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When running scrnaseq v 2.4.0 with the new cellranger #246 , we are encountering errors like this. This happens on all samples starting with SRR1xxx.
AssertionError: We expect R1 and R2 of the same sample to have the same filename except for R1/R2.
Files involved:
- fastq_001/SRR14923965_S1_L001_R1_001.fastq.gz
- fastq_002/SRR14923965_S1_L001_R2_001.fastq.gz
A look at the command indicates the error is due to how the renaming of r1.name for the comparison for r2.name, in particular: if r1.name.replace("R1", "R2") != r2.name:
Instead, this line should read: if r1.name.replace("_R1_", "_R2_") != r2.name: to ensure sample IDs containing "R1" in them don't get renamed by mistake.
Command used and terminal output
No response
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered:
Description of the bug
When running scrnaseq v 2.4.0 with the new cellranger #246 , we are encountering errors like this. This happens on all samples starting with SRR1xxx.
A look at the command indicates the error is due to how the renaming of r1.name for the comparison for r2.name, in particular:
if r1.name.replace("R1", "R2") != r2.name:
Instead, this line should read:
if r1.name.replace("_R1_", "_R2_") != r2.name:
to ensure sample IDs containing "R1" in them don't get renamed by mistake.Command used and terminal output
No response
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered: