescriptionText to render in MultiQC report
-        def citation_text = [
-                "Tools used in the workflow included:",
-                "FastQC (Andrews 2010),",
-                "MultiQC (Ewels et al. 2016)",
-                "."
-            ].join(' ').trim()
-
-        return citation_text
-    }
-
-    public static String toolBibliographyText(params) {
-
-        // TODO Optionally add bibliographic entries to this list.
-        // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "<li>Author (2023) Pub name, Journal, DOI</li>" : "",
-        // Uncomment function in methodsDescriptionText to render in MultiQC report
-        def reference_text = [
-                "<li>Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).</li>",
-                "<li>Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354</li>"
-            ].join(' ').trim()
-
-        return reference_text
-    }
-
-    public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) {
-        // Convert  to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file
-        def meta = [:]
-        meta.workflow = run_workflow.toMap()
-        meta["manifest_map"] = run_workflow.manifest.toMap()
-
-        // Pipeline DOI
-        meta["doi_text"] = meta.manifest_map.doi ? "(doi: <a href=\'https://doi.org/${meta.manifest_map.doi}\'>${meta.manifest_map.doi}</a>)" : ""
-        meta["nodoi_text"] = meta.manifest_map.doi ? "": "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
-
-        // Tool references
-        meta["tool_citations"] = ""
-        meta["tool_bibliography"] = ""
-
-        // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!
-        //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".")
-        //meta["tool_bibliography"] = toolBibliographyText(params)
-
-
-        def methods_text = mqc_methods_yaml.text
-
-        def engine =  new SimpleTemplateEngine()
-        def description_html = engine.createTemplate(methods_text).make(meta)
-
-        return description_html
-    }
-
-    //
-    // Exit pipeline if incorrect --genome key provided
-    //
-    private static void genomeExistsError(params, log) {
-        if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
-            def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
-                "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
-                "  Currently, the available genome keys are:\n" +
-                "  ${params.genomes.keySet().join(", ")}\n" +
-                "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
-            Nextflow.error(error_string)
-        }
-    }
-}
diff --git a/main.nf b/main.nf
index 8be7e198..784b2be3 100644
--- a/main.nf
+++ b/main.nf
@@ -13,66 +13,96 @@ nextflow.enable.dsl = 2
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    GENOME PARAMETER VALUES
+    IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-// TODO nf-core: Remove this line if you don't need a FASTA file
-//   This is an example of how to use getGenomeAttribute() to fetch parameters
-//   from igenomes.config using `--genome`
-params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
+include { SMRNASEQ  } from './workflows/smrnaseq'
+include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_smrnaseq_pipeline'
+include { PIPELINE_COMPLETION     } from './subworkflows/local/utils_nfcore_smrnaseq_pipeline'
+
+include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_smrnaseq_pipeline'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    VALIDATE & PRINT PARAMETER SUMMARY
+    GENOME PARAMETER VALUES
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { validateParameters; paramsHelp } from 'plugin/nf-validation'
-
-// Print help message if needed
-if (params.help) {
-    def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs)
-    def citation = '\n' + WorkflowMain.citation(workflow) + '\n'
-    def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker"
-    log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs)
-    System.exit(0)
-}
-
-// Validate input parameters
-if (params.validate_params) {
-    validateParameters()
-}
-
-WorkflowMain.initialise(workflow, params, log, args)
+// TODO nf-core: Remove this line if you don't need a FASTA file
+//   This is an example of how to use getGenomeAttribute() to fetch parameters
+//   from igenomes.config using `--genome`
+params.fasta = getGenomeAttribute('fasta')
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    NAMED WORKFLOW FOR PIPELINE
+    NAMED WORKFLOWS FOR PIPELINE
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { SMRNASEQ } from './workflows/smrnaseq'
-
 //
-// WORKFLOW: Run main nf-core/smrnaseq analysis pipeline
+// WORKFLOW: Run main analysis pipeline depending on type of input
 //
 workflow NFCORE_SMRNASEQ {
-    SMRNASEQ ()
-}
 
+    take:
+    samplesheet // channel: samplesheet read in from --input
+
+    main:
+
+    //
+    // WORKFLOW: Run pipeline
+    //
+    SMRNASEQ (
+        samplesheet
+    )
+
+    emit:
+    multiqc_report = SMRNASEQ.out.multiqc_report // channel: /path/to/multiqc_report.html
+
+}
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    RUN ALL WORKFLOWS
+    RUN MAIN WORKFLOW
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-//
-// WORKFLOW: Execute a single named workflow for the pipeline
-// See: https://github.com/nf-core/rnaseq/issues/619
-//
 workflow {
-    NFCORE_SMRNASEQ ()
+
+    main:
+
+    //
+    // SUBWORKFLOW: Run initialisation tasks
+    //
+    PIPELINE_INITIALISATION (
+        params.version,
+        params.help,
+        params.validate_params,
+        params.monochrome_logs,
+        args,
+        params.outdir,
+        params.input
+    )
+
+    //
+    // WORKFLOW: Run main workflow
+    //
+    NFCORE_SMRNASEQ (
+        PIPELINE_INITIALISATION.out.samplesheet
+    )
+
+    //
+    // SUBWORKFLOW: Run completion tasks
+    //
+    PIPELINE_COMPLETION (
+        params.email,
+        params.email_on_fail,
+        params.plaintext_email,
+        params.outdir,
+        params.monochrome_logs,
+        params.hook_url,
+        NFCORE_SMRNASEQ.out.multiqc_report
+    )
 }
 
 /*
diff --git a/modules.json b/modules.json
index f3d71e3b..812aef49 100644
--- a/modules.json
+++ b/modules.json
@@ -5,22 +5,36 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
-                    "custom/dumpsoftwareversions": {
-                        "branch": "master",
-                        "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
-                        "installed_by": ["modules"]
-                    },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9",
+                        "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93",
+                        "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290",
                         "installed_by": ["modules"]
                     }
                 }
+            },
+            "subworkflows": {
+                "nf-core": {
+                    "utils_nextflow_pipeline": {
+                        "branch": "master",
+                        "git_sha": "cd08c91373cd00a73255081340e4914485846ba1",
+                        "installed_by": ["subworkflows"]
+                    },
+                    "utils_nfcore_pipeline": {
+                        "branch": "master",
+                        "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8",
+                        "installed_by": ["subworkflows"]
+                    },
+                    "utils_nfvalidation_plugin": {
+                        "branch": "master",
+                        "git_sha": "cd08c91373cd00a73255081340e4914485846ba1",
+                        "installed_by": ["subworkflows"]
+                    }
+                }
             }
         }
     }
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
deleted file mode 100644
index 351d2322..00000000
--- a/modules/local/samplesheet_check.nf
+++ /dev/null
@@ -1,31 +0,0 @@
-process SAMPLESHEET_CHECK {
-    tag "$samplesheet"
-    label 'process_single'
-
-    conda "conda-forge::python=3.8.3"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/python:3.8.3' :
-        'biocontainers/python:3.8.3' }"
-
-    input:
-    path samplesheet
-
-    output:
-    path '*.csv'       , emit: csv
-    path "versions.yml", emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script: // This script is bundled with the pipeline, in nf-core/smrnaseq/bin/
-    """
-    check_samplesheet.py \\
-        $samplesheet \\
-        samplesheet.valid.csv
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        python: \$(python --version | sed 's/Python //g')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
deleted file mode 100644
index 9b3272bc..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-name: custom_dumpsoftwareversions
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::multiqc=1.19
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
deleted file mode 100644
index f2187611..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ /dev/null
@@ -1,24 +0,0 @@
-process CUSTOM_DUMPSOFTWAREVERSIONS {
-    label 'process_single'
-
-    // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.19--pyhdfd78af_0' }"
-
-    input:
-    path versions
-
-    output:
-    path "software_versions.yml"    , emit: yml
-    path "software_versions_mqc.yml", emit: mqc_yml
-    path "versions.yml"             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    template 'dumpsoftwareversions.py'
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
deleted file mode 100644
index 5f15a5fd..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ /dev/null
@@ -1,37 +0,0 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
-name: custom_dumpsoftwareversions
-description: Custom module used to dump software versions within the nf-core pipeline template
-keywords:
-  - custom
-  - dump
-  - version
-tools:
-  - custom:
-      description: Custom module used to dump software versions within the nf-core pipeline template
-      homepage: https://github.com/nf-core/tools
-      documentation: https://github.com/nf-core/tools
-      licence: ["MIT"]
-input:
-  - versions:
-      type: file
-      description: YML file containing software versions
-      pattern: "*.yml"
-output:
-  - yml:
-      type: file
-      description: Standard YML file containing software versions
-      pattern: "software_versions.yml"
-  - mqc_yml:
-      type: file
-      description: MultiQC custom content YML file containing software versions
-      pattern: "software_versions_mqc.yml"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@drpatelh"
-  - "@grst"
-maintainers:
-  - "@drpatelh"
-  - "@grst"
diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
deleted file mode 100755
index e55b8d43..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
+++ /dev/null
@@ -1,102 +0,0 @@
-#!/usr/bin/env python
-
-
-"""Provide functions to merge multiple versions.yml files."""
-
-
-import platform
-from textwrap import dedent
-
-import yaml
-
-
-def _make_versions_html(versions):
-    """Generate a tabular HTML output of all versions for MultiQC."""
-    html = [
-        dedent(
-            """\\
-            <style>
-            #nf-core-versions tbody:nth-child(even) {
-                background-color: #f2f2f2;
-            }
-            </style>
-            <table class="table" style="width:100%" id="nf-core-versions">
-                <thead>
-                    <tr>
-                        <th> Process Name </th>
-                        <th> Software </th>
-                        <th> Version  </th>
-                    </tr>
-                </thead>
-            """
-        )
-    ]
-    for process, tmp_versions in sorted(versions.items()):
-        html.append("<tbody>")
-        for i, (tool, version) in enumerate(sorted(tmp_versions.items())):
-            html.append(
-                dedent(
-                    f"""\\
-                    <tr>
-                        <td><samp>{process if (i == 0) else ''}</samp></td>
-                        <td><samp>{tool}</samp></td>
-                        <td><samp>{version}</samp></td>
-                    </tr>
-                    """
-                )
-            )
-        html.append("</tbody>")
-    html.append("</table>")
-    return "\\n".join(html)
-
-
-def main():
-    """Load all version files and generate merged output."""
-    versions_this_module = {}
-    versions_this_module["${task.process}"] = {
-        "python": platform.python_version(),
-        "yaml": yaml.__version__,
-    }
-
-    with open("$versions") as f:
-        versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
-
-    # aggregate versions by the module name (derived from fully-qualified process name)
-    versions_by_module = {}
-    for process, process_versions in versions_by_process.items():
-        module = process.split(":")[-1]
-        try:
-            if versions_by_module[module] != process_versions:
-                raise AssertionError(
-                    "We assume that software versions are the same between all modules. "
-                    "If you see this error-message it means you discovered an edge-case "
-                    "and should open an issue in nf-core/tools. "
-                )
-        except KeyError:
-            versions_by_module[module] = process_versions
-
-    versions_by_module["Workflow"] = {
-        "Nextflow": "$workflow.nextflow.version",
-        "$workflow.manifest.name": "$workflow.manifest.version",
-    }
-
-    versions_mqc = {
-        "id": "software_versions",
-        "section_name": "${workflow.manifest.name} Software Versions",
-        "section_href": "https://github.com/${workflow.manifest.name}",
-        "plot_type": "html",
-        "description": "are collected at run time from the software output.",
-        "data": _make_versions_html(versions_by_module),
-    }
-
-    with open("software_versions.yml", "w") as f:
-        yaml.dump(versions_by_module, f, default_flow_style=False)
-    with open("software_versions_mqc.yml", "w") as f:
-        yaml.dump(versions_mqc, f, default_flow_style=False)
-
-    with open("versions.yml", "w") as f:
-        yaml.dump(versions_this_module, f, default_flow_style=False)
-
-
-if __name__ == "__main__":
-    main()
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
deleted file mode 100644
index b1e1630b..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
+++ /dev/null
@@ -1,43 +0,0 @@
-nextflow_process {
-
-    name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS"
-    script "../main.nf"
-    process "CUSTOM_DUMPSOFTWAREVERSIONS"
-    tag "modules"
-    tag "modules_nfcore"
-    tag "custom"
-    tag "dumpsoftwareversions"
-    tag "custom/dumpsoftwareversions"
-
-    test("Should run without failures") {
-        when {
-            process {
-                """
-                def tool1_version = '''
-                TOOL1:
-                    tool1: 0.11.9
-                '''.stripIndent()
-
-                def tool2_version = '''
-                TOOL2:
-                    tool2: 1.9
-                '''.stripIndent()
-
-                input[0] = Channel.of(tool1_version, tool2_version).collectFile()
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.versions,
-                    file(process.out.mqc_yml[0]).readLines()[0..10],
-                    file(process.out.yml[0]).readLines()[0..7]
-                    ).match()
-                }
-            )
-        }
-    }
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
deleted file mode 100644
index 5f59a936..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
+++ /dev/null
@@ -1,33 +0,0 @@
-{
-    "Should run without failures": {
-        "content": [
-            [
-                "versions.yml:md5,76d454d92244589d32455833f7c1ba6d"
-            ],
-            [
-                "data: \"<style>\\n#nf-core-versions tbody:nth-child(even) {\\n    background-color: #f2f2f2;\\n\\",
-                "  }\\n</style>\\n<table class=\\\"table\\\" style=\\\"width:100%\\\" id=\\\"nf-core-versions\\\"\\",
-                "  >\\n    <thead>\\n        <tr>\\n            <th> Process Name </th>\\n            <th>\\",
-                "  \\ Software </th>\\n            <th> Version  </th>\\n        </tr>\\n    </thead>\\n\\",
-                "  \\n<tbody>\\n<tr>\\n    <td><samp>CUSTOM_DUMPSOFTWAREVERSIONS</samp></td>\\n    <td><samp>python</samp></td>\\n\\",
-                "  \\    <td><samp>3.11.7</samp></td>\\n</tr>\\n\\n<tr>\\n    <td><samp></samp></td>\\n \\",
-                "  \\   <td><samp>yaml</samp></td>\\n    <td><samp>5.4.1</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
-                "  <tbody>\\n<tr>\\n    <td><samp>TOOL1</samp></td>\\n    <td><samp>tool1</samp></td>\\n\\",
-                "  \\    <td><samp>0.11.9</samp></td>\\n</tr>\\n\\n</tbody>\\n<tbody>\\n<tr>\\n    <td><samp>TOOL2</samp></td>\\n\\",
-                "  \\    <td><samp>tool2</samp></td>\\n    <td><samp>1.9</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
-                "  <tbody>\\n<tr>\\n    <td><samp>Workflow</samp></td>\\n    <td><samp>Nextflow</samp></td>\\n\\"
-            ],
-            [
-                "CUSTOM_DUMPSOFTWAREVERSIONS:",
-                "  python: 3.11.7",
-                "  yaml: 5.4.1",
-                "TOOL1:",
-                "  tool1: 0.11.9",
-                "TOOL2:",
-                "  tool2: '1.9'",
-                "Workflow:"
-            ]
-        ],
-        "timestamp": "2024-01-09T23:01:18.710682"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
deleted file mode 100644
index 405aa24a..00000000
--- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-custom/dumpsoftwareversions:
-  - modules/nf-core/custom/dumpsoftwareversions/**
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
index 1f21c664..70edae4d 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -33,7 +33,7 @@ nextflow_process {
             { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
             { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_single") }
             )
         }
     }
@@ -63,7 +63,7 @@ nextflow_process {
             { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
             { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_paired") }
             )
         }
     }
@@ -89,7 +89,7 @@ nextflow_process {
             { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
             { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") }
             )
         }
     }
@@ -115,7 +115,7 @@ nextflow_process {
             { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
             { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_bam") }
             )
         }
     }
@@ -153,7 +153,7 @@ nextflow_process {
             { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
             { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_multiple") }
             )
         }
     }
@@ -179,7 +179,7 @@ nextflow_process {
             { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
             { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
 
-            { assert snapshot(process.out.versions).match("versions") }
+            { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") }
             )
         }
     }
@@ -204,7 +204,7 @@ nextflow_process {
             { assert process.success },
             { assert snapshot(process.out.html.collect { file(it[1]).getName() } +
                                 process.out.zip.collect { file(it[1]).getName() } +
-                                process.out.versions ).match() }
+                                process.out.versions ).match("fastqc_stub") }
             )
         }
     }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
index 5d624bb8..86f7c311 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test.snap
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -1,5 +1,17 @@
 {
-    "sarscov2 single-end [fastq] - stub": {
+    "fastqc_versions_interleaved": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:40:07.293713"
+    },
+    "fastqc_stub": {
         "content": [
             [
                 "test.html",
@@ -7,14 +19,70 @@
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
-        "timestamp": "2024-01-17T18:40:57.254299"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:31:01.425198"
+    },
+    "fastqc_versions_multiple": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:40:55.797907"
+    },
+    "fastqc_versions_bam": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:40:26.795862"
+    },
+    "fastqc_versions_single": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:39:27.043675"
+    },
+    "fastqc_versions_paired": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:39:47.584191"
     },
-    "versions": {
+    "fastqc_versions_custom_prefix": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
-        "timestamp": "2024-01-17T18:36:50.033627"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-31T17:41:14.576531"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index 7625b752..2212096a 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::multiqc=1.19
+  - bioconda::multiqc=1.20
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 1b9f7c43..354f4430 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.19--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.20--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
index d0438eda..f1c4242e 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -3,6 +3,7 @@ nextflow_process {
     name "Test Process MULTIQC"
     script "../main.nf"
     process "MULTIQC"
+
     tag "modules"
     tag "modules_nfcore"
     tag "multiqc"
@@ -12,7 +13,7 @@ nextflow_process {
         when {
             process {
                 """
-                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
                 input[1] = []
                 input[2] = []
                 input[3] = []
@@ -25,7 +26,7 @@ nextflow_process {
                 { assert process.success },
                 { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
                 { assert process.out.data[0] ==~ ".*/multiqc_data" },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("multiqc_versions_single") }
             )
         }
 
@@ -36,7 +37,7 @@ nextflow_process {
         when {
             process {
                 """
-                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
                 input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
                 input[2] = []
                 input[3] = []
@@ -49,7 +50,7 @@ nextflow_process {
                 { assert process.success },
                 { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
                 { assert process.out.data[0] ==~ ".*/multiqc_data" },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("multiqc_versions_config") }
             )
         }
     }
@@ -61,7 +62,7 @@ nextflow_process {
         when {
             process {
                 """
-                input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)])
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
                 input[1] = []
                 input[2] = []
                 input[3] = []
@@ -75,7 +76,7 @@ nextflow_process {
                 { assert snapshot(process.out.report.collect { file(it).getName() } +
                                 process.out.data.collect { file(it).getName() } +
                                 process.out.plots.collect { file(it).getName() } +
-                                process.out.versions ).match() }
+                                process.out.versions ).match("multiqc_stub") }
             )
         }
 
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index d37e7304..c204b488 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -1,21 +1,41 @@
 {
-    "versions": {
+    "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d"
+                "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186"
             ]
         ],
-        "timestamp": "2024-01-09T23:02:49.911994"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-14T09:28:51.744211298"
     },
-    "sarscov2 single-end [fastqc] - stub": {
+    "multiqc_stub": {
         "content": [
             [
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d"
+                "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186"
             ]
         ],
-        "timestamp": "2024-01-09T23:03:14.524346"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-14T09:29:28.847433492"
+    },
+    "multiqc_versions_config": {
+        "content": [
+            [
+                "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-14T09:29:13.223621555"
     }
 }
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index 02c8d3e2..8aa09a78 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -16,9 +16,7 @@ params {
     genome                     = null
     igenomes_base              = 's3://ngi-igenomes/igenomes/'
     igenomes_ignore            = false
-    
-
-    // MultiQC options
+    fasta                      = null// MultiQC options
     multiqc_config             = null
     multiqc_title              = null
     multiqc_logo               = null
@@ -43,7 +41,6 @@ params {
     custom_config_base         = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
     config_profile_contact     = null
     config_profile_url         = null
-    
 
     // Max resource options
     // Defaults only, expecting to be overwritten
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 764f18b2..1a8835a1 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -16,6 +16,7 @@
                     "type": "string",
                     "format": "file-path",
                     "exists": true,
+                    "schema": "assets/schema_input.json",
                     "mimetype": "text/csv",
                     "pattern": "^\\S+\\.csv$",
                     "description": "Path to comma-separated file containing information about the samples in the experiment.",
diff --git a/pyproject.toml b/pyproject.toml
index 7d08e1c8..56110621 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -3,11 +3,13 @@
 [tool.ruff]
 line-length = 120
 target-version = "py38"
-select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"]
 cache-dir = "~/.cache/ruff"
 
-[tool.ruff.isort]
+[tool.ruff.lint]
+select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"]
+
+[tool.ruff.lint.isort]
 known-first-party = ["nf_core"]
 
-[tool.ruff.per-file-ignores]
+[tool.ruff.lint.per-file-ignores]
 "__init__.py" = ["E402", "F401"]
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
deleted file mode 100644
index 0aecf87f..00000000
--- a/subworkflows/local/input_check.nf
+++ /dev/null
@@ -1,44 +0,0 @@
-//
-// Check input samplesheet and get read channels
-//
-
-include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check'
-
-workflow INPUT_CHECK {
-    take:
-    samplesheet // file: /path/to/samplesheet.csv
-
-    main:
-    SAMPLESHEET_CHECK ( samplesheet )
-        .csv
-        .splitCsv ( header:true, sep:',' )
-        .map { create_fastq_channel(it) }
-        .set { reads }
-
-    emit:
-    reads                                     // channel: [ val(meta), [ reads ] ]
-    versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ]
-}
-
-// Function to get list of [ meta, [ fastq_1, fastq_2 ] ]
-def create_fastq_channel(LinkedHashMap row) {
-    // create meta map
-    def meta = [:]
-    meta.id         = row.sample
-    meta.single_end = row.single_end.toBoolean()
-
-    // add path(s) of the fastq file(s) to the meta map
-    def fastq_meta = []
-    if (!file(row.fastq_1).exists()) {
-        exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}"
-    }
-    if (meta.single_end) {
-        fastq_meta = [ meta, [ file(row.fastq_1) ] ]
-    } else {
-        if (!file(row.fastq_2).exists()) {
-            exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}"
-        }
-        fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ]
-    }
-    return fastq_meta
-}
diff --git a/subworkflows/local/utils_nfcore_smrnaseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_smrnaseq_pipeline/main.nf
new file mode 100644
index 00000000..dc90ad90
--- /dev/null
+++ b/subworkflows/local/utils_nfcore_smrnaseq_pipeline/main.nf
@@ -0,0 +1,247 @@
+//
+// Subworkflow with functionality specific to the nf-core/pipeline pipeline
+//
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin'
+include { paramsSummaryMap          } from 'plugin/nf-validation'
+include { fromSamplesheet           } from 'plugin/nf-validation'
+include { UTILS_NEXTFLOW_PIPELINE   } from '../../nf-core/utils_nextflow_pipeline'
+include { completionEmail           } from '../../nf-core/utils_nfcore_pipeline'
+include { completionSummary         } from '../../nf-core/utils_nfcore_pipeline'
+include { dashedLine                } from '../../nf-core/utils_nfcore_pipeline'
+include { nfCoreLogo                } from '../../nf-core/utils_nfcore_pipeline'
+include { imNotification            } from '../../nf-core/utils_nfcore_pipeline'
+include { UTILS_NFCORE_PIPELINE     } from '../../nf-core/utils_nfcore_pipeline'
+include { workflowCitation          } from '../../nf-core/utils_nfcore_pipeline'
+
+/*
+========================================================================================
+    SUBWORKFLOW TO INITIALISE PIPELINE
+========================================================================================
+*/
+
+workflow PIPELINE_INITIALISATION {
+
+    take:
+    version           // boolean: Display version and exit
+    help              // boolean: Display help text
+    validate_params   // boolean: Boolean whether to validate parameters against the schema at runtime
+    monochrome_logs   // boolean: Do not use coloured log outputs
+    nextflow_cli_args //   array: List of positional nextflow CLI args
+    outdir            //  string: The output directory where the results will be saved
+    input             //  string: Path to input samplesheet
+
+    main:
+
+    ch_versions = Channel.empty()
+
+    //
+    // Print version and exit if required and dump pipeline parameters to JSON file
+    //
+    UTILS_NEXTFLOW_PIPELINE (
+        version,
+        true,
+        outdir,
+        workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1
+    )
+
+    //
+    // Validate parameters and generate parameter summary to stdout
+    //
+    pre_help_text = nfCoreLogo(monochrome_logs)
+    post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs)
+    def String workflow_command = "nextflow run ${workflow.manifest.name} -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>"
+    UTILS_NFVALIDATION_PLUGIN (
+        help,
+        workflow_command,
+        pre_help_text,
+        post_help_text,
+        validate_params,
+        "nextflow_schema.json"
+    )
+
+    //
+    // Check config provided to the pipeline
+    //
+    UTILS_NFCORE_PIPELINE (
+        nextflow_cli_args
+    )
+    //
+    // Custom validation for pipeline parameters
+    //
+    validateInputParameters()
+
+    //
+    // Create channel from input file provided through params.input
+    //
+    Channel
+        .fromSamplesheet("input")
+        .map {
+            meta, fastq_1, fastq_2 ->
+                if (!fastq_2) {
+                    return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
+                } else {
+                    return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
+                }
+        }
+        .groupTuple()
+        .map {
+            validateInputSamplesheet(it)
+        }
+        .map {
+            meta, fastqs ->
+                return [ meta, fastqs.flatten() ]
+        }
+        .set { ch_samplesheet }
+
+    emit:
+    samplesheet = ch_samplesheet
+    versions    = ch_versions
+}
+
+/*
+========================================================================================
+    SUBWORKFLOW FOR PIPELINE COMPLETION
+========================================================================================
+*/
+
+workflow PIPELINE_COMPLETION {
+
+    take:
+    email           //  string: email address
+    email_on_fail   //  string: email address sent on pipeline failure
+    plaintext_email // boolean: Send plain-text email instead of HTML
+    outdir          //    path: Path to output directory where results will be published
+    monochrome_logs // boolean: Disable ANSI colour codes in log output
+    hook_url        //  string: hook URL for notifications
+    multiqc_report  //  string: Path to MultiQC report
+
+    main:
+
+    summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
+
+    //
+    // Completion email and summary
+    //
+    workflow.onComplete {
+        if (email || email_on_fail) {
+            completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList())
+        }
+
+        completionSummary(monochrome_logs)
+
+        if (hook_url) {
+            imNotification(summary_params, hook_url)
+        }
+    }
+}
+
+/*
+========================================================================================
+    FUNCTIONS
+========================================================================================
+*/
+//
+// Check and validate pipeline parameters
+//
+def validateInputParameters() {
+    genomeExistsError()
+}//
+// Validate channels from input samplesheet
+//
+def validateInputSamplesheet(input) {
+    def (metas, fastqs) = input[1..2]
+
+    // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end
+    def endedness_ok = metas.collect{ it.single_end }.unique().size == 1
+    if (!endedness_ok) {
+        error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}")
+    }
+
+    return [ metas[0], fastqs ]
+}
+//
+// Get attribute from genome config file e.g. fasta
+//
+def getGenomeAttribute(attribute) {
+    if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) {
+        if (params.genomes[ params.genome ].containsKey(attribute)) {
+            return params.genomes[ params.genome ][ attribute ]
+        }
+    }
+    return null
+}
+
+//
+// Exit pipeline if incorrect --genome key provided
+//
+def genomeExistsError() {
+    if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
+        def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
+            "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
+            "  Currently, the available genome keys are:\n" +
+            "  ${params.genomes.keySet().join(", ")}\n" +
+            "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+        error(error_string)
+    }
+}//
+// Generate methods description for MultiQC
+//
+def toolCitationText() {
+    // TODO nf-core: Optionally add in-text citation tools to this list.
+    // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "",
+    // Uncomment function in methodsDescriptionText to render in MultiQC report
+    def citation_text = [
+            "Tools used in the workflow included:",
+            "FastQC (Andrews 2010),",
+            "MultiQC (Ewels et al. 2016)",
+            "."
+        ].join(' ').trim()
+
+    return citation_text
+}
+
+def toolBibliographyText() {
+    // TODO nf-core: Optionally add bibliographic entries to this list.
+    // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "<li>Author (2023) Pub name, Journal, DOI</li>" : "",
+    // Uncomment function in methodsDescriptionText to render in MultiQC report
+    def reference_text = [
+            "<li>Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).</li>",
+            "<li>Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354</li>"
+        ].join(' ').trim()
+
+    return reference_text
+}
+
+def methodsDescriptionText(mqc_methods_yaml) {
+    // Convert  to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file
+    def meta = [:]
+    meta.workflow = workflow.toMap()
+    meta["manifest_map"] = workflow.manifest.toMap()
+
+    // Pipeline DOI
+    meta["doi_text"] = meta.manifest_map.doi ? "(doi: <a href=\'https://doi.org/${meta.manifest_map.doi}\'>${meta.manifest_map.doi}</a>)" : ""
+    meta["nodoi_text"] = meta.manifest_map.doi ? "": "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
+
+    // Tool references
+    meta["tool_citations"] = ""
+    meta["tool_bibliography"] = ""
+
+    // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!
+    // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".")
+    // meta["tool_bibliography"] = toolBibliographyText()
+
+
+    def methods_text = mqc_methods_yaml.text
+
+    def engine =  new groovy.text.SimpleTemplateEngine()
+    def description_html = engine.createTemplate(methods_text).make(meta)
+
+    return description_html.toString()
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
new file mode 100644
index 00000000..ac31f28f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
@@ -0,0 +1,126 @@
+//
+// Subworkflow with functionality that may be useful for any Nextflow pipeline
+//
+
+import org.yaml.snakeyaml.Yaml
+import groovy.json.JsonOutput
+import nextflow.extension.FilesEx
+
+/*
+========================================================================================
+    SUBWORKFLOW DEFINITION
+========================================================================================
+*/
+
+workflow UTILS_NEXTFLOW_PIPELINE {
+
+    take:
+    print_version        // boolean: print version
+    dump_parameters      // boolean: dump parameters
+    outdir               //    path: base directory used to publish pipeline results
+    check_conda_channels // boolean: check conda channels
+
+    main:
+
+    //
+    // Print workflow version and exit on --version
+    //
+    if (print_version) {
+        log.info "${workflow.manifest.name} ${getWorkflowVersion()}"
+        System.exit(0)
+    }
+
+    //
+    // Dump pipeline parameters to a JSON file
+    //
+    if (dump_parameters && outdir) {
+        dumpParametersToJSON(outdir)
+    }
+
+    //
+    // When running with Conda, warn if channels have not been set-up appropriately
+    //
+    if (check_conda_channels) {
+        checkCondaChannels()
+    }
+
+    emit:
+    dummy_emit = true
+}
+
+/*
+========================================================================================
+    FUNCTIONS
+========================================================================================
+*/
+
+//
+// Generate version string
+//
+def getWorkflowVersion() {
+    String version_string = ""
+    if (workflow.manifest.version) {
+        def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
+        version_string += "${prefix_v}${workflow.manifest.version}"
+    }
+
+    if (workflow.commitId) {
+        def git_shortsha = workflow.commitId.substring(0, 7)
+        version_string += "-g${git_shortsha}"
+    }
+
+    return version_string
+}
+
+//
+// Dump pipeline parameters to a JSON file
+//
+def dumpParametersToJSON(outdir) {
+    def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
+    def filename   = "params_${timestamp}.json"
+    def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
+    def jsonStr    = JsonOutput.toJson(params)
+    temp_pf.text   = JsonOutput.prettyPrint(jsonStr)
+
+    FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json")
+    temp_pf.delete()
+}
+
+//
+// When running with -profile conda, warn if channels have not been set-up appropriately
+//
+def checkCondaChannels() {
+    Yaml parser = new Yaml()
+    def channels = []
+    try {
+        def config = parser.load("conda config --show channels".execute().text)
+        channels = config.channels
+    } catch(NullPointerException | IOException e) {
+        log.warn "Could not verify conda channel configuration."
+        return
+    }
+
+    // Check that all channels are present
+    // This channel list is ordered by required channel priority.
+    def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults']
+    def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
+
+    // Check that they are in the right order
+    def channel_priority_violation = false
+    def n = required_channels_in_order.size()
+    for (int i = 0; i < n - 1; i++) {
+        channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1]))
+    }
+
+    if (channels_missing | channel_priority_violation) {
+        log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
+            "  There is a problem with your Conda configuration!\n\n" +
+            "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
+            "  Please refer to https://bioconda.github.io/\n" +
+            "  The observed channel order is \n" +
+            "  ${channels}\n" +
+            "  but the following channel order is required:\n" +
+            "  ${required_channels_in_order}\n" +
+            "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
new file mode 100644
index 00000000..e5c3a0a8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
@@ -0,0 +1,38 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NEXTFLOW_PIPELINE"
+description: Subworkflow with functionality that may be useful for any Nextflow pipeline
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - version
+components: []
+input:
+  - print_version:
+      type: boolean
+      description: |
+        Print the version of the pipeline and exit
+  - dump_parameters:
+      type: boolean
+      description: |
+        Dump the parameters of the pipeline to a JSON file
+  - output_directory:
+      type: directory
+      description: Path to output dir to write JSON file to.
+      pattern: "results/"
+  - check_conda_channel:
+      type: boolean
+      description: |
+        Check if the conda channel priority is correct.
+output:
+  - dummy_emit:
+      type: boolean
+      description: |
+        Dummy emit to make nf-core subworkflows lint happy
+authors:
+  - "@adamrtalbot"
+  - "@drpatelh"
+maintainers:
+  - "@adamrtalbot"
+  - "@drpatelh"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
new file mode 100644
index 00000000..8ed4310c
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
@@ -0,0 +1,54 @@
+
+nextflow_function {
+
+    name "Test Functions"
+    script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf"
+    config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config"
+    tag 'subworkflows'
+    tag 'utils_nextflow_pipeline'
+    tag 'subworkflows/utils_nextflow_pipeline'
+
+    test("Test Function getWorkflowVersion") {
+
+        function "getWorkflowVersion"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function dumpParametersToJSON") {
+
+        function "dumpParametersToJSON"
+
+        when {
+            function {
+                """
+                // define inputs of the function here. Example:
+                input[0] = "$outputDir"
+                """.stripIndent()
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success }
+            )
+        }
+    }
+
+    test("Test Function checkCondaChannels") {
+
+        function "checkCondaChannels"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
new file mode 100644
index 00000000..db2030f8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
@@ -0,0 +1,12 @@
+{
+    "Test Function getWorkflowVersion": {
+        "content": [
+            "v9.9.9"
+        ],
+        "timestamp": "2024-01-19T11:32:36.031083"
+    },
+    "Test Function checkCondaChannels": {
+        "content": null,
+        "timestamp": "2024-01-19T11:32:50.456"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
new file mode 100644
index 00000000..f7c54bc6
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
@@ -0,0 +1,123 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NEXTFLOW_PIPELINE"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config"
+    workflow "UTILS_NEXTFLOW_PIPELINE"
+    tag 'subworkflows'
+    tag 'utils_nextflow_pipeline'
+    tag 'subworkflows/utils_nextflow_pipeline'
+
+    test("Should run no inputs") {
+
+        when {
+            params {
+                outdir = "tests/results"
+            }
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = false
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = print_version
+                input[1] = dump_parameters
+                input[2] = outdir
+                input[3] = check_conda_channels
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should print version") {
+
+        when {
+            params {
+                outdir = "tests/results"
+            }
+            workflow {
+                """
+                print_version        = true
+                dump_parameters      = false
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = print_version
+                input[1] = dump_parameters
+                input[2] = outdir
+                input[3] = check_conda_channels
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.stdout.contains("nextflow_workflow v9.9.9") }
+            )
+        }
+    }
+
+    test("Should dump params") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = true
+                outdir               = params.outdir
+                check_conda_channels = false
+
+                input[0] = false
+                input[1] = true
+                input[2] = params.outdir
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should not create params JSON if no output directory") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = true
+                outdir               = params.outdir
+                check_conda_channels = false
+
+                input[0] = false
+                input[1] = true
+                input[2] = null
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
new file mode 100644
index 00000000..53574ffe
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
@@ -0,0 +1,9 @@
+manifest {
+    name            = 'nextflow_workflow'
+    author          = """nf-core"""
+    homePage        = 'https://127.0.0.1'
+    description     = """Dummy pipeline"""
+    nextflowVersion  = '!>=23.04.0'
+    version         = '9.9.9'
+    doi             = 'https://doi.org/10.5281/zenodo.5070524'
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
new file mode 100644
index 00000000..f8476112
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nextflow_pipeline:
+  - subworkflows/nf-core/utils_nextflow_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
new file mode 100644
index 00000000..a8b55d6f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -0,0 +1,440 @@
+//
+// Subworkflow with utility functions specific to the nf-core pipeline template
+//
+
+import org.yaml.snakeyaml.Yaml
+import nextflow.extension.FilesEx
+
+/*
+========================================================================================
+    SUBWORKFLOW DEFINITION
+========================================================================================
+*/
+
+workflow UTILS_NFCORE_PIPELINE {
+
+    take:
+    nextflow_cli_args
+
+    main:
+    valid_config = checkConfigProvided()
+    checkProfileProvided(nextflow_cli_args)
+
+    emit:
+    valid_config
+}
+
+/*
+========================================================================================
+    FUNCTIONS
+========================================================================================
+*/
+
+//
+//  Warn if a -profile or Nextflow config has not been provided to run the pipeline
+//
+def checkConfigProvided() {
+    valid_config = true
+    if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
+        log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
+            "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
+            "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
+            "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
+            "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
+            "Please refer to the quick start section and usage docs for the pipeline.\n "
+        valid_config = false
+    }
+    return valid_config
+}
+
+//
+// Exit pipeline if --profile contains spaces
+//
+def checkProfileProvided(nextflow_cli_args) {
+    if (workflow.profile.endsWith(',')) {
+        error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" +
+            "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+    }
+    if (nextflow_cli_args[0]) {
+        log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" +
+            "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+    }
+}
+
+//
+// Citation string for pipeline
+//
+def workflowCitation() {
+    return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
+        "* The pipeline\n" +
+        "  ${workflow.manifest.doi}\n\n" +
+        "* The nf-core framework\n" +
+        "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
+        "* Software dependencies\n" +
+        "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
+}
+
+//
+// Generate workflow version string
+//
+def getWorkflowVersion() {
+    String version_string = ""
+    if (workflow.manifest.version) {
+        def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
+        version_string += "${prefix_v}${workflow.manifest.version}"
+    }
+
+    if (workflow.commitId) {
+        def git_shortsha = workflow.commitId.substring(0, 7)
+        version_string += "-g${git_shortsha}"
+    }
+
+    return version_string
+}
+
+//
+// Get software versions for pipeline
+//
+def processVersionsFromYAML(yaml_file) {
+    Yaml yaml = new Yaml()
+    versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] }
+    return yaml.dumpAsMap(versions).trim()
+}
+
+//
+// Get workflow version for pipeline
+//
+def workflowVersionToYAML() {
+    return """
+    Workflow:
+        $workflow.manifest.name: ${getWorkflowVersion()}
+        Nextflow: $workflow.nextflow.version
+    """.stripIndent().trim()
+}
+
+//
+// Get channel of software versions used in pipeline in YAML format
+//
+def softwareVersionsToYAML(ch_versions) {
+    return ch_versions
+                .unique()
+                .map { processVersionsFromYAML(it) }
+                .unique()
+                .mix(Channel.of(workflowVersionToYAML()))
+}
+
+//
+// Get workflow summary for MultiQC
+//
+def paramsSummaryMultiqc(summary_params) {
+    def summary_section = ''
+    for (group in summary_params.keySet()) {
+        def group_params = summary_params.get(group)  // This gets the parameters of that particular group
+        if (group_params) {
+            summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
+            summary_section += "    <dl class=\"dl-horizontal\">\n"
+            for (param in group_params.keySet()) {
+                summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
+            }
+            summary_section += "    </dl>\n"
+        }
+    }
+
+    String yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n"
+    yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
+    yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
+    yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
+    yaml_file_text        += "plot_type: 'html'\n"
+    yaml_file_text        += "data: |\n"
+    yaml_file_text        += "${summary_section}"
+
+    return yaml_file_text
+}
+
+//
+// nf-core logo
+//
+def nfCoreLogo(monochrome_logs=true) {
+    Map colors = logColours(monochrome_logs)
+    String.format(
+        """\n
+        ${dashedLine(monochrome_logs)}
+                                                ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
+        ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
+        ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
+        ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
+                                                ${colors.green}`._,._,\'${colors.reset}
+        ${colors.purple}  ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset}
+        ${dashedLine(monochrome_logs)}
+        """.stripIndent()
+    )
+}
+
+//
+// Return dashed line
+//
+def dashedLine(monochrome_logs=true) {
+    Map colors = logColours(monochrome_logs)
+    return "-${colors.dim}----------------------------------------------------${colors.reset}-"
+}
+
+//
+// ANSII colours used for terminal logging
+//
+def logColours(monochrome_logs=true) {
+    Map colorcodes = [:]
+
+    // Reset / Meta
+    colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
+    colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
+    colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
+    colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
+    colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
+    colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
+    colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
+
+    // Regular Colors
+    colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
+    colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
+    colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
+    colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
+    colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
+    colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
+    colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
+    colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
+
+    // Bold
+    colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
+    colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
+    colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
+    colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
+    colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
+    colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
+    colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
+    colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
+
+    // Underline
+    colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
+    colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
+    colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
+    colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
+    colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
+    colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
+    colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
+    colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
+
+    // High Intensity
+    colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
+    colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
+    colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
+    colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
+    colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
+    colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
+    colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
+    colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
+
+    // Bold High Intensity
+    colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
+    colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
+    colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
+    colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
+    colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
+    colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
+    colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
+    colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
+
+    return colorcodes
+}
+
+//
+// Attach the multiqc report to email
+//
+def attachMultiqcReport(multiqc_report) {
+    def mqc_report = null
+    try {
+        if (workflow.success) {
+            mqc_report = multiqc_report.getVal()
+            if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
+                if (mqc_report.size() > 1) {
+                    log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
+                }
+                mqc_report = mqc_report[0]
+            }
+        }
+    } catch (all) {
+        if (multiqc_report) {
+            log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
+        }
+    }
+    return mqc_report
+}
+
+//
+// Construct and send completion email
+//
+def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) {
+
+    // Set up the e-mail variables
+    def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
+    if (!workflow.success) {
+        subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
+    }
+
+    def summary = [:]
+    for (group in summary_params.keySet()) {
+        summary << summary_params[group]
+    }
+
+    def misc_fields = [:]
+    misc_fields['Date Started']              = workflow.start
+    misc_fields['Date Completed']            = workflow.complete
+    misc_fields['Pipeline script file path'] = workflow.scriptFile
+    misc_fields['Pipeline script hash ID']   = workflow.scriptId
+    if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
+    if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
+    if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
+    misc_fields['Nextflow Version']           = workflow.nextflow.version
+    misc_fields['Nextflow Build']             = workflow.nextflow.build
+    misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
+
+    def email_fields = [:]
+    email_fields['version']      = getWorkflowVersion()
+    email_fields['runName']      = workflow.runName
+    email_fields['success']      = workflow.success
+    email_fields['dateComplete'] = workflow.complete
+    email_fields['duration']     = workflow.duration
+    email_fields['exitStatus']   = workflow.exitStatus
+    email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+    email_fields['errorReport']  = (workflow.errorReport ?: 'None')
+    email_fields['commandLine']  = workflow.commandLine
+    email_fields['projectDir']   = workflow.projectDir
+    email_fields['summary']      = summary << misc_fields
+
+    // On success try attach the multiqc report
+    def mqc_report = attachMultiqcReport(multiqc_report)
+
+    // Check if we are only sending emails on failure
+    def email_address = email
+    if (!email && email_on_fail && !workflow.success) {
+        email_address = email_on_fail
+    }
+
+    // Render the TXT template
+    def engine       = new groovy.text.GStringTemplateEngine()
+    def tf           = new File("${workflow.projectDir}/assets/email_template.txt")
+    def txt_template = engine.createTemplate(tf).make(email_fields)
+    def email_txt    = txt_template.toString()
+
+    // Render the HTML template
+    def hf            = new File("${workflow.projectDir}/assets/email_template.html")
+    def html_template = engine.createTemplate(hf).make(email_fields)
+    def email_html    = html_template.toString()
+
+    // Render the sendmail template
+    def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
+    def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
+    def sf                     = new File("${workflow.projectDir}/assets/sendmail_template.txt")
+    def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
+    def sendmail_html          = sendmail_template.toString()
+
+    // Send the HTML e-mail
+    Map colors = logColours(monochrome_logs)
+    if (email_address) {
+        try {
+            if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
+            // Try to send HTML e-mail using sendmail
+            def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
+            sendmail_tf.withWriter { w -> w << sendmail_html }
+            [ 'sendmail', '-t' ].execute() << sendmail_html
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
+        } catch (all) {
+            // Catch failures and try with plaintext
+            def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
+            mail_cmd.execute() << email_html
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
+        }
+    }
+
+    // Write summary e-mail HTML to a file
+    def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
+    output_hf.withWriter { w -> w << email_html }
+    FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html");
+    output_hf.delete()
+
+    // Write summary e-mail TXT to a file
+    def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
+    output_tf.withWriter { w -> w << email_txt }
+    FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt");
+    output_tf.delete()
+}
+
+//
+// Print pipeline summary on completion
+//
+def completionSummary(monochrome_logs=true) {
+    Map colors = logColours(monochrome_logs)
+    if (workflow.success) {
+        if (workflow.stats.ignoredCount == 0) {
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
+        } else {
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
+        }
+    } else {
+        log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
+    }
+}
+
+//
+// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack
+//
+def imNotification(summary_params, hook_url) {
+    def summary = [:]
+    for (group in summary_params.keySet()) {
+        summary << summary_params[group]
+    }
+
+    def misc_fields = [:]
+    misc_fields['start']                                = workflow.start
+    misc_fields['complete']                             = workflow.complete
+    misc_fields['scriptfile']                           = workflow.scriptFile
+    misc_fields['scriptid']                             = workflow.scriptId
+    if (workflow.repository) misc_fields['repository']  = workflow.repository
+    if (workflow.commitId)   misc_fields['commitid']    = workflow.commitId
+    if (workflow.revision)   misc_fields['revision']    = workflow.revision
+    misc_fields['nxf_version']                          = workflow.nextflow.version
+    misc_fields['nxf_build']                            = workflow.nextflow.build
+    misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
+
+    def msg_fields = [:]
+    msg_fields['version']      = getWorkflowVersion()
+    msg_fields['runName']      = workflow.runName
+    msg_fields['success']      = workflow.success
+    msg_fields['dateComplete'] = workflow.complete
+    msg_fields['duration']     = workflow.duration
+    msg_fields['exitStatus']   = workflow.exitStatus
+    msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+    msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
+    msg_fields['commandLine']  = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "")
+    msg_fields['projectDir']   = workflow.projectDir
+    msg_fields['summary']      = summary << misc_fields
+
+    // Render the JSON template
+    def engine       = new groovy.text.GStringTemplateEngine()
+    // Different JSON depending on the service provider
+    // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format
+    def json_path     = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json"
+    def hf            = new File("${workflow.projectDir}/assets/${json_path}")
+    def json_template = engine.createTemplate(hf).make(msg_fields)
+    def json_message  = json_template.toString()
+
+    // POST
+    def post = new URL(hook_url).openConnection();
+    post.setRequestMethod("POST")
+    post.setDoOutput(true)
+    post.setRequestProperty("Content-Type", "application/json")
+    post.getOutputStream().write(json_message.getBytes("UTF-8"));
+    def postRC = post.getResponseCode();
+    if (! postRC.equals(200)) {
+        log.warn(post.getErrorStream().getText());
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
new file mode 100644
index 00000000..d08d2434
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
@@ -0,0 +1,24 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NFCORE_PIPELINE"
+description: Subworkflow with utility functions specific to the nf-core pipeline template
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - version
+components: []
+input:
+  - nextflow_cli_args:
+      type: list
+      description: |
+        Nextflow CLI positional arguments
+output:
+  - success:
+      type: boolean
+      description: |
+        Dummy output to indicate success
+authors:
+  - "@adamrtalbot"
+maintainers:
+  - "@adamrtalbot"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
new file mode 100644
index 00000000..1dc317f8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
@@ -0,0 +1,134 @@
+
+nextflow_function {
+
+    name "Test Functions"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "utils_nfcore_pipeline"
+    tag "subworkflows/utils_nfcore_pipeline"
+
+    test("Test Function checkConfigProvided") {
+
+        function "checkConfigProvided"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function checkProfileProvided") {
+
+        function "checkProfileProvided"
+
+        when {
+            function {
+                """
+                input[0] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function workflowCitation") {
+
+        function "workflowCitation"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function nfCoreLogo") {
+
+        function "nfCoreLogo"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function dashedLine") {
+
+        function "dashedLine"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function without logColours") {
+
+        function "logColours"
+
+        when {
+            function {
+                """
+                input[0] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function with logColours") {
+        function "logColours"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
new file mode 100644
index 00000000..10f948e6
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
@@ -0,0 +1,138 @@
+{
+    "Test Function checkProfileProvided": {
+        "content": null,
+        "timestamp": "2024-02-09T15:43:55.145717"
+    },
+    "Test Function checkConfigProvided": {
+        "content": [
+            true
+        ],
+        "timestamp": "2024-01-19T11:34:13.548431224"
+    },
+    "Test Function nfCoreLogo": {
+        "content": [
+            "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n                                        \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m        ___     __   __   __   ___     \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \u001b[0;33m}  {\u001b[0m\n\u001b[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \u001b[0;32m\\`-._,-`-,\u001b[0m\n                                        \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m  nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n"
+        ],
+        "timestamp": "2024-01-19T11:34:38.840454873"
+    },
+    "Test Function workflowCitation": {
+        "content": [
+            "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n  https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n  https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n  https://github.com/nextflow_workflow/blob/master/CITATIONS.md"
+        ],
+        "timestamp": "2024-01-19T11:34:22.24352016"
+    },
+    "Test Function without logColours": {
+        "content": [
+            {
+                "reset": "",
+                "bold": "",
+                "dim": "",
+                "underlined": "",
+                "blink": "",
+                "reverse": "",
+                "hidden": "",
+                "black": "",
+                "red": "",
+                "green": "",
+                "yellow": "",
+                "blue": "",
+                "purple": "",
+                "cyan": "",
+                "white": "",
+                "bblack": "",
+                "bred": "",
+                "bgreen": "",
+                "byellow": "",
+                "bblue": "",
+                "bpurple": "",
+                "bcyan": "",
+                "bwhite": "",
+                "ublack": "",
+                "ured": "",
+                "ugreen": "",
+                "uyellow": "",
+                "ublue": "",
+                "upurple": "",
+                "ucyan": "",
+                "uwhite": "",
+                "iblack": "",
+                "ired": "",
+                "igreen": "",
+                "iyellow": "",
+                "iblue": "",
+                "ipurple": "",
+                "icyan": "",
+                "iwhite": "",
+                "biblack": "",
+                "bired": "",
+                "bigreen": "",
+                "biyellow": "",
+                "biblue": "",
+                "bipurple": "",
+                "bicyan": "",
+                "biwhite": ""
+            }
+        ],
+        "timestamp": "2024-01-19T11:35:04.418416984"
+    },
+    "Test Function dashedLine": {
+        "content": [
+            "-\u001b[2m----------------------------------------------------\u001b[0m-"
+        ],
+        "timestamp": "2024-01-19T11:34:55.420000755"
+    },
+    "Test Function with logColours": {
+        "content": [
+            {
+                "reset": "\u001b[0m",
+                "bold": "\u001b[1m",
+                "dim": "\u001b[2m",
+                "underlined": "\u001b[4m",
+                "blink": "\u001b[5m",
+                "reverse": "\u001b[7m",
+                "hidden": "\u001b[8m",
+                "black": "\u001b[0;30m",
+                "red": "\u001b[0;31m",
+                "green": "\u001b[0;32m",
+                "yellow": "\u001b[0;33m",
+                "blue": "\u001b[0;34m",
+                "purple": "\u001b[0;35m",
+                "cyan": "\u001b[0;36m",
+                "white": "\u001b[0;37m",
+                "bblack": "\u001b[1;30m",
+                "bred": "\u001b[1;31m",
+                "bgreen": "\u001b[1;32m",
+                "byellow": "\u001b[1;33m",
+                "bblue": "\u001b[1;34m",
+                "bpurple": "\u001b[1;35m",
+                "bcyan": "\u001b[1;36m",
+                "bwhite": "\u001b[1;37m",
+                "ublack": "\u001b[4;30m",
+                "ured": "\u001b[4;31m",
+                "ugreen": "\u001b[4;32m",
+                "uyellow": "\u001b[4;33m",
+                "ublue": "\u001b[4;34m",
+                "upurple": "\u001b[4;35m",
+                "ucyan": "\u001b[4;36m",
+                "uwhite": "\u001b[4;37m",
+                "iblack": "\u001b[0;90m",
+                "ired": "\u001b[0;91m",
+                "igreen": "\u001b[0;92m",
+                "iyellow": "\u001b[0;93m",
+                "iblue": "\u001b[0;94m",
+                "ipurple": "\u001b[0;95m",
+                "icyan": "\u001b[0;96m",
+                "iwhite": "\u001b[0;97m",
+                "biblack": "\u001b[1;90m",
+                "bired": "\u001b[1;91m",
+                "bigreen": "\u001b[1;92m",
+                "biyellow": "\u001b[1;93m",
+                "biblue": "\u001b[1;94m",
+                "bipurple": "\u001b[1;95m",
+                "bicyan": "\u001b[1;96m",
+                "biwhite": "\u001b[1;97m"
+            }
+        ],
+        "timestamp": "2024-01-19T11:35:13.436366565"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
new file mode 100644
index 00000000..8940d32d
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
@@ -0,0 +1,29 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NFCORE_PIPELINE"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
+    workflow "UTILS_NFCORE_PIPELINE"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "utils_nfcore_pipeline"
+    tag "subworkflows/utils_nfcore_pipeline"
+
+    test("Should run without failures") {
+
+        when {
+            workflow {
+                """
+                input[0] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
new file mode 100644
index 00000000..d07ce54c
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
@@ -0,0 +1,15 @@
+{
+    "Should run without failures": {
+        "content": [
+            {
+                "0": [
+                    true
+                ],
+                "valid_config": [
+                    true
+                ]
+            }
+        ],
+        "timestamp": "2024-01-19T11:35:22.538940073"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
new file mode 100644
index 00000000..d0a926bf
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
@@ -0,0 +1,9 @@
+manifest {
+    name            = 'nextflow_workflow'
+    author          = """nf-core"""
+    homePage        = 'https://127.0.0.1'
+    description     = """Dummy pipeline"""
+    nextflowVersion  = '!>=23.04.0'
+    version         = '9.9.9'
+    doi             = 'https://doi.org/10.5281/zenodo.5070524'
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
new file mode 100644
index 00000000..ac8523c9
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nfcore_pipeline:
+  - subworkflows/nf-core/utils_nfcore_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
new file mode 100644
index 00000000..2585b65d
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
@@ -0,0 +1,62 @@
+//
+// Subworkflow that uses the nf-validation plugin to render help text and parameter summary
+//
+
+/*
+========================================================================================
+    IMPORT NF-VALIDATION PLUGIN
+========================================================================================
+*/
+
+include { paramsHelp         } from 'plugin/nf-validation'
+include { paramsSummaryLog   } from 'plugin/nf-validation'
+include { validateParameters } from 'plugin/nf-validation'
+
+/*
+========================================================================================
+    SUBWORKFLOW DEFINITION
+========================================================================================
+*/
+
+workflow UTILS_NFVALIDATION_PLUGIN {
+
+    take:
+    print_help       // boolean: print help
+    workflow_command //  string: default commmand used to run pipeline
+    pre_help_text    //  string: string to be printed before help text and summary log
+    post_help_text   //  string: string to be printed after help text and summary log
+    validate_params  // boolean: validate parameters
+    schema_filename  //    path: JSON schema file, null to use default value
+
+    main:
+
+    log.debug "Using schema file: ${schema_filename}"
+
+    // Default values for strings
+    pre_help_text    = pre_help_text    ?: ''
+    post_help_text   = post_help_text   ?: ''
+    workflow_command = workflow_command ?: ''
+
+    //
+    // Print help message if needed
+    //
+    if (print_help) {
+        log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text
+        System.exit(0)
+    }
+
+    //
+    // Print parameter summary to stdout
+    //
+    log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text
+
+    //
+    // Validate parameters relative to the parameter JSON schema
+    //
+    if (validate_params){
+        validateParameters(parameters_schema: schema_filename)
+    }
+
+    emit:
+    dummy_emit = true
+}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
new file mode 100644
index 00000000..3d4a6b04
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
@@ -0,0 +1,44 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NFVALIDATION_PLUGIN"
+description: Use nf-validation to initiate and validate a pipeline
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - validation
+components: []
+input:
+  - print_help:
+      type: boolean
+      description: |
+        Print help message and exit
+  - workflow_command:
+      type: string
+      description: |
+        The command to run the workflow e.g. "nextflow run main.nf"
+  - pre_help_text:
+      type: string
+      description: |
+        Text to print before the help message
+  - post_help_text:
+      type: string
+      description: |
+        Text to print after the help message
+  - validate_params:
+      type: boolean
+      description: |
+        Validate the parameters and error if invalid.
+  - schema_filename:
+      type: string
+      description: |
+        The filename of the schema to validate against.
+output:
+  - dummy_emit:
+      type: boolean
+      description: |
+        Dummy emit to make nf-core subworkflows lint happy
+authors:
+  - "@adamrtalbot"
+maintainers:
+  - "@adamrtalbot"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
new file mode 100644
index 00000000..517ee54e
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
@@ -0,0 +1,200 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NFVALIDATION_PLUGIN"
+    script "../main.nf"
+    workflow "UTILS_NFVALIDATION_PLUGIN"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "plugin/nf-validation"
+    tag "'plugin/nf-validation'"
+    tag "utils_nfvalidation_plugin"
+    tag "subworkflows/utils_nfvalidation_plugin"
+
+    test("Should run nothing") {
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+
+            workflow {
+                """
+                help             = false
+                workflow_command = null
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should run help") {
+
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+            workflow {
+                """
+                help             = true
+                workflow_command = null
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.exitStatus == 0 },
+                { assert workflow.stdout.any { it.contains('Input/output options') } },
+                { assert workflow.stdout.any { it.contains('--outdir') } }
+            )
+        }
+    }
+
+    test("Should run help with command") {
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+            workflow {
+                """
+                help             = true
+                workflow_command = "nextflow run noorg/doesntexist"
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.exitStatus == 0 },
+                { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } },
+                { assert workflow.stdout.any { it.contains('Input/output options') } },
+                { assert workflow.stdout.any { it.contains('--outdir') } }
+            )
+        }
+    }
+
+    test("Should run help with extra text") {
+
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+            }
+            workflow {
+                """
+                help             = true
+                workflow_command = "nextflow run noorg/doesntexist"
+                pre_help_text    = "pre-help-text"
+                post_help_text   = "post-help-text"
+                validate_params  = false
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.exitStatus == 0 },
+                { assert workflow.stdout.any { it.contains('pre-help-text') } },
+                { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } },
+                { assert workflow.stdout.any { it.contains('Input/output options') } },
+                { assert workflow.stdout.any { it.contains('--outdir') } },
+                { assert workflow.stdout.any { it.contains('post-help-text') } }
+            )
+        }
+    }
+
+    test("Should validate params") {
+
+        when {
+
+            params {
+                monochrome_logs = true
+                test_data       = ''
+                outdir          = 1
+            }
+            workflow {
+                """
+                help             = false
+                workflow_command = null
+                pre_help_text    = null
+                post_help_text   = null
+                validate_params  = true
+                schema_filename  = "$moduleTestDir/nextflow_schema.json"
+
+                input[0] = help
+                input[1] = workflow_command
+                input[2] = pre_help_text
+                input[3] = post_help_text
+                input[4] = validate_params
+                input[5] = schema_filename
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.failed },
+                { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } }
+            )
+        }
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
new file mode 100644
index 00000000..7626c1c9
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
@@ -0,0 +1,96 @@
+{
+    "$schema": "http://json-schema.org/draft-07/schema",
+    "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json",
+    "title": ". pipeline parameters",
+    "description": "",
+    "type": "object",
+    "definitions": {
+        "input_output_options": {
+            "title": "Input/output options",
+            "type": "object",
+            "fa_icon": "fas fa-terminal",
+            "description": "Define where the pipeline should find input data and save output data.",
+            "required": ["outdir"],
+            "properties": {
+                "validate_params": {
+                    "type": "boolean",
+                    "description": "Validate parameters?",
+                    "default": true,
+                    "hidden": true
+                },
+                "outdir": {
+                    "type": "string",
+                    "format": "directory-path",
+                    "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.",
+                    "fa_icon": "fas fa-folder-open"
+                },
+                "test_data_base": {
+                    "type": "string",
+                    "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules",
+                    "description": "Base for test data directory",
+                    "hidden": true
+                },
+                "test_data": {
+                    "type": "string",
+                    "description": "Fake test data param",
+                    "hidden": true
+                }
+            }
+        },
+        "generic_options": {
+            "title": "Generic options",
+            "type": "object",
+            "fa_icon": "fas fa-file-import",
+            "description": "Less common options for the pipeline, typically set in a config file.",
+            "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
+            "properties": {
+                "help": {
+                    "type": "boolean",
+                    "description": "Display help text.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "version": {
+                    "type": "boolean",
+                    "description": "Display version and exit.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "logo": {
+                    "type": "boolean",
+                    "default": true,
+                    "description": "Display nf-core logo in console output.",
+                    "fa_icon": "fas fa-image",
+                    "hidden": true
+                },
+                "singularity_pull_docker_container": {
+                    "type": "boolean",
+                    "description": "Pull Singularity container from Docker?",
+                    "hidden": true
+                },
+                "publish_dir_mode": {
+                    "type": "string",
+                    "default": "copy",
+                    "description": "Method used to save pipeline results to output directory.",
+                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
+                    "fa_icon": "fas fa-copy",
+                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
+                    "hidden": true
+                },
+                "monochrome_logs": {
+                    "type": "boolean",
+                    "description": "Use monochrome_logs",
+                    "hidden": true
+                }
+            }
+        }
+    },
+    "allOf": [
+        {
+            "$ref": "#/definitions/input_output_options"
+        },
+        {
+            "$ref": "#/definitions/generic_options"
+        }
+    ]
+}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml
new file mode 100644
index 00000000..60b1cfff
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nfvalidation_plugin:
+  - subworkflows/nf-core/utils_nfvalidation_plugin/**
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 95194afa..9a9ceddf 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -1,54 +1,15 @@
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    PRINT PARAMS SUMMARY
+    IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation'
-
-def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs)
-def citation = '\n' + WorkflowMain.citation(workflow) + '\n'
-def summary_params = paramsSummaryMap(workflow)
-
-// Print parameter summary log to screen
-log.info logo + paramsSummaryLog(workflow) + citation
-
-WorkflowSmrnaseq.initialise(params, log)
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    CONFIG FILES
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-ch_multiqc_config          = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-ch_multiqc_custom_config   = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty()
-ch_multiqc_logo            = params.multiqc_logo   ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty()
-ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT LOCAL MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
-//
-include { INPUT_CHECK } from '../subworkflows/local/input_check'
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT NF-CORE MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// MODULE: Installed directly from nf-core/modules
-//
-include { FASTQC                      } from '../modules/nf-core/fastqc/main'
-include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
-include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
+include { FASTQC                 } from '../modules/nf-core/fastqc/main'
+include { MULTIQC                } from '../modules/nf-core/multiqc/main'
+include { paramsSummaryMap       } from 'plugin/nf-validation'
+include { paramsSummaryMultiqc   } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_smrnaseq_pipeline'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -56,50 +17,45 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-// Info required for completion email and summary
-def multiqc_report = []
-
 workflow SMRNASEQ {
 
-    ch_versions = Channel.empty()
+    take:
+    ch_samplesheet // channel: samplesheet read in from --input
 
-    //
-    // SUBWORKFLOW: Read in samplesheet, validate and stage input files
-    //
-    INPUT_CHECK (
-        file(params.input)
-    )
-    ch_versions = ch_versions.mix(INPUT_CHECK.out.versions)
-    // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input")
-    // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/
-    // ! There is currently no tooling to help you write a sample sheet schema
+    main:
+
+    ch_versions = Channel.empty()
+    ch_multiqc_files = Channel.empty()
 
     //
     // MODULE: Run FastQC
     //
     FASTQC (
-        INPUT_CHECK.out.reads
+        ch_samplesheet
     )
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
     ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
-    CUSTOM_DUMPSOFTWAREVERSIONS (
-        ch_versions.unique().collectFile(name: 'collated_versions.yml')
-    )
+    //
+    // Collate and save software versions
+    //
+    softwareVersionsToYAML(ch_versions)
+        .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true)
+        .set { ch_collated_versions }
 
     //
     // MODULE: MultiQC
     //
-    workflow_summary    = WorkflowSmrnaseq.paramsSummaryMultiqc(workflow, summary_params)
-    ch_workflow_summary = Channel.value(workflow_summary)
-
-    methods_description    = WorkflowSmrnaseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params)
-    ch_methods_description = Channel.value(methods_description)
-
-    ch_multiqc_files = Channel.empty()
-    ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml'))
-    ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
-    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_config                     = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+    ch_multiqc_custom_config              = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty()
+    ch_multiqc_logo                       = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty()
+    summary_params                        = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
+    ch_workflow_summary                   = Channel.value(paramsSummaryMultiqc(summary_params))
+    ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
+    ch_methods_description                = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description))
+    ch_multiqc_files                      = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files                      = ch_multiqc_files.mix(ch_collated_versions)
+    ch_multiqc_files                      = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false))
 
     MULTIQC (
         ch_multiqc_files.collect(),
@@ -107,31 +63,10 @@ workflow SMRNASEQ {
         ch_multiqc_custom_config.toList(),
         ch_multiqc_logo.toList()
     )
-    multiqc_report = MULTIQC.out.report.toList()
-}
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    COMPLETION EMAIL AND SUMMARY
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-workflow.onComplete {
-    if (params.email || params.email_on_fail) {
-        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
-    }
-    NfcoreTemplate.dump_parameters(workflow, params)
-    NfcoreTemplate.summary(workflow, params, log)
-    if (params.hook_url) {
-        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
-    }
-}
 
-workflow.onError {
-    if (workflow.errorReport.contains("Process requirement exceeds available memory")) {
-        println("🛑 Default resources exceed availability 🛑 ")
-        println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡")
-    }
+    emit:
+    multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
+    versions       = ch_versions                 // channel: [ path(versions.yml) ]
 }
 
 /*

From b27b98b6207bb33f48f0fe31826edfeb967b9fed Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Wed, 21 Feb 2024 11:04:43 +0000
Subject: [PATCH 195/227] add functionality to process archived bowtie index

---
 modules.json                               |  5 +++
 modules/nf-core/untarfiles/environment.yml |  9 ++++
 modules/nf-core/untarfiles/main.nf         | 52 ++++++++++++++++++++++
 modules/nf-core/untarfiles/meta.yml        | 48 ++++++++++++++++++++
 workflows/smrnaseq.nf                      |  9 +++-
 5 files changed, 122 insertions(+), 1 deletion(-)
 create mode 100644 modules/nf-core/untarfiles/environment.yml
 create mode 100644 modules/nf-core/untarfiles/main.nf
 create mode 100644 modules/nf-core/untarfiles/meta.yml

diff --git a/modules.json b/modules.json
index b7261f61..bdad7a91 100644
--- a/modules.json
+++ b/modules.json
@@ -69,6 +69,11 @@
                         "branch": "master",
                         "git_sha": "9e56d7a647fbf6f7e45ef123bc916ad66b6f7c9d",
                         "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
+                    },
+                    "untarfiles": {
+                        "branch": "master",
+                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "installed_by": ["modules"]
                     }
                 }
             },
diff --git a/modules/nf-core/untarfiles/environment.yml b/modules/nf-core/untarfiles/environment.yml
new file mode 100644
index 00000000..e479f80d
--- /dev/null
+++ b/modules/nf-core/untarfiles/environment.yml
@@ -0,0 +1,9 @@
+name: untarfiles
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - conda-forge::sed=4.7
+  - bioconda::grep=3.4
+  - conda-forge::tar=1.34
diff --git a/modules/nf-core/untarfiles/main.nf b/modules/nf-core/untarfiles/main.nf
new file mode 100644
index 00000000..de27e67c
--- /dev/null
+++ b/modules/nf-core/untarfiles/main.nf
@@ -0,0 +1,52 @@
+process UNTARFILES {
+    tag "$archive"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
+        'nf-core/ubuntu:20.04' }"
+
+    input:
+    tuple val(meta), path(archive)
+
+    output:
+    tuple val(meta), path("${prefix}/**") , emit: files
+    path "versions.yml"                   , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args  = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    prefix    = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, ""))
+
+    """
+    mkdir $prefix
+
+    tar \\
+        -C $prefix \\
+        -xavf \\
+        $args \\
+        $archive \\
+        $args2
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    prefix    = task.ext.prefix ?: "${meta.id}"
+    """
+    mkdir $prefix
+    touch ${prefix}/file.txt
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/untarfiles/meta.yml b/modules/nf-core/untarfiles/meta.yml
new file mode 100644
index 00000000..38108826
--- /dev/null
+++ b/modules/nf-core/untarfiles/meta.yml
@@ -0,0 +1,48 @@
+name: untarfiles
+description: Extract files.
+keywords:
+  - untar
+  - uncompress
+  - files
+tools:
+  - untar:
+      description: |
+        Extract tar.gz files.
+      documentation: https://www.gnu.org/software/tar/manual/
+      licence: ["GPL-3.0-or-later"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - archive:
+      type: file
+      description: File to be untar
+      pattern: "*.{tar}.{gz}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - files:
+      type: string
+      description: A list containing references to individual archive files
+      pattern: "*/**"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@matthdsm"
+  - "@jfy133"
+  - "@pinin4fjords"
+maintainers:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@matthdsm"
+  - "@jfy133"
+  - "@pinin4fjords"
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index d86b0280..8c998cdb 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -67,6 +67,7 @@ if (!params.mirgenedb) {
 include { INPUT_CHECK                               } from '../subworkflows/local/input_check'
 include { FASTQ_FASTQC_UMITOOLS_FASTP               } from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp'
 include { FASTP as FASTP_LENGTH_FILTER              } from '../modules/nf-core/fastp'
+include { UNTARFILES as UNTAR_BOWTIE_INDEX          } from '../modules/nf-core/untarfiles'
 include { CONTAMINANT_FILTER                        } from '../subworkflows/local/contaminant_filter'
 include { MIRNA_QUANT                               } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT                              } from '../subworkflows/local/genome_quant'
@@ -162,8 +163,14 @@ workflow SMRNASEQ {
         //Prepare bowtie index, unless specified
         //This needs to be done here as the index is used by GENOME_QUANT
         if(params.bowtie_index) {
-            Channel.fromPath("${params.bowtie_index}**ebwt", checkIfExists: true).ifEmpty{ error "Bowtie1 index directory not found: ${params.bowtie_index}" }.filter { it != null }.set { ch_bowtie_index }
             ch_fasta = Channel.fromPath(params.fasta)
+            if (params.bowtie_index.endsWith(".tar.gz")) {
+                UNTAR_BOWTIE_INDEX ( [ [], params.bowtie_index ]).files.map { it[1] }.set {ch_bowtie_index}
+                ch_versions  = ch_versions.mix(UNTAR_BOWTIE_INDEX.out.versions)
+            } else {
+                Channel.fromPath("${params.bowtie_index}**ebwt", checkIfExists: true).ifEmpty{ error "Bowtie1 index directory not found: ${params.bowtie_index}" }.filter { it != null }.set { ch_bowtie_index }
+            }Channel
+        .fromPath(
         } else {
             INDEX_GENOME ( [ [:], ch_fasta ] )
             ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)

From 6e5888cb256ba597f1d7ce2594799e7cdbee6594 Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Wed, 21 Feb 2024 11:06:07 +0000
Subject: [PATCH 196/227] adjust test config with bowtie index

---
 conf/test_index.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/test_index.config b/conf/test_index.config
index 64ae5766..bb9f4707 100644
--- a/conf/test_index.config
+++ b/conf/test_index.config
@@ -23,7 +23,7 @@ params {
 
     input            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/samplesheet/v2.0/samplesheet.csv'
     fasta            = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.fa'
-    bowtie_index     = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/'
+    bowtie_index     = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/bowtie_index.tar.gz'
 
     mirtrace_species         = 'hsa'
     protocol                 = 'illumina'

From bf78343b0753f0a5dde1171174e05f01331d457a Mon Sep 17 00:00:00 2001
From: Christopher Mohr <contact.cmohr@gmail.com>
Date: Wed, 21 Feb 2024 11:16:13 +0000
Subject: [PATCH 197/227] fix syntax error

---
 workflows/smrnaseq.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 8c998cdb..39e402f8 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -169,8 +169,7 @@ workflow SMRNASEQ {
                 ch_versions  = ch_versions.mix(UNTAR_BOWTIE_INDEX.out.versions)
             } else {
                 Channel.fromPath("${params.bowtie_index}**ebwt", checkIfExists: true).ifEmpty{ error "Bowtie1 index directory not found: ${params.bowtie_index}" }.filter { it != null }.set { ch_bowtie_index }
-            }Channel
-        .fromPath(
+            }
         } else {
             INDEX_GENOME ( [ [:], ch_fasta ] )
             ch_versions = ch_versions.mix(INDEX_GENOME.out.versions)

From 318e05e2b415c0c60a73fdb173223ec6441ccb4a Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:03:20 +0000
Subject: [PATCH 198/227] Remove leftover CI cleanup

---
 .github/workflows/ci.yml | 3 ---
 1 file changed, 3 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 6f52e5a1..7488431b 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -45,6 +45,3 @@ jobs:
       - name: Run pipeline with test data
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.profile }},docker --outdir ./results
-
-      - name: Free Disk Space for Phil - Because Science!
-        uses: jlumbroso/free-disk-space@v1.3.1

From 7082993151f03c4660535b53d2eafc4869922ce4 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:11:15 +0000
Subject: [PATCH 199/227] Adjust function calls

---
 workflows/smrnaseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index d133a749..d4e54533 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -243,7 +243,7 @@ workflow SMRNASEQ {
     //
     if (!params.skip_multiqc) {
         workflow_summary    = WorkflowSmrnaseq.paramsSummaryMultiqc(workflow, summary_params)
-        ch_workflow_summary = Channel.value(workflow_summary)
+        ch_workflow_summary = Channel.value(paramsSummaryMultiqc(workflow_summary))
 
         methods_description    = WorkflowSmrnaseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params)
         ch_methods_description = Channel.value(methods_description)

From e62e45ce1d029c12e529bd1034255785a899259f Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:11:50 +0000
Subject: [PATCH 200/227] Use paramsSummaryMultiqc function

---
 workflows/smrnaseq.nf | 1 -
 1 file changed, 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index d4e54533..062556c1 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -242,7 +242,6 @@ workflow SMRNASEQ {
     // MODULE: MultiQC
     //
     if (!params.skip_multiqc) {
-        workflow_summary    = WorkflowSmrnaseq.paramsSummaryMultiqc(workflow, summary_params)
         ch_workflow_summary = Channel.value(paramsSummaryMultiqc(workflow_summary))
 
         methods_description    = WorkflowSmrnaseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params)

From ac77ee9ca223f0deb08c9106b122a149b7968c67 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:26:18 +0000
Subject: [PATCH 201/227] Modifications to main workflow and root workflow
 definition

---
 main.nf               | 36 ++++-----------------------
 workflows/smrnaseq.nf | 57 +++++++++++++------------------------------
 2 files changed, 22 insertions(+), 71 deletions(-)

diff --git a/main.nf b/main.nf
index f61907da..24fd7c7c 100644
--- a/main.nf
+++ b/main.nf
@@ -17,10 +17,9 @@ nextflow.enable.dsl = 2
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { SMRNASEQ  } from './workflows/smrnaseq'
+include { NFCORE_SMRNASEQ         } from './workflows/smrnaseq'
 include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_smrnaseq_pipeline'
 include { PIPELINE_COMPLETION     } from './subworkflows/local/utils_nfcore_smrnaseq_pipeline'
-
 include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_smrnaseq_pipeline'
 
 /*
@@ -29,39 +28,10 @@ include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_smrn
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-
 params.fasta            = getGenomeAttribute('fasta')
 params.mirtrace_species = getGenomeAttribute(params, 'mirtrace_species')
 params.bowtie_index     = getGenomeAttribute(params, 'bowtie')
 
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    NAMED WORKFLOWS FOR PIPELINE
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// WORKFLOW: Run main analysis pipeline depending on type of input
-//
-workflow NFCORE_SMRNASEQ {
-
-    take:
-    samplesheet // channel: samplesheet read in from --input
-
-    main:
-
-    //
-    // WORKFLOW: Run pipeline
-    //
-    SMRNASEQ (
-        samplesheet
-    )
-
-    emit:
-    multiqc_report = SMRNASEQ.out.multiqc_report // channel: /path/to/multiqc_report.html
-
-}
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -91,6 +61,9 @@ workflow {
         PIPELINE_INITIALISATION.out.samplesheet
     )
 
+    multiqc_report = NFCORE_SMRNASEQ.out.multiqc_report // channel: /path/to/multiqc_report.html
+
+
     //
     // SUBWORKFLOW: Run completion tasks
     //
@@ -103,6 +76,7 @@ workflow {
         params.hook_url,
         NFCORE_SMRNASEQ.out.multiqc_report
     )
+
 }
 
 /*
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 062556c1..cef7efb4 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -4,9 +4,10 @@
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { MULTIQC                } from '../modules/nf-core/multiqc/main'
-include { paramsSummaryMap       } from 'plugin/nf-validation'
-include { paramsSummaryMultiqc   } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
+
+include { paramsSummaryMap            } from 'plugin/nf-validation'
+include { paramsSummaryMultiqc        } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 
 def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs)
 def citation = '\n' + WorkflowMain.citation(workflow) + '\n'
@@ -29,17 +30,6 @@ ch_multiqc_logo            = params.multiqc_logo   ? Channel.fromPath( params.mu
 ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
 ch_fastp_adapters          = Channel.fromPath(params.fastp_known_mirna_adapters, checkIfExists: true).collect() // collect to consume for all incoming samples to FASTP
 
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT LOCAL MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
-//
-include { INPUT_CHECK } from '../subworkflows/local/input_check'
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
@@ -59,7 +49,7 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-workflow SMRNASEQ {
+workflow NFCORE_SMRNASEQ {
 
     take:
     ch_samplesheet // channel: samplesheet read in from --input
@@ -247,7 +237,7 @@ workflow SMRNASEQ {
         methods_description    = WorkflowSmrnaseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params)
         ch_methods_description = Channel.value(methods_description)
         ch_multiqc_files = Channel.empty()
-        ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
+        ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions )
         ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.collect{it[1]}.ifEmpty([]))
@@ -272,30 +262,17 @@ workflow SMRNASEQ {
     }
 }
 
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    COMPLETION EMAIL AND SUMMARY
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-workflow.onComplete {
-    if (params.email || params.email_on_fail) {
-        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
-    }
-    NfcoreTemplate.dump_parameters(workflow, params)
-    NfcoreTemplate.summary(workflow, params, log)
-    if (params.hook_url) {
-        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
-    }
-}
-
-workflow.onError {
-    if (workflow.errorReport.contains("Process requirement exceeds available memory")) {
-        println("🛑 Default resources exceed availability 🛑 ")
-        println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡")
-    }
-}
+    //
+    // SUBWORKFLOW: Run completion tasks
+    //
+    PIPELINE_COMPLETION (
+        params.email,
+        params.email_on_fail,
+        params.plaintext_email,
+        params.outdir,
+        params.monochrome_logs,
+        params.hook_url
+    )
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From f21a7d524e6f6a8e692a4eb39e5f4549c4fc1fe5 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:27:38 +0000
Subject: [PATCH 202/227] Remove duplicated config entries

---
 nextflow.config | 8 --------
 1 file changed, 8 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 946710ec..34985180 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -55,14 +55,6 @@ params {
     max_cpus                    = 16
     max_time                    = '240.h'
 
-
-    // Schema validation default options
-    validationFailUnrecognisedParams = false
-    validationLenientMode            = false
-    validationSchemaIgnoreParams     = 'genomes'
-    validationShowHiddenParams       = false
-    validate_params                  = true
-
     // Schema validation default options
     validationFailUnrecognisedParams = false
     validationLenientMode            = false

From fcd224e21580a9ff50a9234e6eb4ccdfce178551 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:28:27 +0000
Subject: [PATCH 203/227] Clean up

---
 nextflow.config | 1 -
 1 file changed, 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 34985180..3711e26b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -192,7 +192,6 @@ singularity.registry = 'quay.io'
 
 // Nextflow plugins
 plugins {
-
     id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet
 }
 

From dd24d5fd16fb90aeafbf07d6a108b3667fc43177 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:29:51 +0000
Subject: [PATCH 204/227] Add back config defaults

---
 nextflow.config | 51 +++++++++++++++++++++++++++++++++++++++++++++++++
 1 file changed, 51 insertions(+)

diff --git a/nextflow.config b/nextflow.config
index 3711e26b..34429dde 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -16,6 +16,57 @@ params {
     // Workflow flags
     protocol                    = 'illumina'
 
+    // References
+    genome                      = null
+    igenomes_base               = 's3://ngi-igenomes/igenomes'
+    igenomes_ignore             = false
+    mirna_gtf                   = null
+    mature                      = "https://mirbase.org/download/mature.fa"
+    hairpin                     = "https://mirbase.org/download/hairpin.fa"
+    mirgenedb                   = false
+    mirgenedb_mature            = null
+    mirgenedb_hairpin           = null
+    mirgenedb_gff               = null
+    mirgenedb_species           = null
+    save_aligned                = false
+    save_aligned_mirna_quant    = true
+
+    // UMI handling
+    with_umi                      = false
+    // skips umitools extract in FASTQ_FASTQC_UMITOOLS_FASTP subworkflow. Needs to be true for fastq mode in collapsing reads
+    skip_umi_extract_before_dedup = true
+    umitools_extract_method       = 'string'
+    umitools_bc_pattern           = null
+    umi_discard_read              = null
+    umitools_method               = 'dir'
+    save_umi_intermeds            = false
+
+    // Trimming options
+    clip_r1                     = null
+    three_prime_clip_r1         = null
+    three_prime_adapter         = 'AGATCGGAAGAGCACACGTCTGAACTCCAGTCA'
+    trim_fastq                  = true
+    fastp_min_length            = 17
+    fastp_known_mirna_adapters  = "$projectDir/assets/known_adapters.fa"
+    save_trimmed_fail           = false
+    save_merged                 = true
+    skip_fastqc                 = false
+    skip_multiqc                = false
+    skip_mirdeep                = false
+    skip_fastp                  = false
+    save_reference              = false
+    fastp_max_length            = 100
+    min_trimmed_reads           = 10
+
+    // Contamination filtering
+    filter_contamination        = false
+    rrna                        = null
+    trna                        = null
+    cdna                        = null
+    ncrna                       = null
+    pirna                       = null
+    other_contamination         = null
+
     // References
     genome                     = null
     igenomes_base              = 's3://ngi-igenomes/igenomes/'

From 963f7d46ce4236545fdf9a8ffa3dcfb4c3f63fe7 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:31:29 +0000
Subject: [PATCH 205/227] Clean up

---
 workflows/smrnaseq.nf | 9 ---------
 1 file changed, 9 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index cef7efb4..b2106475 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -9,15 +9,6 @@ include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
 include { paramsSummaryMap            } from 'plugin/nf-validation'
 include { paramsSummaryMultiqc        } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 
-def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs)
-def citation = '\n' + WorkflowMain.citation(workflow) + '\n'
-def summary_params = paramsSummaryMap(workflow)
-
-// Print parameter summary log to screen
-log.info logo + paramsSummaryLog(workflow) + citation
-
-WorkflowSmrnaseq.initialise(params, log)
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     CONFIG FILES

From cfd3e042081d7f0fba09bb20809274b4cafafe77 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:33:06 +0000
Subject: [PATCH 206/227] Moar cleaning

---
 .gitignore            |  1 +
 workflows/smrnaseq.nf | 17 ++---------------
 2 files changed, 3 insertions(+), 15 deletions(-)

diff --git a/.gitignore b/.gitignore
index 5124c9ac..4109b5c9 100644
--- a/.gitignore
+++ b/.gitignore
@@ -6,3 +6,4 @@ results/
 testing/
 testing*
 *.pyc
+execution_trace*
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index b2106475..15470772 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -3,9 +3,9 @@
     IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
-
+include { FASTQC                      } from '../modules/nf-core/fastqc/main'
 include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
-
+include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
 include { paramsSummaryMap            } from 'plugin/nf-validation'
 include { paramsSummaryMultiqc        } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 
@@ -21,19 +21,6 @@ ch_multiqc_logo            = params.multiqc_logo   ? Channel.fromPath( params.mu
 ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
 ch_fastp_adapters          = Channel.fromPath(params.fastp_known_mirna_adapters, checkIfExists: true).collect() // collect to consume for all incoming samples to FASTP
 
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT NF-CORE MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// MODULE: Installed directly from nf-core/modules
-//
-include { FASTQC                      } from '../modules/nf-core/fastqc/main'
-include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
-include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW

From c0e3302a01bd9684f7b4603e5f8e70dd940cd332 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:35:26 +0000
Subject: [PATCH 207/227] Remove old import

---
 workflows/smrnaseq.nf | 1 -
 1 file changed, 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 15470772..96146ba7 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -5,7 +5,6 @@
 */
 include { FASTQC                      } from '../modules/nf-core/fastqc/main'
 include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
-include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
 include { paramsSummaryMap            } from 'plugin/nf-validation'
 include { paramsSummaryMultiqc        } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 

From 3045655a23d86fd4d0d4e8d5d551a63241c8a0da Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:36:13 +0000
Subject: [PATCH 208/227] Cleaning up structure

---
 workflows/smrnaseq.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 96146ba7..3451aa58 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -237,7 +237,6 @@ workflow NFCORE_SMRNASEQ {
         )
         multiqc_report = MULTIQC.out.report.toList()
     }
-}
 
     //
     // SUBWORKFLOW: Run completion tasks
@@ -250,7 +249,7 @@ workflow NFCORE_SMRNASEQ {
         params.monochrome_logs,
         params.hook_url
     )
-
+}
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     THE END

From a72d664abe8c1165d42e3c4694190e7eb008179d Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:56:14 +0000
Subject: [PATCH 209/227] Adhere to new structure

---
 workflows/smrnaseq.nf | 51 +++++++++++--------------------------------
 1 file changed, 13 insertions(+), 38 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 3451aa58..25db71a6 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -29,41 +29,22 @@ ch_fastp_adapters          = Channel.fromPath(params.fastp_known_mirna_adapters,
 workflow NFCORE_SMRNASEQ {
 
     take:
-    ch_samplesheet // channel: samplesheet read in from --input
+    ch_input            // channel: samplesheet file as specified to --input
+    ch_samplesheet      // channel: sample fastqs parsed from --input
+    ch_versions         // channel: [ path(versions.yml) ]
+    ch_fasta            // channel: path(genome.fasta)
 
-    main:
-
-    ch_versions = Channel.empty()
 
-    //
-    // SUBWORKFLOW: Read in samplesheet, validate and stage input files
-    //
-    INPUT_CHECK (
-        file(params.input)
-    )
-    .reads
-    .dump(tag: 'group')
-    .branch {
-        meta, fastq ->
-            single  : fastq.size() == 1
-                return [ meta, fastq.flatten() ]
-            multiple: fastq.size() > 1
-                return [ meta, fastq.flatten() ]
-    }
-    .set { ch_fastq }
-    ch_versions = ch_versions.mix(INPUT_CHECK.out.versions)
-    // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input")
-    // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/
-    // ! There is currently no tooling to help you write a sample sheet schema
+    main:
 
     //
     // MODULE: Concatenate FastQ files from same sample if required
     //
     CAT_FASTQ (
-        ch_fastq.multiple
+        ch_samplesheet.multiple
     )
     .reads
-    .mix(ch_fastq.single)
+    .mix(ch_samplesheet.single)
     .set { ch_cat_fastq }
     ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first().ifEmpty(null))
 
@@ -208,6 +189,7 @@ workflow NFCORE_SMRNASEQ {
     //
     // MODULE: MultiQC
     //
+    ch_multiqc_report = Channel.empty()
     if (!params.skip_multiqc) {
         ch_workflow_summary = Channel.value(paramsSummaryMultiqc(workflow_summary))
 
@@ -235,20 +217,13 @@ workflow NFCORE_SMRNASEQ {
             ch_multiqc_custom_config.toList(),
             ch_multiqc_logo.toList()
         )
-        multiqc_report = MULTIQC.out.report.toList()
+        ch_multiqc_report = MULTIQC.out.report
+
     }
 
-    //
-    // SUBWORKFLOW: Run completion tasks
-    //
-    PIPELINE_COMPLETION (
-        params.email,
-        params.email_on_fail,
-        params.plaintext_email,
-        params.outdir,
-        params.monochrome_logs,
-        params.hook_url
-    )
+    emit:
+    multiqc_report = ch_multiqc_report // channel: /path/to/multiqc_report.html
+    versions       = ch_versions       // channel: [ path(versions.yml) ]
 }
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From db2787d716de5812b186f9e19be70e486d993241 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:56:50 +0000
Subject: [PATCH 210/227] Cleaner

---
 workflows/smrnaseq.nf | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 25db71a6..48e34e4d 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -14,11 +14,11 @@ include { paramsSummaryMultiqc        } from '../subworkflows/nf-core/utils_nfco
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-ch_multiqc_config          = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-ch_multiqc_custom_config   = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty()
-ch_multiqc_logo            = params.multiqc_logo   ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty()
+ch_multiqc_config                     = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+ch_multiqc_custom_config              = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty()
+ch_multiqc_logo                       = params.multiqc_logo   ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty()
 ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-ch_fastp_adapters          = Channel.fromPath(params.fastp_known_mirna_adapters, checkIfExists: true).collect() // collect to consume for all incoming samples to FASTP
+ch_fastp_adapters                     = Channel.fromPath(params.fastp_known_mirna_adapters, checkIfExists: true).collect() // collect to consume for all incoming samples to FASTP
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From 60de7cac48200cec6b4cac6fb83a6437895ce569 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 13:59:52 +0000
Subject: [PATCH 211/227] Follow the lead

---
 main.nf               | 12 ++++++------
 workflows/smrnaseq.nf |  2 --
 2 files changed, 6 insertions(+), 8 deletions(-)

diff --git a/main.nf b/main.nf
index 24fd7c7c..cd13268a 100644
--- a/main.nf
+++ b/main.nf
@@ -29,8 +29,8 @@ include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_smrn
 */
 
 params.fasta            = getGenomeAttribute('fasta')
-params.mirtrace_species = getGenomeAttribute(params, 'mirtrace_species')
-params.bowtie_index     = getGenomeAttribute(params, 'bowtie')
+params.mirtrace_species = getGenomeAttribute('mirtrace_species')
+params.bowtie_index     = getGenomeAttribute('bowtie')
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -40,6 +40,7 @@ params.bowtie_index     = getGenomeAttribute(params, 'bowtie')
 workflow {
 
     main:
+    ch_versions = Channel.empty()
 
     //
     // SUBWORKFLOW: Run initialisation tasks
@@ -58,12 +59,11 @@ workflow {
     // WORKFLOW: Run main workflow
     //
     NFCORE_SMRNASEQ (
-        PIPELINE_INITIALISATION.out.samplesheet
+        Channel.of(file(params.input, checkIfExists: true)),
+        PIPELINE_INITIALISATION.out.samplesheet,
+        ch_versions
     )
 
-    multiqc_report = NFCORE_SMRNASEQ.out.multiqc_report // channel: /path/to/multiqc_report.html
-
-
     //
     // SUBWORKFLOW: Run completion tasks
     //
diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 48e34e4d..ecf07b99 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -32,8 +32,6 @@ workflow NFCORE_SMRNASEQ {
     ch_input            // channel: samplesheet file as specified to --input
     ch_samplesheet      // channel: sample fastqs parsed from --input
     ch_versions         // channel: [ path(versions.yml) ]
-    ch_fasta            // channel: path(genome.fasta)
-
 
     main:
 

From f5044d2114849b2112ee3bec9ebfd8b29e3a416c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 14:06:36 +0000
Subject: [PATCH 212/227] Clean up imports

---
 workflows/smrnaseq.nf | 37 +++++++++++++++++++++++++++++++------
 1 file changed, 31 insertions(+), 6 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index ecf07b99..6b359c69 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -3,10 +3,22 @@
     IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
-include { FASTQC                      } from '../modules/nf-core/fastqc/main'
-include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
-include { paramsSummaryMap            } from 'plugin/nf-validation'
-include { paramsSummaryMultiqc        } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { FASTQ_FASTQC_UMITOOLS_FASTP      } from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp'
+include { FASTP as FASTP_LENGTH_FILTER     } from '../modules/nf-core/fastp'
+include { CONTAMINANT_FILTER               } from '../subworkflows/local/contaminant_filter'
+include { MIRNA_QUANT                      } from '../subworkflows/local/mirna_quant'
+include { GENOME_QUANT                     } from '../subworkflows/local/genome_quant'
+include { MIRDEEP2                         } from '../subworkflows/local/mirdeep2'
+include { INDEX_GENOME                     } from '../modules/local/bowtie_genome'
+include { MIRTRACE                         } from '../subworkflows/local/mirtrace'
+include { CAT_FASTQ                        } from '../modules/nf-core/cat/fastq/main'
+include { MULTIQC                          } from '../modules/nf-core/multiqc/main'
+include { UMICOLLAPSE as UMICOLLAPSE_FASTQ } from '../modules/nf-core/umicollapse/main'
+include { UMITOOLS_EXTRACT                 } from '../modules/nf-core/umitools/extract/main'
+include { FASTQC                           } from '../modules/nf-core/fastqc/main'
+include { MULTIQC                          } from '../modules/nf-core/multiqc/main'
+include { paramsSummaryMap                 } from 'plugin/nf-validation'
+include { paramsSummaryMultiqc             } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -36,14 +48,27 @@ workflow NFCORE_SMRNASEQ {
     main:
 
     //
+    // Create separate channels for samples that have single/multiple FastQ files to merge
+    //
+    ch_samplesheet
+        .branch {
+            meta, fastqs ->
+                single  : fastqs.size() == 1
+                    return [ meta, fastqs.flatten() ]
+                multiple: fastqs.size() > 1
+                    return [ meta, fastqs.flatten() ]
+        }
+        .set { ch_fastq }
+
     // MODULE: Concatenate FastQ files from same sample if required
     //
     CAT_FASTQ (
-        ch_samplesheet.multiple
+        ch_fastq.multiple
     )
     .reads
-    .mix(ch_samplesheet.single)
+    .mix(ch_fastq.single)
     .set { ch_cat_fastq }
+
     ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first().ifEmpty(null))
 
     mirna_adapters = params.with_umi ? [] : params.fastp_known_mirna_adapters

From 62304479a5d9db739b37f2da340e3adef3cffb7a Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 14:07:54 +0000
Subject: [PATCH 213/227] Order!

---
 workflows/smrnaseq.nf | 12 ++++++------
 1 file changed, 6 insertions(+), 6 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 6b359c69..9a204687 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -3,20 +3,20 @@
     IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
+include { CAT_FASTQ                        } from '../modules/nf-core/cat/fastq/main'
+include { CONTAMINANT_FILTER               } from '../subworkflows/local/contaminant_filter'
+include { FASTQC                           } from '../modules/nf-core/fastqc/main'
 include { FASTQ_FASTQC_UMITOOLS_FASTP      } from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp'
 include { FASTP as FASTP_LENGTH_FILTER     } from '../modules/nf-core/fastp'
-include { CONTAMINANT_FILTER               } from '../subworkflows/local/contaminant_filter'
-include { MIRNA_QUANT                      } from '../subworkflows/local/mirna_quant'
 include { GENOME_QUANT                     } from '../subworkflows/local/genome_quant'
-include { MIRDEEP2                         } from '../subworkflows/local/mirdeep2'
 include { INDEX_GENOME                     } from '../modules/local/bowtie_genome'
+include { MIRNA_QUANT                      } from '../subworkflows/local/mirna_quant'
+include { MIRDEEP2                         } from '../subworkflows/local/mirdeep2'
 include { MIRTRACE                         } from '../subworkflows/local/mirtrace'
-include { CAT_FASTQ                        } from '../modules/nf-core/cat/fastq/main'
 include { MULTIQC                          } from '../modules/nf-core/multiqc/main'
 include { UMICOLLAPSE as UMICOLLAPSE_FASTQ } from '../modules/nf-core/umicollapse/main'
 include { UMITOOLS_EXTRACT                 } from '../modules/nf-core/umitools/extract/main'
-include { FASTQC                           } from '../modules/nf-core/fastqc/main'
-include { MULTIQC                          } from '../modules/nf-core/multiqc/main'
+
 include { paramsSummaryMap                 } from 'plugin/nf-validation'
 include { paramsSummaryMultiqc             } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 

From a208112deb3703bd8412dae51b9f96f4dbd32c78 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 14:10:00 +0000
Subject: [PATCH 214/227] Add checks back in

---
 workflows/smrnaseq.nf | 11 ++++++++++-
 1 file changed, 10 insertions(+), 1 deletion(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 9a204687..35c9e272 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -46,7 +46,16 @@ workflow NFCORE_SMRNASEQ {
     ch_versions         // channel: [ path(versions.yml) ]
 
     main:
-
+    //Config checks
+    if (!params.mirgenedb) {
+    if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature miRNA fasta file not found: ${params.mature}" }
+    if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin miRNA fasta file not found: ${params.hairpin}" }
+    } else {
+    if (params.mirgenedb_mature) { reference_mature = file(params.mirgenedb_mature, checkIfExists: true) } else { exit 1, "Mature miRNA fasta file not found: ${params.mirgenedb_mature}" }
+    if (params.mirgenedb_hairpin) { reference_hairpin = file(params.mirgenedb_hairpin, checkIfExists: true) } else { exit 1, "Hairpin miRNA fasta file not found: ${params.mirgenedb_hairpin}" }
+    if (params.mirgenedb_gff) { mirna_gtf = file(params.mirgenedb_gff, checkIfExists: true) } else { exit 1, "MirGeneDB gff file not found: ${params.mirgenedb_gff}"}
+    if (!params.mirgenedb_species) { exit 1, "MirGeneDB species not set, please specify via the --mirgenedb_species parameter"}
+    }
     //
     // Create separate channels for samples that have single/multiple FastQ files to merge
     //

From e1778fa2dca3d4f01c30c7e906fd6a676caa2881 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 14:21:06 +0000
Subject: [PATCH 215/227] Guess who's back, back again? YAMLs back, call a
 friend.

---
 workflows/smrnaseq.nf | 28 ++++++++++++++++++----------
 1 file changed, 18 insertions(+), 10 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 35c9e272..48239224 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -19,6 +19,7 @@ include { UMITOOLS_EXTRACT                 } from '../modules/nf-core/umitools/e
 
 include { paramsSummaryMap                 } from 'plugin/nf-validation'
 include { paramsSummaryMultiqc             } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { softwareVersionsToYAML           } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -29,7 +30,6 @@ include { paramsSummaryMultiqc             } from '../subworkflows/nf-core/utils
 ch_multiqc_config                     = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
 ch_multiqc_custom_config              = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty()
 ch_multiqc_logo                       = params.multiqc_logo   ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty()
-ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
 ch_fastp_adapters                     = Channel.fromPath(params.fastp_known_mirna_adapters, checkIfExists: true).collect() // collect to consume for all incoming samples to FASTP
 
 /*
@@ -47,14 +47,23 @@ workflow NFCORE_SMRNASEQ {
 
     main:
     //Config checks
+    // Check optional parameters
+    if (!params.mirtrace_species) {
+            exit 1, "Reference species for miRTrace is not defined via the --mirtrace_species parameter."
+        }
+
+    // Genome options
+    def mirna_gtf_from_species = params.mirtrace_species ? "https://mirbase.org/download/CURRENT/genomes/${params.mirtrace_species}.gff3" : false
+    def mirna_gtf = params.mirna_gtf ?: mirna_gtf_from_species
+
     if (!params.mirgenedb) {
-    if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature miRNA fasta file not found: ${params.mature}" }
-    if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin miRNA fasta file not found: ${params.hairpin}" }
+        if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature miRNA fasta file not found: ${params.mature}" }
+        if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin miRNA fasta file not found: ${params.hairpin}" }
     } else {
-    if (params.mirgenedb_mature) { reference_mature = file(params.mirgenedb_mature, checkIfExists: true) } else { exit 1, "Mature miRNA fasta file not found: ${params.mirgenedb_mature}" }
-    if (params.mirgenedb_hairpin) { reference_hairpin = file(params.mirgenedb_hairpin, checkIfExists: true) } else { exit 1, "Hairpin miRNA fasta file not found: ${params.mirgenedb_hairpin}" }
-    if (params.mirgenedb_gff) { mirna_gtf = file(params.mirgenedb_gff, checkIfExists: true) } else { exit 1, "MirGeneDB gff file not found: ${params.mirgenedb_gff}"}
-    if (!params.mirgenedb_species) { exit 1, "MirGeneDB species not set, please specify via the --mirgenedb_species parameter"}
+        if (params.mirgenedb_mature) { reference_mature = file(params.mirgenedb_mature, checkIfExists: true) } else { exit 1, "Mature miRNA fasta file not found: ${params.mirgenedb_mature}" }
+        if (params.mirgenedb_hairpin) { reference_hairpin = file(params.mirgenedb_hairpin, checkIfExists: true) } else { exit 1, "Hairpin miRNA fasta file not found: ${params.mirgenedb_hairpin}" }
+        if (params.mirgenedb_gff) { mirna_gtf = file(params.mirgenedb_gff, checkIfExists: true) } else { exit 1, "MirGeneDB gff file not found: ${params.mirgenedb_gff}"}
+        if (!params.mirgenedb_species) { exit 1, "MirGeneDB species not set, please specify via the --mirgenedb_species parameter"}
     }
     //
     // Create separate channels for samples that have single/multiple FastQ files to merge
@@ -223,10 +232,9 @@ workflow NFCORE_SMRNASEQ {
     //
     ch_multiqc_report = Channel.empty()
     if (!params.skip_multiqc) {
-        ch_workflow_summary = Channel.value(paramsSummaryMultiqc(workflow_summary))
+        summary_params           = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
+        ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
 
-        methods_description    = WorkflowSmrnaseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params)
-        ch_methods_description = Channel.value(methods_description)
         ch_multiqc_files = Channel.empty()
         ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions )
         ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))

From e78276741964da18e02c58b52acd33301a4d30e6 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 14:24:21 +0000
Subject: [PATCH 216/227] Adjust modules config to new structure

---
 conf/modules.config | 30 +++++++++++++++---------------
 1 file changed, 15 insertions(+), 15 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index fb26908e..f7a50622 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -26,7 +26,7 @@ process {
     //
     // Input preparation
     //
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK' {
+    withName: 'NFCORE_SMRNASEQ:INPUT_CHECK:SAMPLESHEET_CHECK' {
         publishDir = [
             path: { "${params.outdir}/pipeline_info" },
             mode: params.publish_dir_mode,
@@ -187,7 +187,7 @@ process {
     //
     // CONTAMINANT_FILTER
     //
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:CONTAMINANT_FILTER:.*' {
+    withName: 'NFCORE_SMRNASEQ:CONTAMINANT_FILTER:.*' {
         publishDir = [
             path: { "${params.outdir}/contaminant_filter/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
             mode: params.publish_dir_mode,
@@ -198,35 +198,35 @@ process {
     //
     // MIRNA_QUANT
     //
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:FORMAT_.*' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:FORMAT_.*' {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/reference" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:PARSE_.*' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:PARSE_.*' {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/reference" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:INDEX_MATURE' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:INDEX_MATURE' {
         publishDir = [
             path: { "${params.outdir}/bowtie_index/mirna_mature" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:INDEX_HAIRPIN' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:INDEX_HAIRPIN' {
         publishDir = [
             path: { "${params.outdir}/bowtie_index/mirna_hairpin" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_MATURE' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_MATURE' {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/mature" },
             mode: params.publish_dir_mode,
@@ -234,7 +234,7 @@ process {
             enabled: params.save_aligned_mirna_quant
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BAM_STATS_MATURE:.*' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:BAM_STATS_MATURE:.*' {
         ext.prefix = { "${meta.id}.sorted" }
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/mature" },
@@ -242,7 +242,7 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_HAIRPIN' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_HAIRPIN' {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/hairpin" },
             mode: params.publish_dir_mode,
@@ -250,7 +250,7 @@ process {
             enabled: params.save_aligned_mirna_quant
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BAM_STATS_HAIRPIN:.*' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:BAM_STATS_HAIRPIN:.*' {
         ext.prefix = { "${meta.id}.sorted" }
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/hairpin" },
@@ -272,7 +272,7 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_SEQCLUSTER' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:BOWTIE_MAP_SEQCLUSTER' {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/bam/seqcluster" },
             mode: params.publish_dir_mode,
@@ -288,7 +288,7 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:MIRNA_QUANT:TABLE_MERGE' {
+    withName: 'NFCORE_SMRNASEQ:MIRNA_QUANT:TABLE_MERGE' {
         publishDir = [
             path: { "${params.outdir}/mirna_quant/mirtop" },
             mode: params.publish_dir_mode,
@@ -300,7 +300,7 @@ process {
     //
     // GENOME_QUANT
     //
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_.*' {
+    withName: 'NFCORE_SMRNASEQ:GENOME_QUANT:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_.*' {
         ext.prefix = { "${meta.id}.sorted" }
         publishDir = [
             path: { "${params.outdir}/genome_quant/bam" },
@@ -308,7 +308,7 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:.*' {
+    withName: 'NFCORE_SMRNASEQ:GENOME_QUANT:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:.*' {
         ext.prefix = { "${meta.id}.sorted" }
         publishDir = [
             path: { "${params.outdir}/genome_quant/bam" },
@@ -316,7 +316,7 @@ process {
             pattern: "*.{stats,flagstat,idxstats}"
         ]
     }
-    withName: 'NFCORE_SMRNASEQ:SMRNASEQ:GENOME_QUANT:BOWTIE_MAP_GENOME' {
+    withName: 'NFCORE_SMRNASEQ:GENOME_QUANT:BOWTIE_MAP_GENOME' {
         publishDir = [
             path: { "${params.outdir}/genome_quant/bam" },
             mode: params.publish_dir_mode,

From ff34b0ff9fa47cf7854c10486df309e23690e010 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Wed, 21 Feb 2024 14:26:53 +0000
Subject: [PATCH 217/227] Add default bowtie_index = null

---
 nextflow.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/nextflow.config b/nextflow.config
index 34429dde..87fda757 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -30,6 +30,7 @@ params {
     mirgenedb_species           = null
     save_aligned                = false
     save_aligned_mirna_quant    = true
+    bowtie_index                = null
 
     // UMI handling
     with_umi                      = false

From 7bd08bd8dd991e014acdc06afbec99e31c2d8648 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 22 Feb 2024 14:40:15 +0000
Subject: [PATCH 218/227] Added back softwareVresionsYaml

---
 workflows/smrnaseq.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index 9e3ff10e..e03f90e1 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -230,8 +230,8 @@ workflow NFCORE_SMRNASEQ {
     // Collate and save software versions
     //
     softwareVersionsToYAML(ch_versions)
-        .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true)
-        .set { ch_collated_versions }
+        .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_smrnaseq_software_mqc_versions.yml', sort: true, newLine: true)
+        .set {ch_collated_versions}
 
     //
     // MODULE: MultiQC

From c5e83930f603cddfe3fa3e009a75d500f560809c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 22 Feb 2024 15:06:42 +0000
Subject: [PATCH 219/227] Fix thanks to arthur and mahesh

---
 workflows/smrnaseq.nf | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/workflows/smrnaseq.nf b/workflows/smrnaseq.nf
index e03f90e1..9252fec0 100644
--- a/workflows/smrnaseq.nf
+++ b/workflows/smrnaseq.nf
@@ -87,7 +87,7 @@ workflow NFCORE_SMRNASEQ {
     .mix(ch_fastq.single)
     .set { ch_cat_fastq }
 
-    ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first().ifEmpty(null))
+    ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first())
 
     mirna_adapters = params.with_umi ? [] : params.fastp_known_mirna_adapters
 
@@ -170,7 +170,7 @@ workflow NFCORE_SMRNASEQ {
     // SUBWORKFLOW: MIRTRACE
     //
     MIRTRACE(ch_mirtrace_inputs)
-    ch_versions = ch_versions.mix(MIRTRACE.out.versions.ifEmpty(null))
+    ch_versions = ch_versions.mix(MIRTRACE.out.versions)
 
     //
     // SUBWORKFLOW: remove contaminants from reads
@@ -200,7 +200,7 @@ workflow NFCORE_SMRNASEQ {
         mirna_gtf,
         ch_reads_for_mirna
     )
-    ch_versions = ch_versions.mix(MIRNA_QUANT.out.versions.ifEmpty(null))
+    ch_versions = ch_versions.mix(MIRNA_QUANT.out.versions)
 
     //
     // GENOME
@@ -242,7 +242,7 @@ workflow NFCORE_SMRNASEQ {
         ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
 
         ch_multiqc_files = Channel.empty()
-        ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions )
+        ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
         ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.collect{it[1]}.ifEmpty([]))

From 761b71c2a18b0658f48af0b59c2a892232742cda Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 22 Feb 2024 19:59:00 +0000
Subject: [PATCH 220/227] Add proper changelog updates

---
 CHANGELOG.md | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3eb46248..fa3194ac 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.3.0 - 2024-02-09 - Gray Zinc Dalmatian
+## v2.3.0 - 2024-02-22 - Gray Zinc Dalmatian
 
 - [[#307]](https://github.com/nf-core/smrnaseq/pull/307) - Clean up config file and improve output folder structure
 - [[#299]](https://github.com/nf-core/smrnaseq/issues/299) - Bugfix for missing inputs in BAM stats (`genome_quant.r`)
@@ -14,6 +14,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [[#310]](https://github.com/nf-core/smrnaseq/pull/310) - Removed unnecessarily separate mirtrace subworkflow, now using module instead
 - [[#311]](https://github.com/nf-core/smrnaseq/pull/311) - Fix use of FASTP, set `three_prime_adapter` per default
 - [[#314]](https://github.com/nf-core/smrnaseq/pull/314) - Add parameters to control publishing of intermediate results
+- [[#317]](https://github.com/nf-core/smrnaseq/pull/317) - Fixed issue with bowtie indices directly supplied
+- [[#318]](https://github.com/nf-core/smrnaseq/pull/318) - Merged in nf-core template v2.13.0 and pinned nf-validator
 
 ### Parameters
 

From f7f63316cc2fc7c99c7975ba4ba64ec1331c2ebe Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 22 Feb 2024 20:05:27 +0000
Subject: [PATCH 221/227] Add proper changelog updates

---
 CHANGELOG.md                                  |  2 +-
 modules.json                                  | 44 ++-----------------
 modules/nf-core/cat/fastq/environment.yml     |  2 +-
 modules/nf-core/cat/fastq/main.nf             |  1 -
 .../umicollapse/tests/main.nf.test.snap       | 24 ++++++++--
 .../nf-core/umitools/extract/environment.yml  |  2 +-
 modules/nf-core/umitools/extract/main.nf      |  4 +-
 .../umitools/extract/tests/main.nf.test.snap  |  8 +++-
 8 files changed, 34 insertions(+), 53 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index fa3194ac..33f55609 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -44,7 +44,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | `seqkit`      | 2.3.1       | 2.6.1       |
 | `fastqc`      | 0.11.4      | 0.12.1      |
 | `samtools`    | 1.17        | 1.19.2      |
-| `umitools`    | <none>      | 1.1.4       |
+| `umitools`    | <none>      | 1.1.5       |
 | `umicollapse` | <none>      | 1.0.0       |
 
 ## [v2.2.4](https://github.com/nf-core/smrnaseq/releases/tag/2.2.4) - 2023-11-03
diff --git a/modules.json b/modules.json
index ce269067..9bcc37e7 100644
--- a/modules.json
+++ b/modules.json
@@ -12,12 +12,7 @@
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "02fd5bd7275abad27aad32d5c852e0a9b1b98882",
-                        "installed_by": ["modules"]
-                    },
-                    "custom/dumpsoftwareversions": {
-                        "branch": "master",
-                        "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1",
+                        "git_sha": "0997b47c93c06b49aa7b3fefda87e728312cf2ca",
                         "installed_by": ["modules"]
                     },
                     "fastp": {
@@ -62,12 +57,12 @@
                     },
                     "umicollapse": {
                         "branch": "master",
-                        "git_sha": "2e561f4551ce3df2bc60016f7ea359f2ed4bce49",
+                        "git_sha": "ba3f3df395d2719dcef5c67189042a1dc555c701",
                         "installed_by": ["modules"]
                     },
                     "umitools/extract": {
                         "branch": "master",
-                        "git_sha": "9e56d7a647fbf6f7e45ef123bc916ad66b6f7c9d",
+                        "git_sha": "ff7e93715a2acecf3f143ec78c9858deba2984d0",
                         "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "untarfiles": {
@@ -79,44 +74,11 @@
             },
             "subworkflows": {
                 "nf-core": {
-                    "bam_sort_stats_samtools": {
-                        "branch": "master",
-                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
-                        "installed_by": ["subworkflows"]
-                    },
-                    "bam_stats_samtools": {
-                        "branch": "master",
-                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
-                        "installed_by": ["bam_sort_stats_samtools", "subworkflows"]
-                    },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
                         "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56",
                         "installed_by": ["subworkflows"]
-                    }
-                }
-            },
-            "subworkflows": {
-                "nf-core": {
-                    "utils_nextflow_pipeline": {
-                        "branch": "master",
-                        "git_sha": "cd08c91373cd00a73255081340e4914485846ba1",
-                        "installed_by": ["subworkflows"]
-                    },
-                    "utils_nfcore_pipeline": {
-                        "branch": "master",
-                        "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8",
-                        "installed_by": ["subworkflows"]
                     },
-                    "utils_nfvalidation_plugin": {
-                        "branch": "master",
-                        "git_sha": "cd08c91373cd00a73255081340e4914485846ba1",
-                        "installed_by": ["subworkflows"]
-                    }
-                }
-            },
-            "subworkflows": {
-                "nf-core": {
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "cd08c91373cd00a73255081340e4914485846ba1",
diff --git a/modules/nf-core/cat/fastq/environment.yml b/modules/nf-core/cat/fastq/environment.yml
index bff93add..8c69b121 100644
--- a/modules/nf-core/cat/fastq/environment.yml
+++ b/modules/nf-core/cat/fastq/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - conda-forge::sed=4.7
+  - conda-forge::coreutils=8.30
diff --git a/modules/nf-core/cat/fastq/main.nf b/modules/nf-core/cat/fastq/main.nf
index 3d963784..f132b2ad 100644
--- a/modules/nf-core/cat/fastq/main.nf
+++ b/modules/nf-core/cat/fastq/main.nf
@@ -76,5 +76,4 @@ process CAT_FASTQ {
             """
         }
     }
-
 }
diff --git a/modules/nf-core/umicollapse/tests/main.nf.test.snap b/modules/nf-core/umicollapse/tests/main.nf.test.snap
index 900ff08f..23916f0b 100644
--- a/modules/nf-core/umicollapse/tests/main.nf.test.snap
+++ b/modules/nf-core/umicollapse/tests/main.nf.test.snap
@@ -7,14 +7,18 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.dedup.bam:md5,4e86d60aa82242889ab5f9031418ab2e"
+                    "test.dedup.bam:md5,05c5331185263cbee6f508c0669be864"
                 ]
             ],
             [
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
-        "timestamp": "2024-01-30T10:45:28.352669757"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-19T11:24:31.850566925"
     },
     "umicollapse fastq tests": {
         "content": [
@@ -31,6 +35,10 @@
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-30T10:45:56.053352008"
     },
     "umicollapse stub tests": {
@@ -86,6 +94,10 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2024-01-30T10:46:12.482697713"
     },
     "umicollapse paired tests": {
@@ -96,13 +108,17 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.dedup.bam:md5,54be836ec246073e60212445b4369a91"
+                    "test.dedup.bam:md5,f4f05467cb456309fe22851d8b4d4387"
                 ]
             ],
             [
                 "versions.yml:md5,c1e0275d81b1c97a9344d216f9154996"
             ]
         ],
-        "timestamp": "2024-01-30T10:45:44.961261065"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-19T11:24:44.166029769"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/umitools/extract/environment.yml b/modules/nf-core/umitools/extract/environment.yml
index 7d08ac0e..aab452d1 100644
--- a/modules/nf-core/umitools/extract/environment.yml
+++ b/modules/nf-core/umitools/extract/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::umi_tools=1.1.4
+  - bioconda::umi_tools=1.1.5
diff --git a/modules/nf-core/umitools/extract/main.nf b/modules/nf-core/umitools/extract/main.nf
index 4bd79e79..8719e5f6 100644
--- a/modules/nf-core/umitools/extract/main.nf
+++ b/modules/nf-core/umitools/extract/main.nf
@@ -5,8 +5,8 @@ process UMITOOLS_EXTRACT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.4--py38hbff2b2d_1' :
-        'biocontainers/umi_tools:1.1.4--py38hbff2b2d_1' }"
+        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.5--py39hf95cd2a_0' :
+        'biocontainers/umi_tools:1.1.5--py39hf95cd2a_0' }"
 
     input:
     tuple val(meta), path(reads)
diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test.snap b/modules/nf-core/umitools/extract/tests/main.nf.test.snap
index 6d5944f1..bf82701d 100644
--- a/modules/nf-core/umitools/extract/tests/main.nf.test.snap
+++ b/modules/nf-core/umitools/extract/tests/main.nf.test.snap
@@ -2,9 +2,13 @@
     "versions": {
         "content": [
             [
-                "versions.yml:md5,5a18da2d3a5a4de15e7aaae9082d7abb"
+                "versions.yml:md5,568d243174c081a0301e74ed42e59b48"
             ]
         ],
-        "timestamp": "2023-12-08T09:41:43.540658352"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-16T10:01:33.326046137"
     }
 }
\ No newline at end of file

From 734ce7f6f73cbd99cf5d486c791f67806507336e Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 22 Feb 2024 20:07:10 +0000
Subject: [PATCH 222/227] Fix image

---
 docs/images/nf-core-smrnaseq_logo_light.png | Bin 25106 -> 25078 bytes
 1 file changed, 0 insertions(+), 0 deletions(-)

diff --git a/docs/images/nf-core-smrnaseq_logo_light.png b/docs/images/nf-core-smrnaseq_logo_light.png
index 62d5d1d71bee7b1d87536f0f06f6a5c2d8375eb2..8ec03f57cad78a3c58be11b86d954ee972513d22 100644
GIT binary patch
delta 23906
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From 9193a5cb0fb0f9a2e6c70165cdc52ea1b6f873c1 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 22 Feb 2024 20:17:08 +0000
Subject: [PATCH 223/227] Fix modules

---
 modules.json | 10 ++++++++++
 1 file changed, 10 insertions(+)

diff --git a/modules.json b/modules.json
index 9bcc37e7..ad13815e 100644
--- a/modules.json
+++ b/modules.json
@@ -74,6 +74,16 @@
             },
             "subworkflows": {
                 "nf-core": {
+                    "bam_sort_stats_samtools": {
+                        "branch": "master",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
+                        "installed_by": ["subworkflows"]
+                    },
+                    "bam_stats_samtools": {
+                        "branch": "master",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
+                        "installed_by": ["bam_sort_stats_samtools"]
+                    },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
                         "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56",

From b041d83f271966568809cafbb40c5c46a4f16859 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 22 Feb 2024 20:19:46 +0000
Subject: [PATCH 224/227] Fix lint

---
 docs/images/nf-core-smrnaseq_logo_light.png | Bin 25078 -> 25106 bytes
 1 file changed, 0 insertions(+), 0 deletions(-)

diff --git a/docs/images/nf-core-smrnaseq_logo_light.png b/docs/images/nf-core-smrnaseq_logo_light.png
index 8ec03f57cad78a3c58be11b86d954ee972513d22..62d5d1d71bee7b1d87536f0f06f6a5c2d8375eb2 100644
GIT binary patch
delta 23952
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From f923057db18f6330a6ea1442e1bae98039cbba16 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <apeltzer@users.noreply.github.com>
Date: Fri, 23 Feb 2024 09:28:56 +0100
Subject: [PATCH 225/227] Update CHANGELOG.md

Co-authored-by: Christopher Mohr <contact.cmohr@gmail.com>
---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 33f55609..efe49ef2 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.3.0 - 2024-02-22 - Gray Zinc Dalmatian
+## v2.3.0 - 2024-02-23 - Gray Zinc Dalmatian
 
 - [[#307]](https://github.com/nf-core/smrnaseq/pull/307) - Clean up config file and improve output folder structure
 - [[#299]](https://github.com/nf-core/smrnaseq/issues/299) - Bugfix for missing inputs in BAM stats (`genome_quant.r`)

From 472a6472447d1e02b8b6d9b42a52d6f4d6b59e94 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 23 Feb 2024 09:05:45 +0000
Subject: [PATCH 226/227] Add back changelog stuf

---
 CHANGELOG.md | 31 ++++++++++++++++++++++++++++---
 1 file changed, 28 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 33f55609..2a00468c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -54,6 +54,23 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [[#288]](https://github.com/nf-core/smrnaseq/issues/288) - Bugfix for issue with handling malformed GFF3 from mirbase
 - Updated dependencies, including FASTQC, MultiQC 1.17, fastP and samtools to latest versions
 
+### Parameters
+
+| Old parameter | New parameter            |
+| ------------- | ------------------------ |
+|               | `--mirgenedb`            |
+|               | `--mirgenedb_species`    |
+|               | `--mirgenedb_gff`        |
+|               | `--mirgenedb_mature`     |
+|               | `--mirgenedb_hairpin`    |
+|               | `--contamination_filter` |
+|               | `--rrna`                 |
+|               | `--trna`                 |
+|               | `--cdna`                 |
+|               | `--ncrna`                |
+|               | `--pirna`                |
+|               | `--other_contamination`  |
+
 ## [v2.2.3](https://github.com/nf-core/smrnaseq/releases/tag/2.2.3) - 2023-09-06
 
 - [[#271]](https://github.com/nf-core/smrnaseq/issues/271) - Bugfix for parsing hairpin and mature fasta files
@@ -153,9 +170,17 @@ Note, since the pipeline is now using Nextflow DSL2, each process will be run wi
 | `bowtie2`            | -           | 2.4.5       |
 | `blat`               | -           | 36          |
 
-> **NB:** Dependency has been **updated** if both old and new version information is present.
-> **NB:** Dependency has been **added** if just the new version information is present.
-> **NB:** Dependency has been **removed** if version information isn't present.
+### Parameters
+
+| Old parameter        | New parameter    |
+| -------------------- | ---------------- |
+| `--conda`            | `--enable_conda` |
+| `--clusterOptions`   |                  |
+| `--publish_dir_mode` |                  |
+
+> **NB:** Parameter has been **updated** if both old and new parameter information is present.
+> **NB:** Parameter has been **added** if just the new parameter information is present.
+> **NB:** Parameter has been **removed** if parameter information isn't present.
 
 ## [v1.1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.1.0) - 2021-06-15
 

From 969e3357ec389664ce500a9724413a12100f7c1c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Fri, 23 Feb 2024 09:40:51 +0000
Subject: [PATCH 227/227] Remove todo

---
 .github/workflows/awsfulltest.yml | 1 -
 1 file changed, 1 deletion(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 3c8cf6d6..1795a0fb 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -15,7 +15,6 @@ jobs:
     steps:
       - name: Launch workflow via tower
         uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2
-        # TODO nf-core: You can customise AWS full pipeline tests as required
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
         with: