From 758fd35e720eb8c49b29ae5e98fe6ef55d4806d6 Mon Sep 17 00:00:00 2001
From: brucemoran <bruce.moran@gmail.com>
Date: Thu, 23 Jul 2020 13:40:59 +0100
Subject: [PATCH 001/359] GRCh37 option

---
 CHANGELOG.md           |  3 +-
 conf/genomes.config    | 12 ++++++-
 docs/references.md     | 11 ++++++
 docs/usage.md          |  4 +--
 download-references.nf | 78 +++++++++++++++++++++++++++---------------
 main.nf                | 35 +++++++++++--------
 6 files changed, 96 insertions(+), 47 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5a990f62..a2306ebe 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -5,11 +5,12 @@ All notable changes to this project will be documented in this file.
 The format is based on [Keep a Changelog](http://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0.html).
 
-## [1.2.0] nfcore/rnafusion - 2020/07/15
+## dev nfcore/rnafusion - 2020/07/15
 
 ### Added
 
 * Added social preview image [#107](https://github.com/nf-core/rnafusion/issues/107)
+* Added support for GRCh37 genome assembly [#77](https://github.com/nf-core/rnafusion/issues/77)
 
 ### Changed
 
diff --git a/conf/genomes.config b/conf/genomes.config
index 985345cc..934041b2 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -19,5 +19,15 @@ params {
             fusioncatcher_ref   = "${params.genomes_base}/fusioncatcher/human_v98"
             star_fusion_ref     = "${params.genomes_base}/star-fusion/ctat_genome_lib_build_dir"
         }
+        'GRCh37' {
+            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.all.fa"
+            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.gtf"
+            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.cdna.all.fa.gz"
+            databases           = "${params.genomes_base}/databases"
+            arriba_ref          = "${params.genomes_base}/arriba"
+            ericscript_ref      = "${params.genomes_base}/ericscript/ericscript_db_homosapiens_ensembl73"
+            fusioncatcher_ref   = "${params.genomes_base}/fusioncatcher/README.txt"
+            star_fusion_ref     = "${params.genomes_base}/star-fusion/ctat_genome_lib_build_dir"
+        }
     }
-}
\ No newline at end of file
+}
diff --git a/docs/references.md b/docs/references.md
index d59d2dc5..23a4b8a9 100644
--- a/docs/references.md
+++ b/docs/references.md
@@ -27,6 +27,17 @@ nextflow run nf-core/rnafusion/download-references.nf \
 --outdir <PATH>
 ```
 
+## Download GRCh37 references
+
+```bash
+# GRCh38 genome assembly is used by default. To use the previous assembly specify it using the --genome flag
+nextflow run nf-core/rnafusion/download-references.nf \
+  --genome GRCh37 \
+  --download_all \
+  --outdir <PATH> \
+  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+```
+
 ## Tool reference requirements
 
 | Tool             |        FASTA       |         GTF        |     STAR-index     |        Other       |
diff --git a/docs/usage.md b/docs/usage.md
index 99ae7c4e..594c56d7 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -226,7 +226,7 @@ If enabled, executes `Ericscript` tool.
 
 ### `--fusioncatcher`
 
-If enabled, executes `Fusioncatcher` tool.
+If enabled, executes `Fusioncatcher` tool. N.B. that Fusioncatcher is not available when using the `GRCh37` genome assembly.
 
 - `--fusioncatcher_opt`
   - Specify additional parameters. For more info, please refer to the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md) of the tool.
@@ -308,7 +308,7 @@ Required reference in order to run `Fusioncatcher`.
 
 ### `--genome`
 
-This pipeline uses only `Homo Sapiens` version `GRCh38`. Also make sure to specify `--genomes_base`.
+This pipeline uses `Homo Sapiens` version `GRCh38` by default. Assembly `GRCh37` is optionally available. N.B. that using `GRCh37` precludes use of the `Fusioncatcher` tool. Also make sure to specify `--genomes_base`.
 
 ```bash
 --genome 'GRCh38' --genome_base '/path/to/references'
diff --git a/download-references.nf b/download-references.nf
index c9545338..9e2d62fe 100644
--- a/download-references.nf
+++ b/download-references.nf
@@ -28,16 +28,17 @@ def helpMessage() {
 
     Mandatory arguments:
       --outdir [path]               Output directory for downloading
-      
+
     Options:
       --download_all [bool]         Download all references
+      --genome [str]                Genome version: GRCh37 or GRCh38 (default)
       --reference_release [int]     Release number of Ensembl reference for FASTA and GTF
                                     Default: 97 -> ftp://ftp.ensembl.org/pub/release-97
       --base [bool]                 Download FASTA, GTF, cDNA
       --arriba [bool]               Download Arriba references
       --star_fusion [bool]          Build STAR-Fusion references from FASTA ANF GTF
       --fusioncatcher [bool]        Download Fusioncatcher references
-      --ericscript [bool]           Download Ericscript references 
+      --ericscript [bool]           Download Ericscript references
       --fusion_report [bool]        Download databases for fusion-report
       --cosmic_usr [str]            [Required with fusion_report] COSMIC username
       --cosmic_passwd [str]         [Required with fusion_report] COSMIC password
@@ -87,29 +88,34 @@ checkHostname()
 
 process download_base {
     publishDir "${params.outdir}/", mode: 'copy'
-    
+
     when:
     params.base || params.download_all
 
     output:
-    file "Homo_sapiens.GRCh38_r${params.reference_release}.all.fa" into fasta
-    file "Homo_sapiens.GRCh38_r${params.reference_release}.gtf" into gtf
-    file "Homo_sapiens.GRCh38_r${params.reference_release}.cdna.all.fa.gz" into transcript
+    file "Homo_sapiens.${params.genome}_r${params.reference_release}.all.fa" into fasta
+    file "Homo_sapiens.${params.genome}_r${params.reference_release}.gtf" into gtf
+    file "Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz" into transcript
 
     script:
     """
-    wget ftp://ftp.ensembl.org/pub/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.chromosome.{1..22}.fa.gz
-    wget ftp://ftp.ensembl.org/pub/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.chromosome.{MT,X,Y}.fa.gz
-    gunzip -c Homo_sapiens.GRCh38.dna.chromosome.* > Homo_sapiens.GRCh38_r${params.reference_release}.all.fa
-    wget ftp://ftp.ensembl.org/pub/release-${params.reference_release}/gtf/homo_sapiens/Homo_sapiens.GRCh38.${params.reference_release}.chr.gtf.gz -O Homo_sapiens.GRCh38_r${params.reference_release}.gtf.gz
-    gunzip Homo_sapiens.GRCh38_r${params.reference_release}.gtf.gz
-    wget ftp://ftp.ensembl.org/pub/release-${params.reference_release}/fasta/homo_sapiens/cdna/Homo_sapiens.GRCh38.cdna.all.fa.gz -O Homo_sapiens.GRCh38_r${params.reference_release}.cdna.all.fa.gz
+    if [[ ${params.genome} == "GRCh37" ]];then
+      BASEURL="ftp://ftp.ensembl.org/pub/grch37"
+    else
+      BASEURL="ftp://ftp.ensembl.org/pub"
+    fi
+    wget \$BASEURL/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
+    wget \$BASEURL/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
+    gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}_r${params.reference_release}.all.fa
+    wget \$BASEURL/release-${params.reference_release}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.*.chr.gtf.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
+    gunzip Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
+    wget \$BASEURL/release-${params.reference_release}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz
     """
 }
 
 process download_arriba {
     publishDir "${params.outdir}/arriba", mode: 'copy'
-    
+
     when:
     params.arriba || params.download_all
 
@@ -125,7 +131,7 @@ process download_arriba {
 
 process download_star_fusion {
     publishDir "${params.outdir}/star-fusion", mode: 'copy'
-    
+
     when:
     params.star_fusion || params.download_all
 
@@ -134,15 +140,21 @@ process download_star_fusion {
 
     script:
     """
-    aws s3 --no-sign-request --region ${params.awsregion} cp s3://ngi-igenomes/Homo_sapiens/Ensembl/GRCh38/Genome/CTAT/ctat_star_fusion_1_8_1.tar.gz .
-    tar -xf ctat_star_fusion_1_8_1.tar.gz --strip-components=5
-    rm ctat_star_fusion_1_8_1.tar.gz
+    if [[ ${params.genome} == "GRCh37" ]]; then
+      wget -O GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.9/GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz
+      tar -xf GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz --strip-components=5
+      rm GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz
+    else
+      aws s3 --no-sign-request --region ${params.awsregion} cp s3://ngi-igenomes/Homo_sapiens/Ensembl/GRCh38/Genome/CTAT/ctat_star_fusion_1_8_1.tar.gz .
+      tar -xf ctat_star_fusion_1_8_1.tar.gz --strip-components=5
+      rm ctat_star_fusion_1_8_1.tar.gz
+    fi
     """
 }
 
 process download_fusioncatcher {
     publishDir "${params.outdir}/fusioncatcher", mode: 'copy'
-    
+
     when:
     params.fusioncatcher || params.download_all
 
@@ -151,18 +163,22 @@ process download_fusioncatcher {
 
     script:
     """
-    wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.aa
-    wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ab
-    wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ac
-    wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ad
-    cat human_v98.tar.gz.* | tar xz
-    rm human_v98.tar*
+    if [[ ${params.genome} == "GRCh37" ]]; then
+      echo "FusionCatcher unavailable for GRCh37" > README.txt
+    else
+      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.aa
+      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ab
+      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ac
+      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ad
+      cat human_v98.tar.gz.* | tar xz
+      rm human_v98.tar*
+    fi
     """
 }
 
 process download_ericscript {
     publishDir "${params.outdir}/ericscript", mode: 'copy'
-    
+
     when:
     params.ericscript || params.download_all
 
@@ -173,14 +189,20 @@ process download_ericscript {
     """
     wget -N https://raw.githubusercontent.com/circulosmeos/gdown.pl/dfd6dc910a38a42d550397bb5c2335be2c4bcf54/gdown.pl
     chmod +x gdown.pl
-    ./gdown.pl "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIiUGt1SnFMZFg4TlE" ericscript_db_homosapiens_ensembl84.tar.bz2
-    tar jxf ericscript_db_homosapiens_ensembl84.tar.bz2
-    rm gdown.pl ericscript_db_homosapiens_ensembl84.tar.bz2
+    if [[ ${params.genome} == "GRCh37" ]]; then
+      ./gdown.pl "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIibDRIb0RFdHFlQmM" ericscript_db_homosapiens_ensembl73.tar.bz2
+      tar jxf ericscript_db_homosapiens_ensembl73.tar.bz2
+    else
+      ./gdown.pl "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIiUGt1SnFMZFg4TlE" ericscript_db_homosapiens_ensembl84.tar.bz2
+      tar jxf ericscript_db_homosapiens_ensembl84.tar.bz2
+    fi
+    rm gdown.pl ericscript_db_homosapiens_ensembl*.tar.bz2
     """
 }
 
 process download_databases {
     publishDir "${params.outdir}/databases", mode: 'copy'
+    executor 'local'
 
     when:
     params.fusion_report || params.download_all
diff --git a/main.nf b/main.nf
index b4f9151c..7d9f46aa 100644
--- a/main.nf
+++ b/main.nf
@@ -30,13 +30,13 @@ def helpMessage() {
     Mandatory arguments:
       --reads [file]                Path to input data (must be surrounded with quotes)
       -profile [str]                Configuration profile to use. Can use multiple (comma separated)
-                                    Available: docker, singularity, test, awsbatch, <institute> and more            
+                                    Available: docker, singularity, test, awsbatch, <institute> and more
       --reference_path [str]        Path to reference folder (includes fasta, gtf, fusion tool ref ...)
 
     Tool flags:
       --arriba [bool]                 Run Arriba
       --arriba_opt [str]              Specify extra parameters for Arriba
-      --ericscript [bool]             Run Ericscript  
+      --ericscript [bool]             Run Ericscript
       --fusioncatcher [bool]          Run FusionCatcher
       --fusioncatcher_opt [srt]       Specify extra parameters for FusionCatcher
       --fusion_report_opt [str]       Specify extra parameters for fusion-report
@@ -45,7 +45,7 @@ def helpMessage() {
       --squid [bool]                  Run Squid
       --star_fusion [bool]            Run STAR-Fusion
       --star_fusion_opt [str]         Specify extra parameters for STAR-Fusion
-    
+
     Visualization flags:
       --arriba_vis [bool]             Generate a PDF visualization per detected fusion
       --fusion_inspector [bool]       Run Fusion-Inspector
@@ -63,6 +63,7 @@ def helpMessage() {
       --transcript [file]             Path to transcript
 
     Options:
+      --genome [str]                  Genome version: GRCh37 or GRCh38 (default)
       --read_length [int]             Length of the reads. Default: 100
       --single_end [bool]             Specifies that the input is single-end reads
 
@@ -337,6 +338,7 @@ process arriba {
 
     script:
     def extra_params = params.arriba_opt ? params.arriba_opt : ''
+    blacklist = "${reference}/blacklist*${params.genome}*.tsv"
     """
     STAR \\
         --genomeDir ${star_index} \\
@@ -359,14 +361,14 @@ process arriba {
         --chimSegmentReadGapMax 3 \\
         --readFilesCommand zcat \\
         --sjdbOverhang ${params.read_length - 1} |
-    
+
     tee Aligned.out.bam |
 
     arriba \\
         -x /dev/stdin \\
         -a ${fasta} \\
         -g ${gtf} \\
-        -b ${reference}/blacklist_hg38_GRCh38_2018-11-04.tsv \\
+        -b ${blacklist} \\
         -o ${sample}_arriba.tsv -O ${sample}_discarded_arriba.tsv \\
         -T -P ${extra_params}
 
@@ -450,7 +452,7 @@ star_fusion_fusions = star_fusion_fusions.dump(tag:'star_fusion_fusions')
 process fusioncatcher {
     tag "${sample}"
     label 'process_high'
-    
+
     publishDir "${params.outdir}/tools/Fusioncatcher/${sample}", mode: 'copy'
 
     input:
@@ -461,7 +463,7 @@ process fusioncatcher {
         set val(sample), file("${sample}_fusioncatcher.txt") optional true into fusioncatcher_fusions
         file("*.{txt,zip,log}") into fusioncatcher_output
 
-    when: params.fusioncatcher || (params.fusioncatcher && params.debug)
+    when: params.genome != "GRCh37" && params.fusioncatcher || (params.fusioncatcher && params.debug)
 
     script:
     option = params.single_end ? reads[0] : "${reads[0]},${reads[1]}"
@@ -533,7 +535,7 @@ process pizzly {
         set val(sample), file(reads) from read_files_pizzly
         file(gtf) from ch_gtf
         file(transcript) from ch_transcript
-    
+
     output:
         set val(sample), file("${sample}_pizzly.txt") optional true into pizzly_fusions
         file("*.{json,txt}") into pizzly_output
@@ -575,7 +577,7 @@ process squid {
         set val(sample), file(reads) from read_files_squid
         file(star_index) from ch_star_index
         file(gtf) from ch_gtf
-    
+
     output:
         set val(sample), file("${sample}_fusions_annotated.txt") optional true into squid_fusions
         file("*.txt") into squid_output
@@ -633,7 +635,7 @@ process summary {
     tag "${sample}"
 
     publishDir "${params.outdir}/Reports/${sample}", mode: 'copy'
- 
+
     input:
         set val(sample), file(reads), file(arriba), file(ericscript), file(fusioncatcher), file(pizzly), file(squid), file(starfusion) from files_and_reports_summary
 
@@ -641,9 +643,9 @@ process summary {
         set val(sample), file("${sample}_fusion_list.tsv") into fusion_inspector_input_list
         file("${sample}_fusion_genes_mqc.json") into summary_fusions_mq
         file("*") into report
-    
+
     when: !params.debug && (running_tools.size() > 0)
-    
+
 
     script:
     def extra_params = params.fusion_report_opt ? params.fusion_report_opt : ''
@@ -658,6 +660,7 @@ process summary {
         ${tools} ${extra_params}
     mv fusion_list.tsv ${sample}_fusion_list.tsv
     mv fusion_genes_mqc.json ${sample}_fusion_genes_mqc.json
+    tar -czf ${sample}_fusion-report.tar.gz --exclude=${sample}* ./*
     """
 }
 
@@ -687,6 +690,8 @@ process arriba_visualization {
     script:
     def suff_mem = ("${(task.memory.toBytes() - 6000000000) / task.cpus}" > 2000000000) ? 'true' : 'false'
     def avail_mem = (task.memory && suff_mem) ? "-m" + "${(task.memory.toBytes() - 6000000000) / task.cpus}" : ''
+    cytobands = "${reference}/cytobands*${params.genome}*.tsv"
+    proteinDomains = "${reference}/protein_domains*${params.genome}*gff3"
     """
     samtools sort -@ ${task.cpus} ${avail_mem} -O bam ${bam} > Aligned.sortedByCoord.out.bam
     samtools index Aligned.sortedByCoord.out.bam
@@ -695,8 +700,8 @@ process arriba_visualization {
         --alignments=Aligned.sortedByCoord.out.bam \\
         --output=${sample}.pdf \\
         --annotation=${gtf} \\
-        --cytobands=${reference}/cytobands_hg38_GRCh38_2018-02-23.tsv \\
-        --proteinDomains=${reference}/protein_domains_hg38_GRCh38_2019-07-05.gff3
+        --cytobands=\$(echo ${cytobands}) \\
+        --proteinDomains=\$(echo ${proteinDomains})
     """
 }
 
@@ -734,7 +739,7 @@ process fusion_inspector {
         --CPU ${task.cpus} \\
         -O . \\
         --out_prefix finspector \\
-        --vis ${extra_params} 
+        --vis ${extra_params}
     """
 }
 

From ca6dc5d68192c15e54d950c5bd296d501c2ada56 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 30 Jul 2020 20:09:41 +0000
Subject: [PATCH 002/359] Template update for nf-core/tools version 1.10.1

---
 .github/.dockstore.yml                    |   5 +
 .github/CONTRIBUTING.md                   |   2 +-
 .github/ISSUE_TEMPLATE/bug_report.md      |  15 +-
 .github/ISSUE_TEMPLATE/feature_request.md |  12 +-
 .github/PULL_REQUEST_TEMPLATE.md          |  15 +-
 .github/workflows/awsfulltest.yml         |  40 ++++
 .github/workflows/awstest.yml             |  40 ++++
 .github/workflows/branch.yml              |  30 ++-
 .github/workflows/ci.yml                  |  47 +++-
 .github/workflows/linting.yml             |  13 +-
 .github/workflows/push_dockerhub.yml      |  39 ++++
 .gitignore                                |   1 +
 CHANGELOG.md                              |   8 +-
 CODE_OF_CONDUCT.md                        |   6 +-
 Dockerfile                                |  12 +-
 README.md                                 |  42 ++--
 bin/markdown_to_html.py                   |  43 ++--
 bin/scrape_software_versions.py           |  36 +--
 conf/igenomes.config                      |   1 +
 conf/test.config                          |   2 +-
 conf/test_full.config                     |  22 ++
 docs/README.md                            |  20 +-
 docs/output.md                            |  50 ++--
 docs/usage.md                             | 272 +++-------------------
 environment.yml                           |   2 +-
 main.nf                                   |  53 +++--
 nextflow.config                           |   9 +-
 nextflow_schema.json                      | 259 ++++++++++++++++++++
 28 files changed, 685 insertions(+), 411 deletions(-)
 create mode 100644 .github/.dockstore.yml
 create mode 100644 .github/workflows/awsfulltest.yml
 create mode 100644 .github/workflows/awstest.yml
 create mode 100644 .github/workflows/push_dockerhub.yml
 create mode 100644 conf/test_full.config
 create mode 100644 nextflow_schema.json

diff --git a/.github/.dockstore.yml b/.github/.dockstore.yml
new file mode 100644
index 00000000..030138a0
--- /dev/null
+++ b/.github/.dockstore.yml
@@ -0,0 +1,5 @@
+# Dockstore config version, not pipeline version
+version: 1.2
+workflows:
+  - subclass: nfl
+    primaryDescriptorPath: /nextflow.config
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 260f8fd6..e97e0285 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -54,4 +54,4 @@ These tests are run both with the latest available version of `Nextflow` and als
 
 ## Getting help
 
-For further information/help, please consult the [nf-core/rnafusion documentation](https://nf-co.re/nf-core/rnafusion/docs) and don't hesitate to get in touch on the nf-core Slack [#rnafusion](https://nfcore.slack.com/channels/rnafusion) channel ([join our Slack here](https://nf-co.re/join/slack)).
+For further information/help, please consult the [nf-core/rnafusion documentation](https://nf-co.re/rnafusion/docs) and don't hesitate to get in touch on the nf-core Slack [#rnafusion](https://nfcore.slack.com/channels/rnafusion) channel ([join our Slack here](https://nf-co.re/join/slack)).
diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md
index 45a4d208..c9950f88 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.md
+++ b/.github/ISSUE_TEMPLATE/bug_report.md
@@ -1,24 +1,27 @@
+<!--
 # nf-core/rnafusion bug report
 
 Hi there!
 
 Thanks for telling us about a problem with the pipeline.
+
 Please delete this text and anything that's not relevant from the template below:
+-->
 
-## Describe the bug
+## Description of the bug
 
-A clear and concise description of what the bug is.
+<!-- A clear and concise description of what the bug is. -->
 
 ## Steps to reproduce
 
 Steps to reproduce the behaviour:
 
-1. Command line: `nextflow run ...`
-2. See error: _Please provide your error message_
+1. Command line: <!-- [e.g. `nextflow run ...`] -->
+2. See error: <!-- [Please provide your error message] -->
 
 ## Expected behaviour
 
-A clear and concise description of what you expected to happen.
+<!-- A clear and concise description of what you expected to happen. -->
 
 ## System
 
@@ -39,4 +42,4 @@ A clear and concise description of what you expected to happen.
 
 ## Additional context
 
-Add any other context about the problem here.
+<!-- Add any other context about the problem here. -->
diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md
index 167d7f93..4560071e 100644
--- a/.github/ISSUE_TEMPLATE/feature_request.md
+++ b/.github/ISSUE_TEMPLATE/feature_request.md
@@ -1,24 +1,26 @@
+<!--
 # nf-core/rnafusion feature request
 
 Hi there!
 
 Thanks for suggesting a new feature for the pipeline!
 Please delete this text and anything that's not relevant from the template below:
+-->
 
 ## Is your feature request related to a problem? Please describe
 
-A clear and concise description of what the problem is.
+<!-- A clear and concise description of what the problem is. -->
 
-Ex. I'm always frustrated when [...]
+<!-- e.g. [I'm always frustrated when ...] -->
 
 ## Describe the solution you'd like
 
-A clear and concise description of what you want to happen.
+<!-- A clear and concise description of what you want to happen. -->
 
 ## Describe alternatives you've considered
 
-A clear and concise description of any alternative solutions or features you've considered.
+<!-- A clear and concise description of any alternative solutions or features you've considered. -->
 
 ## Additional context
 
-Add any other context about the feature request here.
+<!-- Add any other context about the feature request here. -->
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index e504e427..6e32aa01 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -1,3 +1,4 @@
+<!--
 # nf-core/rnafusion pull request
 
 Many thanks for contributing to nf-core/rnafusion!
@@ -5,15 +6,15 @@ Many thanks for contributing to nf-core/rnafusion!
 Please fill in the appropriate checklist below (delete whatever is not relevant).
 These are the most common things requested on pull requests (PRs).
 
+Remember that PRs should be made against the dev branch, unless you're preparing a pipeline release.
+
+Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+-->
+
 ## PR checklist
 
 - [ ] This comment contains a description of changes (with reason)
+- [ ] `CHANGELOG.md` is updated
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
-- [ ] If necessary, also make a PR on the [nf-core/rnafusion branch on the nf-core/test-datasets repo](https://github.com/nf-core/test-datasets/pull/new/nf-core/rnafusion)
-- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
-- [ ] Make sure your code lints (`nf-core lint .`).
 - [ ] Documentation in `docs` is updated
-- [ ] `CHANGELOG.md` is updated
-- [ ] `README.md` is updated
-
-**Learn more about contributing:** [CONTRIBUTING.md](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
\ No newline at end of file
+- [ ] If necessary, also make a PR on the [nf-core/rnafusion branch on the nf-core/test-datasets repo](https://github.com/nf-core/test-datasets/pull/new/nf-core/rnafusion)
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
new file mode 100644
index 00000000..f486dc8b
--- /dev/null
+++ b/.github/workflows/awsfulltest.yml
@@ -0,0 +1,40 @@
+name: nf-core AWS full size tests
+# This workflow is triggered on push to the master branch.
+# It runs the -profile 'test_full' on AWS batch
+
+on:
+  release:
+    types: [published]
+
+jobs:
+  run-awstest:
+    name: Run AWS full tests
+    if: github.repository == 'nf-core/rnafusion'
+    runs-on: ubuntu-latest
+    steps:
+      - name: Setup Miniconda
+        uses: goanpeca/setup-miniconda@v1.0.2
+        with:
+          auto-update-conda: true
+          python-version: 3.7
+      - name: Install awscli
+        run: conda install -c conda-forge awscli
+      - name: Start AWS batch job
+        # TODO nf-core: You can customise AWS full pipeline tests as required
+        # Add full size test data (but still relatively small datasets for few samples) 
+        # on the `test_full.config` test runs with only one set of parameters
+        # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command
+        env:
+          AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }}
+          AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }}
+          TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }}
+          AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }}
+          AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }}
+          AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }}
+        run: |
+          aws batch submit-job \
+            --region eu-west-1 \
+            --job-name nf-core-rnafusion \
+            --job-queue $AWS_JOB_QUEUE \
+            --job-definition $AWS_JOB_DEFINITION \
+            --container-overrides '{"command": ["nf-core/rnafusion", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/rnafusion/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/rnafusion/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
new file mode 100644
index 00000000..b687404c
--- /dev/null
+++ b/.github/workflows/awstest.yml
@@ -0,0 +1,40 @@
+name: nf-core AWS test
+# This workflow is triggered on push to the master branch.
+# It runs the -profile 'test' on AWS batch
+
+on:
+  push:
+    branches:
+      - master
+
+jobs:
+  run-awstest:
+    name: Run AWS tests
+    if: github.repository == 'nf-core/rnafusion'
+    runs-on: ubuntu-latest
+    steps:
+      - name: Setup Miniconda
+        uses: goanpeca/setup-miniconda@v1.0.2
+        with:
+          auto-update-conda: true
+          python-version: 3.7
+      - name: Install awscli
+        run: conda install -c conda-forge awscli
+      - name: Start AWS batch job
+        # TODO nf-core: You can customise CI pipeline run tests as required
+        # For example: adding multiple test runs with different parameters
+        # Remember that you can parallelise this by using strategy.matrix
+        env:
+          AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }}
+          AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }}
+          TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }}
+          AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }}
+          AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }}
+          AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }}
+        run: |
+          aws batch submit-job \
+          --region eu-west-1 \
+          --job-name nf-core-rnafusion \
+          --job-queue $AWS_JOB_QUEUE \
+          --job-definition $AWS_JOB_DEFINITION \
+          --container-overrides '{"command": ["nf-core/rnafusion", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/rnafusion/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/rnafusion/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'rnafusion/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index ed654eae..033f5230 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -3,14 +3,34 @@ name: nf-core branch protection
 # It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev`
 on:
   pull_request:
-    branches:
-    - master
+    branches: [master]
 
 jobs:
   test:
-    runs-on: ubuntu-18.04
+    runs-on: ubuntu-latest
     steps:
-      # PRs are only ok if coming from an nf-core `dev` branch or a fork `patch` branch
+      # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
       - name: Check PRs
+        if: github.repository == 'nf-core/rnafusion'
         run: |
-          { [[ $(git remote get-url origin) == *nf-core/rnafusion ]] && [[ ${GITHUB_HEAD_REF} = "dev" ]]; } || [[ ${GITHUB_HEAD_REF} == "patch" ]]
+          { [[ ${{github.event.pull_request.head.repo.full_name}} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
+
+
+      # If the above check failed, post a comment on the PR explaining the failure
+      - name: Post PR comment
+        if: failure()
+        uses: mshick/add-pr-comment@v1
+        with:
+          message: |
+            Hi @${{ github.event.pull_request.user.login }},
+
+            It looks like this pull-request is has been made against the ${{github.event.pull_request.head.repo.full_name}} `master` branch.
+            The `master` branch on nf-core repositories should always contain code from the latest release.
+            Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.head.repo.full_name}} `dev` branch.
+
+            You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page.
+
+            Thanks again for your contribution!
+          repo-token: ${{ secrets.GITHUB_TOKEN }}
+          allow-repeats: false
+
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index b08b3a2e..abcbc3b0 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -1,30 +1,55 @@
 name: nf-core CI
-# This workflow is triggered on pushes and PRs to the repository.
-# It runs the pipeline with the minimal test dataset to check that it completes without any syntax errors
-on: [push, pull_request]
+# This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors
+on:
+  push:
+    branches:
+      - dev
+  pull_request:
+  release:
+    types: [published]
 
 jobs:
   test:
+    name: Run workflow tests
+    # Only run on push if this is the nf-core dev branch (merged PRs)
+    if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}
+    runs-on: ubuntu-latest
     env:
       NXF_VER: ${{ matrix.nxf_ver }}
       NXF_ANSI_LOG: false
-    runs-on: ubuntu-latest
     strategy:
       matrix:
         # Nextflow versions: check pipeline minimum and current latest
         nxf_ver: ['19.10.0', '']
     steps:
-      - uses: actions/checkout@v2
-      - name: Install Nextflow
-        run: |
-          wget -qO- get.nextflow.io | bash
-          sudo mv nextflow /usr/local/bin/
+      - name: Check out pipeline code
+        uses: actions/checkout@v2
+
+      - name: Check if Dockerfile or Conda environment changed
+        uses: technote-space/get-diff-action@v1
+        with:
+          PREFIX_FILTER: |
+            Dockerfile
+            environment.yml
+
+      - name: Build new docker image
+        if: env.GIT_DIFF
+        run: docker build --no-cache . -t nfcore/rnafusion:dev
+
       - name: Pull docker image
+        if: ${{ !env.GIT_DIFF }}
         run: |
           docker pull nfcore/rnafusion:dev
           docker tag nfcore/rnafusion:dev nfcore/rnafusion:dev
+
+      - name: Install Nextflow
+        run: |
+          wget -qO- get.nextflow.io | bash
+          sudo mv nextflow /usr/local/bin/
+
       - name: Run pipeline with test data
+        # TODO nf-core: You can customise CI pipeline run tests as required
+        # For example: adding multiple test runs with different parameters
+        # Remember that you can parallelise this by using strategy.matrix
         run: |
-          # TODO nf-core: You can customise CI pipeline run tests as required
-          # (eg. adding multiple test runs with different parameters)
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 1e0827a8..eb66c144 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -33,18 +33,29 @@ jobs:
   nf-core:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v2
+
+      - name: Check out pipeline code
+        uses: actions/checkout@v2
+
       - name: Install Nextflow
         run: |
           wget -qO- get.nextflow.io | bash
           sudo mv nextflow /usr/local/bin/
+
       - uses: actions/setup-python@v1
         with:
           python-version: '3.6'
           architecture: 'x64'
+
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
           pip install nf-core
+
       - name: Run nf-core lint
+        env:
+          GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+          GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
         run: nf-core lint ${GITHUB_WORKSPACE}
+
diff --git a/.github/workflows/push_dockerhub.yml b/.github/workflows/push_dockerhub.yml
new file mode 100644
index 00000000..a11ca9ad
--- /dev/null
+++ b/.github/workflows/push_dockerhub.yml
@@ -0,0 +1,39 @@
+name: nf-core Docker push
+# This builds the docker image and pushes it to DockerHub
+# Runs on nf-core repo releases and push event to 'dev' branch (PR merges)
+on:
+  push:
+    branches:
+      - dev
+  release:
+    types: [published]
+
+push_dockerhub:
+  name: Push new Docker image to Docker Hub
+  runs-on: ubuntu-latest
+  # Only run for the nf-core repo, for releases and merged PRs
+  if: ${{ github.repository == 'nf-core/rnafusion' }}
+  env:
+    DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }}
+    DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }}
+  steps:
+    - name: Check out pipeline code
+      uses: actions/checkout@v2
+
+    - name: Build new docker image
+      run: docker build --no-cache . -t nfcore/rnafusion:latest
+
+    - name: Push Docker image to DockerHub (dev)
+      if: ${{ github.event_name == 'push' }}
+      run: |
+        echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
+        docker tag nfcore/rnafusion:latest nfcore/rnafusion:dev
+        docker push nfcore/rnafusion:dev
+
+    - name: Push Docker image to DockerHub (release)
+      if: ${{ github.event_name == 'release' }}
+      run: |
+        echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
+        docker push nfcore/rnafusion:latest
+        docker tag nfcore/rnafusion:latest nfcore/rnafusion:${{ github.event.release.tag_name }}
+        docker push nfcore/rnafusion:${{ github.event.release.tag_name }}
diff --git a/.gitignore b/.gitignore
index 6354f370..aa4bb5b3 100644
--- a/.gitignore
+++ b/.gitignore
@@ -5,4 +5,5 @@ results/
 .DS_Store
 tests/
 testing/
+testing*
 *.pyc
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 89cae718..06fdeeb8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -1,11 +1,11 @@
 # nf-core/rnafusion: Changelog
 
-The format is based on [Keep a Changelog](http://keepachangelog.com/en/1.0.0/)
-and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0.html).
+The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
+and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## vdev - [date]
+## v1.2.0 - [date]
 
-Initial release of nf-core/rnafusion, created with the [nf-core](http://nf-co.re/) template.
+Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
 
 ### `Added`
 
diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md
index cf930c8a..405fb1bf 100644
--- a/CODE_OF_CONDUCT.md
+++ b/CODE_OF_CONDUCT.md
@@ -40,7 +40,7 @@ Project maintainers who do not follow or enforce the Code of Conduct in good fai
 
 ## Attribution
 
-This Code of Conduct is adapted from the [Contributor Covenant][homepage], version 1.4, available at [http://contributor-covenant.org/version/1/4][version]
+This Code of Conduct is adapted from the [Contributor Covenant][homepage], version 1.4, available at [https://www.contributor-covenant.org/version/1/4/code-of-conduct/][version]
 
-[homepage]: http://contributor-covenant.org
-[version]: http://contributor-covenant.org/version/1/4/
+[homepage]: https://contributor-covenant.org
+[version]: https://www.contributor-covenant.org/version/1/4/code-of-conduct/
diff --git a/Dockerfile b/Dockerfile
index c3748956..95e5d5f9 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,13 +1,17 @@
-FROM nfcore/base:1.9
+FROM nfcore/base:1.10.1
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 
 # Install the conda environment
 COPY environment.yml /
-RUN conda env create -f /environment.yml && conda clean -a
+RUN conda env create --quiet -f /environment.yml && conda clean -a
 
 # Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-dev/bin:$PATH
+ENV PATH /opt/conda/envs/nf-core-rnafusion-1.2.0/bin:$PATH
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-dev > nf-core-rnafusion-dev.yml
+RUN conda env export --name nf-core-rnafusion-1.2.0 > nf-core-rnafusion-1.2.0.yml
+
+# Instruct R processes to use these empty files instead of clashing with a local version
+RUN touch .Rprofile
+RUN touch .Renviron
diff --git a/README.md b/README.md
index 183706e9..7f409297 100644
--- a/README.md
+++ b/README.md
@@ -6,8 +6,9 @@
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions)
 [![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A519.10.0-brightgreen.svg)](https://www.nextflow.io/)
 
-[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](http://bioconda.github.io/)
+[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/)
 [![Docker](https://img.shields.io/docker/automated/nfcore/rnafusion.svg)](https://hub.docker.com/r/nfcore/rnafusion)
+[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?logo=slack)](https://nfcore.slack.com/channels/rnafusion)
 
 ## Introduction
 
@@ -15,40 +16,31 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 
 ## Quick Start
 
-i. Install [`nextflow`](https://nf-co.re/usage/installation)
+1. Install [`nextflow`](https://nf-co.re/usage/installation)
 
-ii. Install either [`Docker`](https://docs.docker.com/engine/installation/) or [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) for full pipeline reproducibility (please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))
+2. Install either [`Docker`](https://docs.docker.com/engine/installation/) or [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
 
-iii. Download the pipeline and test it on a minimal dataset with a single command
+3. Download the pipeline and test it on a minimal dataset with a single command:
 
-```bash
-nextflow run nf-core/rnafusion -profile test,<docker/singularity/conda/institute>
-```
+    ```bash
+    nextflow run nf-core/rnafusion -profile test,<docker/singularity/conda/institute>
+    ```
 
-> Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
+    > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
 
-iv. Start running your own analysis!
+4. Start running your own analysis!
 
-<!-- TODO nf-core: Update the default command above used to run the pipeline -->
+    <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
 
-```bash
-nextflow run nf-core/rnafusion -profile <docker/singularity/conda/institute> --reads '*_R{1,2}.fastq.gz' --genome GRCh37
-```
+    ```bash
+    nextflow run nf-core/rnafusion -profile <docker/singularity/conda/institute> --input '*_R{1,2}.fastq.gz' --genome GRCh37
+    ```
 
 See [usage docs](docs/usage.md) for all of the available options when running the pipeline.
 
 ## Documentation
 
-The nf-core/rnafusion pipeline comes with documentation about the pipeline, found in the `docs/` directory:
-
-1. [Installation](https://nf-co.re/usage/installation)
-2. Pipeline configuration
-    * [Local installation](https://nf-co.re/usage/local_installation)
-    * [Adding your own system config](https://nf-co.re/usage/adding_own_config)
-    * [Reference genomes](https://nf-co.re/usage/reference_genomes)
-3. [Running the pipeline](docs/usage.md)
-4. [Output and how to interpret the results](docs/output.md)
-5. [Troubleshooting](https://nf-co.re/usage/troubleshooting)
+The nf-core/rnafusion pipeline comes with documentation about the pipeline which you can read at [https://nf-core/rnafusion/docs](https://nf-core/rnafusion/docs) or find in the [`docs/` directory](docs).
 
 <!-- TODO nf-core: Add a brief overview of what the pipeline does and how it works -->
 
@@ -60,7 +52,7 @@ nf-core/rnafusion was originally written by Martin Proks.
 
 If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).
 
-For further information or help, don't hesitate to get in touch on [Slack](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).
+For further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).
 
 ## Citation
 
@@ -73,5 +65,5 @@ You can cite the `nf-core` publication as follows:
 >
 > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.
 >
-> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).  
+> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).
 > ReadCube: [Full Access Link](https://rdcu.be/b1GjZ)
diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py
index 57cc4263..a26d1ff5 100755
--- a/bin/markdown_to_html.py
+++ b/bin/markdown_to_html.py
@@ -4,33 +4,23 @@
 import markdown
 import os
 import sys
+import io
+
 
 def convert_markdown(in_fn):
-    input_md = open(in_fn, mode="r", encoding="utf-8").read()
+    input_md = io.open(in_fn, mode="r", encoding="utf-8").read()
     html = markdown.markdown(
         "[TOC]\n" + input_md,
-        extensions = [
-            'pymdownx.extra',
-            'pymdownx.b64',
-            'pymdownx.highlight',
-            'pymdownx.emoji',
-            'pymdownx.tilde',
-            'toc'
-        ],
-        extension_configs = {
-            'pymdownx.b64': {
-                'base_path': os.path.dirname(in_fn)
-            },
-            'pymdownx.highlight': {
-                'noclasses': True
-            },
-            'toc': {
-                'title': 'Table of Contents'
-            }
-        }
+        extensions=["pymdownx.extra", "pymdownx.b64", "pymdownx.highlight", "pymdownx.emoji", "pymdownx.tilde", "toc"],
+        extension_configs={
+            "pymdownx.b64": {"base_path": os.path.dirname(in_fn)},
+            "pymdownx.highlight": {"noclasses": True},
+            "toc": {"title": "Table of Contents"},
+        },
     )
     return html
 
+
 def wrap_html(contents):
     header = """<!DOCTYPE html><html>
     <head>
@@ -83,18 +73,19 @@ def wrap_html(contents):
 
 def parse_args(args=None):
     parser = argparse.ArgumentParser()
-    parser.add_argument('mdfile', type=argparse.FileType('r'), nargs='?',
-                        help='File to convert. Defaults to stdin.')
-    parser.add_argument('-o', '--out', type=argparse.FileType('w'),
-                        default=sys.stdout,
-                        help='Output file name. Defaults to stdout.')
+    parser.add_argument("mdfile", type=argparse.FileType("r"), nargs="?", help="File to convert. Defaults to stdin.")
+    parser.add_argument(
+        "-o", "--out", type=argparse.FileType("w"), default=sys.stdout, help="Output file name. Defaults to stdout."
+    )
     return parser.parse_args(args)
 
+
 def main(args=None):
     args = parse_args(args)
     converted_md = convert_markdown(args.mdfile.name)
     html = wrap_html(converted_md)
     args.out.write(html)
 
-if __name__ == '__main__':
+
+if __name__ == "__main__":
     sys.exit(main())
diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
index 283f73fa..981e8de6 100755
--- a/bin/scrape_software_versions.py
+++ b/bin/scrape_software_versions.py
@@ -5,16 +5,16 @@
 
 # TODO nf-core: Add additional regexes for new tools in process get_software_versions
 regexes = {
-    'nf-core/rnafusion': ['v_pipeline.txt', r"(\S+)"],
-    'Nextflow': ['v_nextflow.txt', r"(\S+)"],
-    'FastQC': ['v_fastqc.txt', r"FastQC v(\S+)"],
-    'MultiQC': ['v_multiqc.txt', r"multiqc, version (\S+)"],
+    "nf-core/rnafusion": ["v_pipeline.txt", r"(\S+)"],
+    "Nextflow": ["v_nextflow.txt", r"(\S+)"],
+    "FastQC": ["v_fastqc.txt", r"FastQC v(\S+)"],
+    "MultiQC": ["v_multiqc.txt", r"multiqc, version (\S+)"],
 }
 results = OrderedDict()
-results['nf-core/rnafusion'] = '<span style="color:#999999;\">N/A</span>'
-results['Nextflow'] = '<span style="color:#999999;\">N/A</span>'
-results['FastQC'] = '<span style="color:#999999;\">N/A</span>'
-results['MultiQC'] = '<span style="color:#999999;\">N/A</span>'
+results["nf-core/rnafusion"] = '<span style="color:#999999;">N/A</span>'
+results["Nextflow"] = '<span style="color:#999999;">N/A</span>'
+results["FastQC"] = '<span style="color:#999999;">N/A</span>'
+results["MultiQC"] = '<span style="color:#999999;">N/A</span>'
 
 # Search each file using its regex
 for k, v in regexes.items():
@@ -30,10 +30,11 @@
 # Remove software set to false in results
 for k in list(results):
     if not results[k]:
-        del(results[k])
+        del results[k]
 
 # Dump to YAML
-print ('''
+print(
+    """
 id: 'software_versions'
 section_name: 'nf-core/rnafusion Software Versions'
 section_href: 'https://github.com/nf-core/rnafusion'
@@ -41,12 +42,13 @@
 description: 'are collected at run time from the software output.'
 data: |
     <dl class="dl-horizontal">
-''')
-for k,v in results.items():
-    print("        <dt>{}</dt><dd><samp>{}</samp></dd>".format(k,v))
-print ("    </dl>")
+"""
+)
+for k, v in results.items():
+    print("        <dt>{}</dt><dd><samp>{}</samp></dd>".format(k, v))
+print("    </dl>")
 
 # Write out regexes as csv file:
-with open('software_versions.csv', 'w') as f:
-    for k,v in results.items():
-        f.write("{}\t{}\n".format(k,v))
+with open("software_versions.csv", "w") as f:
+    for k, v in results.items():
+        f.write("{}\t{}\n".format(k, v))
diff --git a/conf/igenomes.config b/conf/igenomes.config
index 2de92422..caeafceb 100644
--- a/conf/igenomes.config
+++ b/conf/igenomes.config
@@ -340,6 +340,7 @@ params {
       gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
       bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
       mito_name   = "chrM"
+      macs_gsize  = "1.37e9"
     }
     'dm6' {
       fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
diff --git a/conf/test.config b/conf/test.config
index 3112650d..c84e3197 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -19,7 +19,7 @@ params {
   // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
   // TODO nf-core: Give any required params for the test so that command line flags are not needed
   single_end = false
-  readPaths = [
+  input_paths = [
     ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']],
     ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']]
   ]
diff --git a/conf/test_full.config b/conf/test_full.config
new file mode 100644
index 00000000..96b058e0
--- /dev/null
+++ b/conf/test_full.config
@@ -0,0 +1,22 @@
+/*
+ * -------------------------------------------------
+ *  Nextflow config file for running full-size tests
+ * -------------------------------------------------
+ * Defines bundled input files and everything required
+ * to run a full size pipeline test. Use as follows:
+ *   nextflow run nf-core/rnafusion -profile test_full,<docker/singularity>
+ */
+
+params {
+  config_profile_name = 'Full test profile'
+  config_profile_description = 'Full test dataset to check pipeline function'
+
+  // Input data for full size test
+  // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA)
+  // TODO nf-core: Give any required params for the test so that command line flags are not needed
+  single_end = false
+  readPaths = [
+    ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']],
+    ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']]
+  ]
+}
diff --git a/docs/README.md b/docs/README.md
index 62f94b57..b8d76fa6 100644
--- a/docs/README.md
+++ b/docs/README.md
@@ -1,12 +1,12 @@
 # nf-core/rnafusion: Documentation
 
-The nf-core/rnafusion documentation is split into the following files:
-
-1. [Installation](https://nf-co.re/usage/installation)
-2. Pipeline configuration
-    * [Local installation](https://nf-co.re/usage/local_installation)
-    * [Adding your own system config](https://nf-co.re/usage/adding_own_config)
-    * [Reference genomes](https://nf-co.re/usage/reference_genomes)
-3. [Running the pipeline](usage.md)
-4. [Output and how to interpret the results](output.md)
-5. [Troubleshooting](https://nf-co.re/usage/troubleshooting)
+The nf-core/rnafusion documentation is split into the following pages:
+
+<!-- TODO nf-core: If you write more documentation pages, add them to the docs index page here -->
+
+* [Usage](usage.md)
+  * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
+* [Output](output.md)
+  * An overview of the different results produced by the pipeline and how to interpret them.
+
+You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re)
diff --git a/docs/output.md b/docs/output.md
index 86dae502..0dbd4f72 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -2,6 +2,8 @@
 
 This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline.
 
+The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory.
+
 <!-- TODO nf-core: Write this documentation describing your workflow's output -->
 
 ## Pipeline overview
@@ -9,35 +11,47 @@ This document describes the output produced by the pipeline. Most of the plots a
 The pipeline is built using [Nextflow](https://www.nextflow.io/)
 and processes data using the following steps:
 
-* [FastQC](#fastqc) - read quality control
-* [MultiQC](#multiqc) - aggregate report, describing results of the whole pipeline
+* [FastQC](#fastqc) - Read quality control
+* [MultiQC](#multiqc) - Aggregate report describing results from the whole pipeline
+* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ## FastQC
 
-[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your reads. It provides information about the quality score distribution across your reads, the per base sequence content (%T/A/G/C). You get information about adapter contamination and other overrepresented sequences.
+[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences.
 
-For further reading and documentation see the [FastQC help](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
+For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
 
-> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. To see how your reads look after trimming, look at the FastQC reports in the `trim_galore` directory.
+**Output files:**
 
-**Output directory: `results/fastqc`**
+* `fastqc/`
+  * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files.
+* `fastqc/zips/`
+  * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
-* `sample_fastqc.html`
-  * FastQC report, containing quality metrics for your untrimmed raw fastq files
-* `zips/sample_fastqc.zip`
-  * zip file containing the FastQC report, tab-delimited data file and plot images
+> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
 
 ## MultiQC
 
-[MultiQC](http://multiqc.info) is a visualisation tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in within the report data directory.
+[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarizing all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.
+
+The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability.
+
+For more information about how to use MultiQC reports, see [https://multiqc.info](https://multiqc.info).
+
+**Output files:**
+
+* `multiqc/`  
+  * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
+  * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
+  * `multiqc_plots/`: directory containing static images from the report in various formats.
 
-The pipeline has special steps which allow the software versions used to be reported in the MultiQC output for future traceability.
+## Pipeline information
 
-**Output directory: `results/multiqc`**
+[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
 
-* `Project_multiqc_report.html`
-  * MultiQC report - a standalone HTML file that can be viewed in your web browser
-* `Project_multiqc_data/`
-  * Directory containing parsed statistics from the different tools used in the pipeline
+**Output files:**
 
-For more information about how to use MultiQC reports, see [http://multiqc.info](http://multiqc.info)
+* `pipeline_info/`
+  * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
+  * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`.
+  * Documentation for interpretation of results in HTML format: `results_description.html`.
diff --git a/docs/usage.md b/docs/usage.md
index 94bf53a8..40c74e70 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -1,62 +1,15 @@
 # nf-core/rnafusion: Usage
 
-## Table of contents
-
-* [Table of contents](#table-of-contents)
-* [Introduction](#introduction)
-* [Running the pipeline](#running-the-pipeline)
-  * [Updating the pipeline](#updating-the-pipeline)
-  * [Reproducibility](#reproducibility)
-* [Main arguments](#main-arguments)
-  * [`-profile`](#-profile)
-  * [`--reads`](#--reads)
-  * [`--single_end`](#--single_end)
-* [Reference genomes](#reference-genomes)
-  * [`--genome` (using iGenomes)](#--genome-using-igenomes)
-  * [`--fasta`](#--fasta)
-  * [`--igenomes_ignore`](#--igenomes_ignore)
-* [Job resources](#job-resources)
-  * [Automatic resubmission](#automatic-resubmission)
-  * [Custom resource requests](#custom-resource-requests)
-* [AWS Batch specific parameters](#aws-batch-specific-parameters)
-  * [`--awsqueue`](#--awsqueue)
-  * [`--awsregion`](#--awsregion)
-  * [`--awscli`](#--awscli)
-* [Other command line parameters](#other-command-line-parameters)
-  * [`--outdir`](#--outdir)
-  * [`--email`](#--email)
-  * [`--email_on_fail`](#--email_on_fail)
-  * [`--max_multiqc_email_size`](#--max_multiqc_email_size)
-  * [`-name`](#-name)
-  * [`-resume`](#-resume)
-  * [`-c`](#-c)
-  * [`--custom_config_version`](#--custom_config_version)
-  * [`--custom_config_base`](#--custom_config_base)
-  * [`--max_memory`](#--max_memory)
-  * [`--max_time`](#--max_time)
-  * [`--max_cpus`](#--max_cpus)
-  * [`--plaintext_email`](#--plaintext_email)
-  * [`--monochrome_logs`](#--monochrome_logs)
-  * [`--multiqc_config`](#--multiqc_config)
-
 ## Introduction
 
-Nextflow handles job submissions on SLURM or other environments, and supervises running the jobs. Thus the Nextflow process must run until the pipeline is finished. We recommend that you put the process running in the background through `screen` / `tmux` or similar tool. Alternatively you can run nextflow within a cluster job submitted your job scheduler.
-
-It is recommended to limit the Nextflow Java virtual machines memory. We recommend adding the following line to your environment (typically in `~/.bashrc` or `~./bash_profile`):
-
-```bash
-NXF_OPTS='-Xms1g -Xmx4g'
-```
-
-<!-- TODO nf-core: Document required command line parameters to run the pipeline-->
+<!-- TODO nf-core: Add documentation about anything specific to running your pipeline. For general topics, please point to (and add to) the main nf-core website. -->
 
 ## Running the pipeline
 
 The typical command for running the pipeline is as follows:
 
 ```bash
-nextflow run nf-core/rnafusion --reads '*_R{1,2}.fastq.gz' -profile docker
+nextflow run nf-core/rnafusion --input '*_R{1,2}.fastq.gz' -profile docker
 ```
 
 This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
@@ -86,7 +39,9 @@ First, go to the [nf-core/rnafusion releases page](https://github.com/nf-core/rn
 
 This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future.
 
-## Main arguments
+## Core Nextflow arguments
+
+> **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen).
 
 ### `-profile`
 
@@ -104,11 +59,11 @@ They are loaded in sequence, so later profiles can overwrite earlier profiles.
 If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended.
 
 * `docker`
-  * A generic configuration profile to be used with [Docker](http://docker.com/)
-  * Pulls software from dockerhub: [`nfcore/rnafusion`](http://hub.docker.com/r/nfcore/rnafusion/)
+  * A generic configuration profile to be used with [Docker](https://docker.com/)
+  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
 * `singularity`
-  * A generic configuration profile to be used with [Singularity](http://singularity.lbl.gov/)
-  * Pulls software from DockerHub: [`nfcore/rnafusion`](http://hub.docker.com/r/nfcore/rnafusion/)
+  * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
+  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
 * `conda`
   * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker or Singularity.
   * A generic configuration profile to be used with [Conda](https://conda.io/docs/)
@@ -117,215 +72,50 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
   * A profile with a complete configuration for automated testing
   * Includes links to test data so needs no other parameters
 
-<!-- TODO nf-core: Document required command line parameters -->
-
-### `--reads`
-
-Use this to specify the location of your input FastQ files. For example:
-
-```bash
---reads 'path/to/data/sample_*_{1,2}.fastq'
-```
-
-Please note the following requirements:
-
-1. The path must be enclosed in quotes
-2. The path must have at least one `*` wildcard character
-3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.
-
-If left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`
-
-### `--single_end`
-
-By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--reads`. For example:
-
-```bash
---single_end --reads '*.fastq'
-```
-
-It is not possible to run a mixture of single-end and paired-end files in one run.
-
-## Reference genomes
-
-The pipeline config files come bundled with paths to the illumina iGenomes reference index files. If running with docker or AWS, the configuration is set up to use the [AWS-iGenomes](https://ewels.github.io/AWS-iGenomes/) resource.
-
-### `--genome` (using iGenomes)
+### `-resume`
 
-There are 31 different species supported in the iGenomes references. To run the pipeline, you must specify which to use with the `--genome` flag.
+Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously.
 
-You can find the keys to specify the genomes in the [iGenomes config file](../conf/igenomes.config). Common genomes that are supported are:
+You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names.
 
-* Human
-  * `--genome GRCh37`
-* Mouse
-  * `--genome GRCm38`
-* _Drosophila_
-  * `--genome BDGP6`
-* _S. cerevisiae_
-  * `--genome 'R64-1-1'`
+### `-c`
 
-> There are numerous others - check the config file for more.
+Specify the path to a specific config file (this is a core NextFlow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information.
 
-Note that you can use the same configuration setup to save sets of reference files for your own use, even if they are not part of the iGenomes resource. See the [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for instructions on where to save such a file.
+#### Custom resource requests
 
-The syntax for this reference configuration is as follows:
+Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped.
 
-<!-- TODO nf-core: Update reference genome example according to what is needed -->
+Whilst these default requirements will hopefully work for most people with most data, you may find that you want to customise the compute resources that the pipeline requests. You can do this by creating a custom config file. For example, to give the workflow process `star` 32GB of memory, you could use the following config:
 
 ```nextflow
-params {
-  genomes {
-    'GRCh37' {
-      fasta   = '<path to the genome fasta file>' // Used if no star index given
-    }
-    // Any number of additional genomes, key is used with --genome
+process {
+  withName: star {
+    memory = 32.GB
   }
 }
 ```
 
-<!-- TODO nf-core: Describe reference path flags -->
-
-### `--fasta`
-
-If you prefer, you can specify the full path to your reference genome when you run the pipeline:
-
-```bash
---fasta '[path to Fasta reference]'
-```
-
-### `--igenomes_ignore`
-
-Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`.
-
-## Job resources
-
-### Automatic resubmission
-
-Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped.
-
-### Custom resource requests
-
-Wherever process-specific requirements are set in the pipeline, the default value can be changed by creating a custom config file. See the files hosted at [`nf-core/configs`](https://github.com/nf-core/configs/tree/master/conf) for examples.
+See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information.
 
 If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition below). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile.
 
-If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack).
-
-## AWS Batch specific parameters
-
-Running the pipeline on AWS Batch requires a couple of specific parameters to be set according to your AWS Batch configuration. Please use [`-profile awsbatch`](https://github.com/nf-core/configs/blob/master/conf/awsbatch.config) and then specify all of the following parameters.
-
-### `--awsqueue`
-
-The JobQueue that you intend to use on AWS Batch.
+If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs).
 
-### `--awsregion`
+### Running in the background
 
-The AWS region in which to run your job. Default is set to `eu-west-1` but can be adjusted to your needs.
+Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished.
 
-### `--awscli`
+The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file.
 
-The [AWS CLI](https://www.nextflow.io/docs/latest/awscloud.html#aws-cli-installation) path in your custom AMI. Default: `/home/ec2-user/miniconda/bin/aws`.
+Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time.
+Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs).
 
-Please make sure to also set the `-w/--work-dir` and `--outdir` parameters to a S3 storage bucket of your choice - you'll get an error message notifying you if you didn't.
+#### Nextflow memory requirements
 
-## Other command line parameters
-
-<!-- TODO nf-core: Describe any other command line flags here -->
-
-### `--outdir`
-
-The output directory where the results will be saved.
-
-### `--email`
-
-Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.
-
-### `--email_on_fail`
-
-This works exactly as with `--email`, except emails are only sent if the workflow is not successful.
-
-### `--max_multiqc_email_size`
-
-Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB).
-
-### `-name`
-
-Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic.
-
-This is used in the MultiQC report (if not default) and in the summary HTML / e-mail (always).
-
-**NB:** Single hyphen (core Nextflow option)
-
-### `-resume`
-
-Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously.
-
-You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names.
-
-**NB:** Single hyphen (core Nextflow option)
-
-### `-c`
-
-Specify the path to a specific config file (this is a core NextFlow command).
-
-**NB:** Single hyphen (core Nextflow option)
-
-Note - you can use this to override pipeline defaults.
-
-### `--custom_config_version`
-
-Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.
-
-```bash
-## Download and use config file with following git commid id
---custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96
-```
-
-### `--custom_config_base`
-
-If you're running offline, nextflow will not be able to fetch the institutional config files
-from the internet. If you don't need them, then this is not a problem. If you do need them,
-you should download the files from the repo and tell nextflow where to find them with the
-`custom_config_base` option. For example:
+In some cases, the Nextflow Java virtual machines can start to request a large amount of memory.
+We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`):
 
 ```bash
-## Download and unzip the config files
-cd /path/to/my/configs
-wget https://github.com/nf-core/configs/archive/master.zip
-unzip master.zip
-
-## Run the pipeline
-cd /path/to/my/data
-nextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/
+NXF_OPTS='-Xms1g -Xmx4g'
 ```
-
-> Note that the nf-core/tools helper package has a `download` command to download all required pipeline
-> files + singularity containers + institutional configs in one go for you, to make this process easier.
-
-### `--max_memory`
-
-Use to set a top-limit for the default memory requirement for each process.
-Should be a string in the format integer-unit. eg. `--max_memory '8.GB'`
-
-### `--max_time`
-
-Use to set a top-limit for the default time requirement for each process.
-Should be a string in the format integer-unit. eg. `--max_time '2.h'`
-
-### `--max_cpus`
-
-Use to set a top-limit for the default CPU requirement for each process.
-Should be a string in the format integer-unit. eg. `--max_cpus 1`
-
-### `--plaintext_email`
-
-Set to receive plain-text e-mails instead of HTML formatted.
-
-### `--monochrome_logs`
-
-Set to disable colourful command line output and live life in monochrome.
-
-### `--multiqc_config`
-
-Specify a path to a custom MultiQC configuration file.
diff --git a/environment.yml b/environment.yml
index 2be75611..5191a7a3 100644
--- a/environment.yml
+++ b/environment.yml
@@ -1,6 +1,6 @@
 # You can use this file to create a conda environment for this pipeline:
 #   conda env create -f environment.yml
-name: nf-core-rnafusion-dev
+name: nf-core-rnafusion-1.2.0
 channels:
   - conda-forge
   - bioconda
diff --git a/main.nf b/main.nf
index d7801386..bd2a1b35 100644
--- a/main.nf
+++ b/main.nf
@@ -18,12 +18,12 @@ def helpMessage() {
 
     The typical command for running the pipeline is as follows:
 
-    nextflow run nf-core/rnafusion --reads '*_R{1,2}.fastq.gz' -profile docker
+    nextflow run nf-core/rnafusion --input '*_R{1,2}.fastq.gz' -profile docker
 
     Mandatory arguments:
-      --reads [file]                Path to input data (must be surrounded with quotes)
-      -profile [str]                Configuration profile to use. Can use multiple (comma separated)
-                                    Available: conda, docker, singularity, test, awsbatch, <institute> and more
+      --input [file]                  Path to input data (must be surrounded with quotes)
+      -profile [str]                  Configuration profile to use. Can use multiple (comma separated)
+                                      Available: conda, docker, singularity, test, awsbatch, <institute> and more
 
     Options:
       --genome [str]                  Name of iGenomes reference
@@ -34,9 +34,10 @@ def helpMessage() {
 
     Other options:
       --outdir [file]                 The output directory where the results will be saved
+      --publish_dir_mode [str]        Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy)
       --email [email]                 Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits
       --email_on_fail [email]         Same as --email, except only send mail if the workflow is not successful
-      --max_multiqc_email_size [str]  Theshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB)
+      --max_multiqc_email_size [str]  Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB)
       -name [str]                     Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic
 
     AWSBatch options:
@@ -73,12 +74,13 @@ params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false :
 if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) }
 
 // Has the run name been specified by the user?
-//  this has the bonus effect of catching both -name and --name
+// this has the bonus effect of catching both -name and --name
 custom_runName = params.name
 if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) {
     custom_runName = workflow.runName
 }
 
+// Check AWS batch settings
 if (workflow.profile.contains('awsbatch')) {
     // AWSBatch sanity checking
     if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!"
@@ -93,28 +95,29 @@ if (workflow.profile.contains('awsbatch')) {
 ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true)
 ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty()
 ch_output_docs = file("$baseDir/docs/output.md", checkIfExists: true)
+ch_output_docs_images = file("$baseDir/docs/images/", checkIfExists: true)
 
 /*
  * Create a channel for input read files
  */
-if (params.readPaths) {
+if (params.input_paths) {
     if (params.single_end) {
         Channel
-            .from(params.readPaths)
+            .from(params.input_paths)
             .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] }
-            .ifEmpty { exit 1, "params.readPaths was empty - no input files supplied" }
+            .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" }
             .into { ch_read_files_fastqc; ch_read_files_trimming }
     } else {
         Channel
-            .from(params.readPaths)
+            .from(params.input_paths)
             .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] }
-            .ifEmpty { exit 1, "params.readPaths was empty - no input files supplied" }
+            .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" }
             .into { ch_read_files_fastqc; ch_read_files_trimming }
     }
 } else {
     Channel
-        .fromFilePairs(params.reads, size: params.single_end ? 1 : 2)
-        .ifEmpty { exit 1, "Cannot find any reads matching: ${params.reads}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." }
+        .fromFilePairs(params.input, size: params.single_end ? 1 : 2)
+        .ifEmpty { exit 1, "Cannot find any reads matching: ${params.input}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." }
         .into { ch_read_files_fastqc; ch_read_files_trimming }
 }
 
@@ -124,7 +127,7 @@ def summary = [:]
 if (workflow.revision) summary['Pipeline Release'] = workflow.revision
 summary['Run Name']         = custom_runName ?: workflow.runName
 // TODO nf-core: Report custom parameters here
-summary['Reads']            = params.reads
+summary['Reads']            = params.input
 summary['Fasta Ref']        = params.fasta
 summary['Data Type']        = params.single_end ? 'Single-End' : 'Paired-End'
 summary['Max Resources']    = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job"
@@ -140,9 +143,10 @@ if (workflow.profile.contains('awsbatch')) {
     summary['AWS CLI']      = params.awscli
 }
 summary['Config Profile'] = workflow.profile
-if (params.config_profile_description) summary['Config Description'] = params.config_profile_description
-if (params.config_profile_contact)     summary['Config Contact']     = params.config_profile_contact
-if (params.config_profile_url)         summary['Config URL']         = params.config_profile_url
+if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description
+if (params.config_profile_contact)     summary['Config Profile Contact']     = params.config_profile_contact
+if (params.config_profile_url)         summary['Config Profile URL']         = params.config_profile_url
+summary['Config Files'] = workflow.configFiles.join(', ')
 if (params.email || params.email_on_fail) {
     summary['E-mail Address']    = params.email
     summary['E-mail on failure'] = params.email_on_fail
@@ -174,7 +178,7 @@ Channel.from(summary.collect{ [it.key, it.value] })
  * Parse software version numbers
  */
 process get_software_versions {
-    publishDir "${params.outdir}/pipeline_info", mode: 'copy',
+    publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode,
         saveAs: { filename ->
                       if (filename.indexOf(".csv") > 0) filename
                       else null
@@ -201,7 +205,7 @@ process get_software_versions {
 process fastqc {
     tag "$name"
     label 'process_medium'
-    publishDir "${params.outdir}/fastqc", mode: 'copy',
+    publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode,
         saveAs: { filename ->
                       filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename"
                 }
@@ -222,7 +226,7 @@ process fastqc {
  * STEP 2 - MultiQC
  */
 process multiqc {
-    publishDir "${params.outdir}/MultiQC", mode: 'copy'
+    publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode
 
     input:
     file (multiqc_config) from ch_multiqc_config
@@ -251,10 +255,11 @@ process multiqc {
  * STEP 3 - Output Description HTML
  */
 process output_documentation {
-    publishDir "${params.outdir}/pipeline_info", mode: 'copy'
+    publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode
 
     input:
     file output_docs from ch_output_docs
+    file images from ch_output_docs_images
 
     output:
     file "results_description.html"
@@ -345,7 +350,11 @@ workflow.onComplete {
             log.info "[nf-core/rnafusion] Sent summary e-mail to $email_address (sendmail)"
         } catch (all) {
             // Catch failures and try with plaintext
-            [ 'mail', '-s', subject, email_address ].execute() << email_txt
+            def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
+            if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) {
+              mail_cmd += [ '-A', mqc_report ]
+            }
+            mail_cmd.execute() << email_html
             log.info "[nf-core/rnafusion] Sent summary e-mail to $email_address (mail)"
         }
     }
diff --git a/nextflow.config b/nextflow.config
index 49451c6c..2906b885 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -11,9 +11,10 @@ params {
   // Workflow flags
   // TODO nf-core: Specify your pipeline's command line flags
   genome = false
-  reads = "data/*{1,2}.fastq.gz"
+  input = "data/*{1,2}.fastq.gz"
   single_end = false
   outdir = './results'
+  publish_dir_mode = 'copy'
 
   // Boilerplate options
   name = false
@@ -78,9 +79,11 @@ if (!params.igenomes_ignore) {
   includeConfig 'conf/igenomes.config'
 }
 
-// Export this variable to prevent local Python libraries from conflicting with those in the container
+// Export these variables to prevent local Python/R libraries from conflicting with those in the container
 env {
   PYTHONNOUSERSITE = 1
+  R_PROFILE_USER = "/.Rprofile"
+  R_ENVIRON_USER = "/.Renviron"
 }
 
 // Capture exit codes from upstream processes when piping
@@ -110,7 +113,7 @@ manifest {
   description = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
   mainScript = 'main.nf'
   nextflowVersion = '>=19.10.0'
-  version = 'dev'
+  version = '1.2.0'
 }
 
 // Function to ensure that resource requirements don't go beyond
diff --git a/nextflow_schema.json b/nextflow_schema.json
new file mode 100644
index 00000000..abf782b6
--- /dev/null
+++ b/nextflow_schema.json
@@ -0,0 +1,259 @@
+{
+    "$schema": "https://json-schema.org/draft-07/schema",
+    "$id": "https://raw.githubusercontent.com/nf-core/rnafusion/master/nextflow_schema.json",
+    "title": "nf-core/rnafusion pipeline parameters",
+    "description": "Nextflow rnafusion analysis pipeline, part of the nf-core community.",
+    "type": "object",
+    "definitions": {
+        "input_output_options": {
+            "title": "Input/output options",
+            "type": "object",
+            "fa_icon": "fas fa-terminal",
+            "description": "Define where the pipeline should find input data and save output data.",
+            "required": [
+                "input"
+            ],
+            "properties": {
+                "input": {
+                    "type": "string",
+                    "fa_icon": "fas fa-dna",
+                    "description": "Input FastQ files.",
+                    "help_text": "Use this to specify the location of your input FastQ files. For example:\n\n```bash\n--input 'path/to/data/sample_*_{1,2}.fastq'\n```\n\nPlease note the following requirements:\n\n1. The path must be enclosed in quotes\n2. The path must have at least one `*` wildcard character\n3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.\n\nIf left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`"
+                },
+                "single_end": {
+                    "type": "boolean",
+                    "description": "Specifies that the input is single-end reads.",
+                    "fa_icon": "fas fa-align-center",
+                    "help_text": "By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:\n\n```bash\n--single_end --input '*.fastq'\n```\n\nIt is not possible to run a mixture of single-end and paired-end files in one run."
+                },
+                "outdir": {
+                    "type": "string",
+                    "description": "The output directory where the results will be saved.",
+                    "default": "./results",
+                    "fa_icon": "fas fa-folder-open"
+                },
+                "email": {
+                    "type": "string",
+                    "description": "Email address for completion summary.",
+                    "fa_icon": "fas fa-envelope",
+                    "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.",
+                    "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$"
+                }
+            }
+        },
+        "reference_genome_options": {
+            "title": "Reference genome options",
+            "type": "object",
+            "fa_icon": "fas fa-dna",
+            "description": "Options for the reference genome indices used to align reads.",
+            "properties": {
+                "genome": {
+                    "type": "string",
+                    "description": "Name of iGenomes reference.",
+                    "fa_icon": "fas fa-book",
+                    "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`.\n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details."
+                },
+                "fasta": {
+                    "type": "string",
+                    "fa_icon": "fas fa-font",
+                    "description": "Path to FASTA genome file.",
+                    "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible."
+                },
+                "igenomes_base": {
+                    "type": "string",
+                    "description": "Directory / URL base for iGenomes references.",
+                    "default": "s3://ngi-igenomes/igenomes/",
+                    "fa_icon": "fas fa-cloud-download-alt",
+                    "hidden": true
+                },
+                "igenomes_ignore": {
+                    "type": "boolean",
+                    "description": "Do not load the iGenomes reference config.",
+                    "fa_icon": "fas fa-ban",
+                    "hidden": true,
+                    "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
+                }
+            }
+        },
+        "generic_options": {
+            "title": "Generic options",
+            "type": "object",
+            "fa_icon": "fas fa-file-import",
+            "description": "Less common options for the pipeline, typically set in a config file.",
+            "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
+            "properties": {
+                "help": {
+                    "type": "boolean",
+                    "description": "Display help text.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-question-circle"
+                },
+                "publish_dir_mode": {
+                    "type": "string",
+                    "default": "copy",
+                    "hidden": true,
+                    "description": "Method used to save pipeline results to output directory.",
+                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
+                    "fa_icon": "fas fa-copy",
+                    "enum": [
+                        "symlink",
+                        "rellink",
+                        "link",
+                        "copy",
+                        "copyNoFollow",
+                        "mov"
+                    ]
+                },
+                "name": {
+                    "type": "string",
+                    "description": "Workflow name.",
+                    "fa_icon": "fas fa-fingerprint",
+                    "hidden": true,
+                    "help_text": "A custom name for the pipeline run. Unlike the core nextflow `-name` option with one hyphen this parameter can be reused multiple times, for example if using `-resume`. Passed through to steps such as MultiQC and used for things like report filenames and titles."
+                },
+                "email_on_fail": {
+                    "type": "string",
+                    "description": "Email address for completion summary, only when pipeline fails.",
+                    "fa_icon": "fas fa-exclamation-triangle",
+                    "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$",
+                    "hidden": true,
+                    "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful."
+                },
+                "plaintext_email": {
+                    "type": "boolean",
+                    "description": "Send plain-text email instead of HTML.",
+                    "fa_icon": "fas fa-remove-format",
+                    "hidden": true,
+                    "help_text": "Set to receive plain-text e-mails instead of HTML formatted."
+                },
+                "max_multiqc_email_size": {
+                    "type": "string",
+                    "description": "File size limit when attaching MultiQC reports to summary emails.",
+                    "default": "25.MB",
+                    "fa_icon": "fas fa-file-upload",
+                    "hidden": true,
+                    "help_text": "If file generated by pipeline exceeds the threshold, it will not be attached."
+                },
+                "monochrome_logs": {
+                    "type": "boolean",
+                    "description": "Do not use coloured log outputs.",
+                    "fa_icon": "fas fa-palette",
+                    "hidden": true,
+                    "help_text": "Set to disable colourful command line output and live life in monochrome."
+                },
+                "multiqc_config": {
+                    "type": "string",
+                    "description": "Custom config file to supply to MultiQC.",
+                    "fa_icon": "fas fa-cog",
+                    "hidden": true
+                },
+                "tracedir": {
+                    "type": "string",
+                    "description": "Directory to keep pipeline Nextflow logs and reports.",
+                    "default": "${params.outdir}/pipeline_info",
+                    "fa_icon": "fas fa-cogs",
+                    "hidden": true
+                }
+            }
+        },
+        "max_job_request_options": {
+            "title": "Max job request options",
+            "type": "object",
+            "fa_icon": "fab fa-acquisitions-incorporated",
+            "description": "Set the top limit for requested resources for any single job.",
+            "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.",
+            "properties": {
+                "max_cpus": {
+                    "type": "integer",
+                    "description": "Maximum number of CPUs that can be requested    for any single job.",
+                    "default": 16,
+                    "fa_icon": "fas fa-microchip",
+                    "hidden": true,
+                    "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`"
+                },
+                "max_memory": {
+                    "type": "string",
+                    "description": "Maximum amount of memory that can be requested for any single job.",
+                    "default": "128.GB",
+                    "fa_icon": "fas fa-memory",
+                    "hidden": true,
+                    "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`"
+                },
+                "max_time": {
+                    "type": "string",
+                    "description": "Maximum amount of time that can be requested for any single job.",
+                    "default": "240.h",
+                    "fa_icon": "far fa-clock",
+                    "hidden": true,
+                    "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`"
+                }
+            }
+        },
+        "institutional_config_options": {
+            "title": "Institutional config options",
+            "type": "object",
+            "fa_icon": "fas fa-university",
+            "description": "Parameters used to describe centralised config profiles. These should not be edited.",
+            "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.",
+            "properties": {
+                "custom_config_version": {
+                    "type": "string",
+                    "description": "Git commit id for Institutional configs.",
+                    "default": "master",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog",
+                    "help_text": "Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.\n\n```bash\n## Download and use config file with following git commit id\n--custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96\n```"
+                },
+                "custom_config_base": {
+                    "type": "string",
+                    "description": "Base directory for Institutional configs.",
+                    "default": "https://raw.githubusercontent.com/nf-core/configs/master",
+                    "hidden": true,
+                    "help_text": "If you're running offline, nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell nextflow where to find them with the `custom_config_base` option. For example:\n\n```bash\n## Download and unzip the config files\ncd /path/to/my/configs\nwget https://github.com/nf-core/configs/archive/master.zip\nunzip master.zip\n\n## Run the pipeline\ncd /path/to/my/data\nnextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/\n```\n\n> Note that the nf-core/tools helper package has a `download` command to download all required pipeline files + singularity containers + institutional configs in one go for you, to make this process easier.",
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "hostnames": {
+                    "type": "string",
+                    "description": "Institutional configs hostname.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "config_profile_description": {
+                    "type": "string",
+                    "description": "Institutional config description.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "config_profile_contact": {
+                    "type": "string",
+                    "description": "Institutional config contact information.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "config_profile_url": {
+                    "type": "string",
+                    "description": "Institutional config URL link.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                }
+            }
+        }
+    },
+    "allOf": [
+        {
+            "$ref": "#/definitions/input_output_options"
+        },
+        {
+            "$ref": "#/definitions/reference_genome_options"
+        },
+        {
+            "$ref": "#/definitions/generic_options"
+        },
+        {
+            "$ref": "#/definitions/max_job_request_options"
+        },
+        {
+            "$ref": "#/definitions/institutional_config_options"
+        }
+    ]
+}

From 61ea7f7f469811e4adc3c7844ef270316cb48756 Mon Sep 17 00:00:00 2001
From: brucemoran <bruce.moran@gmail.com>
Date: Fri, 31 Jul 2020 10:13:04 +0100
Subject: [PATCH 003/359] Suggested changes

---
 docs/output.md         |  4 +++-
 docs/usage.md          |  6 ++++--
 download-references.nf | 26 +++++++++-----------------
 main.nf                |  2 +-
 4 files changed, 17 insertions(+), 21 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 436ef45d..ec1be97b 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -155,7 +155,9 @@ For more info and help check [wiki page](https://github.com/FusionInspector/Fusi
   - filtered list of found fusions (uses tool cutoff as filter, by default: 2, can be adjusted by adding `-t <num>` when running the tool)
 - `fusions_list.txt`
   - unfiltered list of found fusions
-  
+- `<READS_BASE_NAME>_fusion-report.tar.gz`
+  - tarball of the above files to allow easier import to local machine(s) for visualisation through browser
+
 ### Tool detection
 
 Graphs displaying ratio of fusion genes caught by different tools. The last part *all tools* is an intersection of all tools.
diff --git a/docs/usage.md b/docs/usage.md
index 594c56d7..6f33b700 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -226,7 +226,8 @@ If enabled, executes `Ericscript` tool.
 
 ### `--fusioncatcher`
 
-If enabled, executes `Fusioncatcher` tool. N.B. that Fusioncatcher is not available when using the `GRCh37` genome assembly.
+If enabled, executes `Fusioncatcher` tool.
+> N.B. that Fusioncatcher is not available when using the `GRCh37` genome assembly.
 
 - `--fusioncatcher_opt`
   - Specify additional parameters. For more info, please refer to the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md) of the tool.
@@ -308,7 +309,8 @@ Required reference in order to run `Fusioncatcher`.
 
 ### `--genome`
 
-This pipeline uses `Homo Sapiens` version `GRCh38` by default. Assembly `GRCh37` is optionally available. N.B. that using `GRCh37` precludes use of the `Fusioncatcher` tool. Also make sure to specify `--genomes_base`.
+This pipeline uses `Homo Sapiens` version `GRCh38` by default. Assembly `GRCh37` is optionally available.
+> N.B. that using `GRCh37` precludes use of the `Fusioncatcher` tool. Also make sure to specify `--genomes_base`.
 
 ```bash
 --genome 'GRCh38' --genome_base '/path/to/references'
diff --git a/download-references.nf b/download-references.nf
index 9e2d62fe..4dd34b66 100644
--- a/download-references.nf
+++ b/download-references.nf
@@ -98,18 +98,14 @@ process download_base {
     file "Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz" into transcript
 
     script:
+    def base_url = params.genome == "GRCh37" ? "ftp://ftp.ensembl.org/pub/grch37" : "ftp://ftp.ensembl.org/pub/"
     """
-    if [[ ${params.genome} == "GRCh37" ]];then
-      BASEURL="ftp://ftp.ensembl.org/pub/grch37"
-    else
-      BASEURL="ftp://ftp.ensembl.org/pub"
-    fi
-    wget \$BASEURL/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
-    wget \$BASEURL/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
+    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
+    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
     gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}_r${params.reference_release}.all.fa
-    wget \$BASEURL/release-${params.reference_release}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.*.chr.gtf.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
+    wget ${base_url}/release-${params.reference_release}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${params.reference_release}.chr.gtf.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
     gunzip Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
-    wget \$BASEURL/release-${params.reference_release}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz
+    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz
     """
 }
 
@@ -186,23 +182,19 @@ process download_ericscript {
     file '*'
 
     script:
+    def base_url = params.genome == "GRCh37" ? "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIibDRIb0RFdHFlQmM" : "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIiUGt1SnFMZFg4TlE"
     """
-    wget -N https://raw.githubusercontent.com/circulosmeos/gdown.pl/dfd6dc910a38a42d550397bb5c2335be2c4bcf54/gdown.pl
-    chmod +x gdown.pl
     if [[ ${params.genome} == "GRCh37" ]]; then
-      ./gdown.pl "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIibDRIb0RFdHFlQmM" ericscript_db_homosapiens_ensembl73.tar.bz2
-      tar jxf ericscript_db_homosapiens_ensembl73.tar.bz2
+      gdown ${base_url} ericscript_db_homosapiens_ensembl73.tar.bz2
     else
-      ./gdown.pl "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIiUGt1SnFMZFg4TlE" ericscript_db_homosapiens_ensembl84.tar.bz2
-      tar jxf ericscript_db_homosapiens_ensembl84.tar.bz2
+      gdown ${base_url} ericscript_db_homosapiens_ensembl84.tar.bz2
     fi
-    rm gdown.pl ericscript_db_homosapiens_ensembl*.tar.bz2
+    tar jxf ericscript_db_homosapiens_ensembl*.tar.bz2 && rm ericscript_db_homosapiens_ensembl*.tar.bz2
     """
 }
 
 process download_databases {
     publishDir "${params.outdir}/databases", mode: 'copy'
-    executor 'local'
 
     when:
     params.fusion_report || params.download_all
diff --git a/main.nf b/main.nf
index 7d9f46aa..ad5e39b2 100644
--- a/main.nf
+++ b/main.nf
@@ -691,7 +691,7 @@ process arriba_visualization {
     def suff_mem = ("${(task.memory.toBytes() - 6000000000) / task.cpus}" > 2000000000) ? 'true' : 'false'
     def avail_mem = (task.memory && suff_mem) ? "-m" + "${(task.memory.toBytes() - 6000000000) / task.cpus}" : ''
     cytobands = "${reference}/cytobands*${params.genome}*.tsv"
-    proteinDomains = "${reference}/protein_domains*${params.genome}*gff3"
+    proteinDomains = "${reference}/protein_domains*${params.genome}*.gff3"
     """
     samtools sort -@ ${task.cpus} ${avail_mem} -O bam ${bam} > Aligned.sortedByCoord.out.bam
     samtools index Aligned.sortedByCoord.out.bam

From 4e9c3fd9bf97d1440233eb50d8e71e92485f5032 Mon Sep 17 00:00:00 2001
From: brucemoran <bruce.moran@gmail.com>
Date: Fri, 31 Jul 2020 10:31:04 +0100
Subject: [PATCH 004/359] Add gdown to conda env

---
 environment.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/environment.yml b/environment.yml
index 645b707a..8aa9d651 100644
--- a/environment.yml
+++ b/environment.yml
@@ -11,6 +11,7 @@ dependencies:
   - conda-forge::pymdown-extensions=6.0
   - conda-forge::pygments=2.5.2
   # Necessary tools
+  - conda-forge::gdown=3.12.0
   - bioconda::fastqc=0.11.8
   - bioconda::multiqc=1.7
   # Custom packages

From 504e037418ea0ed39e459a884120848eac0fcaeb Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Fri, 31 Jul 2020 13:25:07 +0200
Subject: [PATCH 005/359] Template update for nf-core/tools version 1.10.2

---
 .github/CONTRIBUTING.md              |  2 +-
 .github/workflows/awsfulltest.yml    |  2 +-
 .github/workflows/branch.yml         |  1 +
 .github/workflows/linting.yml        |  9 ++++-
 .github/workflows/push_dockerhub.yml | 53 ++++++++++++++--------------
 Dockerfile                           |  2 +-
 conf/test_full.config                |  2 +-
 docs/usage.md                        |  2 +-
 main.nf                              |  2 +-
 9 files changed, 42 insertions(+), 33 deletions(-)

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index e97e0285..e73e69c7 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -46,7 +46,7 @@ These tests are run both with the latest available version of `Nextflow` and als
 
 ## Patch
 
-: warning: Only in the unlikely and regretful event of a release happening with a bug.
+:warning: Only in the unlikely and regretful event of a release happening with a bug.
 
 * On your own fork, make a new branch `patch` based on `upstream/master`.
 * Fix the bug, and bump version (X.Y.Z+1).
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index f486dc8b..106c1db0 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -21,7 +21,7 @@ jobs:
         run: conda install -c conda-forge awscli
       - name: Start AWS batch job
         # TODO nf-core: You can customise AWS full pipeline tests as required
-        # Add full size test data (but still relatively small datasets for few samples) 
+        # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
         # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command
         env:
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 033f5230..eda35177 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -17,6 +17,7 @@ jobs:
 
 
       # If the above check failed, post a comment on the PR explaining the failure
+      # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
       - name: Post PR comment
         if: failure()
         uses: mshick/add-pr-comment@v1
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index eb66c144..8e8d5bbc 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -57,5 +57,12 @@ jobs:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
-        run: nf-core lint ${GITHUB_WORKSPACE}
+        run: nf-core -l lint_log.txt lint ${GITHUB_WORKSPACE}
+
+      - name: Upload linting log file artifact
+        if: ${{ always() }}
+        uses: actions/upload-artifact@v2
+        with:
+          name: linting-log-file
+          path: lint_log.txt
 
diff --git a/.github/workflows/push_dockerhub.yml b/.github/workflows/push_dockerhub.yml
index a11ca9ad..44440ece 100644
--- a/.github/workflows/push_dockerhub.yml
+++ b/.github/workflows/push_dockerhub.yml
@@ -8,32 +8,33 @@ on:
   release:
     types: [published]
 
-push_dockerhub:
-  name: Push new Docker image to Docker Hub
-  runs-on: ubuntu-latest
-  # Only run for the nf-core repo, for releases and merged PRs
-  if: ${{ github.repository == 'nf-core/rnafusion' }}
-  env:
-    DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }}
-    DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }}
-  steps:
-    - name: Check out pipeline code
-      uses: actions/checkout@v2
+jobs:
+  push_dockerhub:
+    name: Push new Docker image to Docker Hub
+    runs-on: ubuntu-latest
+    # Only run for the nf-core repo, for releases and merged PRs
+    if: ${{ github.repository == 'nf-core/rnafusion' }}
+    env:
+      DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }}
+      DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }}
+    steps:
+      - name: Check out pipeline code
+        uses: actions/checkout@v2
 
-    - name: Build new docker image
-      run: docker build --no-cache . -t nfcore/rnafusion:latest
+      - name: Build new docker image
+        run: docker build --no-cache . -t nfcore/rnafusion:latest
 
-    - name: Push Docker image to DockerHub (dev)
-      if: ${{ github.event_name == 'push' }}
-      run: |
-        echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
-        docker tag nfcore/rnafusion:latest nfcore/rnafusion:dev
-        docker push nfcore/rnafusion:dev
+      - name: Push Docker image to DockerHub (dev)
+        if: ${{ github.event_name == 'push' }}
+        run: |
+          echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
+          docker tag nfcore/rnafusion:latest nfcore/rnafusion:dev
+          docker push nfcore/rnafusion:dev
 
-    - name: Push Docker image to DockerHub (release)
-      if: ${{ github.event_name == 'release' }}
-      run: |
-        echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
-        docker push nfcore/rnafusion:latest
-        docker tag nfcore/rnafusion:latest nfcore/rnafusion:${{ github.event.release.tag_name }}
-        docker push nfcore/rnafusion:${{ github.event.release.tag_name }}
+      - name: Push Docker image to DockerHub (release)
+        if: ${{ github.event_name == 'release' }}
+        run: |
+          echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
+          docker push nfcore/rnafusion:latest
+          docker tag nfcore/rnafusion:latest nfcore/rnafusion:${{ github.event.release.tag_name }}
+          docker push nfcore/rnafusion:${{ github.event.release.tag_name }}
diff --git a/Dockerfile b/Dockerfile
index 95e5d5f9..d5c21ebd 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.10.1
+FROM nfcore/base:1.10.2
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 
diff --git a/conf/test_full.config b/conf/test_full.config
index 96b058e0..64a344d4 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -15,7 +15,7 @@ params {
   // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA)
   // TODO nf-core: Give any required params for the test so that command line flags are not needed
   single_end = false
-  readPaths = [
+  input_paths = [
     ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']],
     ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']]
   ]
diff --git a/docs/usage.md b/docs/usage.md
index 40c74e70..0c4db52b 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -80,7 +80,7 @@ You can also supply a run name to resume a specific run: `-resume [run-name]`. U
 
 ### `-c`
 
-Specify the path to a specific config file (this is a core NextFlow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information.
+Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information.
 
 #### Custom resource requests
 
diff --git a/main.nf b/main.nf
index bd2a1b35..5d283978 100644
--- a/main.nf
+++ b/main.nf
@@ -127,7 +127,7 @@ def summary = [:]
 if (workflow.revision) summary['Pipeline Release'] = workflow.revision
 summary['Run Name']         = custom_runName ?: workflow.runName
 // TODO nf-core: Report custom parameters here
-summary['Reads']            = params.input
+summary['Input']            = params.input
 summary['Fasta Ref']        = params.fasta
 summary['Data Type']        = params.single_end ? 'Single-End' : 'Paired-End'
 summary['Max Resources']    = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job"

From f510a3385dd69e089b7c75ed07facf4c04b5f246 Mon Sep 17 00:00:00 2001
From: brucemoran <bruce.moran@gmail.com>
Date: Sat, 1 Aug 2020 21:55:15 +0100
Subject: [PATCH 006/359] Add GRCh37 to build-ctat.nf, update references.md to
 reflect this option to use Ensembl annotation with STAR-Fusion

---
 build-ctat.nf      | 14 ++++++++------
 docs/references.md |  7 +++++++
 2 files changed, 15 insertions(+), 6 deletions(-)

diff --git a/build-ctat.nf b/build-ctat.nf
index b8b46f78..6f5dfa78 100644
--- a/build-ctat.nf
+++ b/build-ctat.nf
@@ -29,6 +29,7 @@ def helpMessage() {
     Mandatory arguments:
       --fasta [file]                Path to fasta reference
       --gtf [file]                  Path to GTF annotation
+      --genome [str]                Either "GRCh37" or "GRCh38" (default)
       --outdir [path]               Output directory for downloading
       -profile [str]                Configuration profile [https://github.com/nf-core/configs]
     """.stripIndent()
@@ -73,22 +74,23 @@ checkHostname()
 process star_fusion {
     label 'process_high'
     label 'process_long'
-    publishDir "${params.outdir}", mode: 'copy'
+    publishDir "${params.outdir}/star-fusion", mode: 'move'
 
     input:
     file(fasta) from ch_fasta
     file(gtf) from ch_gtf
 
     output:
-    file '*'
+    file 'ctat_genome_lib_build_dir/'
 
     script:
+    def ctat_url = params.genome == "GRCh37" ? "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/CTAT_HumanFusionLib.v0.1.0/CTAT_HumanFusionLib.v0.1.0.dat.gz" : "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.2.0/fusion_lib.Mar2019.dat.gz"
     """
-    wget -N ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_releases/Pfam-A.hmm.gz
+    wget -N ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pfam-A.hmm.gz
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
 
-    wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.2.0/fusion_lib.Mar2019.dat.gz -O CTAT_HumanFusionLib.dat.gz
-    
+    wget ${ctat_url} -O CTAT_HumanFusionLib.dat.gz
+
     # Dfam
     wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm
     wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f
@@ -100,7 +102,7 @@ process star_fusion {
     prep_genome_lib.pl \\
         --genome_fa ${fasta} \\
         --gtf ${gtf} \\
-        --annot_filter_rule /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/ctat-genome-lib-builder-2830cd708c5bb9353878ca98069427e83acdd625/AnnotFilterRuleLib/AnnotFilterRule.pm \\
+        --annot_filter_rule /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/lib/STAR-Fusion/ctat-genome-lib-builder/AnnotFilterRuleLib/AnnotFilterRule.pm \\
         --fusion_annot_lib CTAT_HumanFusionLib.dat.gz \\
         --pfam_db Pfam-A.hmm \\
         --dfam_db homo_sapiens_dfam.hmm \\
diff --git a/docs/references.md b/docs/references.md
index 23a4b8a9..840dc1b7 100644
--- a/docs/references.md
+++ b/docs/references.md
@@ -36,6 +36,13 @@ nextflow run nf-core/rnafusion/download-references.nf \
   --download_all \
   --outdir <PATH> \
   --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+
+# Please note that using the above example command downloads NCBI-based references for STAR-Fusion. To use Ensembl-based references run the following command with the same <PATH> as used above
+nextflow run nf-core/rnafusion/build-ctat.nf \
+  --genome GRCh37 \
+  --outdir <PATH> \
+  --fasta <PATH>/Homo_sapiens.GRCh37_r97.all.fa \
+  --gtf <PATH>/Homo_sapiens.GRCh37_r97.gtf
 ```
 
 ## Tool reference requirements

From 51fcf75d86ad086c69a4e7ba902b5792b1c7d8c0 Mon Sep 17 00:00:00 2001
From: MaxUlysse <max.u.garcia@gmail.com>
Date: Fri, 14 Aug 2020 13:08:28 +0200
Subject: [PATCH 007/359] fix linting

---
 Dockerfile           |   4 +-
 docs/usage.md        |   4 +-
 environment.yml      |   2 +-
 nextflow.config      |   4 +-
 nextflow_schema.json | 145 +++++++++++++++++++++++++++++++++++++++----
 5 files changed, 141 insertions(+), 18 deletions(-)

diff --git a/Dockerfile b/Dockerfile
index d5c21ebd..c380e798 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -7,10 +7,10 @@ COPY environment.yml /
 RUN conda env create --quiet -f /environment.yml && conda clean -a
 
 # Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-1.2.0/bin:$PATH
+ENV PATH /opt/conda/envs/nf-core-rnafusion-1.3.0dev/bin:$PATH
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-1.2.0 > nf-core-rnafusion-1.2.0.yml
+RUN conda env export --name nf-core-rnafusion-1.3.0dev > nf-core-rnafusion-1.3.0dev.yml
 
 # Instruct R processes to use these empty files instead of clashing with a local version
 RUN touch .Rprofile
diff --git a/docs/usage.md b/docs/usage.md
index 5cdfd6af..91f6d4b9 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -520,4 +520,6 @@ NXF_OPTS='-Xms1g -Xmx4g'
 
 ### Automatic resubmission
 
-Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped.
\ No newline at end of file
+Each step in the pipeline has a default set of requirements for number of CPUs, memory and time.
+For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original).
+If it still fails after three times then the pipeline is stopped.
diff --git a/environment.yml b/environment.yml
index 8aa9d651..4f754e10 100644
--- a/environment.yml
+++ b/environment.yml
@@ -1,6 +1,6 @@
 # You can use this file to create a conda environment for this pipeline:
 #   conda env create -f environment.yml
-name: nf-core-rnafusion-1.2.0
+name: nf-core-rnafusion-1.3.0dev
 channels:
   - conda-forge
   - bioconda
diff --git a/nextflow.config b/nextflow.config
index 028f2bb5..04a2092c 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -88,7 +88,7 @@ params {
 
 // Container slug. Stable releases should specify release tag!
 // Developmental code should specify :dev
-process.container = 'nfcore/rnafusion:1.2.0'
+process.container = 'nfcore/rnafusion:dev'
 
 // Load nf-core custom profiles from different Institutions
 try {
@@ -162,7 +162,7 @@ manifest {
   description = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
   mainScript = 'main.nf'
   nextflowVersion = '>=19.10.0'
-  version = '1.2.0'
+  version = '1.3.0dev'
 }
 
 // Function to ensure that resource requirements don't go beyond
diff --git a/nextflow_schema.json b/nextflow_schema.json
index abf782b6..a87cf4ad 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -59,19 +59,39 @@
                     "description": "Path to FASTA genome file.",
                     "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible."
                 },
-                "igenomes_base": {
-                    "type": "string",
-                    "description": "Directory / URL base for iGenomes references.",
-                    "default": "s3://ngi-igenomes/igenomes/",
-                    "fa_icon": "fas fa-cloud-download-alt",
-                    "hidden": true
+                "gtf": {
+                    "type": "string"
                 },
-                "igenomes_ignore": {
-                    "type": "boolean",
-                    "description": "Do not load the iGenomes reference config.",
-                    "fa_icon": "fas fa-ban",
-                    "hidden": true,
-                    "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
+                "genomes_base": {
+                    "type": "string"
+                },
+                "reference_release": {
+                    "type": "integer",
+                    "default": 97
+                },
+                "star_index": {
+                    "type": "string"
+                },
+                "transcript": {
+                    "type": "string"
+                },
+                "base": {
+                    "type": "string"
+                },
+                "arriba_ref": {
+                    "type": "string"
+                },
+                "databases": {
+                    "type": "string"
+                },
+                "ericscript_ref": {
+                    "type": "string"
+                },
+                "fusioncatcher_ref": {
+                    "type": "string"
+                },
+                "star_fusion_ref": {
+                    "type": "string"
                 }
             }
         },
@@ -156,6 +176,90 @@
                 }
             }
         },
+        "new_group_1": {
+            "title": "New Group 1",
+            "type": "object",
+            "description": "",
+            "default": "",
+            "properties": {
+                "arriba": {
+                    "type": "string"
+                },
+                "read_length": {
+                    "type": "integer",
+                    "default": 100
+                },
+                "star_fusion": {
+                    "type": "string"
+                },
+                "fusioncatcher": {
+                    "type": "string"
+                },
+                "fusion_inspector": {
+                    "type": "string"
+                },
+                "ericscript": {
+                    "type": "string"
+                },
+                "pizzly": {
+                    "type": "string"
+                },
+                "squid": {
+                    "type": "string"
+                },
+                "arriba_opt": {
+                    "type": "string"
+                },
+                "arriba_vis": {
+                    "type": "string"
+                },
+                "star_fusion_opt": {
+                    "type": "string"
+                },
+                "fusioncatcher_opt": {
+                    "type": "string"
+                },
+                "pizzly_k": {
+                    "type": "integer",
+                    "default": 31
+                },
+                "fusion_inspector_opt": {
+                    "type": "string"
+                },
+                "fusion_report_opt": {
+                    "type": "string"
+                },
+                "fusion_report": {
+                    "type": "string"
+                },
+                "input_paths": {
+                    "type": "string",
+                    "default": "null",
+                    "hidden": true
+                },
+                "debug": {
+                    "type": "string",
+                    "hidden": true
+                }
+            }
+        },
+        "new_group_2": {
+            "title": "New Group 2",
+            "type": "object",
+            "description": "",
+            "default": "",
+            "properties": {
+                "download_all": {
+                    "type": "string"
+                },
+                "cosmic_usr": {
+                    "type": "string"
+                },
+                "cosmic_passwd": {
+                    "type": "string"
+                }
+            }
+        },
         "max_job_request_options": {
             "title": "Max job request options",
             "type": "object",
@@ -235,6 +339,17 @@
                     "description": "Institutional config URL link.",
                     "hidden": true,
                     "fa_icon": "fas fa-users-cog"
+                },
+                "clusterOptions": {
+                    "type": "string",
+                    "hidden": true
+                },
+                "awsqueue": {
+                    "type": "string"
+                },
+                "awsregion": {
+                    "type": "string",
+                    "default": "eu-west-1"
                 }
             }
         }
@@ -249,6 +364,12 @@
         {
             "$ref": "#/definitions/generic_options"
         },
+        {
+            "$ref": "#/definitions/new_group_1"
+        },
+        {
+            "$ref": "#/definitions/new_group_2"
+        },
         {
             "$ref": "#/definitions/max_job_request_options"
         },

From 648364f6d2bd8057342572c65b1cd452697d022f Mon Sep 17 00:00:00 2001
From: Maxime Garcia <max.u.garcia@gmail.com>
Date: Fri, 14 Aug 2020 13:17:29 +0200
Subject: [PATCH 008/359] Apply suggestions from code review

---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 5eafec2d..1e25a03e 100644
--- a/README.md
+++ b/README.md
@@ -33,7 +33,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 
 1. Install [`nextflow`](https://nf-co.re/usage/installation)
 
-2. Install either [`Docker`](https://docs.docker.com/engine/installation/) or [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) for full pipeline reproducibility; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
+2. Install either [`Docker`](https://docs.docker.com/engine/installation/) or [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) for full pipeline reproducibility; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))
 
 3. Download references for all tools
 

From b71c9b52203c724295697b35bf8849a74fd365f7 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Tue, 22 Sep 2020 17:18:21 +0200
Subject: [PATCH 009/359] fix: using official STAR-Fusion container

---
 CHANGELOG.md                           |  2 ++
 README.md                              |  4 ++--
 bin/scrape_software_versions.py        |  4 ++--
 build-ctat.nf                          |  4 ++--
 conf/base.config                       | 15 ++++++++-------
 containers/star-fusion/Dockerfile      | 20 --------------------
 containers/star-fusion/environment.yml | 12 ------------
 main.nf                                |  4 ++--
 8 files changed, 18 insertions(+), 47 deletions(-)
 delete mode 100644 containers/star-fusion/Dockerfile
 delete mode 100644 containers/star-fusion/environment.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e9060a4b..5c0f0bc8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -7,6 +7,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## dev nfcore/rnafusion - 2020/07/15
 
+* Using official STAR-Fusion container [#160](https://github.com/nf-core/rnafusion/issues/160)
+
 ### Added
 
 * Added social preview image [#107](https://github.com/nf-core/rnafusion/issues/107)
diff --git a/README.md b/README.md
index 1e25a03e..8e56e62d 100644
--- a/README.md
+++ b/README.md
@@ -23,11 +23,11 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 | [Arriba](https://github.com/suhrig/arriba)                                |         :x:        |  `1.2.0` |
 | [EricScript](https://sites.google.com/site/bioericscript/getting-started) |         :x:        |  `0.5.5` |
 | [FusionCatcher](https://github.com/ndaniel/fusioncatcher)                 | :white_check_mark: |  `1.20`  |
-| [Fusion-Inspector](https://github.com/FusionInspector/FusionInspector)    |         :x:        |  `2.2.1` |
+| [Fusion-Inspector](https://github.com/FusionInspector/FusionInspector)    |         :x:        |  `2.3.1` |
 | [fusion-report](https://github.com/matq007/fusion-report)                 |          -         |  `2.1.3` |
 | [Pizzly](https://github.com/pmelsted/pizzly)                              |         :x:        | `0.37.3` |
 | [Squid](https://github.com/Kingsford-Group/squid)                         |         :x:        |   `1.5`  |
-| [Star-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: |  `1.8.1` |
+| [Star-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: |  `1.9.1` |
 
 ## Quick Start
 
diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
index 1618db14..f949eef2 100755
--- a/bin/scrape_software_versions.py
+++ b/bin/scrape_software_versions.py
@@ -12,10 +12,10 @@
     "Arriba": ["v_arriba.txt", r"arriba=(\S+)"],
     "EricScript": ["v_ericscript.txt", r"ericscript=(\S+)"],
     "FusionCatcher": ["v_fusioncatcher.txt", r"fusioncatcher=(\S+)"],
-    "Fusion-Inspector": ["v_fusion_inspector.txt", r"fusion-inspector=(\S+)"],
+    "Fusion-Inspector": ["v_fusion_inspector.txt", r"fusion_inspector = (\S+)"],
     "fusion-report": ["v_fusion_report.txt", r"fusion-report=(\S+)"],
     "Pizzly": ["v_pizzly.txt", r"pizzly=(\S+)"],
-    "STAR-Fusion": ["v_star_fusion.txt", r"star-fusion=(\S+)"],
+    "STAR-Fusion": ["v_star_fusion.txt", r"star_fusion = (\S+)"],
     "Squid": ["v_squid.txt", r"squid=(\S+)"]
 }
 results = OrderedDict()
diff --git a/build-ctat.nf b/build-ctat.nf
index 6f5dfa78..8bb0fbc2 100644
--- a/build-ctat.nf
+++ b/build-ctat.nf
@@ -99,10 +99,10 @@ process star_fusion {
     wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p
 
     export TMPDIR=/tmp
-    prep_genome_lib.pl \\
+    /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl \\
         --genome_fa ${fasta} \\
         --gtf ${gtf} \\
-        --annot_filter_rule /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/lib/STAR-Fusion/ctat-genome-lib-builder/AnnotFilterRuleLib/AnnotFilterRule.pm \\
+        --annot_filter_rule /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/AnnotFilterRuleLib/AnnotFilterRule.pm \\
         --fusion_annot_lib CTAT_HumanFusionLib.dat.gz \\
         --pfam_db Pfam-A.hmm \\
         --dfam_db homo_sapiens_dfam.hmm \\
diff --git a/conf/base.config b/conf/base.config
index 0081fde2..c6d26d72 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -11,12 +11,13 @@
 
 params {
     versions {
-    arriba = '1.2.0'
-    ericscript = '0.5.5'
-    fusioncatcher = '1.20'
-    pizzly = '0.37.3'
-    squid = '1.5-star2.7.1a'
-    star_fusion = '1.8.1'
+      arriba = '1.2.0'
+      ericscript = '0.5.5'
+      fusioncatcher = '1.20'
+      pizzly = '0.37.3'
+      squid = '1.5-star2.7.1a'
+      star_fusion = '1.9.1'
+      fusion_inspector = '2.3.1'
   }
 }
 
@@ -68,6 +69,6 @@ process {
     container = "nfcore/rnafusion:squid_${params.versions.squid}"
   }
   withName:"star_fusion|fusion_inspector" {
-    container = "nfcore/rnafusion:star-fusion_${params.versions.star_fusion}"
+    container = "trinityctat/starfusion:${params.versions.star_fusion}"
   }
 }
\ No newline at end of file
diff --git a/containers/star-fusion/Dockerfile b/containers/star-fusion/Dockerfile
deleted file mode 100644
index 79c6fdbc..00000000
--- a/containers/star-fusion/Dockerfile
+++ /dev/null
@@ -1,20 +0,0 @@
-FROM nfcore/base:1.9
-
-LABEL authors="Martin Proks" \
-    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
-
-# Install the conda environment
-COPY environment.yml /
-RUN conda env create -f /environment.yml && conda clean -a
-
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/bin:$PATH
-
-# FusionInspector
-ENV PATH /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/lib/STAR-Fusion/FusionInspector:$PATH
-
-# ctat-genome-lib-builder
-ENV PATH /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/lib/STAR-Fusion/ctat-genome-lib-builder:$PATH
-
-# Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-star-fusion_1.8.1 > nf-core-rnafusion-star-fusion_1.8.1.yml
\ No newline at end of file
diff --git a/containers/star-fusion/environment.yml b/containers/star-fusion/environment.yml
deleted file mode 100644
index 4ca4be91..00000000
--- a/containers/star-fusion/environment.yml
+++ /dev/null
@@ -1,12 +0,0 @@
-name: nf-core-rnafusion-star-fusion_1.8.1
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::dfam=2.0
-  - bioconda::hmmer=3.2.1
-  - bioconda::star-fusion=1.8.1
-  - bioconda::trinity=2.6.6
-  - bioconda::samtools=1.9
-  - conda-forge::perl-carp-assert
\ No newline at end of file
diff --git a/main.nf b/main.nf
index cf15754a..a4c4c754 100644
--- a/main.nf
+++ b/main.nf
@@ -773,8 +773,8 @@ process get_software_versions {
     multiqc --version > v_multiqc.txt
     cat ${baseDir}/containers/arriba/environment.yml > v_arriba.txt
     cat ${baseDir}/containers/fusioncatcher/environment.yml > v_fusioncatcher.txt
-    cat ${baseDir}/containers/star-fusion/environment.yml > v_fusion_inspector.txt
-    cat ${baseDir}/containers/star-fusion/environment.yml > v_star_fusion.txt
+    cat ${baseDir}/conf/base.config | grep -m1 fusion_inspector > v_fusion_inspector.txt
+    cat ${baseDir}/conf/base.config | grep -m1 star_fusion > v_star_fusion.txt
     cat ${baseDir}/containers/ericscript/environment.yml > v_ericscript.txt
     cat ${baseDir}/containers/pizzly/environment.yml > v_pizzly.txt
     cat ${baseDir}/containers/squid/environment.yml > v_squid.txt

From 94fa3ea4424486396fb55a3f7de09feb4a1b404e Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Fri, 25 Sep 2020 12:47:54 +0200
Subject: [PATCH 010/359] fix: homo_sapiens_dfam requires
 `--no-check-certificate`

---
 build-ctat.nf | 12 ++++++------
 1 file changed, 6 insertions(+), 6 deletions(-)

diff --git a/build-ctat.nf b/build-ctat.nf
index 8bb0fbc2..272e9e83 100644
--- a/build-ctat.nf
+++ b/build-ctat.nf
@@ -89,14 +89,14 @@ process star_fusion {
     wget -N ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pfam-A.hmm.gz
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
 
-    wget ${ctat_url} -O CTAT_HumanFusionLib.dat.gz
+    wget -N ${ctat_url} -O CTAT_HumanFusionLib.dat.gz
 
     # Dfam
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p
+    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm
+    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f
+    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i
+    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m
+    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p
 
     export TMPDIR=/tmp
     /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl \\

From ce68f7afa49c7567c3f37aca3fbb570748ddc465 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Fri, 2 Oct 2020 09:56:34 +0200
Subject: [PATCH 011/359] fix: increased horse power for building star-index

---
 main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/main.nf b/main.nf
index a4c4c754..a5a773d5 100644
--- a/main.nf
+++ b/main.nf
@@ -279,7 +279,7 @@ Channel.from(summary.collect{ [it.key, it.value] })
 
 process build_star_index {
     tag "${fasta}-${gtf}"
-    label 'process_medium'
+    label 'process_high'
 
     publishDir params.outdir, mode: 'copy'
 

From f6a052ca021155c17abbca1145f8b7609b2c96cd Mon Sep 17 00:00:00 2001
From: Tobias Neumann <tobias.neumann.at@gmail.com>
Date: Fri, 2 Oct 2020 16:01:51 +0200
Subject: [PATCH 012/359] Capture full fusion_inspector output.

---
 main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/main.nf b/main.nf
index a5a773d5..37168013 100644
--- a/main.nf
+++ b/main.nf
@@ -728,7 +728,7 @@ process fusion_inspector {
         file(reference) from reference.fusion_inspector
 
     output:
-        file("*.{fa,gtf,bed,bam,bai,txt,html}") into fusion_inspector_output
+        file("*.{fa,gtf,bed,bam,bai,txt,html,tsv,gff3,fasta}") into fusion_inspector_output
 
     when: params.fusion_inspector && (!params.single_end || params.debug)
 

From 26125b9661421c668da39794d8545abe0c650079 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Tue, 6 Oct 2020 11:40:44 +0200
Subject: [PATCH 013/359] fix: optional publish_dir mode from TEMPLATE

---
 main.nf | 20 ++++++++++----------
 1 file changed, 10 insertions(+), 10 deletions(-)

diff --git a/main.nf b/main.nf
index 37168013..359e53a9 100644
--- a/main.nf
+++ b/main.nf
@@ -281,7 +281,7 @@ process build_star_index {
     tag "${fasta}-${gtf}"
     label 'process_high'
 
-    publishDir params.outdir, mode: 'copy'
+    publishDir params.outdir, mode: params.publish_dir_mode
 
     input:
         file(fasta) from ch_fasta
@@ -324,7 +324,7 @@ process arriba {
     tag "${sample}"
     label 'process_medium'
 
-    publishDir "${params.outdir}/tools/Arriba/${sample}", mode: 'copy'
+    publishDir "${params.outdir}/tools/Arriba/${sample}", mode: params.publish_dir_mode
 
     input:
         set val(sample), file(reads) from read_files_arriba
@@ -390,7 +390,7 @@ process star_fusion {
     tag "${sample}"
     label 'process_high'
 
-    publishDir "${params.outdir}/tools/Star-Fusion/${sample}", mode: 'copy'
+    publishDir "${params.outdir}/tools/Star-Fusion/${sample}", mode: params.publish_dir_mode
 
     input:
         set val(sample), file(reads) from read_files_star_fusion
@@ -457,7 +457,7 @@ process fusioncatcher {
     tag "${sample}"
     label 'process_high'
 
-    publishDir "${params.outdir}/tools/Fusioncatcher/${sample}", mode: 'copy'
+    publishDir "${params.outdir}/tools/Fusioncatcher/${sample}", mode: params.publish_dir_mode
 
     input:
         set val(sample), file(reads) from read_files_fusioncatcher
@@ -493,7 +493,7 @@ process ericscript {
     tag "${sample}"
     label 'process_high'
 
-    publishDir "${params.outdir}/tools/EricScript/${sample}", mode: 'copy'
+    publishDir "${params.outdir}/tools/EricScript/${sample}", mode: params.publish_dir_mode
 
     input:
         set val(sample), file(reads) from read_files_ericscript
@@ -533,7 +533,7 @@ process pizzly {
     tag "${sample}"
     label 'process_medium'
 
-    publishDir "${params.outdir}/tools/Pizzly/${sample}", mode: 'copy'
+    publishDir "${params.outdir}/tools/Pizzly/${sample}", mode: params.publish_dir_mode
 
     input:
         set val(sample), file(reads) from read_files_pizzly
@@ -575,7 +575,7 @@ process squid {
     tag "${sample}"
     label 'process_high'
 
-    publishDir "${params.outdir}/tools/Squid/${sample}", mode: 'copy'
+    publishDir "${params.outdir}/tools/Squid/${sample}", mode: params.publish_dir_mode
 
     input:
         set val(sample), file(reads) from read_files_squid
@@ -638,7 +638,7 @@ files_and_reports_summary = files_and_reports_summary.dump(tag:'files_and_report
 process summary {
     tag "${sample}"
 
-    publishDir "${params.outdir}/Reports/${sample}", mode: 'copy'
+    publishDir "${params.outdir}/Reports/${sample}", mode: params.publish_dir_mode
 
     input:
         set val(sample), file(reads), file(arriba), file(ericscript), file(fusioncatcher), file(pizzly), file(squid), file(starfusion) from files_and_reports_summary
@@ -679,7 +679,7 @@ process arriba_visualization {
     tag "${sample}"
     label 'process_medium'
 
-    publishDir "${params.outdir}/tools/Arriba/${sample}", mode: 'copy'
+    publishDir "${params.outdir}/tools/Arriba/${sample}", mode: params.publish_dir_mode
 
     input:
         file(reference) from reference.arriba_vis
@@ -721,7 +721,7 @@ process fusion_inspector {
     tag "${sample}"
     label 'process_high'
 
-    publishDir "${params.outdir}/tools/FusionInspector/${sample}", mode: 'copy'
+    publishDir "${params.outdir}/tools/FusionInspector/${sample}", mode: params.publish_dir_mode
 
     input:
         set val(sample), file(fi_input_list), file(reads) from fusion_inspector_input

From 8da3392c488e89841862dcb131b8552a99b6c383 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Tue, 6 Oct 2020 11:42:48 +0200
Subject: [PATCH 014/359] fix: removed docker extra run option parameters

---
 nextflow.config | 5 ++++-
 1 file changed, 4 insertions(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 04a2092c..2f7d2565 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -119,7 +119,10 @@ profiles {
     //   WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap.
     // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351
     // once this is established and works well, nextflow might implement this behavior as new default.
-    docker.runOptions = '-u \$(id -u):\$(id -g)'
+    
+    // Some people are experiencing issues with enforcing this runOptions. If you are experiencing issues, try including this
+    // in your custom configuration.
+    // docker.runOptions = '-u \$(id -u):\$(id -g)'
   }
   singularity {
     singularity.enabled = true

From 0ffb915a03e29f517d130cbce91994c45f954b62 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 27 Oct 2020 09:25:29 +0000
Subject: [PATCH 015/359] Template update for nf-core/tools version 1.11

---
 .github/CONTRIBUTING.md                     |   2 +-
 .github/ISSUE_TEMPLATE/bug_report.md        |   2 +-
 .github/workflows/awsfulltest.yml           |   4 +-
 .github/workflows/awstest.yml               |   9 +-
 .github/workflows/ci.yml                    |  10 +-
 .gitignore                                  |   3 +-
 CHANGELOG.md                                | 154 +---
 Dockerfile                                  |   2 +-
 README.md                                   |  93 +--
 assets/nf-core-rnafusion_social_preview.png | Bin 47745 -> 0 bytes
 assets/nf-core-rnafusion_social_preview.svg | 446 ------------
 bin/scrape_software_versions.py             |  18 +-
 build-ctat.nf                               | 162 -----
 conf/base.config                            |  50 +-
 conf/genomes.config                         |  33 -
 conf/igenomes.config                        | 421 +++++++++++
 conf/test.config                            |  10 +-
 containers/arriba/Dockerfile                |  14 -
 containers/arriba/environment.yml           |  14 -
 containers/ericscript/Dockerfile            |  17 -
 containers/ericscript/environment.yml       |   8 -
 containers/fusioncatcher/Dockerfile         |  14 -
 containers/fusioncatcher/environment.yml    |   7 -
 containers/pizzly/Dockerfile                |  14 -
 containers/pizzly/environment.yml           |   9 -
 containers/squid/Dockerfile                 |  19 -
 containers/squid/environment.yml            |  11 -
 containers/star-fusion/Dockerfile           |  20 -
 containers/star-fusion/environment.yml      |  12 -
 docs/images/BTB_logo.png                    | Bin 13624 -> 0 bytes
 docs/images/BTB_logo.svg                    | 184 -----
 docs/images/NGI_logo.png                    | Bin 20547 -> 0 bytes
 docs/images/NGI_logo.svg                    | 333 ---------
 docs/images/SDU_logo.png                    | Bin 11894 -> 0 bytes
 docs/images/SciLifeLab_logo.png             | Bin 8387 -> 0 bytes
 docs/images/SciLifeLab_logo.svg             |  99 ---
 docs/images/rnafusion_logo.png              | Bin 15692 -> 0 bytes
 docs/images/rnafusion_logo.svg              | 208 ------
 docs/images/summary_graph_1.png             | Bin 95932 -> 0 bytes
 docs/images/summary_graph_2.png             | Bin 66019 -> 0 bytes
 docs/images/summary_graph_3.png             | Bin 83535 -> 0 bytes
 docs/output.md                              | 271 +------
 docs/usage.md                               | 467 +-----------
 download-references.nf                      | 260 -------
 environment.yml                             |  12 +-
 main.nf                                     | 742 ++------------------
 nextflow.config                             |  82 +--
 nextflow_schema.json                        | 149 +---
 scripts/build.sh                            |  51 --
 49 files changed, 659 insertions(+), 3777 deletions(-)
 delete mode 100644 assets/nf-core-rnafusion_social_preview.png
 delete mode 100644 assets/nf-core-rnafusion_social_preview.svg
 delete mode 100644 build-ctat.nf
 delete mode 100644 conf/genomes.config
 create mode 100644 conf/igenomes.config
 delete mode 100644 containers/arriba/Dockerfile
 delete mode 100644 containers/arriba/environment.yml
 delete mode 100644 containers/ericscript/Dockerfile
 delete mode 100644 containers/ericscript/environment.yml
 delete mode 100644 containers/fusioncatcher/Dockerfile
 delete mode 100644 containers/fusioncatcher/environment.yml
 delete mode 100644 containers/pizzly/Dockerfile
 delete mode 100644 containers/pizzly/environment.yml
 delete mode 100644 containers/squid/Dockerfile
 delete mode 100644 containers/squid/environment.yml
 delete mode 100644 containers/star-fusion/Dockerfile
 delete mode 100644 containers/star-fusion/environment.yml
 delete mode 100644 docs/images/BTB_logo.png
 delete mode 100644 docs/images/BTB_logo.svg
 delete mode 100644 docs/images/NGI_logo.png
 delete mode 100644 docs/images/NGI_logo.svg
 delete mode 100644 docs/images/SDU_logo.png
 delete mode 100644 docs/images/SciLifeLab_logo.png
 delete mode 100644 docs/images/SciLifeLab_logo.svg
 delete mode 100644 docs/images/rnafusion_logo.png
 delete mode 100644 docs/images/rnafusion_logo.svg
 delete mode 100644 docs/images/summary_graph_1.png
 delete mode 100644 docs/images/summary_graph_2.png
 delete mode 100644 docs/images/summary_graph_3.png
 delete mode 100644 download-references.nf
 delete mode 100755 scripts/build.sh

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index e73e69c7..7f74910f 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -54,4 +54,4 @@ These tests are run both with the latest available version of `Nextflow` and als
 
 ## Getting help
 
-For further information/help, please consult the [nf-core/rnafusion documentation](https://nf-co.re/rnafusion/docs) and don't hesitate to get in touch on the nf-core Slack [#rnafusion](https://nfcore.slack.com/channels/rnafusion) channel ([join our Slack here](https://nf-co.re/join/slack)).
+For further information/help, please consult the [nf-core/rnafusion documentation](https://nf-co.re/rnafusion/usage) and don't hesitate to get in touch on the nf-core Slack [#rnafusion](https://nfcore.slack.com/channels/rnafusion) channel ([join our Slack here](https://nf-co.re/join/slack)).
diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md
index c9950f88..09c1d9d4 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.md
+++ b/.github/ISSUE_TEMPLATE/bug_report.md
@@ -36,7 +36,7 @@ Steps to reproduce the behaviour:
 
 ## Container engine
 
-- Engine: <!-- [e.g. Conda, Docker or Singularity] -->
+- Engine: <!-- [e.g. Conda, Docker, Singularity or Podman] -->
 - version: <!-- [e.g. 1.0.0] -->
 - Image tag: <!-- [e.g. nfcore/rnafusion:1.0.0] -->
 
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 106c1db0..214d01aa 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -1,10 +1,12 @@
 name: nf-core AWS full size tests
-# This workflow is triggered on push to the master branch.
+# This workflow is triggered on published releases.
+# It can be additionally triggered manually with GitHub actions workflow dispatch.
 # It runs the -profile 'test_full' on AWS batch
 
 on:
   release:
     types: [published]
+  workflow_dispatch:
 
 jobs:
   run-awstest:
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index b687404c..e54f10f7 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -1,11 +1,10 @@
 name: nf-core AWS test
 # This workflow is triggered on push to the master branch.
-# It runs the -profile 'test' on AWS batch
+# It can be additionally triggered manually with GitHub actions workflow dispatch.
+# It runs the -profile 'test' on AWS batch.
 
 on:
-  push:
-    branches:
-      - master
+  workflow_dispatch:
 
 jobs:
   run-awstest:
@@ -37,4 +36,4 @@ jobs:
           --job-name nf-core-rnafusion \
           --job-queue $AWS_JOB_QUEUE \
           --job-definition $AWS_JOB_DEFINITION \
-          --container-overrides '{"command": ["nf-core/rnafusion", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/rnafusion/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/rnafusion/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'rnafusion/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'
+          --container-overrides '{"command": ["nf-core/rnafusion", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/rnafusion/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/rnafusion/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 4991dc7b..abcbc3b0 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -47,9 +47,9 @@ jobs:
           wget -qO- get.nextflow.io | bash
           sudo mv nextflow /usr/local/bin/
 
-      - name: Run pipeline help
+      - name: Run pipeline with test data
+        # TODO nf-core: You can customise CI pipeline run tests as required
+        # For example: adding multiple test runs with different parameters
+        # Remember that you can parallelise this by using strategy.matrix
         run: |
-          nextflow run ${GITHUB_WORKSPACE} --help
-      - name: Run pipeline for downloading references help
-        run: |
-          nextflow run ${GITHUB_WORKSPACE}/download-references.nf --help
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker
diff --git a/.gitignore b/.gitignore
index ed9be7c2..aa4bb5b3 100644
--- a/.gitignore
+++ b/.gitignore
@@ -1,10 +1,9 @@
 .nextflow*
 work/
+data/
 results/
 .DS_Store
-test/
 tests/
 testing/
 testing*
 *.pyc
-.vscode/
\ No newline at end of file
diff --git a/CHANGELOG.md b/CHANGELOG.md
index e9060a4b..01d65559 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -1,156 +1,16 @@
-# nfcore/rnafusion: Changelog
-
-All notable changes to this project will be documented in this file.
+# nf-core/rnafusion: Changelog
 
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## dev nfcore/rnafusion - 2020/07/15
-
-### Added
-
-* Added social preview image [#107](https://github.com/nf-core/rnafusion/issues/107)
-* Added support for GRCh37 genome assembly [#77](https://github.com/nf-core/rnafusion/issues/77)
-
-### Changed
-
-* Upgrade `fusion-report v2.1.2` to `fusion-report v2.1.3`
-* Upgrade `fusion-report v2.1.1` to `fusion-report v2.1.2`
-* Upgrade `fusion-report v2.1.0` to `fusion-report v2.1.1`
-* Upgrade `Arriba v1.1.0` to `Arriba v1.2.0`
-* Upgrade `fusion-report v2.0.2` to `fusion-report v2.1.0`
-
-### Fixed
-
-* Missing `strip-components` in `download-references.nf/star-fusion` [#148](https://github.com/nf-core/rnafusion/issues/148)
-* Missing version prefix for cdna [#143](https://github.com/nf-core/rnafusion/issues/143)
-* `samtools` missing header in empty file for FusionInspector [ref](https://github.com/STAR-Fusion/STAR-Fusion/issues/191)
-* Removed `profile` from helper scripts [#139](https://github.com/nf-core/rnafusion/issues/139)
-* Wrong url path for `Pfam-A.hmm.gz` [#140](https://github.com/nf-core/rnafusion/issues/140)
-
-### Removed
-
-* Removed `scripts/download-singularity-img.sh` and `download-singularity-img.nf` as they are not necessary any more
-
-## [1.1.0] nfcore/rnafusion - 2020/02/10
-
-* Fusion gene detection tools:
-  * `Arriba v1.1.0`
-  * `Ericscript v0.5.5`
-  * `Fusioncatcher v1.20`
-  * `Pizzly v0.37.3`
-  * `Squid v1.5`
-  * `STAR-Fusion v1.6.0`
-* Visualization tools:
-  * `Arriba v1.1.0`
-  * `FusionInspector v1.3.1`
-* Other tools:
-  * `fusion-report v2.0.1`
-  * `FastQ v0.11.8`
-  * `MultiQC v1.7`
-  * `STAR aligner v2.7.0f`
-
-### Added
-
-* Added `Arriba 1.1.0` [#63](https://github.com/nf-core/rnafusion/issues/63)
-* Added Batch mode [#54](https://github.com/nf-core/rnafusion/issues/54)
-
-### Changed
-
-* Updated examples and configurations
-* Upgraded `fusion-report v1.0.0` to `fusion-report v2.0.1`
-* Divided `running_tools` into fusion and visualization tools
-* Updated `STAR` in `Squid`, `Fusion-Inspector` version to `2.7.0f`
-* Upgraded `STAR-Fusion v1.5.0` to `STAR-Fusion v1.6.0` [#83](https://github.com/nf-core/rnafusion/issues/83)
-* Parameter `igenomesIgnore` renamed to `igenome` [#81](https://github.com/nf-core/rnafusion/issues/81)
-* Finished STAR-Fusion file renaming [#18](https://github.com/nf-core/rnafusion/issues/18)
-* Updated logos
-* Updated to nf-core `1.8` TEMPLATE
-
-### Fixed
-
-* iGenomes optional, but not really [#91](https://github.com/nf-core/rnafusion/issues/91)
-* Updated `fusioncatcher` to latest `1.20` version also solving [#95](https://github.com/nf-core/rnafusion/issues/95)
-
-### Removed
-
-* Variables `pizzly_fasta` and `pizzly_gtf` have been removed and replaced with `transcript` and `gtf`
-* `Jenkisfile`, test configuration, pylintrc configuration
-* Removed `igenomes.config` because the pipeline only supports `Ensembl` version
-
----
-
-## [1.0.2] nfcore/rnafusion - 2019/05/13
-
-### Changed
-
-* Bumped nf-core template to 1.6 [#69](https://github.com/nf-core/rnafusion/pull/69)
-
-### Fixed
-
-* Fixed COSMIC parameters not wrapped in quotes [#75](https://github.com/nf-core/rnafusion/issues/75)
-* Implemented output output for fusion tools [#72](https://github.com/nf-core/rnafusion/issues/72)
-* Fixed reference download link for STAR-Fusion [#71](https://github.com/nf-core/rnafusion/issues/71)
-
----
-
-## [1.0.1] nfcore/rnafusion - 2019/04/06
-
-### Added
-
-* Added support for extra parameters for tools STAR-Fusion, FusionCatcher and fusion-report
-* Added example configuration for `singularity` and `docker`
-* Added [fusion-report](https://github.com/matq007/fusion-report) into the stack [#62](https://github.com/nf-core/rnafusion/issues/62), [#55](https://github.com/nf-core/rnafusion/issues/55), [#53](https://github.com/nf-core/rnafusion/issues/53), [#51](https://github.com/nf-core/rnafusion/issues/51)
-* Added nextflow helper script `download-singularity-img.nf`
-* Added nextflow helper script `download-references.nf`
-* Added `Jenkinsfile` for in-house testing
-
-### Changed
-
-* Updated installation of `FusionCatcher` (available now on bioconda)
-
-### Fixed
-
-* Fixed empty symlinks (`input.X`) in fusion-report [#68](https://github.com/nf-core/rnafusion/issues/68)
-* Fixed FASTA issues [#60](https://github.com/nf-core/rnafusion/issues/60)
-* Fixed centralized nf-core/config [#64](https://github.com/nf-core/rnafusion/issues/64)
-* Fixed `scrape_software_versions.py` to parse tools versions correctly [#65](https://github.com/nf-core/rnafusion/issues/65)
-
-### Removed
-
-* Removed `Singularity`
-
----
-
-## [1.0] nfcore/rnafusion - 2018/02/14
-
-Version 1.0 marks the first production release of this pipeline under the nf-core flag.
-The pipeline includes additional help scripts to download references for fusion tools and Singularity images.
+## v1.3.0dev - [date]
 
-* Fusion gene detection tools:
-  * `STAR-Fusion v1.5.0`
-  * `Fusioncatcher v1.00`
-  * `Ericscript v0.5.5`
-  * `Pizzly v0.37.3`
-  * `Squid v1.5`
-* Visualization tools:
-  * `FusionInspector v1.3.1`
-* Other tools:
-  * `Summary report`
-  * `FastQ v0.11.8`
-  * `MultiQC v1.7`
-  * `FusionGDB updated 2019/01/23`
+Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
 
----
+### `Added`
 
-## [0.1] SciLifeLab/NGI-RNAfusion (ARCHIVED) - 2018/10/05
+### `Fixed`
 
-Initial release of NGI-RNAfusion, created with the [nf-core](http://nf-co.re/) template.
-Source code can be found at [SciLifeLab/NGI-RNAfusion](https://github.com/SciLifeLab/NGI-RNAfusion).
-The solution works with Docker and Singularity.
+### `Dependencies`
 
-* Tools:
-  * STAR-Fusion
-  * Fusioncatcher
-  * FusionInspector
-  * Custom tool for fusion comparison - generates intersection of detected fusion genes from all tools
+### `Deprecated`
diff --git a/Dockerfile b/Dockerfile
index c380e798..deb83220 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.10.2
+FROM nfcore/base:1.11
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 
diff --git a/README.md b/README.md
index 1e25a03e..09cf24b9 100644
--- a/README.md
+++ b/README.md
@@ -1,14 +1,12 @@
 # ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo.png)
 
-> RNA sequencing analysis pipeline with curated list of tools for detecting and visualizing fusion genes
-
-[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A519.10.0-brightgreen.svg)](https://www.nextflow.io/)
-[![nf-core](https://img.shields.io/badge/nf--core-pipeline-brightgreen.svg)](https://nf-co.re/)
-[![DOI](https://zenodo.org/badge/151721952.svg)](https://zenodo.org/badge/latestdoi/151721952)
+**Nextflow rnafusion analysis pipeline, part of the nf-core community.**.
 
 [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions)
+[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A519.10.0-brightgreen.svg)](https://www.nextflow.io/)
 
+[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/)
 [![Docker](https://img.shields.io/docker/automated/nfcore/rnafusion.svg)](https://hub.docker.com/r/nfcore/rnafusion)
 [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?logo=slack)](https://nfcore.slack.com/channels/rnafusion)
 
@@ -16,89 +14,39 @@
 
 The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible.
 
-> The pipeline **requires** >=16 CPU cores and >=30GB RAM
-
-| Tool                                                                      |  Single-end reads  |  Version |
-| ------------------------------------------------------------------------- | :----------------: | :------: |
-| [Arriba](https://github.com/suhrig/arriba)                                |         :x:        |  `1.2.0` |
-| [EricScript](https://sites.google.com/site/bioericscript/getting-started) |         :x:        |  `0.5.5` |
-| [FusionCatcher](https://github.com/ndaniel/fusioncatcher)                 | :white_check_mark: |  `1.20`  |
-| [Fusion-Inspector](https://github.com/FusionInspector/FusionInspector)    |         :x:        |  `2.2.1` |
-| [fusion-report](https://github.com/matq007/fusion-report)                 |          -         |  `2.1.3` |
-| [Pizzly](https://github.com/pmelsted/pizzly)                              |         :x:        | `0.37.3` |
-| [Squid](https://github.com/Kingsford-Group/squid)                         |         :x:        |   `1.5`  |
-| [Star-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: |  `1.8.1` |
-
 ## Quick Start
 
 1. Install [`nextflow`](https://nf-co.re/usage/installation)
 
-2. Install either [`Docker`](https://docs.docker.com/engine/installation/) or [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) for full pipeline reproducibility; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))
+2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) or [`Podman`](https://podman.io/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
 
-3. Download references for all tools
+3. Download the pipeline and test it on a minimal dataset with a single command:
 
     ```bash
-    nextflow run nf-core/rnafusion/download-references.nf -profile <docker/singularity/institute> \
-      --download_all \
-      --genome GRCh38 \
-      --outdir <PATH> \
-      --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+    nextflow run nf-core/rnafusion -profile test,<docker/singularity/podman/conda/institute>
     ```
 
-    > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute.
-    > If so, you can simply use `-profile <institute>` in your command.
-    > This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
+    > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
 
 4. Start running your own analysis!
 
-  ```bash
-  nextflow run nf-core/rnafusion -profile <docker/singularity/institute> \
-    --input "*_R{1,2}.fastq.gz" \
-    --genomes_base <reference_path>
-    --arriba --star_fusion --fusioncatcher --ericscript --pizzly --squid \
-    --arriba_vis --fusion_inspector
-  ```
-
-See [usage docs](docs/usage.md) for all of the available options when running the pipeline.
-
-## Documentation
-
-The nf-core/rnafusion pipeline comes with documentation about the pipeline which you can read at [https://nf-core/rnafusion/docs](https://nf-core/rnafusion/docs) or find in the [`docs/` directory](docs).
-
-## Credits
+    <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
 
-Following a development initiated by [Rickard Hammarén](https://github.com/Hammarn), this pipeline was originally developed by [Martin Proks](https://github.com/matq007) developed at the [National Genomics Infastructure](https://ngisweden.scilifelab.se) from [SciLifeLab](https://scilifelab.se), with the support of [The Swedish Childhood Tumor Biobank (Barntumörbanken)](https://ki.se/forskning/barntumorbanken) and [University of Southern Denmark](https://www.sdu.dk/en) as a master thesis.
+    ```bash
+    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/conda/institute> --input '*_R{1,2}.fastq.gz' --genome GRCh37
+    ```
 
-Main authors:
+See [usage docs](https://nf-co.re/rnafusion/usage) for all of the available options when running the pipeline.
 
-* [Martin Proks](https://github.com/matq007)
-* [Maxime Garcia](https://github.com/MaxUlysse)
+## Documentation
 
-Special thanks and helpful contributors:
+The nf-core/rnafusion pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/rnafusion/usage) and [output](https://nf-co.re/rnafusion/output).
 
-* [Bruce Moran](https://github.com/brucemoran)
-* [Lars Grøntved](https://portal.findresearcher.sdu.dk/en/persons/larsgr)
-* [Monica Nistér](https://ki.se/en/onkpat/research-team-monica-nister)
-* [Phil Ewels](https://github.com/ewels)
-* [Rickard Hammarén](https://github.com/Hammarn)
-* [Szilveszter Juhos](https://github.com/szilvajuhos)
-* [Teresita Díaz de Ståhl](https://ki.se/en/onkpat/teresita-diaz-de-stahls-group)
+<!-- TODO nf-core: Add a brief overview of what the pipeline does and how it works -->
 
-## Tool References
+## Credits
 
-* **STAR-Fusion: Fast and Accurate Fusion Transcript Detection from RNA-Seq**
-Brian Haas, Alexander Dobin, Nicolas Stransky, Bo Li, Xiao Yang, Timothy Tickle, Asma Bankapur, Carrie Ganote, Thomas Doak, Natalie Pochet, Jing Sun, Catherine Wu, Thomas Gingeras, Aviv Regev
-bioRxiv 120295; doi: [https://doi.org/10.1101/120295](https://doi.org/10.1101/120295)
-* D. Nicorici, M. Satalan, H. Edgren, S. Kangaspeska, A. Murumagi, O. Kallioniemi, S. Virtanen, O. Kilkku, **FusionCatcher – a tool for finding somatic fusion genes in paired-end RNA-sequencing data**, bioRxiv, Nov. 2014,
-[DOI:10.1101/011650](http://dx.doi.org/10.1101/011650)
-* Benelli M, Pescucci C, Marseglia G, Severgnini M, Torricelli F, Magi A. **Discovering chimeric transcripts in paired-end RNA-seq data by using EricScript**. Bioinformatics. 2012; 28(24): 3232-3239.
-* **Fusion detection and quantification by pseudoalignment**
-Páll Melsted, Shannon Hateley, Isaac Charles Joseph, Harold Pimentel, Nicolas L Bray, Lior Pachter, bioRxiv 166322; doi: [https://doi.org/10.1101/166322](https://doi.org/10.1101/166322)
-* **SQUID: transcriptomic structural variation detection from RNA-seq** Cong Ma, Mingfu Shao and Carl Kingsford, Genome Biology, 2018, doi: [https://doi.org/10.1186/s13059-018-1421-5](https://doi.org/10.1186/s13059-018-1421-5)
-* **Fusion-Inspector** download: [https://github.com/FusionInspector](https://github.com/FusionInspector)
-* **fusion-report** download: [https://github.com/matq007/fusion-report](https://github.com/matq007/fusion-report); doi: [https://doi.org/10.5281/zenodo.3520171](https://doi.org/10.5281/zenodo.3520171)
-* **FastQC** download: [https://www.bioinformatics.babraham.ac.uk/projects/fastqc/](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
-* **MultiQC** Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. [https://doi.org/10.1093/bioinformatics/btw354](https://doi.org/10.1093/bioinformatics/btw354) Download: [https://multiqc.info/](https://multiqc.info/)
+nf-core/rnafusion was originally written by Martin Proks.
 
 ## Contributions and Support
 
@@ -108,7 +56,8 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 
 ## Citation
 
-If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.151721952](https://zenodo.org/badge/latestdoi/151721952)
+<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi. -->
+<!-- If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
 
 You can cite the `nf-core` publication as follows:
 
@@ -118,7 +67,3 @@ You can cite the `nf-core` publication as follows:
 >
 > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).
 > ReadCube: [Full Access Link](https://rdcu.be/b1GjZ)
-
-[![Barntumörbanken](docs/images/BTB_logo.png)](https://ki.se/forskning/barntumorbanken-0) | [![SciLifeLab](docs/images/SciLifeLab_logo.png)](https://scilifelab.se)
-:-:|:-:
-[![National Genomics Infrastructure](docs/images/NGI_logo.png)](https://ngisweden.scilifelab.se/) | [![University of Southern Denmark](docs/images/SDU_logo.png)](https://www.sdu.dk/da)
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diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
index 1618db14..981e8de6 100755
--- a/bin/scrape_software_versions.py
+++ b/bin/scrape_software_versions.py
@@ -2,35 +2,19 @@
 from __future__ import print_function
 from collections import OrderedDict
 import re
-import os
 
+# TODO nf-core: Add additional regexes for new tools in process get_software_versions
 regexes = {
     "nf-core/rnafusion": ["v_pipeline.txt", r"(\S+)"],
     "Nextflow": ["v_nextflow.txt", r"(\S+)"],
     "FastQC": ["v_fastqc.txt", r"FastQC v(\S+)"],
     "MultiQC": ["v_multiqc.txt", r"multiqc, version (\S+)"],
-    "Arriba": ["v_arriba.txt", r"arriba=(\S+)"],
-    "EricScript": ["v_ericscript.txt", r"ericscript=(\S+)"],
-    "FusionCatcher": ["v_fusioncatcher.txt", r"fusioncatcher=(\S+)"],
-    "Fusion-Inspector": ["v_fusion_inspector.txt", r"fusion-inspector=(\S+)"],
-    "fusion-report": ["v_fusion_report.txt", r"fusion-report=(\S+)"],
-    "Pizzly": ["v_pizzly.txt", r"pizzly=(\S+)"],
-    "STAR-Fusion": ["v_star_fusion.txt", r"star-fusion=(\S+)"],
-    "Squid": ["v_squid.txt", r"squid=(\S+)"]
 }
 results = OrderedDict()
 results["nf-core/rnafusion"] = '<span style="color:#999999;">N/A</span>'
 results["Nextflow"] = '<span style="color:#999999;">N/A</span>'
 results["FastQC"] = '<span style="color:#999999;">N/A</span>'
 results["MultiQC"] = '<span style="color:#999999;">N/A</span>'
-results["Arriba"] = '<span style="color:#999999;\">N/A</span>'
-results["EricScript"] = '<span style="color:#999999;\">N/A</span>'
-results["FusionCatcher"] = '<span style="color:#999999;\">N/A</span>'
-results["Fusion-Inspector"] = '<span style="color:#999999;\">N/A</span>'
-results["fusion-report"] = '<span style="color:#999999;\">N/A</span>'
-results["Pizzly"] = '<span style="color:#999999;\">N/A</span>'
-results["STAR-Fusion"] = '<span style="color:#999999;\">N/A</span>'
-results["Squid"] = '<span style="color:#999999;\">N/A</span>'
 
 # Search each file using its regex
 for k, v in regexes.items():
diff --git a/build-ctat.nf b/build-ctat.nf
deleted file mode 100644
index 6f5dfa78..00000000
--- a/build-ctat.nf
+++ /dev/null
@@ -1,162 +0,0 @@
-#!/usr/bin/env nextflow
-/*
-================================================================================
-                                nf-core/rnafusion
-================================================================================
-nf-core/rnafusion:
- RNA-seq analysis pipeline for detection gene-fusions
---------------------------------------------------------------------------------
- @Homepage
- https://nf-co.re/rnafusion
---------------------------------------------------------------------------------
- @Documentation
- https://nf-co.re/rnafusion/docs
---------------------------------------------------------------------------------
- @Repository
- https://github.com/nf-core/rnafusion
---------------------------------------------------------------------------------
-*/
-
-def helpMessage() {
-    log.info nfcoreHeader()
-    log.info"""
-    Usage:
-
-    The typical command for downloading references is as follows:
-
-    nextflow run nf-core/rnafusion/build-ctat.nf -profile [PROFILE] [OPTIONS] --outdir /path/to/output
-
-    Mandatory arguments:
-      --fasta [file]                Path to fasta reference
-      --gtf [file]                  Path to GTF annotation
-      --genome [str]                Either "GRCh37" or "GRCh38" (default)
-      --outdir [path]               Output directory for downloading
-      -profile [str]                Configuration profile [https://github.com/nf-core/configs]
-    """.stripIndent()
-}
-
-/*
- * SET UP CONFIGURATION VARIABLES
- */
-
-// Show help message
-if (params.help) exit 0, helpMessage()
-
-params.fasta = params.genome ? params.genomes[params.genome].fasta ?: null : null
-params.gtf = params.genome ? params.genomes[params.genome].gtf ?: null : null
-
-ch_fasta = Channel.value(file(params.fasta)).ifEmpty{exit 1, "Fasta file not found: ${params.fasta}"}
-ch_gtf = Channel.value(file(params.gtf)).ifEmpty{exit 1, "GTF annotation file not found: ${params.gtf}"}
-
-if (!params.outdir) exit 1, "Output directory not specified!"
-
-// Header log info
-log.info nfcoreHeader()
-def summary = [:]
-summary['Pipeline Name']  = 'nf-core/rnafusion/build-ctat.nf'
-summary['Pipeline Version'] = workflow.manifest.version
-if(workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container"
-summary['Max Resources']    = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job"
-summary['Output dir']   = params.outdir
-summary['User']         = workflow.userName
-log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n")
-log.info "\033[2m----------------------------------------------------\033[0m"
-
-// Check the hostnames against configured profiles
-checkHostname()
-
-/*
-================================================================================
-                                  DOWNLOAD
-================================================================================
-*/
-
-process star_fusion {
-    label 'process_high'
-    label 'process_long'
-    publishDir "${params.outdir}/star-fusion", mode: 'move'
-
-    input:
-    file(fasta) from ch_fasta
-    file(gtf) from ch_gtf
-
-    output:
-    file 'ctat_genome_lib_build_dir/'
-
-    script:
-    def ctat_url = params.genome == "GRCh37" ? "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/CTAT_HumanFusionLib.v0.1.0/CTAT_HumanFusionLib.v0.1.0.dat.gz" : "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.2.0/fusion_lib.Mar2019.dat.gz"
-    """
-    wget -N ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pfam-A.hmm.gz
-    gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
-
-    wget ${ctat_url} -O CTAT_HumanFusionLib.dat.gz
-
-    # Dfam
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m
-    wget https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p
-
-    export TMPDIR=/tmp
-    prep_genome_lib.pl \\
-        --genome_fa ${fasta} \\
-        --gtf ${gtf} \\
-        --annot_filter_rule /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/lib/STAR-Fusion/ctat-genome-lib-builder/AnnotFilterRuleLib/AnnotFilterRule.pm \\
-        --fusion_annot_lib CTAT_HumanFusionLib.dat.gz \\
-        --pfam_db Pfam-A.hmm \\
-        --dfam_db homo_sapiens_dfam.hmm \\
-        --CPU ${task.cpus}
-    """
-}
-
-/*
- * Completion
- */
-workflow.onComplete {
-    log.info "[nf-core/rnafusion/build-ctat.nf] Pipeline Complete"
-}
-
-def nfcoreHeader() {
-    // Log colors ANSI codes
-    c_black = params.monochrome_logs ? '' : "\033[0;30m";
-    c_blue = params.monochrome_logs ? '' : "\033[0;34m";
-    c_cyan = params.monochrome_logs ? '' : "\033[0;36m";
-    c_dim = params.monochrome_logs ? '' : "\033[2m";
-    c_green = params.monochrome_logs ? '' : "\033[0;32m";
-    c_purple = params.monochrome_logs ? '' : "\033[0;35m";
-    c_reset = params.monochrome_logs ? '' : "\033[0m";
-    c_white = params.monochrome_logs ? '' : "\033[0;37m";
-    c_yellow = params.monochrome_logs ? '' : "\033[0;33m";
-
-    return """    -${c_dim}--------------------------------------------------${c_reset}-
-                                            ${c_green},--.${c_black}/${c_green},-.${c_reset}
-    ${c_blue}        ___     __   __   __   ___     ${c_green}/,-._.--~\'${c_reset}
-    ${c_blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${c_yellow}}  {${c_reset}
-    ${c_blue}  | \\| |       \\__, \\__/ |  \\ |___     ${c_green}\\`-._,-`-,${c_reset}
-                                            ${c_green}`._,._,\'${c_reset}
-    ${c_purple}  nf-core/rnafusion v${workflow.manifest.version}${c_reset}
-    -${c_dim}--------------------------------------------------${c_reset}-
-    """.stripIndent()
-}
-
-def checkHostname() {
-    def c_reset = params.monochrome_logs ? '' : "\033[0m"
-    def c_white = params.monochrome_logs ? '' : "\033[0;37m"
-    def c_red = params.monochrome_logs ? '' : "\033[1;91m"
-    def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m"
-    if (params.hostnames) {
-        def hostname = "hostname".execute().text.trim()
-        params.hostnames.each { prof, hnames ->
-            hnames.each { hname ->
-                if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                    log.error "====================================================\n" +
-                            "  ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" +
-                            "  but your machine hostname is ${c_white}'$hostname'${c_reset}\n" +
-                            "  ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" +
-                            "============================================================"
-                }
-            }
-        }
-    }
-}
diff --git a/conf/base.config b/conf/base.config
index 0081fde2..e2a4c1ac 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -9,26 +9,23 @@
  * run on the logged in environment.
  */
 
-params {
-    versions {
-    arriba = '1.2.0'
-    ericscript = '0.5.5'
-    fusioncatcher = '1.20'
-    pizzly = '0.37.3'
-    squid = '1.5-star2.7.1a'
-    star_fusion = '1.8.1'
-  }
-}
-
 process {
+
+  // TODO nf-core: Check the defaults for all processes
   cpus = { check_max( 1 * task.attempt, 'cpus' ) }
   memory = { check_max( 7.GB * task.attempt, 'memory' ) }
-  time = { check_max( 6.h * task.attempt, 'time' ) }
+  time = { check_max( 4.h * task.attempt, 'time' ) }
 
   errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' }
   maxRetries = 1
   maxErrors = '-1'
 
+  // Process-specific resource requirements
+  // NOTE - Only one of the labels below are used in the fastqc process in the main script.
+  //        If possible, it would be nice to keep the same label naming convention when
+  //        adding in your processes.
+  // TODO nf-core: Customise requirements for specific processes.
+  // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors
   withLabel:process_low {
     cpus = { check_max( 2 * task.attempt, 'cpus' ) }
     memory = { check_max( 14.GB * task.attempt, 'memory' ) }
@@ -37,37 +34,18 @@ process {
   withLabel:process_medium {
     cpus = { check_max( 6 * task.attempt, 'cpus' ) }
     memory = { check_max( 42.GB * task.attempt, 'memory' ) }
-    time = { check_max( 12.h * task.attempt, 'time' ) }
+    time = { check_max( 8.h * task.attempt, 'time' ) }
   }
   withLabel:process_high {
     cpus = { check_max( 12 * task.attempt, 'cpus' ) }
     memory = { check_max( 84.GB * task.attempt, 'memory' ) }
-    time = { check_max( 24.h * task.attempt, 'time' ) }
+    time = { check_max( 10.h * task.attempt, 'time' ) }
   }
   withLabel:process_long {
-    time = { check_max( 48.h * task.attempt, 'time' ) }
+    time = { check_max( 20.h * task.attempt, 'time' ) }
   }
   withName:get_software_versions {
     cache = false
   }
-
-  // Fusion tools
-  withName:"arriba|arriba_visualization" {
-    container = "nfcore/rnafusion:arriba_${params.versions.arriba}"
-  }
-  withName:ericscript {
-    container = "nfcore/rnafusion:ericscript_${params.versions.ericscript}"
-  }
-  withName:fusioncatcher {
-    container = "nfcore/rnafusion:fusioncatcher_${params.versions.fusioncatcher}"
-  }
-  withName:pizzly {
-    container = "nfcore/rnafusion:pizzly_${params.versions.pizzly}"
-  }
-  withName:squid {
-    container = "nfcore/rnafusion:squid_${params.versions.squid}"
-  }
-  withName:"star_fusion|fusion_inspector" {
-    container = "nfcore/rnafusion:star-fusion_${params.versions.star_fusion}"
-  }
-}
\ No newline at end of file
+  
+}
diff --git a/conf/genomes.config b/conf/genomes.config
deleted file mode 100644
index 934041b2..00000000
--- a/conf/genomes.config
+++ /dev/null
@@ -1,33 +0,0 @@
-/*
- * -------------------------------------------------
- *  Nextflow config file for reference genome
- * -------------------------------------------------
- * Defines reference genomes, without using iGenome paths
- * Can be used by any config that customizes the base
- * path using $params.genomes_base / --genomes_base
- */
-
-params {
-    genomes {
-        'GRCh38' {
-            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh38_r${params.reference_release}.all.fa"
-            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh38_r${params.reference_release}.gtf"
-            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh38_r${params.reference_release}.cdna.all.fa.gz"
-            databases           = "${params.genomes_base}/databases"
-            arriba_ref          = "${params.genomes_base}/arriba"
-            ericscript_ref      = "${params.genomes_base}/ericscript/ericscript_db_homosapiens_ensembl84"
-            fusioncatcher_ref   = "${params.genomes_base}/fusioncatcher/human_v98"
-            star_fusion_ref     = "${params.genomes_base}/star-fusion/ctat_genome_lib_build_dir"
-        }
-        'GRCh37' {
-            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.all.fa"
-            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.gtf"
-            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.cdna.all.fa.gz"
-            databases           = "${params.genomes_base}/databases"
-            arriba_ref          = "${params.genomes_base}/arriba"
-            ericscript_ref      = "${params.genomes_base}/ericscript/ericscript_db_homosapiens_ensembl73"
-            fusioncatcher_ref   = "${params.genomes_base}/fusioncatcher/README.txt"
-            star_fusion_ref     = "${params.genomes_base}/star-fusion/ctat_genome_lib_build_dir"
-        }
-    }
-}
diff --git a/conf/igenomes.config b/conf/igenomes.config
new file mode 100644
index 00000000..caeafceb
--- /dev/null
+++ b/conf/igenomes.config
@@ -0,0 +1,421 @@
+/*
+ * -------------------------------------------------
+ *  Nextflow config file for iGenomes paths
+ * -------------------------------------------------
+ * Defines reference genomes, using iGenome paths
+ * Can be used by any config that customises the base
+ * path using $params.igenomes_base / --igenomes_base
+ */
+
+params {
+  // illumina iGenomes reference file paths
+  genomes {
+    'GRCh37' {
+      fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
+      mito_name   = "MT"
+      macs_gsize  = "2.7e9"
+      blacklist   = "${baseDir}/assets/blacklists/GRCh37-blacklist.bed"
+    }
+    'GRCh38' {
+      fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
+      mito_name   = "chrM"
+      macs_gsize  = "2.7e9"
+      blacklist   = "${baseDir}/assets/blacklists/hg38-blacklist.bed"
+    }
+    'GRCm38' {
+      fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
+      mito_name   = "MT"
+      macs_gsize  = "1.87e9"
+      blacklist   = "${baseDir}/assets/blacklists/GRCm38-blacklist.bed"
+    }
+    'TAIR10' {
+      fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
+      mito_name   = "Mt"
+    }
+    'EB2' {
+      fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
+    }
+    'UMD3.1' {
+      fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
+      mito_name   = "MT"
+    }
+    'WBcel235' {
+      fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
+      mito_name   = "MtDNA"
+      macs_gsize  = "9e7"
+    }
+    'CanFam3.1' {
+      fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
+      mito_name   = "MT"
+    }
+    'GRCz10' {
+      fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
+      mito_name   = "MT"
+    }
+    'BDGP6' {
+      fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
+      mito_name   = "M"
+      macs_gsize  = "1.2e8"
+    }
+    'EquCab2' {
+      fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
+      mito_name   = "MT"
+    }
+    'EB1' {
+      fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
+    }
+    'Galgal4' {
+      fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
+      mito_name   = "MT"
+    }
+    'Gm01' {
+      fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
+    }
+    'Mmul_1' {
+      fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
+      mito_name   = "MT"
+    }
+    'IRGSP-1.0' {
+      fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
+      mito_name   = "Mt"
+    }
+    'CHIMP2.1.4' {
+      fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
+      mito_name   = "MT"
+    }
+    'Rnor_6.0' {
+      fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
+      mito_name   = "MT"
+    }
+    'R64-1-1' {
+      fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
+      mito_name   = "MT"
+      macs_gsize  = "1.2e7"
+    }
+    'EF2' {
+      fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
+      mito_name   = "MT"
+      macs_gsize  = "1.21e7"
+    }
+    'Sbi1' {
+      fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
+    }
+    'Sscrofa10.2' {
+      fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
+      mito_name   = "MT"
+    }
+    'AGPv3' {
+      fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
+      mito_name   = "Mt"
+    }
+    'hg38' {
+      fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
+      mito_name   = "chrM"
+      macs_gsize  = "2.7e9"
+      blacklist   = "${baseDir}/assets/blacklists/hg38-blacklist.bed"
+    }
+    'hg19' {
+      fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
+      mito_name   = "chrM"
+      macs_gsize  = "2.7e9"
+      blacklist   = "${baseDir}/assets/blacklists/hg19-blacklist.bed"
+    }
+    'mm10' {
+      fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
+      mito_name   = "chrM"
+      macs_gsize  = "1.87e9"
+      blacklist   = "${baseDir}/assets/blacklists/mm10-blacklist.bed"
+    }
+    'bosTau8' {
+      fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
+      mito_name   = "chrM"
+    }
+    'ce10' {
+      fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
+      mito_name   = "chrM"
+      macs_gsize  = "9e7"
+    }
+    'canFam3' {
+      fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
+      mito_name   = "chrM"
+    }
+    'danRer10' {
+      fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
+      mito_name   = "chrM"
+      macs_gsize  = "1.37e9"
+    }
+    'dm6' {
+      fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
+      mito_name   = "chrM"
+      macs_gsize  = "1.2e8"
+    }
+    'equCab2' {
+      fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
+      mito_name   = "chrM"
+    }
+    'galGal4' {
+      fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
+      mito_name   = "chrM"
+    }
+    'panTro4' {
+      fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
+      mito_name   = "chrM"
+    }
+    'rn6' {
+      fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
+      mito_name   = "chrM"
+    }
+    'sacCer3' {
+      fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
+      readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
+      mito_name   = "chrM"
+      macs_gsize  = "1.2e7"
+    }
+    'susScr3' {
+      fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
+      bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
+      bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
+      star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
+      bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
+      gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
+      bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
+      readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
+      mito_name   = "chrM"
+    }
+  }
+}
diff --git a/conf/test.config b/conf/test.config
index 08c1db37..c84e3197 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -15,6 +15,12 @@ params {
   max_memory = 6.GB
   max_time = 48.h
 
-  // Impossible to CI test rnafusion due to huge indexes requirements
-  help = true
+  // Input data
+  // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
+  // TODO nf-core: Give any required params for the test so that command line flags are not needed
+  single_end = false
+  input_paths = [
+    ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']],
+    ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']]
+  ]
 }
diff --git a/containers/arriba/Dockerfile b/containers/arriba/Dockerfile
deleted file mode 100644
index f6a94950..00000000
--- a/containers/arriba/Dockerfile
+++ /dev/null
@@ -1,14 +0,0 @@
-FROM nfcore/base:1.9
-
-LABEL authors="Martin Proks" \
-    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
-
-# Install the conda environment
-COPY environment.yml /
-RUN conda env create -f /environment.yml && conda clean -a
-
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-arriba_1.2.0/bin:$PATH
-
-# Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-arriba_1.2.0 > nf-core-rnafusion-arriba_1.2.0.yml
\ No newline at end of file
diff --git a/containers/arriba/environment.yml b/containers/arriba/environment.yml
deleted file mode 100644
index 393b2e11..00000000
--- a/containers/arriba/environment.yml
+++ /dev/null
@@ -1,14 +0,0 @@
-name: nf-core-rnafusion-arriba_1.2.0
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::arriba=1.2.0
-  - bioconda::bioconductor-genomicalignments
-  - bioconda::bioconductor-genomicranges
-  - bioconda::samtools=1.9
-  - bioconda::star=2.7.1a
-  - conda-forge::openssl=1.0
-  - conda-forge::r-circlize
-  - conda-forge::readline=6.2
\ No newline at end of file
diff --git a/containers/ericscript/Dockerfile b/containers/ericscript/Dockerfile
deleted file mode 100644
index d418d1c7..00000000
--- a/containers/ericscript/Dockerfile
+++ /dev/null
@@ -1,17 +0,0 @@
-FROM nfcore/base:1.9
-
-LABEL authors="Martin Proks" \
-    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
-
-# Install the conda environment
-COPY environment.yml /
-RUN conda env create -f /environment.yml && conda clean -a
-
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-ericscript_0.5.5/bin:$PATH
-
-# Ignore database check (https://github.com/nf-core/rnafusion/issues/119)
-RUN echo 1 > /opt/conda/envs/nf-core-rnafusion-ericscript_0.5.5/share/ericscript-0.5.5-4/lib/data/_resources/.flag.dbexists
-
-# Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-ericscript_0.5.5 > nf-core-rnafusion-ericscript_0.5.5.yml
\ No newline at end of file
diff --git a/containers/ericscript/environment.yml b/containers/ericscript/environment.yml
deleted file mode 100644
index 3edc48ce..00000000
--- a/containers/ericscript/environment.yml
+++ /dev/null
@@ -1,8 +0,0 @@
-name: nf-core-rnafusion-ericscript_0.5.5
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::ericscript=0.5.5
-  - conda-forge::ncurses=6.1
\ No newline at end of file
diff --git a/containers/fusioncatcher/Dockerfile b/containers/fusioncatcher/Dockerfile
deleted file mode 100644
index 203bb32a..00000000
--- a/containers/fusioncatcher/Dockerfile
+++ /dev/null
@@ -1,14 +0,0 @@
-FROM nfcore/base:1.7
-
-LABEL authors="Martin Proks" \
-    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
-
-# Install the conda environment
-COPY environment.yml /
-RUN conda env create -f /environment.yml && conda clean -a
-
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-fusioncatcher_1.20/bin:$PATH
-
-# Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-fusioncatcher_1.20 > nf-core-rnafusion-fusioncatcher_1.20.yml
\ No newline at end of file
diff --git a/containers/fusioncatcher/environment.yml b/containers/fusioncatcher/environment.yml
deleted file mode 100644
index 1d6a105d..00000000
--- a/containers/fusioncatcher/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-name: nf-core-rnafusion-fusioncatcher_1.20
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::fusioncatcher=1.20
diff --git a/containers/pizzly/Dockerfile b/containers/pizzly/Dockerfile
deleted file mode 100644
index 2817069f..00000000
--- a/containers/pizzly/Dockerfile
+++ /dev/null
@@ -1,14 +0,0 @@
-FROM nfcore/base:1.9
-
-LABEL authors="Martin Proks" \
-    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
-
-# Install the conda environment
-COPY environment.yml /
-RUN conda env create -f /environment.yml && conda clean -a
-
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-pizzly_0.37.3/bin:$PATH
-
-# Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-pizzly_0.37.3 > nf-core-rnafusion-pizzly_0.37.3.yml
\ No newline at end of file
diff --git a/containers/pizzly/environment.yml b/containers/pizzly/environment.yml
deleted file mode 100644
index 79974871..00000000
--- a/containers/pizzly/environment.yml
+++ /dev/null
@@ -1,9 +0,0 @@
-name: nf-core-rnafusion-pizzly_0.37.3
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::kallisto=0.44.0
-  - bioconda::pizzly=0.37.3
-  - conda-forge::pigz=2.3.4
diff --git a/containers/squid/Dockerfile b/containers/squid/Dockerfile
deleted file mode 100644
index 5dbcaf4c..00000000
--- a/containers/squid/Dockerfile
+++ /dev/null
@@ -1,19 +0,0 @@
-FROM nfcore/base:1.9
-
-LABEL authors="Martin Proks" \
-    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
-
-# Install the conda environment
-COPY environment.yml /
-RUN conda env create -f /environment.yml && conda clean -a
-
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin:$PATH
-
-RUN cd /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin \
-    && wget https://raw.githubusercontent.com/Kingsford-Group/squid/f45c9025d41cffd982ecbbdd52844e5a4f074de9/utils/AnnotateSQUIDOutput.py \
-    && chmod +x /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin/AnnotateSQUIDOutput.py \
-    && ln -s /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin/python3 /bin/python
-
-# Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-squid_1.5-star2.7.1a > nf-core-rnafusion-squid_1.5-star2.7.1a.yml
\ No newline at end of file
diff --git a/containers/squid/environment.yml b/containers/squid/environment.yml
deleted file mode 100644
index 7385b163..00000000
--- a/containers/squid/environment.yml
+++ /dev/null
@@ -1,11 +0,0 @@
-name: nf-core-rnafusion-squid_1.5-star2.7.1a
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::samtools=1.9
-  - bioconda::squid=1.5
-  - bioconda::star=2.7.1a
-  - conda-forge::numpy=1.18.1
-  - conda-forge::python=3.7.6
diff --git a/containers/star-fusion/Dockerfile b/containers/star-fusion/Dockerfile
deleted file mode 100644
index 79c6fdbc..00000000
--- a/containers/star-fusion/Dockerfile
+++ /dev/null
@@ -1,20 +0,0 @@
-FROM nfcore/base:1.9
-
-LABEL authors="Martin Proks" \
-    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
-
-# Install the conda environment
-COPY environment.yml /
-RUN conda env create -f /environment.yml && conda clean -a
-
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/bin:$PATH
-
-# FusionInspector
-ENV PATH /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/lib/STAR-Fusion/FusionInspector:$PATH
-
-# ctat-genome-lib-builder
-ENV PATH /opt/conda/envs/nf-core-rnafusion-star-fusion_1.8.1/lib/STAR-Fusion/ctat-genome-lib-builder:$PATH
-
-# Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-star-fusion_1.8.1 > nf-core-rnafusion-star-fusion_1.8.1.yml
\ No newline at end of file
diff --git a/containers/star-fusion/environment.yml b/containers/star-fusion/environment.yml
deleted file mode 100644
index 4ca4be91..00000000
--- a/containers/star-fusion/environment.yml
+++ /dev/null
@@ -1,12 +0,0 @@
-name: nf-core-rnafusion-star-fusion_1.8.1
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::dfam=2.0
-  - bioconda::hmmer=3.2.1
-  - bioconda::star-fusion=1.8.1
-  - bioconda::trinity=2.6.6
-  - bioconda::samtools=1.9
-  - conda-forge::perl-carp-assert
\ No newline at end of file
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diff --git a/docs/output.md b/docs/output.md
index e5ca8955..e28adb3e 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -1,78 +1,25 @@
-# nf-core/rnafusion: Output <!-- omit in toc -->
+# nf-core/rnafusion: Output
 
-This document describes the output produced by the pipeline.
-Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline.
+## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/rnafusion/output](https://nf-co.re/rnafusion/output)
 
-The directories listed below will be created in the results directory after the pipeline has finished.
-All paths are relative to the top-level results directory.
+> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._
 
-## Pipeline overview <!-- omit in toc -->
+## Introduction
 
-The pipeline is built using [Nextflow](https://www.nextflow.io/)
-and processes data using the following steps:
-
-- [Arriba](#arriba)
-- [EricScript](#ericscript)
-- [FastQC](#fastqc)
-- [Fusioncatcher](#fusioncatcher)
-- [Fusion Inspector](#fusion-inspector)
-- [fusion-report](#fusion-report)
-  - [Tool detection](#tool-detection)
-  - [Found in database](#found-in-database)
-  - [Tool detection distribution](#tool-detection-distribution)
-- [MultiQC](#multiqc)
-- [Pipeline information](#pipeline-information)
-- [Pizzly](#pizzly)
-- [Squid](#squid)
-- [Star-Fusion](#star-fusion)
-
-## Arriba
-
-**Output directory: `results/tools/Arriba`**
-
-- `fusions.tsv`
-  - contains fusions which pass all of Arriba's filters. It should be highly enriched for true predictions. The predictions are listed from highest to lowest confidence.
-- `fusions.discarded.tsv`
-  - contains all events that Arriba classified as an artifact or that are also observed in healthy tissue. This file may be useful, if one suspects that an event should be present, but was erroneously discarded by Arriba.
-- `<sample>.pdf`
-  - contains fusion visualization when opted for `--arriba_vis`
+This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline.
 
-## EricScript
+The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory.
 
-**Output directory: `results/tools/Ericscript/tmp`**
+<!-- TODO nf-core: Write this documentation describing your workflow's output -->
 
-- `fusions.results.filtered.tsv`
-  - contains all the predicted gene fusions
+## Pipeline overview
 
-|      Column      |                                        Description                                        |
-| ---------------- | ----------------------------------------------------------------------------------------- |
-| GeneName1        | official gene name of 5' gene.                                                            |
-| GeneName2        | official gene name of 3' gene.                                                            |
-| chr1             | chromosome of 5' gene.                                                                    |
-| Breakpoint1      | predicted breakpoint on 5' gene.                                                          |
-| strand1          | strand (-/+) of 5' gene.                                                                  |
-| chr2             | chromosome of 3' gene.                                                                    |
-| Breakpoint2      | predicted breakpoint on 3' gene.                                                          |
-| strand2          | strand (-/+) of 3' gene.                                                                  |
-| EnsemblGene1     | Ensembl gene ID of 5' gene.                                                               |
-| EnsemblGene2     | Ensembl gene ID of 3' gene.                                                               |
-| crossingreads    | number of paired end discordant reads.                                                    |
-| spanningreads    | number of paired end reads spanning the junction.                                         |
-| mean.insertsize  | mean of insert sizes of crossing + spanning reads.                                        |
-| homology         | if filled, all the homologies between the fusion junction and Ensembl genes.              |
-| fusiontype       | intra-chromosomal, inter-chromosomal, read-through or CIS.                                |
-| InfoGene1        | gene information about 5' gene.                                                           |
-| InfoGene2        | gene information about 3' gene.                                                           |
-| JunctionSequence | predicted junction fusion sequence.                                                       |
-| GeneExpr1        | Read count based estimation of the expression level of 5' gene.                           |
-| GeneExpr2        | Read count based estimation of the expression level of 3' gene.                           |
-| GeneExpr_fused   | Read count based estimation of the expression level of the predicted chimeric transcript. |
-| ES               | Edge score.                                                                               |
-| GJS              | Genuine Junction score.                                                                   |
-| US               | Uniformity score.                                                                         |
-| EricScore        | EricScore score (adaboost classifier).                                                    |
+The pipeline is built using [Nextflow](https://www.nextflow.io/)
+and processes data using the following steps:
 
-For more info check the [documentation](https://sites.google.com/site/bioericscript/getting-started).
+* [FastQC](#fastqc) - Read quality control
+* [MultiQC](#multiqc) - Aggregate report describing results from the whole pipeline
+* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ## FastQC
 
@@ -82,107 +29,12 @@ For further reading and documentation see the [FastQC help pages](http://www.bio
 
 **Output files:**
 
-> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads.
-> They may contain adapter sequence and potentially regions with low quality.
-
-- `fastqc/`
-  - `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files.
-- `fastqc/zips/`
-  - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
-
-## Fusioncatcher
-
-**Output directory: `results/tools/Fusioncatcher`**
-
-- `final-list_candidate-fusion-genes.txt`
-  - contains all the predicted gene fusions
-
-| Column | Description |
-| ------ | ----------- |
-| **Gene\_1\_symbol(5end\_fusion\_partner)** | Gene symbol of the 5' end fusion partner |
-| **Gene\_2\_symbol\_2(3end\_fusion\_partner)** | Gene symbol of the 3' end fusion partner |
-| **Gene\_1\_id(5end\_fusion\_partner)** | Ensembl gene id of the 5' end fusion partner |
-| **Gene\_2\_id(3end\_fusion\_partner)** | Ensembl gene id of the 3' end fusion partner |
-| **Exon\_1\_id(5end\_fusion\_partner)** | Ensembl exon id of the 5' end fusion exon-exon junction |
-| **Exon\_2\_id(3end\_fusion\_partner)** | Ensembl exon id of the 3' end fusion exon-exon junction |
-| **Fusion\_point\_for\_gene\_1(5end\_fusion\_partner)** | Chromosomal position of the 5' end of fusion junction (chromosome:position:strand); 1-based coordinate |
-| **Fusion\_point\_for\_gene\_2(3end\_fusion\_partner)** | Chromosomal position of the 3' end of fusion junction (chromosome:position:strand); 1-based coordinate |
-| **Spanning\_pairs** | Count of pairs of reads supporting the fusion (**including** also the multimapping reads) |
-| **Spanning\_unique\_reads** | Count of unique reads (i.e. unique mapping positions) mapping on the fusion junction. Shortly, here are counted all the reads which map on fusion junction minus the PCR duplicated reads. |
-| **Longest\_anchor\_found** | Longest anchor (hangover) found among the unique reads mapping on the fusion junction |
-| **Fusion\_finding\_method** | Aligning method used for mapping the reads and finding the fusion genes. Here are the methods used: |
-| | (i) **BOWTIE** = only Bowtie aligner is used for mapping the reads on the genome and exon-exon fusion junctions |
-| | (ii) **BOWTIE+BLAT** = Bowtie aligner is used for mapping reads on the genome and BLAT is used for mapping reads for finding the fusion junction |
-| | (iii) **BOWTIE+STAR** = Bowtie aligner is used for mapping reads on the genome and STAR is used for mapping reads for finding the fusion junction |
-| | (iv) **BOWTIE+BOWTIE2** = Bowtie aligner is used for mapping reads on the genome and Bowtie2 is used for mapping reads for finding the fusion junction |
-| **Fusion\_sequence** | The inferred fusion junction (the asterisk sign marks the junction point) |
-| **Fusion\_description** | Type of the fusion gene (see the Table 2) |
-| **Counts\_of\_common\_mapping\_reads** | Count of reads mapping simultaneously on both genes which form the fusion gene. This is an indication how similar are the DNA/RNA sequences of the genes forming the fusion gene (i.e. what is their homology because highly homologous genes tend to appear show as candidate fusion genes). In case of completely different sequences of the genes involved in forming a fusion gene then here it is expected to have the value zero. |
-| **Predicted\_effect** | Predicted effect of the candidate fusion gene using the annotation from Ensembl database. This is shown in format **effect\_gene\_1**/**effect\_gene\_2**, where the possible values for effect\_gene\_1 or effect\_gene\_2 are: **intergenic**, **intronic**, **exonic(no-known-CDS)**, **UTR**, **CDS(not-reliable-start-or-end)**, **CDS(truncated)**, or **CDS(complete)**. In case that the fusion junction for both genes is within their CDS (coding sequence) then only the values **in-frame** or **out-of-frame** will be shown. |
-| **Predicted\_fused\_transcripts** | All possible known fused transcripts in format ENSEMBL-TRANSCRIPT-1:POSITION-1/ENSEMBLE-TRANSCRIPT-B:POSITION-2, where are fused the sequence 1:POSITION-1 of transcript ENSEMBL-TRANSCRIPT-1 with sequence POSITION-2:END of transcript ENSEMBL-TRANSCRIPT-2 |
-| **Predicted\_fused\_proteins** | Predicted amino acid sequences of all possible fused proteins (separated by ";").  |
-
-For more info check the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md#62---output-data-output-data).
-
-## Fusion Inspector
-
-**Output directory: `results/tools/FusionInspector`**
-
-- `finspector.fa`
-  - the candidate fusion-gene contigs (if you copy things elsewhere, make sure to also copy the index file: `finspector.fa.fai`)
-- `finspector.bed`
-  - the reference gene structure annotations for fusion partners
-- `finspector.junction_reads.bam`
-  - alignments of the breakpoint-junction supporting reads.
-- `finspector.spanning_reads.bam`
-  - alignments of the breakpoint-spanning paired-end reads.
-
-To visualize fusion genes in [IGV tool](https://software.broadinstitute.org/software/igv/igvtools) first create a genome `Menu->Genomes->Create .genome File`, choose name and description, then choose the following files:
-
-- `finspector.fa`
-  - make sure the index file finspector.fa.fai is in the same folder
-- `finspector.gtf`
-  - use this for 'Genes'
-- `cytoBand.txt`
-  - use this for 'optional Cytoband'
-
-Add the bam files by choosing `File->Load from File` and make sure to select your generated mini genome in the upper-left corner.
-For more info and help check [wiki page](https://github.com/FusionInspector/FusionInspector/wiki).
-
-## fusion-report
-
-**Output directory: `results/Report-<READS_BASE_NAME>`**
-
-- `fusions.json`
-  - contains all main information about found fusions (fusion name, score, explanation of the score calculation, cherry picked output from fusion tools)
-- `index.html`
-  - main dashboard containing the list of all detected fusions
-- `*.html`
-  - each fusion gets a custom page with fetched data from the local database
-- `fusions_list_filtered.txt`
-  - filtered list of found fusions (uses tool cutoff as filter, by default: 2, can be adjusted by adding `-t <num>` when running the tool)
-- `fusions_list.txt`
-  - unfiltered list of found fusions
-- `<READS_BASE_NAME>_fusion-report.tar.gz`
-  - tarball of the above files to allow easier import to local machine(s) for visualisation through browser
+* `fastqc/`
+  * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files.
+* `fastqc/zips/`
+  * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
-### Tool detection
-
-Graphs displaying ratio of fusion genes caught by different tools. The last part *all tools* is an intersection of all tools.
-
-![Tool detection](images/summary_graph_1.png)
-
-### Found in database
-
-Displays how many fusions were found in a downloaded databases of the summary report.
-
-![Known/unknown fusions](images/summary_graph_2.png)
-
-### Tool detection distribution
-
-For each fusion a sum of detected tools is calculated. This counts are then visualized in the graph below.
-
-![Known/unknown fusions](images/summary_graph_3.png)
+> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
 
 ## MultiQC
 
@@ -194,87 +46,18 @@ For more information about how to use MultiQC reports, see [https://multiqc.info
 
 **Output files:**
 
-- `multiqc/`  
-  - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
-  - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
-  - `multiqc_plots/`: directory containing static images from the report in various formats.
+* `multiqc/`
+  * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
+  * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
+  * `multiqc_plots/`: directory containing static images from the report in various formats.
 
 ## Pipeline information
 
-[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline.
-This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
+[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
 
 **Output files:**
 
-- `pipeline_info/`
-  - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
-  - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`.
-  - Documentation for interpretation of results in HTML format: `results_description.html`.
-
-## Pizzly
-
-**Output directory: `results/tools/Pizzly`**
-
-- `pizzly_fusions.json`
-  - contains all the predicted gene fusions
-
-|  Column | Description |
-| ------- | ----------- |
-| geneA | `id`: reference id and `name`: gene name |
-| geneB | Describes reference id and gene name |
-| paircount | Number of paired count |
-| splitcount | Number of split count |
-| transcripts | List of all transcripts `fasta_record`, `transcriptA`, `transcriptB`, `support`, `reads` |
-| readpairs | List of read pairs containing (`type`, `read1`, `read2`) |
-
-For more info check the [documentation](https://github.com/pmelsted/pizzly#output).
-
-## Squid
-
-**Output directory: `results/tools/Squid`**
-
-- `fusions_annotated.txt`
-  - contains all the predicted gene fusions
-
-|  Column | Description |
-| ------- | ----------- |
-| chr1 | chromosome name of the first breakpoint.
-| start1 | starting position of the segment of the first breakpoint, or the predicted breakpoint position if strand1 is "-" |
-| end1 | ending position of the segment of the first breakpoint, or the predicted breakpoint position if strand1 is "+" |
-| chr2 | chromosome name of the second breakpoint |
-| start2 | starting position of the segment of the second breakpoint, or the predicted breakpoint position if strand2 is "-" |
-| end2 | ending position of the segment of the second breakpoint, or the predicted breakpoint position if strand2 is "+" |
-| name | TSV is not named yet, this column shows with dot.
-| score | number of reads supporting this TSV (without weighted by Discordant edge ratio multiplier) |
-| strand1 | strand of the first segment in TSV.
-| strand2 | strand of the second segment in TSV.
-| num_concordantfrag_bp1 | number of concordant paired-end reads covering the first breakpoint. For a concordant paired-end read, it includes two ends and a inserted region in between, if any of the 3 regions covers the breakpoint, the read is counted in this number |
-| num_concordantfrag_bp2 | number of concordant paired-end reads covering the second breakpoint. The count is defined in the same way as num_concordantfrag_bp1 |
-
-For more info check the [documentation](https://github.com/Kingsford-Group/squid#output-specification).
-
-## Star-Fusion
-
-**Output directory: `results/tools/StarFusion`**
-
-- `star-fusion.fusion_predictions.tsv`
-  - contains all the predicted gene fusions
-
-|  Column | Description |
-| ------- | ----------- |
-| JunctionReadCount | Indicates the number of RNA-Seq fragments containing a read that aligns as a split read at the site of the putative fusion junction. |
-| SpanningFragCount | Indicates the number of RNA-Seq fragments that encompass the fusion junction such that one read of the pair aligns to a different gene than the other paired-end read of that fragment. |
-| SpliceType | Indicates whether the proposed breakpoint occurs at reference exon junctions as provided by the reference transcript structure annotations (ex. gencode).
-| LeftGene
-| LeftBreakpoint
-| RightGene
-| RightBreakpoint
-| LargeAnchorSupport | column indicates whether there are split reads that provide 'long' (set to length of 25 bases) alignments on both sides of the putative breakpoint. |
-| FFPM | fusion fragments per million total reads; **Default:** *0.1 (meaning at least 1 fusion-supporting rna-seq fragment per 10M total reads)*; **TL;DR:** can be adjusted by changing `--min_FFPM`
-| LeftBreakDinuc |  |
-| LeftBreakEntropy | Represents Shannon entropy |
-| RightBreakDinuc |
-| RightBreakEntropy | Represents Shannon entropy |
-| annots | Annotation generated by [FusionAnnotar](https://github.com/FusionAnnotator/FusionAnnotator/wiki) |
-
-For more info check the [documentation](https://github.com/STAR-Fusion/STAR-Fusion/wiki#Outputs).
+* `pipeline_info/`
+  * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
+  * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`.
+  * Documentation for interpretation of results in HTML format: `results_description.html`.
diff --git a/docs/usage.md b/docs/usage.md
index 91f6d4b9..dd7292d5 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -1,174 +1,23 @@
-# nf-core/rnafusion: Usage <!-- omit in toc -->
-
-- [Download references](#download-references)
-  - [Download all references](#download-all-references)
-  - [Download specific references](#download-specific-references)
-  - [Download and build CTAT](#download-and-build-ctat)
-  - [Download GRCh37 references](#download-grch37-references)
-  - [Tool reference requirements](#tool-reference-requirements)
-- [Running the pipeline](#running-the-pipeline)
-  - [Updating the pipeline](#updating-the-pipeline)
-  - [Reproducibility](#reproducibility)
-- [Core Nextflow arguments](#core-nextflow-arguments)
-  - [-profile](#-profile)
-  - [-resume](#-resume)
-  - [-c](#-c)
-    - [Custom resource requests](#custom-resource-requests)
-  - [Running in the background](#running-in-the-background)
-    - [Nextflow memory requirements](#nextflow-memory-requirements)
-- [Pipeline specific arguments](#pipeline-specific-arguments)
-  - [--input](#--input)
-  - [--single_end](#--single_end)
-  - [Tool flags](#tool-flags)
-  - [--arriba](#--arriba)
-  - [--ericscript](#--ericscript)
-  - [--fusioncatcher](#--fusioncatcher)
-  - [--fusion_report](#--fusion_report)
-  - [--pizzly](#--pizzly)
-  - [--squid](#--squid)
-  - [--star_fusion](#--star_fusion)
-- [Visualization flags](#visualization-flags)
-  - [--arriba_vis](#--arriba_vis)
-  - [--fusion_inspector](#--fusion_inspector)
-- [Reference genomes](#reference-genomes)
-  - [--arriba_ref](#--arriba_ref)
-  - [--databases](#--databases)
-  - [--ericscript_ref](#--ericscript_ref)
-  - [--fasta](#--fasta)
-  - [--fusioncatcher_ref](#--fusioncatcher_ref)
-  - [--genome](#--genome)
-  - [--gtf](#--gtf)
-  - [--reference_release](#--reference_release)
-  - [--star_index](#--star_index)
-  - [--star_fusion_ref](#--star_fusion_ref)
-  - [--transcript](#--transcript)
-- [Other command line parameters](#other-command-line-parameters)
-  - [--debug](#--debug)
-  - [--read_length](#--read_length)
-  - [--outdir](#--outdir)
-  - [--email](#--email)
-  - [--email_on_fail](#--email_on_fail)
-  - [--max_multiqc_email_size](#--max_multiqc_email_size)
-  - [-name](#-name)
-  - [--custom_config_version](#--custom_config_version)
-  - [--custom_config_base](#--custom_config_base)
-- [Job resources](#job-resources)
-  - [Automatic resubmission](#automatic-resubmission)
-
-## Download references
-
-The rnafusion pipeline needs references for the fusion detection tools, so downloading these is a prerequisite.
-
-Downloading references manually is a tedious long process.
-To make the pipeline easier to work with, we provide a script to download all necessary references for fusion detection tools.
-
-> **TL;DR:** Make sure to download the correct references for your need!
-
-### Download all references
+# nf-core/rnafusion: Usage
 
-```bash
-# Replace <COSMIC_USER> and <COSMIC_PASSWD> with yout credentials from COSMIC
-nextflow run nf-core/rnafusion/download-references.nf \
-  --download_all \
-  --outdir <PATH> \
-  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
-```
-
-### Download specific references
-
-```bash
-# Example of downloading base references
-
-nextflow run nf-core/rnafusion/download-references.nf \
---base \
---outdir <PATH>
-
-# Example of downloading references for specific tool
-
-nextflow run nf-core/rnafusion/download-references.nf \
---arriba \
---outdir <PATH>
-
-nextflow run nf-core/rnafusion/download-references.nf \
---star_fusion \
---outdir <PATH>
-
-nextflow run nf-core/rnafusion/download-references.nf \
---fusioncatcher \
---outdir <PATH>
+## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/rnafusion/usage](https://nf-co.re/rnafusion/usage)
 
-nextflow run nf-core/rnafusion/download-references.nf \
---ericscript \
---outdir <PATH>
-
-nextflow run nf-core/rnafusion/download-references.nf \
---fusion_report \
---outdir <PATH>
-  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
-```
-
-### Download and build CTAT
-
-```bash
-nextflow run nf-core/rnafusion/build-ctat.nf \
-  --genome GRCh38 \
-  --outdir <PATH> \
-  --fasta <PATH>/<FASTA \
-  --gtf <PATH>/<GTF>
-```
-
-### Download GRCh37 references
-
-```bash
-# GRCh38 genome assembly is used by default.
-# To use the previous assembly specify it using the --genome flag
-nextflow run nf-core/rnafusion/download-references.nf \
-  --genome GRCh37 \
-  --download_all \
-  --outdir <PATH> \
-  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
-
-# Please note that using the above example command downloads NCBI-based references for STAR-Fusion.
-# To use Ensembl-based references run the following command with the same <PATH> as used above
-
-nextflow run nf-core/rnafusion/build-ctat.nf \
-  --genome GRCh37 \
-  --outdir <PATH> \
-  --fasta <PATH>/<FASTA> \
-  --gtf <PATH>/<GTF>
-```
+> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._
 
-### Tool reference requirements
+## Introduction
 
-| Tool             |        FASTA       |         GTF        |     STAR-index     |     Genome     |       Other       |
-| ---------------- | :----------------: | :----------------: | :----------------: | :------------: | :----------------: |
-| Arriba           | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 | `custom_reference` |
-| EricScript       |         :x:        |         :x:        |         :x:        | GRCh37, GRCh38 | `custom_reference` |
-| FusionCatcher    |         :x:        |         :x:        |         :x:        |     GRCh38     | `custom_reference` |
-| Fusion-Inspector | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |  `ctat_genome_lib` |
-| fusion-report    |         :x:        |         :x:        |         :x:        | GRCh37, GRCh38 |     `databases`    |
-| Pizzly           |         :x:        | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |       `cDNA`       |
-| Squid            |         :x:        | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |          -         |
-| Star-Fusion      | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |  `ctat_genome_lib` |
+<!-- TODO nf-core: Add documentation about anything specific to running your pipeline. For general topics, please point to (and add to) the main nf-core website. -->
 
 ## Running the pipeline
 
-The typical command for running the pipeline is as follows.
+The typical command for running the pipeline is as follows:
 
 ```bash
-nextflow run nf-core/rnafusion \
-  -profile docker \
-  --input "*_R{1,2}.fastq.gz" \
-  --arriba \
-  --star_fusion \
-  --fusioncatcher \
-  --ericscript \
-  --pizzly \
-  --squid \
-  --arriba_vis \
-  --fusion_inspector
+nextflow run nf-core/rnafusion --input '*_R{1,2}.fastq.gz' -profile docker
 ```
 
+This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
+
 Note that the pipeline will create the following files in your working directory:
 
 ```bash
@@ -190,7 +39,7 @@ nextflow pull nf-core/rnafusion
 
 It's a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since.
 
-First, go to the [nf-core/rnafusion releases page](https://github.com/nf-core/rnafusion/releases) and find the latest version number - numeric only (eg. `1.2`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.2`.
+First, go to the [nf-core/rnafusion releases page](https://github.com/nf-core/rnafusion/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`.
 
 This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future.
 
@@ -198,11 +47,11 @@ This version number will be logged in reports when you run the pipeline, so that
 
 > **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen).
 
-### -profile
+### `-profile`
 
 Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments.
 
-Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Conda) - see below.
+Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Conda) - see below.
 
 > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
 
@@ -211,46 +60,44 @@ The pipeline also dynamically loads configurations from [https://github.com/nf-c
 Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important!
 They are loaded in sequence, so later profiles can overwrite earlier profiles.
 
-> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility.
-
-The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time.
-For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation).
-
-Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important!
-They are loaded in sequence, so later profiles can overwrite earlier profiles.
-
 If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended.
 
-- `docker`
-  - A generic configuration profile to be used with [Docker](http://docker.com/)
-  - Pulls software from DockerHub: [`nfcore/rnafusion`](http://hub.docker.com/r/nfcore/rnafusion/)
-- `singularity`
-  - A generic configuration profile to be used with [Singularity](http://singularity.lbl.gov/)
-  - Pulls software from DockerHub: [`nfcore/rnafusion`](http://hub.docker.com/r/nfcore/rnafusion/)
-- `test`
-  - A profile with a complete configuration for automated testing
-  - Includes links to test data so needs no other parameters
-
-### -resume
+* `docker`
+  * A generic configuration profile to be used with [Docker](https://docker.com/)
+  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+* `singularity`
+  * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
+  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+* `podman`
+  * A generic configuration profile to be used with [Podman](https://podman.io/)
+  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+* `conda`
+  * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity or Podman.
+  * A generic configuration profile to be used with [Conda](https://conda.io/docs/)
+  * Pulls most software from [Bioconda](https://bioconda.github.io/)
+* `test`
+  * A profile with a complete configuration for automated testing
+  * Includes links to test data so needs no other parameters
+
+### `-resume`
 
 Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously.
 
 You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names.
 
-### -c
+### `-c`
 
-Specify the path to a specific config file (this is a core Nextflow command).
-See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information.
+Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information.
 
 #### Custom resource requests
 
 Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped.
 
-Whilst these default requirements will hopefully work for most people with most data, you may find that you want to customise the compute resources that the pipeline requests. You can do this by creating a custom config file. For example, to give the workflow process `VEP` 32GB of memory, you could use the following config:
+Whilst these default requirements will hopefully work for most people with most data, you may find that you want to customise the compute resources that the pipeline requests. You can do this by creating a custom config file. For example, to give the workflow process `star` 32GB of memory, you could use the following config:
 
 ```nextflow
 process {
-  withName: VEP {
+  withName: star {
     memory = 32.GB
   }
 }
@@ -258,7 +105,7 @@ process {
 
 See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information.
 
-If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition below). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile.
+If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition above). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile.
 
 If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs).
 
@@ -279,247 +126,3 @@ We recommend adding the following line to your environment to limit this (typica
 ```bash
 NXF_OPTS='-Xms1g -Xmx4g'
 ```
-
-## Pipeline specific arguments
-
-### --input
-
-Use this to specify the location of your input FastQ files. For example:
-
-```bash
---input 'path/to/data/sample_*_{1,2}.fastq.gz'
-```
-
-Please note the following requirements:
-
-1. The path must be enclosed in quotes
-2. The path must have at least one `*` wildcard character
-3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.
-
-If left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`
-
-### --single_end
-
-By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:
-
-```bash
---single_end --input '*.fastq'
-```
-
-### Tool flags
-
-### --arriba
-
-If enabled, executes `Arriba` tool.
-
-- `--arriba_opt`
-  - Specify additional parameters. For more information, please refer to the [documentation](http://arriba.readthedocs.io/en/latest/quickstart/) of the tool.
-
-### --ericscript
-
-If enabled, executes `Ericscript` tool.
-
-- `--ericscript_opt`
-  - Specify additional parameters. For more information, please refer to the [documentation](https://sites.google.com/site/bioericscript/home) of the tool.
-
-### --fusioncatcher
-
-If enabled, executes `Fusioncatcher` tool.
-
-> N.B. that Fusioncatcher is not available when using the `GRCh37` genome assembly.
-
-- `--fusioncatcher_opt`
-  - Specify additional parameters. For more information, please refer to the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md) of the tool.
-
-### --fusion_report
-
-If enabled, download databases for `fusion-report`.
-
-- `fusion_report_opt`
-  - Specify additional parameters. For more information, please refer to the [documentation](https://matq007.github.io/fusion-report/#/) of the tool.
-
-### --pizzly
-
-If enabled, executes `Pizzly` tool.
-
-- `--pizzly_k`
-  - Number of k-mers. Default `31`.
-
-### --squid
-
-If enabled, executes `Squid` tool.
-
-### --star_fusion
-
-If enabled, executes `STAR-Fusion` tool.
-
-- `--star_fusion_opt`
-  - Parameter for specifying additional parameters. For more information, please refer to the [documentation](https://github.com/STAR-Fusion/STAR-Fusion/wiki) of the tool.
-
-## Visualization flags
-
-### --arriba_vis
-
-If enabled, executes build in `Arriba` visualization tool.
-
-### --fusion_inspector
-
-If enabled, executes `Fusion-Inspector` tool.
-
-## Reference genomes
-
-### --arriba_ref
-
-```bash
---arriba_ref '<path to Arriba reference>'
-```
-
-### --databases
-
-Required databases in order to run `fusion-report`.
-
-```bash
---databases '<path to fusion-report databases>'
-```
-
-### --ericscript_ref
-
-Required reference in order to run `EricScript`.
-
-```bash
---ericscript_ref '<path to EricScript reference>'
-```
-
-### --fasta
-
-If you prefer, you can specify the full path to your reference genome when you run the pipeline:
-
-```bash
---fasta '<path to Fasta reference>'
-```
-
-### --fusioncatcher_ref
-
-Required reference in order to run `Fusioncatcher`.
-
-```bash
---fusioncatcher_ref '<path to Fusioncatcher reference>'
-```
-
-### --genome
-
-This pipeline uses `Homo Sapiens` version `GRCh38` by default. Assembly `GRCh37` is optionally available.
-
-> N.B. that using `GRCh37` precludes use of the `Fusioncatcher` tool.
-> Also make sure to specify `--genomes_base`.
-
-```bash
---genome 'GRCh38' --genome_base '</path/to/references>'
-```
-
-### --gtf
-
-Required annotation file.
-
-```bash
---gtf '<path to GTF annotation>'
-```
-
-### --reference_release
-
-Ensembl version.
-
-```bash
-# ftp://ftp.ensembl.org/pub/release-97/fasta/homo_sapiens/
---reference_release '97'
-```
-
-### --star_index
-
-If you prefer, you can specify the full path for `STAR` index when you run the pipeline.
-If not specified, the pipeline will build the index using for reads with length `100bp` (can be adjusted with parameter `--read_length`).
-
-```bash
---star_index '<path to STAR index>'
-```
-
-### --star_fusion_ref
-
-Required reference in order to run `STAR-Fusion`.
-
-```bash
---star_fusion_ref '<path to STAR-Fusion reference>'
-```
-
-### --transcript
-
-Required transcript file.
-
-```bash
---transcript '<path to transcript reference>'
-```
-
-## Other command line parameters
-
-### --debug
-
-To run only a specific tool (testing freshly implemented tool) just add `--debug` parameter.
-This parameter only works on **fusion tools only**!
-
-### --read_length
-
-Length is used to build a STAR index.
-Default is `100bp` (Illumina).
-
-### --outdir
-
-The output directory where the results will be saved.
-
-### --email
-
-Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits.
-If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.
-
-### --email_on_fail
-
-This works exactly as with `--email`, except emails are only sent if the workflow is not successful.
-
-### --max_multiqc_email_size
-
-Threshold size for MultiQC report to be attached in notification email.
-If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB).
-
-### -name
-
-Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic.
-This is used in the MultiQC report (if not default) and in the summary HTML / e-mail (always).
-
-**NB:** Single hyphen (core Nextflow option)
-
-### --custom_config_version
-
-Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.
-
-```bash
-## Download and use config file with following git commid id
---custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96
-```
-
-### --custom_config_base
-
-If you're running offline, nextflow will not be able to fetch the institutional config files
-from the internet. If you don't need them, then this is not a problem. If you do need them,
-you should download the files from the repo and tell nextflow where to find them with the
-`custom_config_base` option. For example:
-
-```bash
-NXF_OPTS='-Xms1g -Xmx4g'
-```
-
-## Job resources
-
-### Automatic resubmission
-
-Each step in the pipeline has a default set of requirements for number of CPUs, memory and time.
-For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original).
-If it still fails after three times then the pipeline is stopped.
diff --git a/download-references.nf b/download-references.nf
deleted file mode 100644
index 4dd34b66..00000000
--- a/download-references.nf
+++ /dev/null
@@ -1,260 +0,0 @@
-#!/usr/bin/env nextflow
-/*
-================================================================================
-                                nf-core/rnafusion
-================================================================================
-nf-core/rnafusion:
- RNA-seq analysis pipeline for detection gene-fusions
---------------------------------------------------------------------------------
- @Homepage
- https://nf-co.re/rnafusion
---------------------------------------------------------------------------------
- @Documentation
- https://nf-co.re/rnafusion/docs
---------------------------------------------------------------------------------
- @Repository
- https://github.com/nf-core/rnafusion
---------------------------------------------------------------------------------
-*/
-
-def helpMessage() {
-    log.info nfcoreHeader()
-    log.info"""
-    Usage:
-
-    The typical command for downloading references is as follows:
-
-    nextflow run nf-core/rnafusion/download-references.nf -profile [PROFILE] [OPTIONS] --outdir /path/to/output
-
-    Mandatory arguments:
-      --outdir [path]               Output directory for downloading
-
-    Options:
-      --download_all [bool]         Download all references
-      --genome [str]                Genome version: GRCh37 or GRCh38 (default)
-      --reference_release [int]     Release number of Ensembl reference for FASTA and GTF
-                                    Default: 97 -> ftp://ftp.ensembl.org/pub/release-97
-      --base [bool]                 Download FASTA, GTF, cDNA
-      --arriba [bool]               Download Arriba references
-      --star_fusion [bool]          Build STAR-Fusion references from FASTA ANF GTF
-      --fusioncatcher [bool]        Download Fusioncatcher references
-      --ericscript [bool]           Download Ericscript references
-      --fusion_report [bool]        Download databases for fusion-report
-      --cosmic_usr [str]            [Required with fusion_report] COSMIC username
-      --cosmic_passwd [str]         [Required with fusion_report] COSMIC password
-    """.stripIndent()
-}
-
-/*
- * SET UP CONFIGURATION VARIABLES
- */
-
-// Show help emssage
-if (params.help) exit 0, helpMessage()
-if (!params.outdir) exit 1, "Output directory not specified!"
-
-running_tools = []
-if (params.base || params.download_all) running_tools.add("Reference v${params.reference_release}")
-if (params.arriba || params.download_all) running_tools.add("Arriba")
-if (params.star_fusion || params.download_all) running_tools.add("STAR-Fusion")
-if (params.fusioncatcher || params.download_all) running_tools.add("Fusioncatcher")
-if (params.ericscript || params.download_all) running_tools.add("Ericscript")
-if (params.fusion_report || params.download_all) {
-    running_tools.add('fusion-report')
-    if (!params.cosmic_usr || !params.cosmic_passwd) exit 1, "Database credentials are required parameter!"
-}
-
-// Header log info
-log.info nfcoreHeader()
-def summary = [:]
-summary['Pipeline Name']  = 'nf-core/rnafusion/download-references.nf'
-summary['Pipeline Version'] = workflow.manifest.version
-summary['References']       = running_tools.size() == 0 ? 'None' : running_tools.join(", ")
-if(workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container"
-summary['Max Resources']    = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job"
-summary['Output dir']   = params.outdir
-summary['User']         = workflow.userName
-log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n")
-log.info "\033[2m----------------------------------------------------\033[0m"
-
-// Check the hostnames against configured profiles
-checkHostname()
-
-/*
-================================================================================
-                                  DOWNLOAD
-================================================================================
-*/
-
-process download_base {
-    publishDir "${params.outdir}/", mode: 'copy'
-
-    when:
-    params.base || params.download_all
-
-    output:
-    file "Homo_sapiens.${params.genome}_r${params.reference_release}.all.fa" into fasta
-    file "Homo_sapiens.${params.genome}_r${params.reference_release}.gtf" into gtf
-    file "Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz" into transcript
-
-    script:
-    def base_url = params.genome == "GRCh37" ? "ftp://ftp.ensembl.org/pub/grch37" : "ftp://ftp.ensembl.org/pub/"
-    """
-    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
-    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
-    gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}_r${params.reference_release}.all.fa
-    wget ${base_url}/release-${params.reference_release}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${params.reference_release}.chr.gtf.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
-    gunzip Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
-    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz
-    """
-}
-
-process download_arriba {
-    publishDir "${params.outdir}/arriba", mode: 'copy'
-
-    when:
-    params.arriba || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    """
-    wget -N https://github.com/suhrig/arriba/releases/download/v1.2.0/arriba_v1.2.0.tar.gz -O arriba_v1.2.0.tar.gz
-    tar -xvzf arriba_v1.2.0.tar.gz && mv arriba_v1.2.0/database/* . && gunzip *.gz && rm -rf arriba_*
-    """
-}
-
-process download_star_fusion {
-    publishDir "${params.outdir}/star-fusion", mode: 'copy'
-
-    when:
-    params.star_fusion || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    """
-    if [[ ${params.genome} == "GRCh37" ]]; then
-      wget -O GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.9/GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz
-      tar -xf GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz --strip-components=5
-      rm GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz
-    else
-      aws s3 --no-sign-request --region ${params.awsregion} cp s3://ngi-igenomes/Homo_sapiens/Ensembl/GRCh38/Genome/CTAT/ctat_star_fusion_1_8_1.tar.gz .
-      tar -xf ctat_star_fusion_1_8_1.tar.gz --strip-components=5
-      rm ctat_star_fusion_1_8_1.tar.gz
-    fi
-    """
-}
-
-process download_fusioncatcher {
-    publishDir "${params.outdir}/fusioncatcher", mode: 'copy'
-
-    when:
-    params.fusioncatcher || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    """
-    if [[ ${params.genome} == "GRCh37" ]]; then
-      echo "FusionCatcher unavailable for GRCh37" > README.txt
-    else
-      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.aa
-      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ab
-      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ac
-      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ad
-      cat human_v98.tar.gz.* | tar xz
-      rm human_v98.tar*
-    fi
-    """
-}
-
-process download_ericscript {
-    publishDir "${params.outdir}/ericscript", mode: 'copy'
-
-    when:
-    params.ericscript || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    def base_url = params.genome == "GRCh37" ? "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIibDRIb0RFdHFlQmM" : "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIiUGt1SnFMZFg4TlE"
-    """
-    if [[ ${params.genome} == "GRCh37" ]]; then
-      gdown ${base_url} ericscript_db_homosapiens_ensembl73.tar.bz2
-    else
-      gdown ${base_url} ericscript_db_homosapiens_ensembl84.tar.bz2
-    fi
-    tar jxf ericscript_db_homosapiens_ensembl*.tar.bz2 && rm ericscript_db_homosapiens_ensembl*.tar.bz2
-    """
-}
-
-process download_databases {
-    publishDir "${params.outdir}/databases", mode: 'copy'
-
-    when:
-    params.fusion_report || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    """
-    fusion_report download --cosmic_usr "${params.cosmic_usr}" --cosmic_passwd "${params.cosmic_passwd}" .
-    """
-}
-
-/*
- * Completion
- */
-workflow.onComplete {
-    log.info "[nf-core/rnafusion/download-references.nf] Pipeline Complete"
-}
-
-def nfcoreHeader() {
-    // Log colors ANSI codes
-    c_black = params.monochrome_logs ? '' : "\033[0;30m";
-    c_blue = params.monochrome_logs ? '' : "\033[0;34m";
-    c_cyan = params.monochrome_logs ? '' : "\033[0;36m";
-    c_dim = params.monochrome_logs ? '' : "\033[2m";
-    c_green = params.monochrome_logs ? '' : "\033[0;32m";
-    c_purple = params.monochrome_logs ? '' : "\033[0;35m";
-    c_reset = params.monochrome_logs ? '' : "\033[0m";
-    c_white = params.monochrome_logs ? '' : "\033[0;37m";
-    c_yellow = params.monochrome_logs ? '' : "\033[0;33m";
-
-    return """    -${c_dim}--------------------------------------------------${c_reset}-
-                                            ${c_green},--.${c_black}/${c_green},-.${c_reset}
-    ${c_blue}        ___     __   __   __   ___     ${c_green}/,-._.--~\'${c_reset}
-    ${c_blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${c_yellow}}  {${c_reset}
-    ${c_blue}  | \\| |       \\__, \\__/ |  \\ |___     ${c_green}\\`-._,-`-,${c_reset}
-                                            ${c_green}`._,._,\'${c_reset}
-    ${c_purple}  nf-core/rnafusion v${workflow.manifest.version}${c_reset}
-    -${c_dim}--------------------------------------------------${c_reset}-
-    """.stripIndent()
-}
-
-def checkHostname() {
-    def c_reset = params.monochrome_logs ? '' : "\033[0m"
-    def c_white = params.monochrome_logs ? '' : "\033[0;37m"
-    def c_red = params.monochrome_logs ? '' : "\033[1;91m"
-    def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m"
-    if (params.hostnames) {
-        def hostname = "hostname".execute().text.trim()
-        params.hostnames.each { prof, hnames ->
-            hnames.each { hname ->
-                if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                    log.error "====================================================\n" +
-                            "  ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" +
-                            "  but your machine hostname is ${c_white}'$hostname'${c_reset}\n" +
-                            "  ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" +
-                            "============================================================"
-                }
-            }
-        }
-    }
-}
diff --git a/environment.yml b/environment.yml
index 4f754e10..162bdacd 100644
--- a/environment.yml
+++ b/environment.yml
@@ -10,16 +10,6 @@ dependencies:
   - conda-forge::markdown=3.1.1
   - conda-forge::pymdown-extensions=6.0
   - conda-forge::pygments=2.5.2
-  # Necessary tools
-  - conda-forge::gdown=3.12.0
+  # TODO nf-core: Add required software dependencies here
   - bioconda::fastqc=0.11.8
   - bioconda::multiqc=1.7
-  # Custom packages
-  - bioconda::star=2.7.1a # has to be for star index
-  - conda-forge::r-data.table=1.12.8
-  - conda-forge::r-gplots=3.0.1.2
-  - bioconda::bioconductor-edger=3.28.0
-  - bioconda::fusion-report=2.1.3
-  # Star-Fusion
-  - conda-forge::awscli=1.18.39
-  - conda-forge::tar=1.32
\ No newline at end of file
diff --git a/main.nf b/main.nf
index cf15754a..5d283978 100644
--- a/main.nf
+++ b/main.nf
@@ -1,23 +1,16 @@
 #!/usr/bin/env nextflow
 /*
-================================================================================
-                                nf-core/rnafusion
-================================================================================
-nf-core/rnafusion:
- RNA-seq analysis pipeline for detection gene-fusions
---------------------------------------------------------------------------------
- @Homepage
- https://nf-co.re/rnafusion
---------------------------------------------------------------------------------
- @Documentation
- https://nf-co.re/rnafusion/docs
---------------------------------------------------------------------------------
- @Repository
+========================================================================================
+                         nf-core/rnafusion
+========================================================================================
+ nf-core/rnafusion Analysis Pipeline.
+ #### Homepage / Documentation
  https://github.com/nf-core/rnafusion
---------------------------------------------------------------------------------
+----------------------------------------------------------------------------------------
 */
 
 def helpMessage() {
+    // TODO nf-core: Add to this help message with new command line parameters
     log.info nfcoreHeader()
     log.info"""
 
@@ -30,45 +23,16 @@ def helpMessage() {
     Mandatory arguments:
       --input [file]                  Path to input data (must be surrounded with quotes)
       -profile [str]                  Configuration profile to use. Can use multiple (comma separated)
-                                      Available: docker, singularity, test, awsbatch, <institute> and more
-      --reference_path [str]          Path to reference folder (includes fasta, gtf, fusion tool ref ...)
-
-    Tool flags:
-      --arriba [bool]                 Run Arriba
-      --arriba_opt [str]              Specify extra parameters for Arriba
-      --ericscript [bool]             Run Ericscript
-      --fusioncatcher [bool]          Run FusionCatcher
-      --fusioncatcher_opt [srt]       Specify extra parameters for FusionCatcher
-      --fusion_report_opt [str]       Specify extra parameters for fusion-report
-      --pizzly [bool]                 Run Pizzly
-      --pizzly_k [int]                Number of k-mers. Deafult 31
-      --squid [bool]                  Run Squid
-      --star_fusion [bool]            Run STAR-Fusion
-      --star_fusion_opt [str]         Specify extra parameters for STAR-Fusion
-
-    Visualization flags:
-      --arriba_vis [bool]             Generate a PDF visualization per detected fusion
-      --fusion_inspector [bool]       Run Fusion-Inspector
-      --fusion_inspector_opt [str]    Specify extra parameters for Fusion-Inspector
-
-    References                        If not specified in the configuration file or you wish to overwrite any of the references.
-      --arriba_ref [file]             Path to Arriba reference
-      --databases [path]              Database path for fusion-report
-      --ericscript_ref [file]         Path to Ericscript reference
-      --fasta [file]                  Path to fasta reference
-      --fusioncatcher_ref [file]      Path to Fusioncatcher reference
-      --gtf [file]                    Path to GTF annotation
-      --star_index [file]             Path to STAR-Index reference
-      --star_fusion_ref [file]        Path to STAR-Fusion reference
-      --transcript [file]             Path to transcript
+                                      Available: conda, docker, singularity, test, awsbatch, <institute> and more
 
     Options:
-      --genome [str]                  Genome version: GRCh37 or GRCh38 (default)
-      --read_length [int]             Length of the reads. Default: 100
+      --genome [str]                  Name of iGenomes reference
       --single_end [bool]             Specifies that the input is single-end reads
 
-    Other Options:
-      --debug [bool]                  Flag to run only specific fusion tool/s and not the whole pipeline. Only works on tool flags.
+    References                        If not specified in the configuration file or you wish to overwrite any of the references
+      --fasta [file]                  Path to fasta reference
+
+    Other options:
       --outdir [file]                 The output directory where the results will be saved
       --publish_dir_mode [str]        Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy)
       --email [email]                 Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits
@@ -83,83 +47,31 @@ def helpMessage() {
     """.stripIndent()
 }
 
-/*
-================================================================================
-                         SET UP CONFIGURATION VARIABLES
-================================================================================
-*/
-
 // Show help message
-if (params.help) exit 0, helpMessage()
-
-running_tools = []
-visualization_tools = []
-reference = [
-    arriba: false,
-    arriba_vis: false,
-    ericscript: false,
-    fusion_inspector: false,
-    fusioncatcher: false,
-    star_fusion: false
-]
-
-// Check if genome exists in the config file
-if (params.genome && !params.genomes.containsKey(params.genome)) {
-    exit 1, "The provided genome '${params.genome}' is not available in the genomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}"
-}
-
-if (!Channel.fromPath(params.genomes_base, checkIfExists: true)) {exit 1, "Directory ${params.genomes_base} doesn't exist."}
-
-params.arriba_ref = params.genome ? params.genomes[params.genome].arriba_ref ?: null : null
-params.databases = params.genome ? params.genomes[params.genome].databases ?: null : null
-params.ericscript_ref = params.genome ? params.genomes[params.genome].ericscript_ref ?: null : null
-params.fasta = params.genome ? params.genomes[params.genome].fasta ?: null : null
-params.fusioncatcher_ref = params.genome ? params.genomes[params.genome].fusioncatcher_ref ?: null : null
-params.gtf = params.genome ? params.genomes[params.genome].gtf ?: null : null
-params.star_fusion_ref = params.genome ? params.genomes[params.genome].star_fusion_ref ?: null : null
-params.transcript = params.genome ? params.genomes[params.genome].transcript ?: null : null
-
-ch_fasta = Channel.value(file(params.fasta)).ifEmpty{exit 1, "Fasta file not found: ${params.fasta}"}
-ch_gtf = Channel.value(file(params.gtf)).ifEmpty{exit 1, "GTF annotation file not found: ${params.gtf}"}
-ch_transcript = Channel.value(file(params.transcript)).ifEmpty{exit 1, "Transcript file not found: ${params.transcript}"}
-
-if (!params.star_index && (!params.fasta && !params.gtf)) exit 1, "Either specify STAR-INDEX or Fasta and GTF!"
-
-if (!params.databases) exit 1, "Database path for fusion-report has to be specified!"
-
-if (params.arriba) {
-    running_tools.add("Arriba")
-    reference.arriba = Channel.value(file(params.arriba_ref)).ifEmpty{exit 1, "Arriba reference directory not found!"}
-}
-
-if (params.arriba_vis) {
-    visualization_tools.add("Arriba")
-    reference.arriba_vis = Channel.value(file(params.arriba_ref)).ifEmpty{exit 1, "Arriba visualization reference directory not found!"}
+if (params.help) {
+    helpMessage()
+    exit 0
 }
 
-if (params.ericscript) {
-    running_tools.add("EricScript")
-    reference.ericscript = Channel.value(file(params.ericscript_ref)).ifEmpty{exit 1, "EricsSript reference not found!"}
-}
-
-if (params.fusioncatcher) {
-    running_tools.add("Fusioncatcher")
-    reference.fusioncatcher = Channel.value(file(params.fusioncatcher_ref)).ifEmpty{exit 1, "Fusioncatcher data directory not found!"}
-}
-
-if (params.fusion_inspector) {
-    visualization_tools.add("Fusion-Inspector")
-    reference.fusion_inspector = Channel.value(file(params.star_fusion_ref)).ifEmpty{exit 1, "Fusion-Inspector reference not found" }
-}
-
-if (params.pizzly) running_tools.add("Pizzly")
+/*
+ * SET UP CONFIGURATION VARIABLES
+ */
 
-if (params.star_fusion) {
-    running_tools.add("STAR-Fusion")
-    reference.star_fusion = Channel.value(file(params.star_fusion_ref)).ifEmpty{exit 1, "Star-Fusion reference directory not found!"}
+// Check if genome exists in the config file
+if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
+    exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}"
 }
 
-if (params.squid) running_tools.add("Squid")
+// TODO nf-core: Add any reference files that are needed
+// Configurable reference genomes
+//
+// NOTE - THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY
+// If you want to use the channel below in a process, define the following:
+//   input:
+//   file fasta from ch_fasta
+//
+params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false
+if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) }
 
 // Has the run name been specified by the user?
 // this has the bonus effect of catching both -name and --name
@@ -188,52 +100,47 @@ ch_output_docs_images = file("$baseDir/docs/images/", checkIfExists: true)
 /*
  * Create a channel for input read files
  */
-if(params.input_paths) {
-    if(params.single_end) {
-        Channel.from(params.input_paths)
-            .map { row -> [ row[0], [file(row[1][0])]] }
-            .ifEmpty{exit 1, "params.input_paths was empty - no input files supplied" }
-            .into{read_files_arriba; read_files_ericscript; ch_read_files_fastqc; read_files_fusion_inspector; read_files_fusioncatcher; read_files_multiqc; read_files_pizzly; read_files_squid; read_files_star_fusion; read_files_summary}
+if (params.input_paths) {
+    if (params.single_end) {
+        Channel
+            .from(params.input_paths)
+            .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] }
+            .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" }
+            .into { ch_read_files_fastqc; ch_read_files_trimming }
     } else {
-        Channel.from(params.input_paths)
-            .map { row -> [ row[0], [file(row[1][0]), file(row[1][1])]] }
-            .ifEmpty{exit 1, "params.input_paths was empty - no input files supplied" }
-            .into{read_files_arriba; read_files_ericscript; ch_read_files_fastqc; read_files_fusion_inspector; read_files_fusioncatcher; read_files_multiqc; read_files_pizzly; read_files_squid; read_files_star_fusion; read_files_summary}
+        Channel
+            .from(params.input_paths)
+            .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] }
+            .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" }
+            .into { ch_read_files_fastqc; ch_read_files_trimming }
     }
 } else {
-    Channel.fromFilePairs( params.input, size: params.single_end ? 1 : 2 )
-        .ifEmpty{exit 1, "Cannot find any reads matching: ${params.input}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." }
-        .into{read_files_arriba; read_files_ericscript; ch_read_files_fastqc; read_files_fusion_inspector; read_files_fusioncatcher; read_files_multiqc; read_files_pizzly; read_files_squid; read_files_star_fusion; read_files_summary}
+    Channel
+        .fromFilePairs(params.input, size: params.single_end ? 1 : 2)
+        .ifEmpty { exit 1, "Cannot find any reads matching: ${params.input}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." }
+        .into { ch_read_files_fastqc; ch_read_files_trimming }
 }
 
-/*
-================================================================================
-                                PRINTING SUMMARY
-================================================================================
-*/
-
 // Header log info
 log.info nfcoreHeader()
 def summary = [:]
 if (workflow.revision) summary['Pipeline Release'] = workflow.revision
-summary['Run Name']            = custom_runName ?: workflow.runName
-summary['Reads']               = params.input
-summary['Fasta Ref']           = params.fasta
-summary['GTF Ref']             = params.gtf
-summary['STAR Index']          = params.star_index ? params.star_index : 'Not specified, building'
-summary['Fusion tools']        = running_tools.size() == 0 ? 'None' : running_tools.join(", ")
-summary['Visualization tools'] = visualization_tools.size() == 0 ? 'None': visualization_tools.join(", ")
+summary['Run Name']         = custom_runName ?: workflow.runName
+// TODO nf-core: Report custom parameters here
+summary['Input']            = params.input
+summary['Fasta Ref']        = params.fasta
 summary['Data Type']        = params.single_end ? 'Single-End' : 'Paired-End'
 summary['Max Resources']    = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job"
-if(workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container"
-summary['Output dir']   = params.outdir
-summary['Launch dir']   = workflow.launchDir
-summary['Working dir']  = workflow.workDir
-summary['Script dir']   = workflow.projectDir
-summary['User']         = workflow.userName
-if(workflow.profile == 'awsbatch') {
-   summary['AWS Region']    = params.awsregion
-   summary['AWS Queue']     = params.awsqueue
+if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container"
+summary['Output dir']       = params.outdir
+summary['Launch dir']       = workflow.launchDir
+summary['Working dir']      = workflow.workDir
+summary['Script dir']       = workflow.projectDir
+summary['User']             = workflow.userName
+if (workflow.profile.contains('awsbatch')) {
+    summary['AWS Region']   = params.awsregion
+    summary['AWS Queue']    = params.awsqueue
+    summary['AWS CLI']      = params.awscli
 }
 summary['Config Profile'] = workflow.profile
 if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description
@@ -267,490 +174,6 @@ Channel.from(summary.collect{ [it.key, it.value] })
     """.stripIndent() }
     .set { ch_workflow_summary }
 
-/*
-================================================================================
-                                  PREPROCESSING
-================================================================================
-*/
-
-/*
- * Build STAR index
- */
-
-process build_star_index {
-    tag "${fasta}-${gtf}"
-    label 'process_medium'
-
-    publishDir params.outdir, mode: 'copy'
-
-    input:
-        file(fasta) from ch_fasta
-        file(gtf) from ch_gtf
-
-    output:
-        file("star-index") into star_index
-
-    when: !(params.star_index)
-
-    script:
-    def avail_mem = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
-    """
-    mkdir star-index
-    STAR \\
-        --runMode genomeGenerate \\
-        --runThreadN ${task.cpus} \\
-        --sjdbGTFfile ${gtf} \\
-        --sjdbOverhang ${params.read_length - 1} \\
-        --genomeDir star-index/ \\
-        --genomeFastaFiles ${fasta} \\
-        ${avail_mem}
-    """
-}
-
-ch_star_index = params.star_index ? Channel.value(file(params.star_index)).ifEmpty{exit 1, "STAR index not found: ${params.star_index}" } : star_index
-
-ch_star_index = ch_star_index.dump(tag:'ch_star_index')
-
-/*
-================================================================================
-                                 FUSION PIPELINE
-================================================================================
-*/
-
-/*
- * Arriba
- */
-process arriba {
-    tag "${sample}"
-    label 'process_medium'
-
-    publishDir "${params.outdir}/tools/Arriba/${sample}", mode: 'copy'
-
-    input:
-        set val(sample), file(reads) from read_files_arriba
-        file(reference) from reference.arriba
-        file(star_index) from ch_star_index
-        file(fasta) from ch_fasta
-        file(gtf) from ch_gtf
-
-    output:
-        set val(sample), file("${sample}_arriba.tsv") optional true into arriba_fusions_summary, arriba_tsv
-        set val(sample), file("${sample}_arriba.bam") optional true into arriba_bam
-        file("*.{tsv,txt}") into arriba_output
-
-    when: params.arriba && (!params.single_end || params.debug)
-
-    script:
-    def extra_params = params.arriba_opt ? params.arriba_opt : ''
-    blacklist = "${reference}/blacklist*${params.genome}*.tsv"
-    """
-    STAR \\
-        --genomeDir ${star_index} \\
-        --runThreadN ${task.cpus} \\
-        --readFilesIn ${reads} \\
-        --outStd BAM_Unsorted \\
-        --outSAMtype BAM Unsorted \\
-        --outSAMunmapped Within \\
-        --outBAMcompression 0 \\
-        --outFilterMultimapNmax 1 \\
-        --outFilterMismatchNmax 3 \\
-        --chimSegmentMin 10 \\
-        --chimOutType WithinBAM SoftClip \\
-        --chimJunctionOverhangMin 10 \\
-        --chimScoreMin 1 \\
-        --chimScoreDropMax 30 \\
-        --chimScoreJunctionNonGTAG 0 \\
-        --chimScoreSeparation 1 \\
-        --alignSJstitchMismatchNmax 5 -1 5 5 \\
-        --chimSegmentReadGapMax 3 \\
-        --readFilesCommand zcat \\
-        --sjdbOverhang ${params.read_length - 1} |
-
-    tee Aligned.out.bam |
-
-    arriba \\
-        -x /dev/stdin \\
-        -a ${fasta} \\
-        -g ${gtf} \\
-        -b ${blacklist} \\
-        -o ${sample}_arriba.tsv -O ${sample}_discarded_arriba.tsv \\
-        -T -P ${extra_params}
-
-    mv Aligned.out.bam ${sample}_arriba.bam
-    """
-}
-
-arriba_fusions_summary = arriba_fusions_summary.dump(tag:'arriba_fusions_summary')
-arriba_visualization = arriba_bam.join(arriba_tsv)
-
-/*
- * STAR-Fusion
- */
-process star_fusion {
-    tag "${sample}"
-    label 'process_high'
-
-    publishDir "${params.outdir}/tools/Star-Fusion/${sample}", mode: 'copy'
-
-    input:
-        set val(sample), file(reads) from read_files_star_fusion
-        file(reference) from reference.star_fusion
-        file(star_index) from ch_star_index
-
-    output:
-        set val(sample), file("${sample}_star-fusion.tsv") optional true into star_fusion_fusions
-        file("*.{tsv,txt}") into star_fusion_output
-
-    when: params.star_fusion || (params.star_fusion && params.debug)
-
-    script:
-    def avail_mem = task.memory ? "--limitBAMsortRAM ${task.memory.toBytes() - 100000000}" : ''
-    option = params.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
-    def extra_params = params.star_fusion_opt ? params.star_fusion_opt : ''
-    """
-    STAR \\
-        --genomeDir ${star_index} \\
-        --readFilesIn ${reads} \\
-        --twopassMode Basic \\
-        --outReadsUnmapped None \\
-        --chimSegmentMin 12 \\
-        --chimJunctionOverhangMin 12 \\
-        --alignSJDBoverhangMin 10 \\
-        --alignMatesGapMax 100000 \\
-        --alignIntronMax 100000 \\
-        --chimSegmentReadGapMax 3 \\
-        --alignSJstitchMismatchNmax 5 -1 5 5 \\
-        --runThreadN ${task.cpus} \\
-        --outSAMstrandField intronMotif ${avail_mem} \\
-        --outSAMunmapped Within \\
-        --outSAMtype BAM Unsorted \\
-        --outSAMattrRGline ID:GRPundef \\
-        --chimMultimapScoreRange 10 \\
-        --chimMultimapNmax 10 \\
-        --chimNonchimScoreDropMin 10 \\
-        --peOverlapNbasesMin 12 \\
-        --peOverlapMMp 0.1 \\
-        --readFilesCommand zcat \\
-        --sjdbOverhang ${params.read_length - 1} \\
-        --chimOutJunctionFormat 1
-
-    STAR-Fusion \\
-        --genome_lib_dir ${reference} \\
-        -J Chimeric.out.junction \\
-        ${option} \\
-        --CPU ${task.cpus} \\
-        --examine_coding_effect \\
-        --output_dir . ${extra_params}
-
-    mv star-fusion.fusion_predictions.tsv ${sample}_star-fusion.tsv
-    mv star-fusion.fusion_predictions.abridged.tsv ${sample}_abridged.tsv
-    mv star-fusion.fusion_predictions.abridged.coding_effect.tsv ${sample}_abridged.coding_effect.tsv
-    """
-}
-
-star_fusion_fusions = star_fusion_fusions.dump(tag:'star_fusion_fusions')
-
-/*
- * Fusioncatcher
- */
-process fusioncatcher {
-    tag "${sample}"
-    label 'process_high'
-
-    publishDir "${params.outdir}/tools/Fusioncatcher/${sample}", mode: 'copy'
-
-    input:
-        set val(sample), file(reads) from read_files_fusioncatcher
-        file(data_dir) from reference.fusioncatcher
-
-    output:
-        set val(sample), file("${sample}_fusioncatcher.txt") optional true into fusioncatcher_fusions
-        file("*.{txt,zip,log}") into fusioncatcher_output
-
-    when: params.genome != "GRCh37" && params.fusioncatcher || (params.fusioncatcher && params.debug)
-
-    script:
-    option = params.single_end ? reads[0] : "${reads[0]},${reads[1]}"
-    def extra_params = params.fusioncatcher_opt ? params.fusioncatcher_opt : ''
-    """
-    fusioncatcher.py \\
-        -d ${data_dir} \\
-        -i ${option} \\
-        --threads ${task.cpus} \\
-        -o . \\
-        --skip-blat ${extra_params}
-
-    mv final-list_candidate-fusion-genes.txt ${sample}_fusioncatcher.txt
-    """
-}
-
-fusioncatcher_fusions = fusioncatcher_fusions.dump(tag:'fusioncatcher_fusions')
-
-/*
- * Ericscript
- */
-process ericscript {
-    tag "${sample}"
-    label 'process_high'
-
-    publishDir "${params.outdir}/tools/EricScript/${sample}", mode: 'copy'
-
-    input:
-        set val(sample), file(reads) from read_files_ericscript
-        file(reference) from reference.ericscript
-
-    output:
-        set val(sample), file("${sample}_ericscript.tsv") optional true into ericscript_fusions
-        set val(sample), file("${sample}_ericscript_total.tsv") optional true into ericscript_output
-
-    when: params.ericscript && (!params.single_end || params.debug)
-
-    script:
-    """
-    ericscript.pl \\
-        -db ${reference} \\
-        -name fusions \\
-        -p ${task.cpus} \\
-        -o ./tmp \\
-        ${reads}
-
-    if [[ -f "tmp/fusions.results.filtered.tsv" ]]; then
-        mv tmp/fusions.results.filtered.tsv ${sample}_ericscript.tsv
-    fi
-
-    if [[ -f "tmp/fusions.results.total.tsv" ]]; then
-        mv tmp/fusions.results.total.tsv ${sample}_ericscript_total.tsv
-    fi
-    """
-}
-
-ericscript_fusions = ericscript_fusions.dump(tag:'ericscript_fusions')
-
-/*
- * Pizzly
- */
-process pizzly {
-    tag "${sample}"
-    label 'process_medium'
-
-    publishDir "${params.outdir}/tools/Pizzly/${sample}", mode: 'copy'
-
-    input:
-        set val(sample), file(reads) from read_files_pizzly
-        file(gtf) from ch_gtf
-        file(transcript) from ch_transcript
-
-    output:
-        set val(sample), file("${sample}_pizzly.txt") optional true into pizzly_fusions
-        file("*.{json,txt}") into pizzly_output
-
-    when: params.pizzly && (!params.single_end || params.debug)
-
-    script:
-    """
-    kallisto index -i index.idx -k ${params.pizzly_k} ${transcript}
-    kallisto quant -t ${task.cpus} -i index.idx --fusion -o output ${reads[0]} ${reads[1]}
-    pizzly -k ${params.pizzly_k} \\
-        --gtf ${gtf} \\
-        --cache index.cache.txt \\
-        --align-score 2 \\
-        --insert-size 400 \\
-        --fasta ${transcript} \\
-        --output pizzly_fusions output/fusion.txt
-    pizzly_flatten_json.py pizzly_fusions.json pizzly_fusions.txt
-
-    mv index.cache.txt ${sample}_pizzly_cache.txt
-    mv pizzly_fusions.json ${sample}_pizzly.txt
-    mv pizzly_fusions.txt ${sample}_pizzly.txt
-    mv pizzly_fusions.unfiltered.json ${sample}_unfiltered_pizzly.json
-    """
-}
-
-pizzly_fusions = pizzly_fusions.dump(tag:'pizzly_fusions')
-
-/*
- * Squid
- */
-process squid {
-    tag "${sample}"
-    label 'process_high'
-
-    publishDir "${params.outdir}/tools/Squid/${sample}", mode: 'copy'
-
-    input:
-        set val(sample), file(reads) from read_files_squid
-        file(star_index) from ch_star_index
-        file(gtf) from ch_gtf
-
-    output:
-        set val(sample), file("${sample}_fusions_annotated.txt") optional true into squid_fusions
-        file("*.txt") into squid_output
-
-    when: params.squid && (!params.single_end || params.debug)
-
-    script:
-    def avail_mem = task.memory ? "--limitBAMsortRAM ${task.memory.toBytes() - 100000000}" : ''
-    """
-    STAR \\
-        --genomeDir ${star_index} \\
-        --sjdbGTFfile ${gtf} \\
-        --runThreadN ${task.cpus} \\
-        --readFilesIn ${reads} \\
-        --twopassMode Basic \\
-        --chimOutType SeparateSAMold \\
-        --chimSegmentMin 20 \\
-        --chimJunctionOverhangMin 12 \\
-        --alignSJDBoverhangMin 10 \\
-        --outReadsUnmapped Fastx \\
-        --outSAMstrandField intronMotif \\
-        --outSAMtype BAM SortedByCoordinate \\
-        ${avail_mem} \\
-        --readFilesCommand zcat
-    mv Aligned.sortedByCoord.out.bam ${sample}Aligned.sortedByCoord.out.bam
-    samtools view -bS Chimeric.out.sam > ${sample}Chimeric.out.bam
-    squid -b ${sample}Aligned.sortedByCoord.out.bam -c ${sample}Chimeric.out.bam -o fusions
-    AnnotateSQUIDOutput.py ${gtf} fusions_sv.txt fusions_annotated.txt
-
-    mv fusions_annotated.txt ${sample}_fusions_annotated.txt
-    """
-}
-
-squid_fusions = squid_fusions.dump(tag:'squid_fusions')
-
-read_files_summary = read_files_summary.dump(tag:'read_files_summary')
-
-files_and_reports_summary = read_files_summary
-    .join(arriba_fusions_summary, remainder: true)
-    .join(ericscript_fusions, remainder: true)
-    .join(fusioncatcher_fusions, remainder: true)
-    .join(pizzly_fusions, remainder: true)
-    .join(squid_fusions, remainder: true)
-    .join(star_fusion_fusions, remainder: true)
-
-files_and_reports_summary = files_and_reports_summary.dump(tag:'files_and_reports_summary')
-
-/*
-================================================================================
-                               SUMMARIZING RESULTS
-================================================================================
-*/
-
-process summary {
-    tag "${sample}"
-
-    publishDir "${params.outdir}/Reports/${sample}", mode: 'copy'
-
-    input:
-        set val(sample), file(reads), file(arriba), file(ericscript), file(fusioncatcher), file(pizzly), file(squid), file(starfusion) from files_and_reports_summary
-
-    output:
-        set val(sample), file("${sample}_fusion_list.tsv") into fusion_inspector_input_list
-        file("${sample}_fusion_genes_mqc.json") into summary_fusions_mq
-        file("*") into report
-
-    when: !params.debug && (running_tools.size() > 0)
-
-
-    script:
-    def extra_params = params.fusion_report_opt ? params.fusion_report_opt : ''
-    def tools = !arriba.empty() ? "--arriba ${arriba} " : ''
-    tools += !ericscript.empty() ? "--ericscript ${ericscript} " : ''
-    tools += !fusioncatcher.empty() ? "--fusioncatcher ${fusioncatcher} " : ''
-    tools += !pizzly.empty() ? "--pizzly ${pizzly} " : ''
-    tools += !squid.empty() ? "--squid ${squid} " : ''
-    tools += !starfusion.empty() ? "--starfusion ${starfusion} " : ''
-    """
-    fusion_report run ${sample} . ${params.databases} \\
-        ${tools} ${extra_params}
-    mv fusion_list.tsv ${sample}_fusion_list.tsv
-    mv fusion_genes_mqc.json ${sample}_fusion_genes_mqc.json
-    tar -czf ${sample}_fusion-report.tar.gz --exclude=${sample}* ./*
-    """
-}
-
-/*************************************************************
- * Visualization
- ************************************************************/
-
-/*
- * Arriba Visualization
- */
-process arriba_visualization {
-    tag "${sample}"
-    label 'process_medium'
-
-    publishDir "${params.outdir}/tools/Arriba/${sample}", mode: 'copy'
-
-    input:
-        file(reference) from reference.arriba_vis
-        set sample, file(bam), file(fusions) from arriba_visualization
-        file(gtf) from ch_gtf
-
-    output:
-        file("${sample}.pdf") optional true into arriba_visualization_output
-
-    when: params.arriba_vis && (!params.single_end || params.debug)
-
-    script:
-    def suff_mem = ("${(task.memory.toBytes() - 6000000000) / task.cpus}" > 2000000000) ? 'true' : 'false'
-    def avail_mem = (task.memory && suff_mem) ? "-m" + "${(task.memory.toBytes() - 6000000000) / task.cpus}" : ''
-    cytobands = "${reference}/cytobands*${params.genome}*.tsv"
-    proteinDomains = "${reference}/protein_domains*${params.genome}*.gff3"
-    """
-    samtools sort -@ ${task.cpus} ${avail_mem} -O bam ${bam} > Aligned.sortedByCoord.out.bam
-    samtools index Aligned.sortedByCoord.out.bam
-    draw_fusions.R \\
-        --fusions=${fusions} \\
-        --alignments=Aligned.sortedByCoord.out.bam \\
-        --output=${sample}.pdf \\
-        --annotation=${gtf} \\
-        --cytobands=\$(echo ${cytobands}) \\
-        --proteinDomains=\$(echo ${proteinDomains})
-    """
-}
-
-
-fusion_inspector_input = fusion_inspector_input_list.join(read_files_fusion_inspector)
-
-fusion_inspector_input = fusion_inspector_input.dump(tag:'fusion_inspector_input')
-
-/*
- * Fusion Inspector
- */
-process fusion_inspector {
-    tag "${sample}"
-    label 'process_high'
-
-    publishDir "${params.outdir}/tools/FusionInspector/${sample}", mode: 'copy'
-
-    input:
-        set val(sample), file(fi_input_list), file(reads) from fusion_inspector_input
-        file(reference) from reference.fusion_inspector
-
-    output:
-        file("*.{fa,gtf,bed,bam,bai,txt,html}") into fusion_inspector_output
-
-    when: params.fusion_inspector && (!params.single_end || params.debug)
-
-    script:
-    def extra_params = params.fusion_inspector_opt ? params.fusion_inspector_opt : ''
-    """
-    FusionInspector \\
-        --fusions ${fi_input_list} \\
-        --genome_lib ${reference} \\
-        --left_fq ${reads[0]} \\
-        --right_fq ${reads[1]} \\
-        --CPU ${task.cpus} \\
-        -O . \\
-        --out_prefix finspector \\
-        --vis ${extra_params}
-    """
-}
-
-/*************************************************************
- * Quality check & software verions
- ************************************************************/
-
 /*
  * Parse software version numbers
  */
@@ -766,25 +189,18 @@ process get_software_versions {
     file "software_versions.csv"
 
     script:
+    // TODO nf-core: Get all tools to print their version number here
     """
-    echo ${workflow.manifest.version} > v_pipeline.txt
-    echo ${workflow.nextflow.version} > v_nextflow.txt
+    echo $workflow.manifest.version > v_pipeline.txt
+    echo $workflow.nextflow.version > v_nextflow.txt
     fastqc --version > v_fastqc.txt
     multiqc --version > v_multiqc.txt
-    cat ${baseDir}/containers/arriba/environment.yml > v_arriba.txt
-    cat ${baseDir}/containers/fusioncatcher/environment.yml > v_fusioncatcher.txt
-    cat ${baseDir}/containers/star-fusion/environment.yml > v_fusion_inspector.txt
-    cat ${baseDir}/containers/star-fusion/environment.yml > v_star_fusion.txt
-    cat ${baseDir}/containers/ericscript/environment.yml > v_ericscript.txt
-    cat ${baseDir}/containers/pizzly/environment.yml > v_pizzly.txt
-    cat ${baseDir}/containers/squid/environment.yml > v_squid.txt
-    cat ${baseDir}/environment.yml > v_fusion_report.txt
     scrape_software_versions.py &> software_versions_mqc.yaml
     """
 }
 
 /*
- * FastQC
+ * STEP 1 - FastQC
  */
 process fastqc {
     tag "$name"
@@ -800,8 +216,6 @@ process fastqc {
     output:
     file "*_fastqc.{zip,html}" into ch_fastqc_results
 
-    when: !params.debug
-
     script:
     """
     fastqc --quiet --threads $task.cpus $reads
@@ -809,7 +223,7 @@ process fastqc {
 }
 
 /*
- * MultiQC
+ * STEP 2 - MultiQC
  */
 process multiqc {
     publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode
@@ -817,9 +231,9 @@ process multiqc {
     input:
     file (multiqc_config) from ch_multiqc_config
     file (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([])
+    // TODO nf-core: Add in log files from your new processes for MultiQC to find!
     file ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([])
     file ('software_versions/*') from ch_software_versions_yaml.collect()
-    file (fusions_mq) from summary_fusions_mq.collect().ifEmpty([])
     file workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml")
 
     output:
@@ -827,31 +241,28 @@ process multiqc {
     file "*_data"
     file "multiqc_plots"
 
-    when: !params.debug
-
     script:
     rtitle = custom_runName ? "--title \"$custom_runName\"" : ''
     rfilename = custom_runName ? "--filename " + custom_runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" : ''
     custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : ''
+    // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time
     """
     multiqc -f $rtitle $rfilename $custom_config_file .
     """
 }
 
 /*
- * Output Description HTML
+ * STEP 3 - Output Description HTML
  */
 process output_documentation {
     publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode
 
     input:
-        file output_docs from ch_output_docs
-        file images from ch_output_docs_images
+    file output_docs from ch_output_docs
+    file images from ch_output_docs_images
 
     output:
-        file("results_description.html")
-
-    when: !params.debug
+    file "results_description.html"
 
     script:
     """
@@ -892,6 +303,8 @@ workflow.onComplete {
     email_fields['summary']['Nextflow Build'] = workflow.nextflow.build
     email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
 
+    // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size)
+    // On success try attach the multiqc report
     def mqc_report = null
     try {
         if (workflow.success) {
@@ -913,12 +326,12 @@ workflow.onComplete {
 
     // Render the TXT template
     def engine = new groovy.text.GStringTemplateEngine()
-    def tf = new File("${baseDir}/assets/email_template.txt")
+    def tf = new File("$baseDir/assets/email_template.txt")
     def txt_template = engine.createTemplate(tf).make(email_fields)
     def email_txt = txt_template.toString()
 
     // Render the HTML template
-    def hf = new File("${baseDir}/assets/email_template.html")
+    def hf = new File("$baseDir/assets/email_template.html")
     def html_template = engine.createTemplate(hf).make(email_fields)
     def email_html = html_template.toString()
 
@@ -976,6 +389,7 @@ workflow.onComplete {
 
 }
 
+
 def nfcoreHeader() {
     // Log colors ANSI codes
     c_black = params.monochrome_logs ? '' : "\033[0;30m";
diff --git a/nextflow.config b/nextflow.config
index 04a2092c..9c2a32fe 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -7,59 +7,12 @@
 
 // Global default params, used in configs
 params {
-  genome = 'GRCh38'
-  genomes_base = false
-  reference_release = '97'
 
-  // Options: Building STAR-star_index
-  star_index = false
-  read_length = 100
-
-  // Fusion tools
-  arriba = false
-  star_fusion = false
-  fusioncatcher = false
-  fusion_inspector = false
-  ericscript = false
-  pizzly = false
-  squid = false
-
-  // Options: Arriba
-  arriba_opt = false
-  arriba_vis = false
-
-  // Options: STAR-Fusion
-  star_fusion_opt = false
-
-  // Options: FusionCatcher
-  fusioncatcher_opt = false
-  
-  // Options: Pizzly
-  pizzly_k = 31
-
-  // Options: Fusion-Inspector
-  fusion_inspector_opt = false
-
-  // Options: fusion-report
-  fusion_report_opt = false
-
-  // Defaults
+  // Workflow flags
+  // TODO nf-core: Specify your pipeline's command line flags
+  genome = false
   input = "data/*{1,2}.fastq.gz"
   single_end = false
-  clusterOptions = false
-  awsqueue = false
-  awsregion = 'eu-west-1'
-  input_paths = null
-  debug = false
-
-  // Options: download-references.nf
-  base = false
-  download_all = false
-  fusion_report = false
-  cosmic_usr = false
-  cosmic_passwd = false
-
-  // Shared default variables across different scripts
   outdir = './results'
   publish_dir_mode = 'copy'
 
@@ -72,7 +25,9 @@ params {
   plaintext_email = false
   monochrome_logs = false
   help = false
+  igenomes_base = 's3://ngi-igenomes/igenomes/'
   tracedir = "${params.outdir}/pipeline_info"
+  igenomes_ignore = false
   custom_config_version = 'master'
   custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
   hostnames = false
@@ -84,12 +39,16 @@ params {
   max_memory = 128.GB
   max_cpus = 16
   max_time = 240.h
+
 }
 
 // Container slug. Stable releases should specify release tag!
 // Developmental code should specify :dev
 process.container = 'nfcore/rnafusion:dev'
 
+// Load base.config by default for all pipelines
+includeConfig 'conf/base.config'
+
 // Load nf-core custom profiles from different Institutions
 try {
   includeConfig "${params.custom_config_base}/nfcore_custom.config"
@@ -97,19 +56,6 @@ try {
   System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
 }
 
-// Load nf-core/rnafusion custom profiles from different Institutions
-try {
-  includeConfig "${params.custom_config_base}/pipeline/rnafusion.config"
-} catch (Exception e) {
-  System.err.println("WARNING: Could not load nf-core/config/rnafusion profiles: ${params.custom_config_base}/pipeline/rnafusion.config")
-}
-
-// Load base.config by default for all pipelines
-includeConfig 'conf/base.config'
-
-// Load genomes.config
-includeConfig 'conf/genomes.config'
-
 profiles {
   conda { process.conda = "$baseDir/environment.yml" }
   debug { process.beforeScript = 'echo $HOSTNAME' }
@@ -125,9 +71,17 @@ profiles {
     singularity.enabled = true
     singularity.autoMounts = true
   }
+  podman {
+    podman.enabled = true
+  }
   test { includeConfig 'conf/test.config' }
 }
 
+// Load igenomes.config if required
+if (!params.igenomes_ignore) {
+  includeConfig 'conf/igenomes.config'
+}
+
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container
 env {
   PYTHONNOUSERSITE = 1
@@ -196,4 +150,4 @@ def check_max(obj, type) {
       return obj
     }
   }
-}
\ No newline at end of file
+}
diff --git a/nextflow_schema.json b/nextflow_schema.json
index a87cf4ad..1a615348 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -1,5 +1,5 @@
 {
-    "$schema": "https://json-schema.org/draft-07/schema",
+    "$schema": "http://json-schema.org/draft-07/schema",
     "$id": "https://raw.githubusercontent.com/nf-core/rnafusion/master/nextflow_schema.json",
     "title": "nf-core/rnafusion pipeline parameters",
     "description": "Nextflow rnafusion analysis pipeline, part of the nf-core community.",
@@ -59,39 +59,19 @@
                     "description": "Path to FASTA genome file.",
                     "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible."
                 },
-                "gtf": {
-                    "type": "string"
-                },
-                "genomes_base": {
-                    "type": "string"
-                },
-                "reference_release": {
-                    "type": "integer",
-                    "default": 97
-                },
-                "star_index": {
-                    "type": "string"
-                },
-                "transcript": {
-                    "type": "string"
-                },
-                "base": {
-                    "type": "string"
-                },
-                "arriba_ref": {
-                    "type": "string"
-                },
-                "databases": {
-                    "type": "string"
-                },
-                "ericscript_ref": {
-                    "type": "string"
-                },
-                "fusioncatcher_ref": {
-                    "type": "string"
+                "igenomes_base": {
+                    "type": "string",
+                    "description": "Directory / URL base for iGenomes references.",
+                    "default": "s3://ngi-igenomes/igenomes/",
+                    "fa_icon": "fas fa-cloud-download-alt",
+                    "hidden": true
                 },
-                "star_fusion_ref": {
-                    "type": "string"
+                "igenomes_ignore": {
+                    "type": "boolean",
+                    "description": "Do not load the iGenomes reference config.",
+                    "fa_icon": "fas fa-ban",
+                    "hidden": true,
+                    "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
                 }
             }
         },
@@ -121,7 +101,7 @@
                         "link",
                         "copy",
                         "copyNoFollow",
-                        "mov"
+                        "move"
                     ]
                 },
                 "name": {
@@ -176,90 +156,6 @@
                 }
             }
         },
-        "new_group_1": {
-            "title": "New Group 1",
-            "type": "object",
-            "description": "",
-            "default": "",
-            "properties": {
-                "arriba": {
-                    "type": "string"
-                },
-                "read_length": {
-                    "type": "integer",
-                    "default": 100
-                },
-                "star_fusion": {
-                    "type": "string"
-                },
-                "fusioncatcher": {
-                    "type": "string"
-                },
-                "fusion_inspector": {
-                    "type": "string"
-                },
-                "ericscript": {
-                    "type": "string"
-                },
-                "pizzly": {
-                    "type": "string"
-                },
-                "squid": {
-                    "type": "string"
-                },
-                "arriba_opt": {
-                    "type": "string"
-                },
-                "arriba_vis": {
-                    "type": "string"
-                },
-                "star_fusion_opt": {
-                    "type": "string"
-                },
-                "fusioncatcher_opt": {
-                    "type": "string"
-                },
-                "pizzly_k": {
-                    "type": "integer",
-                    "default": 31
-                },
-                "fusion_inspector_opt": {
-                    "type": "string"
-                },
-                "fusion_report_opt": {
-                    "type": "string"
-                },
-                "fusion_report": {
-                    "type": "string"
-                },
-                "input_paths": {
-                    "type": "string",
-                    "default": "null",
-                    "hidden": true
-                },
-                "debug": {
-                    "type": "string",
-                    "hidden": true
-                }
-            }
-        },
-        "new_group_2": {
-            "title": "New Group 2",
-            "type": "object",
-            "description": "",
-            "default": "",
-            "properties": {
-                "download_all": {
-                    "type": "string"
-                },
-                "cosmic_usr": {
-                    "type": "string"
-                },
-                "cosmic_passwd": {
-                    "type": "string"
-                }
-            }
-        },
         "max_job_request_options": {
             "title": "Max job request options",
             "type": "object",
@@ -339,17 +235,6 @@
                     "description": "Institutional config URL link.",
                     "hidden": true,
                     "fa_icon": "fas fa-users-cog"
-                },
-                "clusterOptions": {
-                    "type": "string",
-                    "hidden": true
-                },
-                "awsqueue": {
-                    "type": "string"
-                },
-                "awsregion": {
-                    "type": "string",
-                    "default": "eu-west-1"
                 }
             }
         }
@@ -364,12 +249,6 @@
         {
             "$ref": "#/definitions/generic_options"
         },
-        {
-            "$ref": "#/definitions/new_group_1"
-        },
-        {
-            "$ref": "#/definitions/new_group_2"
-        },
         {
             "$ref": "#/definitions/max_job_request_options"
         },
diff --git a/scripts/build.sh b/scripts/build.sh
deleted file mode 100755
index 5c439c43..00000000
--- a/scripts/build.sh
+++ /dev/null
@@ -1,51 +0,0 @@
-#!/bin/bash
-
-PREFIX="nfcore/rnafusion"
-
-create_container() {
-    TOOL_PATH=$1
-    VERSION="$(cat $TOOL_PATH/environment.yml | grep "name:" | cut -d":" -f2 | cut -d "_" -f2)"
-    TOOL_NAME=`basename $TOOL_PATH`
-    CONTAINER_NAME="${PREFIX}:${TOOL_NAME}_${VERSION}"
-    echo "Building [$CONTAINER_NAME]"
-    docker build $TOOL_PATH -t $CONTAINER_NAME
-    docker push $CONTAINER_NAME
-}
-
-if [ $# -eq 0 ]; then
-    echo "No tool name specified!"
-    echo "Run scripts/build.sh -h for help"
-    exit 1
-fi
-
-if [ $1 == "-h" ]; then
-    echo "Utility for building docker containers from tools/"
-    echo "Usage: scripts/build.sh [options]"
-    echo 
-    echo "Options:"
-    echo "  all             build all tools including main image"
-    echo "  <tool name>     builds specific tool"
-    echo "                  Example: sh scripts/build.sh ericscript"
-    exit 0
-fi
-
-if [ $1 == "all" ]; then
-    for TOOL in containers/*/; do
-        create_container `pwd`/$TOOL ${TOOL%?}
-    done
-    # Build main container
-    VERSION="$(cat nextflow.config | grep -m1 "container" | cut -d":" -f2 | cut -d "'" -f1)"
-    CONTAINER_NAME=$PREFIX:$VERSION
-    echo "Building [$CONTAINER_NAME]"
-    docker build . -t $CONTAINER_NAME
-    docker push $CONTAINER_NAME
-else
-    TOOL=$1
-    TOOL_PATH="$(pwd)/containers/$TOOL"
-    if [ ! -d $TOOL_PATH ]; then
-        echo "The tool doesn't exist"
-        exit 1
-    else
-        create_container $TOOL_PATH
-    fi
-fi

From f2004bb0088d0d3ccd38cea56b4a5bd8ce2eceaa Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 19 Nov 2020 13:47:45 +0000
Subject: [PATCH 016/359] Template update for nf-core/tools version 1.12

---
 .github/ISSUE_TEMPLATE/bug_report.md          |  7 ++++-
 .github/ISSUE_TEMPLATE/config.yml             |  8 +++++
 .github/ISSUE_TEMPLATE/feature_request.md     |  6 ++++
 .github/workflows/awsfulltest.yml             |  7 +++--
 .github/workflows/awstest.yml                 |  2 +-
 .github/workflows/branch.yml                  |  2 +-
 .github/workflows/ci.yml                      | 12 ++++----
 .github/workflows/linting.yml                 | 13 +++++++--
 .github/workflows/linting_comment.yml         | 29 +++++++++++++++++++
 .github/workflows/push_dockerhub_dev.yml      | 28 ++++++++++++++++++
 ...ckerhub.yml => push_dockerhub_release.yml} | 15 ++--------
 Dockerfile                                    |  2 +-
 README.md                                     |  2 +-
 assets/sendmail_template.txt                  |  2 +-
 main.nf                                       | 14 ++++-----
 nextflow.config                               |  5 ++--
 16 files changed, 116 insertions(+), 38 deletions(-)
 create mode 100644 .github/ISSUE_TEMPLATE/config.yml
 create mode 100644 .github/workflows/linting_comment.yml
 create mode 100644 .github/workflows/push_dockerhub_dev.yml
 rename .github/workflows/{push_dockerhub.yml => push_dockerhub_release.yml} (68%)

diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md
index 09c1d9d4..371eaa38 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.md
+++ b/.github/ISSUE_TEMPLATE/bug_report.md
@@ -1,10 +1,15 @@
+---
+name: Bug report
+about: Report something that is broken or incorrect
+labels: bug
+---
+
 <!--
 # nf-core/rnafusion bug report
 
 Hi there!
 
 Thanks for telling us about a problem with the pipeline.
-
 Please delete this text and anything that's not relevant from the template below:
 -->
 
diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml
new file mode 100644
index 00000000..1fccc741
--- /dev/null
+++ b/.github/ISSUE_TEMPLATE/config.yml
@@ -0,0 +1,8 @@
+blank_issues_enabled: false
+contact_links:
+  - name: Join nf-core
+    url: https://nf-co.re/join
+    about: Please join the nf-core community here
+  - name: "Slack #rnafusion channel"
+    url: https://nfcore.slack.com/channels/rnafusion
+    about: Discussion about the nf-core/rnafusion pipeline
diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md
index 4560071e..8bad5163 100644
--- a/.github/ISSUE_TEMPLATE/feature_request.md
+++ b/.github/ISSUE_TEMPLATE/feature_request.md
@@ -1,3 +1,9 @@
+---
+name: Feature request
+about: Suggest an idea for the nf-core website
+labels: enhancement
+---
+
 <!--
 # nf-core/rnafusion feature request
 
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 214d01aa..eb4441e2 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -4,8 +4,9 @@ name: nf-core AWS full size tests
 # It runs the -profile 'test_full' on AWS batch
 
 on:
-  release:
-    types: [published]
+  workflow_run:
+    workflows: ["nf-core Docker push (release)"]
+    types: [completed]
   workflow_dispatch:
 
 jobs:
@@ -15,7 +16,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Setup Miniconda
-        uses: goanpeca/setup-miniconda@v1.0.2
+        uses: conda-incubator/setup-miniconda@v2
         with:
           auto-update-conda: true
           python-version: 3.7
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index e54f10f7..9829a938 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -13,7 +13,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Setup Miniconda
-        uses: goanpeca/setup-miniconda@v1.0.2
+        uses: conda-incubator/setup-miniconda@v2
         with:
           auto-update-conda: true
           python-version: 3.7
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index eda35177..51392d1a 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -2,7 +2,7 @@ name: nf-core branch protection
 # This workflow is triggered on PRs to master branch on the repository
 # It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev`
 on:
-  pull_request:
+  pull_request_target:
     branches: [master]
 
 jobs:
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index abcbc3b0..5b4e75cd 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -20,29 +20,31 @@ jobs:
     strategy:
       matrix:
         # Nextflow versions: check pipeline minimum and current latest
-        nxf_ver: ['19.10.0', '']
+        nxf_ver: ['20.04.0', '']
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
 
       - name: Check if Dockerfile or Conda environment changed
-        uses: technote-space/get-diff-action@v1
+        uses: technote-space/get-diff-action@v4
         with:
-          PREFIX_FILTER: |
+          FILES: |
             Dockerfile
             environment.yml
 
       - name: Build new docker image
-        if: env.GIT_DIFF
+        if: env.MATCHED_FILES
         run: docker build --no-cache . -t nfcore/rnafusion:dev
 
       - name: Pull docker image
-        if: ${{ !env.GIT_DIFF }}
+        if: ${{ !env.MATCHED_FILES }}
         run: |
           docker pull nfcore/rnafusion:dev
           docker tag nfcore/rnafusion:dev nfcore/rnafusion:dev
 
       - name: Install Nextflow
+        env:
+          CAPSULE_LOG: none
         run: |
           wget -qO- get.nextflow.io | bash
           sudo mv nextflow /usr/local/bin/
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 8e8d5bbc..bef81e61 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -38,6 +38,8 @@ jobs:
         uses: actions/checkout@v2
 
       - name: Install Nextflow
+        env:
+          CAPSULE_LOG: none
         run: |
           wget -qO- get.nextflow.io | bash
           sudo mv nextflow /usr/local/bin/
@@ -57,12 +59,19 @@ jobs:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
-        run: nf-core -l lint_log.txt lint ${GITHUB_WORKSPACE}
+        run: nf-core -l lint_log.txt lint ${GITHUB_WORKSPACE} --markdown lint_results.md
+
+      - name: Save PR number
+        if: ${{ always() }}
+        run: echo ${{ github.event.pull_request.number }} > PR_number.txt
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
         uses: actions/upload-artifact@v2
         with:
           name: linting-log-file
-          path: lint_log.txt
+          path: |
+            lint_log.txt
+            lint_results.md
+            PR_number.txt
 
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
new file mode 100644
index 00000000..90f03c6f
--- /dev/null
+++ b/.github/workflows/linting_comment.yml
@@ -0,0 +1,29 @@
+
+name: nf-core linting comment
+# This workflow is triggered after the linting action is complete
+# It posts an automated comment to the PR, even if the PR is coming from a fork
+
+on:
+  workflow_run:
+    workflows: ["nf-core linting"]
+
+jobs:
+  test:
+    runs-on: ubuntu-latest
+    steps:
+      - name: Download lint results
+        uses: dawidd6/action-download-artifact@v2
+        with:
+          workflow: linting.yml
+
+      - name: Get PR number
+        id: pr_number
+        run: echo "::set-output name=pr_number::$(cat linting-logs/PR_number.txt)"
+
+      - name: Post PR comment
+        uses: marocchino/sticky-pull-request-comment@v2
+        with:
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+          number: ${{ steps.pr_number.outputs.pr_number }}
+          path: linting-logs/lint_results.md
+
diff --git a/.github/workflows/push_dockerhub_dev.yml b/.github/workflows/push_dockerhub_dev.yml
new file mode 100644
index 00000000..b970cb61
--- /dev/null
+++ b/.github/workflows/push_dockerhub_dev.yml
@@ -0,0 +1,28 @@
+name: nf-core Docker push (dev)
+# This builds the docker image and pushes it to DockerHub
+# Runs on nf-core repo releases and push event to 'dev' branch (PR merges)
+on:
+  push:
+    branches:
+      - dev
+
+jobs:
+  push_dockerhub:
+    name: Push new Docker image to Docker Hub (dev)
+    runs-on: ubuntu-latest
+    # Only run for the nf-core repo, for releases and merged PRs
+    if: ${{ github.repository == 'nf-core/rnafusion' }}
+    env:
+      DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }}
+      DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }}
+    steps:
+      - name: Check out pipeline code
+        uses: actions/checkout@v2
+
+      - name: Build new docker image
+        run: docker build --no-cache . -t nfcore/rnafusion:dev
+
+      - name: Push Docker image to DockerHub (dev)
+        run: |
+          echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
+          docker push nfcore/rnafusion:dev
diff --git a/.github/workflows/push_dockerhub.yml b/.github/workflows/push_dockerhub_release.yml
similarity index 68%
rename from .github/workflows/push_dockerhub.yml
rename to .github/workflows/push_dockerhub_release.yml
index 44440ece..85b08e61 100644
--- a/.github/workflows/push_dockerhub.yml
+++ b/.github/workflows/push_dockerhub_release.yml
@@ -1,16 +1,13 @@
-name: nf-core Docker push
+name: nf-core Docker push (release)
 # This builds the docker image and pushes it to DockerHub
 # Runs on nf-core repo releases and push event to 'dev' branch (PR merges)
 on:
-  push:
-    branches:
-      - dev
   release:
     types: [published]
 
 jobs:
   push_dockerhub:
-    name: Push new Docker image to Docker Hub
+    name: Push new Docker image to Docker Hub (release)
     runs-on: ubuntu-latest
     # Only run for the nf-core repo, for releases and merged PRs
     if: ${{ github.repository == 'nf-core/rnafusion' }}
@@ -24,15 +21,7 @@ jobs:
       - name: Build new docker image
         run: docker build --no-cache . -t nfcore/rnafusion:latest
 
-      - name: Push Docker image to DockerHub (dev)
-        if: ${{ github.event_name == 'push' }}
-        run: |
-          echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
-          docker tag nfcore/rnafusion:latest nfcore/rnafusion:dev
-          docker push nfcore/rnafusion:dev
-
       - name: Push Docker image to DockerHub (release)
-        if: ${{ github.event_name == 'release' }}
         run: |
           echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
           docker push nfcore/rnafusion:latest
diff --git a/Dockerfile b/Dockerfile
index deb83220..1138a469 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.11
+FROM nfcore/base:1.12
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 
diff --git a/README.md b/README.md
index 09cf24b9..c0399601 100644
--- a/README.md
+++ b/README.md
@@ -4,7 +4,7 @@
 
 [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions)
-[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A519.10.0-brightgreen.svg)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A520.04.0-brightgreen.svg)](https://www.nextflow.io/)
 
 [![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/)
 [![Docker](https://img.shields.io/docker/automated/nfcore/rnafusion.svg)](https://hub.docker.com/r/nfcore/rnafusion)
diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt
index 3b09baa6..db67ce18 100644
--- a/assets/sendmail_template.txt
+++ b/assets/sendmail_template.txt
@@ -14,7 +14,7 @@ Content-Transfer-Encoding: base64
 Content-ID: <nfcorepipelinelogo>
 Content-Disposition: inline; filename="nf-core-rnafusion_logo.png"
 
-<% out << new File("$baseDir/assets/nf-core-rnafusion_logo.png").
+<% out << new File("$projectDir/assets/nf-core-rnafusion_logo.png").
   bytes.
   encodeBase64().
   toString().
diff --git a/main.nf b/main.nf
index 5d283978..15b16621 100644
--- a/main.nf
+++ b/main.nf
@@ -92,10 +92,10 @@ if (workflow.profile.contains('awsbatch')) {
 }
 
 // Stage config files
-ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true)
+ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true)
 ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty()
-ch_output_docs = file("$baseDir/docs/output.md", checkIfExists: true)
-ch_output_docs_images = file("$baseDir/docs/images/", checkIfExists: true)
+ch_output_docs = file("$projectDir/docs/output.md", checkIfExists: true)
+ch_output_docs_images = file("$projectDir/docs/images/", checkIfExists: true)
 
 /*
  * Create a channel for input read files
@@ -326,18 +326,18 @@ workflow.onComplete {
 
     // Render the TXT template
     def engine = new groovy.text.GStringTemplateEngine()
-    def tf = new File("$baseDir/assets/email_template.txt")
+    def tf = new File("$projectDir/assets/email_template.txt")
     def txt_template = engine.createTemplate(tf).make(email_fields)
     def email_txt = txt_template.toString()
 
     // Render the HTML template
-    def hf = new File("$baseDir/assets/email_template.html")
+    def hf = new File("$projectDir/assets/email_template.html")
     def html_template = engine.createTemplate(hf).make(email_fields)
     def email_html = html_template.toString()
 
     // Render the sendmail template
-    def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ]
-    def sf = new File("$baseDir/assets/sendmail_template.txt")
+    def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ]
+    def sf = new File("$projectDir/assets/sendmail_template.txt")
     def sendmail_template = engine.createTemplate(sf).make(smail_fields)
     def sendmail_html = sendmail_template.toString()
 
diff --git a/nextflow.config b/nextflow.config
index 9c2a32fe..295d2f33 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -57,7 +57,7 @@ try {
 }
 
 profiles {
-  conda { process.conda = "$baseDir/environment.yml" }
+  conda { process.conda = "$projectDir/environment.yml" }
   debug { process.beforeScript = 'echo $HOSTNAME' }
   docker {
     docker.enabled = true
@@ -75,6 +75,7 @@ profiles {
     podman.enabled = true
   }
   test { includeConfig 'conf/test.config' }
+  test_full { includeConfig 'conf/test_full.config' }
 }
 
 // Load igenomes.config if required
@@ -115,7 +116,7 @@ manifest {
   homePage = 'https://github.com/nf-core/rnafusion'
   description = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
   mainScript = 'main.nf'
-  nextflowVersion = '>=19.10.0'
+  nextflowVersion = '>=20.04.0'
   version = '1.3.0dev'
 }
 

From 1f1cb183df3c3e107df33b064b506c0e6a3212b9 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 3 Dec 2020 19:17:35 +0000
Subject: [PATCH 017/359] Template update for nf-core/tools version 1.12.1

---
 .github/CONTRIBUTING.md                |  79 +++++++++++++++++++++++--
 .github/ISSUE_TEMPLATE/bug_report.md   |  14 +++++
 .github/PULL_REQUEST_TEMPLATE.md       |  14 +++--
 .github/markdownlint.yml               |   9 ++-
 Dockerfile                             |   2 +-
 README.md                              |  23 ++++++-
 assets/nf-core-rnafusion_logo.png      | Bin 12597 -> 109343 bytes
 conf/igenomes.config                   |  12 ++--
 docs/images/nf-core-rnafusion_logo.png | Bin 21334 -> 109343 bytes
 main.nf                                |   2 +-
 10 files changed, 137 insertions(+), 18 deletions(-)

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 7f74910f..53bc9a6b 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -18,8 +18,9 @@ If you'd like to write some code for nf-core/rnafusion, the standard workflow is
 1. Check that there isn't already an issue about your idea in the [nf-core/rnafusion issues](https://github.com/nf-core/rnafusion/issues) to avoid duplicating work
     * If there isn't one already, please create one so that others know you're working on this
 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rnafusion repository](https://github.com/nf-core/rnafusion) to your GitHub account
-3. Make the necessary changes / additions within your forked repository
-4. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged
+3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
+4. Use `nf-core schema build .` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
+5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged
 
 If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/).
 
@@ -30,14 +31,14 @@ Typically, pull-requests are only fully reviewed when these tests are passing, t
 
 There are typically two types of tests that run:
 
-### Lint Tests
+### Lint tests
 
 `nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to.
 To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint <pipeline-directory>` command.
 
 If any failures or warnings are encountered, please follow the listed URL for more documentation.
 
-### Pipeline Tests
+### Pipeline tests
 
 Each `nf-core` pipeline should be set up with a minimal set of test-data.
 `GitHub Actions` then runs the pipeline on this data to ensure that it exits successfully.
@@ -55,3 +56,73 @@ These tests are run both with the latest available version of `Nextflow` and als
 ## Getting help
 
 For further information/help, please consult the [nf-core/rnafusion documentation](https://nf-co.re/rnafusion/usage) and don't hesitate to get in touch on the nf-core Slack [#rnafusion](https://nfcore.slack.com/channels/rnafusion) channel ([join our Slack here](https://nf-co.re/join/slack)).
+
+## Pipeline contribution conventions
+
+To make the nf-core/rnafusion code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written.
+
+### Adding a new step
+
+If you wish to contribute a new step, please use the following coding standards:
+
+1. Define the corresponding input channel into your new process from the expected previous process channel
+2. Write the process block (see below).
+3. Define the output channel if needed (see below).
+4. Add any new flags/options to `nextflow.config` with a default (see below).
+5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`)
+6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter).
+7. Add sanity checks for all relevant parameters.
+8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`.
+9. Do local tests that the new code works properly and as expected.
+10. Add a new test command in `.github/workflow/ci.yaml`.
+11. If applicable add a [MultiQC](https://https://multiqc.info/) module.
+12. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, name clean up, General Statistics Table column order, and module figures are in the right order.
+13. Optional: Add any descriptions of MultiQC report sections and output files to `docs/output.md`.
+
+### Default values
+
+Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope.
+
+Once there, use `nf-core schema build .` to add to `nextflow_schema.json`.
+
+### Default processes resource requirements
+
+Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/%7B%7Bcookiecutter.name_noslash%7D%7D/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
+
+The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block.
+
+### Naming schemes
+
+Please use the following naming schemes, to make it easy to understand what is going where.
+
+* initial process channel: `ch_output_from_<process>`
+* intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
+
+### Nextflow version bumping
+
+If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]`
+
+### Software version reporting
+
+If you add a new tool to the pipeline, please ensure you add the information of the tool to the `get_software_version` process.
+
+Add to the script block of the process, something like the following:
+
+```bash
+<YOUR_TOOL> --version &> v_<YOUR_TOOL>.txt 2>&1 || true
+```
+
+or
+
+```bash
+<YOUR_TOOL> --help | head -n 1 &> v_<YOUR_TOOL>.txt 2>&1 || true
+```
+
+You then need to edit the script `bin/scrape_software_versions.py` to:
+
+1. Add a Python regex for your tool's `--version` output (as in stored in the `v_<YOUR_TOOL>.txt` file), to ensure the version is reported as a `v` and the version number e.g. `v2.1.1`
+2. Add a HTML entry to the `OrderedDict` for formatting in MultiQC.
+
+### Images and figures
+
+For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines).
diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md
index 371eaa38..4bfc81fd 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.md
+++ b/.github/ISSUE_TEMPLATE/bug_report.md
@@ -13,6 +13,13 @@ Thanks for telling us about a problem with the pipeline.
 Please delete this text and anything that's not relevant from the template below:
 -->
 
+## Check Documentation
+
+I have checked the following places for your error:
+
+- [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
+- [ ] [nf-core/rnafusion pipeline documentation](https://nf-co.re/nf-core/rnafusion/usage)
+
 ## Description of the bug
 
 <!-- A clear and concise description of what the bug is. -->
@@ -28,6 +35,13 @@ Steps to reproduce the behaviour:
 
 <!-- A clear and concise description of what you expected to happen. -->
 
+## Log files
+
+Have you provided the following extra information/files:
+
+- [ ] The command used to run the pipeline
+- [ ] The `.nextflow.log` file <!-- this is a hidden file in the directory where you launched the pipeline -->
+
 ## System
 
 - Hardware: <!-- [e.g. HPC, Desktop, Cloud...] -->
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 6e32aa01..0708ccb5 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -13,8 +13,14 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
 
 ## PR checklist
 
-- [ ] This comment contains a description of changes (with reason)
-- [ ] `CHANGELOG.md` is updated
+- [ ] This comment contains a description of changes (with reason).
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
-- [ ] Documentation in `docs` is updated
-- [ ] If necessary, also make a PR on the [nf-core/rnafusion branch on the nf-core/test-datasets repo](https://github.com/nf-core/test-datasets/pull/new/nf-core/rnafusion)
+ - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py`
+ - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+ - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
+- [ ] Make sure your code lints (`nf-core lint .`).
+- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
+- [ ] Usage Documentation in `docs/usage.md` is updated.
+- [ ] Output Documentation in `docs/output.md` is updated.
+- [ ] `CHANGELOG.md` is updated.
+- [ ] `README.md` is updated (including new tool citations and authors/contributors).
diff --git a/.github/markdownlint.yml b/.github/markdownlint.yml
index 96b12a70..8d7eb53b 100644
--- a/.github/markdownlint.yml
+++ b/.github/markdownlint.yml
@@ -1,5 +1,12 @@
 # Markdownlint configuration file
-default: true,
+default: true
 line-length: false
 no-duplicate-header:
     siblings_only: true
+no-inline-html:
+    allowed_elements:
+        - img
+        - p
+        - kbd
+        - details
+        - summary
diff --git a/Dockerfile b/Dockerfile
index 1138a469..766d425e 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.12
+FROM nfcore/base:1.12.1
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 
diff --git a/README.md b/README.md
index c0399601..b9437ee9 100644
--- a/README.md
+++ b/README.md
@@ -12,6 +12,9 @@
 
 ## Introduction
 
+<!-- TODO nf-core: Write a 1-2 sentence summary of what data the pipeline is for and what it does -->
+**nf-core/rnafusion** is a bioinformatics best-practise analysis pipeline for
+
 The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible.
 
 ## Quick Start
@@ -38,6 +41,15 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 
 See [usage docs](https://nf-co.re/rnafusion/usage) for all of the available options when running the pipeline.
 
+## Pipeline Summary
+
+By default, the pipeline currently performs the following:
+
+<!-- TODO nf-core: Fill in short bullet-pointed list of default steps of pipeline -->
+
+* Sequencing quality control (`FastQC`)
+* Overall pipeline run summaries (`MultiQC`)
+
 ## Documentation
 
 The nf-core/rnafusion pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/rnafusion/usage) and [output](https://nf-co.re/rnafusion/output).
@@ -48,13 +60,18 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline: [usa
 
 nf-core/rnafusion was originally written by Martin Proks.
 
+We thank the following people for their extensive assistance in the development
+of this pipeline:
+
+<!-- TODO nf-core: If applicable, make list of people who have also contributed -->
+
 ## Contributions and Support
 
 If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).
 
 For further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).
 
-## Citation
+## Citations
 
 <!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi. -->
 <!-- If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
@@ -67,3 +84,7 @@ You can cite the `nf-core` publication as follows:
 >
 > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).
 > ReadCube: [Full Access Link](https://rdcu.be/b1GjZ)
+
+In addition, references of tools and data used in this pipeline are as follows:
+
+<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
diff --git a/assets/nf-core-rnafusion_logo.png b/assets/nf-core-rnafusion_logo.png
index ea7ca150bb5310db9aefcda00a23cdd304e6aee9..3b70bc4aec66a46634c4952837724d10a6597399 100644
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diff --git a/conf/igenomes.config b/conf/igenomes.config
index caeafceb..31b7ee61 100644
--- a/conf/igenomes.config
+++ b/conf/igenomes.config
@@ -21,7 +21,7 @@ params {
       readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
       mito_name   = "MT"
       macs_gsize  = "2.7e9"
-      blacklist   = "${baseDir}/assets/blacklists/GRCh37-blacklist.bed"
+      blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
     }
     'GRCh38' {
       fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
@@ -33,7 +33,7 @@ params {
       bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
       mito_name   = "chrM"
       macs_gsize  = "2.7e9"
-      blacklist   = "${baseDir}/assets/blacklists/hg38-blacklist.bed"
+      blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
     }
     'GRCm38' {
       fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
@@ -46,7 +46,7 @@ params {
       readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
       mito_name   = "MT"
       macs_gsize  = "1.87e9"
-      blacklist   = "${baseDir}/assets/blacklists/GRCm38-blacklist.bed"
+      blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
     }
     'TAIR10' {
       fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
@@ -270,7 +270,7 @@ params {
       bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
       mito_name   = "chrM"
       macs_gsize  = "2.7e9"
-      blacklist   = "${baseDir}/assets/blacklists/hg38-blacklist.bed"
+      blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
     }
     'hg19' {
       fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
@@ -283,7 +283,7 @@ params {
       readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
       mito_name   = "chrM"
       macs_gsize  = "2.7e9"
-      blacklist   = "${baseDir}/assets/blacklists/hg19-blacklist.bed"
+      blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
     }
     'mm10' {
       fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
@@ -296,7 +296,7 @@ params {
       readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
       mito_name   = "chrM"
       macs_gsize  = "1.87e9"
-      blacklist   = "${baseDir}/assets/blacklists/mm10-blacklist.bed"
+      blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
     }
     'bosTau8' {
       fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
diff --git a/docs/images/nf-core-rnafusion_logo.png b/docs/images/nf-core-rnafusion_logo.png
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diff --git a/main.nf b/main.nf
index 15b16621..29222833 100644
--- a/main.nf
+++ b/main.nf
@@ -336,7 +336,7 @@ workflow.onComplete {
     def email_html = html_template.toString()
 
     // Render the sendmail template
-    def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ]
+    def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ]
     def sf = new File("$projectDir/assets/sendmail_template.txt")
     def sendmail_template = engine.createTemplate(sf).make(smail_fields)
     def sendmail_html = sendmail_template.toString()

From 9ca97479201ba7578d278fa61925aaadff89a763 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Fri, 22 Jan 2021 23:23:43 +0100
Subject: [PATCH 018/359] chore: fix TEMPLATE sync

---
 assets/nf-core-rnafusion_social_preview.png | Bin 0 -> 47745 bytes
 assets/nf-core-rnafusion_social_preview.svg | 446 ++++++++++++++++++++
 conf/genomes.config                         |  33 ++
 containers/arriba/Dockerfile                |  14 +
 containers/arriba/environment.yml           |  14 +
 containers/ericscript/Dockerfile            |  17 +
 containers/ericscript/environment.yml       |   8 +
 containers/fusioncatcher/Dockerfile         |  14 +
 containers/fusioncatcher/environment.yml    |   7 +
 containers/pizzly/Dockerfile                |  14 +
 containers/pizzly/environment.yml           |   9 +
 containers/squid/Dockerfile                 |  19 +
 containers/squid/environment.yml            |  11 +
 docs/images/BTB_logo.png                    | Bin 0 -> 13624 bytes
 docs/images/BTB_logo.svg                    | 184 ++++++++
 docs/images/NGI_logo.png                    | Bin 0 -> 20547 bytes
 docs/images/NGI_logo.svg                    | 333 +++++++++++++++
 docs/images/SDU_logo.png                    | Bin 0 -> 11894 bytes
 docs/images/SciLifeLab_logo.png             | Bin 0 -> 8387 bytes
 docs/images/SciLifeLab_logo.svg             |  99 +++++
 docs/images/nf-core-rnafusion_logo.png      | Bin 109343 -> 21334 bytes
 docs/images/rnafusion_logo.png              | Bin 0 -> 15692 bytes
 docs/images/rnafusion_logo.svg              | 208 +++++++++
 docs/images/summary_graph_1.png             | Bin 0 -> 95932 bytes
 docs/images/summary_graph_2.png             | Bin 0 -> 66019 bytes
 docs/images/summary_graph_3.png             | Bin 0 -> 83535 bytes
 docs/output.md                              | 238 ++++++++++-
 docs/usage.md                               | 413 +++++++++++++++++-
 download-references.nf                      | 260 ++++++++++++
 environment.yml                             |  12 +-
 nextflow.config                             |  57 ++-
 scripts/build.sh                            |  51 +++
 32 files changed, 2444 insertions(+), 17 deletions(-)
 create mode 100644 assets/nf-core-rnafusion_social_preview.png
 create mode 100644 assets/nf-core-rnafusion_social_preview.svg
 create mode 100644 conf/genomes.config
 create mode 100644 containers/arriba/Dockerfile
 create mode 100644 containers/arriba/environment.yml
 create mode 100644 containers/ericscript/Dockerfile
 create mode 100644 containers/ericscript/environment.yml
 create mode 100644 containers/fusioncatcher/Dockerfile
 create mode 100644 containers/fusioncatcher/environment.yml
 create mode 100644 containers/pizzly/Dockerfile
 create mode 100644 containers/pizzly/environment.yml
 create mode 100644 containers/squid/Dockerfile
 create mode 100644 containers/squid/environment.yml
 create mode 100644 docs/images/BTB_logo.png
 create mode 100644 docs/images/BTB_logo.svg
 create mode 100644 docs/images/NGI_logo.png
 create mode 100644 docs/images/NGI_logo.svg
 create mode 100644 docs/images/SDU_logo.png
 create mode 100644 docs/images/SciLifeLab_logo.png
 create mode 100644 docs/images/SciLifeLab_logo.svg
 create mode 100644 docs/images/rnafusion_logo.png
 create mode 100644 docs/images/rnafusion_logo.svg
 create mode 100644 docs/images/summary_graph_1.png
 create mode 100644 docs/images/summary_graph_2.png
 create mode 100644 docs/images/summary_graph_3.png
 create mode 100644 download-references.nf
 create mode 100755 scripts/build.sh

diff --git a/assets/nf-core-rnafusion_social_preview.png b/assets/nf-core-rnafusion_social_preview.png
new file mode 100644
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diff --git a/conf/genomes.config b/conf/genomes.config
new file mode 100644
index 00000000..934041b2
--- /dev/null
+++ b/conf/genomes.config
@@ -0,0 +1,33 @@
+/*
+ * -------------------------------------------------
+ *  Nextflow config file for reference genome
+ * -------------------------------------------------
+ * Defines reference genomes, without using iGenome paths
+ * Can be used by any config that customizes the base
+ * path using $params.genomes_base / --genomes_base
+ */
+
+params {
+    genomes {
+        'GRCh38' {
+            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh38_r${params.reference_release}.all.fa"
+            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh38_r${params.reference_release}.gtf"
+            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh38_r${params.reference_release}.cdna.all.fa.gz"
+            databases           = "${params.genomes_base}/databases"
+            arriba_ref          = "${params.genomes_base}/arriba"
+            ericscript_ref      = "${params.genomes_base}/ericscript/ericscript_db_homosapiens_ensembl84"
+            fusioncatcher_ref   = "${params.genomes_base}/fusioncatcher/human_v98"
+            star_fusion_ref     = "${params.genomes_base}/star-fusion/ctat_genome_lib_build_dir"
+        }
+        'GRCh37' {
+            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.all.fa"
+            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.gtf"
+            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.cdna.all.fa.gz"
+            databases           = "${params.genomes_base}/databases"
+            arriba_ref          = "${params.genomes_base}/arriba"
+            ericscript_ref      = "${params.genomes_base}/ericscript/ericscript_db_homosapiens_ensembl73"
+            fusioncatcher_ref   = "${params.genomes_base}/fusioncatcher/README.txt"
+            star_fusion_ref     = "${params.genomes_base}/star-fusion/ctat_genome_lib_build_dir"
+        }
+    }
+}
diff --git a/containers/arriba/Dockerfile b/containers/arriba/Dockerfile
new file mode 100644
index 00000000..f6a94950
--- /dev/null
+++ b/containers/arriba/Dockerfile
@@ -0,0 +1,14 @@
+FROM nfcore/base:1.9
+
+LABEL authors="Martin Proks" \
+    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
+
+# Install the conda environment
+COPY environment.yml /
+RUN conda env create -f /environment.yml && conda clean -a
+
+# Add conda installation dir to PATH (instead of doing 'conda activate')
+ENV PATH /opt/conda/envs/nf-core-rnafusion-arriba_1.2.0/bin:$PATH
+
+# Dump the details of the installed packages to a file for posterity
+RUN conda env export --name nf-core-rnafusion-arriba_1.2.0 > nf-core-rnafusion-arriba_1.2.0.yml
\ No newline at end of file
diff --git a/containers/arriba/environment.yml b/containers/arriba/environment.yml
new file mode 100644
index 00000000..393b2e11
--- /dev/null
+++ b/containers/arriba/environment.yml
@@ -0,0 +1,14 @@
+name: nf-core-rnafusion-arriba_1.2.0
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::arriba=1.2.0
+  - bioconda::bioconductor-genomicalignments
+  - bioconda::bioconductor-genomicranges
+  - bioconda::samtools=1.9
+  - bioconda::star=2.7.1a
+  - conda-forge::openssl=1.0
+  - conda-forge::r-circlize
+  - conda-forge::readline=6.2
\ No newline at end of file
diff --git a/containers/ericscript/Dockerfile b/containers/ericscript/Dockerfile
new file mode 100644
index 00000000..d418d1c7
--- /dev/null
+++ b/containers/ericscript/Dockerfile
@@ -0,0 +1,17 @@
+FROM nfcore/base:1.9
+
+LABEL authors="Martin Proks" \
+    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
+
+# Install the conda environment
+COPY environment.yml /
+RUN conda env create -f /environment.yml && conda clean -a
+
+# Add conda installation dir to PATH (instead of doing 'conda activate')
+ENV PATH /opt/conda/envs/nf-core-rnafusion-ericscript_0.5.5/bin:$PATH
+
+# Ignore database check (https://github.com/nf-core/rnafusion/issues/119)
+RUN echo 1 > /opt/conda/envs/nf-core-rnafusion-ericscript_0.5.5/share/ericscript-0.5.5-4/lib/data/_resources/.flag.dbexists
+
+# Dump the details of the installed packages to a file for posterity
+RUN conda env export --name nf-core-rnafusion-ericscript_0.5.5 > nf-core-rnafusion-ericscript_0.5.5.yml
\ No newline at end of file
diff --git a/containers/ericscript/environment.yml b/containers/ericscript/environment.yml
new file mode 100644
index 00000000..3edc48ce
--- /dev/null
+++ b/containers/ericscript/environment.yml
@@ -0,0 +1,8 @@
+name: nf-core-rnafusion-ericscript_0.5.5
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::ericscript=0.5.5
+  - conda-forge::ncurses=6.1
\ No newline at end of file
diff --git a/containers/fusioncatcher/Dockerfile b/containers/fusioncatcher/Dockerfile
new file mode 100644
index 00000000..203bb32a
--- /dev/null
+++ b/containers/fusioncatcher/Dockerfile
@@ -0,0 +1,14 @@
+FROM nfcore/base:1.7
+
+LABEL authors="Martin Proks" \
+    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
+
+# Install the conda environment
+COPY environment.yml /
+RUN conda env create -f /environment.yml && conda clean -a
+
+# Add conda installation dir to PATH (instead of doing 'conda activate')
+ENV PATH /opt/conda/envs/nf-core-rnafusion-fusioncatcher_1.20/bin:$PATH
+
+# Dump the details of the installed packages to a file for posterity
+RUN conda env export --name nf-core-rnafusion-fusioncatcher_1.20 > nf-core-rnafusion-fusioncatcher_1.20.yml
\ No newline at end of file
diff --git a/containers/fusioncatcher/environment.yml b/containers/fusioncatcher/environment.yml
new file mode 100644
index 00000000..1d6a105d
--- /dev/null
+++ b/containers/fusioncatcher/environment.yml
@@ -0,0 +1,7 @@
+name: nf-core-rnafusion-fusioncatcher_1.20
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::fusioncatcher=1.20
diff --git a/containers/pizzly/Dockerfile b/containers/pizzly/Dockerfile
new file mode 100644
index 00000000..2817069f
--- /dev/null
+++ b/containers/pizzly/Dockerfile
@@ -0,0 +1,14 @@
+FROM nfcore/base:1.9
+
+LABEL authors="Martin Proks" \
+    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
+
+# Install the conda environment
+COPY environment.yml /
+RUN conda env create -f /environment.yml && conda clean -a
+
+# Add conda installation dir to PATH (instead of doing 'conda activate')
+ENV PATH /opt/conda/envs/nf-core-rnafusion-pizzly_0.37.3/bin:$PATH
+
+# Dump the details of the installed packages to a file for posterity
+RUN conda env export --name nf-core-rnafusion-pizzly_0.37.3 > nf-core-rnafusion-pizzly_0.37.3.yml
\ No newline at end of file
diff --git a/containers/pizzly/environment.yml b/containers/pizzly/environment.yml
new file mode 100644
index 00000000..79974871
--- /dev/null
+++ b/containers/pizzly/environment.yml
@@ -0,0 +1,9 @@
+name: nf-core-rnafusion-pizzly_0.37.3
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::kallisto=0.44.0
+  - bioconda::pizzly=0.37.3
+  - conda-forge::pigz=2.3.4
diff --git a/containers/squid/Dockerfile b/containers/squid/Dockerfile
new file mode 100644
index 00000000..5dbcaf4c
--- /dev/null
+++ b/containers/squid/Dockerfile
@@ -0,0 +1,19 @@
+FROM nfcore/base:1.9
+
+LABEL authors="Martin Proks" \
+    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
+
+# Install the conda environment
+COPY environment.yml /
+RUN conda env create -f /environment.yml && conda clean -a
+
+# Add conda installation dir to PATH (instead of doing 'conda activate')
+ENV PATH /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin:$PATH
+
+RUN cd /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin \
+    && wget https://raw.githubusercontent.com/Kingsford-Group/squid/f45c9025d41cffd982ecbbdd52844e5a4f074de9/utils/AnnotateSQUIDOutput.py \
+    && chmod +x /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin/AnnotateSQUIDOutput.py \
+    && ln -s /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin/python3 /bin/python
+
+# Dump the details of the installed packages to a file for posterity
+RUN conda env export --name nf-core-rnafusion-squid_1.5-star2.7.1a > nf-core-rnafusion-squid_1.5-star2.7.1a.yml
\ No newline at end of file
diff --git a/containers/squid/environment.yml b/containers/squid/environment.yml
new file mode 100644
index 00000000..7385b163
--- /dev/null
+++ b/containers/squid/environment.yml
@@ -0,0 +1,11 @@
+name: nf-core-rnafusion-squid_1.5-star2.7.1a
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::samtools=1.9
+  - bioconda::squid=1.5
+  - bioconda::star=2.7.1a
+  - conda-forge::numpy=1.18.1
+  - conda-forge::python=3.7.6
diff --git a/docs/images/BTB_logo.png b/docs/images/BTB_logo.png
new file mode 100644
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diff --git a/docs/output.md b/docs/output.md
index e28adb3e..1ac968a7 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -10,16 +10,76 @@ This document describes the output produced by the pipeline. Most of the plots a
 
 The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory.
 
-<!-- TODO nf-core: Write this documentation describing your workflow's output -->
-
-## Pipeline overview
+## Pipeline overview <!-- omit in toc -->
 
 The pipeline is built using [Nextflow](https://www.nextflow.io/)
 and processes data using the following steps:
 
-* [FastQC](#fastqc) - Read quality control
-* [MultiQC](#multiqc) - Aggregate report describing results from the whole pipeline
-* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
+- [nf-core/rnafusion: Output](#nf-corernafusion-output)
+  - [:warning: Please read this documentation on the nf-core website: https://nf-co.re/rnafusion/output](#warning-please-read-this-documentation-on-the-nf-core-website-httpsnf-corernafusionoutput)
+  - [Introduction](#introduction)
+  - [Arriba](#arriba)
+  - [EricScript](#ericscript)
+  - [FastQC](#fastqc)
+  - [Fusioncatcher](#fusioncatcher)
+  - [Fusion Inspector](#fusion-inspector)
+  - [fusion-report](#fusion-report)
+    - [Tool detection](#tool-detection)
+    - [Found in database](#found-in-database)
+    - [Tool detection distribution](#tool-detection-distribution)
+  - [MultiQC](#multiqc)
+  - [Pipeline information](#pipeline-information)
+  - [Pizzly](#pizzly)
+  - [Squid](#squid)
+  - [Star-Fusion](#star-fusion)
+
+## Arriba
+
+**Output directory: `results/tools/Arriba`**
+
+- `fusions.tsv`
+  - contains fusions which pass all of Arriba's filters. It should be highly enriched for true predictions. The predictions are listed from highest to lowest confidence.
+- `fusions.discarded.tsv`
+  - contains all events that Arriba classified as an artifact or that are also observed in healthy tissue. This file may be useful, if one suspects that an event should be present, but was erroneously discarded by Arriba.
+- `<sample>.pdf`
+  - contains fusion visualization when opted for `--arriba_vis`
+
+## EricScript
+
+**Output directory: `results/tools/Ericscript/tmp`**
+
+- `fusions.results.filtered.tsv`
+  - contains all the predicted gene fusions
+
+|      Column      |                                        Description                                        |
+| ---------------- | ----------------------------------------------------------------------------------------- |
+| GeneName1        | official gene name of 5' gene.                                                            |
+| GeneName2        | official gene name of 3' gene.                                                            |
+| chr1             | chromosome of 5' gene.                                                                    |
+| Breakpoint1      | predicted breakpoint on 5' gene.                                                          |
+| strand1          | strand (-/+) of 5' gene.                                                                  |
+| chr2             | chromosome of 3' gene.                                                                    |
+| Breakpoint2      | predicted breakpoint on 3' gene.                                                          |
+| strand2          | strand (-/+) of 3' gene.                                                                  |
+| EnsemblGene1     | Ensembl gene ID of 5' gene.                                                               |
+| EnsemblGene2     | Ensembl gene ID of 3' gene.                                                               |
+| crossingreads    | number of paired end discordant reads.                                                    |
+| spanningreads    | number of paired end reads spanning the junction.                                         |
+| mean.insertsize  | mean of insert sizes of crossing + spanning reads.                                        |
+| homology         | if filled, all the homologies between the fusion junction and Ensembl genes.              |
+| fusiontype       | intra-chromosomal, inter-chromosomal, read-through or CIS.                                |
+| InfoGene1        | gene information about 5' gene.                                                           |
+| InfoGene2        | gene information about 3' gene.                                                           |
+| JunctionSequence | predicted junction fusion sequence.                                                       |
+| GeneExpr1        | Read count based estimation of the expression level of 5' gene.                           |
+| GeneExpr2        | Read count based estimation of the expression level of 3' gene.                           |
+| GeneExpr_fused   | Read count based estimation of the expression level of the predicted chimeric transcript. |
+| ES               | Edge score.                                                                               |
+| GJS              | Genuine Junction score.                                                                   |
+| US               | Uniformity score.                                                                         |
+| EricScore        | EricScore score (adaboost classifier).                                                    |
+
+For more info check the [documentation](https://sites.google.com/site/bioericscript/getting-started).
 
 ## FastQC
 
@@ -29,12 +89,107 @@ For further reading and documentation see the [FastQC help pages](http://www.bio
 
 **Output files:**
 
+> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads.
+> They may contain adapter sequence and potentially regions with low quality.
+
 * `fastqc/`
   * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files.
 * `fastqc/zips/`
   * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
-> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
+## Fusioncatcher
+
+**Output directory: `results/tools/Fusioncatcher`**
+
+- `final-list_candidate-fusion-genes.txt`
+  - contains all the predicted gene fusions
+
+| Column | Description |
+| ------ | ----------- |
+| **Gene\_1\_symbol(5end\_fusion\_partner)** | Gene symbol of the 5' end fusion partner |
+| **Gene\_2\_symbol\_2(3end\_fusion\_partner)** | Gene symbol of the 3' end fusion partner |
+| **Gene\_1\_id(5end\_fusion\_partner)** | Ensembl gene id of the 5' end fusion partner |
+| **Gene\_2\_id(3end\_fusion\_partner)** | Ensembl gene id of the 3' end fusion partner |
+| **Exon\_1\_id(5end\_fusion\_partner)** | Ensembl exon id of the 5' end fusion exon-exon junction |
+| **Exon\_2\_id(3end\_fusion\_partner)** | Ensembl exon id of the 3' end fusion exon-exon junction |
+| **Fusion\_point\_for\_gene\_1(5end\_fusion\_partner)** | Chromosomal position of the 5' end of fusion junction (chromosome:position:strand); 1-based coordinate |
+| **Fusion\_point\_for\_gene\_2(3end\_fusion\_partner)** | Chromosomal position of the 3' end of fusion junction (chromosome:position:strand); 1-based coordinate |
+| **Spanning\_pairs** | Count of pairs of reads supporting the fusion (**including** also the multimapping reads) |
+| **Spanning\_unique\_reads** | Count of unique reads (i.e. unique mapping positions) mapping on the fusion junction. Shortly, here are counted all the reads which map on fusion junction minus the PCR duplicated reads. |
+| **Longest\_anchor\_found** | Longest anchor (hangover) found among the unique reads mapping on the fusion junction |
+| **Fusion\_finding\_method** | Aligning method used for mapping the reads and finding the fusion genes. Here are the methods used: |
+| | (i) **BOWTIE** = only Bowtie aligner is used for mapping the reads on the genome and exon-exon fusion junctions |
+| | (ii) **BOWTIE+BLAT** = Bowtie aligner is used for mapping reads on the genome and BLAT is used for mapping reads for finding the fusion junction |
+| | (iii) **BOWTIE+STAR** = Bowtie aligner is used for mapping reads on the genome and STAR is used for mapping reads for finding the fusion junction |
+| | (iv) **BOWTIE+BOWTIE2** = Bowtie aligner is used for mapping reads on the genome and Bowtie2 is used for mapping reads for finding the fusion junction |
+| **Fusion\_sequence** | The inferred fusion junction (the asterisk sign marks the junction point) |
+| **Fusion\_description** | Type of the fusion gene (see the Table 2) |
+| **Counts\_of\_common\_mapping\_reads** | Count of reads mapping simultaneously on both genes which form the fusion gene. This is an indication how similar are the DNA/RNA sequences of the genes forming the fusion gene (i.e. what is their homology because highly homologous genes tend to appear show as candidate fusion genes). In case of completely different sequences of the genes involved in forming a fusion gene then here it is expected to have the value zero. |
+| **Predicted\_effect** | Predicted effect of the candidate fusion gene using the annotation from Ensembl database. This is shown in format **effect\_gene\_1**/**effect\_gene\_2**, where the possible values for effect\_gene\_1 or effect\_gene\_2 are: **intergenic**, **intronic**, **exonic(no-known-CDS)**, **UTR**, **CDS(not-reliable-start-or-end)**, **CDS(truncated)**, or **CDS(complete)**. In case that the fusion junction for both genes is within their CDS (coding sequence) then only the values **in-frame** or **out-of-frame** will be shown. |
+| **Predicted\_fused\_transcripts** | All possible known fused transcripts in format ENSEMBL-TRANSCRIPT-1:POSITION-1/ENSEMBLE-TRANSCRIPT-B:POSITION-2, where are fused the sequence 1:POSITION-1 of transcript ENSEMBL-TRANSCRIPT-1 with sequence POSITION-2:END of transcript ENSEMBL-TRANSCRIPT-2 |
+| **Predicted\_fused\_proteins** | Predicted amino acid sequences of all possible fused proteins (separated by ";").  |
+
+For more info check the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md#62---output-data-output-data).
+
+## Fusion Inspector
+
+**Output directory: `results/tools/FusionInspector`**
+
+- `finspector.fa`
+  - the candidate fusion-gene contigs (if you copy things elsewhere, make sure to also copy the index file: `finspector.fa.fai`)
+- `finspector.bed`
+  - the reference gene structure annotations for fusion partners
+- `finspector.junction_reads.bam`
+  - alignments of the breakpoint-junction supporting reads.
+- `finspector.spanning_reads.bam`
+  - alignments of the breakpoint-spanning paired-end reads.
+
+To visualize fusion genes in [IGV tool](https://software.broadinstitute.org/software/igv/igvtools) first create a genome `Menu->Genomes->Create .genome File`, choose name and description, then choose the following files:
+
+- `finspector.fa`
+  - make sure the index file finspector.fa.fai is in the same folder
+- `finspector.gtf`
+  - use this for 'Genes'
+- `cytoBand.txt`
+  - use this for 'optional Cytoband'
+
+Add the bam files by choosing `File->Load from File` and make sure to select your generated mini genome in the upper-left corner.
+For more info and help check [wiki page](https://github.com/FusionInspector/FusionInspector/wiki).
+
+## fusion-report
+
+**Output directory: `results/Report-<READS_BASE_NAME>`**
+
+- `fusions.json`
+  - contains all main information about found fusions (fusion name, score, explanation of the score calculation, cherry picked output from fusion tools)
+- `index.html`
+  - main dashboard containing the list of all detected fusions
+- `*.html`
+  - each fusion gets a custom page with fetched data from the local database
+- `fusions_list_filtered.txt`
+  - filtered list of found fusions (uses tool cutoff as filter, by default: 2, can be adjusted by adding `-t <num>` when running the tool)
+- `fusions_list.txt`
+  - unfiltered list of found fusions
+- `<READS_BASE_NAME>_fusion-report.tar.gz`
+  - tarball of the above files to allow easier import to local machine(s) for visualisation through browser
+
+### Tool detection
+
+Graphs displaying ratio of fusion genes caught by different tools. The last part *all tools* is an intersection of all tools.
+
+![Tool detection](images/summary_graph_1.png)
+
+### Found in database
+
+Displays how many fusions were found in a downloaded databases of the summary report.
+
+![Known/unknown fusions](images/summary_graph_2.png)
+
+### Tool detection distribution
+
+For each fusion a sum of detected tools is calculated. This counts are then visualized in the graph below.
+
+![Known/unknown fusions](images/summary_graph_3.png)
 
 ## MultiQC
 
@@ -52,7 +207,6 @@ For more information about how to use MultiQC reports, see [https://multiqc.info
   * `multiqc_plots/`: directory containing static images from the report in various formats.
 
 ## Pipeline information
-
 [Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
 
 **Output files:**
@@ -61,3 +215,71 @@ For more information about how to use MultiQC reports, see [https://multiqc.info
   * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
   * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`.
   * Documentation for interpretation of results in HTML format: `results_description.html`.
+
+## Pizzly
+
+**Output directory: `results/tools/Pizzly`**
+
+- `pizzly_fusions.json`
+  - contains all the predicted gene fusions
+
+|  Column | Description |
+| ------- | ----------- |
+| geneA | `id`: reference id and `name`: gene name |
+| geneB | Describes reference id and gene name |
+| paircount | Number of paired count |
+| splitcount | Number of split count |
+| transcripts | List of all transcripts `fasta_record`, `transcriptA`, `transcriptB`, `support`, `reads` |
+| readpairs | List of read pairs containing (`type`, `read1`, `read2`) |
+
+For more info check the [documentation](https://github.com/pmelsted/pizzly#output).
+
+## Squid
+
+**Output directory: `results/tools/Squid`**
+
+- `fusions_annotated.txt`
+  - contains all the predicted gene fusions
+
+|  Column | Description |
+| ------- | ----------- |
+| chr1 | chromosome name of the first breakpoint.
+| start1 | starting position of the segment of the first breakpoint, or the predicted breakpoint position if strand1 is "-" |
+| end1 | ending position of the segment of the first breakpoint, or the predicted breakpoint position if strand1 is "+" |
+| chr2 | chromosome name of the second breakpoint |
+| start2 | starting position of the segment of the second breakpoint, or the predicted breakpoint position if strand2 is "-" |
+| end2 | ending position of the segment of the second breakpoint, or the predicted breakpoint position if strand2 is "+" |
+| name | TSV is not named yet, this column shows with dot.
+| score | number of reads supporting this TSV (without weighted by Discordant edge ratio multiplier) |
+| strand1 | strand of the first segment in TSV.
+| strand2 | strand of the second segment in TSV.
+| num_concordantfrag_bp1 | number of concordant paired-end reads covering the first breakpoint. For a concordant paired-end read, it includes two ends and a inserted region in between, if any of the 3 regions covers the breakpoint, the read is counted in this number |
+| num_concordantfrag_bp2 | number of concordant paired-end reads covering the second breakpoint. The count is defined in the same way as num_concordantfrag_bp1 |
+
+For more info check the [documentation](https://github.com/Kingsford-Group/squid#output-specification).
+
+## Star-Fusion
+
+**Output directory: `results/tools/StarFusion`**
+
+- `star-fusion.fusion_predictions.tsv`
+  - contains all the predicted gene fusions
+
+|  Column | Description |
+| ------- | ----------- |
+| JunctionReadCount | Indicates the number of RNA-Seq fragments containing a read that aligns as a split read at the site of the putative fusion junction. |
+| SpanningFragCount | Indicates the number of RNA-Seq fragments that encompass the fusion junction such that one read of the pair aligns to a different gene than the other paired-end read of that fragment. |
+| SpliceType | Indicates whether the proposed breakpoint occurs at reference exon junctions as provided by the reference transcript structure annotations (ex. gencode).
+| LeftGene
+| LeftBreakpoint
+| RightGene
+| RightBreakpoint
+| LargeAnchorSupport | column indicates whether there are split reads that provide 'long' (set to length of 25 bases) alignments on both sides of the putative breakpoint. |
+| FFPM | fusion fragments per million total reads; **Default:** *0.1 (meaning at least 1 fusion-supporting rna-seq fragment per 10M total reads)*; **TL;DR:** can be adjusted by changing `--min_FFPM`
+| LeftBreakDinuc |  |
+| LeftBreakEntropy | Represents Shannon entropy |
+| RightBreakDinuc |
+| RightBreakEntropy | Represents Shannon entropy |
+| annots | Annotation generated by [FusionAnnotar](https://github.com/FusionAnnotator/FusionAnnotator/wiki) |
+
+For more info check the [documentation](https://github.com/STAR-Fusion/STAR-Fusion/wiki#Outputs).
diff --git a/docs/usage.md b/docs/usage.md
index dd7292d5..30b5011e 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -1,4 +1,4 @@
-# nf-core/rnafusion: Usage
+# nf-core/rnafusion: Usage <!-- omit in toc -->
 
 ## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/rnafusion/usage](https://nf-co.re/rnafusion/usage)
 
@@ -6,14 +6,175 @@
 
 ## Introduction
 
-<!-- TODO nf-core: Add documentation about anything specific to running your pipeline. For general topics, please point to (and add to) the main nf-core website. -->
+- [:warning: Please read this documentation on the nf-core website: https://nf-co.re/rnafusion/usage](#warning-please-read-this-documentation-on-the-nf-core-website-httpsnf-corernafusionusage)
+- [Introduction](#introduction)
+- [Download references](#download-references)
+  - [Download all references](#download-all-references)
+  - [Download specific references](#download-specific-references)
+  - [Download and build CTAT](#download-and-build-ctat)
+  - [Download GRCh37 references](#download-grch37-references)
+  - [Tool reference requirements](#tool-reference-requirements)
+- [Running the pipeline](#running-the-pipeline)
+  - [Updating the pipeline](#updating-the-pipeline)
+  - [Reproducibility](#reproducibility)
+- [Core Nextflow arguments](#core-nextflow-arguments)
+  - [`-profile`](#-profile)
+  - [`-resume`](#-resume)
+  - [`-c`](#-c)
+    - [Custom resource requests](#custom-resource-requests)
+  - [Running in the background](#running-in-the-background)
+    - [Nextflow memory requirements](#nextflow-memory-requirements)
+- [Pipeline specific arguments](#pipeline-specific-arguments)
+  - [--input](#--input)
+  - [--single_end](#--single_end)
+  - [Tool flags](#tool-flags)
+  - [--arriba](#--arriba)
+  - [--ericscript](#--ericscript)
+  - [--fusioncatcher](#--fusioncatcher)
+  - [--fusion_report](#--fusion_report)
+  - [--pizzly](#--pizzly)
+  - [--squid](#--squid)
+  - [--star_fusion](#--star_fusion)
+- [Visualization flags](#visualization-flags)
+  - [--arriba_vis](#--arriba_vis)
+  - [--fusion_inspector](#--fusion_inspector)
+- [Reference genomes](#reference-genomes)
+  - [--arriba_ref](#--arriba_ref)
+  - [--databases](#--databases)
+  - [--ericscript_ref](#--ericscript_ref)
+  - [--fasta](#--fasta)
+  - [--fusioncatcher_ref](#--fusioncatcher_ref)
+  - [--genome](#--genome)
+  - [--gtf](#--gtf)
+  - [--reference_release](#--reference_release)
+  - [--star_index](#--star_index)
+  - [--star_fusion_ref](#--star_fusion_ref)
+  - [--transcript](#--transcript)
+- [Other command line parameters](#other-command-line-parameters)
+  - [--debug](#--debug)
+  - [--read_length](#--read_length)
+  - [--outdir](#--outdir)
+  - [--email](#--email)
+  - [--email_on_fail](#--email_on_fail)
+  - [--max_multiqc_email_size](#--max_multiqc_email_size)
+  - [-name](#-name)
+  - [--custom_config_version](#--custom_config_version)
+  - [--custom_config_base](#--custom_config_base)
+- [Job resources](#job-resources)
+  - [Automatic resubmission](#automatic-resubmission)
+
+## Download references
+
+The rnafusion pipeline needs references for the fusion detection tools, so downloading these is a prerequisite.
+
+Downloading references manually is a tedious long process.
+To make the pipeline easier to work with, we provide a script to download all necessary references for fusion detection tools.
+
+> **TL;DR:** Make sure to download the correct references for your need!
+
+### Download all references
+
+```bash
+# Replace <COSMIC_USER> and <COSMIC_PASSWD> with yout credentials from COSMIC
+nextflow run nf-core/rnafusion/download-references.nf \
+  --download_all \
+  --outdir <PATH> \
+  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+```
+
+### Download specific references
+
+```bash
+# Example of downloading base references
+
+nextflow run nf-core/rnafusion/download-references.nf \
+--base \
+--outdir <PATH>
+
+# Example of downloading references for specific tool
+
+nextflow run nf-core/rnafusion/download-references.nf \
+--arriba \
+--outdir <PATH>
+
+nextflow run nf-core/rnafusion/download-references.nf \
+--star_fusion \
+--outdir <PATH>
+
+nextflow run nf-core/rnafusion/download-references.nf \
+--fusioncatcher \
+--outdir <PATH>
+
+nextflow run nf-core/rnafusion/download-references.nf \
+--ericscript \
+--outdir <PATH>
+
+nextflow run nf-core/rnafusion/download-references.nf \
+--fusion_report \
+--outdir <PATH>
+  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+```
+
+### Download and build CTAT
+
+```bash
+nextflow run nf-core/rnafusion/build-ctat.nf \
+  --genome GRCh38 \
+  --outdir <PATH> \
+  --fasta <PATH>/<FASTA \
+  --gtf <PATH>/<GTF>
+```
+
+### Download GRCh37 references
+
+```bash
+# GRCh38 genome assembly is used by default.
+# To use the previous assembly specify it using the --genome flag
+nextflow run nf-core/rnafusion/download-references.nf \
+  --genome GRCh37 \
+  --download_all \
+  --outdir <PATH> \
+  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+
+# Please note that using the above example command downloads NCBI-based references for STAR-Fusion.
+# To use Ensembl-based references run the following command with the same <PATH> as used above
+
+nextflow run nf-core/rnafusion/build-ctat.nf \
+  --genome GRCh37 \
+  --outdir <PATH> \
+  --fasta <PATH>/<FASTA> \
+  --gtf <PATH>/<GTF>
+```
+
+### Tool reference requirements
+
+| Tool             |        FASTA       |         GTF        |     STAR-index     |     Genome     |       Other       |
+| ---------------- | :----------------: | :----------------: | :----------------: | :------------: | :----------------: |
+| Arriba           | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 | `custom_reference` |
+| EricScript       |         :x:        |         :x:        |         :x:        | GRCh37, GRCh38 | `custom_reference` |
+| FusionCatcher    |         :x:        |         :x:        |         :x:        |     GRCh38     | `custom_reference` |
+| Fusion-Inspector | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |  `ctat_genome_lib` |
+| fusion-report    |         :x:        |         :x:        |         :x:        | GRCh37, GRCh38 |     `databases`    |
+| Pizzly           |         :x:        | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |       `cDNA`       |
+| Squid            |         :x:        | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |          -         |
+| Star-Fusion      | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |  `ctat_genome_lib` |
 
 ## Running the pipeline
 
-The typical command for running the pipeline is as follows:
+The typical command for running the pipeline is as follows.
 
 ```bash
-nextflow run nf-core/rnafusion --input '*_R{1,2}.fastq.gz' -profile docker
+nextflow run nf-core/rnafusion \
+  -profile docker \
+  --input "*_R{1,2}.fastq.gz" \
+  --arriba \
+  --star_fusion \
+  --fusioncatcher \
+  --ericscript \
+  --pizzly \
+  --squid \
+  --arriba_vis \
+  --fusion_inspector
 ```
 
 This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
@@ -126,3 +287,247 @@ We recommend adding the following line to your environment to limit this (typica
 ```bash
 NXF_OPTS='-Xms1g -Xmx4g'
 ```
+
+## Pipeline specific arguments
+
+### --input
+
+Use this to specify the location of your input FastQ files. For example:
+
+```bash
+--input 'path/to/data/sample_*_{1,2}.fastq.gz'
+```
+
+Please note the following requirements:
+
+1. The path must be enclosed in quotes
+2. The path must have at least one `*` wildcard character
+3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.
+
+If left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`
+
+### --single_end
+
+By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:
+
+```bash
+--single_end --input '*.fastq'
+```
+
+### Tool flags
+
+### --arriba
+
+If enabled, executes `Arriba` tool.
+
+- `--arriba_opt`
+  - Specify additional parameters. For more information, please refer to the [documentation](http://arriba.readthedocs.io/en/latest/quickstart/) of the tool.
+
+### --ericscript
+
+If enabled, executes `Ericscript` tool.
+
+- `--ericscript_opt`
+  - Specify additional parameters. For more information, please refer to the [documentation](https://sites.google.com/site/bioericscript/home) of the tool.
+
+### --fusioncatcher
+
+If enabled, executes `Fusioncatcher` tool.
+
+> N.B. that Fusioncatcher is not available when using the `GRCh37` genome assembly.
+
+- `--fusioncatcher_opt`
+  - Specify additional parameters. For more information, please refer to the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md) of the tool.
+
+### --fusion_report
+
+If enabled, download databases for `fusion-report`.
+
+- `fusion_report_opt`
+  - Specify additional parameters. For more information, please refer to the [documentation](https://matq007.github.io/fusion-report/#/) of the tool.
+
+### --pizzly
+
+If enabled, executes `Pizzly` tool.
+
+- `--pizzly_k`
+  - Number of k-mers. Default `31`.
+
+### --squid
+
+If enabled, executes `Squid` tool.
+
+### --star_fusion
+
+If enabled, executes `STAR-Fusion` tool.
+
+- `--star_fusion_opt`
+  - Parameter for specifying additional parameters. For more information, please refer to the [documentation](https://github.com/STAR-Fusion/STAR-Fusion/wiki) of the tool.
+
+## Visualization flags
+
+### --arriba_vis
+
+If enabled, executes build in `Arriba` visualization tool.
+
+### --fusion_inspector
+
+If enabled, executes `Fusion-Inspector` tool.
+
+## Reference genomes
+
+### --arriba_ref
+
+```bash
+--arriba_ref '<path to Arriba reference>'
+```
+
+### --databases
+
+Required databases in order to run `fusion-report`.
+
+```bash
+--databases '<path to fusion-report databases>'
+```
+
+### --ericscript_ref
+
+Required reference in order to run `EricScript`.
+
+```bash
+--ericscript_ref '<path to EricScript reference>'
+```
+
+### --fasta
+
+If you prefer, you can specify the full path to your reference genome when you run the pipeline:
+
+```bash
+--fasta '<path to Fasta reference>'
+```
+
+### --fusioncatcher_ref
+
+Required reference in order to run `Fusioncatcher`.
+
+```bash
+--fusioncatcher_ref '<path to Fusioncatcher reference>'
+```
+
+### --genome
+
+This pipeline uses `Homo Sapiens` version `GRCh38` by default. Assembly `GRCh37` is optionally available.
+
+> N.B. that using `GRCh37` precludes use of the `Fusioncatcher` tool.
+> Also make sure to specify `--genomes_base`.
+
+```bash
+--genome 'GRCh38' --genome_base '</path/to/references>'
+```
+
+### --gtf
+
+Required annotation file.
+
+```bash
+--gtf '<path to GTF annotation>'
+```
+
+### --reference_release
+
+Ensembl version.
+
+```bash
+# ftp://ftp.ensembl.org/pub/release-97/fasta/homo_sapiens/
+--reference_release '97'
+```
+
+### --star_index
+
+If you prefer, you can specify the full path for `STAR` index when you run the pipeline.
+If not specified, the pipeline will build the index using for reads with length `100bp` (can be adjusted with parameter `--read_length`).
+
+```bash
+--star_index '<path to STAR index>'
+```
+
+### --star_fusion_ref
+
+Required reference in order to run `STAR-Fusion`.
+
+```bash
+--star_fusion_ref '<path to STAR-Fusion reference>'
+```
+
+### --transcript
+
+Required transcript file.
+
+```bash
+--transcript '<path to transcript reference>'
+```
+
+## Other command line parameters
+
+### --debug
+
+To run only a specific tool (testing freshly implemented tool) just add `--debug` parameter.
+This parameter only works on **fusion tools only**!
+
+### --read_length
+
+Length is used to build a STAR index.
+Default is `100bp` (Illumina).
+
+### --outdir
+
+The output directory where the results will be saved.
+
+### --email
+
+Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits.
+If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.
+
+### --email_on_fail
+
+This works exactly as with `--email`, except emails are only sent if the workflow is not successful.
+
+### --max_multiqc_email_size
+
+Threshold size for MultiQC report to be attached in notification email.
+If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB).
+
+### -name
+
+Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic.
+This is used in the MultiQC report (if not default) and in the summary HTML / e-mail (always).
+
+**NB:** Single hyphen (core Nextflow option)
+
+### --custom_config_version
+
+Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.
+
+```bash
+## Download and use config file with following git commid id
+--custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96
+```
+
+### --custom_config_base
+
+If you're running offline, nextflow will not be able to fetch the institutional config files
+from the internet. If you don't need them, then this is not a problem. If you do need them,
+you should download the files from the repo and tell nextflow where to find them with the
+`custom_config_base` option. For example:
+
+```bash
+NXF_OPTS='-Xms1g -Xmx4g'
+```
+
+## Job resources
+
+### Automatic resubmission
+
+Each step in the pipeline has a default set of requirements for number of CPUs, memory and time.
+For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original).
+If it still fails after three times then the pipeline is stopped.
diff --git a/download-references.nf b/download-references.nf
new file mode 100644
index 00000000..4dd34b66
--- /dev/null
+++ b/download-references.nf
@@ -0,0 +1,260 @@
+#!/usr/bin/env nextflow
+/*
+================================================================================
+                                nf-core/rnafusion
+================================================================================
+nf-core/rnafusion:
+ RNA-seq analysis pipeline for detection gene-fusions
+--------------------------------------------------------------------------------
+ @Homepage
+ https://nf-co.re/rnafusion
+--------------------------------------------------------------------------------
+ @Documentation
+ https://nf-co.re/rnafusion/docs
+--------------------------------------------------------------------------------
+ @Repository
+ https://github.com/nf-core/rnafusion
+--------------------------------------------------------------------------------
+*/
+
+def helpMessage() {
+    log.info nfcoreHeader()
+    log.info"""
+    Usage:
+
+    The typical command for downloading references is as follows:
+
+    nextflow run nf-core/rnafusion/download-references.nf -profile [PROFILE] [OPTIONS] --outdir /path/to/output
+
+    Mandatory arguments:
+      --outdir [path]               Output directory for downloading
+
+    Options:
+      --download_all [bool]         Download all references
+      --genome [str]                Genome version: GRCh37 or GRCh38 (default)
+      --reference_release [int]     Release number of Ensembl reference for FASTA and GTF
+                                    Default: 97 -> ftp://ftp.ensembl.org/pub/release-97
+      --base [bool]                 Download FASTA, GTF, cDNA
+      --arriba [bool]               Download Arriba references
+      --star_fusion [bool]          Build STAR-Fusion references from FASTA ANF GTF
+      --fusioncatcher [bool]        Download Fusioncatcher references
+      --ericscript [bool]           Download Ericscript references
+      --fusion_report [bool]        Download databases for fusion-report
+      --cosmic_usr [str]            [Required with fusion_report] COSMIC username
+      --cosmic_passwd [str]         [Required with fusion_report] COSMIC password
+    """.stripIndent()
+}
+
+/*
+ * SET UP CONFIGURATION VARIABLES
+ */
+
+// Show help emssage
+if (params.help) exit 0, helpMessage()
+if (!params.outdir) exit 1, "Output directory not specified!"
+
+running_tools = []
+if (params.base || params.download_all) running_tools.add("Reference v${params.reference_release}")
+if (params.arriba || params.download_all) running_tools.add("Arriba")
+if (params.star_fusion || params.download_all) running_tools.add("STAR-Fusion")
+if (params.fusioncatcher || params.download_all) running_tools.add("Fusioncatcher")
+if (params.ericscript || params.download_all) running_tools.add("Ericscript")
+if (params.fusion_report || params.download_all) {
+    running_tools.add('fusion-report')
+    if (!params.cosmic_usr || !params.cosmic_passwd) exit 1, "Database credentials are required parameter!"
+}
+
+// Header log info
+log.info nfcoreHeader()
+def summary = [:]
+summary['Pipeline Name']  = 'nf-core/rnafusion/download-references.nf'
+summary['Pipeline Version'] = workflow.manifest.version
+summary['References']       = running_tools.size() == 0 ? 'None' : running_tools.join(", ")
+if(workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container"
+summary['Max Resources']    = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job"
+summary['Output dir']   = params.outdir
+summary['User']         = workflow.userName
+log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n")
+log.info "\033[2m----------------------------------------------------\033[0m"
+
+// Check the hostnames against configured profiles
+checkHostname()
+
+/*
+================================================================================
+                                  DOWNLOAD
+================================================================================
+*/
+
+process download_base {
+    publishDir "${params.outdir}/", mode: 'copy'
+
+    when:
+    params.base || params.download_all
+
+    output:
+    file "Homo_sapiens.${params.genome}_r${params.reference_release}.all.fa" into fasta
+    file "Homo_sapiens.${params.genome}_r${params.reference_release}.gtf" into gtf
+    file "Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz" into transcript
+
+    script:
+    def base_url = params.genome == "GRCh37" ? "ftp://ftp.ensembl.org/pub/grch37" : "ftp://ftp.ensembl.org/pub/"
+    """
+    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
+    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
+    gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}_r${params.reference_release}.all.fa
+    wget ${base_url}/release-${params.reference_release}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${params.reference_release}.chr.gtf.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
+    gunzip Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
+    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz
+    """
+}
+
+process download_arriba {
+    publishDir "${params.outdir}/arriba", mode: 'copy'
+
+    when:
+    params.arriba || params.download_all
+
+    output:
+    file '*'
+
+    script:
+    """
+    wget -N https://github.com/suhrig/arriba/releases/download/v1.2.0/arriba_v1.2.0.tar.gz -O arriba_v1.2.0.tar.gz
+    tar -xvzf arriba_v1.2.0.tar.gz && mv arriba_v1.2.0/database/* . && gunzip *.gz && rm -rf arriba_*
+    """
+}
+
+process download_star_fusion {
+    publishDir "${params.outdir}/star-fusion", mode: 'copy'
+
+    when:
+    params.star_fusion || params.download_all
+
+    output:
+    file '*'
+
+    script:
+    """
+    if [[ ${params.genome} == "GRCh37" ]]; then
+      wget -O GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.9/GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz
+      tar -xf GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz --strip-components=5
+      rm GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz
+    else
+      aws s3 --no-sign-request --region ${params.awsregion} cp s3://ngi-igenomes/Homo_sapiens/Ensembl/GRCh38/Genome/CTAT/ctat_star_fusion_1_8_1.tar.gz .
+      tar -xf ctat_star_fusion_1_8_1.tar.gz --strip-components=5
+      rm ctat_star_fusion_1_8_1.tar.gz
+    fi
+    """
+}
+
+process download_fusioncatcher {
+    publishDir "${params.outdir}/fusioncatcher", mode: 'copy'
+
+    when:
+    params.fusioncatcher || params.download_all
+
+    output:
+    file '*'
+
+    script:
+    """
+    if [[ ${params.genome} == "GRCh37" ]]; then
+      echo "FusionCatcher unavailable for GRCh37" > README.txt
+    else
+      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.aa
+      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ab
+      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ac
+      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ad
+      cat human_v98.tar.gz.* | tar xz
+      rm human_v98.tar*
+    fi
+    """
+}
+
+process download_ericscript {
+    publishDir "${params.outdir}/ericscript", mode: 'copy'
+
+    when:
+    params.ericscript || params.download_all
+
+    output:
+    file '*'
+
+    script:
+    def base_url = params.genome == "GRCh37" ? "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIibDRIb0RFdHFlQmM" : "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIiUGt1SnFMZFg4TlE"
+    """
+    if [[ ${params.genome} == "GRCh37" ]]; then
+      gdown ${base_url} ericscript_db_homosapiens_ensembl73.tar.bz2
+    else
+      gdown ${base_url} ericscript_db_homosapiens_ensembl84.tar.bz2
+    fi
+    tar jxf ericscript_db_homosapiens_ensembl*.tar.bz2 && rm ericscript_db_homosapiens_ensembl*.tar.bz2
+    """
+}
+
+process download_databases {
+    publishDir "${params.outdir}/databases", mode: 'copy'
+
+    when:
+    params.fusion_report || params.download_all
+
+    output:
+    file '*'
+
+    script:
+    """
+    fusion_report download --cosmic_usr "${params.cosmic_usr}" --cosmic_passwd "${params.cosmic_passwd}" .
+    """
+}
+
+/*
+ * Completion
+ */
+workflow.onComplete {
+    log.info "[nf-core/rnafusion/download-references.nf] Pipeline Complete"
+}
+
+def nfcoreHeader() {
+    // Log colors ANSI codes
+    c_black = params.monochrome_logs ? '' : "\033[0;30m";
+    c_blue = params.monochrome_logs ? '' : "\033[0;34m";
+    c_cyan = params.monochrome_logs ? '' : "\033[0;36m";
+    c_dim = params.monochrome_logs ? '' : "\033[2m";
+    c_green = params.monochrome_logs ? '' : "\033[0;32m";
+    c_purple = params.monochrome_logs ? '' : "\033[0;35m";
+    c_reset = params.monochrome_logs ? '' : "\033[0m";
+    c_white = params.monochrome_logs ? '' : "\033[0;37m";
+    c_yellow = params.monochrome_logs ? '' : "\033[0;33m";
+
+    return """    -${c_dim}--------------------------------------------------${c_reset}-
+                                            ${c_green},--.${c_black}/${c_green},-.${c_reset}
+    ${c_blue}        ___     __   __   __   ___     ${c_green}/,-._.--~\'${c_reset}
+    ${c_blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${c_yellow}}  {${c_reset}
+    ${c_blue}  | \\| |       \\__, \\__/ |  \\ |___     ${c_green}\\`-._,-`-,${c_reset}
+                                            ${c_green}`._,._,\'${c_reset}
+    ${c_purple}  nf-core/rnafusion v${workflow.manifest.version}${c_reset}
+    -${c_dim}--------------------------------------------------${c_reset}-
+    """.stripIndent()
+}
+
+def checkHostname() {
+    def c_reset = params.monochrome_logs ? '' : "\033[0m"
+    def c_white = params.monochrome_logs ? '' : "\033[0;37m"
+    def c_red = params.monochrome_logs ? '' : "\033[1;91m"
+    def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m"
+    if (params.hostnames) {
+        def hostname = "hostname".execute().text.trim()
+        params.hostnames.each { prof, hnames ->
+            hnames.each { hname ->
+                if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
+                    log.error "====================================================\n" +
+                            "  ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" +
+                            "  but your machine hostname is ${c_white}'$hostname'${c_reset}\n" +
+                            "  ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" +
+                            "============================================================"
+                }
+            }
+        }
+    }
+}
diff --git a/environment.yml b/environment.yml
index 162bdacd..4f754e10 100644
--- a/environment.yml
+++ b/environment.yml
@@ -10,6 +10,16 @@ dependencies:
   - conda-forge::markdown=3.1.1
   - conda-forge::pymdown-extensions=6.0
   - conda-forge::pygments=2.5.2
-  # TODO nf-core: Add required software dependencies here
+  # Necessary tools
+  - conda-forge::gdown=3.12.0
   - bioconda::fastqc=0.11.8
   - bioconda::multiqc=1.7
+  # Custom packages
+  - bioconda::star=2.7.1a # has to be for star index
+  - conda-forge::r-data.table=1.12.8
+  - conda-forge::r-gplots=3.0.1.2
+  - bioconda::bioconductor-edger=3.28.0
+  - bioconda::fusion-report=2.1.3
+  # Star-Fusion
+  - conda-forge::awscli=1.18.39
+  - conda-forge::tar=1.32
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index d23ddf6f..c8b8a9ae 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -7,12 +7,59 @@
 
 // Global default params, used in configs
 params {
+  genome = 'GRCh38'
+  genomes_base = false
+  reference_release = '97'
 
-  // Workflow flags
-  // TODO nf-core: Specify your pipeline's command line flags
-  genome = false
+  // Options: Building STAR-star_index
+  star_index = false
+  read_length = 100
+
+  // Fusion tools
+  arriba = false
+  star_fusion = false
+  fusioncatcher = false
+  fusion_inspector = false
+  ericscript = false
+  pizzly = false
+  squid = false
+
+  // Options: Arriba
+  arriba_opt = false
+  arriba_vis = false
+
+  // Options: STAR-Fusion
+  star_fusion_opt = false
+
+  // Options: FusionCatcher
+  fusioncatcher_opt = false
+  
+  // Options: Pizzly
+  pizzly_k = 31
+
+  // Options: Fusion-Inspector
+  fusion_inspector_opt = false
+
+  // Options: fusion-report
+  fusion_report_opt = false
+
+  // Defaults
   input = "data/*{1,2}.fastq.gz"
   single_end = false
+  clusterOptions = false
+  awsqueue = false
+  awsregion = 'eu-west-1'
+  input_paths = null
+  debug = false
+
+  // Options: download-references.nf
+  base = false
+  download_all = false
+  fusion_report = false
+  cosmic_usr = false
+  cosmic_passwd = false
+
+  // Shared default variables across different scripts
   outdir = './results'
   publish_dir_mode = 'copy'
 
@@ -39,7 +86,6 @@ params {
   max_memory = 128.GB
   max_cpus = 16
   max_time = 240.h
-
 }
 
 // Container slug. Stable releases should specify release tag!
@@ -56,6 +102,9 @@ try {
   System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
 }
 
+// Load genomes.config
+includeConfig 'conf/genomes.config'
+
 profiles {
   conda { process.conda = "$projectDir/environment.yml" }
   debug { process.beforeScript = 'echo $HOSTNAME' }
diff --git a/scripts/build.sh b/scripts/build.sh
new file mode 100755
index 00000000..5c439c43
--- /dev/null
+++ b/scripts/build.sh
@@ -0,0 +1,51 @@
+#!/bin/bash
+
+PREFIX="nfcore/rnafusion"
+
+create_container() {
+    TOOL_PATH=$1
+    VERSION="$(cat $TOOL_PATH/environment.yml | grep "name:" | cut -d":" -f2 | cut -d "_" -f2)"
+    TOOL_NAME=`basename $TOOL_PATH`
+    CONTAINER_NAME="${PREFIX}:${TOOL_NAME}_${VERSION}"
+    echo "Building [$CONTAINER_NAME]"
+    docker build $TOOL_PATH -t $CONTAINER_NAME
+    docker push $CONTAINER_NAME
+}
+
+if [ $# -eq 0 ]; then
+    echo "No tool name specified!"
+    echo "Run scripts/build.sh -h for help"
+    exit 1
+fi
+
+if [ $1 == "-h" ]; then
+    echo "Utility for building docker containers from tools/"
+    echo "Usage: scripts/build.sh [options]"
+    echo 
+    echo "Options:"
+    echo "  all             build all tools including main image"
+    echo "  <tool name>     builds specific tool"
+    echo "                  Example: sh scripts/build.sh ericscript"
+    exit 0
+fi
+
+if [ $1 == "all" ]; then
+    for TOOL in containers/*/; do
+        create_container `pwd`/$TOOL ${TOOL%?}
+    done
+    # Build main container
+    VERSION="$(cat nextflow.config | grep -m1 "container" | cut -d":" -f2 | cut -d "'" -f1)"
+    CONTAINER_NAME=$PREFIX:$VERSION
+    echo "Building [$CONTAINER_NAME]"
+    docker build . -t $CONTAINER_NAME
+    docker push $CONTAINER_NAME
+else
+    TOOL=$1
+    TOOL_PATH="$(pwd)/containers/$TOOL"
+    if [ ! -d $TOOL_PATH ]; then
+        echo "The tool doesn't exist"
+        exit 1
+    else
+        create_container $TOOL_PATH
+    fi
+fi

From 90d0ae7a12488b9e5d7bae87d4ab740c85bd7cca Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Fri, 22 Jan 2021 23:34:23 +0100
Subject: [PATCH 019/359] docs: updated output

---
 docs/output.md | 85 +++++++++++++++++++++++++-------------------------
 1 file changed, 43 insertions(+), 42 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 1ac968a7..8474db08 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -37,19 +37,19 @@ and processes data using the following steps:
 
 **Output directory: `results/tools/Arriba`**
 
-- `fusions.tsv`
-  - contains fusions which pass all of Arriba's filters. It should be highly enriched for true predictions. The predictions are listed from highest to lowest confidence.
-- `fusions.discarded.tsv`
-  - contains all events that Arriba classified as an artifact or that are also observed in healthy tissue. This file may be useful, if one suspects that an event should be present, but was erroneously discarded by Arriba.
-- `<sample>.pdf`
-  - contains fusion visualization when opted for `--arriba_vis`
+* `fusions.tsv`
+  * contains fusions which pass all of Arriba's filters. It should be highly enriched for true predictions. The predictions are listed from highest to lowest confidence.
+* `fusions.discarded.tsv`
+  * contains all events that Arriba classified as an artifact or that are also observed in healthy tissue. This file may be useful, if one suspects that an event should be present, but was erroneously discarded by Arriba.
+* `<sample>.pdf`
+  * contains fusion visualization when opted for `--arriba_vis`
 
 ## EricScript
 
 **Output directory: `results/tools/Ericscript/tmp`**
 
-- `fusions.results.filtered.tsv`
-  - contains all the predicted gene fusions
+* `fusions.results.filtered.tsv`
+  * contains all the predicted gene fusions
 
 |      Column      |                                        Description                                        |
 | ---------------- | ----------------------------------------------------------------------------------------- |
@@ -101,8 +101,8 @@ For further reading and documentation see the [FastQC help pages](http://www.bio
 
 **Output directory: `results/tools/Fusioncatcher`**
 
-- `final-list_candidate-fusion-genes.txt`
-  - contains all the predicted gene fusions
+* `final-list_candidate-fusion-genes.txt`
+  * contains all the predicted gene fusions
 
 | Column | Description |
 | ------ | ----------- |
@@ -135,23 +135,23 @@ For more info check the [documentation](https://github.com/ndaniel/fusioncatcher
 
 **Output directory: `results/tools/FusionInspector`**
 
-- `finspector.fa`
-  - the candidate fusion-gene contigs (if you copy things elsewhere, make sure to also copy the index file: `finspector.fa.fai`)
-- `finspector.bed`
-  - the reference gene structure annotations for fusion partners
-- `finspector.junction_reads.bam`
-  - alignments of the breakpoint-junction supporting reads.
-- `finspector.spanning_reads.bam`
-  - alignments of the breakpoint-spanning paired-end reads.
+* `finspector.fa`
+  * the candidate fusion-gene contigs (if you copy things elsewhere, make sure to also copy the index file: `finspector.fa.fai`)
+* `finspector.bed`
+  * the reference gene structure annotations for fusion partners
+* `finspector.junction_reads.bam`
+  * alignments of the breakpoint-junction supporting reads.
+* `finspector.spanning_reads.bam`
+  * alignments of the breakpoint-spanning paired-end reads.
 
 To visualize fusion genes in [IGV tool](https://software.broadinstitute.org/software/igv/igvtools) first create a genome `Menu->Genomes->Create .genome File`, choose name and description, then choose the following files:
 
-- `finspector.fa`
-  - make sure the index file finspector.fa.fai is in the same folder
-- `finspector.gtf`
-  - use this for 'Genes'
-- `cytoBand.txt`
-  - use this for 'optional Cytoband'
+* `finspector.fa`
+  * make sure the index file finspector.fa.fai is in the same folder
+* `finspector.gtf`
+  * use this for 'Genes'
+* `cytoBand.txt`
+  * use this for 'optional Cytoband'
 
 Add the bam files by choosing `File->Load from File` and make sure to select your generated mini genome in the upper-left corner.
 For more info and help check [wiki page](https://github.com/FusionInspector/FusionInspector/wiki).
@@ -160,18 +160,18 @@ For more info and help check [wiki page](https://github.com/FusionInspector/Fusi
 
 **Output directory: `results/Report-<READS_BASE_NAME>`**
 
-- `fusions.json`
-  - contains all main information about found fusions (fusion name, score, explanation of the score calculation, cherry picked output from fusion tools)
-- `index.html`
-  - main dashboard containing the list of all detected fusions
-- `*.html`
-  - each fusion gets a custom page with fetched data from the local database
-- `fusions_list_filtered.txt`
-  - filtered list of found fusions (uses tool cutoff as filter, by default: 2, can be adjusted by adding `-t <num>` when running the tool)
-- `fusions_list.txt`
-  - unfiltered list of found fusions
-- `<READS_BASE_NAME>_fusion-report.tar.gz`
-  - tarball of the above files to allow easier import to local machine(s) for visualisation through browser
+* `fusions.json`
+  * contains all main information about found fusions (fusion name, score, explanation of the score calculation, cherry picked output from fusion tools)
+* `index.html`
+  * main dashboard containing the list of all detected fusions
+* `*.html`
+  * each fusion gets a custom page with fetched data from the local database
+* `fusions_list_filtered.txt`
+  * filtered list of found fusions (uses tool cutoff as filter, by default: 2, can be adjusted by adding `-t <num>` when running the tool)
+* `fusions_list.txt`
+  * unfiltered list of found fusions
+* `<READS_BASE_NAME>_fusion-report.tar.gz`
+  * tarball of the above files to allow easier import to local machine(s) for visualisation through browser
 
 ### Tool detection
 
@@ -207,6 +207,7 @@ For more information about how to use MultiQC reports, see [https://multiqc.info
   * `multiqc_plots/`: directory containing static images from the report in various formats.
 
 ## Pipeline information
+
 [Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
 
 **Output files:**
@@ -220,8 +221,8 @@ For more information about how to use MultiQC reports, see [https://multiqc.info
 
 **Output directory: `results/tools/Pizzly`**
 
-- `pizzly_fusions.json`
-  - contains all the predicted gene fusions
+* `pizzly_fusions.json`
+  * contains all the predicted gene fusions
 
 |  Column | Description |
 | ------- | ----------- |
@@ -238,8 +239,8 @@ For more info check the [documentation](https://github.com/pmelsted/pizzly#outpu
 
 **Output directory: `results/tools/Squid`**
 
-- `fusions_annotated.txt`
-  - contains all the predicted gene fusions
+* `fusions_annotated.txt`
+  * contains all the predicted gene fusions
 
 |  Column | Description |
 | ------- | ----------- |
@@ -262,8 +263,8 @@ For more info check the [documentation](https://github.com/Kingsford-Group/squid
 
 **Output directory: `results/tools/StarFusion`**
 
-- `star-fusion.fusion_predictions.tsv`
-  - contains all the predicted gene fusions
+* `star-fusion.fusion_predictions.tsv`
+  * contains all the predicted gene fusions
 
 |  Column | Description |
 | ------- | ----------- |

From 98acd41642308509ee7a7d0bd0ba053e3560d73e Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Fri, 22 Jan 2021 23:39:16 +0100
Subject: [PATCH 020/359] docs: fix markdown for output.md

---
 docs/output.md | 108 ++++++++++++++++++++++++-------------------------
 1 file changed, 54 insertions(+), 54 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 8474db08..2d523981 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -37,19 +37,19 @@ and processes data using the following steps:
 
 **Output directory: `results/tools/Arriba`**
 
-* `fusions.tsv`
-  * contains fusions which pass all of Arriba's filters. It should be highly enriched for true predictions. The predictions are listed from highest to lowest confidence.
-* `fusions.discarded.tsv`
-  * contains all events that Arriba classified as an artifact or that are also observed in healthy tissue. This file may be useful, if one suspects that an event should be present, but was erroneously discarded by Arriba.
-* `<sample>.pdf`
-  * contains fusion visualization when opted for `--arriba_vis`
+- `fusions.tsv`
+  - contains fusions which pass all of Arriba's filters. It should be highly enriched for true predictions. The predictions are listed from highest to lowest confidence.
+- `fusions.discarded.tsv`
+  - contains all events that Arriba classified as an artifact or that are also observed in healthy tissue. This file may be useful, if one suspects that an event should be present, but was erroneously discarded by Arriba.
+- `<sample>.pdf`
+  - contains fusion visualization when opted for `--arriba_vis`
 
 ## EricScript
 
 **Output directory: `results/tools/Ericscript/tmp`**
 
-* `fusions.results.filtered.tsv`
-  * contains all the predicted gene fusions
+- `fusions.results.filtered.tsv`
+  - contains all the predicted gene fusions
 
 |      Column      |                                        Description                                        |
 | ---------------- | ----------------------------------------------------------------------------------------- |
@@ -92,17 +92,17 @@ For further reading and documentation see the [FastQC help pages](http://www.bio
 > **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads.
 > They may contain adapter sequence and potentially regions with low quality.
 
-* `fastqc/`
-  * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files.
-* `fastqc/zips/`
-  * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
+- `fastqc/`
+  - `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files.
+- `fastqc/zips/`
+  - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
 ## Fusioncatcher
 
 **Output directory: `results/tools/Fusioncatcher`**
 
-* `final-list_candidate-fusion-genes.txt`
-  * contains all the predicted gene fusions
+- `final-list_candidate-fusion-genes.txt`
+  - contains all the predicted gene fusions
 
 | Column | Description |
 | ------ | ----------- |
@@ -135,23 +135,23 @@ For more info check the [documentation](https://github.com/ndaniel/fusioncatcher
 
 **Output directory: `results/tools/FusionInspector`**
 
-* `finspector.fa`
-  * the candidate fusion-gene contigs (if you copy things elsewhere, make sure to also copy the index file: `finspector.fa.fai`)
-* `finspector.bed`
-  * the reference gene structure annotations for fusion partners
-* `finspector.junction_reads.bam`
-  * alignments of the breakpoint-junction supporting reads.
-* `finspector.spanning_reads.bam`
-  * alignments of the breakpoint-spanning paired-end reads.
+- `finspector.fa`
+  - the candidate fusion-gene contigs (if you copy things elsewhere, make sure to also copy the index file: `finspector.fa.fai`)
+- `finspector.bed`
+  - the reference gene structure annotations for fusion partners
+- `finspector.junction_reads.bam`
+  - alignments of the breakpoint-junction supporting reads.
+- `finspector.spanning_reads.bam`
+  - alignments of the breakpoint-spanning paired-end reads.
 
 To visualize fusion genes in [IGV tool](https://software.broadinstitute.org/software/igv/igvtools) first create a genome `Menu->Genomes->Create .genome File`, choose name and description, then choose the following files:
 
-* `finspector.fa`
-  * make sure the index file finspector.fa.fai is in the same folder
-* `finspector.gtf`
-  * use this for 'Genes'
-* `cytoBand.txt`
-  * use this for 'optional Cytoband'
+- `finspector.fa`
+  - make sure the index file finspector.fa.fai is in the same folder
+- `finspector.gtf`
+  - use this for 'Genes'
+- `cytoBand.txt`
+  - use this for 'optional Cytoband'
 
 Add the bam files by choosing `File->Load from File` and make sure to select your generated mini genome in the upper-left corner.
 For more info and help check [wiki page](https://github.com/FusionInspector/FusionInspector/wiki).
@@ -160,18 +160,18 @@ For more info and help check [wiki page](https://github.com/FusionInspector/Fusi
 
 **Output directory: `results/Report-<READS_BASE_NAME>`**
 
-* `fusions.json`
-  * contains all main information about found fusions (fusion name, score, explanation of the score calculation, cherry picked output from fusion tools)
-* `index.html`
-  * main dashboard containing the list of all detected fusions
-* `*.html`
-  * each fusion gets a custom page with fetched data from the local database
-* `fusions_list_filtered.txt`
-  * filtered list of found fusions (uses tool cutoff as filter, by default: 2, can be adjusted by adding `-t <num>` when running the tool)
-* `fusions_list.txt`
-  * unfiltered list of found fusions
-* `<READS_BASE_NAME>_fusion-report.tar.gz`
-  * tarball of the above files to allow easier import to local machine(s) for visualisation through browser
+- `fusions.json`
+  - contains all main information about found fusions (fusion name, score, explanation of the score calculation, cherry picked output from fusion tools)
+- `index.html`
+  - main dashboard containing the list of all detected fusions
+- `*.html`
+  - each fusion gets a custom page with fetched data from the local database
+- `fusions_list_filtered.txt`
+  - filtered list of found fusions (uses tool cutoff as filter, by default: 2, can be adjusted by adding `-t <num>` when running the tool)
+- `fusions_list.txt`
+  - unfiltered list of found fusions
+- `<READS_BASE_NAME>_fusion-report.tar.gz`
+  - tarball of the above files to allow easier import to local machine(s) for visualisation through browser
 
 ### Tool detection
 
@@ -201,10 +201,10 @@ For more information about how to use MultiQC reports, see [https://multiqc.info
 
 **Output files:**
 
-* `multiqc/`
-  * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
-  * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
-  * `multiqc_plots/`: directory containing static images from the report in various formats.
+- `multiqc/`
+  - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
+  - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
+  - `multiqc_plots/`: directory containing static images from the report in various formats.
 
 ## Pipeline information
 
@@ -212,17 +212,17 @@ For more information about how to use MultiQC reports, see [https://multiqc.info
 
 **Output files:**
 
-* `pipeline_info/`
-  * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
-  * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`.
-  * Documentation for interpretation of results in HTML format: `results_description.html`.
+- `pipeline_info/`
+  - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
+  - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`.
+  - Documentation for interpretation of results in HTML format: `results_description.html`.
 
 ## Pizzly
 
 **Output directory: `results/tools/Pizzly`**
 
-* `pizzly_fusions.json`
-  * contains all the predicted gene fusions
+- `pizzly_fusions.json`
+  - contains all the predicted gene fusions
 
 |  Column | Description |
 | ------- | ----------- |
@@ -239,8 +239,8 @@ For more info check the [documentation](https://github.com/pmelsted/pizzly#outpu
 
 **Output directory: `results/tools/Squid`**
 
-* `fusions_annotated.txt`
-  * contains all the predicted gene fusions
+- `fusions_annotated.txt`
+  - contains all the predicted gene fusions
 
 |  Column | Description |
 | ------- | ----------- |
@@ -263,8 +263,8 @@ For more info check the [documentation](https://github.com/Kingsford-Group/squid
 
 **Output directory: `results/tools/StarFusion`**
 
-* `star-fusion.fusion_predictions.tsv`
-  * contains all the predicted gene fusions
+- `star-fusion.fusion_predictions.tsv`
+  - contains all the predicted gene fusions
 
 |  Column | Description |
 | ------- | ----------- |

From b61c9e1d0fde788cb34a600fd39904d4999a0b1c Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 18 Mar 2021 12:05:23 +0000
Subject: [PATCH 021/359] Template update for nf-core/tools version 1.13

---
 .github/.dockstore.yml                    |   1 +
 .github/CONTRIBUTING.md                   |   4 +-
 .github/ISSUE_TEMPLATE/bug_report.md      |   2 +-
 .github/ISSUE_TEMPLATE/feature_request.md |   2 +-
 .github/PULL_REQUEST_TEMPLATE.md          |   6 +-
 .github/workflows/awsfulltest.yml         |  17 +-
 .github/workflows/awstest.yml             |  17 +-
 .github/workflows/branch.yml              |   7 +-
 .github/workflows/linting.yml             |   2 +-
 CODE_OF_CONDUCT.md                        | 117 ++++-
 Dockerfile                                |   6 +-
 README.md                                 |   7 +-
 assets/email_template.html                |   1 -
 assets/nf-core-rnafusion_logo.png         | Bin 109343 -> 18138 bytes
 bin/markdown_to_html.py                   |   0
 bin/scrape_software_versions.py           |   0
 conf/base.config                          |   2 +-
 conf/test.config                          |   2 +
 conf/test_full.config                     |   2 +
 docs/images/nf-core-rnafusion_logo.png    | Bin 109343 -> 35022 bytes
 docs/output.md                            |   4 -
 docs/usage.md                             |  12 +-
 lib/Headers.groovy                        |  43 ++
 lib/NfcoreSchema.groovy                   | 571 ++++++++++++++++++++++
 lib/nfcore_external_java_deps.jar         | Bin 0 -> 2291171 bytes
 main.nf                                   | 143 ++----
 nextflow.config                           |  42 +-
 nextflow_schema.json                      |  27 +-
 28 files changed, 866 insertions(+), 171 deletions(-)
 mode change 100755 => 100644 bin/markdown_to_html.py
 mode change 100755 => 100644 bin/scrape_software_versions.py
 create mode 100644 lib/Headers.groovy
 create mode 100644 lib/NfcoreSchema.groovy
 create mode 100644 lib/nfcore_external_java_deps.jar

diff --git a/.github/.dockstore.yml b/.github/.dockstore.yml
index 030138a0..191fabd2 100644
--- a/.github/.dockstore.yml
+++ b/.github/.dockstore.yml
@@ -3,3 +3,4 @@ version: 1.2
 workflows:
   - subclass: nfl
     primaryDescriptorPath: /nextflow.config
+    publish: True
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 53bc9a6b..96741701 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -69,7 +69,7 @@ If you wish to contribute a new step, please use the following coding standards:
 2. Write the process block (see below).
 3. Define the output channel if needed (see below).
 4. Add any new flags/options to `nextflow.config` with a default (see below).
-5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`)
+5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`).
 6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter).
 7. Add sanity checks for all relevant parameters.
 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`.
@@ -87,7 +87,7 @@ Once there, use `nf-core schema build .` to add to `nextflow_schema.json`.
 
 ### Default processes resource requirements
 
-Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/%7B%7Bcookiecutter.name_noslash%7D%7D/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
+Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
 
 The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block.
 
diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md
index 4bfc81fd..381c7c56 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.md
+++ b/.github/ISSUE_TEMPLATE/bug_report.md
@@ -55,7 +55,7 @@ Have you provided the following extra information/files:
 
 ## Container engine
 
-- Engine: <!-- [e.g. Conda, Docker, Singularity or Podman] -->
+- Engine: <!-- [e.g. Conda, Docker, Singularity, Podman, Shifter or Charliecloud] -->
 - version: <!-- [e.g. 1.0.0] -->
 - Image tag: <!-- [e.g. nfcore/rnafusion:1.0.0] -->
 
diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md
index 8bad5163..10da2c83 100644
--- a/.github/ISSUE_TEMPLATE/feature_request.md
+++ b/.github/ISSUE_TEMPLATE/feature_request.md
@@ -1,6 +1,6 @@
 ---
 name: Feature request
-about: Suggest an idea for the nf-core website
+about: Suggest an idea for the nf-core/rnafusion pipeline
 labels: enhancement
 ---
 
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 0708ccb5..fd43dd87 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -15,9 +15,9 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
 
 - [ ] This comment contains a description of changes (with reason).
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
- - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py`
- - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
- - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
+  - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py`
+  - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+  - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint .`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index eb4441e2..e404dd20 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -9,6 +9,16 @@ on:
     types: [completed]
   workflow_dispatch:
 
+
+env:
+  AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }}
+  AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }}
+  TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }}
+  AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }}
+  AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }}
+  AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }}
+
+
 jobs:
   run-awstest:
     name: Run AWS full tests
@@ -27,13 +37,6 @@ jobs:
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
         # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command
-        env:
-          AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }}
-          AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }}
-          TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }}
-          AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }}
-          AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }}
-          AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }}
         run: |
           aws batch submit-job \
             --region eu-west-1 \
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 9829a938..aacabe1d 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -6,6 +6,16 @@ name: nf-core AWS test
 on:
   workflow_dispatch:
 
+
+env:
+  AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }}
+  AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }}
+  TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }}
+  AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }}
+  AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }}
+  AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }}
+
+
 jobs:
   run-awstest:
     name: Run AWS tests
@@ -23,13 +33,6 @@ jobs:
         # TODO nf-core: You can customise CI pipeline run tests as required
         # For example: adding multiple test runs with different parameters
         # Remember that you can parallelise this by using strategy.matrix
-        env:
-          AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }}
-          AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }}
-          TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }}
-          AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }}
-          AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }}
-          AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }}
         run: |
           aws batch submit-job \
           --region eu-west-1 \
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 51392d1a..8a6b59d2 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -13,7 +13,7 @@ jobs:
       - name: Check PRs
         if: github.repository == 'nf-core/rnafusion'
         run: |
-          { [[ ${{github.event.pull_request.head.repo.full_name}} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
+          { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
 
 
       # If the above check failed, post a comment on the PR explaining the failure
@@ -25,11 +25,12 @@ jobs:
           message: |
             Hi @${{ github.event.pull_request.user.login }},
 
-            It looks like this pull-request is has been made against the ${{github.event.pull_request.head.repo.full_name}} `master` branch.
+            It looks like this pull-request is has been made against the ${{github.event.pull_request.base.repo.full_name }} `master` branch.
             The `master` branch on nf-core repositories should always contain code from the latest release.
-            Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.head.repo.full_name}} `dev` branch.
+            Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.base.repo.full_name }} `dev` branch.
 
             You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page.
+            Note that even after this, the test will continue to show as failing until you push a new commit.
 
             Thanks again for your contribution!
           repo-token: ${{ secrets.GITHUB_TOKEN }}
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index bef81e61..58e281df 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -69,7 +69,7 @@ jobs:
         if: ${{ always() }}
         uses: actions/upload-artifact@v2
         with:
-          name: linting-log-file
+          name: linting-logs
           path: |
             lint_log.txt
             lint_results.md
diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md
index 405fb1bf..f4fd052f 100644
--- a/CODE_OF_CONDUCT.md
+++ b/CODE_OF_CONDUCT.md
@@ -1,46 +1,111 @@
-# Contributor Covenant Code of Conduct
+# Code of Conduct at nf-core (v1.0)
 
 ## Our Pledge
 
-In the interest of fostering an open and welcoming environment, we as contributors and maintainers pledge to making participation in our project and our community a harassment-free experience for everyone, regardless of age, body size, disability, ethnicity, gender identity and expression, level of experience, nationality, personal appearance, race, religion, or sexual identity and orientation.
+In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of:
 
-## Our Standards
+- Age
+- Body size
+- Familial status
+- Gender identity and expression
+- Geographical location
+- Level of experience
+- Nationality and national origins
+- Native language
+- Physical and neurological ability
+- Race or ethnicity
+- Religion
+- Sexual identity and orientation
+- Socioeconomic status
 
-Examples of behavior that contributes to creating a positive environment include:
+Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance.
 
-* Using welcoming and inclusive language
-* Being respectful of differing viewpoints and experiences
-* Gracefully accepting constructive criticism
-* Focusing on what is best for the community
-* Showing empathy towards other community members
+## Preamble
 
-Examples of unacceptable behavior by participants include:
+> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply.
 
-* The use of sexualized language or imagery and unwelcome sexual attention or advances
-* Trolling, insulting/derogatory comments, and personal or political attacks
-* Public or private harassment
-* Publishing others' private information, such as a physical or electronic address, without explicit permission
-* Other conduct which could reasonably be considered inappropriate in a professional setting
+An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva.
+
+nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals.
+
+We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc.
+
+Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities.
+
+We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC.
+
+Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re
 
 ## Our Responsibilities
 
-Project maintainers are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behavior.
+The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour.
+
+The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful.
+
+Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC.
+
+## When are where does this Code of Conduct apply?
+
+Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference:
+
+- Communicating with an official project email address.
+- Communicating with community members within the nf-core Slack channel.
+- Participating in hackathons organised by nf-core (both online and in-person events).
+- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence.
+- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc.
+- Representing nf-core on social media. This includes both official and personal accounts.
+
+## nf-core cares 😊
+
+nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order):
+
+- Ask for consent before sharing another community member’s personal information (including photographs) on social media.
+- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity.
+- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !)
+- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.)
+- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can)
+- Focus on what is best for the team and the community. (When in doubt, ask)
+- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn.
+- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!)
+- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**)
+- Take breaks when you feel like you need them.
+- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.)
+
+## nf-core frowns on 😕
+
+The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces.
+
+- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom.
+- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online.
+- Spamming or trolling of individuals on social media.
+- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention.
+- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience.
+
+### Online Trolling
+
+The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately.
+
+All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls.
+
+## Procedures for Reporting CoC violations
 
-Project maintainers have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful.
+If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible.
 
-## Scope
+You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s).
 
-This Code of Conduct applies both within project spaces and in public spaces when an individual is representing the project or its community. Examples of representing a project or community include using an official project e-mail address, posting via an official social media account, or acting as an appointed representative at an online or offline event. Representation of a project may be further defined and clarified by project maintainers.
+Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course.
 
-## Enforcement
+All reports will be handled with utmost discretion and confidentially.
 
-Instances of abusive, harassing, or otherwise unacceptable behavior may be reported by contacting the project team on [Slack](https://nf-co.re/join/slack). The project team will review and investigate all complaints, and will respond in a way that it deems appropriate to the circumstances. The project team is obligated to maintain confidentiality with regard to the reporter of an incident. Further details of specific enforcement policies may be posted separately.
+## Attribution and Acknowledgements
 
-Project maintainers who do not follow or enforce the Code of Conduct in good faith may face temporary or permanent repercussions as determined by other members of the project's leadership.
+- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4)
+- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition)
+- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/)
+- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla)
 
-## Attribution
+## Changelog
 
-This Code of Conduct is adapted from the [Contributor Covenant][homepage], version 1.4, available at [https://www.contributor-covenant.org/version/1/4/code-of-conduct/][version]
+### v1.0 - March 12th, 2021
 
-[homepage]: https://contributor-covenant.org
-[version]: https://www.contributor-covenant.org/version/1/4/code-of-conduct/
+- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC.
diff --git a/Dockerfile b/Dockerfile
index 766d425e..b0562bed 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.12.1
+FROM nfcore/base:1.13
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 
@@ -11,7 +11,3 @@ ENV PATH /opt/conda/envs/nf-core-rnafusion-1.3.0dev/bin:$PATH
 
 # Dump the details of the installed packages to a file for posterity
 RUN conda env export --name nf-core-rnafusion-1.3.0dev > nf-core-rnafusion-1.3.0dev.yml
-
-# Instruct R processes to use these empty files instead of clashing with a local version
-RUN touch .Rprofile
-RUN touch .Renviron
diff --git a/README.md b/README.md
index b9437ee9..0ac143f5 100644
--- a/README.md
+++ b/README.md
@@ -21,12 +21,12 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 
 1. Install [`nextflow`](https://nf-co.re/usage/installation)
 
-2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) or [`Podman`](https://podman.io/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
+2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
 
 3. Download the pipeline and test it on a minimal dataset with a single command:
 
     ```bash
-    nextflow run nf-core/rnafusion -profile test,<docker/singularity/podman/conda/institute>
+    nextflow run nf-core/rnafusion -profile test,<docker/singularity/podman/shifter/charliecloud/conda/institute>
     ```
 
     > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
@@ -36,7 +36,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
     <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
 
     ```bash
-    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/conda/institute> --input '*_R{1,2}.fastq.gz' --genome GRCh37
+    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input '*_R{1,2}.fastq.gz' --genome GRCh37
     ```
 
 See [usage docs](https://nf-co.re/rnafusion/usage) for all of the available options when running the pipeline.
@@ -83,7 +83,6 @@ You can cite the `nf-core` publication as follows:
 > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.
 >
 > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).
-> ReadCube: [Full Access Link](https://rdcu.be/b1GjZ)
 
 In addition, references of tools and data used in this pipeline are as follows:
 
diff --git a/assets/email_template.html b/assets/email_template.html
index 2e85801c..efcd0eaa 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -1,6 +1,5 @@
 <html>
 <head>
-  <head>
   <meta charset="utf-8">
   <meta http-equiv="X-UA-Compatible" content="IE=edge">
   <meta name="viewport" content="width=device-width, initial-scale=1">
diff --git a/assets/nf-core-rnafusion_logo.png b/assets/nf-core-rnafusion_logo.png
index 3b70bc4aec66a46634c4952837724d10a6597399..e3b18bf3d2adae1b7f0b3ca752fe50f0fa9665cc 100644
GIT binary patch
literal 18138
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diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py
old mode 100755
new mode 100644
diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
old mode 100755
new mode 100644
diff --git a/conf/base.config b/conf/base.config
index e2a4c1ac..33eaf0a7 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -47,5 +47,5 @@ process {
   withName:get_software_versions {
     cache = false
   }
-  
+
 }
diff --git a/conf/test.config b/conf/test.config
index c84e3197..15ca6fd7 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -23,4 +23,6 @@ params {
     ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']],
     ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']]
   ]
+  // Ignore `--input` as otherwise the parameter validation will throw an error
+  schema_ignore_params = 'genomes,input_paths,input'
 }
diff --git a/conf/test_full.config b/conf/test_full.config
index 64a344d4..91c0c736 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -19,4 +19,6 @@ params {
     ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']],
     ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']]
   ]
+  // Ignore `--input` as otherwise the parameter validation will throw an error
+  schema_ignore_params = 'genomes,input_paths,input'
 }
diff --git a/docs/images/nf-core-rnafusion_logo.png b/docs/images/nf-core-rnafusion_logo.png
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diff --git a/docs/output.md b/docs/output.md
index e28adb3e..a3a11a12 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -1,9 +1,5 @@
 # nf-core/rnafusion: Output
 
-## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/rnafusion/output](https://nf-co.re/rnafusion/output)
-
-> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._
-
 ## Introduction
 
 This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline.
diff --git a/docs/usage.md b/docs/usage.md
index dd7292d5..3be033f0 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -51,7 +51,7 @@ This version number will be logged in reports when you run the pipeline, so that
 
 Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments.
 
-Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Conda) - see below.
+Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below.
 
 > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
 
@@ -71,8 +71,14 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
 * `podman`
   * A generic configuration profile to be used with [Podman](https://podman.io/)
   * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+* `shifter`
+  * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
+  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+* `charliecloud`
+  * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
+  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
 * `conda`
-  * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity or Podman.
+  * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
   * A generic configuration profile to be used with [Conda](https://conda.io/docs/)
   * Pulls most software from [Bioconda](https://bioconda.github.io/)
 * `test`
@@ -103,6 +109,8 @@ process {
 }
 ```
 
+To find the exact name of a process you wish to modify the compute resources, check the live-status of a nextflow run displayed on your terminal or check the nextflow error for a line like so: `Error executing process > 'bwa'`. In this case the name to specify in the custom config file is `bwa`.
+
 See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information.
 
 If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition above). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile.
diff --git a/lib/Headers.groovy b/lib/Headers.groovy
new file mode 100644
index 00000000..15d1d388
--- /dev/null
+++ b/lib/Headers.groovy
@@ -0,0 +1,43 @@
+/*
+ * This file holds several functions used to render the nf-core ANSI header.
+ */
+
+class Headers {
+
+    private static Map log_colours(Boolean monochrome_logs) {
+        Map colorcodes = [:]
+        colorcodes['reset']       = monochrome_logs ? '' : "\033[0m"
+        colorcodes['dim']         = monochrome_logs ? '' : "\033[2m"
+        colorcodes['black']       = monochrome_logs ? '' : "\033[0;30m"
+        colorcodes['green']       = monochrome_logs ? '' : "\033[0;32m"
+        colorcodes['yellow']      = monochrome_logs ? '' :  "\033[0;33m"
+        colorcodes['yellow_bold'] = monochrome_logs ? '' : "\033[1;93m"
+        colorcodes['blue']        = monochrome_logs ? '' : "\033[0;34m"
+        colorcodes['purple']      = monochrome_logs ? '' : "\033[0;35m"
+        colorcodes['cyan']        = monochrome_logs ? '' : "\033[0;36m"
+        colorcodes['white']       = monochrome_logs ? '' : "\033[0;37m"
+        colorcodes['red']         = monochrome_logs ? '' : "\033[1;91m"
+        return colorcodes
+    }
+
+    static String dashed_line(monochrome_logs) {
+        Map colors = log_colours(monochrome_logs)
+        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
+    }
+
+    static String nf_core(workflow, monochrome_logs) {
+        Map colors = log_colours(monochrome_logs)
+        String.format(
+            """\n
+            ${dashed_line(monochrome_logs)}
+                                                    ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
+            ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
+            ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
+            ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
+                                                    ${colors.green}`._,._,\'${colors.reset}
+            ${colors.purple}  ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset}
+            ${dashed_line(monochrome_logs)}
+            """.stripIndent()
+        )
+    }
+}
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
new file mode 100644
index 00000000..78e8c65d
--- /dev/null
+++ b/lib/NfcoreSchema.groovy
@@ -0,0 +1,571 @@
+/*
+ * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template.
+ */
+
+import org.everit.json.schema.Schema
+import org.everit.json.schema.loader.SchemaLoader
+import org.everit.json.schema.ValidationException
+import org.json.JSONObject
+import org.json.JSONTokener
+import org.json.JSONArray
+import groovy.json.JsonSlurper
+import groovy.json.JsonBuilder
+
+class NfcoreSchema {
+
+    /*
+    * Function to loop over all parameters defined in schema and check
+    * whether the given paremeters adhere to the specificiations
+    */
+    /* groovylint-disable-next-line UnusedPrivateMethodParameter */
+    private static void validateParameters(params, jsonSchema, log) {
+        def has_error = false
+        //=====================================================================//
+        // Check for nextflow core params and unexpected params
+        def json = new File(jsonSchema).text
+        def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
+        def nf_params = [
+            // Options for base `nextflow` command
+            'bg',
+            'c',
+            'C',
+            'config',
+            'd',
+            'D',
+            'dockerize',
+            'h',
+            'log',
+            'q',
+            'quiet',
+            'syslog',
+            'v',
+            'version',
+
+            // Options for `nextflow run` command
+            'ansi',
+            'ansi-log',
+            'bg',
+            'bucket-dir',
+            'c',
+            'cache',
+            'config',
+            'dsl2',
+            'dump-channels',
+            'dump-hashes',
+            'E',
+            'entry',
+            'latest',
+            'lib',
+            'main-script',
+            'N',
+            'name',
+            'offline',
+            'params-file',
+            'pi',
+            'plugins',
+            'poll-interval',
+            'pool-size',
+            'profile',
+            'ps',
+            'qs',
+            'queue-size',
+            'r',
+            'resume',
+            'revision',
+            'stdin',
+            'stub',
+            'stub-run',
+            'test',
+            'w',
+            'with-charliecloud',
+            'with-conda',
+            'with-dag',
+            'with-docker',
+            'with-mpi',
+            'with-notification',
+            'with-podman',
+            'with-report',
+            'with-singularity',
+            'with-timeline',
+            'with-tower',
+            'with-trace',
+            'with-weblog',
+            'without-docker',
+            'without-podman',
+            'work-dir'
+        ]
+        def unexpectedParams = []
+
+        // Collect expected parameters from the schema
+        def expectedParams = []
+        for (group in schemaParams) {
+            for (p in group.value['properties']) {
+                expectedParams.push(p.key)
+            }
+        }
+
+        for (specifiedParam in params.keySet()) {
+            // nextflow params
+            if (nf_params.contains(specifiedParam)) {
+                log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'"
+                has_error = true
+            }
+            // unexpected params
+            def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params'
+            if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam)) {
+                unexpectedParams.push(specifiedParam)
+            }
+        }
+
+        //=====================================================================//
+        // Validate parameters against the schema
+        InputStream inputStream = new File(jsonSchema).newInputStream()
+        JSONObject rawSchema = new JSONObject(new JSONTokener(inputStream))
+
+        // Remove anything that's in params.schema_ignore_params
+        rawSchema = removeIgnoredParams(rawSchema, params)
+
+        Schema schema = SchemaLoader.load(rawSchema)
+
+        // Clean the parameters
+        def cleanedParams = cleanParameters(params)
+
+        // Convert to JSONObject
+        def jsonParams = new JsonBuilder(cleanedParams)
+        JSONObject paramsJSON = new JSONObject(jsonParams.toString())
+
+        // Validate
+        try {
+            schema.validate(paramsJSON)
+        } catch (ValidationException e) {
+            println ''
+            log.error 'ERROR: Validation of pipeline parameters failed!'
+            JSONObject exceptionJSON = e.toJSON()
+            printExceptions(exceptionJSON, paramsJSON, log)
+            println ''
+            has_error = true
+        }
+
+        // Check for unexpected parameters
+        if (unexpectedParams.size() > 0) {
+            Map colors = log_colours(params.monochrome_logs)
+            println ''
+            def warn_msg = 'Found unexpected parameters:'
+            for (unexpectedParam in unexpectedParams) {
+                warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}"
+            }
+            log.warn warn_msg
+            log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}"
+            println ''
+        }
+
+        if (has_error) {
+            System.exit(1)
+        }
+    }
+
+    // Loop over nested exceptions and print the causingException
+    private static void printExceptions(exJSON, paramsJSON, log) {
+        def causingExceptions = exJSON['causingExceptions']
+        if (causingExceptions.length() == 0) {
+            def m = exJSON['message'] =~ /required key \[([^\]]+)\] not found/
+            // Missing required param
+            if (m.matches()) {
+                log.error "* Missing required parameter: --${m[0][1]}"
+            }
+            // Other base-level error
+            else if (exJSON['pointerToViolation'] == '#') {
+                log.error "* ${exJSON['message']}"
+            }
+            // Error with specific param
+            else {
+                def param = exJSON['pointerToViolation'] - ~/^#\//
+                def param_val = paramsJSON[param].toString()
+                log.error "* --${param}: ${exJSON['message']} (${param_val})"
+            }
+        }
+        for (ex in causingExceptions) {
+            printExceptions(ex, paramsJSON, log)
+        }
+    }
+
+    // Remove an element from a JSONArray
+    private static JSONArray removeElement(jsonArray, element){
+        def list = []  
+        int len = jsonArray.length()
+        for (int i=0;i<len;i++){ 
+            list.add(jsonArray.get(i).toString())
+        } 
+        list.remove(element)
+        JSONArray jsArray = new JSONArray(list)
+        return jsArray
+    }
+
+    private static JSONObject removeIgnoredParams(rawSchema, params){
+        // Remove anything that's in params.schema_ignore_params
+        params.schema_ignore_params.split(',').each{ ignore_param ->
+            if(rawSchema.keySet().contains('definitions')){
+                rawSchema.definitions.each { definition ->
+                    for (key in definition.keySet()){
+                        if (definition[key].get("properties").keySet().contains(ignore_param)){
+                            // Remove the param to ignore
+                            definition[key].get("properties").remove(ignore_param)
+                            // If the param was required, change this
+                            if (definition[key].has("required")) {
+                                def cleaned_required = removeElement(definition[key].required, ignore_param)
+                                definition[key].put("required", cleaned_required) 
+                            }
+                        }
+                    }
+                }
+            }
+            if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').containsKey(ignore_param)) {
+                rawSchema.get("properties").remove(ignore_param)
+            }
+            if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) {
+                def cleaned_required = removeElement(rawSchema.required, ignore_param)
+                rawSchema.put("required", cleaned_required)
+            }
+        }
+        return rawSchema
+    }
+
+    private static Map cleanParameters(params) {
+        def new_params = params.getClass().newInstance(params)
+        for (p in params) {
+            // remove anything evaluating to false
+            if (!p['value']) {
+                new_params.remove(p.key)
+            }
+            // Cast MemoryUnit to String
+            if (p['value'].getClass() == nextflow.util.MemoryUnit) {
+                new_params.replace(p.key, p['value'].toString())
+            }
+            // Cast Duration to String
+            if (p['value'].getClass() == nextflow.util.Duration) {
+                new_params.replace(p.key, p['value'].toString())
+            }
+            // Cast LinkedHashMap to String
+            if (p['value'].getClass() == LinkedHashMap) {
+                new_params.replace(p.key, p['value'].toString())
+            }
+        }
+        return new_params
+    }
+
+     /*
+     * This method tries to read a JSON params file
+     */
+    private static LinkedHashMap params_load(String json_schema) {
+        def params_map = new LinkedHashMap()
+        try {
+            params_map = params_read(json_schema)
+        } catch (Exception e) {
+            println "Could not read parameters settings from JSON. $e"
+            params_map = new LinkedHashMap()
+        }
+        return params_map
+    }
+
+    private static Map log_colours(Boolean monochrome_logs) {
+        Map colorcodes = [:]
+
+        // Reset / Meta
+        colorcodes['reset']       = monochrome_logs ? '' : "\033[0m"
+        colorcodes['bold']        = monochrome_logs ? '' : "\033[1m"
+        colorcodes['dim']         = monochrome_logs ? '' : "\033[2m"
+        colorcodes['underlined']  = monochrome_logs ? '' : "\033[4m"
+        colorcodes['blink']       = monochrome_logs ? '' : "\033[5m"
+        colorcodes['reverse']     = monochrome_logs ? '' : "\033[7m"
+        colorcodes['hidden']      = monochrome_logs ? '' : "\033[8m"
+
+        // Regular Colors
+        colorcodes['black']       = monochrome_logs ? '' : "\033[0;30m"
+        colorcodes['red']         = monochrome_logs ? '' : "\033[0;31m"
+        colorcodes['green']       = monochrome_logs ? '' : "\033[0;32m"
+        colorcodes['yellow']      = monochrome_logs ? '' : "\033[0;33m"
+        colorcodes['blue']        = monochrome_logs ? '' : "\033[0;34m"
+        colorcodes['purple']      = monochrome_logs ? '' : "\033[0;35m"
+        colorcodes['cyan']        = monochrome_logs ? '' : "\033[0;36m"
+        colorcodes['white']       = monochrome_logs ? '' : "\033[0;37m"
+
+        // Bold
+        colorcodes['bblack']      = monochrome_logs ? '' : "\033[1;30m"
+        colorcodes['bred']        = monochrome_logs ? '' : "\033[1;31m"
+        colorcodes['bgreen']      = monochrome_logs ? '' : "\033[1;32m"
+        colorcodes['byellow']     = monochrome_logs ? '' : "\033[1;33m"
+        colorcodes['bblue']       = monochrome_logs ? '' : "\033[1;34m"
+        colorcodes['bpurple']     = monochrome_logs ? '' : "\033[1;35m"
+        colorcodes['bcyan']       = monochrome_logs ? '' : "\033[1;36m"
+        colorcodes['bwhite']      = monochrome_logs ? '' : "\033[1;37m"
+
+        // Underline
+        colorcodes['ublack']      = monochrome_logs ? '' : "\033[4;30m"
+        colorcodes['ured']        = monochrome_logs ? '' : "\033[4;31m"
+        colorcodes['ugreen']      = monochrome_logs ? '' : "\033[4;32m"
+        colorcodes['uyellow']     = monochrome_logs ? '' : "\033[4;33m"
+        colorcodes['ublue']       = monochrome_logs ? '' : "\033[4;34m"
+        colorcodes['upurple']     = monochrome_logs ? '' : "\033[4;35m"
+        colorcodes['ucyan']       = monochrome_logs ? '' : "\033[4;36m"
+        colorcodes['uwhite']      = monochrome_logs ? '' : "\033[4;37m"
+
+        // High Intensity
+        colorcodes['iblack']      = monochrome_logs ? '' : "\033[0;90m"
+        colorcodes['ired']        = monochrome_logs ? '' : "\033[0;91m"
+        colorcodes['igreen']      = monochrome_logs ? '' : "\033[0;92m"
+        colorcodes['iyellow']     = monochrome_logs ? '' : "\033[0;93m"
+        colorcodes['iblue']       = monochrome_logs ? '' : "\033[0;94m"
+        colorcodes['ipurple']     = monochrome_logs ? '' : "\033[0;95m"
+        colorcodes['icyan']       = monochrome_logs ? '' : "\033[0;96m"
+        colorcodes['iwhite']      = monochrome_logs ? '' : "\033[0;97m"
+
+        // Bold High Intensity
+        colorcodes['biblack']     = monochrome_logs ? '' : "\033[1;90m"
+        colorcodes['bired']       = monochrome_logs ? '' : "\033[1;91m"
+        colorcodes['bigreen']     = monochrome_logs ? '' : "\033[1;92m"
+        colorcodes['biyellow']    = monochrome_logs ? '' : "\033[1;93m"
+        colorcodes['biblue']      = monochrome_logs ? '' : "\033[1;94m"
+        colorcodes['bipurple']    = monochrome_logs ? '' : "\033[1;95m"
+        colorcodes['bicyan']      = monochrome_logs ? '' : "\033[1;96m"
+        colorcodes['biwhite']     = monochrome_logs ? '' : "\033[1;97m"
+
+        return colorcodes
+    }
+
+    static String dashed_line(monochrome_logs) {
+        Map colors = log_colours(monochrome_logs)
+        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
+    }
+
+    /*
+    Method to actually read in JSON file using Groovy.
+    Group (as Key), values are all parameters
+        - Parameter1 as Key, Description as Value
+        - Parameter2 as Key, Description as Value
+        ....
+    Group
+        -
+    */
+    private static LinkedHashMap params_read(String json_schema) throws Exception {
+        def json = new File(json_schema).text
+        def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions')
+        def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties')
+        /* Tree looks like this in nf-core schema
+         * definitions <- this is what the first get('definitions') gets us
+             group 1
+               title
+               description
+                 properties
+                   parameter 1
+                     type
+                     description
+                   parameter 2
+                     type
+                     description
+             group 2
+               title
+               description
+                 properties
+                   parameter 1
+                     type
+                     description
+         * properties <- parameters can also be ungrouped, outside of definitions
+            parameter 1
+             type
+             description
+        */
+
+        // Grouped params
+        def params_map = new LinkedHashMap()
+        schema_definitions.each { key, val ->
+            def Map group = schema_definitions."$key".properties // Gets the property object of the group
+            def title = schema_definitions."$key".title
+            def sub_params = new LinkedHashMap()
+            group.each { innerkey, value ->
+                sub_params.put(innerkey, value)
+            }
+            params_map.put(title, sub_params)
+        }
+
+        // Ungrouped params
+        def ungrouped_params = new LinkedHashMap()
+        schema_properties.each { innerkey, value ->
+            ungrouped_params.put(innerkey, value)
+        }
+        params_map.put("Other parameters", ungrouped_params)
+
+        return params_map
+    }
+
+    /*
+     * Get maximum number of characters across all parameter names
+     */
+    private static Integer params_max_chars(params_map) {
+        Integer max_chars = 0
+        for (group in params_map.keySet()) {
+            def group_params = params_map.get(group)  // This gets the parameters of that particular group
+            for (param in group_params.keySet()) {
+                if (param.size() > max_chars) {
+                    max_chars = param.size()
+                }
+            }
+        }
+        return max_chars
+    }
+
+    /*
+     * Beautify parameters for --help
+     */
+    private static String params_help(workflow, params, json_schema, command) {
+        Map colors = log_colours(params.monochrome_logs)
+        Integer num_hidden = 0
+        String output  = ''
+        output        += 'Typical pipeline command:\n\n'
+        output        += "  ${colors.cyan}${command}${colors.reset}\n\n"
+        Map params_map = params_load(json_schema)
+        Integer max_chars  = params_max_chars(params_map) + 1
+        Integer desc_indent = max_chars + 14
+        Integer dec_linewidth = 160 - desc_indent
+        for (group in params_map.keySet()) {
+            Integer num_params = 0
+            String group_output = colors.underlined + colors.bold + group + colors.reset + '\n'
+            def group_params = params_map.get(group)  // This gets the parameters of that particular group
+            for (param in group_params.keySet()) {
+                if (group_params.get(param).hidden && !params.show_hidden_params) {
+                    num_hidden += 1
+                    continue;
+                }
+                def type = '[' + group_params.get(param).type + ']'
+                def description = group_params.get(param).description
+                def defaultValue = group_params.get(param).default ? " [default: " + group_params.get(param).default.toString() + "]" : ''
+                def description_default = description + colors.dim + defaultValue + colors.reset
+                // Wrap long description texts
+                // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap
+                if (description_default.length() > dec_linewidth){
+                    List olines = []
+                    String oline = "" // " " * indent
+                    description_default.split(" ").each() { wrd ->
+                        if ((oline.size() + wrd.size()) <= dec_linewidth) {
+                            oline += wrd + " "
+                        } else {
+                            olines += oline
+                            oline = wrd + " "
+                        }
+                    }
+                    olines += oline
+                    description_default = olines.join("\n" + " " * desc_indent)
+                }
+                group_output += "  --" +  param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n'
+                num_params += 1
+            }
+            group_output += '\n'
+            if (num_params > 0){
+                output += group_output
+            }
+        }
+        output += dashed_line(params.monochrome_logs)
+        if (num_hidden > 0){
+            output += colors.dim + "\n Hiding $num_hidden params, use --show_hidden_params to show.\n" + colors.reset
+            output += dashed_line(params.monochrome_logs)
+        }
+        return output
+    }
+
+    /*
+     * Groovy Map summarising parameters/workflow options used by the pipeline
+     */
+    private static LinkedHashMap params_summary_map(workflow, params, json_schema) {
+        // Get a selection of core Nextflow workflow options
+        def Map workflow_summary = [:]
+        if (workflow.revision) {
+            workflow_summary['revision'] = workflow.revision
+        }
+        workflow_summary['runName']      = workflow.runName
+        if (workflow.containerEngine) {
+            workflow_summary['containerEngine'] = "$workflow.containerEngine"
+        }
+        if (workflow.container) {
+            workflow_summary['container']       = "$workflow.container"
+        }
+        workflow_summary['launchDir']    = workflow.launchDir
+        workflow_summary['workDir']      = workflow.workDir
+        workflow_summary['projectDir']   = workflow.projectDir
+        workflow_summary['userName']     = workflow.userName
+        workflow_summary['profile']      = workflow.profile
+        workflow_summary['configFiles']  = workflow.configFiles.join(', ')
+
+        // Get pipeline parameters defined in JSON Schema
+        def Map params_summary = [:]
+        def blacklist  = ['hostnames']
+        def params_map = params_load(json_schema)
+        for (group in params_map.keySet()) {
+            def sub_params = new LinkedHashMap()
+            def group_params = params_map.get(group)  // This gets the parameters of that particular group
+            for (param in group_params.keySet()) {
+                if (params.containsKey(param) && !blacklist.contains(param)) {
+                    def params_value = params.get(param)
+                    def schema_value = group_params.get(param).default
+                    def param_type   = group_params.get(param).type
+                    if (schema_value == null) {
+                        if (param_type == 'boolean') {
+                            schema_value = false
+                        }
+                        if (param_type == 'string') {
+                            schema_value = ''
+                        }
+                        if (param_type == 'integer') {
+                            schema_value = 0
+                        }
+                    } else {
+                        if (param_type == 'string') {
+                            if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) {
+                                def sub_string = schema_value.replace('\$projectDir', '')
+                                sub_string     = sub_string.replace('\${projectDir}', '')
+                                if (params_value.contains(sub_string)) {
+                                    schema_value = params_value
+                                }
+                            }
+                            if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) {
+                                def sub_string = schema_value.replace('\$params.outdir', '')
+                                sub_string     = sub_string.replace('\${params.outdir}', '')
+                                if ("${params.outdir}${sub_string}" == params_value) {
+                                    schema_value = params_value
+                                }
+                            }
+                        }
+                    }
+
+                    if (params_value != schema_value) {
+                        sub_params.put("$param", params_value)
+                    }
+                }
+            }
+            params_summary.put(group, sub_params)
+        }
+        return [ 'Core Nextflow options' : workflow_summary ] << params_summary
+    }
+
+    /*
+     * Beautify parameters for summary and return as string
+     */
+    private static String params_summary_log(workflow, params, json_schema) {
+        String output  = ''
+        def params_map = params_summary_map(workflow, params, json_schema)
+        def max_chars  = params_max_chars(params_map)
+        for (group in params_map.keySet()) {
+            def group_params = params_map.get(group)  // This gets the parameters of that particular group
+            if (group_params) {
+                output += group + '\n'
+                for (param in group_params.keySet()) {
+                    output += "    \u001B[1m" +  param.padRight(max_chars) + ": \u001B[1m" + group_params.get(param) + '\n'
+                }
+                output += '\n'
+            }
+        }
+        output += "[Only displaying parameters that differ from pipeline default]\n"
+        output += dashed_line(params.monochrome_logs)
+        output += '\n\n' + dashed_line(params.monochrome_logs)
+        return output
+    }
+
+}
diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar
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literal 0
HcmV?d00001

diff --git a/main.nf b/main.nf
index 29222833..5b7ede20 100644
--- a/main.nf
+++ b/main.nf
@@ -9,57 +9,32 @@
 ----------------------------------------------------------------------------------------
 */
 
-def helpMessage() {
-    // TODO nf-core: Add to this help message with new command line parameters
-    log.info nfcoreHeader()
-    log.info"""
-
-    Usage:
-
-    The typical command for running the pipeline is as follows:
-
-    nextflow run nf-core/rnafusion --input '*_R{1,2}.fastq.gz' -profile docker
-
-    Mandatory arguments:
-      --input [file]                  Path to input data (must be surrounded with quotes)
-      -profile [str]                  Configuration profile to use. Can use multiple (comma separated)
-                                      Available: conda, docker, singularity, test, awsbatch, <institute> and more
-
-    Options:
-      --genome [str]                  Name of iGenomes reference
-      --single_end [bool]             Specifies that the input is single-end reads
-
-    References                        If not specified in the configuration file or you wish to overwrite any of the references
-      --fasta [file]                  Path to fasta reference
-
-    Other options:
-      --outdir [file]                 The output directory where the results will be saved
-      --publish_dir_mode [str]        Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy)
-      --email [email]                 Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits
-      --email_on_fail [email]         Same as --email, except only send mail if the workflow is not successful
-      --max_multiqc_email_size [str]  Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB)
-      -name [str]                     Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic
-
-    AWSBatch options:
-      --awsqueue [str]                The AWSBatch JobQueue that needs to be set when running on AWSBatch
-      --awsregion [str]               The AWS Region for your AWS Batch job to run on
-      --awscli [str]                  Path to the AWS CLI tool
-    """.stripIndent()
-}
+log.info Headers.nf_core(workflow, params.monochrome_logs)
 
-// Show help message
+////////////////////////////////////////////////////
+/* --               PRINT HELP                 -- */
+////////////////////////////////////////////////////+
+def json_schema = "$projectDir/nextflow_schema.json"
 if (params.help) {
-    helpMessage()
+    def command = "nextflow run nf-core/rnafusion --input '*_R{1,2}.fastq.gz' -profile docker"
+    log.info NfcoreSchema.params_help(workflow, params, json_schema, command)
     exit 0
 }
 
-/*
- * SET UP CONFIGURATION VARIABLES
- */
+////////////////////////////////////////////////////
+/* --         VALIDATE PARAMETERS              -- */
+////////////////////////////////////////////////////+
+if (params.validate_params) {
+    NfcoreSchema.validateParameters(params, json_schema, log)
+}
+
+////////////////////////////////////////////////////
+/* --     Collect configuration parameters     -- */
+////////////////////////////////////////////////////
 
 // Check if genome exists in the config file
 if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
-    exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}"
+    exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(', ')}"
 }
 
 // TODO nf-core: Add any reference files that are needed
@@ -73,22 +48,15 @@ if (params.genomes && params.genome && !params.genomes.containsKey(params.genome
 params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false
 if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) }
 
-// Has the run name been specified by the user?
-// this has the bonus effect of catching both -name and --name
-custom_runName = params.name
-if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) {
-    custom_runName = workflow.runName
-}
-
 // Check AWS batch settings
 if (workflow.profile.contains('awsbatch')) {
     // AWSBatch sanity checking
-    if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!"
+    if (!params.awsqueue || !params.awsregion) exit 1, 'Specify correct --awsqueue and --awsregion parameters on AWSBatch!'
     // Check outdir paths to be S3 buckets if running on AWSBatch
     // related: https://github.com/nextflow-io/nextflow/issues/813
-    if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!"
+    if (!params.outdir.startsWith('s3:')) exit 1, 'Outdir not on S3 - specify S3 Bucket to run on AWSBatch!'
     // Prevent trace files to be stored on S3 since S3 does not support rolling files.
-    if (params.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles."
+    if (params.tracedir.startsWith('s3:')) exit 1, 'Specify a local tracedir or run without trace! S3 cannot be used for tracefiles.'
 }
 
 // Stage config files
@@ -105,13 +73,13 @@ if (params.input_paths) {
         Channel
             .from(params.input_paths)
             .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] }
-            .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" }
+            .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' }
             .into { ch_read_files_fastqc; ch_read_files_trimming }
     } else {
         Channel
             .from(params.input_paths)
             .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] }
-            .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" }
+            .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' }
             .into { ch_read_files_fastqc; ch_read_files_trimming }
     }
 } else {
@@ -121,11 +89,15 @@ if (params.input_paths) {
         .into { ch_read_files_fastqc; ch_read_files_trimming }
 }
 
+////////////////////////////////////////////////////
+/* --         PRINT PARAMETER SUMMARY          -- */
+////////////////////////////////////////////////////
+log.info NfcoreSchema.params_summary_log(workflow, params, json_schema)
+
 // Header log info
-log.info nfcoreHeader()
 def summary = [:]
 if (workflow.revision) summary['Pipeline Release'] = workflow.revision
-summary['Run Name']         = custom_runName ?: workflow.runName
+summary['Run Name']         = workflow.runName
 // TODO nf-core: Report custom parameters here
 summary['Input']            = params.input
 summary['Fasta Ref']        = params.fasta
@@ -152,8 +124,6 @@ if (params.email || params.email_on_fail) {
     summary['E-mail on failure'] = params.email_on_fail
     summary['MultiQC maxsize']   = params.max_multiqc_email_size
 }
-log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n")
-log.info "-\033[2m--------------------------------------------------\033[0m-"
 
 // Check the hostnames against configured profiles
 checkHostname()
@@ -180,13 +150,13 @@ Channel.from(summary.collect{ [it.key, it.value] })
 process get_software_versions {
     publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode,
         saveAs: { filename ->
-                      if (filename.indexOf(".csv") > 0) filename
+                      if (filename.indexOf('.csv') > 0) filename
                       else null
-                }
+        }
 
     output:
     file 'software_versions_mqc.yaml' into ch_software_versions_yaml
-    file "software_versions.csv"
+    file 'software_versions.csv'
 
     script:
     // TODO nf-core: Get all tools to print their version number here
@@ -207,14 +177,14 @@ process fastqc {
     label 'process_medium'
     publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode,
         saveAs: { filename ->
-                      filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename"
-                }
+                      filename.indexOf('.zip') > 0 ? "zips/$filename" : "$filename"
+        }
 
     input:
     set val(name), file(reads) from ch_read_files_fastqc
 
     output:
-    file "*_fastqc.{zip,html}" into ch_fastqc_results
+    file '*_fastqc.{zip,html}' into ch_fastqc_results
 
     script:
     """
@@ -242,8 +212,12 @@ process multiqc {
     file "multiqc_plots"
 
     script:
-    rtitle = custom_runName ? "--title \"$custom_runName\"" : ''
-    rfilename = custom_runName ? "--filename " + custom_runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" : ''
+    rtitle = ''
+    rfilename = ''
+    if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) {
+        rtitle = "--title \"${workflow.runName}\""
+        rfilename = "--filename " + workflow.runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report"
+    }
     custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : ''
     // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time
     """
@@ -262,7 +236,7 @@ process output_documentation {
     file images from ch_output_docs_images
 
     output:
-    file "results_description.html"
+    file 'results_description.html'
 
     script:
     """
@@ -282,7 +256,7 @@ workflow.onComplete {
     }
     def email_fields = [:]
     email_fields['version'] = workflow.manifest.version
-    email_fields['runName'] = custom_runName ?: workflow.runName
+    email_fields['runName'] = workflow.runName
     email_fields['success'] = workflow.success
     email_fields['dateComplete'] = workflow.complete
     email_fields['duration'] = workflow.duration
@@ -389,28 +363,9 @@ workflow.onComplete {
 
 }
 
-
-def nfcoreHeader() {
-    // Log colors ANSI codes
-    c_black = params.monochrome_logs ? '' : "\033[0;30m";
-    c_blue = params.monochrome_logs ? '' : "\033[0;34m";
-    c_cyan = params.monochrome_logs ? '' : "\033[0;36m";
-    c_dim = params.monochrome_logs ? '' : "\033[2m";
-    c_green = params.monochrome_logs ? '' : "\033[0;32m";
-    c_purple = params.monochrome_logs ? '' : "\033[0;35m";
-    c_reset = params.monochrome_logs ? '' : "\033[0m";
-    c_white = params.monochrome_logs ? '' : "\033[0;37m";
-    c_yellow = params.monochrome_logs ? '' : "\033[0;33m";
-
-    return """    -${c_dim}--------------------------------------------------${c_reset}-
-                                            ${c_green},--.${c_black}/${c_green},-.${c_reset}
-    ${c_blue}        ___     __   __   __   ___     ${c_green}/,-._.--~\'${c_reset}
-    ${c_blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${c_yellow}}  {${c_reset}
-    ${c_blue}  | \\| |       \\__, \\__/ |  \\ |___     ${c_green}\\`-._,-`-,${c_reset}
-                                            ${c_green}`._,._,\'${c_reset}
-    ${c_purple}  nf-core/rnafusion v${workflow.manifest.version}${c_reset}
-    -${c_dim}--------------------------------------------------${c_reset}-
-    """.stripIndent()
+workflow.onError {
+    // Print unexpected parameters - easiest is to just rerun validation
+    NfcoreSchema.validateParameters(params, json_schema, log)
 }
 
 def checkHostname() {
@@ -419,15 +374,15 @@ def checkHostname() {
     def c_red = params.monochrome_logs ? '' : "\033[1;91m"
     def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m"
     if (params.hostnames) {
-        def hostname = "hostname".execute().text.trim()
+        def hostname = 'hostname'.execute().text.trim()
         params.hostnames.each { prof, hnames ->
             hnames.each { hname ->
                 if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                    log.error "====================================================\n" +
+                    log.error '====================================================\n' +
                             "  ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" +
                             "  but your machine hostname is ${c_white}'$hostname'${c_reset}\n" +
                             "  ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" +
-                            "============================================================"
+                            '============================================================'
                 }
             }
         }
diff --git a/nextflow.config b/nextflow.config
index 295d2f33..37fd2bbf 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -11,13 +11,13 @@ params {
   // Workflow flags
   // TODO nf-core: Specify your pipeline's command line flags
   genome = false
-  input = "data/*{1,2}.fastq.gz"
+  input = null
+  input_paths = null
   single_end = false
   outdir = './results'
   publish_dir_mode = 'copy'
 
   // Boilerplate options
-  name = false
   multiqc_config = false
   email = false
   email_on_fail = false
@@ -34,6 +34,9 @@ params {
   config_profile_description = false
   config_profile_contact = false
   config_profile_url = false
+  validate_params = true
+  show_hidden_params = false
+  schema_ignore_params = 'genomes,input_paths'
 
   // Defaults only, expecting to be overwritten
   max_memory = 128.GB
@@ -57,10 +60,21 @@ try {
 }
 
 profiles {
-  conda { process.conda = "$projectDir/environment.yml" }
+  conda {
+    docker.enabled = false
+    singularity.enabled = false
+    podman.enabled = false
+    shifter.enabled = false
+    charliecloud = false
+    process.conda = "$projectDir/environment.yml"
+  }
   debug { process.beforeScript = 'echo $HOSTNAME' }
   docker {
     docker.enabled = true
+    singularity.enabled = false
+    podman.enabled = false
+    shifter.enabled = false
+    charliecloud.enabled = false
     // Avoid this error:
     //   WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap.
     // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351
@@ -68,11 +82,33 @@ profiles {
     docker.runOptions = '-u \$(id -u):\$(id -g)'
   }
   singularity {
+    docker.enabled = false
     singularity.enabled = true
+    podman.enabled = false
+    shifter.enabled = false
+    charliecloud.enabled = false
     singularity.autoMounts = true
   }
   podman {
+    singularity.enabled = false
+    docker.enabled = false
     podman.enabled = true
+    shifter.enabled = false
+    charliecloud = false
+  }
+  shifter {
+    singularity.enabled = false
+    docker.enabled = false
+    podman.enabled = false
+    shifter.enabled = true
+    charliecloud.enabled = false
+  }
+  charliecloud {
+    singularity.enabled = false
+    docker.enabled = false
+    podman.enabled = false
+    shifter.enabled = false
+    charliecloud.enabled = true
   }
   test { includeConfig 'conf/test.config' }
   test_full { includeConfig 'conf/test_full.config' }
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 1a615348..470a5a5a 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -104,12 +104,12 @@
                         "move"
                     ]
                 },
-                "name": {
-                    "type": "string",
-                    "description": "Workflow name.",
-                    "fa_icon": "fas fa-fingerprint",
-                    "hidden": true,
-                    "help_text": "A custom name for the pipeline run. Unlike the core nextflow `-name` option with one hyphen this parameter can be reused multiple times, for example if using `-resume`. Passed through to steps such as MultiQC and used for things like report filenames and titles."
+                "validate_params": {
+                    "type": "boolean",
+                    "description": "Boolean whether to validate parameters against the schema at runtime",
+                    "default": true,
+                    "fa_icon": "fas fa-check-square",
+                    "hidden": true
                 },
                 "email_on_fail": {
                     "type": "string",
@@ -153,6 +153,13 @@
                     "default": "${params.outdir}/pipeline_info",
                     "fa_icon": "fas fa-cogs",
                     "hidden": true
+                },
+                "show_hidden_params": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-eye-slash",
+                    "description": "Show all params when using `--help`",
+                    "hidden": true,
+                    "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters."
                 }
             }
         },
@@ -176,6 +183,7 @@
                     "description": "Maximum amount of memory that can be requested for any single job.",
                     "default": "128.GB",
                     "fa_icon": "fas fa-memory",
+                    "pattern": "^[\\d\\.]+\\s*.(K|M|G|T)?B$",
                     "hidden": true,
                     "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`"
                 },
@@ -184,6 +192,7 @@
                     "description": "Maximum amount of time that can be requested for any single job.",
                     "default": "240.h",
                     "fa_icon": "far fa-clock",
+                    "pattern": "^[\\d\\.]+\\.*(s|m|h|d)$",
                     "hidden": true,
                     "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`"
                 }
@@ -218,6 +227,12 @@
                     "hidden": true,
                     "fa_icon": "fas fa-users-cog"
                 },
+                "config_profile_name": {
+                    "type": "string",
+                    "description": "Institutional config name.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                },
                 "config_profile_description": {
                     "type": "string",
                     "description": "Institutional config description.",

From 03db08fbd9847a74ffc10d77be422933ab222b16 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Fri, 19 Mar 2021 13:07:38 +0000
Subject: [PATCH 022/359] Template update for nf-core/tools version 1.13.1

---
 .github/workflows/branch.yml | 12 ++++++++++--
 Dockerfile                   |  2 +-
 2 files changed, 11 insertions(+), 3 deletions(-)

diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 8a6b59d2..37b7dea8 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -23,11 +23,19 @@ jobs:
         uses: mshick/add-pr-comment@v1
         with:
           message: |
+            ## This PR is against the `master` branch :x:
+
+            * Do not close this PR
+            * Click _Edit_ and change the `base` to `dev`
+            * This CI test will remain failed until you push a new commit
+
+            ---
+
             Hi @${{ github.event.pull_request.user.login }},
 
-            It looks like this pull-request is has been made against the ${{github.event.pull_request.base.repo.full_name }} `master` branch.
+            It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch.
             The `master` branch on nf-core repositories should always contain code from the latest release.
-            Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.base.repo.full_name }} `dev` branch.
+            Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
 
             You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page.
             Note that even after this, the test will continue to show as failing until you push a new commit.
diff --git a/Dockerfile b/Dockerfile
index b0562bed..05eeefc6 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.13
+FROM nfcore/base:1.13.1
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 

From 35415598d1b303a3a2b0c1dc129f9ee920087e08 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 23 Mar 2021 16:27:59 +0000
Subject: [PATCH 023/359] Template update for nf-core/tools version 1.13.2

---
 .github/workflows/ci.yml        |  2 +-
 .github/workflows/linting.yml   | 57 ++++++++++++++++++++++++++++++++-
 Dockerfile                      |  2 +-
 bin/markdown_to_html.py         |  0
 bin/scrape_software_versions.py |  0
 lib/NfcoreSchema.groovy         |  2 +-
 6 files changed, 59 insertions(+), 4 deletions(-)
 mode change 100644 => 100755 bin/markdown_to_html.py
 mode change 100644 => 100755 bin/scrape_software_versions.py

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 5b4e75cd..91ea84df 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       matrix:
         # Nextflow versions: check pipeline minimum and current latest
-        nxf_ver: ['20.04.0', '']
+        nxf_ver: ['20.04.0', '21.03.0-edge']
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 58e281df..fcde400c 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -19,6 +19,34 @@ jobs:
         run: npm install -g markdownlint-cli
       - name: Run Markdownlint
         run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml
+
+      # If the above check failed, post a comment on the PR explaining the failure
+      - name: Post PR comment
+        if: failure()
+        uses: mshick/add-pr-comment@v1
+        with:
+          message: |
+            ## Markdown linting is failing
+
+            To keep the code consistent with lots of contributors, we run automated code consistency checks.
+            To fix this CI test, please run:
+
+            * Install `markdownlint-cli`
+                * On Mac: `brew install markdownlint-cli`
+                * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`)
+            * Fix the markdown errors
+                * Automatically: `markdownlint . --config .github/markdownlint.yml --fix`
+                * Manually resolve anything left from `markdownlint . --config .github/markdownlint.yml`
+
+            Once you push these changes the test should pass, and you can hide this comment :+1:
+
+            We highly recommend setting up markdownlint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help!
+
+            Thanks again for your contribution!
+          repo-token: ${{ secrets.GITHUB_TOKEN }}
+          allow-repeats: false
+
+
   YAML:
     runs-on: ubuntu-latest
     steps:
@@ -29,7 +57,34 @@ jobs:
       - name: Install yaml-lint
         run: npm install -g yaml-lint
       - name: Run yaml-lint
-        run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml")
+        run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml" -o -name "*.yaml")
+
+      # If the above check failed, post a comment on the PR explaining the failure
+      - name: Post PR comment
+        if: failure()
+        uses: mshick/add-pr-comment@v1
+        with:
+          message: |
+            ## YAML linting is failing
+
+            To keep the code consistent with lots of contributors, we run automated code consistency checks.
+            To fix this CI test, please run:
+
+            * Install `yaml-lint`
+                * [Install `npm`](https://www.npmjs.com/get-npm) then [install `yaml-lint`](https://www.npmjs.com/package/yaml-lint) (`npm install -g yaml-lint`)
+            * Fix the markdown errors
+                * Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml")`
+                * Fix any reported errors in your YAML files
+
+            Once you push these changes the test should pass, and you can hide this comment :+1:
+
+            We highly recommend setting up yaml-lint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help!
+
+            Thanks again for your contribution!
+          repo-token: ${{ secrets.GITHUB_TOKEN }}
+          allow-repeats: false
+
+
   nf-core:
     runs-on: ubuntu-latest
     steps:
diff --git a/Dockerfile b/Dockerfile
index 05eeefc6..61d7eac8 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.13.1
+FROM nfcore/base:1.13.2
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 
diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py
old mode 100644
new mode 100755
diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
old mode 100644
new mode 100755
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index 78e8c65d..54935ec8 100644
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -219,7 +219,7 @@ class NfcoreSchema {
                     }
                 }
             }
-            if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').containsKey(ignore_param)) {
+            if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').keySet().contains(ignore_param)) {
                 rawSchema.get("properties").remove(ignore_param)
             }
             if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) {

From 1a2f636dedad666b0726364972ef65977a12ee32 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 25 Mar 2021 18:17:28 +0000
Subject: [PATCH 024/359] Template update for nf-core/tools version 1.13.3

---
 Dockerfile | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/Dockerfile b/Dockerfile
index 61d7eac8..9ca9353b 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.13.2
+FROM nfcore/base:1.13.3
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 

From d147afe1f1085f65b5d138e169f3af03bf0c6c3b Mon Sep 17 00:00:00 2001
From: MaxUlysse <max.u.garcia@gmail.com>
Date: Mon, 26 Apr 2021 14:49:57 +0200
Subject: [PATCH 025/359] feat: remove social preview image to use GitHub
 OpenGraph

---
 assets/nf-core-rnafusion_social_preview.png | Bin 47745 -> 0 bytes
 assets/nf-core-rnafusion_social_preview.svg | 446 --------------------
 2 files changed, 446 deletions(-)
 delete mode 100644 assets/nf-core-rnafusion_social_preview.png
 delete mode 100644 assets/nf-core-rnafusion_social_preview.svg

diff --git a/assets/nf-core-rnafusion_social_preview.png b/assets/nf-core-rnafusion_social_preview.png
deleted file mode 100644
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From 5c01ad0f1886a165bd54e54cf4cb0a12cfb06b8f Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 11 May 2021 11:09:51 +0000
Subject: [PATCH 026/359] Template update for nf-core/tools version 1.14

---
 .github/PULL_REQUEST_TEMPLATE.md |  7 +++--
 .github/workflows/ci.yml         |  5 ++-
 Dockerfile                       |  2 +-
 README.md                        |  2 +-
 lib/NfcoreSchema.groovy          | 54 +++++++++++++++++---------------
 main.nf                          |  4 +--
 nextflow.config                  | 16 +++++-----
 nextflow_schema.json             |  6 ++--
 8 files changed, 52 insertions(+), 44 deletions(-)

diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index fd43dd87..3ae6a061 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -10,14 +10,15 @@ Remember that PRs should be made against the dev branch, unless you're preparing
 
 Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
 -->
+<!-- markdownlint-disable ul-indent -->
 
 ## PR checklist
 
 - [ ] This comment contains a description of changes (with reason).
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
-  - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py`
-  - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
-  - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
+    - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py`
+    - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](<https://github.com/>nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+    - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint .`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 91ea84df..c7ce5021 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -8,6 +8,9 @@ on:
   release:
     types: [published]
 
+# Uncomment if we need an edge release of Nextflow again
+# env: NXF_EDGE: 1
+
 jobs:
   test:
     name: Run workflow tests
@@ -20,7 +23,7 @@ jobs:
     strategy:
       matrix:
         # Nextflow versions: check pipeline minimum and current latest
-        nxf_ver: ['20.04.0', '21.03.0-edge']
+        nxf_ver: ['20.04.0', '']
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
diff --git a/Dockerfile b/Dockerfile
index 9ca9353b..8f7d8595 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.13.3
+FROM nfcore/base:1.14
 LABEL authors="Martin Proks" \
       description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
 
diff --git a/README.md b/README.md
index 0ac143f5..3a3263af 100644
--- a/README.md
+++ b/README.md
@@ -19,7 +19,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 
 ## Quick Start
 
-1. Install [`nextflow`](https://nf-co.re/usage/installation)
+1. Install [`nextflow`](https://nf-co.re/usage/installation) (`>=20.04.0`)
 
 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
 
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index 54935ec8..52ee7304 100644
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -112,8 +112,14 @@ class NfcoreSchema {
             }
             // unexpected params
             def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params'
-            if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam)) {
-                unexpectedParams.push(specifiedParam)
+            def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() }
+            def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase()
+            if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !expectedParamsLowerCase.contains(specifiedParamLowerCase)) {
+                // Temporarily remove camelCase/camel-case params #1035
+                def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()}
+                if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){
+                    unexpectedParams.push(specifiedParam)
+                }
             }
         }
 
@@ -191,11 +197,11 @@ class NfcoreSchema {
 
     // Remove an element from a JSONArray
     private static JSONArray removeElement(jsonArray, element){
-        def list = []  
+        def list = []
         int len = jsonArray.length()
-        for (int i=0;i<len;i++){ 
+        for (int i=0;i<len;i++){
             list.add(jsonArray.get(i).toString())
-        } 
+        }
         list.remove(element)
         JSONArray jsArray = new JSONArray(list)
         return jsArray
@@ -213,7 +219,7 @@ class NfcoreSchema {
                             // If the param was required, change this
                             if (definition[key].has("required")) {
                                 def cleaned_required = removeElement(definition[key].required, ignore_param)
-                                definition[key].put("required", cleaned_required) 
+                                definition[key].put("required", cleaned_required)
                             }
                         }
                     }
@@ -243,7 +249,7 @@ class NfcoreSchema {
             }
             // Cast Duration to String
             if (p['value'].getClass() == nextflow.util.Duration) {
-                new_params.replace(p.key, p['value'].toString())
+                new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day"))
             }
             // Cast LinkedHashMap to String
             if (p['value'].getClass() == LinkedHashMap) {
@@ -482,10 +488,10 @@ class NfcoreSchema {
         }
         workflow_summary['runName']      = workflow.runName
         if (workflow.containerEngine) {
-            workflow_summary['containerEngine'] = "$workflow.containerEngine"
+            workflow_summary['containerEngine'] = workflow.containerEngine
         }
         if (workflow.container) {
-            workflow_summary['container']       = "$workflow.container"
+            workflow_summary['container'] = workflow.container
         }
         workflow_summary['launchDir']    = workflow.launchDir
         workflow_summary['workDir']      = workflow.workDir
@@ -506,17 +512,7 @@ class NfcoreSchema {
                     def params_value = params.get(param)
                     def schema_value = group_params.get(param).default
                     def param_type   = group_params.get(param).type
-                    if (schema_value == null) {
-                        if (param_type == 'boolean') {
-                            schema_value = false
-                        }
-                        if (param_type == 'string') {
-                            schema_value = ''
-                        }
-                        if (param_type == 'integer') {
-                            schema_value = 0
-                        }
-                    } else {
+                    if (schema_value != null) {
                         if (param_type == 'string') {
                             if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) {
                                 def sub_string = schema_value.replace('\$projectDir', '')
@@ -535,8 +531,13 @@ class NfcoreSchema {
                         }
                     }
 
-                    if (params_value != schema_value) {
-                        sub_params.put("$param", params_value)
+                    // We have a default in the schema, and this isn't it
+                    if (schema_value != null && params_value != schema_value) {
+                        sub_params.put(param, params_value)
+                    }
+                    // No default in the schema, and this isn't empty
+                    else if (schema_value == null && params_value != "" && params_value != null && params_value != false) {
+                        sub_params.put(param, params_value)
                     }
                 }
             }
@@ -549,22 +550,23 @@ class NfcoreSchema {
      * Beautify parameters for summary and return as string
      */
     private static String params_summary_log(workflow, params, json_schema) {
+        Map colors = log_colours(params.monochrome_logs)
         String output  = ''
         def params_map = params_summary_map(workflow, params, json_schema)
         def max_chars  = params_max_chars(params_map)
         for (group in params_map.keySet()) {
             def group_params = params_map.get(group)  // This gets the parameters of that particular group
             if (group_params) {
-                output += group + '\n'
+                output += colors.bold + group + colors.reset + '\n'
                 for (param in group_params.keySet()) {
-                    output += "    \u001B[1m" +  param.padRight(max_chars) + ": \u001B[1m" + group_params.get(param) + '\n'
+                    output += "  " + colors.blue + param.padRight(max_chars) + ": " + colors.green +  group_params.get(param) + colors.reset + '\n'
                 }
                 output += '\n'
             }
         }
-        output += "[Only displaying parameters that differ from pipeline default]\n"
         output += dashed_line(params.monochrome_logs)
-        output += '\n\n' + dashed_line(params.monochrome_logs)
+        output += colors.dim + "\n Only displaying parameters that differ from defaults.\n" + colors.reset
+        output += dashed_line(params.monochrome_logs)
         return output
     }
 
diff --git a/main.nf b/main.nf
index 5b7ede20..38c5404c 100644
--- a/main.nf
+++ b/main.nf
@@ -378,11 +378,11 @@ def checkHostname() {
         params.hostnames.each { prof, hnames ->
             hnames.each { hname ->
                 if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                    log.error '====================================================\n' +
+                    log.error "${c_red}====================================================${c_reset}\n" +
                             "  ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" +
                             "  but your machine hostname is ${c_white}'$hostname'${c_reset}\n" +
                             "  ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" +
-                            '============================================================'
+                            "${c_red}====================================================${c_reset}\n"
                 }
             }
         }
diff --git a/nextflow.config b/nextflow.config
index 37fd2bbf..603a4b94 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -25,12 +25,13 @@ params {
   plaintext_email = false
   monochrome_logs = false
   help = false
-  igenomes_base = 's3://ngi-igenomes/igenomes/'
+  igenomes_base = 's3://ngi-igenomes/igenomes'
   tracedir = "${params.outdir}/pipeline_info"
   igenomes_ignore = false
   custom_config_version = 'master'
   custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
   hostnames = false
+  config_profile_name = null
   config_profile_description = false
   config_profile_contact = false
   config_profile_url = false
@@ -65,7 +66,7 @@ profiles {
     singularity.enabled = false
     podman.enabled = false
     shifter.enabled = false
-    charliecloud = false
+    charliecloud.enabled = false
     process.conda = "$projectDir/environment.yml"
   }
   debug { process.beforeScript = 'echo $HOSTNAME' }
@@ -94,7 +95,7 @@ profiles {
     docker.enabled = false
     podman.enabled = true
     shifter.enabled = false
-    charliecloud = false
+    charliecloud.enabled = false
   }
   shifter {
     singularity.enabled = false
@@ -129,21 +130,22 @@ env {
 // Capture exit codes from upstream processes when piping
 process.shell = ['/bin/bash', '-euo', 'pipefail']
 
+def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
 timeline {
   enabled = true
-  file = "${params.tracedir}/execution_timeline.html"
+  file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html"
 }
 report {
   enabled = true
-  file = "${params.tracedir}/execution_report.html"
+  file = "${params.tracedir}/execution_report_${trace_timestamp}.html"
 }
 trace {
   enabled = true
-  file = "${params.tracedir}/execution_trace.txt"
+  file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt"
 }
 dag {
   enabled = true
-  file = "${params.tracedir}/pipeline_dag.svg"
+  file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg"
 }
 
 manifest {
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 470a5a5a..2d64558c 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -62,7 +62,7 @@
                 "igenomes_base": {
                     "type": "string",
                     "description": "Directory / URL base for iGenomes references.",
-                    "default": "s3://ngi-igenomes/igenomes/",
+                    "default": "s3://ngi-igenomes/igenomes",
                     "fa_icon": "fas fa-cloud-download-alt",
                     "hidden": true
                 },
@@ -183,7 +183,7 @@
                     "description": "Maximum amount of memory that can be requested for any single job.",
                     "default": "128.GB",
                     "fa_icon": "fas fa-memory",
-                    "pattern": "^[\\d\\.]+\\s*.(K|M|G|T)?B$",
+                    "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$",
                     "hidden": true,
                     "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`"
                 },
@@ -192,7 +192,7 @@
                     "description": "Maximum amount of time that can be requested for any single job.",
                     "default": "240.h",
                     "fa_icon": "far fa-clock",
-                    "pattern": "^[\\d\\.]+\\.*(s|m|h|d)$",
+                    "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$",
                     "hidden": true,
                     "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`"
                 }

From 048f8a6c18dd5d64ebb48bd4e8278c09a1f621fc Mon Sep 17 00:00:00 2001
From: kevinmenden <kevin.menden@t-online.de>
Date: Tue, 13 Jul 2021 10:57:24 +0200
Subject: [PATCH 027/359] Template update for nf-core/tools version 2.0

---
 .editorconfig                                 |  24 +
 .github/CONTRIBUTING.md                       |   2 +-
 .github/ISSUE_TEMPLATE/bug_report.md          |   5 +-
 .github/PULL_REQUEST_TEMPLATE.md              |   3 +-
 .github/workflows/awsfulltest.yml             |  52 +-
 .github/workflows/awstest.yml                 |  50 +-
 .github/workflows/ci.yml                      |  19 +-
 .github/workflows/linting.yml                 |  21 +-
 .github/workflows/push_dockerhub_dev.yml      |  28 -
 .github/workflows/push_dockerhub_release.yml  |  29 -
 .gitignore                                    |   1 -
 .github/markdownlint.yml => .markdownlint.yml |   2 +
 CITATIONS.md                                  |  32 +
 Dockerfile                                    |  13 -
 README.md                                     |  71 +-
 assets/samplesheet.csv                        |   3 +
 assets/schema_input.json                      |  39 +
 assets/sendmail_template.txt                  |  36 +-
 bin/check_samplesheet.py                      | 146 +++
 bin/markdown_to_html.py                       |  91 --
 bin/scrape_software_versions.py               |  44 +-
 conf/base.config                              |  94 +-
 conf/igenomes.config                          | 843 +++++++++---------
 conf/modules.config                           |  32 +
 conf/test.config                              |  47 +-
 conf/test_full.config                         |  38 +-
 docs/README.md                                |   4 +-
 docs/images/mqc_fastqc_adapter.png            | Bin 0 -> 23458 bytes
 docs/images/mqc_fastqc_counts.png             | Bin 0 -> 33918 bytes
 docs/images/mqc_fastqc_quality.png            | Bin 0 -> 55769 bytes
 docs/output.md                                |  63 +-
 docs/usage.md                                 | 214 ++++-
 environment.yml                               |  15 -
 lib/Headers.groovy                            |  43 -
 lib/NfcoreSchema.groovy                       | 528 +++++------
 lib/NfcoreTemplate.groovy                     | 266 ++++++
 lib/Utils.groovy                              |  47 +
 lib/WorkflowMain.groovy                       |  94 ++
 lib/WorkflowRnafusion.groovy                  |  59 ++
 main.nf                                       | 405 +--------
 modules.json                                  |  14 +
 modules/local/functions.nf                    |  68 ++
 modules/local/get_software_versions.nf        |  33 +
 modules/local/samplesheet_check.nf            |  31 +
 modules/nf-core/modules/fastqc/functions.nf   |  68 ++
 modules/nf-core/modules/fastqc/main.nf        |  47 +
 modules/nf-core/modules/fastqc/meta.yml       |  51 ++
 modules/nf-core/modules/multiqc/functions.nf  |  68 ++
 modules/nf-core/modules/multiqc/main.nf       |  35 +
 modules/nf-core/modules/multiqc/meta.yml      |  39 +
 nextflow.config                               | 300 ++++---
 nextflow_schema.json                          | 255 +++---
 subworkflows/local/input_check.nf             |  42 +
 workflows/rnafusion.nf                        | 141 +++
 54 files changed, 2811 insertions(+), 1884 deletions(-)
 create mode 100644 .editorconfig
 delete mode 100644 .github/workflows/push_dockerhub_dev.yml
 delete mode 100644 .github/workflows/push_dockerhub_release.yml
 rename .github/markdownlint.yml => .markdownlint.yml (90%)
 create mode 100644 CITATIONS.md
 delete mode 100644 Dockerfile
 create mode 100644 assets/samplesheet.csv
 create mode 100644 assets/schema_input.json
 create mode 100755 bin/check_samplesheet.py
 delete mode 100755 bin/markdown_to_html.py
 create mode 100644 conf/modules.config
 create mode 100755 docs/images/mqc_fastqc_adapter.png
 create mode 100755 docs/images/mqc_fastqc_counts.png
 create mode 100755 docs/images/mqc_fastqc_quality.png
 delete mode 100644 environment.yml
 delete mode 100644 lib/Headers.groovy
 mode change 100644 => 100755 lib/NfcoreSchema.groovy
 create mode 100755 lib/NfcoreTemplate.groovy
 create mode 100755 lib/Utils.groovy
 create mode 100755 lib/WorkflowMain.groovy
 create mode 100755 lib/WorkflowRnafusion.groovy
 create mode 100644 modules.json
 create mode 100644 modules/local/functions.nf
 create mode 100644 modules/local/get_software_versions.nf
 create mode 100644 modules/local/samplesheet_check.nf
 create mode 100644 modules/nf-core/modules/fastqc/functions.nf
 create mode 100644 modules/nf-core/modules/fastqc/main.nf
 create mode 100644 modules/nf-core/modules/fastqc/meta.yml
 create mode 100644 modules/nf-core/modules/multiqc/functions.nf
 create mode 100644 modules/nf-core/modules/multiqc/main.nf
 create mode 100644 modules/nf-core/modules/multiqc/meta.yml
 create mode 100644 subworkflows/local/input_check.nf
 create mode 100644 workflows/rnafusion.nf

diff --git a/.editorconfig b/.editorconfig
new file mode 100644
index 00000000..afb20bb1
--- /dev/null
+++ b/.editorconfig
@@ -0,0 +1,24 @@
+root = true
+
+[*]
+charset = utf-8
+end_of_line = lf
+insert_final_newline = true
+trim_trailing_whitespace = true
+indent_size = 4
+indent_style = space
+
+[*.{yml,yaml}]
+indent_size = 2
+
+# These files are edited and tested upstream in nf-core/modules
+[/modules/nf-core/**]
+charset = unset
+end_of_line = unset
+insert_final_newline = unset
+trim_trailing_whitespace = unset
+indent_style = unset
+indent_size = unset
+
+[/assets/email*]
+indent_size = unset
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 96741701..06a1bbd5 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -74,7 +74,7 @@ If you wish to contribute a new step, please use the following coding standards:
 7. Add sanity checks for all relevant parameters.
 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`.
 9. Do local tests that the new code works properly and as expected.
-10. Add a new test command in `.github/workflow/ci.yaml`.
+10. Add a new test command in `.github/workflow/ci.yml`.
 11. If applicable add a [MultiQC](https://https://multiqc.info/) module.
 12. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, name clean up, General Statistics Table column order, and module figures are in the right order.
 13. Optional: Add any descriptions of MultiQC report sections and output files to `docs/output.md`.
diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md
index 381c7c56..21b44f28 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.md
+++ b/.github/ISSUE_TEMPLATE/bug_report.md
@@ -18,7 +18,7 @@ Please delete this text and anything that's not relevant from the template below
 I have checked the following places for your error:
 
 - [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
-- [ ] [nf-core/rnafusion pipeline documentation](https://nf-co.re/nf-core/rnafusion/usage)
+- [ ] [nf-core/rnafusion pipeline documentation](https://nf-co.re/rnafusion/usage)
 
 ## Description of the bug
 
@@ -51,13 +51,12 @@ Have you provided the following extra information/files:
 
 ## Nextflow Installation
 
-- Version: <!-- [e.g. 19.10.0] -->
+- Version: <!-- [e.g. 21.04.0] -->
 
 ## Container engine
 
 - Engine: <!-- [e.g. Conda, Docker, Singularity, Podman, Shifter or Charliecloud] -->
 - version: <!-- [e.g. 1.0.0] -->
-- Image tag: <!-- [e.g. nfcore/rnafusion:1.0.0] -->
 
 ## Additional context
 
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 3ae6a061..3760ac7b 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -16,8 +16,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
 
 - [ ] This comment contains a description of changes (with reason).
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
-    - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py`
-    - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](<https://github.com/>nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+    - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
     - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint .`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index e404dd20..ed90a293 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -1,46 +1,34 @@
 name: nf-core AWS full size tests
 # This workflow is triggered on published releases.
-# It can be additionally triggered manually with GitHub actions workflow dispatch.
+# It can be additionally triggered manually with GitHub actions workflow dispatch button.
 # It runs the -profile 'test_full' on AWS batch
 
 on:
-  workflow_run:
-    workflows: ["nf-core Docker push (release)"]
-    types: [completed]
+  release:
+    types: [published]
   workflow_dispatch:
-
-
-env:
-  AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }}
-  AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }}
-  TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }}
-  AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }}
-  AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }}
-  AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }}
-
-
 jobs:
-  run-awstest:
+  run-tower:
     name: Run AWS full tests
     if: github.repository == 'nf-core/rnafusion'
     runs-on: ubuntu-latest
     steps:
-      - name: Setup Miniconda
-        uses: conda-incubator/setup-miniconda@v2
-        with:
-          auto-update-conda: true
-          python-version: 3.7
-      - name: Install awscli
-        run: conda install -c conda-forge awscli
-      - name: Start AWS batch job
+      - name: Launch workflow via tower
+        uses: nf-core/tower-action@master
         # TODO nf-core: You can customise AWS full pipeline tests as required
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
-        # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command
-        run: |
-          aws batch submit-job \
-            --region eu-west-1 \
-            --job-name nf-core-rnafusion \
-            --job-queue $AWS_JOB_QUEUE \
-            --job-definition $AWS_JOB_DEFINITION \
-            --container-overrides '{"command": ["nf-core/rnafusion", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/rnafusion/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/rnafusion/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'
+        
+        with:
+          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
+          bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }}
+          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
+          pipeline: ${{ github.repository }}
+          revision: ${{ github.sha }}
+          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
+          parameters: |
+            {
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
+            }
+          profiles: '[ "test_full", "aws_tower" ]'
+
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index aacabe1d..c3b9fbb4 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -1,42 +1,28 @@
 name: nf-core AWS test
-# This workflow is triggered on push to the master branch.
-# It can be additionally triggered manually with GitHub actions workflow dispatch.
-# It runs the -profile 'test' on AWS batch.
+# This workflow can be triggered manually with the GitHub actions workflow dispatch button.
+# It runs the -profile 'test' on AWS batch
 
 on:
   workflow_dispatch:
-
-
-env:
-  AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }}
-  AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }}
-  TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }}
-  AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }}
-  AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }}
-  AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }}
-
-
 jobs:
-  run-awstest:
+  run-tower:
     name: Run AWS tests
     if: github.repository == 'nf-core/rnafusion'
     runs-on: ubuntu-latest
     steps:
-      - name: Setup Miniconda
-        uses: conda-incubator/setup-miniconda@v2
+      - name: Launch workflow via tower
+        uses: nf-core/tower-action@master
+        
         with:
-          auto-update-conda: true
-          python-version: 3.7
-      - name: Install awscli
-        run: conda install -c conda-forge awscli
-      - name: Start AWS batch job
-        # TODO nf-core: You can customise CI pipeline run tests as required
-        # For example: adding multiple test runs with different parameters
-        # Remember that you can parallelise this by using strategy.matrix
-        run: |
-          aws batch submit-job \
-          --region eu-west-1 \
-          --job-name nf-core-rnafusion \
-          --job-queue $AWS_JOB_QUEUE \
-          --job-definition $AWS_JOB_DEFINITION \
-          --container-overrides '{"command": ["nf-core/rnafusion", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/rnafusion/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/rnafusion/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'
+          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
+          bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }}
+          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
+          pipeline: ${{ github.repository }}
+          revision: ${{ github.sha }}
+          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
+          parameters: |
+            {
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
+            }
+          profiles: '[ "test", "aws_tower" ]'
+
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index c7ce5021..1145652b 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -23,28 +23,11 @@ jobs:
     strategy:
       matrix:
         # Nextflow versions: check pipeline minimum and current latest
-        nxf_ver: ['20.04.0', '']
+        nxf_ver: ['21.04.0', '']
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
 
-      - name: Check if Dockerfile or Conda environment changed
-        uses: technote-space/get-diff-action@v4
-        with:
-          FILES: |
-            Dockerfile
-            environment.yml
-
-      - name: Build new docker image
-        if: env.MATCHED_FILES
-        run: docker build --no-cache . -t nfcore/rnafusion:dev
-
-      - name: Pull docker image
-        if: ${{ !env.MATCHED_FILES }}
-        run: |
-          docker pull nfcore/rnafusion:dev
-          docker tag nfcore/rnafusion:dev nfcore/rnafusion:dev
-
       - name: Install Nextflow
         env:
           CAPSULE_LOG: none
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index fcde400c..13b4fc81 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -18,7 +18,7 @@ jobs:
       - name: Install markdownlint
         run: npm install -g markdownlint-cli
       - name: Run Markdownlint
-        run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml
+        run: markdownlint .
 
       # If the above check failed, post a comment on the PR explaining the failure
       - name: Post PR comment
@@ -35,8 +35,8 @@ jobs:
                 * On Mac: `brew install markdownlint-cli`
                 * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`)
             * Fix the markdown errors
-                * Automatically: `markdownlint . --config .github/markdownlint.yml --fix`
-                * Manually resolve anything left from `markdownlint . --config .github/markdownlint.yml`
+                * Automatically: `markdownlint . --fix`
+                * Manually resolve anything left from `markdownlint .`
 
             Once you push these changes the test should pass, and you can hide this comment :+1:
 
@@ -46,6 +46,20 @@ jobs:
           repo-token: ${{ secrets.GITHUB_TOKEN }}
           allow-repeats: false
 
+  EditorConfig:
+    runs-on: ubuntu-latest
+    steps:
+      - uses: actions/checkout@v2
+
+      - uses: actions/setup-node@v1
+        with:
+          node-version: "10"
+
+      - name: Install editorconfig-checker
+        run: npm install -g editorconfig-checker
+
+      - name: Run ECLint check
+        run: editorconfig-checker -exclude README.md $(git ls-files | grep -v test)
 
   YAML:
     runs-on: ubuntu-latest
@@ -84,7 +98,6 @@ jobs:
           repo-token: ${{ secrets.GITHUB_TOKEN }}
           allow-repeats: false
 
-
   nf-core:
     runs-on: ubuntu-latest
     steps:
diff --git a/.github/workflows/push_dockerhub_dev.yml b/.github/workflows/push_dockerhub_dev.yml
deleted file mode 100644
index b970cb61..00000000
--- a/.github/workflows/push_dockerhub_dev.yml
+++ /dev/null
@@ -1,28 +0,0 @@
-name: nf-core Docker push (dev)
-# This builds the docker image and pushes it to DockerHub
-# Runs on nf-core repo releases and push event to 'dev' branch (PR merges)
-on:
-  push:
-    branches:
-      - dev
-
-jobs:
-  push_dockerhub:
-    name: Push new Docker image to Docker Hub (dev)
-    runs-on: ubuntu-latest
-    # Only run for the nf-core repo, for releases and merged PRs
-    if: ${{ github.repository == 'nf-core/rnafusion' }}
-    env:
-      DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }}
-      DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }}
-    steps:
-      - name: Check out pipeline code
-        uses: actions/checkout@v2
-
-      - name: Build new docker image
-        run: docker build --no-cache . -t nfcore/rnafusion:dev
-
-      - name: Push Docker image to DockerHub (dev)
-        run: |
-          echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
-          docker push nfcore/rnafusion:dev
diff --git a/.github/workflows/push_dockerhub_release.yml b/.github/workflows/push_dockerhub_release.yml
deleted file mode 100644
index 85b08e61..00000000
--- a/.github/workflows/push_dockerhub_release.yml
+++ /dev/null
@@ -1,29 +0,0 @@
-name: nf-core Docker push (release)
-# This builds the docker image and pushes it to DockerHub
-# Runs on nf-core repo releases and push event to 'dev' branch (PR merges)
-on:
-  release:
-    types: [published]
-
-jobs:
-  push_dockerhub:
-    name: Push new Docker image to Docker Hub (release)
-    runs-on: ubuntu-latest
-    # Only run for the nf-core repo, for releases and merged PRs
-    if: ${{ github.repository == 'nf-core/rnafusion' }}
-    env:
-      DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }}
-      DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }}
-    steps:
-      - name: Check out pipeline code
-        uses: actions/checkout@v2
-
-      - name: Build new docker image
-        run: docker build --no-cache . -t nfcore/rnafusion:latest
-
-      - name: Push Docker image to DockerHub (release)
-        run: |
-          echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin
-          docker push nfcore/rnafusion:latest
-          docker tag nfcore/rnafusion:latest nfcore/rnafusion:${{ github.event.release.tag_name }}
-          docker push nfcore/rnafusion:${{ github.event.release.tag_name }}
diff --git a/.gitignore b/.gitignore
index aa4bb5b3..5124c9ac 100644
--- a/.gitignore
+++ b/.gitignore
@@ -3,7 +3,6 @@ work/
 data/
 results/
 .DS_Store
-tests/
 testing/
 testing*
 *.pyc
diff --git a/.github/markdownlint.yml b/.markdownlint.yml
similarity index 90%
rename from .github/markdownlint.yml
rename to .markdownlint.yml
index 8d7eb53b..9e605fcf 100644
--- a/.github/markdownlint.yml
+++ b/.markdownlint.yml
@@ -1,6 +1,8 @@
 # Markdownlint configuration file
 default: true
 line-length: false
+ul-indent:
+    indent: 4
 no-duplicate-header:
     siblings_only: true
 no-inline-html:
diff --git a/CITATIONS.md b/CITATIONS.md
new file mode 100644
index 00000000..9b38bf50
--- /dev/null
+++ b/CITATIONS.md
@@ -0,0 +1,32 @@
+# nf-core/rnafusion: Citations
+
+## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/)
+
+> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031.
+
+## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/)
+
+> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311.
+
+## Pipeline tools
+
+* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
+
+* [MultiQC](https://www.ncbi.nlm.nih.gov/pubmed/27312411/)
+    > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
+
+## Software packaging/containerisation tools
+
+* [Anaconda](https://anaconda.com)
+    > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web.
+
+* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/)
+    > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506.
+
+* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/)
+    > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671.
+
+* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241)
+
+* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/)
+    > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675.
diff --git a/Dockerfile b/Dockerfile
deleted file mode 100644
index 8f7d8595..00000000
--- a/Dockerfile
+++ /dev/null
@@ -1,13 +0,0 @@
-FROM nfcore/base:1.14
-LABEL authors="Martin Proks" \
-      description="Docker image containing all software requirements for the nf-core/rnafusion pipeline"
-
-# Install the conda environment
-COPY environment.yml /
-RUN conda env create --quiet -f /environment.yml && conda clean -a
-
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-1.3.0dev/bin:$PATH
-
-# Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-1.3.0dev > nf-core-rnafusion-1.3.0dev.yml
diff --git a/README.md b/README.md
index 3a3263af..41023216 100644
--- a/README.md
+++ b/README.md
@@ -1,67 +1,69 @@
 # ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo.png)
 
-**Nextflow rnafusion analysis pipeline, part of the nf-core community.**.
+[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+CI%22)
+[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)
+[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)
+[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 
-[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions)
-[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions)
-[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A520.04.0-brightgreen.svg)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.04.0-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
+[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
+[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
+[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
 
-[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/)
-[![Docker](https://img.shields.io/docker/automated/nfcore/rnafusion.svg)](https://hub.docker.com/r/nfcore/rnafusion)
-[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?logo=slack)](https://nfcore.slack.com/channels/rnafusion)
+[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)
+[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)
+[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
 
 ## Introduction
 
 <!-- TODO nf-core: Write a 1-2 sentence summary of what data the pipeline is for and what it does -->
-**nf-core/rnafusion** is a bioinformatics best-practise analysis pipeline for
+**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for Nextflow rnafusion analysis pipeline, part of the nf-core community..
 
-The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible.
+The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
+
+<!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
+On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).
+
+## Pipeline summary
+
+<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
+
+1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 
 ## Quick Start
 
-1. Install [`nextflow`](https://nf-co.re/usage/installation) (`>=20.04.0`)
+1. Install [`Nextflow`](https://nf-co.re/usage/installation) (`>=21.04.0`)
 
 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
 
 3. Download the pipeline and test it on a minimal dataset with a single command:
 
-    ```bash
+    ```console
     nextflow run nf-core/rnafusion -profile test,<docker/singularity/podman/shifter/charliecloud/conda/institute>
     ```
 
-    > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
+    > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
+    > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, it is highly recommended to use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to pre-download all of the required containers before running the pipeline and to set the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options to be able to store and re-use the images from a central location for future pipeline runs.
+    > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
 
 4. Start running your own analysis!
 
     <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
 
-    ```bash
-    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input '*_R{1,2}.fastq.gz' --genome GRCh37
+    ```console
+    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input samplesheet.csv --genome GRCh37
     ```
 
-See [usage docs](https://nf-co.re/rnafusion/usage) for all of the available options when running the pipeline.
-
-## Pipeline Summary
-
-By default, the pipeline currently performs the following:
-
-<!-- TODO nf-core: Fill in short bullet-pointed list of default steps of pipeline -->
-
-* Sequencing quality control (`FastQC`)
-* Overall pipeline run summaries (`MultiQC`)
-
 ## Documentation
 
-The nf-core/rnafusion pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/rnafusion/usage) and [output](https://nf-co.re/rnafusion/output).
-
-<!-- TODO nf-core: Add a brief overview of what the pipeline does and how it works -->
+The nf-core/rnafusion pipeline comes with documentation about the pipeline [usage](https://nf-co.re/rnafusion/usage), [parameters](https://nf-co.re/rnafusion/parameters) and [output](https://nf-co.re/rnafusion/output).
 
 ## Credits
 
 nf-core/rnafusion was originally written by Martin Proks.
 
-We thank the following people for their extensive assistance in the development
-of this pipeline:
+We thank the following people for their extensive assistance in the development of this pipeline:
 
 <!-- TODO nf-core: If applicable, make list of people who have also contributed -->
 
@@ -73,9 +75,12 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 
 ## Citations
 
-<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi. -->
+<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->
 <!-- If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
 
+<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
+An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
+
 You can cite the `nf-core` publication as follows:
 
 > **The nf-core framework for community-curated bioinformatics pipelines.**
@@ -83,7 +88,3 @@ You can cite the `nf-core` publication as follows:
 > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.
 >
 > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).
-
-In addition, references of tools and data used in this pipeline are as follows:
-
-<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv
new file mode 100644
index 00000000..5f653ab7
--- /dev/null
+++ b/assets/samplesheet.csv
@@ -0,0 +1,3 @@
+sample,fastq_1,fastq_2
+SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz
+SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz,
diff --git a/assets/schema_input.json b/assets/schema_input.json
new file mode 100644
index 00000000..435d174a
--- /dev/null
+++ b/assets/schema_input.json
@@ -0,0 +1,39 @@
+{
+    "$schema": "http://json-schema.org/draft-07/schema",
+    "$id": "https://raw.githubusercontent.com/nf-core/rnafusion/master/assets/schema_input.json",
+    "title": "nf-core/rnafusion pipeline - params.input schema",
+    "description": "Schema for the file provided with params.input",
+    "type": "array",
+    "items": {
+        "type": "object",
+        "properties": {
+            "sample": {
+                "type": "string",
+                "pattern": "^\\S+$",
+                "errorMessage": "Sample name must be provided and cannot contain spaces"
+            },
+            "fastq_1": {
+                "type": "string",
+                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
+                "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
+            },
+            "fastq_2": {
+                "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'",
+                "anyOf": [
+                    {
+                        "type": "string",
+                        "pattern": "^\\S+\\.f(ast)?q\\.gz$"
+                    },
+                    {
+                        "type": "string",
+                        "maxLength": 0
+                    }
+                ]
+            }
+        },
+        "required": [
+            "sample",
+            "fastq_1"
+        ]
+    }
+}
diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt
index db67ce18..06728832 100644
--- a/assets/sendmail_template.txt
+++ b/assets/sendmail_template.txt
@@ -15,15 +15,15 @@ Content-ID: <nfcorepipelinelogo>
 Content-Disposition: inline; filename="nf-core-rnafusion_logo.png"
 
 <% out << new File("$projectDir/assets/nf-core-rnafusion_logo.png").
-  bytes.
-  encodeBase64().
-  toString().
-  tokenize( '\n' )*.
-  toList()*.
-  collate( 76 )*.
-  collect { it.join() }.
-  flatten().
-  join( '\n' ) %>
+    bytes.
+    encodeBase64().
+    toString().
+    tokenize( '\n' )*.
+    toList()*.
+    collate( 76 )*.
+    collect { it.join() }.
+    flatten().
+    join( '\n' ) %>
 
 <%
 if (mqcFile){
@@ -37,15 +37,15 @@ Content-ID: <mqcreport>
 Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\"
 
 ${mqcFileObj.
-  bytes.
-  encodeBase64().
-  toString().
-  tokenize( '\n' )*.
-  toList()*.
-  collate( 76 )*.
-  collect { it.join() }.
-  flatten().
-  join( '\n' )}
+    bytes.
+    encodeBase64().
+    toString().
+    tokenize( '\n' )*.
+    toList()*.
+    collate( 76 )*.
+    collect { it.join() }.
+    flatten().
+    join( '\n' )}
 """
 }}
 %>
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
new file mode 100755
index 00000000..dff05443
--- /dev/null
+++ b/bin/check_samplesheet.py
@@ -0,0 +1,146 @@
+#!/usr/bin/env python
+
+# TODO nf-core: Update the script to check the samplesheet
+# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
+
+import os
+import sys
+import errno
+import argparse
+
+
+def parse_args(args=None):
+    Description = "Reformat nf-core/rnafusion samplesheet file and check its contents."
+    Epilog = "Example usage: python check_samplesheet.py <FILE_IN> <FILE_OUT>"
+
+    parser = argparse.ArgumentParser(description=Description, epilog=Epilog)
+    parser.add_argument("FILE_IN", help="Input samplesheet file.")
+    parser.add_argument("FILE_OUT", help="Output file.")
+    return parser.parse_args(args)
+
+
+def make_dir(path):
+    if len(path) > 0:
+        try:
+            os.makedirs(path)
+        except OSError as exception:
+            if exception.errno != errno.EEXIST:
+                raise exception
+
+
+def print_error(error, context="Line", context_str=""):
+    error_str = "ERROR: Please check samplesheet -> {}".format(error)
+    if context != "" and context_str != "":
+        error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format(
+            error, context.strip(), context_str.strip()
+        )
+    print(error_str)
+    sys.exit(1)
+
+
+# TODO nf-core: Update the check_samplesheet function
+def check_samplesheet(file_in, file_out):
+    """
+    This function checks that the samplesheet follows the following structure:
+
+    sample,fastq_1,fastq_2
+    SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz
+    SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz
+    SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,
+
+    For an example see:
+    https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
+    """
+
+    sample_mapping_dict = {}
+    with open(file_in, "r") as fin:
+
+        ## Check header
+        MIN_COLS = 2
+        # TODO nf-core: Update the column names for the input samplesheet
+        HEADER = ["sample", "fastq_1", "fastq_2"]
+        header = [x.strip('"') for x in fin.readline().strip().split(",")]
+        if header[: len(HEADER)] != HEADER:
+            print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
+            sys.exit(1)
+
+        ## Check sample entries
+        for line in fin:
+            lspl = [x.strip().strip('"') for x in line.strip().split(",")]
+
+            # Check valid number of columns per row
+            if len(lspl) < len(HEADER):
+                print_error(
+                    "Invalid number of columns (minimum = {})!".format(len(HEADER)),
+                    "Line",
+                    line,
+                )
+            num_cols = len([x for x in lspl if x])
+            if num_cols < MIN_COLS:
+                print_error(
+                    "Invalid number of populated columns (minimum = {})!".format(MIN_COLS),
+                    "Line",
+                    line,
+                )
+
+            ## Check sample name entries
+            sample, fastq_1, fastq_2 = lspl[: len(HEADER)]
+            sample = sample.replace(" ", "_")
+            if not sample:
+                print_error("Sample entry has not been specified!", "Line", line)
+
+            ## Check FastQ file extension
+            for fastq in [fastq_1, fastq_2]:
+                if fastq:
+                    if fastq.find(" ") != -1:
+                        print_error("FastQ file contains spaces!", "Line", line)
+                    if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"):
+                        print_error(
+                            "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!",
+                            "Line",
+                            line,
+                        )
+
+            ## Auto-detect paired-end/single-end
+            sample_info = []  ## [single_end, fastq_1, fastq_2]
+            if sample and fastq_1 and fastq_2:  ## Paired-end short reads
+                sample_info = ["0", fastq_1, fastq_2]
+            elif sample and fastq_1 and not fastq_2:  ## Single-end short reads
+                sample_info = ["1", fastq_1, fastq_2]
+            else:
+                print_error("Invalid combination of columns provided!", "Line", line)
+
+            ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] }
+            if sample not in sample_mapping_dict:
+                sample_mapping_dict[sample] = [sample_info]
+            else:
+                if sample_info in sample_mapping_dict[sample]:
+                    print_error("Samplesheet contains duplicate rows!", "Line", line)
+                else:
+                    sample_mapping_dict[sample].append(sample_info)
+
+    ## Write validated samplesheet with appropriate columns
+    if len(sample_mapping_dict) > 0:
+        out_dir = os.path.dirname(file_out)
+        make_dir(out_dir)
+        with open(file_out, "w") as fout:
+            fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n")
+            for sample in sorted(sample_mapping_dict.keys()):
+
+                ## Check that multiple runs of the same sample are of the same datatype
+                if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]):
+                    print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample))
+
+                for idx, val in enumerate(sample_mapping_dict[sample]):
+                    fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n")
+    else:
+        print_error("No entries to process!", "Samplesheet: {}".format(file_in))
+
+
+def main(args=None):
+    args = parse_args(args)
+    check_samplesheet(args.FILE_IN, args.FILE_OUT)
+
+
+if __name__ == "__main__":
+    sys.exit(main())
diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py
deleted file mode 100755
index a26d1ff5..00000000
--- a/bin/markdown_to_html.py
+++ /dev/null
@@ -1,91 +0,0 @@
-#!/usr/bin/env python
-from __future__ import print_function
-import argparse
-import markdown
-import os
-import sys
-import io
-
-
-def convert_markdown(in_fn):
-    input_md = io.open(in_fn, mode="r", encoding="utf-8").read()
-    html = markdown.markdown(
-        "[TOC]\n" + input_md,
-        extensions=["pymdownx.extra", "pymdownx.b64", "pymdownx.highlight", "pymdownx.emoji", "pymdownx.tilde", "toc"],
-        extension_configs={
-            "pymdownx.b64": {"base_path": os.path.dirname(in_fn)},
-            "pymdownx.highlight": {"noclasses": True},
-            "toc": {"title": "Table of Contents"},
-        },
-    )
-    return html
-
-
-def wrap_html(contents):
-    header = """<!DOCTYPE html><html>
-    <head>
-        <link rel="stylesheet" href="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/css/bootstrap.min.css" integrity="sha384-ggOyR0iXCbMQv3Xipma34MD+dH/1fQ784/j6cY/iJTQUOhcWr7x9JvoRxT2MZw1T" crossorigin="anonymous">
-        <style>
-            body {
-              font-family: -apple-system,BlinkMacSystemFont,"Segoe UI",Roboto,"Helvetica Neue",Arial,"Noto Sans",sans-serif,"Apple Color Emoji","Segoe UI Emoji","Segoe UI Symbol","Noto Color Emoji";
-              padding: 3em;
-              margin-right: 350px;
-              max-width: 100%;
-            }
-            .toc {
-              position: fixed;
-              right: 20px;
-              width: 300px;
-              padding-top: 20px;
-              overflow: scroll;
-              height: calc(100% - 3em - 20px);
-            }
-            .toctitle {
-              font-size: 1.8em;
-              font-weight: bold;
-            }
-            .toc > ul {
-              padding: 0;
-              margin: 1rem 0;
-              list-style-type: none;
-            }
-            .toc > ul ul { padding-left: 20px; }
-            .toc > ul > li > a { display: none; }
-            img { max-width: 800px; }
-            pre {
-              padding: 0.6em 1em;
-            }
-            h2 {
-
-            }
-        </style>
-    </head>
-    <body>
-    <div class="container">
-    """
-    footer = """
-    </div>
-    </body>
-    </html>
-    """
-    return header + contents + footer
-
-
-def parse_args(args=None):
-    parser = argparse.ArgumentParser()
-    parser.add_argument("mdfile", type=argparse.FileType("r"), nargs="?", help="File to convert. Defaults to stdin.")
-    parser.add_argument(
-        "-o", "--out", type=argparse.FileType("w"), default=sys.stdout, help="Output file name. Defaults to stdout."
-    )
-    return parser.parse_args(args)
-
-
-def main(args=None):
-    args = parse_args(args)
-    converted_md = convert_markdown(args.mdfile.name)
-    html = wrap_html(converted_md)
-    args.out.write(html)
-
-
-if __name__ == "__main__":
-    sys.exit(main())
diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
index 981e8de6..590af3bb 100755
--- a/bin/scrape_software_versions.py
+++ b/bin/scrape_software_versions.py
@@ -1,36 +1,18 @@
 #!/usr/bin/env python
 from __future__ import print_function
-from collections import OrderedDict
-import re
+import os
 
-# TODO nf-core: Add additional regexes for new tools in process get_software_versions
-regexes = {
-    "nf-core/rnafusion": ["v_pipeline.txt", r"(\S+)"],
-    "Nextflow": ["v_nextflow.txt", r"(\S+)"],
-    "FastQC": ["v_fastqc.txt", r"FastQC v(\S+)"],
-    "MultiQC": ["v_multiqc.txt", r"multiqc, version (\S+)"],
-}
-results = OrderedDict()
-results["nf-core/rnafusion"] = '<span style="color:#999999;">N/A</span>'
-results["Nextflow"] = '<span style="color:#999999;">N/A</span>'
-results["FastQC"] = '<span style="color:#999999;">N/A</span>'
-results["MultiQC"] = '<span style="color:#999999;">N/A</span>'
+results = {}
+version_files = [x for x in os.listdir(".") if x.endswith(".version.txt")]
+for version_file in version_files:
 
-# Search each file using its regex
-for k, v in regexes.items():
-    try:
-        with open(v[0]) as x:
-            versions = x.read()
-            match = re.search(v[1], versions)
-            if match:
-                results[k] = "v{}".format(match.group(1))
-    except IOError:
-        results[k] = False
+    software = version_file.replace(".version.txt", "")
+    if software == "pipeline":
+        software = "nf-core/rnafusion"
 
-# Remove software set to false in results
-for k in list(results):
-    if not results[k]:
-        del results[k]
+    with open(version_file) as fin:
+        version = fin.read().strip()
+    results[software] = version
 
 # Dump to YAML
 print(
@@ -44,11 +26,11 @@
     <dl class="dl-horizontal">
 """
 )
-for k, v in results.items():
+for k, v in sorted(results.items()):
     print("        <dt>{}</dt><dd><samp>{}</samp></dd>".format(k, v))
 print("    </dl>")
 
 # Write out regexes as csv file:
-with open("software_versions.csv", "w") as f:
-    for k, v in results.items():
+with open("software_versions.tsv", "w") as f:
+    for k, v in sorted(results.items()):
         f.write("{}\t{}\n".format(k, v))
diff --git a/conf/base.config b/conf/base.config
index 33eaf0a7..5619f83a 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -1,51 +1,57 @@
 /*
- * -------------------------------------------------
- *  nf-core/rnafusion Nextflow base config file
- * -------------------------------------------------
- * A 'blank slate' config file, appropriate for general
- * use on most high performace compute environments.
- * Assumes that all software is installed and available
- * on the PATH. Runs in `local` mode - all jobs will be
- * run on the logged in environment.
- */
+========================================================================================
+    nf-core/rnafusion Nextflow base config file
+========================================================================================
+    A 'blank slate' config file, appropriate for general use on most high performance
+    compute environments. Assumes that all software is installed and available on
+    the PATH. Runs in `local` mode - all jobs will be run on the logged in environment.
+----------------------------------------------------------------------------------------
+*/
 
 process {
 
-  // TODO nf-core: Check the defaults for all processes
-  cpus = { check_max( 1 * task.attempt, 'cpus' ) }
-  memory = { check_max( 7.GB * task.attempt, 'memory' ) }
-  time = { check_max( 4.h * task.attempt, 'time' ) }
+    // TODO nf-core: Check the defaults for all processes
+    cpus   = { check_max( 1    * task.attempt, 'cpus'   ) }
+    memory = { check_max( 6.GB * task.attempt, 'memory' ) }
+    time   = { check_max( 4.h  * task.attempt, 'time'   ) }
 
-  errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' }
-  maxRetries = 1
-  maxErrors = '-1'
-
-  // Process-specific resource requirements
-  // NOTE - Only one of the labels below are used in the fastqc process in the main script.
-  //        If possible, it would be nice to keep the same label naming convention when
-  //        adding in your processes.
-  // TODO nf-core: Customise requirements for specific processes.
-  // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors
-  withLabel:process_low {
-    cpus = { check_max( 2 * task.attempt, 'cpus' ) }
-    memory = { check_max( 14.GB * task.attempt, 'memory' ) }
-    time = { check_max( 6.h * task.attempt, 'time' ) }
-  }
-  withLabel:process_medium {
-    cpus = { check_max( 6 * task.attempt, 'cpus' ) }
-    memory = { check_max( 42.GB * task.attempt, 'memory' ) }
-    time = { check_max( 8.h * task.attempt, 'time' ) }
-  }
-  withLabel:process_high {
-    cpus = { check_max( 12 * task.attempt, 'cpus' ) }
-    memory = { check_max( 84.GB * task.attempt, 'memory' ) }
-    time = { check_max( 10.h * task.attempt, 'time' ) }
-  }
-  withLabel:process_long {
-    time = { check_max( 20.h * task.attempt, 'time' ) }
-  }
-  withName:get_software_versions {
-    cache = false
-  }
+    errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' }
+    maxRetries    = 1
+    maxErrors     = '-1'
 
+    // Process-specific resource requirements
+    // NOTE - Please try and re-use the labels below as much as possible.
+    //        These labels are used and recognised by default in DSL2 files hosted on nf-core/modules.
+    //        If possible, it would be nice to keep the same label naming convention when
+    //        adding in your local modules too.
+    // TODO nf-core: Customise requirements for specific processes.
+    // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors
+    withLabel:process_low {
+        cpus   = { check_max( 2     * task.attempt, 'cpus'    ) }
+        memory = { check_max( 12.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 4.h   * task.attempt, 'time'    ) }
+    }
+    withLabel:process_medium {
+        cpus   = { check_max( 6     * task.attempt, 'cpus'    ) }
+        memory = { check_max( 36.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 8.h   * task.attempt, 'time'    ) }
+    }
+    withLabel:process_high {
+        cpus   = { check_max( 12    * task.attempt, 'cpus'    ) }
+        memory = { check_max( 72.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 16.h  * task.attempt, 'time'    ) }
+    }
+    withLabel:process_long {
+        time   = { check_max( 20.h  * task.attempt, 'time'    ) }
+    }
+    withLabel:process_high_memory {
+        memory = { check_max( 200.GB * task.attempt, 'memory' ) }
+    }
+    withLabel:error_ignore {
+        errorStrategy = 'ignore'
+    }
+    withLabel:error_retry {
+        errorStrategy = 'retry'
+        maxRetries    = 2
+    }
 }
diff --git a/conf/igenomes.config b/conf/igenomes.config
index 31b7ee61..855948de 100644
--- a/conf/igenomes.config
+++ b/conf/igenomes.config
@@ -1,421 +1,432 @@
 /*
- * -------------------------------------------------
- *  Nextflow config file for iGenomes paths
- * -------------------------------------------------
- * Defines reference genomes, using iGenome paths
- * Can be used by any config that customises the base
- * path using $params.igenomes_base / --igenomes_base
- */
+========================================================================================
+    Nextflow config file for iGenomes paths
+========================================================================================
+    Defines reference genomes using iGenome paths.
+    Can be used by any config that customises the base path using:
+        $params.igenomes_base / --igenomes_base
+----------------------------------------------------------------------------------------
+*/
 
 params {
-  // illumina iGenomes reference file paths
-  genomes {
-    'GRCh37' {
-      fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
-      mito_name   = "MT"
-      macs_gsize  = "2.7e9"
-      blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
+    // illumina iGenomes reference file paths
+    genomes {
+        'GRCh37' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
+        }
+        'GRCh38' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
+        }
+        'GRCm38' {
+            fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "1.87e9"
+            blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
+        }
+        'TAIR10' {
+            fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
+            mito_name   = "Mt"
+        }
+        'EB2' {
+            fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
+        }
+        'UMD3.1' {
+            fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'WBcel235' {
+            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
+            mito_name   = "MtDNA"
+            macs_gsize  = "9e7"
+        }
+        'CanFam3.1' {
+            fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'GRCz10' {
+            fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'BDGP6' {
+            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
+            mito_name   = "M"
+            macs_gsize  = "1.2e8"
+        }
+        'EquCab2' {
+            fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'EB1' {
+            fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
+        }
+        'Galgal4' {
+            fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'Gm01' {
+            fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
+        }
+        'Mmul_1' {
+            fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'IRGSP-1.0' {
+            fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
+            mito_name   = "Mt"
+        }
+        'CHIMP2.1.4' {
+            fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'Rnor_5.0' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'Rnor_6.0' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'R64-1-1' {
+            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+            macs_gsize  = "1.2e7"
+        }
+        'EF2' {
+            fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "1.21e7"
+        }
+        'Sbi1' {
+            fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
+        }
+        'Sscrofa10.2' {
+            fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'AGPv3' {
+            fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
+            mito_name   = "Mt"
+        }
+        'hg38' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
+        }
+        'hg19' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
+        }
+        'mm10' {
+            fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "1.87e9"
+            blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
+        }
+        'bosTau8' {
+            fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+        }
+        'ce10' {
+            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "9e7"
+        }
+        'canFam3' {
+            fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'danRer10' {
+            fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "1.37e9"
+        }
+        'dm6' {
+            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "1.2e8"
+        }
+        'equCab2' {
+            fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'galGal4' {
+            fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'panTro4' {
+            fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'rn6' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+        }
+        'sacCer3' {
+            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
+            readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "1.2e7"
+        }
+        'susScr3' {
+            fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
     }
-    'GRCh38' {
-      fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
-      mito_name   = "chrM"
-      macs_gsize  = "2.7e9"
-      blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-    }
-    'GRCm38' {
-      fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
-      mito_name   = "MT"
-      macs_gsize  = "1.87e9"
-      blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
-    }
-    'TAIR10' {
-      fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
-      mito_name   = "Mt"
-    }
-    'EB2' {
-      fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
-    }
-    'UMD3.1' {
-      fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
-      mito_name   = "MT"
-    }
-    'WBcel235' {
-      fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
-      mito_name   = "MtDNA"
-      macs_gsize  = "9e7"
-    }
-    'CanFam3.1' {
-      fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
-      mito_name   = "MT"
-    }
-    'GRCz10' {
-      fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
-      mito_name   = "MT"
-    }
-    'BDGP6' {
-      fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
-      mito_name   = "M"
-      macs_gsize  = "1.2e8"
-    }
-    'EquCab2' {
-      fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
-      mito_name   = "MT"
-    }
-    'EB1' {
-      fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
-    }
-    'Galgal4' {
-      fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
-      mito_name   = "MT"
-    }
-    'Gm01' {
-      fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
-    }
-    'Mmul_1' {
-      fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
-      mito_name   = "MT"
-    }
-    'IRGSP-1.0' {
-      fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
-      mito_name   = "Mt"
-    }
-    'CHIMP2.1.4' {
-      fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
-      mito_name   = "MT"
-    }
-    'Rnor_6.0' {
-      fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
-      mito_name   = "MT"
-    }
-    'R64-1-1' {
-      fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
-      mito_name   = "MT"
-      macs_gsize  = "1.2e7"
-    }
-    'EF2' {
-      fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
-      mito_name   = "MT"
-      macs_gsize  = "1.21e7"
-    }
-    'Sbi1' {
-      fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
-    }
-    'Sscrofa10.2' {
-      fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
-      mito_name   = "MT"
-    }
-    'AGPv3' {
-      fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
-      mito_name   = "Mt"
-    }
-    'hg38' {
-      fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
-      mito_name   = "chrM"
-      macs_gsize  = "2.7e9"
-      blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-    }
-    'hg19' {
-      fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
-      mito_name   = "chrM"
-      macs_gsize  = "2.7e9"
-      blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
-    }
-    'mm10' {
-      fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
-      mito_name   = "chrM"
-      macs_gsize  = "1.87e9"
-      blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
-    }
-    'bosTau8' {
-      fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
-      mito_name   = "chrM"
-    }
-    'ce10' {
-      fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
-      mito_name   = "chrM"
-      macs_gsize  = "9e7"
-    }
-    'canFam3' {
-      fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
-      mito_name   = "chrM"
-    }
-    'danRer10' {
-      fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
-      mito_name   = "chrM"
-      macs_gsize  = "1.37e9"
-    }
-    'dm6' {
-      fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
-      mito_name   = "chrM"
-      macs_gsize  = "1.2e8"
-    }
-    'equCab2' {
-      fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
-      mito_name   = "chrM"
-    }
-    'galGal4' {
-      fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
-      mito_name   = "chrM"
-    }
-    'panTro4' {
-      fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
-      mito_name   = "chrM"
-    }
-    'rn6' {
-      fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
-      mito_name   = "chrM"
-    }
-    'sacCer3' {
-      fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
-      readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
-      mito_name   = "chrM"
-      macs_gsize  = "1.2e7"
-    }
-    'susScr3' {
-      fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
-      bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
-      bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
-      star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
-      bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
-      gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
-      bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
-      readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
-      mito_name   = "chrM"
-    }
-  }
 }
diff --git a/conf/modules.config b/conf/modules.config
new file mode 100644
index 00000000..0b1bfdec
--- /dev/null
+++ b/conf/modules.config
@@ -0,0 +1,32 @@
+/*
+========================================================================================
+    Config file for defining DSL2 per module options
+========================================================================================
+    Available keys to override module options:
+        args            = Additional arguments appended to command in module.
+        args2           = Second set of arguments appended to command in module (multi-tool modules).
+        args3           = Third set of arguments appended to command in module (multi-tool modules).
+        publish_dir     = Directory to publish results.
+        publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path
+                            If publish_by_meta = true                 - Value of ${meta['id']} is appended as a directory to "publish_dir" path
+                            If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/"
+                                                                        is appended as a directory to "publish_dir" path
+                            If publish_by_meta = false / null         - No directories are appended to "publish_dir" path
+        publish_files   = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension
+                            The value of "directory" is appended to the standard "publish_dir" path as defined above.
+                            If publish_files = null (unspecified)     - All files are published.
+                            If publish_files = false                  - No files are published.
+        suffix          = File name suffix for output files.
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    modules {
+        'fastqc' {
+            args = "--quiet"
+        }
+        'multiqc' {
+            args = ""
+        }
+    }
+}
diff --git a/conf/test.config b/conf/test.config
index 15ca6fd7..331ff129 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -1,28 +1,29 @@
 /*
- * -------------------------------------------------
- *  Nextflow config file for running tests
- * -------------------------------------------------
- * Defines bundled input files and everything required
- * to run a fast and simple test. Use as follows:
- *   nextflow run nf-core/rnafusion -profile test,<docker/singularity>
- */
+========================================================================================
+    Nextflow config file for running minimal tests
+========================================================================================
+    Defines input files and everything required to run a fast and simple pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/rnafusion -profile test,<docker/singularity>
+
+----------------------------------------------------------------------------------------
+*/
 
 params {
-  config_profile_name = 'Test profile'
-  config_profile_description = 'Minimal test dataset to check pipeline function'
-  // Limit resources so that this can run on GitHub Actions
-  max_cpus = 2
-  max_memory = 6.GB
-  max_time = 48.h
+    config_profile_name        = 'Test profile'
+    config_profile_description = 'Minimal test dataset to check pipeline function'
+
+    // Limit resources so that this can run on GitHub Actions
+    max_cpus   = 2
+    max_memory = 6.GB
+    max_time   = 6.h
+
+    // Input data
+    // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
+    // TODO nf-core: Give any required params for the test so that command line flags are not needed
+    input  = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
 
-  // Input data
-  // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
-  // TODO nf-core: Give any required params for the test so that command line flags are not needed
-  single_end = false
-  input_paths = [
-    ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']],
-    ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']]
-  ]
-  // Ignore `--input` as otherwise the parameter validation will throw an error
-  schema_ignore_params = 'genomes,input_paths,input'
+    // Genome references
+    genome = 'R64-1-1'
 }
diff --git a/conf/test_full.config b/conf/test_full.config
index 91c0c736..260cb93f 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -1,24 +1,24 @@
 /*
- * -------------------------------------------------
- *  Nextflow config file for running full-size tests
- * -------------------------------------------------
- * Defines bundled input files and everything required
- * to run a full size pipeline test. Use as follows:
- *   nextflow run nf-core/rnafusion -profile test_full,<docker/singularity>
- */
+========================================================================================
+    Nextflow config file for running full-size tests
+========================================================================================
+    Defines input files and everything required to run a full size pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/rnafusion -profile test_full,<docker/singularity>
+
+----------------------------------------------------------------------------------------
+*/
 
 params {
-  config_profile_name = 'Full test profile'
-  config_profile_description = 'Full test dataset to check pipeline function'
+    config_profile_name        = 'Full test profile'
+    config_profile_description = 'Full test dataset to check pipeline function'
+
+    // Input data for full size test
+    // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA)
+    // TODO nf-core: Give any required params for the test so that command line flags are not needed
+    input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv'
 
-  // Input data for full size test
-  // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA)
-  // TODO nf-core: Give any required params for the test so that command line flags are not needed
-  single_end = false
-  input_paths = [
-    ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']],
-    ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']]
-  ]
-  // Ignore `--input` as otherwise the parameter validation will throw an error
-  schema_ignore_params = 'genomes,input_paths,input'
+    // Genome references
+    genome = 'R64-1-1'
 }
diff --git a/docs/README.md b/docs/README.md
index e8198d8c..08dec85e 100644
--- a/docs/README.md
+++ b/docs/README.md
@@ -3,8 +3,8 @@
 The nf-core/rnafusion documentation is split into the following pages:
 
 * [Usage](usage.md)
-  * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
+    * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
 * [Output](output.md)
-  * An overview of the different results produced by the pipeline and how to interpret them.
+    * An overview of the different results produced by the pipeline and how to interpret them.
 
 You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re)
diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png
new file mode 100755
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diff --git a/docs/output.md b/docs/output.md
index a3a11a12..8a3fc362 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -10,50 +10,59 @@ The directories listed below will be created in the results directory after the
 
 ## Pipeline overview
 
-The pipeline is built using [Nextflow](https://www.nextflow.io/)
-and processes data using the following steps:
+The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
-* [FastQC](#fastqc) - Read quality control
-* [MultiQC](#multiqc) - Aggregate report describing results from the whole pipeline
+* [FastQC](#fastqc) - Raw read QC
+* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
 * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
-## FastQC
+### FastQC
 
-[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences.
+<details markdown="1">
+<summary>Output files</summary>
 
-For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
+* `fastqc/`
+    * `*_fastqc.html`: FastQC report containing quality metrics.
+    * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
-**Output files:**
+</details>
 
-* `fastqc/`
-  * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files.
-* `fastqc/zips/`
-  * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
+[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
 
-> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
+![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png)
 
-## MultiQC
+![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png)
 
-[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarizing all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.
+![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png)
 
-The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability.
+> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
 
-For more information about how to use MultiQC reports, see [https://multiqc.info](https://multiqc.info).
+### MultiQC
 
-**Output files:**
+<details markdown="1">
+<summary>Output files</summary>
 
 * `multiqc/`
-  * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
-  * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
-  * `multiqc_plots/`: directory containing static images from the report in various formats.
+    * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
+    * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
+    * `multiqc_plots/`: directory containing static images from the report in various formats.
 
-## Pipeline information
+</details>
 
-[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
+[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.
 
-**Output files:**
+Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.
+
+### Pipeline information
+
+<details markdown="1">
+<summary>Output files</summary>
 
 * `pipeline_info/`
-  * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
-  * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`.
-  * Documentation for interpretation of results in HTML format: `results_description.html`.
+    * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
+    * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.tsv`.
+    * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
+
+</details>
+
+[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
diff --git a/docs/usage.md b/docs/usage.md
index 3be033f0..7f14f65b 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -8,19 +8,63 @@
 
 <!-- TODO nf-core: Add documentation about anything specific to running your pipeline. For general topics, please point to (and add to) the main nf-core website. -->
 
+## Samplesheet input
+
+You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below.
+
+```console
+--input '[path to samplesheet file]'
+```
+
+### Multiple runs of the same sample
+
+The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes:
+
+```console
+sample,fastq_1,fastq_2
+CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
+CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz
+CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz
+```
+
+### Full samplesheet
+
+The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below.
+
+A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice.
+
+```console
+sample,fastq_1,fastq_2
+CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
+CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz
+CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz
+TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz,
+TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz,
+TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,
+TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,
+```
+
+| Column         | Description                                                                                                                                                                            |
+|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
+| `sample`       | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). |
+| `fastq_1`      | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
+| `fastq_2`      | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
+
+An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline.
+
 ## Running the pipeline
 
 The typical command for running the pipeline is as follows:
 
-```bash
-nextflow run nf-core/rnafusion --input '*_R{1,2}.fastq.gz' -profile docker
+```console
+nextflow run nf-core/rnafusion --input samplesheet.csv --genome GRCh37 -profile docker
 ```
 
 This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
 
 Note that the pipeline will create the following files in your working directory:
 
-```bash
+```console
 work            # Directory containing the nextflow working files
 results         # Finished results (configurable, see below)
 .nextflow_log   # Log file from Nextflow
@@ -31,13 +75,13 @@ results         # Finished results (configurable, see below)
 
 When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:
 
-```bash
+```console
 nextflow pull nf-core/rnafusion
 ```
 
 ### Reproducibility
 
-It's a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since.
+It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since.
 
 First, go to the [nf-core/rnafusion releases page](https://github.com/nf-core/rnafusion/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`.
 
@@ -51,7 +95,7 @@ This version number will be logged in reports when you run the pipeline, so that
 
 Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments.
 
-Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below.
+Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. When using Biocontainers, most of these software packaging methods pull Docker containers from quay.io e.g [FastQC](https://quay.io/repository/biocontainers/fastqc) except for Singularity which directly downloads Singularity images via https hosted by the [Galaxy project](https://depot.galaxyproject.org/singularity/) and Conda which downloads and installs software locally from [Bioconda](https://bioconda.github.io/).
 
 > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
 
@@ -63,27 +107,20 @@ They are loaded in sequence, so later profiles can overwrite earlier profiles.
 If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended.
 
 * `docker`
-  * A generic configuration profile to be used with [Docker](https://docker.com/)
-  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+    * A generic configuration profile to be used with [Docker](https://docker.com/)
 * `singularity`
-  * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
-  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+    * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
 * `podman`
-  * A generic configuration profile to be used with [Podman](https://podman.io/)
-  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+    * A generic configuration profile to be used with [Podman](https://podman.io/)
 * `shifter`
-  * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
-  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+    * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
 * `charliecloud`
-  * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
-  * Pulls software from Docker Hub: [`nfcore/rnafusion`](https://hub.docker.com/r/nfcore/rnafusion/)
+    * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
 * `conda`
-  * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
-  * A generic configuration profile to be used with [Conda](https://conda.io/docs/)
-  * Pulls most software from [Bioconda](https://bioconda.github.io/)
+    * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
 * `test`
-  * A profile with a complete configuration for automated testing
-  * Includes links to test data so needs no other parameters
+    * A profile with a complete configuration for automated testing
+    * Includes links to test data so needs no other parameters
 
 ### `-resume`
 
@@ -95,29 +132,140 @@ You can also supply a run name to resume a specific run: `-resume [run-name]`. U
 
 Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information.
 
-#### Custom resource requests
+## Custom configuration
+
+### Resource requests
+
+Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped.
+
+For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue:
+
+```console
+[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
+Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
+
+Caused by:
+    Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
 
-Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped.
+Command executed:
+    STAR \
+        --genomeDir star \
+        --readFilesIn WT_REP1_trimmed.fq.gz  \
+        --runThreadN 2 \
+        --outFileNamePrefix WT_REP1. \
+        <TRUNCATED>
 
-Whilst these default requirements will hopefully work for most people with most data, you may find that you want to customise the compute resources that the pipeline requests. You can do this by creating a custom config file. For example, to give the workflow process `star` 32GB of memory, you could use the following config:
+Command exit status:
+    137
+
+Command output:
+    (empty)
+
+Command error:
+    .command.sh: line 9:  30 Killed    STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. <TRUNCATED>
+Work dir:
+    /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb
+
+Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
+```
+
+To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
 
 ```nextflow
 process {
-  withName: star {
-    memory = 32.GB
-  }
+    withName: STAR_ALIGN {
+        memory = 100.GB
+    }
 }
 ```
 
-To find the exact name of a process you wish to modify the compute resources, check the live-status of a nextflow run displayed on your terminal or check the nextflow error for a line like so: `Error executing process > 'bwa'`. In this case the name to specify in the custom config file is `bwa`.
+> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow.
+
+### Tool-specific options
+
+For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes.
+
+The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of:
+
+* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file.
+
+* An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option.
+
+The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments.
+
+When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`.
+
+As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file.
+
+Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config.
+
+As you will see in the example below, we have:
+
+* appended `--outFilterMismatchNmax 16` to the default `args` used by the module.
+* changed the default `publish_dir` value to where the files will eventually be published in the main results directory.
+* appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`.
+
+```nextflow
+params {
+    modules {
+        'star_align' {
+            args          = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16"
+            publish_dir   = "my_star_directory"
+            publish_files = ['out':'log', 'tab':'log', 'bam':'']
+        }
+    }
+}
+```
+
+### Updating containers
+
+The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`.
+
+1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19)
+2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
+3. Create the custom config accordingly:
+
+    * For Docker:
+
+        ```nextflow
+        process {
+            withName: PANGOLIN {
+                container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0'
+            }
+        }
+        ```
+
+    * For Singularity:
+
+        ```nextflow
+        process {
+            withName: PANGOLIN {
+                container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0'
+            }
+        }
+        ```
+
+    * For Conda:
+
+        ```nextflow
+        process {
+            withName: PANGOLIN {
+                conda = 'bioconda::pangolin=3.0.5'
+            }
+        }
+        ```
+
+> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch.
+
+### nf-core/configs
 
-See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information.
+In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile.
 
-If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition above). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile.
+See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files.
 
 If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs).
 
-### Running in the background
+## Running in the background
 
 Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished.
 
@@ -126,11 +274,11 @@ The Nextflow `-bg` flag launches Nextflow in the background, detached from your
 Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time.
 Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs).
 
-#### Nextflow memory requirements
+## Nextflow memory requirements
 
 In some cases, the Nextflow Java virtual machines can start to request a large amount of memory.
 We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`):
 
-```bash
+```console
 NXF_OPTS='-Xms1g -Xmx4g'
 ```
diff --git a/environment.yml b/environment.yml
deleted file mode 100644
index 162bdacd..00000000
--- a/environment.yml
+++ /dev/null
@@ -1,15 +0,0 @@
-# You can use this file to create a conda environment for this pipeline:
-#   conda env create -f environment.yml
-name: nf-core-rnafusion-1.3.0dev
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - conda-forge::python=3.7.3
-  - conda-forge::markdown=3.1.1
-  - conda-forge::pymdown-extensions=6.0
-  - conda-forge::pygments=2.5.2
-  # TODO nf-core: Add required software dependencies here
-  - bioconda::fastqc=0.11.8
-  - bioconda::multiqc=1.7
diff --git a/lib/Headers.groovy b/lib/Headers.groovy
deleted file mode 100644
index 15d1d388..00000000
--- a/lib/Headers.groovy
+++ /dev/null
@@ -1,43 +0,0 @@
-/*
- * This file holds several functions used to render the nf-core ANSI header.
- */
-
-class Headers {
-
-    private static Map log_colours(Boolean monochrome_logs) {
-        Map colorcodes = [:]
-        colorcodes['reset']       = monochrome_logs ? '' : "\033[0m"
-        colorcodes['dim']         = monochrome_logs ? '' : "\033[2m"
-        colorcodes['black']       = monochrome_logs ? '' : "\033[0;30m"
-        colorcodes['green']       = monochrome_logs ? '' : "\033[0;32m"
-        colorcodes['yellow']      = monochrome_logs ? '' :  "\033[0;33m"
-        colorcodes['yellow_bold'] = monochrome_logs ? '' : "\033[1;93m"
-        colorcodes['blue']        = monochrome_logs ? '' : "\033[0;34m"
-        colorcodes['purple']      = monochrome_logs ? '' : "\033[0;35m"
-        colorcodes['cyan']        = monochrome_logs ? '' : "\033[0;36m"
-        colorcodes['white']       = monochrome_logs ? '' : "\033[0;37m"
-        colorcodes['red']         = monochrome_logs ? '' : "\033[1;91m"
-        return colorcodes
-    }
-
-    static String dashed_line(monochrome_logs) {
-        Map colors = log_colours(monochrome_logs)
-        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
-    }
-
-    static String nf_core(workflow, monochrome_logs) {
-        Map colors = log_colours(monochrome_logs)
-        String.format(
-            """\n
-            ${dashed_line(monochrome_logs)}
-                                                    ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
-            ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
-            ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
-            ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
-                                                    ${colors.green}`._,._,\'${colors.reset}
-            ${colors.purple}  ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset}
-            ${dashed_line(monochrome_logs)}
-            """.stripIndent()
-        )
-    }
-}
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
old mode 100644
new mode 100755
index 52ee7304..8d6920dd
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -1,6 +1,6 @@
-/*
- * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template.
- */
+//
+// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template.
+//
 
 import org.everit.json.schema.Schema
 import org.everit.json.schema.loader.SchemaLoader
@@ -13,16 +13,23 @@ import groovy.json.JsonBuilder
 
 class NfcoreSchema {
 
-    /*
-    * Function to loop over all parameters defined in schema and check
-    * whether the given paremeters adhere to the specificiations
-    */
+    //
+    // Resolve Schema path relative to main workflow directory
+    //
+    public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') {
+        return "${workflow.projectDir}/${schema_filename}"
+    }
+
+    //
+    // Function to loop over all parameters defined in schema and check
+    // whether the given parameters adhere to the specifications
+    //
     /* groovylint-disable-next-line UnusedPrivateMethodParameter */
-    private static void validateParameters(params, jsonSchema, log) {
+    public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') {
         def has_error = false
         //=====================================================================//
         // Check for nextflow core params and unexpected params
-        def json = new File(jsonSchema).text
+        def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text
         def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
         def nf_params = [
             // Options for base `nextflow` command
@@ -114,7 +121,8 @@ class NfcoreSchema {
             def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params'
             def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() }
             def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase()
-            if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !expectedParamsLowerCase.contains(specifiedParamLowerCase)) {
+            def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase))
+            if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) {
                 // Temporarily remove camelCase/camel-case params #1035
                 def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()}
                 if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){
@@ -125,36 +133,36 @@ class NfcoreSchema {
 
         //=====================================================================//
         // Validate parameters against the schema
-        InputStream inputStream = new File(jsonSchema).newInputStream()
-        JSONObject rawSchema = new JSONObject(new JSONTokener(inputStream))
+        InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream()
+        JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream))
 
         // Remove anything that's in params.schema_ignore_params
-        rawSchema = removeIgnoredParams(rawSchema, params)
+        raw_schema = removeIgnoredParams(raw_schema, params)
 
-        Schema schema = SchemaLoader.load(rawSchema)
+        Schema schema = SchemaLoader.load(raw_schema)
 
         // Clean the parameters
         def cleanedParams = cleanParameters(params)
 
         // Convert to JSONObject
         def jsonParams = new JsonBuilder(cleanedParams)
-        JSONObject paramsJSON = new JSONObject(jsonParams.toString())
+        JSONObject params_json = new JSONObject(jsonParams.toString())
 
         // Validate
         try {
-            schema.validate(paramsJSON)
+            schema.validate(params_json)
         } catch (ValidationException e) {
             println ''
             log.error 'ERROR: Validation of pipeline parameters failed!'
             JSONObject exceptionJSON = e.toJSON()
-            printExceptions(exceptionJSON, paramsJSON, log)
+            printExceptions(exceptionJSON, params_json, log)
             println ''
             has_error = true
         }
 
         // Check for unexpected parameters
         if (unexpectedParams.size() > 0) {
-            Map colors = log_colours(params.monochrome_logs)
+            Map colors = NfcoreTemplate.logColours(params.monochrome_logs)
             println ''
             def warn_msg = 'Found unexpected parameters:'
             for (unexpectedParam in unexpectedParams) {
@@ -170,266 +178,17 @@ class NfcoreSchema {
         }
     }
 
-    // Loop over nested exceptions and print the causingException
-    private static void printExceptions(exJSON, paramsJSON, log) {
-        def causingExceptions = exJSON['causingExceptions']
-        if (causingExceptions.length() == 0) {
-            def m = exJSON['message'] =~ /required key \[([^\]]+)\] not found/
-            // Missing required param
-            if (m.matches()) {
-                log.error "* Missing required parameter: --${m[0][1]}"
-            }
-            // Other base-level error
-            else if (exJSON['pointerToViolation'] == '#') {
-                log.error "* ${exJSON['message']}"
-            }
-            // Error with specific param
-            else {
-                def param = exJSON['pointerToViolation'] - ~/^#\//
-                def param_val = paramsJSON[param].toString()
-                log.error "* --${param}: ${exJSON['message']} (${param_val})"
-            }
-        }
-        for (ex in causingExceptions) {
-            printExceptions(ex, paramsJSON, log)
-        }
-    }
-
-    // Remove an element from a JSONArray
-    private static JSONArray removeElement(jsonArray, element){
-        def list = []
-        int len = jsonArray.length()
-        for (int i=0;i<len;i++){
-            list.add(jsonArray.get(i).toString())
-        }
-        list.remove(element)
-        JSONArray jsArray = new JSONArray(list)
-        return jsArray
-    }
-
-    private static JSONObject removeIgnoredParams(rawSchema, params){
-        // Remove anything that's in params.schema_ignore_params
-        params.schema_ignore_params.split(',').each{ ignore_param ->
-            if(rawSchema.keySet().contains('definitions')){
-                rawSchema.definitions.each { definition ->
-                    for (key in definition.keySet()){
-                        if (definition[key].get("properties").keySet().contains(ignore_param)){
-                            // Remove the param to ignore
-                            definition[key].get("properties").remove(ignore_param)
-                            // If the param was required, change this
-                            if (definition[key].has("required")) {
-                                def cleaned_required = removeElement(definition[key].required, ignore_param)
-                                definition[key].put("required", cleaned_required)
-                            }
-                        }
-                    }
-                }
-            }
-            if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').keySet().contains(ignore_param)) {
-                rawSchema.get("properties").remove(ignore_param)
-            }
-            if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) {
-                def cleaned_required = removeElement(rawSchema.required, ignore_param)
-                rawSchema.put("required", cleaned_required)
-            }
-        }
-        return rawSchema
-    }
-
-    private static Map cleanParameters(params) {
-        def new_params = params.getClass().newInstance(params)
-        for (p in params) {
-            // remove anything evaluating to false
-            if (!p['value']) {
-                new_params.remove(p.key)
-            }
-            // Cast MemoryUnit to String
-            if (p['value'].getClass() == nextflow.util.MemoryUnit) {
-                new_params.replace(p.key, p['value'].toString())
-            }
-            // Cast Duration to String
-            if (p['value'].getClass() == nextflow.util.Duration) {
-                new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day"))
-            }
-            // Cast LinkedHashMap to String
-            if (p['value'].getClass() == LinkedHashMap) {
-                new_params.replace(p.key, p['value'].toString())
-            }
-        }
-        return new_params
-    }
-
-     /*
-     * This method tries to read a JSON params file
-     */
-    private static LinkedHashMap params_load(String json_schema) {
-        def params_map = new LinkedHashMap()
-        try {
-            params_map = params_read(json_schema)
-        } catch (Exception e) {
-            println "Could not read parameters settings from JSON. $e"
-            params_map = new LinkedHashMap()
-        }
-        return params_map
-    }
-
-    private static Map log_colours(Boolean monochrome_logs) {
-        Map colorcodes = [:]
-
-        // Reset / Meta
-        colorcodes['reset']       = monochrome_logs ? '' : "\033[0m"
-        colorcodes['bold']        = monochrome_logs ? '' : "\033[1m"
-        colorcodes['dim']         = monochrome_logs ? '' : "\033[2m"
-        colorcodes['underlined']  = monochrome_logs ? '' : "\033[4m"
-        colorcodes['blink']       = monochrome_logs ? '' : "\033[5m"
-        colorcodes['reverse']     = monochrome_logs ? '' : "\033[7m"
-        colorcodes['hidden']      = monochrome_logs ? '' : "\033[8m"
-
-        // Regular Colors
-        colorcodes['black']       = monochrome_logs ? '' : "\033[0;30m"
-        colorcodes['red']         = monochrome_logs ? '' : "\033[0;31m"
-        colorcodes['green']       = monochrome_logs ? '' : "\033[0;32m"
-        colorcodes['yellow']      = monochrome_logs ? '' : "\033[0;33m"
-        colorcodes['blue']        = monochrome_logs ? '' : "\033[0;34m"
-        colorcodes['purple']      = monochrome_logs ? '' : "\033[0;35m"
-        colorcodes['cyan']        = monochrome_logs ? '' : "\033[0;36m"
-        colorcodes['white']       = monochrome_logs ? '' : "\033[0;37m"
-
-        // Bold
-        colorcodes['bblack']      = monochrome_logs ? '' : "\033[1;30m"
-        colorcodes['bred']        = monochrome_logs ? '' : "\033[1;31m"
-        colorcodes['bgreen']      = monochrome_logs ? '' : "\033[1;32m"
-        colorcodes['byellow']     = monochrome_logs ? '' : "\033[1;33m"
-        colorcodes['bblue']       = monochrome_logs ? '' : "\033[1;34m"
-        colorcodes['bpurple']     = monochrome_logs ? '' : "\033[1;35m"
-        colorcodes['bcyan']       = monochrome_logs ? '' : "\033[1;36m"
-        colorcodes['bwhite']      = monochrome_logs ? '' : "\033[1;37m"
-
-        // Underline
-        colorcodes['ublack']      = monochrome_logs ? '' : "\033[4;30m"
-        colorcodes['ured']        = monochrome_logs ? '' : "\033[4;31m"
-        colorcodes['ugreen']      = monochrome_logs ? '' : "\033[4;32m"
-        colorcodes['uyellow']     = monochrome_logs ? '' : "\033[4;33m"
-        colorcodes['ublue']       = monochrome_logs ? '' : "\033[4;34m"
-        colorcodes['upurple']     = monochrome_logs ? '' : "\033[4;35m"
-        colorcodes['ucyan']       = monochrome_logs ? '' : "\033[4;36m"
-        colorcodes['uwhite']      = monochrome_logs ? '' : "\033[4;37m"
-
-        // High Intensity
-        colorcodes['iblack']      = monochrome_logs ? '' : "\033[0;90m"
-        colorcodes['ired']        = monochrome_logs ? '' : "\033[0;91m"
-        colorcodes['igreen']      = monochrome_logs ? '' : "\033[0;92m"
-        colorcodes['iyellow']     = monochrome_logs ? '' : "\033[0;93m"
-        colorcodes['iblue']       = monochrome_logs ? '' : "\033[0;94m"
-        colorcodes['ipurple']     = monochrome_logs ? '' : "\033[0;95m"
-        colorcodes['icyan']       = monochrome_logs ? '' : "\033[0;96m"
-        colorcodes['iwhite']      = monochrome_logs ? '' : "\033[0;97m"
-
-        // Bold High Intensity
-        colorcodes['biblack']     = monochrome_logs ? '' : "\033[1;90m"
-        colorcodes['bired']       = monochrome_logs ? '' : "\033[1;91m"
-        colorcodes['bigreen']     = monochrome_logs ? '' : "\033[1;92m"
-        colorcodes['biyellow']    = monochrome_logs ? '' : "\033[1;93m"
-        colorcodes['biblue']      = monochrome_logs ? '' : "\033[1;94m"
-        colorcodes['bipurple']    = monochrome_logs ? '' : "\033[1;95m"
-        colorcodes['bicyan']      = monochrome_logs ? '' : "\033[1;96m"
-        colorcodes['biwhite']     = monochrome_logs ? '' : "\033[1;97m"
-
-        return colorcodes
-    }
-
-    static String dashed_line(monochrome_logs) {
-        Map colors = log_colours(monochrome_logs)
-        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
-    }
-
-    /*
-    Method to actually read in JSON file using Groovy.
-    Group (as Key), values are all parameters
-        - Parameter1 as Key, Description as Value
-        - Parameter2 as Key, Description as Value
-        ....
-    Group
-        -
-    */
-    private static LinkedHashMap params_read(String json_schema) throws Exception {
-        def json = new File(json_schema).text
-        def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions')
-        def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties')
-        /* Tree looks like this in nf-core schema
-         * definitions <- this is what the first get('definitions') gets us
-             group 1
-               title
-               description
-                 properties
-                   parameter 1
-                     type
-                     description
-                   parameter 2
-                     type
-                     description
-             group 2
-               title
-               description
-                 properties
-                   parameter 1
-                     type
-                     description
-         * properties <- parameters can also be ungrouped, outside of definitions
-            parameter 1
-             type
-             description
-        */
-
-        // Grouped params
-        def params_map = new LinkedHashMap()
-        schema_definitions.each { key, val ->
-            def Map group = schema_definitions."$key".properties // Gets the property object of the group
-            def title = schema_definitions."$key".title
-            def sub_params = new LinkedHashMap()
-            group.each { innerkey, value ->
-                sub_params.put(innerkey, value)
-            }
-            params_map.put(title, sub_params)
-        }
-
-        // Ungrouped params
-        def ungrouped_params = new LinkedHashMap()
-        schema_properties.each { innerkey, value ->
-            ungrouped_params.put(innerkey, value)
-        }
-        params_map.put("Other parameters", ungrouped_params)
-
-        return params_map
-    }
-
-    /*
-     * Get maximum number of characters across all parameter names
-     */
-    private static Integer params_max_chars(params_map) {
-        Integer max_chars = 0
-        for (group in params_map.keySet()) {
-            def group_params = params_map.get(group)  // This gets the parameters of that particular group
-            for (param in group_params.keySet()) {
-                if (param.size() > max_chars) {
-                    max_chars = param.size()
-                }
-            }
-        }
-        return max_chars
-    }
-
-    /*
-     * Beautify parameters for --help
-     */
-    private static String params_help(workflow, params, json_schema, command) {
-        Map colors = log_colours(params.monochrome_logs)
+    //
+    // Beautify parameters for --help
+    //
+    public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') {
+        Map colors = NfcoreTemplate.logColours(params.monochrome_logs)
         Integer num_hidden = 0
         String output  = ''
         output        += 'Typical pipeline command:\n\n'
         output        += "  ${colors.cyan}${command}${colors.reset}\n\n"
-        Map params_map = params_load(json_schema)
-        Integer max_chars  = params_max_chars(params_map) + 1
+        Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename))
+        Integer max_chars  = paramsMaxChars(params_map) + 1
         Integer desc_indent = max_chars + 14
         Integer dec_linewidth = 160 - desc_indent
         for (group in params_map.keySet()) {
@@ -469,18 +228,17 @@ class NfcoreSchema {
                 output += group_output
             }
         }
-        output += dashed_line(params.monochrome_logs)
         if (num_hidden > 0){
-            output += colors.dim + "\n Hiding $num_hidden params, use --show_hidden_params to show.\n" + colors.reset
-            output += dashed_line(params.monochrome_logs)
+            output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset
         }
+        output += NfcoreTemplate.dashedLine(params.monochrome_logs)
         return output
     }
 
-    /*
-     * Groovy Map summarising parameters/workflow options used by the pipeline
-     */
-    private static LinkedHashMap params_summary_map(workflow, params, json_schema) {
+    //
+    // Groovy Map summarising parameters/workflow options used by the pipeline
+    //
+    public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') {
         // Get a selection of core Nextflow workflow options
         def Map workflow_summary = [:]
         if (workflow.revision) {
@@ -503,7 +261,7 @@ class NfcoreSchema {
         // Get pipeline parameters defined in JSON Schema
         def Map params_summary = [:]
         def blacklist  = ['hostnames']
-        def params_map = params_load(json_schema)
+        def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename))
         for (group in params_map.keySet()) {
             def sub_params = new LinkedHashMap()
             def group_params = params_map.get(group)  // This gets the parameters of that particular group
@@ -546,14 +304,14 @@ class NfcoreSchema {
         return [ 'Core Nextflow options' : workflow_summary ] << params_summary
     }
 
-    /*
-     * Beautify parameters for summary and return as string
-     */
-    private static String params_summary_log(workflow, params, json_schema) {
-        Map colors = log_colours(params.monochrome_logs)
+    //
+    // Beautify parameters for summary and return as string
+    //
+    public static String paramsSummaryLog(workflow, params) {
+        Map colors = NfcoreTemplate.logColours(params.monochrome_logs)
         String output  = ''
-        def params_map = params_summary_map(workflow, params, json_schema)
-        def max_chars  = params_max_chars(params_map)
+        def params_map = paramsSummaryMap(workflow, params)
+        def max_chars  = paramsMaxChars(params_map)
         for (group in params_map.keySet()) {
             def group_params = params_map.get(group)  // This gets the parameters of that particular group
             if (group_params) {
@@ -564,10 +322,196 @@ class NfcoreSchema {
                 output += '\n'
             }
         }
-        output += dashed_line(params.monochrome_logs)
-        output += colors.dim + "\n Only displaying parameters that differ from defaults.\n" + colors.reset
-        output += dashed_line(params.monochrome_logs)
+        output += "!! Only displaying parameters that differ from the pipeline defaults !!\n"
+        output += NfcoreTemplate.dashedLine(params.monochrome_logs)
         return output
     }
 
+    //
+    // Loop over nested exceptions and print the causingException
+    //
+    private static void printExceptions(ex_json, params_json, log) {
+        def causingExceptions = ex_json['causingExceptions']
+        if (causingExceptions.length() == 0) {
+            def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/
+            // Missing required param
+            if (m.matches()) {
+                log.error "* Missing required parameter: --${m[0][1]}"
+            }
+            // Other base-level error
+            else if (ex_json['pointerToViolation'] == '#') {
+                log.error "* ${ex_json['message']}"
+            }
+            // Error with specific param
+            else {
+                def param = ex_json['pointerToViolation'] - ~/^#\//
+                def param_val = params_json[param].toString()
+                log.error "* --${param}: ${ex_json['message']} (${param_val})"
+            }
+        }
+        for (ex in causingExceptions) {
+            printExceptions(ex, params_json, log)
+        }
+    }
+
+    //
+    // Remove an element from a JSONArray
+    //
+    private static JSONArray removeElement(json_array, element) {
+        def list = []
+        int len = json_array.length()
+        for (int i=0;i<len;i++){
+            list.add(json_array.get(i).toString())
+        }
+        list.remove(element)
+        JSONArray jsArray = new JSONArray(list)
+        return jsArray
+    }
+
+    //
+    // Remove ignored parameters
+    //
+    private static JSONObject removeIgnoredParams(raw_schema, params) {
+        // Remove anything that's in params.schema_ignore_params
+        params.schema_ignore_params.split(',').each{ ignore_param ->
+            if(raw_schema.keySet().contains('definitions')){
+                raw_schema.definitions.each { definition ->
+                    for (key in definition.keySet()){
+                        if (definition[key].get("properties").keySet().contains(ignore_param)){
+                            // Remove the param to ignore
+                            definition[key].get("properties").remove(ignore_param)
+                            // If the param was required, change this
+                            if (definition[key].has("required")) {
+                                def cleaned_required = removeElement(definition[key].required, ignore_param)
+                                definition[key].put("required", cleaned_required)
+                            }
+                        }
+                    }
+                }
+            }
+            if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) {
+                raw_schema.get("properties").remove(ignore_param)
+            }
+            if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) {
+                def cleaned_required = removeElement(raw_schema.required, ignore_param)
+                raw_schema.put("required", cleaned_required)
+            }
+        }
+        return raw_schema
+    }
+
+    //
+    // Clean and check parameters relative to Nextflow native classes
+    //
+    private static Map cleanParameters(params) {
+        def new_params = params.getClass().newInstance(params)
+        for (p in params) {
+            // remove anything evaluating to false
+            if (!p['value']) {
+                new_params.remove(p.key)
+            }
+            // Cast MemoryUnit to String
+            if (p['value'].getClass() == nextflow.util.MemoryUnit) {
+                new_params.replace(p.key, p['value'].toString())
+            }
+            // Cast Duration to String
+            if (p['value'].getClass() == nextflow.util.Duration) {
+                new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day"))
+            }
+            // Cast LinkedHashMap to String
+            if (p['value'].getClass() == LinkedHashMap) {
+                new_params.replace(p.key, p['value'].toString())
+            }
+        }
+        return new_params
+    }
+
+    //
+    // This function tries to read a JSON params file
+    //
+    private static LinkedHashMap paramsLoad(String json_schema) {
+        def params_map = new LinkedHashMap()
+        try {
+            params_map = paramsRead(json_schema)
+        } catch (Exception e) {
+            println "Could not read parameters settings from JSON. $e"
+            params_map = new LinkedHashMap()
+        }
+        return params_map
+    }
+
+    //
+    // Method to actually read in JSON file using Groovy.
+    // Group (as Key), values are all parameters
+    //    - Parameter1 as Key, Description as Value
+    //    - Parameter2 as Key, Description as Value
+    //    ....
+    // Group
+    //    -
+    private static LinkedHashMap paramsRead(String json_schema) throws Exception {
+        def json = new File(json_schema).text
+        def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions')
+        def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties')
+        /* Tree looks like this in nf-core schema
+        * definitions <- this is what the first get('definitions') gets us
+                group 1
+                    title
+                    description
+                        properties
+                        parameter 1
+                            type
+                            description
+                        parameter 2
+                            type
+                            description
+                group 2
+                    title
+                    description
+                        properties
+                        parameter 1
+                            type
+                            description
+        * properties <- parameters can also be ungrouped, outside of definitions
+                parameter 1
+                    type
+                    description
+        */
+
+        // Grouped params
+        def params_map = new LinkedHashMap()
+        schema_definitions.each { key, val ->
+            def Map group = schema_definitions."$key".properties // Gets the property object of the group
+            def title = schema_definitions."$key".title
+            def sub_params = new LinkedHashMap()
+            group.each { innerkey, value ->
+                sub_params.put(innerkey, value)
+            }
+            params_map.put(title, sub_params)
+        }
+
+        // Ungrouped params
+        def ungrouped_params = new LinkedHashMap()
+        schema_properties.each { innerkey, value ->
+            ungrouped_params.put(innerkey, value)
+        }
+        params_map.put("Other parameters", ungrouped_params)
+
+        return params_map
+    }
+
+    //
+    // Get maximum number of characters across all parameter names
+    //
+    private static Integer paramsMaxChars(params_map) {
+        Integer max_chars = 0
+        for (group in params_map.keySet()) {
+            def group_params = params_map.get(group)  // This gets the parameters of that particular group
+            for (param in group_params.keySet()) {
+                if (param.size() > max_chars) {
+                    max_chars = param.size()
+                }
+            }
+        }
+        return max_chars
+    }
 }
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
new file mode 100755
index 00000000..b6e689ec
--- /dev/null
+++ b/lib/NfcoreTemplate.groovy
@@ -0,0 +1,266 @@
+//
+// This file holds several functions used within the nf-core pipeline template.
+//
+
+import org.yaml.snakeyaml.Yaml
+
+class NfcoreTemplate {
+
+    //
+    // Check AWS Batch related parameters have been specified correctly
+    //
+    public static void awsBatch(workflow, params) {
+        if (workflow.profile.contains('awsbatch')) {
+            // Check params.awsqueue and params.awsregion have been set if running on AWSBatch
+            assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!"
+            // Check outdir paths to be S3 buckets if running on AWSBatch
+            assert params.outdir.startsWith('s3:')       : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!"
+        }
+    }
+
+    //
+    // Check params.hostnames
+    //
+    public static void hostName(workflow, params, log) {
+        Map colors = logColours(params.monochrome_logs)
+        if (params.hostnames) {
+            def hostname = "hostname".execute().text.trim()
+            params.hostnames.each { prof, hnames ->
+                hnames.each { hname ->
+                    if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
+                        log.info "=${colors.yellow}====================================================${colors.reset}=\n" +
+                            "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" +
+                            "      but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" +
+                            "      ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" +
+                            "=${colors.yellow}====================================================${colors.reset}="
+                    }
+                }
+            }
+        }
+    }
+
+    //
+    // Construct and send completion email
+    //
+    public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) {
+
+        // Set up the e-mail variables
+        def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
+        if (!workflow.success) {
+            subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
+        }
+
+        def summary = [:]
+        for (group in summary_params.keySet()) {
+            summary << summary_params[group]
+        }
+
+        def misc_fields = [:]
+        misc_fields['Date Started']              = workflow.start
+        misc_fields['Date Completed']            = workflow.complete
+        misc_fields['Pipeline script file path'] = workflow.scriptFile
+        misc_fields['Pipeline script hash ID']   = workflow.scriptId
+        if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
+        if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
+        if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
+        misc_fields['Nextflow Version']           = workflow.nextflow.version
+        misc_fields['Nextflow Build']             = workflow.nextflow.build
+        misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
+
+        def email_fields = [:]
+        email_fields['version']      = workflow.manifest.version
+        email_fields['runName']      = workflow.runName
+        email_fields['success']      = workflow.success
+        email_fields['dateComplete'] = workflow.complete
+        email_fields['duration']     = workflow.duration
+        email_fields['exitStatus']   = workflow.exitStatus
+        email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+        email_fields['errorReport']  = (workflow.errorReport ?: 'None')
+        email_fields['commandLine']  = workflow.commandLine
+        email_fields['projectDir']   = workflow.projectDir
+        email_fields['summary']      = summary << misc_fields
+
+        // On success try attach the multiqc report
+        def mqc_report = null
+        try {
+            if (workflow.success) {
+                mqc_report = multiqc_report.getVal()
+                if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
+                    if (mqc_report.size() > 1) {
+                        log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
+                    }
+                    mqc_report = mqc_report[0]
+                }
+            }
+        } catch (all) {
+            if (multiqc_report) {
+                log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
+            }
+        }
+
+        // Check if we are only sending emails on failure
+        def email_address = params.email
+        if (!params.email && params.email_on_fail && !workflow.success) {
+            email_address = params.email_on_fail
+        }
+
+        // Render the TXT template
+        def engine       = new groovy.text.GStringTemplateEngine()
+        def tf           = new File("$projectDir/assets/email_template.txt")
+        def txt_template = engine.createTemplate(tf).make(email_fields)
+        def email_txt    = txt_template.toString()
+
+        // Render the HTML template
+        def hf            = new File("$projectDir/assets/email_template.html")
+        def html_template = engine.createTemplate(hf).make(email_fields)
+        def email_html    = html_template.toString()
+
+        // Render the sendmail template
+        def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit
+        def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
+        def sf                     = new File("$projectDir/assets/sendmail_template.txt")
+        def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
+        def sendmail_html          = sendmail_template.toString()
+
+        // Send the HTML e-mail
+        Map colors = logColours(params.monochrome_logs)
+        if (email_address) {
+            try {
+                if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
+                // Try to send HTML e-mail using sendmail
+                [ 'sendmail', '-t' ].execute() << sendmail_html
+                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
+            } catch (all) {
+                // Catch failures and try with plaintext
+                def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
+                if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
+                    mail_cmd += [ '-A', mqc_report ]
+                }
+                mail_cmd.execute() << email_html
+                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
+            }
+        }
+
+        // Write summary e-mail HTML to a file
+        def output_d = new File("${params.outdir}/pipeline_info/")
+        if (!output_d.exists()) {
+            output_d.mkdirs()
+        }
+        def output_hf = new File(output_d, "pipeline_report.html")
+        output_hf.withWriter { w -> w << email_html }
+        def output_tf = new File(output_d, "pipeline_report.txt")
+        output_tf.withWriter { w -> w << email_txt }
+    }
+
+    //
+    // Print pipeline summary on completion
+    //
+    public static void summary(workflow, params, log) {
+        Map colors = logColours(params.monochrome_logs)
+        if (workflow.success) {
+            if (workflow.stats.ignoredCount == 0) {
+                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
+            } else {
+                log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
+            }
+        } else {
+            hostName(workflow, params, log)
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
+        }
+    }
+
+    //
+    // ANSII Colours used for terminal logging
+    //
+    public static Map logColours(Boolean monochrome_logs) {
+        Map colorcodes = [:]
+
+        // Reset / Meta
+        colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
+        colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
+        colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
+        colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
+        colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
+        colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
+        colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
+
+        // Regular Colors
+        colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
+        colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
+        colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
+        colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
+        colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
+        colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
+        colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
+        colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
+
+        // Bold
+        colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
+        colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
+        colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
+        colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
+        colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
+        colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
+        colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
+        colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
+
+        // Underline
+        colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
+        colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
+        colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
+        colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
+        colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
+        colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
+        colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
+        colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
+
+        // High Intensity
+        colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
+        colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
+        colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
+        colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
+        colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
+        colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
+        colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
+        colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
+
+        // Bold High Intensity
+        colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
+        colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
+        colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
+        colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
+        colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
+        colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
+        colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
+        colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
+
+        return colorcodes
+    }
+
+    //
+    // Does what is says on the tin
+    //
+    public static String dashedLine(monochrome_logs) {
+        Map colors = logColours(monochrome_logs)
+        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
+    }
+
+    //
+    // nf-core logo
+    //
+    public static String logo(workflow, monochrome_logs) {
+        Map colors = logColours(monochrome_logs)
+        String.format(
+            """\n
+            ${dashedLine(monochrome_logs)}
+                                                    ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
+            ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
+            ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
+            ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
+                                                    ${colors.green}`._,._,\'${colors.reset}
+            ${colors.purple}  ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset}
+            ${dashedLine(monochrome_logs)}
+            """.stripIndent()
+        )
+    }
+}
diff --git a/lib/Utils.groovy b/lib/Utils.groovy
new file mode 100755
index 00000000..18173e98
--- /dev/null
+++ b/lib/Utils.groovy
@@ -0,0 +1,47 @@
+//
+// This file holds several Groovy functions that could be useful for any Nextflow pipeline
+//
+
+import org.yaml.snakeyaml.Yaml
+
+class Utils {
+
+    //
+    // When running with -profile conda, warn if channels have not been set-up appropriately
+    //
+    public static void checkCondaChannels(log) {
+        Yaml parser = new Yaml()
+        def channels = []
+        try {
+            def config = parser.load("conda config --show channels".execute().text)
+            channels = config.channels
+        } catch(NullPointerException | IOException e) {
+            log.warn "Could not verify conda channel configuration."
+            return
+        }
+
+        // Check that all channels are present
+        def required_channels = ['conda-forge', 'bioconda', 'defaults']
+        def conda_check_failed = !required_channels.every { ch -> ch in channels }
+
+        // Check that they are in the right order
+        conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda'))
+        conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults'))
+
+        if (conda_check_failed) {
+            log.warn "=============================================================================\n" +
+                "  There is a problem with your Conda configuration!\n\n" +
+                "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
+                "  Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" +
+                "  NB: The order of the channels matters!\n" +
+                "==================================================================================="
+        }
+    }
+
+    //
+    // Join module args with appropriate spacing
+    //
+    public static String joinModuleArgs(args_list) {
+        return ' ' + args_list.join(' ')
+    }
+}
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
new file mode 100755
index 00000000..4caed266
--- /dev/null
+++ b/lib/WorkflowMain.groovy
@@ -0,0 +1,94 @@
+//
+// This file holds several functions specific to the main.nf workflow in the nf-core/rnafusion pipeline
+//
+
+class WorkflowMain {
+
+    //
+    // Citation string for pipeline
+    //
+    public static String citation(workflow) {
+        return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
+            // TODO nf-core: Add Zenodo DOI for pipeline after first release
+            //"* The pipeline\n" +
+            //"  https://doi.org/10.5281/zenodo.XXXXXXX\n\n" +
+            "* The nf-core framework\n" +
+            "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
+            "* Software dependencies\n" +
+            "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
+    }
+
+    //
+    // Print help to screen if required
+    //
+    public static String help(workflow, params, log) {
+        def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker"
+        def help_string = ''
+        help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs)
+        help_string += NfcoreSchema.paramsHelp(workflow, params, command)
+        help_string += '\n' + citation(workflow) + '\n'
+        help_string += NfcoreTemplate.dashedLine(params.monochrome_logs)
+        return help_string
+    }
+
+    //
+    // Print parameter summary log to screen
+    //
+    public static String paramsSummaryLog(workflow, params, log) {
+        def summary_log = ''
+        summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs)
+        summary_log += NfcoreSchema.paramsSummaryLog(workflow, params)
+        summary_log += '\n' + citation(workflow) + '\n'
+        summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs)
+        return summary_log
+    }
+
+    //
+    // Validate parameters and print summary to screen
+    //
+    public static void initialise(workflow, params, log) {
+        // Print help to screen if required
+        if (params.help) {
+            log.info help(workflow, params, log)
+            System.exit(0)
+        }
+
+        // Validate workflow parameters via the JSON schema
+        if (params.validate_params) {
+            NfcoreSchema.validateParameters(workflow, params, log)
+        }
+
+        // Print parameter summary log to screen
+        log.info paramsSummaryLog(workflow, params, log)
+
+        // Check that conda channels are set-up correctly
+        if (params.enable_conda) {
+            Utils.checkCondaChannels(log)
+        }
+
+        // Check AWS batch settings
+        NfcoreTemplate.awsBatch(workflow, params)
+
+        // Check the hostnames against configured profiles
+        NfcoreTemplate.hostName(workflow, params, log)
+
+        // Check input has been provided
+        if (!params.input) {
+            log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'"
+            System.exit(1)
+        }
+    }
+
+    //
+    // Get attribute from genome config file e.g. fasta
+    //
+    public static String getGenomeAttribute(params, attribute) {
+        def val = ''
+        if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) {
+            if (params.genomes[ params.genome ].containsKey(attribute)) {
+                val = params.genomes[ params.genome ][ attribute ]
+            }
+        }
+        return val
+    }
+}
diff --git a/lib/WorkflowRnafusion.groovy b/lib/WorkflowRnafusion.groovy
new file mode 100755
index 00000000..e9d53d8a
--- /dev/null
+++ b/lib/WorkflowRnafusion.groovy
@@ -0,0 +1,59 @@
+//
+// This file holds several functions specific to the workflow/rnafusion.nf in the nf-core/rnafusion pipeline
+//
+
+class WorkflowRnafusion {
+
+    //
+    // Check and validate parameters
+    //
+    public static void initialise(params, log) {
+        genomeExistsError(params, log)
+
+        if (!params.fasta) {
+            log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
+            System.exit(1)
+        }
+    }
+
+    //
+    // Get workflow summary for MultiQC
+    //
+    public static String paramsSummaryMultiqc(workflow, summary) {
+        String summary_section = ''
+        for (group in summary.keySet()) {
+            def group_params = summary.get(group)  // This gets the parameters of that particular group
+            if (group_params) {
+                summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
+                summary_section += "    <dl class=\"dl-horizontal\">\n"
+                for (param in group_params.keySet()) {
+                    summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
+                }
+                summary_section += "    </dl>\n"
+            }
+        }
+
+        String yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n"
+        yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
+        yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
+        yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
+        yaml_file_text        += "plot_type: 'html'\n"
+        yaml_file_text        += "data: |\n"
+        yaml_file_text        += "${summary_section}"
+        return yaml_file_text
+    }
+
+    //
+    // Exit pipeline if incorrect --genome key provided
+    //
+    private static void genomeExistsError(params, log) {
+        if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
+            log.error "=============================================================================\n" +
+                "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
+                "  Currently, the available genome keys are:\n" +
+                "  ${params.genomes.keySet().join(", ")}\n" +
+                "==================================================================================="
+            System.exit(1)
+        }
+    }
+}
diff --git a/main.nf b/main.nf
index 38c5404c..2b33ffe8 100644
--- a/main.nf
+++ b/main.nf
@@ -1,390 +1,63 @@
 #!/usr/bin/env nextflow
 /*
 ========================================================================================
-                         nf-core/rnafusion
+    nf-core/rnafusion
 ========================================================================================
- nf-core/rnafusion Analysis Pipeline.
- #### Homepage / Documentation
- https://github.com/nf-core/rnafusion
+    Github : https://github.com/nf-core/rnafusion
+    Website: https://nf-co.re/rnafusion
+    Slack  : https://nfcore.slack.com/channels/rnafusion
 ----------------------------------------------------------------------------------------
 */
 
-log.info Headers.nf_core(workflow, params.monochrome_logs)
-
-////////////////////////////////////////////////////
-/* --               PRINT HELP                 -- */
-////////////////////////////////////////////////////+
-def json_schema = "$projectDir/nextflow_schema.json"
-if (params.help) {
-    def command = "nextflow run nf-core/rnafusion --input '*_R{1,2}.fastq.gz' -profile docker"
-    log.info NfcoreSchema.params_help(workflow, params, json_schema, command)
-    exit 0
-}
-
-////////////////////////////////////////////////////
-/* --         VALIDATE PARAMETERS              -- */
-////////////////////////////////////////////////////+
-if (params.validate_params) {
-    NfcoreSchema.validateParameters(params, json_schema, log)
-}
-
-////////////////////////////////////////////////////
-/* --     Collect configuration parameters     -- */
-////////////////////////////////////////////////////
-
-// Check if genome exists in the config file
-if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
-    exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(', ')}"
-}
-
-// TODO nf-core: Add any reference files that are needed
-// Configurable reference genomes
-//
-// NOTE - THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY
-// If you want to use the channel below in a process, define the following:
-//   input:
-//   file fasta from ch_fasta
-//
-params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false
-if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) }
-
-// Check AWS batch settings
-if (workflow.profile.contains('awsbatch')) {
-    // AWSBatch sanity checking
-    if (!params.awsqueue || !params.awsregion) exit 1, 'Specify correct --awsqueue and --awsregion parameters on AWSBatch!'
-    // Check outdir paths to be S3 buckets if running on AWSBatch
-    // related: https://github.com/nextflow-io/nextflow/issues/813
-    if (!params.outdir.startsWith('s3:')) exit 1, 'Outdir not on S3 - specify S3 Bucket to run on AWSBatch!'
-    // Prevent trace files to be stored on S3 since S3 does not support rolling files.
-    if (params.tracedir.startsWith('s3:')) exit 1, 'Specify a local tracedir or run without trace! S3 cannot be used for tracefiles.'
-}
-
-// Stage config files
-ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true)
-ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty()
-ch_output_docs = file("$projectDir/docs/output.md", checkIfExists: true)
-ch_output_docs_images = file("$projectDir/docs/images/", checkIfExists: true)
+nextflow.enable.dsl = 2
 
 /*
- * Create a channel for input read files
- */
-if (params.input_paths) {
-    if (params.single_end) {
-        Channel
-            .from(params.input_paths)
-            .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] }
-            .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' }
-            .into { ch_read_files_fastqc; ch_read_files_trimming }
-    } else {
-        Channel
-            .from(params.input_paths)
-            .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] }
-            .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' }
-            .into { ch_read_files_fastqc; ch_read_files_trimming }
-    }
-} else {
-    Channel
-        .fromFilePairs(params.input, size: params.single_end ? 1 : 2)
-        .ifEmpty { exit 1, "Cannot find any reads matching: ${params.input}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." }
-        .into { ch_read_files_fastqc; ch_read_files_trimming }
-}
-
-////////////////////////////////////////////////////
-/* --         PRINT PARAMETER SUMMARY          -- */
-////////////////////////////////////////////////////
-log.info NfcoreSchema.params_summary_log(workflow, params, json_schema)
-
-// Header log info
-def summary = [:]
-if (workflow.revision) summary['Pipeline Release'] = workflow.revision
-summary['Run Name']         = workflow.runName
-// TODO nf-core: Report custom parameters here
-summary['Input']            = params.input
-summary['Fasta Ref']        = params.fasta
-summary['Data Type']        = params.single_end ? 'Single-End' : 'Paired-End'
-summary['Max Resources']    = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job"
-if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container"
-summary['Output dir']       = params.outdir
-summary['Launch dir']       = workflow.launchDir
-summary['Working dir']      = workflow.workDir
-summary['Script dir']       = workflow.projectDir
-summary['User']             = workflow.userName
-if (workflow.profile.contains('awsbatch')) {
-    summary['AWS Region']   = params.awsregion
-    summary['AWS Queue']    = params.awsqueue
-    summary['AWS CLI']      = params.awscli
-}
-summary['Config Profile'] = workflow.profile
-if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description
-if (params.config_profile_contact)     summary['Config Profile Contact']     = params.config_profile_contact
-if (params.config_profile_url)         summary['Config Profile URL']         = params.config_profile_url
-summary['Config Files'] = workflow.configFiles.join(', ')
-if (params.email || params.email_on_fail) {
-    summary['E-mail Address']    = params.email
-    summary['E-mail on failure'] = params.email_on_fail
-    summary['MultiQC maxsize']   = params.max_multiqc_email_size
-}
-
-// Check the hostnames against configured profiles
-checkHostname()
-
-Channel.from(summary.collect{ [it.key, it.value] })
-    .map { k,v -> "<dt>$k</dt><dd><samp>${v ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>" }
-    .reduce { a, b -> return [a, b].join("\n            ") }
-    .map { x -> """
-    id: 'nf-core-rnafusion-summary'
-    description: " - this information is collected when the pipeline is started."
-    section_name: 'nf-core/rnafusion Workflow Summary'
-    section_href: 'https://github.com/nf-core/rnafusion'
-    plot_type: 'html'
-    data: |
-        <dl class=\"dl-horizontal\">
-            $x
-        </dl>
-    """.stripIndent() }
-    .set { ch_workflow_summary }
-
-/*
- * Parse software version numbers
- */
-process get_software_versions {
-    publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode,
-        saveAs: { filename ->
-                      if (filename.indexOf('.csv') > 0) filename
-                      else null
-        }
-
-    output:
-    file 'software_versions_mqc.yaml' into ch_software_versions_yaml
-    file 'software_versions.csv'
+========================================================================================
+    GENOME PARAMETER VALUES
+========================================================================================
+*/
 
-    script:
-    // TODO nf-core: Get all tools to print their version number here
-    """
-    echo $workflow.manifest.version > v_pipeline.txt
-    echo $workflow.nextflow.version > v_nextflow.txt
-    fastqc --version > v_fastqc.txt
-    multiqc --version > v_multiqc.txt
-    scrape_software_versions.py &> software_versions_mqc.yaml
-    """
-}
+params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
 
 /*
- * STEP 1 - FastQC
- */
-process fastqc {
-    tag "$name"
-    label 'process_medium'
-    publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode,
-        saveAs: { filename ->
-                      filename.indexOf('.zip') > 0 ? "zips/$filename" : "$filename"
-        }
-
-    input:
-    set val(name), file(reads) from ch_read_files_fastqc
-
-    output:
-    file '*_fastqc.{zip,html}' into ch_fastqc_results
+========================================================================================
+    VALIDATE & PRINT PARAMETER SUMMARY
+========================================================================================
+*/
 
-    script:
-    """
-    fastqc --quiet --threads $task.cpus $reads
-    """
-}
+WorkflowMain.initialise(workflow, params, log)
 
 /*
- * STEP 2 - MultiQC
- */
-process multiqc {
-    publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode
-
-    input:
-    file (multiqc_config) from ch_multiqc_config
-    file (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([])
-    // TODO nf-core: Add in log files from your new processes for MultiQC to find!
-    file ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([])
-    file ('software_versions/*') from ch_software_versions_yaml.collect()
-    file workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml")
+========================================================================================
+    NAMED WORKFLOW FOR PIPELINE
+========================================================================================
+*/
 
-    output:
-    file "*multiqc_report.html" into ch_multiqc_report
-    file "*_data"
-    file "multiqc_plots"
+include { RNAFUSION } from './workflows/rnafusion'
 
-    script:
-    rtitle = ''
-    rfilename = ''
-    if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) {
-        rtitle = "--title \"${workflow.runName}\""
-        rfilename = "--filename " + workflow.runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report"
-    }
-    custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : ''
-    // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time
-    """
-    multiqc -f $rtitle $rfilename $custom_config_file .
-    """
+//
+// WORKFLOW: Run main nf-core/rnafusion analysis pipeline
+//
+workflow NFCORE_RNAFUSION {
+    RNAFUSION ()
 }
 
 /*
- * STEP 3 - Output Description HTML
- */
-process output_documentation {
-    publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode
-
-    input:
-    file output_docs from ch_output_docs
-    file images from ch_output_docs_images
-
-    output:
-    file 'results_description.html'
+========================================================================================
+    RUN ALL WORKFLOWS
+========================================================================================
+*/
 
-    script:
-    """
-    markdown_to_html.py $output_docs -o results_description.html
-    """
+//
+// WORKFLOW: Execute a single named workflow for the pipeline
+// See: https://github.com/nf-core/rnaseq/issues/619
+//
+workflow {
+    NFCORE_RNAFUSION ()
 }
 
 /*
- * Completion e-mail notification
- */
-workflow.onComplete {
-
-    // Set up the e-mail variables
-    def subject = "[nf-core/rnafusion] Successful: $workflow.runName"
-    if (!workflow.success) {
-        subject = "[nf-core/rnafusion] FAILED: $workflow.runName"
-    }
-    def email_fields = [:]
-    email_fields['version'] = workflow.manifest.version
-    email_fields['runName'] = workflow.runName
-    email_fields['success'] = workflow.success
-    email_fields['dateComplete'] = workflow.complete
-    email_fields['duration'] = workflow.duration
-    email_fields['exitStatus'] = workflow.exitStatus
-    email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
-    email_fields['errorReport'] = (workflow.errorReport ?: 'None')
-    email_fields['commandLine'] = workflow.commandLine
-    email_fields['projectDir'] = workflow.projectDir
-    email_fields['summary'] = summary
-    email_fields['summary']['Date Started'] = workflow.start
-    email_fields['summary']['Date Completed'] = workflow.complete
-    email_fields['summary']['Pipeline script file path'] = workflow.scriptFile
-    email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId
-    if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository
-    if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId
-    if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision
-    email_fields['summary']['Nextflow Version'] = workflow.nextflow.version
-    email_fields['summary']['Nextflow Build'] = workflow.nextflow.build
-    email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
-
-    // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size)
-    // On success try attach the multiqc report
-    def mqc_report = null
-    try {
-        if (workflow.success) {
-            mqc_report = ch_multiqc_report.getVal()
-            if (mqc_report.getClass() == ArrayList) {
-                log.warn "[nf-core/rnafusion] Found multiple reports from process 'multiqc', will use only one"
-                mqc_report = mqc_report[0]
-            }
-        }
-    } catch (all) {
-        log.warn "[nf-core/rnafusion] Could not attach MultiQC report to summary email"
-    }
-
-    // Check if we are only sending emails on failure
-    email_address = params.email
-    if (!params.email && params.email_on_fail && !workflow.success) {
-        email_address = params.email_on_fail
-    }
-
-    // Render the TXT template
-    def engine = new groovy.text.GStringTemplateEngine()
-    def tf = new File("$projectDir/assets/email_template.txt")
-    def txt_template = engine.createTemplate(tf).make(email_fields)
-    def email_txt = txt_template.toString()
-
-    // Render the HTML template
-    def hf = new File("$projectDir/assets/email_template.html")
-    def html_template = engine.createTemplate(hf).make(email_fields)
-    def email_html = html_template.toString()
-
-    // Render the sendmail template
-    def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ]
-    def sf = new File("$projectDir/assets/sendmail_template.txt")
-    def sendmail_template = engine.createTemplate(sf).make(smail_fields)
-    def sendmail_html = sendmail_template.toString()
-
-    // Send the HTML e-mail
-    if (email_address) {
-        try {
-            if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
-            // Try to send HTML e-mail using sendmail
-            [ 'sendmail', '-t' ].execute() << sendmail_html
-            log.info "[nf-core/rnafusion] Sent summary e-mail to $email_address (sendmail)"
-        } catch (all) {
-            // Catch failures and try with plaintext
-            def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
-            if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) {
-              mail_cmd += [ '-A', mqc_report ]
-            }
-            mail_cmd.execute() << email_html
-            log.info "[nf-core/rnafusion] Sent summary e-mail to $email_address (mail)"
-        }
-    }
-
-    // Write summary e-mail HTML to a file
-    def output_d = new File("${params.outdir}/pipeline_info/")
-    if (!output_d.exists()) {
-        output_d.mkdirs()
-    }
-    def output_hf = new File(output_d, "pipeline_report.html")
-    output_hf.withWriter { w -> w << email_html }
-    def output_tf = new File(output_d, "pipeline_report.txt")
-    output_tf.withWriter { w -> w << email_txt }
-
-    c_green = params.monochrome_logs ? '' : "\033[0;32m";
-    c_purple = params.monochrome_logs ? '' : "\033[0;35m";
-    c_red = params.monochrome_logs ? '' : "\033[0;31m";
-    c_reset = params.monochrome_logs ? '' : "\033[0m";
-
-    if (workflow.stats.ignoredCount > 0 && workflow.success) {
-        log.info "-${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}-"
-        log.info "-${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${c_reset}-"
-        log.info "-${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${c_reset}-"
-    }
-
-    if (workflow.success) {
-        log.info "-${c_purple}[nf-core/rnafusion]${c_green} Pipeline completed successfully${c_reset}-"
-    } else {
-        checkHostname()
-        log.info "-${c_purple}[nf-core/rnafusion]${c_red} Pipeline completed with errors${c_reset}-"
-    }
-
-}
-
-workflow.onError {
-    // Print unexpected parameters - easiest is to just rerun validation
-    NfcoreSchema.validateParameters(params, json_schema, log)
-}
-
-def checkHostname() {
-    def c_reset = params.monochrome_logs ? '' : "\033[0m"
-    def c_white = params.monochrome_logs ? '' : "\033[0;37m"
-    def c_red = params.monochrome_logs ? '' : "\033[1;91m"
-    def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m"
-    if (params.hostnames) {
-        def hostname = 'hostname'.execute().text.trim()
-        params.hostnames.each { prof, hnames ->
-            hnames.each { hname ->
-                if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                    log.error "${c_red}====================================================${c_reset}\n" +
-                            "  ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" +
-                            "  but your machine hostname is ${c_white}'$hostname'${c_reset}\n" +
-                            "  ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" +
-                            "${c_red}====================================================${c_reset}\n"
-                }
-            }
-        }
-    }
-}
+========================================================================================
+    THE END
+========================================================================================
+*/
diff --git a/modules.json b/modules.json
new file mode 100644
index 00000000..5e4e806f
--- /dev/null
+++ b/modules.json
@@ -0,0 +1,14 @@
+{
+    "name": "nf-core/rnafusion",
+    "homePage": "https://github.com/nf-core/rnafusion",
+    "repos": {
+        "nf-core/modules": {
+            "fastqc": {
+                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+            },
+            "multiqc": {
+                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+            }
+        }
+    }
+}
diff --git a/modules/local/functions.nf b/modules/local/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/local/get_software_versions.nf b/modules/local/get_software_versions.nf
new file mode 100644
index 00000000..2568f170
--- /dev/null
+++ b/modules/local/get_software_versions.nf
@@ -0,0 +1,33 @@
+// Import generic module functions
+include { saveFiles } from './functions'
+
+params.options = [:]
+
+process GET_SOFTWARE_VERSIONS {
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/python:3.8.3"
+    } else {
+        container "quay.io/biocontainers/python:3.8.3"
+    }
+
+    cache false
+
+    input:
+    path versions
+
+    output:
+    path "software_versions.tsv"     , emit: tsv
+    path 'software_versions_mqc.yaml', emit: yaml
+
+    script: // This script is bundled with the pipeline, in nf-core/rnafusion/bin/
+    """
+    echo $workflow.manifest.version > pipeline.version.txt
+    echo $workflow.nextflow.version > nextflow.version.txt
+    scrape_software_versions.py &> software_versions_mqc.yaml
+    """
+}
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
new file mode 100644
index 00000000..5cff46fe
--- /dev/null
+++ b/modules/local/samplesheet_check.nf
@@ -0,0 +1,31 @@
+// Import generic module functions
+include { saveFiles } from './functions'
+
+params.options = [:]
+
+process SAMPLESHEET_CHECK {
+    tag "$samplesheet"
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/python:3.8.3"
+    } else {
+        container "quay.io/biocontainers/python:3.8.3"
+    }
+
+    input:
+    path samplesheet
+
+    output:
+    path '*.csv'
+
+    script: // This script is bundled with the pipeline, in nf-core/rnafusion/bin/
+    """
+    check_samplesheet.py \\
+        $samplesheet \\
+        samplesheet.valid.csv
+    """
+}
diff --git a/modules/nf-core/modules/fastqc/functions.nf b/modules/nf-core/modules/fastqc/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/fastqc/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
new file mode 100644
index 00000000..39c327b2
--- /dev/null
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -0,0 +1,47 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process FASTQC {
+    tag "$meta.id"
+    label 'process_medium'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+
+    conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0"
+    } else {
+        container "quay.io/biocontainers/fastqc:0.11.9--0"
+    }
+
+    input:
+    tuple val(meta), path(reads)
+
+    output:
+    tuple val(meta), path("*.html"), emit: html
+    tuple val(meta), path("*.zip") , emit: zip
+    path  "*.version.txt"          , emit: version
+
+    script:
+    // Add soft-links to original FastQs for consistent naming in pipeline
+    def software = getSoftwareName(task.process)
+    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    if (meta.single_end) {
+        """
+        [ ! -f  ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
+        fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz
+        fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
+        """
+    } else {
+        """
+        [ ! -f  ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
+        [ ! -f  ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
+        fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
+        fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
+        """
+    }
+}
diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml
new file mode 100644
index 00000000..8eb9953d
--- /dev/null
+++ b/modules/nf-core/modules/fastqc/meta.yml
@@ -0,0 +1,51 @@
+name: fastqc
+description: Run FastQC on sequenced reads
+keywords:
+    - quality control
+    - qc
+    - adapters
+    - fastq
+tools:
+    - fastqc:
+        description: |
+            FastQC gives general quality metrics about your reads.
+            It provides information about the quality score distribution
+            across your reads, the per base sequence content (%A/C/G/T).
+            You get information about adapter contamination and other
+            overrepresented sequences.
+        homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
+        documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
+input:
+    - meta:
+        type: map
+        description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+            List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+            respectively.
+output:
+    - meta:
+        type: map
+        description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+    - html:
+        type: file
+        description: FastQC report
+        pattern: "*_{fastqc.html}"
+    - zip:
+        type: file
+        description: FastQC report archive
+        pattern: "*_{fastqc.zip}"
+    - version:
+        type: file
+        description: File containing software version
+        pattern: "*.{version.txt}"
+authors:
+    - "@drpatelh"
+    - "@grst"
+    - "@ewels"
+    - "@FelixKrueger"
diff --git a/modules/nf-core/modules/multiqc/functions.nf b/modules/nf-core/modules/multiqc/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/multiqc/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
new file mode 100644
index 00000000..da780800
--- /dev/null
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -0,0 +1,35 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process MULTIQC {
+    label 'process_medium'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "bioconda::multiqc=1.10.1" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/multiqc:1.10.1--py_0"
+    } else {
+        container "quay.io/biocontainers/multiqc:1.10.1--py_0"
+    }
+
+    input:
+    path multiqc_files
+
+    output:
+    path "*multiqc_report.html", emit: report
+    path "*_data"              , emit: data
+    path "*_plots"             , optional:true, emit: plots
+    path "*.version.txt"       , emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+    """
+    multiqc -f $options.args .
+    multiqc --version | sed -e "s/multiqc, version //g" > ${software}.version.txt
+    """
+}
diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml
new file mode 100644
index 00000000..532a8bb1
--- /dev/null
+++ b/modules/nf-core/modules/multiqc/meta.yml
@@ -0,0 +1,39 @@
+name: MultiQC
+description: Aggregate results from bioinformatics analyses across many samples into a single report
+keywords:
+    - QC
+    - bioinformatics tools
+    - Beautiful stand-alone HTML report
+tools:
+    - multiqc:
+        description: |
+            MultiQC searches a given directory for analysis logs and compiles a HTML report.
+            It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
+        homepage: https://multiqc.info/
+        documentation: https://multiqc.info/docs/
+input:
+    - multiqc_files:
+        type: file
+        description: |
+            List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+output:
+    - report:
+        type: file
+        description: MultiQC report file
+        pattern: "multiqc_report.html"
+    - data:
+        type: dir
+        description: MultiQC data dir
+        pattern: "multiqc_data"
+    - plots:
+        type: file
+        description: Plots created by MultiQC
+        pattern: "*_data"
+    - version:
+        type: file
+        description: File containing software version
+        pattern: "*.{version.txt}"
+authors:
+    - "@abhi18av"
+    - "@bunop"
+    - "@drpatelh"
diff --git a/nextflow.config b/nextflow.config
index 603a4b94..118c2479 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -1,130 +1,136 @@
 /*
- * -------------------------------------------------
- *  nf-core/rnafusion Nextflow config file
- * -------------------------------------------------
- * Default config options for all environments.
- */
+========================================================================================
+    nf-core/rnafusion Nextflow config file
+========================================================================================
+    Default config options for all compute environments
+----------------------------------------------------------------------------------------
+*/
 
 // Global default params, used in configs
 params {
 
-  // Workflow flags
-  // TODO nf-core: Specify your pipeline's command line flags
-  genome = false
-  input = null
-  input_paths = null
-  single_end = false
-  outdir = './results'
-  publish_dir_mode = 'copy'
-
-  // Boilerplate options
-  multiqc_config = false
-  email = false
-  email_on_fail = false
-  max_multiqc_email_size = 25.MB
-  plaintext_email = false
-  monochrome_logs = false
-  help = false
-  igenomes_base = 's3://ngi-igenomes/igenomes'
-  tracedir = "${params.outdir}/pipeline_info"
-  igenomes_ignore = false
-  custom_config_version = 'master'
-  custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
-  hostnames = false
-  config_profile_name = null
-  config_profile_description = false
-  config_profile_contact = false
-  config_profile_url = false
-  validate_params = true
-  show_hidden_params = false
-  schema_ignore_params = 'genomes,input_paths'
-
-  // Defaults only, expecting to be overwritten
-  max_memory = 128.GB
-  max_cpus = 16
-  max_time = 240.h
+    // TODO nf-core: Specify your pipeline's command line flags
+    // Input options
+    input                      = null
 
-}
+    // References
+    genome                     = null
+    igenomes_base              = 's3://ngi-igenomes/igenomes'
+    igenomes_ignore            = false
+
+    // MultiQC options
+    multiqc_config             = null
+    multiqc_title              = null
+    max_multiqc_email_size     = '25.MB'
+
+    // Boilerplate options
+    outdir                     = './results'
+    tracedir                   = "${params.outdir}/pipeline_info"
+    publish_dir_mode           = 'copy'
+    email                      = null
+    email_on_fail              = null
+    plaintext_email            = false
+    monochrome_logs            = false
+    help                       = false
+    validate_params            = true
+    show_hidden_params         = false
+    schema_ignore_params       = 'genomes,modules'
+    enable_conda               = false
+    singularity_pull_docker_container = false
 
-// Container slug. Stable releases should specify release tag!
-// Developmental code should specify :dev
-process.container = 'nfcore/rnafusion:dev'
+    // Config options
+    custom_config_version      = 'master'
+    custom_config_base         = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
+    hostnames                  = [:]
+    config_profile_description = null
+    config_profile_contact     = null
+    config_profile_url         = null
+    config_profile_name        = null
+
+    // Max resource options
+    // Defaults only, expecting to be overwritten
+    max_memory                 = '128.GB'
+    max_cpus                   = 16
+    max_time                   = '240.h'
+
+}
 
 // Load base.config by default for all pipelines
 includeConfig 'conf/base.config'
 
+// Load modules.config for DSL2 module specific options
+includeConfig 'conf/modules.config'
+
 // Load nf-core custom profiles from different Institutions
 try {
-  includeConfig "${params.custom_config_base}/nfcore_custom.config"
+    includeConfig "${params.custom_config_base}/nfcore_custom.config"
 } catch (Exception e) {
-  System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
-}
-
-profiles {
-  conda {
-    docker.enabled = false
-    singularity.enabled = false
-    podman.enabled = false
-    shifter.enabled = false
-    charliecloud.enabled = false
-    process.conda = "$projectDir/environment.yml"
-  }
-  debug { process.beforeScript = 'echo $HOSTNAME' }
-  docker {
-    docker.enabled = true
-    singularity.enabled = false
-    podman.enabled = false
-    shifter.enabled = false
-    charliecloud.enabled = false
-    // Avoid this error:
-    //   WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap.
-    // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351
-    // once this is established and works well, nextflow might implement this behavior as new default.
-    docker.runOptions = '-u \$(id -u):\$(id -g)'
-  }
-  singularity {
-    docker.enabled = false
-    singularity.enabled = true
-    podman.enabled = false
-    shifter.enabled = false
-    charliecloud.enabled = false
-    singularity.autoMounts = true
-  }
-  podman {
-    singularity.enabled = false
-    docker.enabled = false
-    podman.enabled = true
-    shifter.enabled = false
-    charliecloud.enabled = false
-  }
-  shifter {
-    singularity.enabled = false
-    docker.enabled = false
-    podman.enabled = false
-    shifter.enabled = true
-    charliecloud.enabled = false
-  }
-  charliecloud {
-    singularity.enabled = false
-    docker.enabled = false
-    podman.enabled = false
-    shifter.enabled = false
-    charliecloud.enabled = true
-  }
-  test { includeConfig 'conf/test.config' }
-  test_full { includeConfig 'conf/test_full.config' }
+    System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
 }
 
 // Load igenomes.config if required
 if (!params.igenomes_ignore) {
-  includeConfig 'conf/igenomes.config'
+    includeConfig 'conf/igenomes.config'
+} else {
+    params.genomes = [:]
+}
+
+profiles {
+    debug { process.beforeScript = 'echo $HOSTNAME' }
+    conda {
+        params.enable_conda    = true
+        docker.enabled         = false
+        singularity.enabled    = false
+        podman.enabled         = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
+    }
+    docker {
+        docker.enabled         = true
+        docker.userEmulation   = true
+        singularity.enabled    = false
+        podman.enabled         = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
+    }
+    singularity {
+        singularity.enabled    = true
+        singularity.autoMounts = true
+        docker.enabled         = false
+        podman.enabled         = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
+    }
+    podman {
+        podman.enabled         = true
+        docker.enabled         = false
+        singularity.enabled    = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
+    }
+    shifter {
+        shifter.enabled        = true
+        docker.enabled         = false
+        singularity.enabled    = false
+        podman.enabled         = false
+        charliecloud.enabled   = false
+    }
+    charliecloud {
+        charliecloud.enabled   = true
+        docker.enabled         = false
+        singularity.enabled    = false
+        podman.enabled         = false
+        shifter.enabled        = false
+    }
+    test      { includeConfig 'conf/test.config'      }
+    test_full { includeConfig 'conf/test_full.config' }
 }
 
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container
 env {
-  PYTHONNOUSERSITE = 1
-  R_PROFILE_USER = "/.Rprofile"
-  R_ENVIRON_USER = "/.Renviron"
+    PYTHONNOUSERSITE = 1
+    R_PROFILE_USER   = "/.Rprofile"
+    R_ENVIRON_USER   = "/.Renviron"
 }
 
 // Capture exit codes from upstream processes when piping
@@ -132,61 +138,61 @@ process.shell = ['/bin/bash', '-euo', 'pipefail']
 
 def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
 timeline {
-  enabled = true
-  file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html"
+    enabled = true
+    file    = "${params.tracedir}/execution_timeline_${trace_timestamp}.html"
 }
 report {
-  enabled = true
-  file = "${params.tracedir}/execution_report_${trace_timestamp}.html"
+    enabled = true
+    file    = "${params.tracedir}/execution_report_${trace_timestamp}.html"
 }
 trace {
-  enabled = true
-  file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt"
+    enabled = true
+    file    = "${params.tracedir}/execution_trace_${trace_timestamp}.txt"
 }
 dag {
-  enabled = true
-  file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg"
+    enabled = true
+    file    = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg"
 }
 
 manifest {
-  name = 'nf-core/rnafusion'
-  author = 'Martin Proks'
-  homePage = 'https://github.com/nf-core/rnafusion'
-  description = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
-  mainScript = 'main.nf'
-  nextflowVersion = '>=20.04.0'
-  version = '1.3.0dev'
+    name            = 'nf-core/rnafusion'
+    author          = 'Martin Proks'
+    homePage        = 'https://github.com/nf-core/rnafusion'
+    description     = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
+    mainScript      = 'main.nf'
+    nextflowVersion = '!>=21.04.0'
+    version         = '1.3.0dev'
 }
 
 // Function to ensure that resource requirements don't go beyond
 // a maximum limit
 def check_max(obj, type) {
-  if (type == 'memory') {
-    try {
-      if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1)
-        return params.max_memory as nextflow.util.MemoryUnit
-      else
-        return obj
-    } catch (all) {
-      println "   ### ERROR ###   Max memory '${params.max_memory}' is not valid! Using default value: $obj"
-      return obj
-    }
-  } else if (type == 'time') {
-    try {
-      if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1)
-        return params.max_time as nextflow.util.Duration
-      else
-        return obj
-    } catch (all) {
-      println "   ### ERROR ###   Max time '${params.max_time}' is not valid! Using default value: $obj"
-      return obj
-    }
-  } else if (type == 'cpus') {
-    try {
-      return Math.min( obj, params.max_cpus as int )
-    } catch (all) {
-      println "   ### ERROR ###   Max cpus '${params.max_cpus}' is not valid! Using default value: $obj"
-      return obj
+    if (type == 'memory') {
+        try {
+            if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1)
+                return params.max_memory as nextflow.util.MemoryUnit
+            else
+                return obj
+        } catch (all) {
+            println "   ### ERROR ###   Max memory '${params.max_memory}' is not valid! Using default value: $obj"
+            return obj
+        }
+    } else if (type == 'time') {
+        try {
+            if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1)
+                return params.max_time as nextflow.util.Duration
+            else
+                return obj
+        } catch (all) {
+            println "   ### ERROR ###   Max time '${params.max_time}' is not valid! Using default value: $obj"
+            return obj
+        }
+    } else if (type == 'cpus') {
+        try {
+            return Math.min( obj, params.max_cpus as int )
+        } catch (all) {
+            println "   ### ERROR ###   Max cpus '${params.max_cpus}' is not valid! Using default value: $obj"
+            return obj
+        }
     }
-  }
 }
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 2d64558c..be45f425 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -16,19 +16,17 @@
             "properties": {
                 "input": {
                     "type": "string",
-                    "fa_icon": "fas fa-dna",
-                    "description": "Input FastQ files.",
-                    "help_text": "Use this to specify the location of your input FastQ files. For example:\n\n```bash\n--input 'path/to/data/sample_*_{1,2}.fastq'\n```\n\nPlease note the following requirements:\n\n1. The path must be enclosed in quotes\n2. The path must have at least one `*` wildcard character\n3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.\n\nIf left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`"
-                },
-                "single_end": {
-                    "type": "boolean",
-                    "description": "Specifies that the input is single-end reads.",
-                    "fa_icon": "fas fa-align-center",
-                    "help_text": "By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:\n\n```bash\n--single_end --input '*.fastq'\n```\n\nIt is not possible to run a mixture of single-end and paired-end files in one run."
+                    "format": "file-path",
+                    "mimetype": "text/csv",
+                    "pattern": "\\.csv$",
+                    "schema": "assets/schema_input.json",
+                    "description": "Path to comma-separated file containing information about the samples in the experiment.",
+                    "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/rnafusion/usage#samplesheet-input).",
+                    "fa_icon": "fas fa-file-csv"
                 },
                 "outdir": {
                     "type": "string",
-                    "description": "The output directory where the results will be saved.",
+                    "description": "Path to the output directory where the results will be saved.",
                     "default": "./results",
                     "fa_icon": "fas fa-folder-open"
                 },
@@ -38,6 +36,11 @@
                     "fa_icon": "fas fa-envelope",
                     "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.",
                     "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$"
+                },
+                "multiqc_title": {
+                    "type": "string",
+                    "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.",
+                    "fa_icon": "fas fa-file-signature"
                 }
             }
         },
@@ -45,22 +48,26 @@
             "title": "Reference genome options",
             "type": "object",
             "fa_icon": "fas fa-dna",
-            "description": "Options for the reference genome indices used to align reads.",
+            "description": "Reference genome related files and options required for the workflow.",
             "properties": {
                 "genome": {
                     "type": "string",
                     "description": "Name of iGenomes reference.",
                     "fa_icon": "fas fa-book",
-                    "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`.\n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details."
+                    "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details."
                 },
                 "fasta": {
                     "type": "string",
-                    "fa_icon": "fas fa-font",
+                    "format": "file-path",
+                    "mimetype": "text/plain",
+                    "pattern": "\\.fn?a(sta)?(\\.gz)?$",
                     "description": "Path to FASTA genome file.",
-                    "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible."
+                    "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.",
+                    "fa_icon": "far fa-file-code"
                 },
                 "igenomes_base": {
                     "type": "string",
+                    "format": "directory-path",
                     "description": "Directory / URL base for iGenomes references.",
                     "default": "s3://ngi-igenomes/igenomes",
                     "fa_icon": "fas fa-cloud-download-alt",
@@ -75,91 +82,57 @@
                 }
             }
         },
-        "generic_options": {
-            "title": "Generic options",
+        "institutional_config_options": {
+            "title": "Institutional config options",
             "type": "object",
-            "fa_icon": "fas fa-file-import",
-            "description": "Less common options for the pipeline, typically set in a config file.",
-            "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
+            "fa_icon": "fas fa-university",
+            "description": "Parameters used to describe centralised config profiles. These should not be edited.",
+            "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.",
             "properties": {
-                "help": {
-                    "type": "boolean",
-                    "description": "Display help text.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-question-circle"
-                },
-                "publish_dir_mode": {
+                "custom_config_version": {
                     "type": "string",
-                    "default": "copy",
+                    "description": "Git commit id for Institutional configs.",
+                    "default": "master",
                     "hidden": true,
-                    "description": "Method used to save pipeline results to output directory.",
-                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
-                    "fa_icon": "fas fa-copy",
-                    "enum": [
-                        "symlink",
-                        "rellink",
-                        "link",
-                        "copy",
-                        "copyNoFollow",
-                        "move"
-                    ]
-                },
-                "validate_params": {
-                    "type": "boolean",
-                    "description": "Boolean whether to validate parameters against the schema at runtime",
-                    "default": true,
-                    "fa_icon": "fas fa-check-square",
-                    "hidden": true
+                    "fa_icon": "fas fa-users-cog"
                 },
-                "email_on_fail": {
+                "custom_config_base": {
                     "type": "string",
-                    "description": "Email address for completion summary, only when pipeline fails.",
-                    "fa_icon": "fas fa-exclamation-triangle",
-                    "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$",
+                    "description": "Base directory for Institutional configs.",
+                    "default": "https://raw.githubusercontent.com/nf-core/configs/master",
                     "hidden": true,
-                    "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful."
+                    "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.",
+                    "fa_icon": "fas fa-users-cog"
                 },
-                "plaintext_email": {
-                    "type": "boolean",
-                    "description": "Send plain-text email instead of HTML.",
-                    "fa_icon": "fas fa-remove-format",
+                "hostnames": {
+                    "type": "string",
+                    "description": "Institutional configs hostname.",
                     "hidden": true,
-                    "help_text": "Set to receive plain-text e-mails instead of HTML formatted."
+                    "fa_icon": "fas fa-users-cog"
                 },
-                "max_multiqc_email_size": {
+                "config_profile_name": {
                     "type": "string",
-                    "description": "File size limit when attaching MultiQC reports to summary emails.",
-                    "default": "25.MB",
-                    "fa_icon": "fas fa-file-upload",
+                    "description": "Institutional config name.",
                     "hidden": true,
-                    "help_text": "If file generated by pipeline exceeds the threshold, it will not be attached."
+                    "fa_icon": "fas fa-users-cog"
                 },
-                "monochrome_logs": {
-                    "type": "boolean",
-                    "description": "Do not use coloured log outputs.",
-                    "fa_icon": "fas fa-palette",
+                "config_profile_description": {
+                    "type": "string",
+                    "description": "Institutional config description.",
                     "hidden": true,
-                    "help_text": "Set to disable colourful command line output and live life in monochrome."
+                    "fa_icon": "fas fa-users-cog"
                 },
-                "multiqc_config": {
+                "config_profile_contact": {
                     "type": "string",
-                    "description": "Custom config file to supply to MultiQC.",
-                    "fa_icon": "fas fa-cog",
-                    "hidden": true
+                    "description": "Institutional config contact information.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
                 },
-                "tracedir": {
+                "config_profile_url": {
                     "type": "string",
-                    "description": "Directory to keep pipeline Nextflow logs and reports.",
-                    "default": "${params.outdir}/pipeline_info",
-                    "fa_icon": "fas fa-cogs",
-                    "hidden": true
-                },
-                "show_hidden_params": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-eye-slash",
-                    "description": "Show all params when using `--help`",
+                    "description": "Institutional config URL link.",
                     "hidden": true,
-                    "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters."
+                    "fa_icon": "fas fa-users-cog"
                 }
             }
         },
@@ -172,7 +145,7 @@
             "properties": {
                 "max_cpus": {
                     "type": "integer",
-                    "description": "Maximum number of CPUs that can be requested    for any single job.",
+                    "description": "Maximum number of CPUs that can be requested for any single job.",
                     "default": 16,
                     "fa_icon": "fas fa-microchip",
                     "hidden": true,
@@ -198,58 +171,102 @@
                 }
             }
         },
-        "institutional_config_options": {
-            "title": "Institutional config options",
+        "generic_options": {
+            "title": "Generic options",
             "type": "object",
-            "fa_icon": "fas fa-university",
-            "description": "Parameters used to describe centralised config profiles. These should not be edited.",
-            "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.",
+            "fa_icon": "fas fa-file-import",
+            "description": "Less common options for the pipeline, typically set in a config file.",
+            "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
             "properties": {
-                "custom_config_version": {
+                "help": {
+                    "type": "boolean",
+                    "description": "Display help text.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "publish_dir_mode": {
                     "type": "string",
-                    "description": "Git commit id for Institutional configs.",
-                    "default": "master",
-                    "hidden": true,
-                    "fa_icon": "fas fa-users-cog",
-                    "help_text": "Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.\n\n```bash\n## Download and use config file with following git commit id\n--custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96\n```"
+                    "default": "copy",
+                    "description": "Method used to save pipeline results to output directory.",
+                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
+                    "fa_icon": "fas fa-copy",
+                    "enum": [
+                        "symlink",
+                        "rellink",
+                        "link",
+                        "copy",
+                        "copyNoFollow",
+                        "move"
+                    ],
+                    "hidden": true
                 },
-                "custom_config_base": {
+                "email_on_fail": {
                     "type": "string",
-                    "description": "Base directory for Institutional configs.",
-                    "default": "https://raw.githubusercontent.com/nf-core/configs/master",
-                    "hidden": true,
-                    "help_text": "If you're running offline, nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell nextflow where to find them with the `custom_config_base` option. For example:\n\n```bash\n## Download and unzip the config files\ncd /path/to/my/configs\nwget https://github.com/nf-core/configs/archive/master.zip\nunzip master.zip\n\n## Run the pipeline\ncd /path/to/my/data\nnextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/\n```\n\n> Note that the nf-core/tools helper package has a `download` command to download all required pipeline files + singularity containers + institutional configs in one go for you, to make this process easier.",
-                    "fa_icon": "fas fa-users-cog"
+                    "description": "Email address for completion summary, only when pipeline fails.",
+                    "fa_icon": "fas fa-exclamation-triangle",
+                    "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$",
+                    "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.",
+                    "hidden": true
                 },
-                "hostnames": {
+                "plaintext_email": {
+                    "type": "boolean",
+                    "description": "Send plain-text email instead of HTML.",
+                    "fa_icon": "fas fa-remove-format",
+                    "hidden": true
+                },
+                "max_multiqc_email_size": {
                     "type": "string",
-                    "description": "Institutional configs hostname.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
+                    "description": "File size limit when attaching MultiQC reports to summary emails.",
+                    "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$",
+                    "default": "25.MB",
+                    "fa_icon": "fas fa-file-upload",
+                    "hidden": true
                 },
-                "config_profile_name": {
+                "monochrome_logs": {
+                    "type": "boolean",
+                    "description": "Do not use coloured log outputs.",
+                    "fa_icon": "fas fa-palette",
+                    "hidden": true
+                },
+                "multiqc_config": {
                     "type": "string",
-                    "description": "Institutional config name.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
+                    "description": "Custom config file to supply to MultiQC.",
+                    "fa_icon": "fas fa-cog",
+                    "hidden": true
                 },
-                "config_profile_description": {
+                "tracedir": {
                     "type": "string",
-                    "description": "Institutional config description.",
+                    "description": "Directory to keep pipeline Nextflow logs and reports.",
+                    "default": "${params.outdir}/pipeline_info",
+                    "fa_icon": "fas fa-cogs",
+                    "hidden": true
+                },
+                "validate_params": {
+                    "type": "boolean",
+                    "description": "Boolean whether to validate parameters against the schema at runtime",
+                    "default": true,
+                    "fa_icon": "fas fa-check-square",
+                    "hidden": true
+                },
+                "show_hidden_params": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-eye-slash",
+                    "description": "Show all params when using `--help`",
                     "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
+                    "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters."
                 },
-                "config_profile_contact": {
-                    "type": "string",
-                    "description": "Institutional config contact information.",
+                "enable_conda": {
+                    "type": "boolean",
+                    "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.",
                     "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
+                    "fa_icon": "fas fa-bacon"
                 },
-                "config_profile_url": {
-                    "type": "string",
-                    "description": "Institutional config URL link.",
+                "singularity_pull_docker_container": {
+                    "type": "boolean",
+                    "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.",
                     "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues."
                 }
             }
         }
@@ -262,13 +279,13 @@
             "$ref": "#/definitions/reference_genome_options"
         },
         {
-            "$ref": "#/definitions/generic_options"
+            "$ref": "#/definitions/institutional_config_options"
         },
         {
             "$ref": "#/definitions/max_job_request_options"
         },
         {
-            "$ref": "#/definitions/institutional_config_options"
+            "$ref": "#/definitions/generic_options"
         }
     ]
 }
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
new file mode 100644
index 00000000..b664bc8c
--- /dev/null
+++ b/subworkflows/local/input_check.nf
@@ -0,0 +1,42 @@
+//
+// Check input samplesheet and get read channels
+//
+
+params.options = [:]
+
+include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options )
+
+workflow INPUT_CHECK {
+    take:
+    samplesheet // file: /path/to/samplesheet.csv
+
+    main:
+    SAMPLESHEET_CHECK ( samplesheet )
+        .splitCsv ( header:true, sep:',' )
+        .map { create_fastq_channels(it) }
+        .set { reads }
+
+    emit:
+    reads // channel: [ val(meta), [ reads ] ]
+}
+
+// Function to get list of [ meta, [ fastq_1, fastq_2 ] ]
+def create_fastq_channels(LinkedHashMap row) {
+    def meta = [:]
+    meta.id           = row.sample
+    meta.single_end   = row.single_end.toBoolean()
+
+    def array = []
+    if (!file(row.fastq_1).exists()) {
+        exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}"
+    }
+    if (meta.single_end) {
+        array = [ meta, [ file(row.fastq_1) ] ]
+    } else {
+        if (!file(row.fastq_2).exists()) {
+            exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}"
+        }
+        array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ]
+    }
+    return array
+}
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
new file mode 100644
index 00000000..0728b486
--- /dev/null
+++ b/workflows/rnafusion.nf
@@ -0,0 +1,141 @@
+/*
+========================================================================================
+    VALIDATE INPUTS
+========================================================================================
+*/
+
+def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
+
+// Validate input parameters
+WorkflowRnafusion.initialise(params, log)
+
+// TODO nf-core: Add all file path parameters for the pipeline to the list below
+// Check input path parameters to see if they exist
+def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ]
+for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
+
+// Check mandatory parameters
+if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' }
+
+/*
+========================================================================================
+    CONFIG FILES
+========================================================================================
+*/
+
+ch_multiqc_config        = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true)
+ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
+
+/*
+========================================================================================
+    IMPORT LOCAL MODULES/SUBWORKFLOWS
+========================================================================================
+*/
+
+// Don't overwrite global params.modules, create a copy instead and use that within the main script.
+def modules = params.modules.clone()
+
+//
+// MODULE: Local to the pipeline
+//
+include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] )
+
+//
+// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
+//
+include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] )
+
+/*
+========================================================================================
+    IMPORT NF-CORE MODULES/SUBWORKFLOWS
+========================================================================================
+*/
+
+def multiqc_options   = modules['multiqc']
+multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : ''
+
+//
+// MODULE: Installed directly from nf-core/modules
+//
+include { FASTQC  } from '../modules/nf-core/modules/fastqc/main'  addParams( options: modules['fastqc'] )
+include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options   )
+
+/*
+========================================================================================
+    RUN MAIN WORKFLOW
+========================================================================================
+*/
+
+// Info required for completion email and summary
+def multiqc_report = []
+
+workflow RNAFUSION {
+
+    ch_software_versions = Channel.empty()
+
+    //
+    // SUBWORKFLOW: Read in samplesheet, validate and stage input files
+    //
+    INPUT_CHECK (
+        ch_input
+    )
+
+    //
+    // MODULE: Run FastQC
+    //
+    FASTQC (
+        INPUT_CHECK.out.reads
+    )
+    ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null))
+
+    //
+    // MODULE: Pipeline reporting
+    //
+    ch_software_versions
+        .map { it -> if (it) [ it.baseName, it ] }
+        .groupTuple()
+        .map { it[1][0] }
+        .flatten()
+        .collect()
+        .set { ch_software_versions }
+
+    GET_SOFTWARE_VERSIONS (
+        ch_software_versions.map { it }.collect()
+    )
+
+    //
+    // MODULE: MultiQC
+    //
+    workflow_summary    = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params)
+    ch_workflow_summary = Channel.value(workflow_summary)
+
+    ch_multiqc_files = Channel.empty()
+    ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect())
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+
+    MULTIQC (
+        ch_multiqc_files.collect()
+    )
+    multiqc_report       = MULTIQC.out.report.toList()
+    ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null))
+}
+
+/*
+========================================================================================
+    COMPLETION EMAIL AND SUMMARY
+========================================================================================
+*/
+
+workflow.onComplete {
+    NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
+    NfcoreTemplate.summary(workflow, params, log)
+}
+
+/*
+========================================================================================
+    THE END
+========================================================================================
+*/

From d090d437a2a885d4a6687e56aa2e122bd946e60b Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 13 Jul 2021 15:33:02 +0000
Subject: [PATCH 028/359] Template update for nf-core/tools version 2.0.1

---
 .github/workflows/linting.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 13b4fc81..fbde5cf8 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -127,7 +127,7 @@ jobs:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
-        run: nf-core -l lint_log.txt lint ${GITHUB_WORKSPACE} --markdown lint_results.md
+        run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
 
       - name: Save PR number
         if: ${{ always() }}

From c9937ade5714396a902bbb047d838912207b6d5b Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 27 Jul 2021 16:09:34 +0000
Subject: [PATCH 029/359] Template update for nf-core/tools version 2.1

---
 .editorconfig                    |  3 +++
 .github/CONTRIBUTING.md          |  6 +++---
 .github/PULL_REQUEST_TEMPLATE.md |  2 +-
 .github/workflows/linting.yml    |  2 +-
 README.md                        |  2 +-
 bin/scrape_software_versions.py  |  2 +-
 lib/NfcoreTemplate.groovy        | 22 +++++++++++++---------
 nextflow_schema.json             |  4 ++--
 workflows/rnafusion.nf           |  4 +++-
 9 files changed, 28 insertions(+), 19 deletions(-)

diff --git a/.editorconfig b/.editorconfig
index afb20bb1..95549501 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -11,6 +11,9 @@ indent_style = space
 [*.{yml,yaml}]
 indent_size = 2
 
+[*.json]
+insert_final_newline = unset
+
 # These files are edited and tested upstream in nf-core/modules
 [/modules/nf-core/**]
 charset = unset
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 06a1bbd5..1912ed9d 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -19,7 +19,7 @@ If you'd like to write some code for nf-core/rnafusion, the standard workflow is
     * If there isn't one already, please create one so that others know you're working on this
 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rnafusion repository](https://github.com/nf-core/rnafusion) to your GitHub account
 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
-4. Use `nf-core schema build .` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
+4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged
 
 If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/).
@@ -69,7 +69,7 @@ If you wish to contribute a new step, please use the following coding standards:
 2. Write the process block (see below).
 3. Define the output channel if needed (see below).
 4. Add any new flags/options to `nextflow.config` with a default (see below).
-5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`).
+5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build`).
 6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter).
 7. Add sanity checks for all relevant parameters.
 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`.
@@ -83,7 +83,7 @@ If you wish to contribute a new step, please use the following coding standards:
 
 Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope.
 
-Once there, use `nf-core schema build .` to add to `nextflow_schema.json`.
+Once there, use `nf-core schema build` to add to `nextflow_schema.json`.
 
 ### Default processes resource requirements
 
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 3760ac7b..026428de 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
     - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
     - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
-- [ ] Make sure your code lints (`nf-core lint .`).
+- [ ] Make sure your code lints (`nf-core lint`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index fbde5cf8..3b448773 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -53,7 +53,7 @@ jobs:
 
       - uses: actions/setup-node@v1
         with:
-          node-version: "10"
+          node-version: '10'
 
       - name: Install editorconfig-checker
         run: npm install -g editorconfig-checker
diff --git a/README.md b/README.md
index 41023216..f65ac7b7 100644
--- a/README.md
+++ b/README.md
@@ -33,7 +33,7 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 ## Quick Start
 
-1. Install [`Nextflow`](https://nf-co.re/usage/installation) (`>=21.04.0`)
+1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.04.0`)
 
 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
 
diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
index 590af3bb..ee132097 100755
--- a/bin/scrape_software_versions.py
+++ b/bin/scrape_software_versions.py
@@ -30,7 +30,7 @@
     print("        <dt>{}</dt><dd><samp>{}</samp></dd>".format(k, v))
 print("    </dl>")
 
-# Write out regexes as csv file:
+# Write out as tsv file:
 with open("software_versions.tsv", "w") as f:
     for k, v in sorted(results.items()):
         f.write("{}\t{}\n".format(k, v))
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index b6e689ec..44551e0a 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -24,17 +24,21 @@ class NfcoreTemplate {
     public static void hostName(workflow, params, log) {
         Map colors = logColours(params.monochrome_logs)
         if (params.hostnames) {
-            def hostname = "hostname".execute().text.trim()
-            params.hostnames.each { prof, hnames ->
-                hnames.each { hname ->
-                    if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                        log.info "=${colors.yellow}====================================================${colors.reset}=\n" +
-                            "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" +
-                            "      but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" +
-                            "      ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" +
-                            "=${colors.yellow}====================================================${colors.reset}="
+            try {
+                def hostname = "hostname".execute().text.trim()
+                params.hostnames.each { prof, hnames ->
+                    hnames.each { hname ->
+                        if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
+                            log.info "=${colors.yellow}====================================================${colors.reset}=\n" +
+                                "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" +
+                                "      but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" +
+                                "      ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" +
+                                "=${colors.yellow}====================================================${colors.reset}="
+                        }
                     }
                 }
+            } catch (Exception e) {
+                log.warn "[$workflow.manifest.name] Could not determine 'hostname' - skipping check. Reason: ${e.message}."
             }
         }
     }
diff --git a/nextflow_schema.json b/nextflow_schema.json
index be45f425..af484875 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -18,7 +18,7 @@
                     "type": "string",
                     "format": "file-path",
                     "mimetype": "text/csv",
-                    "pattern": "\\.csv$",
+                    "pattern": "^\\S+\\.csv$",
                     "schema": "assets/schema_input.json",
                     "description": "Path to comma-separated file containing information about the samples in the experiment.",
                     "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/rnafusion/usage#samplesheet-input).",
@@ -60,7 +60,7 @@
                     "type": "string",
                     "format": "file-path",
                     "mimetype": "text/plain",
-                    "pattern": "\\.fn?a(sta)?(\\.gz)?$",
+                    "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",
                     "description": "Path to FASTA genome file.",
                     "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.",
                     "fa_icon": "far fa-file-code"
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 0728b486..10879efe 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -130,7 +130,9 @@ workflow RNAFUSION {
 */
 
 workflow.onComplete {
-    NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
+    if (params.email || params.email_on_fail) {
+        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
+    }
     NfcoreTemplate.summary(workflow, params, log)
 }
 

From 8e2b12f0439844bec74832469cb0da0dfb73457b Mon Sep 17 00:00:00 2001
From: praveen <pravee1216@gmail.com>
Date: Thu, 29 Jul 2021 11:23:33 +0200
Subject: [PATCH 030/359] initial template build from nf-core/tools, version
 2.0.1

---
 .editorconfig                                |  24 +
 .gitattributes                               |   1 +
 .github/.dockstore.yml                       |   6 +
 .github/CONTRIBUTING.md                      | 128 +++++
 .github/ISSUE_TEMPLATE/bug_report.md         |  63 +++
 .github/ISSUE_TEMPLATE/config.yml            |   8 +
 .github/ISSUE_TEMPLATE/feature_request.md    |  32 ++
 .github/PULL_REQUEST_TEMPLATE.md             |  26 +
 .github/workflows/awsfulltest.yml            |  34 ++
 .github/workflows/awstest.yml                |  28 +
 .github/workflows/branch.yml                 |  46 ++
 .github/workflows/ci.yml                     |  43 ++
 .github/workflows/linting.yml                | 145 ++++++
 .github/workflows/linting_comment.yml        |  29 ++
 .gitignore                                   |   8 +
 .markdownlint.yml                            |  14 +
 CHANGELOG.md                                 |  16 +
 CITATIONS.md                                 |  32 ++
 CODE_OF_CONDUCT.md                           | 111 ++++
 LICENSE                                      |  21 +
 README.md                                    |  90 ++++
 assets/email_template.html                   |  53 ++
 assets/email_template.txt                    |  40 ++
 assets/multiqc_config.yaml                   |  11 +
 assets/nf-core-rnafusion_logo.png            | Bin 0 -> 18138 bytes
 assets/samplesheet.csv                       |   3 +
 assets/schema_input.json                     |  39 ++
 assets/sendmail_template.txt                 |  53 ++
 bin/check_samplesheet.py                     | 146 ++++++
 bin/scrape_software_versions.py              |  36 ++
 conf/base.config                             |  57 ++
 conf/igenomes.config                         | 432 ++++++++++++++++
 conf/modules.config                          |  32 ++
 conf/test.config                             |  29 ++
 conf/test_full.config                        |  24 +
 docs/README.md                               |  10 +
 docs/images/mqc_fastqc_adapter.png           | Bin 0 -> 23458 bytes
 docs/images/mqc_fastqc_counts.png            | Bin 0 -> 33918 bytes
 docs/images/mqc_fastqc_quality.png           | Bin 0 -> 55769 bytes
 docs/images/nf-core-rnafusion_logo.png       | Bin 0 -> 35022 bytes
 docs/output.md                               |  68 +++
 docs/usage.md                                | 284 ++++++++++
 lib/NfcoreSchema.groovy                      | 517 +++++++++++++++++++
 lib/NfcoreTemplate.groovy                    | 266 ++++++++++
 lib/Utils.groovy                             |  47 ++
 lib/WorkflowMain.groovy                      |  94 ++++
 lib/WorkflowRnafusion.groovy                 |  59 +++
 lib/nfcore_external_java_deps.jar            | Bin 0 -> 2291171 bytes
 main.nf                                      |  63 +++
 modules.json                                 |  14 +
 modules/local/functions.nf                   |  68 +++
 modules/local/get_software_versions.nf       |  33 ++
 modules/local/samplesheet_check.nf           |  31 ++
 modules/nf-core/modules/fastqc/functions.nf  |  68 +++
 modules/nf-core/modules/fastqc/main.nf       |  47 ++
 modules/nf-core/modules/fastqc/meta.yml      |  51 ++
 modules/nf-core/modules/multiqc/functions.nf |  68 +++
 modules/nf-core/modules/multiqc/main.nf      |  35 ++
 modules/nf-core/modules/multiqc/meta.yml     |  39 ++
 nextflow.config                              | 198 +++++++
 nextflow_schema.json                         | 291 +++++++++++
 subworkflows/local/input_check.nf            |  42 ++
 workflows/rnafusion.nf                       | 141 +++++
 63 files changed, 4394 insertions(+)
 create mode 100644 .editorconfig
 create mode 100644 .gitattributes
 create mode 100644 .github/.dockstore.yml
 create mode 100644 .github/CONTRIBUTING.md
 create mode 100644 .github/ISSUE_TEMPLATE/bug_report.md
 create mode 100644 .github/ISSUE_TEMPLATE/config.yml
 create mode 100644 .github/ISSUE_TEMPLATE/feature_request.md
 create mode 100644 .github/PULL_REQUEST_TEMPLATE.md
 create mode 100644 .github/workflows/awsfulltest.yml
 create mode 100644 .github/workflows/awstest.yml
 create mode 100644 .github/workflows/branch.yml
 create mode 100644 .github/workflows/ci.yml
 create mode 100644 .github/workflows/linting.yml
 create mode 100644 .github/workflows/linting_comment.yml
 create mode 100644 .gitignore
 create mode 100644 .markdownlint.yml
 create mode 100644 CHANGELOG.md
 create mode 100644 CITATIONS.md
 create mode 100644 CODE_OF_CONDUCT.md
 create mode 100644 LICENSE
 create mode 100644 README.md
 create mode 100644 assets/email_template.html
 create mode 100644 assets/email_template.txt
 create mode 100644 assets/multiqc_config.yaml
 create mode 100644 assets/nf-core-rnafusion_logo.png
 create mode 100644 assets/samplesheet.csv
 create mode 100644 assets/schema_input.json
 create mode 100644 assets/sendmail_template.txt
 create mode 100755 bin/check_samplesheet.py
 create mode 100755 bin/scrape_software_versions.py
 create mode 100644 conf/base.config
 create mode 100644 conf/igenomes.config
 create mode 100644 conf/modules.config
 create mode 100644 conf/test.config
 create mode 100644 conf/test_full.config
 create mode 100644 docs/README.md
 create mode 100755 docs/images/mqc_fastqc_adapter.png
 create mode 100755 docs/images/mqc_fastqc_counts.png
 create mode 100755 docs/images/mqc_fastqc_quality.png
 create mode 100644 docs/images/nf-core-rnafusion_logo.png
 create mode 100644 docs/output.md
 create mode 100644 docs/usage.md
 create mode 100755 lib/NfcoreSchema.groovy
 create mode 100755 lib/NfcoreTemplate.groovy
 create mode 100755 lib/Utils.groovy
 create mode 100755 lib/WorkflowMain.groovy
 create mode 100755 lib/WorkflowRnafusion.groovy
 create mode 100644 lib/nfcore_external_java_deps.jar
 create mode 100644 main.nf
 create mode 100644 modules.json
 create mode 100644 modules/local/functions.nf
 create mode 100644 modules/local/get_software_versions.nf
 create mode 100644 modules/local/samplesheet_check.nf
 create mode 100644 modules/nf-core/modules/fastqc/functions.nf
 create mode 100644 modules/nf-core/modules/fastqc/main.nf
 create mode 100644 modules/nf-core/modules/fastqc/meta.yml
 create mode 100644 modules/nf-core/modules/multiqc/functions.nf
 create mode 100644 modules/nf-core/modules/multiqc/main.nf
 create mode 100644 modules/nf-core/modules/multiqc/meta.yml
 create mode 100644 nextflow.config
 create mode 100644 nextflow_schema.json
 create mode 100644 subworkflows/local/input_check.nf
 create mode 100644 workflows/rnafusion.nf

diff --git a/.editorconfig b/.editorconfig
new file mode 100644
index 00000000..afb20bb1
--- /dev/null
+++ b/.editorconfig
@@ -0,0 +1,24 @@
+root = true
+
+[*]
+charset = utf-8
+end_of_line = lf
+insert_final_newline = true
+trim_trailing_whitespace = true
+indent_size = 4
+indent_style = space
+
+[*.{yml,yaml}]
+indent_size = 2
+
+# These files are edited and tested upstream in nf-core/modules
+[/modules/nf-core/**]
+charset = unset
+end_of_line = unset
+insert_final_newline = unset
+trim_trailing_whitespace = unset
+indent_style = unset
+indent_size = unset
+
+[/assets/email*]
+indent_size = unset
diff --git a/.gitattributes b/.gitattributes
new file mode 100644
index 00000000..7fe55006
--- /dev/null
+++ b/.gitattributes
@@ -0,0 +1 @@
+*.config linguist-language=nextflow
diff --git a/.github/.dockstore.yml b/.github/.dockstore.yml
new file mode 100644
index 00000000..191fabd2
--- /dev/null
+++ b/.github/.dockstore.yml
@@ -0,0 +1,6 @@
+# Dockstore config version, not pipeline version
+version: 1.2
+workflows:
+  - subclass: nfl
+    primaryDescriptorPath: /nextflow.config
+    publish: True
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
new file mode 100644
index 00000000..06a1bbd5
--- /dev/null
+++ b/.github/CONTRIBUTING.md
@@ -0,0 +1,128 @@
+# nf-core/rnafusion: Contributing Guidelines
+
+Hi there!
+Many thanks for taking an interest in improving nf-core/rnafusion.
+
+We try to manage the required tasks for nf-core/rnafusion using GitHub issues, you probably came to this page when creating one.
+Please use the pre-filled template to save time.
+
+However, don't be put off by this template - other more general issues and suggestions are welcome!
+Contributions to the code are even more welcome ;)
+
+> If you need help using or modifying nf-core/rnafusion then the best place to ask is on the nf-core Slack [#rnafusion](https://nfcore.slack.com/channels/rnafusion) channel ([join our Slack here](https://nf-co.re/join/slack)).
+
+## Contribution workflow
+
+If you'd like to write some code for nf-core/rnafusion, the standard workflow is as follows:
+
+1. Check that there isn't already an issue about your idea in the [nf-core/rnafusion issues](https://github.com/nf-core/rnafusion/issues) to avoid duplicating work
+    * If there isn't one already, please create one so that others know you're working on this
+2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rnafusion repository](https://github.com/nf-core/rnafusion) to your GitHub account
+3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
+4. Use `nf-core schema build .` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
+5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged
+
+If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/).
+
+## Tests
+
+When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests.
+Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then.
+
+There are typically two types of tests that run:
+
+### Lint tests
+
+`nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to.
+To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint <pipeline-directory>` command.
+
+If any failures or warnings are encountered, please follow the listed URL for more documentation.
+
+### Pipeline tests
+
+Each `nf-core` pipeline should be set up with a minimal set of test-data.
+`GitHub Actions` then runs the pipeline on this data to ensure that it exits successfully.
+If there are any failures then the automated tests fail.
+These tests are run both with the latest available version of `Nextflow` and also the minimum required version that is stated in the pipeline code.
+
+## Patch
+
+:warning: Only in the unlikely and regretful event of a release happening with a bug.
+
+* On your own fork, make a new branch `patch` based on `upstream/master`.
+* Fix the bug, and bump version (X.Y.Z+1).
+* A PR should be made on `master` from patch to directly this particular bug.
+
+## Getting help
+
+For further information/help, please consult the [nf-core/rnafusion documentation](https://nf-co.re/rnafusion/usage) and don't hesitate to get in touch on the nf-core Slack [#rnafusion](https://nfcore.slack.com/channels/rnafusion) channel ([join our Slack here](https://nf-co.re/join/slack)).
+
+## Pipeline contribution conventions
+
+To make the nf-core/rnafusion code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written.
+
+### Adding a new step
+
+If you wish to contribute a new step, please use the following coding standards:
+
+1. Define the corresponding input channel into your new process from the expected previous process channel
+2. Write the process block (see below).
+3. Define the output channel if needed (see below).
+4. Add any new flags/options to `nextflow.config` with a default (see below).
+5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`).
+6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter).
+7. Add sanity checks for all relevant parameters.
+8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`.
+9. Do local tests that the new code works properly and as expected.
+10. Add a new test command in `.github/workflow/ci.yml`.
+11. If applicable add a [MultiQC](https://https://multiqc.info/) module.
+12. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, name clean up, General Statistics Table column order, and module figures are in the right order.
+13. Optional: Add any descriptions of MultiQC report sections and output files to `docs/output.md`.
+
+### Default values
+
+Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope.
+
+Once there, use `nf-core schema build .` to add to `nextflow_schema.json`.
+
+### Default processes resource requirements
+
+Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
+
+The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block.
+
+### Naming schemes
+
+Please use the following naming schemes, to make it easy to understand what is going where.
+
+* initial process channel: `ch_output_from_<process>`
+* intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
+
+### Nextflow version bumping
+
+If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]`
+
+### Software version reporting
+
+If you add a new tool to the pipeline, please ensure you add the information of the tool to the `get_software_version` process.
+
+Add to the script block of the process, something like the following:
+
+```bash
+<YOUR_TOOL> --version &> v_<YOUR_TOOL>.txt 2>&1 || true
+```
+
+or
+
+```bash
+<YOUR_TOOL> --help | head -n 1 &> v_<YOUR_TOOL>.txt 2>&1 || true
+```
+
+You then need to edit the script `bin/scrape_software_versions.py` to:
+
+1. Add a Python regex for your tool's `--version` output (as in stored in the `v_<YOUR_TOOL>.txt` file), to ensure the version is reported as a `v` and the version number e.g. `v2.1.1`
+2. Add a HTML entry to the `OrderedDict` for formatting in MultiQC.
+
+### Images and figures
+
+For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines).
diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md
new file mode 100644
index 00000000..21b44f28
--- /dev/null
+++ b/.github/ISSUE_TEMPLATE/bug_report.md
@@ -0,0 +1,63 @@
+---
+name: Bug report
+about: Report something that is broken or incorrect
+labels: bug
+---
+
+<!--
+# nf-core/rnafusion bug report
+
+Hi there!
+
+Thanks for telling us about a problem with the pipeline.
+Please delete this text and anything that's not relevant from the template below:
+-->
+
+## Check Documentation
+
+I have checked the following places for your error:
+
+- [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
+- [ ] [nf-core/rnafusion pipeline documentation](https://nf-co.re/rnafusion/usage)
+
+## Description of the bug
+
+<!-- A clear and concise description of what the bug is. -->
+
+## Steps to reproduce
+
+Steps to reproduce the behaviour:
+
+1. Command line: <!-- [e.g. `nextflow run ...`] -->
+2. See error: <!-- [Please provide your error message] -->
+
+## Expected behaviour
+
+<!-- A clear and concise description of what you expected to happen. -->
+
+## Log files
+
+Have you provided the following extra information/files:
+
+- [ ] The command used to run the pipeline
+- [ ] The `.nextflow.log` file <!-- this is a hidden file in the directory where you launched the pipeline -->
+
+## System
+
+- Hardware: <!-- [e.g. HPC, Desktop, Cloud...] -->
+- Executor: <!-- [e.g. slurm, local, awsbatch...] -->
+- OS: <!-- [e.g. CentOS Linux, macOS, Linux Mint...] -->
+- Version <!-- [e.g. 7, 10.13.6, 18.3...] -->
+
+## Nextflow Installation
+
+- Version: <!-- [e.g. 21.04.0] -->
+
+## Container engine
+
+- Engine: <!-- [e.g. Conda, Docker, Singularity, Podman, Shifter or Charliecloud] -->
+- version: <!-- [e.g. 1.0.0] -->
+
+## Additional context
+
+<!-- Add any other context about the problem here. -->
diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml
new file mode 100644
index 00000000..1fccc741
--- /dev/null
+++ b/.github/ISSUE_TEMPLATE/config.yml
@@ -0,0 +1,8 @@
+blank_issues_enabled: false
+contact_links:
+  - name: Join nf-core
+    url: https://nf-co.re/join
+    about: Please join the nf-core community here
+  - name: "Slack #rnafusion channel"
+    url: https://nfcore.slack.com/channels/rnafusion
+    about: Discussion about the nf-core/rnafusion pipeline
diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md
new file mode 100644
index 00000000..10da2c83
--- /dev/null
+++ b/.github/ISSUE_TEMPLATE/feature_request.md
@@ -0,0 +1,32 @@
+---
+name: Feature request
+about: Suggest an idea for the nf-core/rnafusion pipeline
+labels: enhancement
+---
+
+<!--
+# nf-core/rnafusion feature request
+
+Hi there!
+
+Thanks for suggesting a new feature for the pipeline!
+Please delete this text and anything that's not relevant from the template below:
+-->
+
+## Is your feature request related to a problem? Please describe
+
+<!-- A clear and concise description of what the problem is. -->
+
+<!-- e.g. [I'm always frustrated when ...] -->
+
+## Describe the solution you'd like
+
+<!-- A clear and concise description of what you want to happen. -->
+
+## Describe alternatives you've considered
+
+<!-- A clear and concise description of any alternative solutions or features you've considered. -->
+
+## Additional context
+
+<!-- Add any other context about the feature request here. -->
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
new file mode 100644
index 00000000..3760ac7b
--- /dev/null
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -0,0 +1,26 @@
+<!--
+# nf-core/rnafusion pull request
+
+Many thanks for contributing to nf-core/rnafusion!
+
+Please fill in the appropriate checklist below (delete whatever is not relevant).
+These are the most common things requested on pull requests (PRs).
+
+Remember that PRs should be made against the dev branch, unless you're preparing a pipeline release.
+
+Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+-->
+<!-- markdownlint-disable ul-indent -->
+
+## PR checklist
+
+- [ ] This comment contains a description of changes (with reason).
+- [ ] If you've fixed a bug or added code that should be tested, add tests!
+    - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+    - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
+- [ ] Make sure your code lints (`nf-core lint .`).
+- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
+- [ ] Usage Documentation in `docs/usage.md` is updated.
+- [ ] Output Documentation in `docs/output.md` is updated.
+- [ ] `CHANGELOG.md` is updated.
+- [ ] `README.md` is updated (including new tool citations and authors/contributors).
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
new file mode 100644
index 00000000..ed90a293
--- /dev/null
+++ b/.github/workflows/awsfulltest.yml
@@ -0,0 +1,34 @@
+name: nf-core AWS full size tests
+# This workflow is triggered on published releases.
+# It can be additionally triggered manually with GitHub actions workflow dispatch button.
+# It runs the -profile 'test_full' on AWS batch
+
+on:
+  release:
+    types: [published]
+  workflow_dispatch:
+jobs:
+  run-tower:
+    name: Run AWS full tests
+    if: github.repository == 'nf-core/rnafusion'
+    runs-on: ubuntu-latest
+    steps:
+      - name: Launch workflow via tower
+        uses: nf-core/tower-action@master
+        # TODO nf-core: You can customise AWS full pipeline tests as required
+        # Add full size test data (but still relatively small datasets for few samples)
+        # on the `test_full.config` test runs with only one set of parameters
+        
+        with:
+          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
+          bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }}
+          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
+          pipeline: ${{ github.repository }}
+          revision: ${{ github.sha }}
+          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
+          parameters: |
+            {
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
+            }
+          profiles: '[ "test_full", "aws_tower" ]'
+
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
new file mode 100644
index 00000000..c3b9fbb4
--- /dev/null
+++ b/.github/workflows/awstest.yml
@@ -0,0 +1,28 @@
+name: nf-core AWS test
+# This workflow can be triggered manually with the GitHub actions workflow dispatch button.
+# It runs the -profile 'test' on AWS batch
+
+on:
+  workflow_dispatch:
+jobs:
+  run-tower:
+    name: Run AWS tests
+    if: github.repository == 'nf-core/rnafusion'
+    runs-on: ubuntu-latest
+    steps:
+      - name: Launch workflow via tower
+        uses: nf-core/tower-action@master
+        
+        with:
+          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
+          bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }}
+          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
+          pipeline: ${{ github.repository }}
+          revision: ${{ github.sha }}
+          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
+          parameters: |
+            {
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
+            }
+          profiles: '[ "test", "aws_tower" ]'
+
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
new file mode 100644
index 00000000..37b7dea8
--- /dev/null
+++ b/.github/workflows/branch.yml
@@ -0,0 +1,46 @@
+name: nf-core branch protection
+# This workflow is triggered on PRs to master branch on the repository
+# It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev`
+on:
+  pull_request_target:
+    branches: [master]
+
+jobs:
+  test:
+    runs-on: ubuntu-latest
+    steps:
+      # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
+      - name: Check PRs
+        if: github.repository == 'nf-core/rnafusion'
+        run: |
+          { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
+
+
+      # If the above check failed, post a comment on the PR explaining the failure
+      # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
+      - name: Post PR comment
+        if: failure()
+        uses: mshick/add-pr-comment@v1
+        with:
+          message: |
+            ## This PR is against the `master` branch :x:
+
+            * Do not close this PR
+            * Click _Edit_ and change the `base` to `dev`
+            * This CI test will remain failed until you push a new commit
+
+            ---
+
+            Hi @${{ github.event.pull_request.user.login }},
+
+            It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch.
+            The `master` branch on nf-core repositories should always contain code from the latest release.
+            Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
+
+            You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page.
+            Note that even after this, the test will continue to show as failing until you push a new commit.
+
+            Thanks again for your contribution!
+          repo-token: ${{ secrets.GITHUB_TOKEN }}
+          allow-repeats: false
+
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
new file mode 100644
index 00000000..1145652b
--- /dev/null
+++ b/.github/workflows/ci.yml
@@ -0,0 +1,43 @@
+name: nf-core CI
+# This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors
+on:
+  push:
+    branches:
+      - dev
+  pull_request:
+  release:
+    types: [published]
+
+# Uncomment if we need an edge release of Nextflow again
+# env: NXF_EDGE: 1
+
+jobs:
+  test:
+    name: Run workflow tests
+    # Only run on push if this is the nf-core dev branch (merged PRs)
+    if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}
+    runs-on: ubuntu-latest
+    env:
+      NXF_VER: ${{ matrix.nxf_ver }}
+      NXF_ANSI_LOG: false
+    strategy:
+      matrix:
+        # Nextflow versions: check pipeline minimum and current latest
+        nxf_ver: ['21.04.0', '']
+    steps:
+      - name: Check out pipeline code
+        uses: actions/checkout@v2
+
+      - name: Install Nextflow
+        env:
+          CAPSULE_LOG: none
+        run: |
+          wget -qO- get.nextflow.io | bash
+          sudo mv nextflow /usr/local/bin/
+
+      - name: Run pipeline with test data
+        # TODO nf-core: You can customise CI pipeline run tests as required
+        # For example: adding multiple test runs with different parameters
+        # Remember that you can parallelise this by using strategy.matrix
+        run: |
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
new file mode 100644
index 00000000..fbde5cf8
--- /dev/null
+++ b/.github/workflows/linting.yml
@@ -0,0 +1,145 @@
+name: nf-core linting
+# This workflow is triggered on pushes and PRs to the repository.
+# It runs the `nf-core lint` and markdown lint tests to ensure that the code meets the nf-core guidelines
+on:
+  push:
+  pull_request:
+  release:
+    types: [published]
+
+jobs:
+  Markdown:
+    runs-on: ubuntu-latest
+    steps:
+      - uses: actions/checkout@v2
+      - uses: actions/setup-node@v1
+        with:
+          node-version: '10'
+      - name: Install markdownlint
+        run: npm install -g markdownlint-cli
+      - name: Run Markdownlint
+        run: markdownlint .
+
+      # If the above check failed, post a comment on the PR explaining the failure
+      - name: Post PR comment
+        if: failure()
+        uses: mshick/add-pr-comment@v1
+        with:
+          message: |
+            ## Markdown linting is failing
+
+            To keep the code consistent with lots of contributors, we run automated code consistency checks.
+            To fix this CI test, please run:
+
+            * Install `markdownlint-cli`
+                * On Mac: `brew install markdownlint-cli`
+                * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`)
+            * Fix the markdown errors
+                * Automatically: `markdownlint . --fix`
+                * Manually resolve anything left from `markdownlint .`
+
+            Once you push these changes the test should pass, and you can hide this comment :+1:
+
+            We highly recommend setting up markdownlint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help!
+
+            Thanks again for your contribution!
+          repo-token: ${{ secrets.GITHUB_TOKEN }}
+          allow-repeats: false
+
+  EditorConfig:
+    runs-on: ubuntu-latest
+    steps:
+      - uses: actions/checkout@v2
+
+      - uses: actions/setup-node@v1
+        with:
+          node-version: "10"
+
+      - name: Install editorconfig-checker
+        run: npm install -g editorconfig-checker
+
+      - name: Run ECLint check
+        run: editorconfig-checker -exclude README.md $(git ls-files | grep -v test)
+
+  YAML:
+    runs-on: ubuntu-latest
+    steps:
+      - uses: actions/checkout@v1
+      - uses: actions/setup-node@v1
+        with:
+          node-version: '10'
+      - name: Install yaml-lint
+        run: npm install -g yaml-lint
+      - name: Run yaml-lint
+        run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml" -o -name "*.yaml")
+
+      # If the above check failed, post a comment on the PR explaining the failure
+      - name: Post PR comment
+        if: failure()
+        uses: mshick/add-pr-comment@v1
+        with:
+          message: |
+            ## YAML linting is failing
+
+            To keep the code consistent with lots of contributors, we run automated code consistency checks.
+            To fix this CI test, please run:
+
+            * Install `yaml-lint`
+                * [Install `npm`](https://www.npmjs.com/get-npm) then [install `yaml-lint`](https://www.npmjs.com/package/yaml-lint) (`npm install -g yaml-lint`)
+            * Fix the markdown errors
+                * Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml")`
+                * Fix any reported errors in your YAML files
+
+            Once you push these changes the test should pass, and you can hide this comment :+1:
+
+            We highly recommend setting up yaml-lint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help!
+
+            Thanks again for your contribution!
+          repo-token: ${{ secrets.GITHUB_TOKEN }}
+          allow-repeats: false
+
+  nf-core:
+    runs-on: ubuntu-latest
+    steps:
+
+      - name: Check out pipeline code
+        uses: actions/checkout@v2
+
+      - name: Install Nextflow
+        env:
+          CAPSULE_LOG: none
+        run: |
+          wget -qO- get.nextflow.io | bash
+          sudo mv nextflow /usr/local/bin/
+
+      - uses: actions/setup-python@v1
+        with:
+          python-version: '3.6'
+          architecture: 'x64'
+
+      - name: Install dependencies
+        run: |
+          python -m pip install --upgrade pip
+          pip install nf-core
+
+      - name: Run nf-core lint
+        env:
+          GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+          GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
+        run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
+
+      - name: Save PR number
+        if: ${{ always() }}
+        run: echo ${{ github.event.pull_request.number }} > PR_number.txt
+
+      - name: Upload linting log file artifact
+        if: ${{ always() }}
+        uses: actions/upload-artifact@v2
+        with:
+          name: linting-logs
+          path: |
+            lint_log.txt
+            lint_results.md
+            PR_number.txt
+
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
new file mode 100644
index 00000000..90f03c6f
--- /dev/null
+++ b/.github/workflows/linting_comment.yml
@@ -0,0 +1,29 @@
+
+name: nf-core linting comment
+# This workflow is triggered after the linting action is complete
+# It posts an automated comment to the PR, even if the PR is coming from a fork
+
+on:
+  workflow_run:
+    workflows: ["nf-core linting"]
+
+jobs:
+  test:
+    runs-on: ubuntu-latest
+    steps:
+      - name: Download lint results
+        uses: dawidd6/action-download-artifact@v2
+        with:
+          workflow: linting.yml
+
+      - name: Get PR number
+        id: pr_number
+        run: echo "::set-output name=pr_number::$(cat linting-logs/PR_number.txt)"
+
+      - name: Post PR comment
+        uses: marocchino/sticky-pull-request-comment@v2
+        with:
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+          number: ${{ steps.pr_number.outputs.pr_number }}
+          path: linting-logs/lint_results.md
+
diff --git a/.gitignore b/.gitignore
new file mode 100644
index 00000000..5124c9ac
--- /dev/null
+++ b/.gitignore
@@ -0,0 +1,8 @@
+.nextflow*
+work/
+data/
+results/
+.DS_Store
+testing/
+testing*
+*.pyc
diff --git a/.markdownlint.yml b/.markdownlint.yml
new file mode 100644
index 00000000..9e605fcf
--- /dev/null
+++ b/.markdownlint.yml
@@ -0,0 +1,14 @@
+# Markdownlint configuration file
+default: true
+line-length: false
+ul-indent:
+    indent: 4
+no-duplicate-header:
+    siblings_only: true
+no-inline-html:
+    allowed_elements:
+        - img
+        - p
+        - kbd
+        - details
+        - summary
diff --git a/CHANGELOG.md b/CHANGELOG.md
new file mode 100644
index 00000000..3a8b114f
--- /dev/null
+++ b/CHANGELOG.md
@@ -0,0 +1,16 @@
+# nf-core/rnafusion: Changelog
+
+The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
+and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
+
+## v1.0dev - [date]
+
+Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
+
+### `Added`
+
+### `Fixed`
+
+### `Dependencies`
+
+### `Deprecated`
diff --git a/CITATIONS.md b/CITATIONS.md
new file mode 100644
index 00000000..9b38bf50
--- /dev/null
+++ b/CITATIONS.md
@@ -0,0 +1,32 @@
+# nf-core/rnafusion: Citations
+
+## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/)
+
+> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031.
+
+## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/)
+
+> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311.
+
+## Pipeline tools
+
+* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
+
+* [MultiQC](https://www.ncbi.nlm.nih.gov/pubmed/27312411/)
+    > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
+
+## Software packaging/containerisation tools
+
+* [Anaconda](https://anaconda.com)
+    > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web.
+
+* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/)
+    > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506.
+
+* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/)
+    > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671.
+
+* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241)
+
+* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/)
+    > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675.
diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md
new file mode 100644
index 00000000..f4fd052f
--- /dev/null
+++ b/CODE_OF_CONDUCT.md
@@ -0,0 +1,111 @@
+# Code of Conduct at nf-core (v1.0)
+
+## Our Pledge
+
+In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of:
+
+- Age
+- Body size
+- Familial status
+- Gender identity and expression
+- Geographical location
+- Level of experience
+- Nationality and national origins
+- Native language
+- Physical and neurological ability
+- Race or ethnicity
+- Religion
+- Sexual identity and orientation
+- Socioeconomic status
+
+Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance.
+
+## Preamble
+
+> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply.
+
+An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva.
+
+nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals.
+
+We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc.
+
+Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities.
+
+We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC.
+
+Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re
+
+## Our Responsibilities
+
+The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour.
+
+The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful.
+
+Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC.
+
+## When are where does this Code of Conduct apply?
+
+Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference:
+
+- Communicating with an official project email address.
+- Communicating with community members within the nf-core Slack channel.
+- Participating in hackathons organised by nf-core (both online and in-person events).
+- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence.
+- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc.
+- Representing nf-core on social media. This includes both official and personal accounts.
+
+## nf-core cares 😊
+
+nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order):
+
+- Ask for consent before sharing another community member’s personal information (including photographs) on social media.
+- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity.
+- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !)
+- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.)
+- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can)
+- Focus on what is best for the team and the community. (When in doubt, ask)
+- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn.
+- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!)
+- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**)
+- Take breaks when you feel like you need them.
+- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.)
+
+## nf-core frowns on 😕
+
+The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces.
+
+- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom.
+- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online.
+- Spamming or trolling of individuals on social media.
+- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention.
+- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience.
+
+### Online Trolling
+
+The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately.
+
+All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls.
+
+## Procedures for Reporting CoC violations
+
+If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible.
+
+You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s).
+
+Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course.
+
+All reports will be handled with utmost discretion and confidentially.
+
+## Attribution and Acknowledgements
+
+- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4)
+- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition)
+- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/)
+- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla)
+
+## Changelog
+
+### v1.0 - March 12th, 2021
+
+- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC.
diff --git a/LICENSE b/LICENSE
new file mode 100644
index 00000000..2a63dfa9
--- /dev/null
+++ b/LICENSE
@@ -0,0 +1,21 @@
+MIT License
+
+Copyright (c) @praveenraj2018
+
+Permission is hereby granted, free of charge, to any person obtaining a copy
+of this software and associated documentation files (the "Software"), to deal
+in the Software without restriction, including without limitation the rights
+to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
+copies of the Software, and to permit persons to whom the Software is
+furnished to do so, subject to the following conditions:
+
+The above copyright notice and this permission notice shall be included in all
+copies or substantial portions of the Software.
+
+THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
+IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
+FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
+AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
+LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
+OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
+SOFTWARE.
diff --git a/README.md b/README.md
new file mode 100644
index 00000000..b5340698
--- /dev/null
+++ b/README.md
@@ -0,0 +1,90 @@
+# ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo.png)
+
+[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+CI%22)
+[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)
+[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)
+[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
+
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.04.0-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
+[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
+[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
+[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
+
+[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)
+[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)
+[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
+
+## Introduction
+
+<!-- TODO nf-core: Write a 1-2 sentence summary of what data the pipeline is for and what it does -->
+**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing analysis pipeline with curated list of tools for detecting and visualizing fusion genes..
+
+The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
+
+<!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
+On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).
+
+## Pipeline summary
+
+<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
+
+1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
+
+## Quick Start
+
+1. Install [`Nextflow`](https://nf-co.re/usage/installation) (`>=21.04.0`)
+
+2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
+
+3. Download the pipeline and test it on a minimal dataset with a single command:
+
+    ```console
+    nextflow run nf-core/rnafusion -profile test,<docker/singularity/podman/shifter/charliecloud/conda/institute>
+    ```
+
+    > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
+    > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, it is highly recommended to use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to pre-download all of the required containers before running the pipeline and to set the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options to be able to store and re-use the images from a central location for future pipeline runs.
+    > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
+
+4. Start running your own analysis!
+
+    <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
+
+    ```console
+    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input samplesheet.csv --genome GRCh37
+    ```
+
+## Documentation
+
+The nf-core/rnafusion pipeline comes with documentation about the pipeline [usage](https://nf-co.re/rnafusion/usage), [parameters](https://nf-co.re/rnafusion/parameters) and [output](https://nf-co.re/rnafusion/output).
+
+## Credits
+
+nf-core/rnafusion was originally written by @praveenraj2018.
+
+We thank the following people for their extensive assistance in the development of this pipeline:
+
+<!-- TODO nf-core: If applicable, make list of people who have also contributed -->
+
+## Contributions and Support
+
+If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).
+
+For further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).
+
+## Citations
+
+<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->
+<!-- If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
+
+<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
+An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
+
+You can cite the `nf-core` publication as follows:
+
+> **The nf-core framework for community-curated bioinformatics pipelines.**
+>
+> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.
+>
+> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).
diff --git a/assets/email_template.html b/assets/email_template.html
new file mode 100644
index 00000000..963febb1
--- /dev/null
+++ b/assets/email_template.html
@@ -0,0 +1,53 @@
+<html>
+<head>
+  <meta charset="utf-8">
+  <meta http-equiv="X-UA-Compatible" content="IE=edge">
+  <meta name="viewport" content="width=device-width, initial-scale=1">
+
+  <meta name="description" content="nf-core/rnafusion: RNA sequencing analysis pipeline with curated list of tools for detecting and visualizing fusion genes.">
+  <title>nf-core/rnafusion Pipeline Report</title>
+</head>
+<body>
+<div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto;">
+
+<img src="cid:nfcorepipelinelogo">
+
+<h1>nf-core/rnafusion v${version}</h1>
+<h2>Run Name: $runName</h2>
+
+<% if (!success){
+    out << """
+    <div style="color: #a94442; background-color: #f2dede; border-color: #ebccd1; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
+        <h4 style="margin-top:0; color: inherit;">nf-core/rnafusion execution completed unsuccessfully!</h4>
+        <p>The exit status of the task that caused the workflow execution to fail was: <code>$exitStatus</code>.</p>
+        <p>The full error message was:</p>
+        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0;">${errorReport}</pre>
+    </div>
+    """
+} else {
+    out << """
+    <div style="color: #3c763d; background-color: #dff0d8; border-color: #d6e9c6; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
+        nf-core/rnafusion execution completed successfully!
+    </div>
+    """
+}
+%>
+
+<p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
+<p>The command used to launch the workflow was as follows:</p>
+<pre style="white-space: pre-wrap; overflow: visible; background-color: #ededed; padding: 15px; border-radius: 4px; margin-bottom:30px;">$commandLine</pre>
+
+<h3>Pipeline Configuration:</h3>
+<table style="width:100%; max-width:100%; border-spacing: 0; border-collapse: collapse; border:0; margin-bottom: 30px;">
+    <tbody style="border-bottom: 1px solid #ddd;">
+        <% out << summary.collect{ k,v -> "<tr><th style='text-align:left; padding: 8px 0; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'>$k</th><td style='text-align:left; padding: 8px; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'><pre style='white-space: pre-wrap; overflow: visible;'>$v</pre></td></tr>" }.join("\n") %>
+    </tbody>
+</table>
+
+<p>nf-core/rnafusion</p>
+<p><a href="https://github.com/nf-core/rnafusion">https://github.com/nf-core/rnafusion</a></p>
+
+</div>
+
+</body>
+</html>
diff --git a/assets/email_template.txt b/assets/email_template.txt
new file mode 100644
index 00000000..f717ee9f
--- /dev/null
+++ b/assets/email_template.txt
@@ -0,0 +1,40 @@
+----------------------------------------------------
+                                        ,--./,-.
+        ___     __   __   __   ___     /,-._.--~\\
+  |\\ | |__  __ /  ` /  \\ |__) |__         }  {
+  | \\| |       \\__, \\__/ |  \\ |___     \\`-._,-`-,
+                                        `._,._,'
+  nf-core/rnafusion v${version}
+----------------------------------------------------
+
+Run Name: $runName
+
+<% if (success){
+    out << "## nf-core/rnafusion execution completed successfully! ##"
+} else {
+    out << """####################################################
+## nf-core/rnafusion execution completed unsuccessfully! ##
+####################################################
+The exit status of the task that caused the workflow execution to fail was: $exitStatus.
+The full error message was:
+
+${errorReport}
+"""
+} %>
+
+
+The workflow was completed at $dateComplete (duration: $duration)
+
+The command used to launch the workflow was as follows:
+
+  $commandLine
+
+
+
+Pipeline Configuration:
+-----------------------
+<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %>
+
+--
+nf-core/rnafusion
+https://github.com/nf-core/rnafusion
diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml
new file mode 100644
index 00000000..14f8ada3
--- /dev/null
+++ b/assets/multiqc_config.yaml
@@ -0,0 +1,11 @@
+report_comment: >
+    This report has been generated by the <a href="https://github.com/nf-core/rnafusion" target="_blank">nf-core/rnafusion</a>
+    analysis pipeline. For information about how to interpret these results, please see the
+    <a href="https://github.com/nf-core/rnafusion" target="_blank">documentation</a>.
+report_section_order:
+    software_versions:
+        order: -1000
+    nf-core-rnafusion-summary:
+        order: -1001
+
+export_plots: true
diff --git a/assets/nf-core-rnafusion_logo.png b/assets/nf-core-rnafusion_logo.png
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diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv
new file mode 100644
index 00000000..5f653ab7
--- /dev/null
+++ b/assets/samplesheet.csv
@@ -0,0 +1,3 @@
+sample,fastq_1,fastq_2
+SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz
+SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz,
diff --git a/assets/schema_input.json b/assets/schema_input.json
new file mode 100644
index 00000000..435d174a
--- /dev/null
+++ b/assets/schema_input.json
@@ -0,0 +1,39 @@
+{
+    "$schema": "http://json-schema.org/draft-07/schema",
+    "$id": "https://raw.githubusercontent.com/nf-core/rnafusion/master/assets/schema_input.json",
+    "title": "nf-core/rnafusion pipeline - params.input schema",
+    "description": "Schema for the file provided with params.input",
+    "type": "array",
+    "items": {
+        "type": "object",
+        "properties": {
+            "sample": {
+                "type": "string",
+                "pattern": "^\\S+$",
+                "errorMessage": "Sample name must be provided and cannot contain spaces"
+            },
+            "fastq_1": {
+                "type": "string",
+                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
+                "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
+            },
+            "fastq_2": {
+                "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'",
+                "anyOf": [
+                    {
+                        "type": "string",
+                        "pattern": "^\\S+\\.f(ast)?q\\.gz$"
+                    },
+                    {
+                        "type": "string",
+                        "maxLength": 0
+                    }
+                ]
+            }
+        },
+        "required": [
+            "sample",
+            "fastq_1"
+        ]
+    }
+}
diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt
new file mode 100644
index 00000000..06728832
--- /dev/null
+++ b/assets/sendmail_template.txt
@@ -0,0 +1,53 @@
+To: $email
+Subject: $subject
+Mime-Version: 1.0
+Content-Type: multipart/related;boundary="nfcoremimeboundary"
+
+--nfcoremimeboundary
+Content-Type: text/html; charset=utf-8
+
+$email_html
+
+--nfcoremimeboundary
+Content-Type: image/png;name="nf-core-rnafusion_logo.png"
+Content-Transfer-Encoding: base64
+Content-ID: <nfcorepipelinelogo>
+Content-Disposition: inline; filename="nf-core-rnafusion_logo.png"
+
+<% out << new File("$projectDir/assets/nf-core-rnafusion_logo.png").
+    bytes.
+    encodeBase64().
+    toString().
+    tokenize( '\n' )*.
+    toList()*.
+    collate( 76 )*.
+    collect { it.join() }.
+    flatten().
+    join( '\n' ) %>
+
+<%
+if (mqcFile){
+def mqcFileObj = new File("$mqcFile")
+if (mqcFileObj.length() < mqcMaxSize){
+out << """
+--nfcoremimeboundary
+Content-Type: text/html; name=\"multiqc_report\"
+Content-Transfer-Encoding: base64
+Content-ID: <mqcreport>
+Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\"
+
+${mqcFileObj.
+    bytes.
+    encodeBase64().
+    toString().
+    tokenize( '\n' )*.
+    toList()*.
+    collate( 76 )*.
+    collect { it.join() }.
+    flatten().
+    join( '\n' )}
+"""
+}}
+%>
+
+--nfcoremimeboundary--
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
new file mode 100755
index 00000000..dff05443
--- /dev/null
+++ b/bin/check_samplesheet.py
@@ -0,0 +1,146 @@
+#!/usr/bin/env python
+
+# TODO nf-core: Update the script to check the samplesheet
+# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
+
+import os
+import sys
+import errno
+import argparse
+
+
+def parse_args(args=None):
+    Description = "Reformat nf-core/rnafusion samplesheet file and check its contents."
+    Epilog = "Example usage: python check_samplesheet.py <FILE_IN> <FILE_OUT>"
+
+    parser = argparse.ArgumentParser(description=Description, epilog=Epilog)
+    parser.add_argument("FILE_IN", help="Input samplesheet file.")
+    parser.add_argument("FILE_OUT", help="Output file.")
+    return parser.parse_args(args)
+
+
+def make_dir(path):
+    if len(path) > 0:
+        try:
+            os.makedirs(path)
+        except OSError as exception:
+            if exception.errno != errno.EEXIST:
+                raise exception
+
+
+def print_error(error, context="Line", context_str=""):
+    error_str = "ERROR: Please check samplesheet -> {}".format(error)
+    if context != "" and context_str != "":
+        error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format(
+            error, context.strip(), context_str.strip()
+        )
+    print(error_str)
+    sys.exit(1)
+
+
+# TODO nf-core: Update the check_samplesheet function
+def check_samplesheet(file_in, file_out):
+    """
+    This function checks that the samplesheet follows the following structure:
+
+    sample,fastq_1,fastq_2
+    SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz
+    SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz
+    SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,
+
+    For an example see:
+    https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
+    """
+
+    sample_mapping_dict = {}
+    with open(file_in, "r") as fin:
+
+        ## Check header
+        MIN_COLS = 2
+        # TODO nf-core: Update the column names for the input samplesheet
+        HEADER = ["sample", "fastq_1", "fastq_2"]
+        header = [x.strip('"') for x in fin.readline().strip().split(",")]
+        if header[: len(HEADER)] != HEADER:
+            print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
+            sys.exit(1)
+
+        ## Check sample entries
+        for line in fin:
+            lspl = [x.strip().strip('"') for x in line.strip().split(",")]
+
+            # Check valid number of columns per row
+            if len(lspl) < len(HEADER):
+                print_error(
+                    "Invalid number of columns (minimum = {})!".format(len(HEADER)),
+                    "Line",
+                    line,
+                )
+            num_cols = len([x for x in lspl if x])
+            if num_cols < MIN_COLS:
+                print_error(
+                    "Invalid number of populated columns (minimum = {})!".format(MIN_COLS),
+                    "Line",
+                    line,
+                )
+
+            ## Check sample name entries
+            sample, fastq_1, fastq_2 = lspl[: len(HEADER)]
+            sample = sample.replace(" ", "_")
+            if not sample:
+                print_error("Sample entry has not been specified!", "Line", line)
+
+            ## Check FastQ file extension
+            for fastq in [fastq_1, fastq_2]:
+                if fastq:
+                    if fastq.find(" ") != -1:
+                        print_error("FastQ file contains spaces!", "Line", line)
+                    if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"):
+                        print_error(
+                            "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!",
+                            "Line",
+                            line,
+                        )
+
+            ## Auto-detect paired-end/single-end
+            sample_info = []  ## [single_end, fastq_1, fastq_2]
+            if sample and fastq_1 and fastq_2:  ## Paired-end short reads
+                sample_info = ["0", fastq_1, fastq_2]
+            elif sample and fastq_1 and not fastq_2:  ## Single-end short reads
+                sample_info = ["1", fastq_1, fastq_2]
+            else:
+                print_error("Invalid combination of columns provided!", "Line", line)
+
+            ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] }
+            if sample not in sample_mapping_dict:
+                sample_mapping_dict[sample] = [sample_info]
+            else:
+                if sample_info in sample_mapping_dict[sample]:
+                    print_error("Samplesheet contains duplicate rows!", "Line", line)
+                else:
+                    sample_mapping_dict[sample].append(sample_info)
+
+    ## Write validated samplesheet with appropriate columns
+    if len(sample_mapping_dict) > 0:
+        out_dir = os.path.dirname(file_out)
+        make_dir(out_dir)
+        with open(file_out, "w") as fout:
+            fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n")
+            for sample in sorted(sample_mapping_dict.keys()):
+
+                ## Check that multiple runs of the same sample are of the same datatype
+                if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]):
+                    print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample))
+
+                for idx, val in enumerate(sample_mapping_dict[sample]):
+                    fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n")
+    else:
+        print_error("No entries to process!", "Samplesheet: {}".format(file_in))
+
+
+def main(args=None):
+    args = parse_args(args)
+    check_samplesheet(args.FILE_IN, args.FILE_OUT)
+
+
+if __name__ == "__main__":
+    sys.exit(main())
diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
new file mode 100755
index 00000000..590af3bb
--- /dev/null
+++ b/bin/scrape_software_versions.py
@@ -0,0 +1,36 @@
+#!/usr/bin/env python
+from __future__ import print_function
+import os
+
+results = {}
+version_files = [x for x in os.listdir(".") if x.endswith(".version.txt")]
+for version_file in version_files:
+
+    software = version_file.replace(".version.txt", "")
+    if software == "pipeline":
+        software = "nf-core/rnafusion"
+
+    with open(version_file) as fin:
+        version = fin.read().strip()
+    results[software] = version
+
+# Dump to YAML
+print(
+    """
+id: 'software_versions'
+section_name: 'nf-core/rnafusion Software Versions'
+section_href: 'https://github.com/nf-core/rnafusion'
+plot_type: 'html'
+description: 'are collected at run time from the software output.'
+data: |
+    <dl class="dl-horizontal">
+"""
+)
+for k, v in sorted(results.items()):
+    print("        <dt>{}</dt><dd><samp>{}</samp></dd>".format(k, v))
+print("    </dl>")
+
+# Write out regexes as csv file:
+with open("software_versions.tsv", "w") as f:
+    for k, v in sorted(results.items()):
+        f.write("{}\t{}\n".format(k, v))
diff --git a/conf/base.config b/conf/base.config
new file mode 100644
index 00000000..5619f83a
--- /dev/null
+++ b/conf/base.config
@@ -0,0 +1,57 @@
+/*
+========================================================================================
+    nf-core/rnafusion Nextflow base config file
+========================================================================================
+    A 'blank slate' config file, appropriate for general use on most high performance
+    compute environments. Assumes that all software is installed and available on
+    the PATH. Runs in `local` mode - all jobs will be run on the logged in environment.
+----------------------------------------------------------------------------------------
+*/
+
+process {
+
+    // TODO nf-core: Check the defaults for all processes
+    cpus   = { check_max( 1    * task.attempt, 'cpus'   ) }
+    memory = { check_max( 6.GB * task.attempt, 'memory' ) }
+    time   = { check_max( 4.h  * task.attempt, 'time'   ) }
+
+    errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' }
+    maxRetries    = 1
+    maxErrors     = '-1'
+
+    // Process-specific resource requirements
+    // NOTE - Please try and re-use the labels below as much as possible.
+    //        These labels are used and recognised by default in DSL2 files hosted on nf-core/modules.
+    //        If possible, it would be nice to keep the same label naming convention when
+    //        adding in your local modules too.
+    // TODO nf-core: Customise requirements for specific processes.
+    // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors
+    withLabel:process_low {
+        cpus   = { check_max( 2     * task.attempt, 'cpus'    ) }
+        memory = { check_max( 12.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 4.h   * task.attempt, 'time'    ) }
+    }
+    withLabel:process_medium {
+        cpus   = { check_max( 6     * task.attempt, 'cpus'    ) }
+        memory = { check_max( 36.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 8.h   * task.attempt, 'time'    ) }
+    }
+    withLabel:process_high {
+        cpus   = { check_max( 12    * task.attempt, 'cpus'    ) }
+        memory = { check_max( 72.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 16.h  * task.attempt, 'time'    ) }
+    }
+    withLabel:process_long {
+        time   = { check_max( 20.h  * task.attempt, 'time'    ) }
+    }
+    withLabel:process_high_memory {
+        memory = { check_max( 200.GB * task.attempt, 'memory' ) }
+    }
+    withLabel:error_ignore {
+        errorStrategy = 'ignore'
+    }
+    withLabel:error_retry {
+        errorStrategy = 'retry'
+        maxRetries    = 2
+    }
+}
diff --git a/conf/igenomes.config b/conf/igenomes.config
new file mode 100644
index 00000000..855948de
--- /dev/null
+++ b/conf/igenomes.config
@@ -0,0 +1,432 @@
+/*
+========================================================================================
+    Nextflow config file for iGenomes paths
+========================================================================================
+    Defines reference genomes using iGenome paths.
+    Can be used by any config that customises the base path using:
+        $params.igenomes_base / --igenomes_base
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    // illumina iGenomes reference file paths
+    genomes {
+        'GRCh37' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
+        }
+        'GRCh38' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
+        }
+        'GRCm38' {
+            fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "1.87e9"
+            blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
+        }
+        'TAIR10' {
+            fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
+            mito_name   = "Mt"
+        }
+        'EB2' {
+            fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
+        }
+        'UMD3.1' {
+            fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'WBcel235' {
+            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
+            mito_name   = "MtDNA"
+            macs_gsize  = "9e7"
+        }
+        'CanFam3.1' {
+            fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'GRCz10' {
+            fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'BDGP6' {
+            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
+            mito_name   = "M"
+            macs_gsize  = "1.2e8"
+        }
+        'EquCab2' {
+            fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'EB1' {
+            fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
+        }
+        'Galgal4' {
+            fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'Gm01' {
+            fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
+        }
+        'Mmul_1' {
+            fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'IRGSP-1.0' {
+            fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
+            mito_name   = "Mt"
+        }
+        'CHIMP2.1.4' {
+            fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'Rnor_5.0' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'Rnor_6.0' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'R64-1-1' {
+            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+            macs_gsize  = "1.2e7"
+        }
+        'EF2' {
+            fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "1.21e7"
+        }
+        'Sbi1' {
+            fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
+        }
+        'Sscrofa10.2' {
+            fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'AGPv3' {
+            fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
+            mito_name   = "Mt"
+        }
+        'hg38' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
+        }
+        'hg19' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
+        }
+        'mm10' {
+            fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "1.87e9"
+            blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
+        }
+        'bosTau8' {
+            fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+        }
+        'ce10' {
+            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "9e7"
+        }
+        'canFam3' {
+            fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'danRer10' {
+            fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "1.37e9"
+        }
+        'dm6' {
+            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "1.2e8"
+        }
+        'equCab2' {
+            fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'galGal4' {
+            fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'panTro4' {
+            fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'rn6' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+        }
+        'sacCer3' {
+            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
+            readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "1.2e7"
+        }
+        'susScr3' {
+            fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+    }
+}
diff --git a/conf/modules.config b/conf/modules.config
new file mode 100644
index 00000000..0b1bfdec
--- /dev/null
+++ b/conf/modules.config
@@ -0,0 +1,32 @@
+/*
+========================================================================================
+    Config file for defining DSL2 per module options
+========================================================================================
+    Available keys to override module options:
+        args            = Additional arguments appended to command in module.
+        args2           = Second set of arguments appended to command in module (multi-tool modules).
+        args3           = Third set of arguments appended to command in module (multi-tool modules).
+        publish_dir     = Directory to publish results.
+        publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path
+                            If publish_by_meta = true                 - Value of ${meta['id']} is appended as a directory to "publish_dir" path
+                            If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/"
+                                                                        is appended as a directory to "publish_dir" path
+                            If publish_by_meta = false / null         - No directories are appended to "publish_dir" path
+        publish_files   = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension
+                            The value of "directory" is appended to the standard "publish_dir" path as defined above.
+                            If publish_files = null (unspecified)     - All files are published.
+                            If publish_files = false                  - No files are published.
+        suffix          = File name suffix for output files.
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    modules {
+        'fastqc' {
+            args = "--quiet"
+        }
+        'multiqc' {
+            args = ""
+        }
+    }
+}
diff --git a/conf/test.config b/conf/test.config
new file mode 100644
index 00000000..331ff129
--- /dev/null
+++ b/conf/test.config
@@ -0,0 +1,29 @@
+/*
+========================================================================================
+    Nextflow config file for running minimal tests
+========================================================================================
+    Defines input files and everything required to run a fast and simple pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/rnafusion -profile test,<docker/singularity>
+
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    config_profile_name        = 'Test profile'
+    config_profile_description = 'Minimal test dataset to check pipeline function'
+
+    // Limit resources so that this can run on GitHub Actions
+    max_cpus   = 2
+    max_memory = 6.GB
+    max_time   = 6.h
+
+    // Input data
+    // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
+    // TODO nf-core: Give any required params for the test so that command line flags are not needed
+    input  = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
+
+    // Genome references
+    genome = 'R64-1-1'
+}
diff --git a/conf/test_full.config b/conf/test_full.config
new file mode 100644
index 00000000..260cb93f
--- /dev/null
+++ b/conf/test_full.config
@@ -0,0 +1,24 @@
+/*
+========================================================================================
+    Nextflow config file for running full-size tests
+========================================================================================
+    Defines input files and everything required to run a full size pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/rnafusion -profile test_full,<docker/singularity>
+
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    config_profile_name        = 'Full test profile'
+    config_profile_description = 'Full test dataset to check pipeline function'
+
+    // Input data for full size test
+    // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA)
+    // TODO nf-core: Give any required params for the test so that command line flags are not needed
+    input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv'
+
+    // Genome references
+    genome = 'R64-1-1'
+}
diff --git a/docs/README.md b/docs/README.md
new file mode 100644
index 00000000..08dec85e
--- /dev/null
+++ b/docs/README.md
@@ -0,0 +1,10 @@
+# nf-core/rnafusion: Documentation
+
+The nf-core/rnafusion documentation is split into the following pages:
+
+* [Usage](usage.md)
+    * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
+* [Output](output.md)
+    * An overview of the different results produced by the pipeline and how to interpret them.
+
+You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re)
diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png
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diff --git a/docs/output.md b/docs/output.md
new file mode 100644
index 00000000..8a3fc362
--- /dev/null
+++ b/docs/output.md
@@ -0,0 +1,68 @@
+# nf-core/rnafusion: Output
+
+## Introduction
+
+This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline.
+
+The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory.
+
+<!-- TODO nf-core: Write this documentation describing your workflow's output -->
+
+## Pipeline overview
+
+The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
+
+* [FastQC](#fastqc) - Raw read QC
+* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
+* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
+
+### FastQC
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `fastqc/`
+    * `*_fastqc.html`: FastQC report containing quality metrics.
+    * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
+
+</details>
+
+[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
+
+![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png)
+
+![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png)
+
+![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png)
+
+> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
+
+### MultiQC
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `multiqc/`
+    * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
+    * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
+    * `multiqc_plots/`: directory containing static images from the report in various formats.
+
+</details>
+
+[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.
+
+Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.
+
+### Pipeline information
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `pipeline_info/`
+    * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
+    * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.tsv`.
+    * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
+
+</details>
+
+[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
diff --git a/docs/usage.md b/docs/usage.md
new file mode 100644
index 00000000..7f14f65b
--- /dev/null
+++ b/docs/usage.md
@@ -0,0 +1,284 @@
+# nf-core/rnafusion: Usage
+
+## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/rnafusion/usage](https://nf-co.re/rnafusion/usage)
+
+> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._
+
+## Introduction
+
+<!-- TODO nf-core: Add documentation about anything specific to running your pipeline. For general topics, please point to (and add to) the main nf-core website. -->
+
+## Samplesheet input
+
+You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below.
+
+```console
+--input '[path to samplesheet file]'
+```
+
+### Multiple runs of the same sample
+
+The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes:
+
+```console
+sample,fastq_1,fastq_2
+CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
+CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz
+CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz
+```
+
+### Full samplesheet
+
+The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below.
+
+A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice.
+
+```console
+sample,fastq_1,fastq_2
+CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
+CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz
+CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz
+TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz,
+TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz,
+TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,
+TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,
+```
+
+| Column         | Description                                                                                                                                                                            |
+|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
+| `sample`       | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). |
+| `fastq_1`      | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
+| `fastq_2`      | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
+
+An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline.
+
+## Running the pipeline
+
+The typical command for running the pipeline is as follows:
+
+```console
+nextflow run nf-core/rnafusion --input samplesheet.csv --genome GRCh37 -profile docker
+```
+
+This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
+
+Note that the pipeline will create the following files in your working directory:
+
+```console
+work            # Directory containing the nextflow working files
+results         # Finished results (configurable, see below)
+.nextflow_log   # Log file from Nextflow
+# Other nextflow hidden files, eg. history of pipeline runs and old logs.
+```
+
+### Updating the pipeline
+
+When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:
+
+```console
+nextflow pull nf-core/rnafusion
+```
+
+### Reproducibility
+
+It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since.
+
+First, go to the [nf-core/rnafusion releases page](https://github.com/nf-core/rnafusion/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`.
+
+This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future.
+
+## Core Nextflow arguments
+
+> **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen).
+
+### `-profile`
+
+Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments.
+
+Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. When using Biocontainers, most of these software packaging methods pull Docker containers from quay.io e.g [FastQC](https://quay.io/repository/biocontainers/fastqc) except for Singularity which directly downloads Singularity images via https hosted by the [Galaxy project](https://depot.galaxyproject.org/singularity/) and Conda which downloads and installs software locally from [Bioconda](https://bioconda.github.io/).
+
+> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
+
+The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation).
+
+Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important!
+They are loaded in sequence, so later profiles can overwrite earlier profiles.
+
+If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended.
+
+* `docker`
+    * A generic configuration profile to be used with [Docker](https://docker.com/)
+* `singularity`
+    * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
+* `podman`
+    * A generic configuration profile to be used with [Podman](https://podman.io/)
+* `shifter`
+    * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
+* `charliecloud`
+    * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
+* `conda`
+    * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
+* `test`
+    * A profile with a complete configuration for automated testing
+    * Includes links to test data so needs no other parameters
+
+### `-resume`
+
+Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously.
+
+You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names.
+
+### `-c`
+
+Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information.
+
+## Custom configuration
+
+### Resource requests
+
+Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped.
+
+For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue:
+
+```console
+[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
+Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
+
+Caused by:
+    Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
+
+Command executed:
+    STAR \
+        --genomeDir star \
+        --readFilesIn WT_REP1_trimmed.fq.gz  \
+        --runThreadN 2 \
+        --outFileNamePrefix WT_REP1. \
+        <TRUNCATED>
+
+Command exit status:
+    137
+
+Command output:
+    (empty)
+
+Command error:
+    .command.sh: line 9:  30 Killed    STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. <TRUNCATED>
+Work dir:
+    /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb
+
+Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
+```
+
+To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
+
+```nextflow
+process {
+    withName: STAR_ALIGN {
+        memory = 100.GB
+    }
+}
+```
+
+> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow.
+
+### Tool-specific options
+
+For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes.
+
+The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of:
+
+* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file.
+
+* An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option.
+
+The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments.
+
+When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`.
+
+As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file.
+
+Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config.
+
+As you will see in the example below, we have:
+
+* appended `--outFilterMismatchNmax 16` to the default `args` used by the module.
+* changed the default `publish_dir` value to where the files will eventually be published in the main results directory.
+* appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`.
+
+```nextflow
+params {
+    modules {
+        'star_align' {
+            args          = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16"
+            publish_dir   = "my_star_directory"
+            publish_files = ['out':'log', 'tab':'log', 'bam':'']
+        }
+    }
+}
+```
+
+### Updating containers
+
+The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`.
+
+1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19)
+2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
+3. Create the custom config accordingly:
+
+    * For Docker:
+
+        ```nextflow
+        process {
+            withName: PANGOLIN {
+                container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0'
+            }
+        }
+        ```
+
+    * For Singularity:
+
+        ```nextflow
+        process {
+            withName: PANGOLIN {
+                container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0'
+            }
+        }
+        ```
+
+    * For Conda:
+
+        ```nextflow
+        process {
+            withName: PANGOLIN {
+                conda = 'bioconda::pangolin=3.0.5'
+            }
+        }
+        ```
+
+> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch.
+
+### nf-core/configs
+
+In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile.
+
+See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files.
+
+If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs).
+
+## Running in the background
+
+Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished.
+
+The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file.
+
+Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time.
+Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs).
+
+## Nextflow memory requirements
+
+In some cases, the Nextflow Java virtual machines can start to request a large amount of memory.
+We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`):
+
+```console
+NXF_OPTS='-Xms1g -Xmx4g'
+```
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
new file mode 100755
index 00000000..8d6920dd
--- /dev/null
+++ b/lib/NfcoreSchema.groovy
@@ -0,0 +1,517 @@
+//
+// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template.
+//
+
+import org.everit.json.schema.Schema
+import org.everit.json.schema.loader.SchemaLoader
+import org.everit.json.schema.ValidationException
+import org.json.JSONObject
+import org.json.JSONTokener
+import org.json.JSONArray
+import groovy.json.JsonSlurper
+import groovy.json.JsonBuilder
+
+class NfcoreSchema {
+
+    //
+    // Resolve Schema path relative to main workflow directory
+    //
+    public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') {
+        return "${workflow.projectDir}/${schema_filename}"
+    }
+
+    //
+    // Function to loop over all parameters defined in schema and check
+    // whether the given parameters adhere to the specifications
+    //
+    /* groovylint-disable-next-line UnusedPrivateMethodParameter */
+    public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') {
+        def has_error = false
+        //=====================================================================//
+        // Check for nextflow core params and unexpected params
+        def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text
+        def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
+        def nf_params = [
+            // Options for base `nextflow` command
+            'bg',
+            'c',
+            'C',
+            'config',
+            'd',
+            'D',
+            'dockerize',
+            'h',
+            'log',
+            'q',
+            'quiet',
+            'syslog',
+            'v',
+            'version',
+
+            // Options for `nextflow run` command
+            'ansi',
+            'ansi-log',
+            'bg',
+            'bucket-dir',
+            'c',
+            'cache',
+            'config',
+            'dsl2',
+            'dump-channels',
+            'dump-hashes',
+            'E',
+            'entry',
+            'latest',
+            'lib',
+            'main-script',
+            'N',
+            'name',
+            'offline',
+            'params-file',
+            'pi',
+            'plugins',
+            'poll-interval',
+            'pool-size',
+            'profile',
+            'ps',
+            'qs',
+            'queue-size',
+            'r',
+            'resume',
+            'revision',
+            'stdin',
+            'stub',
+            'stub-run',
+            'test',
+            'w',
+            'with-charliecloud',
+            'with-conda',
+            'with-dag',
+            'with-docker',
+            'with-mpi',
+            'with-notification',
+            'with-podman',
+            'with-report',
+            'with-singularity',
+            'with-timeline',
+            'with-tower',
+            'with-trace',
+            'with-weblog',
+            'without-docker',
+            'without-podman',
+            'work-dir'
+        ]
+        def unexpectedParams = []
+
+        // Collect expected parameters from the schema
+        def expectedParams = []
+        for (group in schemaParams) {
+            for (p in group.value['properties']) {
+                expectedParams.push(p.key)
+            }
+        }
+
+        for (specifiedParam in params.keySet()) {
+            // nextflow params
+            if (nf_params.contains(specifiedParam)) {
+                log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'"
+                has_error = true
+            }
+            // unexpected params
+            def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params'
+            def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() }
+            def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase()
+            def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase))
+            if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) {
+                // Temporarily remove camelCase/camel-case params #1035
+                def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()}
+                if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){
+                    unexpectedParams.push(specifiedParam)
+                }
+            }
+        }
+
+        //=====================================================================//
+        // Validate parameters against the schema
+        InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream()
+        JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream))
+
+        // Remove anything that's in params.schema_ignore_params
+        raw_schema = removeIgnoredParams(raw_schema, params)
+
+        Schema schema = SchemaLoader.load(raw_schema)
+
+        // Clean the parameters
+        def cleanedParams = cleanParameters(params)
+
+        // Convert to JSONObject
+        def jsonParams = new JsonBuilder(cleanedParams)
+        JSONObject params_json = new JSONObject(jsonParams.toString())
+
+        // Validate
+        try {
+            schema.validate(params_json)
+        } catch (ValidationException e) {
+            println ''
+            log.error 'ERROR: Validation of pipeline parameters failed!'
+            JSONObject exceptionJSON = e.toJSON()
+            printExceptions(exceptionJSON, params_json, log)
+            println ''
+            has_error = true
+        }
+
+        // Check for unexpected parameters
+        if (unexpectedParams.size() > 0) {
+            Map colors = NfcoreTemplate.logColours(params.monochrome_logs)
+            println ''
+            def warn_msg = 'Found unexpected parameters:'
+            for (unexpectedParam in unexpectedParams) {
+                warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}"
+            }
+            log.warn warn_msg
+            log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}"
+            println ''
+        }
+
+        if (has_error) {
+            System.exit(1)
+        }
+    }
+
+    //
+    // Beautify parameters for --help
+    //
+    public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') {
+        Map colors = NfcoreTemplate.logColours(params.monochrome_logs)
+        Integer num_hidden = 0
+        String output  = ''
+        output        += 'Typical pipeline command:\n\n'
+        output        += "  ${colors.cyan}${command}${colors.reset}\n\n"
+        Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename))
+        Integer max_chars  = paramsMaxChars(params_map) + 1
+        Integer desc_indent = max_chars + 14
+        Integer dec_linewidth = 160 - desc_indent
+        for (group in params_map.keySet()) {
+            Integer num_params = 0
+            String group_output = colors.underlined + colors.bold + group + colors.reset + '\n'
+            def group_params = params_map.get(group)  // This gets the parameters of that particular group
+            for (param in group_params.keySet()) {
+                if (group_params.get(param).hidden && !params.show_hidden_params) {
+                    num_hidden += 1
+                    continue;
+                }
+                def type = '[' + group_params.get(param).type + ']'
+                def description = group_params.get(param).description
+                def defaultValue = group_params.get(param).default ? " [default: " + group_params.get(param).default.toString() + "]" : ''
+                def description_default = description + colors.dim + defaultValue + colors.reset
+                // Wrap long description texts
+                // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap
+                if (description_default.length() > dec_linewidth){
+                    List olines = []
+                    String oline = "" // " " * indent
+                    description_default.split(" ").each() { wrd ->
+                        if ((oline.size() + wrd.size()) <= dec_linewidth) {
+                            oline += wrd + " "
+                        } else {
+                            olines += oline
+                            oline = wrd + " "
+                        }
+                    }
+                    olines += oline
+                    description_default = olines.join("\n" + " " * desc_indent)
+                }
+                group_output += "  --" +  param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n'
+                num_params += 1
+            }
+            group_output += '\n'
+            if (num_params > 0){
+                output += group_output
+            }
+        }
+        if (num_hidden > 0){
+            output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset
+        }
+        output += NfcoreTemplate.dashedLine(params.monochrome_logs)
+        return output
+    }
+
+    //
+    // Groovy Map summarising parameters/workflow options used by the pipeline
+    //
+    public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') {
+        // Get a selection of core Nextflow workflow options
+        def Map workflow_summary = [:]
+        if (workflow.revision) {
+            workflow_summary['revision'] = workflow.revision
+        }
+        workflow_summary['runName']      = workflow.runName
+        if (workflow.containerEngine) {
+            workflow_summary['containerEngine'] = workflow.containerEngine
+        }
+        if (workflow.container) {
+            workflow_summary['container'] = workflow.container
+        }
+        workflow_summary['launchDir']    = workflow.launchDir
+        workflow_summary['workDir']      = workflow.workDir
+        workflow_summary['projectDir']   = workflow.projectDir
+        workflow_summary['userName']     = workflow.userName
+        workflow_summary['profile']      = workflow.profile
+        workflow_summary['configFiles']  = workflow.configFiles.join(', ')
+
+        // Get pipeline parameters defined in JSON Schema
+        def Map params_summary = [:]
+        def blacklist  = ['hostnames']
+        def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename))
+        for (group in params_map.keySet()) {
+            def sub_params = new LinkedHashMap()
+            def group_params = params_map.get(group)  // This gets the parameters of that particular group
+            for (param in group_params.keySet()) {
+                if (params.containsKey(param) && !blacklist.contains(param)) {
+                    def params_value = params.get(param)
+                    def schema_value = group_params.get(param).default
+                    def param_type   = group_params.get(param).type
+                    if (schema_value != null) {
+                        if (param_type == 'string') {
+                            if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) {
+                                def sub_string = schema_value.replace('\$projectDir', '')
+                                sub_string     = sub_string.replace('\${projectDir}', '')
+                                if (params_value.contains(sub_string)) {
+                                    schema_value = params_value
+                                }
+                            }
+                            if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) {
+                                def sub_string = schema_value.replace('\$params.outdir', '')
+                                sub_string     = sub_string.replace('\${params.outdir}', '')
+                                if ("${params.outdir}${sub_string}" == params_value) {
+                                    schema_value = params_value
+                                }
+                            }
+                        }
+                    }
+
+                    // We have a default in the schema, and this isn't it
+                    if (schema_value != null && params_value != schema_value) {
+                        sub_params.put(param, params_value)
+                    }
+                    // No default in the schema, and this isn't empty
+                    else if (schema_value == null && params_value != "" && params_value != null && params_value != false) {
+                        sub_params.put(param, params_value)
+                    }
+                }
+            }
+            params_summary.put(group, sub_params)
+        }
+        return [ 'Core Nextflow options' : workflow_summary ] << params_summary
+    }
+
+    //
+    // Beautify parameters for summary and return as string
+    //
+    public static String paramsSummaryLog(workflow, params) {
+        Map colors = NfcoreTemplate.logColours(params.monochrome_logs)
+        String output  = ''
+        def params_map = paramsSummaryMap(workflow, params)
+        def max_chars  = paramsMaxChars(params_map)
+        for (group in params_map.keySet()) {
+            def group_params = params_map.get(group)  // This gets the parameters of that particular group
+            if (group_params) {
+                output += colors.bold + group + colors.reset + '\n'
+                for (param in group_params.keySet()) {
+                    output += "  " + colors.blue + param.padRight(max_chars) + ": " + colors.green +  group_params.get(param) + colors.reset + '\n'
+                }
+                output += '\n'
+            }
+        }
+        output += "!! Only displaying parameters that differ from the pipeline defaults !!\n"
+        output += NfcoreTemplate.dashedLine(params.monochrome_logs)
+        return output
+    }
+
+    //
+    // Loop over nested exceptions and print the causingException
+    //
+    private static void printExceptions(ex_json, params_json, log) {
+        def causingExceptions = ex_json['causingExceptions']
+        if (causingExceptions.length() == 0) {
+            def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/
+            // Missing required param
+            if (m.matches()) {
+                log.error "* Missing required parameter: --${m[0][1]}"
+            }
+            // Other base-level error
+            else if (ex_json['pointerToViolation'] == '#') {
+                log.error "* ${ex_json['message']}"
+            }
+            // Error with specific param
+            else {
+                def param = ex_json['pointerToViolation'] - ~/^#\//
+                def param_val = params_json[param].toString()
+                log.error "* --${param}: ${ex_json['message']} (${param_val})"
+            }
+        }
+        for (ex in causingExceptions) {
+            printExceptions(ex, params_json, log)
+        }
+    }
+
+    //
+    // Remove an element from a JSONArray
+    //
+    private static JSONArray removeElement(json_array, element) {
+        def list = []
+        int len = json_array.length()
+        for (int i=0;i<len;i++){
+            list.add(json_array.get(i).toString())
+        }
+        list.remove(element)
+        JSONArray jsArray = new JSONArray(list)
+        return jsArray
+    }
+
+    //
+    // Remove ignored parameters
+    //
+    private static JSONObject removeIgnoredParams(raw_schema, params) {
+        // Remove anything that's in params.schema_ignore_params
+        params.schema_ignore_params.split(',').each{ ignore_param ->
+            if(raw_schema.keySet().contains('definitions')){
+                raw_schema.definitions.each { definition ->
+                    for (key in definition.keySet()){
+                        if (definition[key].get("properties").keySet().contains(ignore_param)){
+                            // Remove the param to ignore
+                            definition[key].get("properties").remove(ignore_param)
+                            // If the param was required, change this
+                            if (definition[key].has("required")) {
+                                def cleaned_required = removeElement(definition[key].required, ignore_param)
+                                definition[key].put("required", cleaned_required)
+                            }
+                        }
+                    }
+                }
+            }
+            if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) {
+                raw_schema.get("properties").remove(ignore_param)
+            }
+            if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) {
+                def cleaned_required = removeElement(raw_schema.required, ignore_param)
+                raw_schema.put("required", cleaned_required)
+            }
+        }
+        return raw_schema
+    }
+
+    //
+    // Clean and check parameters relative to Nextflow native classes
+    //
+    private static Map cleanParameters(params) {
+        def new_params = params.getClass().newInstance(params)
+        for (p in params) {
+            // remove anything evaluating to false
+            if (!p['value']) {
+                new_params.remove(p.key)
+            }
+            // Cast MemoryUnit to String
+            if (p['value'].getClass() == nextflow.util.MemoryUnit) {
+                new_params.replace(p.key, p['value'].toString())
+            }
+            // Cast Duration to String
+            if (p['value'].getClass() == nextflow.util.Duration) {
+                new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day"))
+            }
+            // Cast LinkedHashMap to String
+            if (p['value'].getClass() == LinkedHashMap) {
+                new_params.replace(p.key, p['value'].toString())
+            }
+        }
+        return new_params
+    }
+
+    //
+    // This function tries to read a JSON params file
+    //
+    private static LinkedHashMap paramsLoad(String json_schema) {
+        def params_map = new LinkedHashMap()
+        try {
+            params_map = paramsRead(json_schema)
+        } catch (Exception e) {
+            println "Could not read parameters settings from JSON. $e"
+            params_map = new LinkedHashMap()
+        }
+        return params_map
+    }
+
+    //
+    // Method to actually read in JSON file using Groovy.
+    // Group (as Key), values are all parameters
+    //    - Parameter1 as Key, Description as Value
+    //    - Parameter2 as Key, Description as Value
+    //    ....
+    // Group
+    //    -
+    private static LinkedHashMap paramsRead(String json_schema) throws Exception {
+        def json = new File(json_schema).text
+        def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions')
+        def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties')
+        /* Tree looks like this in nf-core schema
+        * definitions <- this is what the first get('definitions') gets us
+                group 1
+                    title
+                    description
+                        properties
+                        parameter 1
+                            type
+                            description
+                        parameter 2
+                            type
+                            description
+                group 2
+                    title
+                    description
+                        properties
+                        parameter 1
+                            type
+                            description
+        * properties <- parameters can also be ungrouped, outside of definitions
+                parameter 1
+                    type
+                    description
+        */
+
+        // Grouped params
+        def params_map = new LinkedHashMap()
+        schema_definitions.each { key, val ->
+            def Map group = schema_definitions."$key".properties // Gets the property object of the group
+            def title = schema_definitions."$key".title
+            def sub_params = new LinkedHashMap()
+            group.each { innerkey, value ->
+                sub_params.put(innerkey, value)
+            }
+            params_map.put(title, sub_params)
+        }
+
+        // Ungrouped params
+        def ungrouped_params = new LinkedHashMap()
+        schema_properties.each { innerkey, value ->
+            ungrouped_params.put(innerkey, value)
+        }
+        params_map.put("Other parameters", ungrouped_params)
+
+        return params_map
+    }
+
+    //
+    // Get maximum number of characters across all parameter names
+    //
+    private static Integer paramsMaxChars(params_map) {
+        Integer max_chars = 0
+        for (group in params_map.keySet()) {
+            def group_params = params_map.get(group)  // This gets the parameters of that particular group
+            for (param in group_params.keySet()) {
+                if (param.size() > max_chars) {
+                    max_chars = param.size()
+                }
+            }
+        }
+        return max_chars
+    }
+}
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
new file mode 100755
index 00000000..b6e689ec
--- /dev/null
+++ b/lib/NfcoreTemplate.groovy
@@ -0,0 +1,266 @@
+//
+// This file holds several functions used within the nf-core pipeline template.
+//
+
+import org.yaml.snakeyaml.Yaml
+
+class NfcoreTemplate {
+
+    //
+    // Check AWS Batch related parameters have been specified correctly
+    //
+    public static void awsBatch(workflow, params) {
+        if (workflow.profile.contains('awsbatch')) {
+            // Check params.awsqueue and params.awsregion have been set if running on AWSBatch
+            assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!"
+            // Check outdir paths to be S3 buckets if running on AWSBatch
+            assert params.outdir.startsWith('s3:')       : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!"
+        }
+    }
+
+    //
+    // Check params.hostnames
+    //
+    public static void hostName(workflow, params, log) {
+        Map colors = logColours(params.monochrome_logs)
+        if (params.hostnames) {
+            def hostname = "hostname".execute().text.trim()
+            params.hostnames.each { prof, hnames ->
+                hnames.each { hname ->
+                    if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
+                        log.info "=${colors.yellow}====================================================${colors.reset}=\n" +
+                            "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" +
+                            "      but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" +
+                            "      ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" +
+                            "=${colors.yellow}====================================================${colors.reset}="
+                    }
+                }
+            }
+        }
+    }
+
+    //
+    // Construct and send completion email
+    //
+    public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) {
+
+        // Set up the e-mail variables
+        def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
+        if (!workflow.success) {
+            subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
+        }
+
+        def summary = [:]
+        for (group in summary_params.keySet()) {
+            summary << summary_params[group]
+        }
+
+        def misc_fields = [:]
+        misc_fields['Date Started']              = workflow.start
+        misc_fields['Date Completed']            = workflow.complete
+        misc_fields['Pipeline script file path'] = workflow.scriptFile
+        misc_fields['Pipeline script hash ID']   = workflow.scriptId
+        if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
+        if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
+        if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
+        misc_fields['Nextflow Version']           = workflow.nextflow.version
+        misc_fields['Nextflow Build']             = workflow.nextflow.build
+        misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
+
+        def email_fields = [:]
+        email_fields['version']      = workflow.manifest.version
+        email_fields['runName']      = workflow.runName
+        email_fields['success']      = workflow.success
+        email_fields['dateComplete'] = workflow.complete
+        email_fields['duration']     = workflow.duration
+        email_fields['exitStatus']   = workflow.exitStatus
+        email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+        email_fields['errorReport']  = (workflow.errorReport ?: 'None')
+        email_fields['commandLine']  = workflow.commandLine
+        email_fields['projectDir']   = workflow.projectDir
+        email_fields['summary']      = summary << misc_fields
+
+        // On success try attach the multiqc report
+        def mqc_report = null
+        try {
+            if (workflow.success) {
+                mqc_report = multiqc_report.getVal()
+                if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
+                    if (mqc_report.size() > 1) {
+                        log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
+                    }
+                    mqc_report = mqc_report[0]
+                }
+            }
+        } catch (all) {
+            if (multiqc_report) {
+                log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
+            }
+        }
+
+        // Check if we are only sending emails on failure
+        def email_address = params.email
+        if (!params.email && params.email_on_fail && !workflow.success) {
+            email_address = params.email_on_fail
+        }
+
+        // Render the TXT template
+        def engine       = new groovy.text.GStringTemplateEngine()
+        def tf           = new File("$projectDir/assets/email_template.txt")
+        def txt_template = engine.createTemplate(tf).make(email_fields)
+        def email_txt    = txt_template.toString()
+
+        // Render the HTML template
+        def hf            = new File("$projectDir/assets/email_template.html")
+        def html_template = engine.createTemplate(hf).make(email_fields)
+        def email_html    = html_template.toString()
+
+        // Render the sendmail template
+        def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit
+        def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
+        def sf                     = new File("$projectDir/assets/sendmail_template.txt")
+        def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
+        def sendmail_html          = sendmail_template.toString()
+
+        // Send the HTML e-mail
+        Map colors = logColours(params.monochrome_logs)
+        if (email_address) {
+            try {
+                if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
+                // Try to send HTML e-mail using sendmail
+                [ 'sendmail', '-t' ].execute() << sendmail_html
+                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
+            } catch (all) {
+                // Catch failures and try with plaintext
+                def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
+                if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
+                    mail_cmd += [ '-A', mqc_report ]
+                }
+                mail_cmd.execute() << email_html
+                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
+            }
+        }
+
+        // Write summary e-mail HTML to a file
+        def output_d = new File("${params.outdir}/pipeline_info/")
+        if (!output_d.exists()) {
+            output_d.mkdirs()
+        }
+        def output_hf = new File(output_d, "pipeline_report.html")
+        output_hf.withWriter { w -> w << email_html }
+        def output_tf = new File(output_d, "pipeline_report.txt")
+        output_tf.withWriter { w -> w << email_txt }
+    }
+
+    //
+    // Print pipeline summary on completion
+    //
+    public static void summary(workflow, params, log) {
+        Map colors = logColours(params.monochrome_logs)
+        if (workflow.success) {
+            if (workflow.stats.ignoredCount == 0) {
+                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
+            } else {
+                log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
+            }
+        } else {
+            hostName(workflow, params, log)
+            log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
+        }
+    }
+
+    //
+    // ANSII Colours used for terminal logging
+    //
+    public static Map logColours(Boolean monochrome_logs) {
+        Map colorcodes = [:]
+
+        // Reset / Meta
+        colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
+        colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
+        colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
+        colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
+        colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
+        colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
+        colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
+
+        // Regular Colors
+        colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
+        colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
+        colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
+        colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
+        colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
+        colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
+        colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
+        colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
+
+        // Bold
+        colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
+        colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
+        colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
+        colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
+        colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
+        colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
+        colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
+        colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
+
+        // Underline
+        colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
+        colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
+        colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
+        colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
+        colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
+        colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
+        colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
+        colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
+
+        // High Intensity
+        colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
+        colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
+        colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
+        colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
+        colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
+        colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
+        colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
+        colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
+
+        // Bold High Intensity
+        colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
+        colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
+        colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
+        colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
+        colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
+        colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
+        colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
+        colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
+
+        return colorcodes
+    }
+
+    //
+    // Does what is says on the tin
+    //
+    public static String dashedLine(monochrome_logs) {
+        Map colors = logColours(monochrome_logs)
+        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
+    }
+
+    //
+    // nf-core logo
+    //
+    public static String logo(workflow, monochrome_logs) {
+        Map colors = logColours(monochrome_logs)
+        String.format(
+            """\n
+            ${dashedLine(monochrome_logs)}
+                                                    ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
+            ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
+            ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
+            ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
+                                                    ${colors.green}`._,._,\'${colors.reset}
+            ${colors.purple}  ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset}
+            ${dashedLine(monochrome_logs)}
+            """.stripIndent()
+        )
+    }
+}
diff --git a/lib/Utils.groovy b/lib/Utils.groovy
new file mode 100755
index 00000000..18173e98
--- /dev/null
+++ b/lib/Utils.groovy
@@ -0,0 +1,47 @@
+//
+// This file holds several Groovy functions that could be useful for any Nextflow pipeline
+//
+
+import org.yaml.snakeyaml.Yaml
+
+class Utils {
+
+    //
+    // When running with -profile conda, warn if channels have not been set-up appropriately
+    //
+    public static void checkCondaChannels(log) {
+        Yaml parser = new Yaml()
+        def channels = []
+        try {
+            def config = parser.load("conda config --show channels".execute().text)
+            channels = config.channels
+        } catch(NullPointerException | IOException e) {
+            log.warn "Could not verify conda channel configuration."
+            return
+        }
+
+        // Check that all channels are present
+        def required_channels = ['conda-forge', 'bioconda', 'defaults']
+        def conda_check_failed = !required_channels.every { ch -> ch in channels }
+
+        // Check that they are in the right order
+        conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda'))
+        conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults'))
+
+        if (conda_check_failed) {
+            log.warn "=============================================================================\n" +
+                "  There is a problem with your Conda configuration!\n\n" +
+                "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
+                "  Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" +
+                "  NB: The order of the channels matters!\n" +
+                "==================================================================================="
+        }
+    }
+
+    //
+    // Join module args with appropriate spacing
+    //
+    public static String joinModuleArgs(args_list) {
+        return ' ' + args_list.join(' ')
+    }
+}
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
new file mode 100755
index 00000000..4caed266
--- /dev/null
+++ b/lib/WorkflowMain.groovy
@@ -0,0 +1,94 @@
+//
+// This file holds several functions specific to the main.nf workflow in the nf-core/rnafusion pipeline
+//
+
+class WorkflowMain {
+
+    //
+    // Citation string for pipeline
+    //
+    public static String citation(workflow) {
+        return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
+            // TODO nf-core: Add Zenodo DOI for pipeline after first release
+            //"* The pipeline\n" +
+            //"  https://doi.org/10.5281/zenodo.XXXXXXX\n\n" +
+            "* The nf-core framework\n" +
+            "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
+            "* Software dependencies\n" +
+            "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
+    }
+
+    //
+    // Print help to screen if required
+    //
+    public static String help(workflow, params, log) {
+        def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker"
+        def help_string = ''
+        help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs)
+        help_string += NfcoreSchema.paramsHelp(workflow, params, command)
+        help_string += '\n' + citation(workflow) + '\n'
+        help_string += NfcoreTemplate.dashedLine(params.monochrome_logs)
+        return help_string
+    }
+
+    //
+    // Print parameter summary log to screen
+    //
+    public static String paramsSummaryLog(workflow, params, log) {
+        def summary_log = ''
+        summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs)
+        summary_log += NfcoreSchema.paramsSummaryLog(workflow, params)
+        summary_log += '\n' + citation(workflow) + '\n'
+        summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs)
+        return summary_log
+    }
+
+    //
+    // Validate parameters and print summary to screen
+    //
+    public static void initialise(workflow, params, log) {
+        // Print help to screen if required
+        if (params.help) {
+            log.info help(workflow, params, log)
+            System.exit(0)
+        }
+
+        // Validate workflow parameters via the JSON schema
+        if (params.validate_params) {
+            NfcoreSchema.validateParameters(workflow, params, log)
+        }
+
+        // Print parameter summary log to screen
+        log.info paramsSummaryLog(workflow, params, log)
+
+        // Check that conda channels are set-up correctly
+        if (params.enable_conda) {
+            Utils.checkCondaChannels(log)
+        }
+
+        // Check AWS batch settings
+        NfcoreTemplate.awsBatch(workflow, params)
+
+        // Check the hostnames against configured profiles
+        NfcoreTemplate.hostName(workflow, params, log)
+
+        // Check input has been provided
+        if (!params.input) {
+            log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'"
+            System.exit(1)
+        }
+    }
+
+    //
+    // Get attribute from genome config file e.g. fasta
+    //
+    public static String getGenomeAttribute(params, attribute) {
+        def val = ''
+        if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) {
+            if (params.genomes[ params.genome ].containsKey(attribute)) {
+                val = params.genomes[ params.genome ][ attribute ]
+            }
+        }
+        return val
+    }
+}
diff --git a/lib/WorkflowRnafusion.groovy b/lib/WorkflowRnafusion.groovy
new file mode 100755
index 00000000..e9d53d8a
--- /dev/null
+++ b/lib/WorkflowRnafusion.groovy
@@ -0,0 +1,59 @@
+//
+// This file holds several functions specific to the workflow/rnafusion.nf in the nf-core/rnafusion pipeline
+//
+
+class WorkflowRnafusion {
+
+    //
+    // Check and validate parameters
+    //
+    public static void initialise(params, log) {
+        genomeExistsError(params, log)
+
+        if (!params.fasta) {
+            log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
+            System.exit(1)
+        }
+    }
+
+    //
+    // Get workflow summary for MultiQC
+    //
+    public static String paramsSummaryMultiqc(workflow, summary) {
+        String summary_section = ''
+        for (group in summary.keySet()) {
+            def group_params = summary.get(group)  // This gets the parameters of that particular group
+            if (group_params) {
+                summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
+                summary_section += "    <dl class=\"dl-horizontal\">\n"
+                for (param in group_params.keySet()) {
+                    summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
+                }
+                summary_section += "    </dl>\n"
+            }
+        }
+
+        String yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n"
+        yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
+        yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
+        yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
+        yaml_file_text        += "plot_type: 'html'\n"
+        yaml_file_text        += "data: |\n"
+        yaml_file_text        += "${summary_section}"
+        return yaml_file_text
+    }
+
+    //
+    // Exit pipeline if incorrect --genome key provided
+    //
+    private static void genomeExistsError(params, log) {
+        if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
+            log.error "=============================================================================\n" +
+                "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
+                "  Currently, the available genome keys are:\n" +
+                "  ${params.genomes.keySet().join(", ")}\n" +
+                "==================================================================================="
+            System.exit(1)
+        }
+    }
+}
diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar
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diff --git a/main.nf b/main.nf
new file mode 100644
index 00000000..2b33ffe8
--- /dev/null
+++ b/main.nf
@@ -0,0 +1,63 @@
+#!/usr/bin/env nextflow
+/*
+========================================================================================
+    nf-core/rnafusion
+========================================================================================
+    Github : https://github.com/nf-core/rnafusion
+    Website: https://nf-co.re/rnafusion
+    Slack  : https://nfcore.slack.com/channels/rnafusion
+----------------------------------------------------------------------------------------
+*/
+
+nextflow.enable.dsl = 2
+
+/*
+========================================================================================
+    GENOME PARAMETER VALUES
+========================================================================================
+*/
+
+params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
+
+/*
+========================================================================================
+    VALIDATE & PRINT PARAMETER SUMMARY
+========================================================================================
+*/
+
+WorkflowMain.initialise(workflow, params, log)
+
+/*
+========================================================================================
+    NAMED WORKFLOW FOR PIPELINE
+========================================================================================
+*/
+
+include { RNAFUSION } from './workflows/rnafusion'
+
+//
+// WORKFLOW: Run main nf-core/rnafusion analysis pipeline
+//
+workflow NFCORE_RNAFUSION {
+    RNAFUSION ()
+}
+
+/*
+========================================================================================
+    RUN ALL WORKFLOWS
+========================================================================================
+*/
+
+//
+// WORKFLOW: Execute a single named workflow for the pipeline
+// See: https://github.com/nf-core/rnaseq/issues/619
+//
+workflow {
+    NFCORE_RNAFUSION ()
+}
+
+/*
+========================================================================================
+    THE END
+========================================================================================
+*/
diff --git a/modules.json b/modules.json
new file mode 100644
index 00000000..5e4e806f
--- /dev/null
+++ b/modules.json
@@ -0,0 +1,14 @@
+{
+    "name": "nf-core/rnafusion",
+    "homePage": "https://github.com/nf-core/rnafusion",
+    "repos": {
+        "nf-core/modules": {
+            "fastqc": {
+                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+            },
+            "multiqc": {
+                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+            }
+        }
+    }
+}
diff --git a/modules/local/functions.nf b/modules/local/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/local/get_software_versions.nf b/modules/local/get_software_versions.nf
new file mode 100644
index 00000000..2568f170
--- /dev/null
+++ b/modules/local/get_software_versions.nf
@@ -0,0 +1,33 @@
+// Import generic module functions
+include { saveFiles } from './functions'
+
+params.options = [:]
+
+process GET_SOFTWARE_VERSIONS {
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/python:3.8.3"
+    } else {
+        container "quay.io/biocontainers/python:3.8.3"
+    }
+
+    cache false
+
+    input:
+    path versions
+
+    output:
+    path "software_versions.tsv"     , emit: tsv
+    path 'software_versions_mqc.yaml', emit: yaml
+
+    script: // This script is bundled with the pipeline, in nf-core/rnafusion/bin/
+    """
+    echo $workflow.manifest.version > pipeline.version.txt
+    echo $workflow.nextflow.version > nextflow.version.txt
+    scrape_software_versions.py &> software_versions_mqc.yaml
+    """
+}
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
new file mode 100644
index 00000000..5cff46fe
--- /dev/null
+++ b/modules/local/samplesheet_check.nf
@@ -0,0 +1,31 @@
+// Import generic module functions
+include { saveFiles } from './functions'
+
+params.options = [:]
+
+process SAMPLESHEET_CHECK {
+    tag "$samplesheet"
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/python:3.8.3"
+    } else {
+        container "quay.io/biocontainers/python:3.8.3"
+    }
+
+    input:
+    path samplesheet
+
+    output:
+    path '*.csv'
+
+    script: // This script is bundled with the pipeline, in nf-core/rnafusion/bin/
+    """
+    check_samplesheet.py \\
+        $samplesheet \\
+        samplesheet.valid.csv
+    """
+}
diff --git a/modules/nf-core/modules/fastqc/functions.nf b/modules/nf-core/modules/fastqc/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/fastqc/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
new file mode 100644
index 00000000..39c327b2
--- /dev/null
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -0,0 +1,47 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process FASTQC {
+    tag "$meta.id"
+    label 'process_medium'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+
+    conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0"
+    } else {
+        container "quay.io/biocontainers/fastqc:0.11.9--0"
+    }
+
+    input:
+    tuple val(meta), path(reads)
+
+    output:
+    tuple val(meta), path("*.html"), emit: html
+    tuple val(meta), path("*.zip") , emit: zip
+    path  "*.version.txt"          , emit: version
+
+    script:
+    // Add soft-links to original FastQs for consistent naming in pipeline
+    def software = getSoftwareName(task.process)
+    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    if (meta.single_end) {
+        """
+        [ ! -f  ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
+        fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz
+        fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
+        """
+    } else {
+        """
+        [ ! -f  ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
+        [ ! -f  ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
+        fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
+        fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
+        """
+    }
+}
diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml
new file mode 100644
index 00000000..8eb9953d
--- /dev/null
+++ b/modules/nf-core/modules/fastqc/meta.yml
@@ -0,0 +1,51 @@
+name: fastqc
+description: Run FastQC on sequenced reads
+keywords:
+    - quality control
+    - qc
+    - adapters
+    - fastq
+tools:
+    - fastqc:
+        description: |
+            FastQC gives general quality metrics about your reads.
+            It provides information about the quality score distribution
+            across your reads, the per base sequence content (%A/C/G/T).
+            You get information about adapter contamination and other
+            overrepresented sequences.
+        homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
+        documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
+input:
+    - meta:
+        type: map
+        description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+            List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+            respectively.
+output:
+    - meta:
+        type: map
+        description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+    - html:
+        type: file
+        description: FastQC report
+        pattern: "*_{fastqc.html}"
+    - zip:
+        type: file
+        description: FastQC report archive
+        pattern: "*_{fastqc.zip}"
+    - version:
+        type: file
+        description: File containing software version
+        pattern: "*.{version.txt}"
+authors:
+    - "@drpatelh"
+    - "@grst"
+    - "@ewels"
+    - "@FelixKrueger"
diff --git a/modules/nf-core/modules/multiqc/functions.nf b/modules/nf-core/modules/multiqc/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/multiqc/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
new file mode 100644
index 00000000..da780800
--- /dev/null
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -0,0 +1,35 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process MULTIQC {
+    label 'process_medium'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "bioconda::multiqc=1.10.1" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/multiqc:1.10.1--py_0"
+    } else {
+        container "quay.io/biocontainers/multiqc:1.10.1--py_0"
+    }
+
+    input:
+    path multiqc_files
+
+    output:
+    path "*multiqc_report.html", emit: report
+    path "*_data"              , emit: data
+    path "*_plots"             , optional:true, emit: plots
+    path "*.version.txt"       , emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+    """
+    multiqc -f $options.args .
+    multiqc --version | sed -e "s/multiqc, version //g" > ${software}.version.txt
+    """
+}
diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml
new file mode 100644
index 00000000..532a8bb1
--- /dev/null
+++ b/modules/nf-core/modules/multiqc/meta.yml
@@ -0,0 +1,39 @@
+name: MultiQC
+description: Aggregate results from bioinformatics analyses across many samples into a single report
+keywords:
+    - QC
+    - bioinformatics tools
+    - Beautiful stand-alone HTML report
+tools:
+    - multiqc:
+        description: |
+            MultiQC searches a given directory for analysis logs and compiles a HTML report.
+            It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
+        homepage: https://multiqc.info/
+        documentation: https://multiqc.info/docs/
+input:
+    - multiqc_files:
+        type: file
+        description: |
+            List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+output:
+    - report:
+        type: file
+        description: MultiQC report file
+        pattern: "multiqc_report.html"
+    - data:
+        type: dir
+        description: MultiQC data dir
+        pattern: "multiqc_data"
+    - plots:
+        type: file
+        description: Plots created by MultiQC
+        pattern: "*_data"
+    - version:
+        type: file
+        description: File containing software version
+        pattern: "*.{version.txt}"
+authors:
+    - "@abhi18av"
+    - "@bunop"
+    - "@drpatelh"
diff --git a/nextflow.config b/nextflow.config
new file mode 100644
index 00000000..06987dae
--- /dev/null
+++ b/nextflow.config
@@ -0,0 +1,198 @@
+/*
+========================================================================================
+    nf-core/rnafusion Nextflow config file
+========================================================================================
+    Default config options for all compute environments
+----------------------------------------------------------------------------------------
+*/
+
+// Global default params, used in configs
+params {
+
+    // TODO nf-core: Specify your pipeline's command line flags
+    // Input options
+    input                      = null
+
+    // References
+    genome                     = null
+    igenomes_base              = 's3://ngi-igenomes/igenomes'
+    igenomes_ignore            = false
+
+    // MultiQC options
+    multiqc_config             = null
+    multiqc_title              = null
+    max_multiqc_email_size     = '25.MB'
+
+    // Boilerplate options
+    outdir                     = './results'
+    tracedir                   = "${params.outdir}/pipeline_info"
+    publish_dir_mode           = 'copy'
+    email                      = null
+    email_on_fail              = null
+    plaintext_email            = false
+    monochrome_logs            = false
+    help                       = false
+    validate_params            = true
+    show_hidden_params         = false
+    schema_ignore_params       = 'genomes,modules'
+    enable_conda               = false
+    singularity_pull_docker_container = false
+
+    // Config options
+    custom_config_version      = 'master'
+    custom_config_base         = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
+    hostnames                  = [:]
+    config_profile_description = null
+    config_profile_contact     = null
+    config_profile_url         = null
+    config_profile_name        = null
+
+    // Max resource options
+    // Defaults only, expecting to be overwritten
+    max_memory                 = '128.GB'
+    max_cpus                   = 16
+    max_time                   = '240.h'
+
+}
+
+// Load base.config by default for all pipelines
+includeConfig 'conf/base.config'
+
+// Load modules.config for DSL2 module specific options
+includeConfig 'conf/modules.config'
+
+// Load nf-core custom profiles from different Institutions
+try {
+    includeConfig "${params.custom_config_base}/nfcore_custom.config"
+} catch (Exception e) {
+    System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
+}
+
+// Load igenomes.config if required
+if (!params.igenomes_ignore) {
+    includeConfig 'conf/igenomes.config'
+} else {
+    params.genomes = [:]
+}
+
+profiles {
+    debug { process.beforeScript = 'echo $HOSTNAME' }
+    conda {
+        params.enable_conda    = true
+        docker.enabled         = false
+        singularity.enabled    = false
+        podman.enabled         = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
+    }
+    docker {
+        docker.enabled         = true
+        docker.userEmulation   = true
+        singularity.enabled    = false
+        podman.enabled         = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
+    }
+    singularity {
+        singularity.enabled    = true
+        singularity.autoMounts = true
+        docker.enabled         = false
+        podman.enabled         = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
+    }
+    podman {
+        podman.enabled         = true
+        docker.enabled         = false
+        singularity.enabled    = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
+    }
+    shifter {
+        shifter.enabled        = true
+        docker.enabled         = false
+        singularity.enabled    = false
+        podman.enabled         = false
+        charliecloud.enabled   = false
+    }
+    charliecloud {
+        charliecloud.enabled   = true
+        docker.enabled         = false
+        singularity.enabled    = false
+        podman.enabled         = false
+        shifter.enabled        = false
+    }
+    test      { includeConfig 'conf/test.config'      }
+    test_full { includeConfig 'conf/test_full.config' }
+}
+
+// Export these variables to prevent local Python/R libraries from conflicting with those in the container
+env {
+    PYTHONNOUSERSITE = 1
+    R_PROFILE_USER   = "/.Rprofile"
+    R_ENVIRON_USER   = "/.Renviron"
+}
+
+// Capture exit codes from upstream processes when piping
+process.shell = ['/bin/bash', '-euo', 'pipefail']
+
+def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
+timeline {
+    enabled = true
+    file    = "${params.tracedir}/execution_timeline_${trace_timestamp}.html"
+}
+report {
+    enabled = true
+    file    = "${params.tracedir}/execution_report_${trace_timestamp}.html"
+}
+trace {
+    enabled = true
+    file    = "${params.tracedir}/execution_trace_${trace_timestamp}.txt"
+}
+dag {
+    enabled = true
+    file    = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg"
+}
+
+manifest {
+    name            = 'nf-core/rnafusion'
+    author          = '@praveenraj2018'
+    homePage        = 'https://github.com/nf-core/rnafusion'
+    description     = 'RNA sequencing analysis pipeline with curated list of tools for detecting and visualizing fusion genes.'
+    mainScript      = 'main.nf'
+    nextflowVersion = '!>=21.04.0'
+    version         = '1.0dev'
+}
+
+// Function to ensure that resource requirements don't go beyond
+// a maximum limit
+def check_max(obj, type) {
+    if (type == 'memory') {
+        try {
+            if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1)
+                return params.max_memory as nextflow.util.MemoryUnit
+            else
+                return obj
+        } catch (all) {
+            println "   ### ERROR ###   Max memory '${params.max_memory}' is not valid! Using default value: $obj"
+            return obj
+        }
+    } else if (type == 'time') {
+        try {
+            if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1)
+                return params.max_time as nextflow.util.Duration
+            else
+                return obj
+        } catch (all) {
+            println "   ### ERROR ###   Max time '${params.max_time}' is not valid! Using default value: $obj"
+            return obj
+        }
+    } else if (type == 'cpus') {
+        try {
+            return Math.min( obj, params.max_cpus as int )
+        } catch (all) {
+            println "   ### ERROR ###   Max cpus '${params.max_cpus}' is not valid! Using default value: $obj"
+            return obj
+        }
+    }
+}
diff --git a/nextflow_schema.json b/nextflow_schema.json
new file mode 100644
index 00000000..2f692042
--- /dev/null
+++ b/nextflow_schema.json
@@ -0,0 +1,291 @@
+{
+    "$schema": "http://json-schema.org/draft-07/schema",
+    "$id": "https://raw.githubusercontent.com/nf-core/rnafusion/master/nextflow_schema.json",
+    "title": "nf-core/rnafusion pipeline parameters",
+    "description": "RNA sequencing analysis pipeline with curated list of tools for detecting and visualizing fusion genes.",
+    "type": "object",
+    "definitions": {
+        "input_output_options": {
+            "title": "Input/output options",
+            "type": "object",
+            "fa_icon": "fas fa-terminal",
+            "description": "Define where the pipeline should find input data and save output data.",
+            "required": [
+                "input"
+            ],
+            "properties": {
+                "input": {
+                    "type": "string",
+                    "format": "file-path",
+                    "mimetype": "text/csv",
+                    "pattern": "\\.csv$",
+                    "schema": "assets/schema_input.json",
+                    "description": "Path to comma-separated file containing information about the samples in the experiment.",
+                    "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/rnafusion/usage#samplesheet-input).",
+                    "fa_icon": "fas fa-file-csv"
+                },
+                "outdir": {
+                    "type": "string",
+                    "description": "Path to the output directory where the results will be saved.",
+                    "default": "./results",
+                    "fa_icon": "fas fa-folder-open"
+                },
+                "email": {
+                    "type": "string",
+                    "description": "Email address for completion summary.",
+                    "fa_icon": "fas fa-envelope",
+                    "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.",
+                    "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$"
+                },
+                "multiqc_title": {
+                    "type": "string",
+                    "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.",
+                    "fa_icon": "fas fa-file-signature"
+                }
+            }
+        },
+        "reference_genome_options": {
+            "title": "Reference genome options",
+            "type": "object",
+            "fa_icon": "fas fa-dna",
+            "description": "Reference genome related files and options required for the workflow.",
+            "properties": {
+                "genome": {
+                    "type": "string",
+                    "description": "Name of iGenomes reference.",
+                    "fa_icon": "fas fa-book",
+                    "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details."
+                },
+                "fasta": {
+                    "type": "string",
+                    "format": "file-path",
+                    "mimetype": "text/plain",
+                    "pattern": "\\.fn?a(sta)?(\\.gz)?$",
+                    "description": "Path to FASTA genome file.",
+                    "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.",
+                    "fa_icon": "far fa-file-code"
+                },
+                "igenomes_base": {
+                    "type": "string",
+                    "format": "directory-path",
+                    "description": "Directory / URL base for iGenomes references.",
+                    "default": "s3://ngi-igenomes/igenomes",
+                    "fa_icon": "fas fa-cloud-download-alt",
+                    "hidden": true
+                },
+                "igenomes_ignore": {
+                    "type": "boolean",
+                    "description": "Do not load the iGenomes reference config.",
+                    "fa_icon": "fas fa-ban",
+                    "hidden": true,
+                    "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
+                }
+            }
+        },
+        "institutional_config_options": {
+            "title": "Institutional config options",
+            "type": "object",
+            "fa_icon": "fas fa-university",
+            "description": "Parameters used to describe centralised config profiles. These should not be edited.",
+            "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.",
+            "properties": {
+                "custom_config_version": {
+                    "type": "string",
+                    "description": "Git commit id for Institutional configs.",
+                    "default": "master",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "custom_config_base": {
+                    "type": "string",
+                    "description": "Base directory for Institutional configs.",
+                    "default": "https://raw.githubusercontent.com/nf-core/configs/master",
+                    "hidden": true,
+                    "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.",
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "hostnames": {
+                    "type": "string",
+                    "description": "Institutional configs hostname.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "config_profile_name": {
+                    "type": "string",
+                    "description": "Institutional config name.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "config_profile_description": {
+                    "type": "string",
+                    "description": "Institutional config description.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "config_profile_contact": {
+                    "type": "string",
+                    "description": "Institutional config contact information.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                },
+                "config_profile_url": {
+                    "type": "string",
+                    "description": "Institutional config URL link.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-users-cog"
+                }
+            }
+        },
+        "max_job_request_options": {
+            "title": "Max job request options",
+            "type": "object",
+            "fa_icon": "fab fa-acquisitions-incorporated",
+            "description": "Set the top limit for requested resources for any single job.",
+            "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.",
+            "properties": {
+                "max_cpus": {
+                    "type": "integer",
+                    "description": "Maximum number of CPUs that can be requested for any single job.",
+                    "default": 16,
+                    "fa_icon": "fas fa-microchip",
+                    "hidden": true,
+                    "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`"
+                },
+                "max_memory": {
+                    "type": "string",
+                    "description": "Maximum amount of memory that can be requested for any single job.",
+                    "default": "128.GB",
+                    "fa_icon": "fas fa-memory",
+                    "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$",
+                    "hidden": true,
+                    "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`"
+                },
+                "max_time": {
+                    "type": "string",
+                    "description": "Maximum amount of time that can be requested for any single job.",
+                    "default": "240.h",
+                    "fa_icon": "far fa-clock",
+                    "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$",
+                    "hidden": true,
+                    "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`"
+                }
+            }
+        },
+        "generic_options": {
+            "title": "Generic options",
+            "type": "object",
+            "fa_icon": "fas fa-file-import",
+            "description": "Less common options for the pipeline, typically set in a config file.",
+            "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
+            "properties": {
+                "help": {
+                    "type": "boolean",
+                    "description": "Display help text.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "publish_dir_mode": {
+                    "type": "string",
+                    "default": "copy",
+                    "description": "Method used to save pipeline results to output directory.",
+                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
+                    "fa_icon": "fas fa-copy",
+                    "enum": [
+                        "symlink",
+                        "rellink",
+                        "link",
+                        "copy",
+                        "copyNoFollow",
+                        "move"
+                    ],
+                    "hidden": true
+                },
+                "email_on_fail": {
+                    "type": "string",
+                    "description": "Email address for completion summary, only when pipeline fails.",
+                    "fa_icon": "fas fa-exclamation-triangle",
+                    "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$",
+                    "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.",
+                    "hidden": true
+                },
+                "plaintext_email": {
+                    "type": "boolean",
+                    "description": "Send plain-text email instead of HTML.",
+                    "fa_icon": "fas fa-remove-format",
+                    "hidden": true
+                },
+                "max_multiqc_email_size": {
+                    "type": "string",
+                    "description": "File size limit when attaching MultiQC reports to summary emails.",
+                    "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$",
+                    "default": "25.MB",
+                    "fa_icon": "fas fa-file-upload",
+                    "hidden": true
+                },
+                "monochrome_logs": {
+                    "type": "boolean",
+                    "description": "Do not use coloured log outputs.",
+                    "fa_icon": "fas fa-palette",
+                    "hidden": true
+                },
+                "multiqc_config": {
+                    "type": "string",
+                    "description": "Custom config file to supply to MultiQC.",
+                    "fa_icon": "fas fa-cog",
+                    "hidden": true
+                },
+                "tracedir": {
+                    "type": "string",
+                    "description": "Directory to keep pipeline Nextflow logs and reports.",
+                    "default": "${params.outdir}/pipeline_info",
+                    "fa_icon": "fas fa-cogs",
+                    "hidden": true
+                },
+                "validate_params": {
+                    "type": "boolean",
+                    "description": "Boolean whether to validate parameters against the schema at runtime",
+                    "default": true,
+                    "fa_icon": "fas fa-check-square",
+                    "hidden": true
+                },
+                "show_hidden_params": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-eye-slash",
+                    "description": "Show all params when using `--help`",
+                    "hidden": true,
+                    "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters."
+                },
+                "enable_conda": {
+                    "type": "boolean",
+                    "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-bacon"
+                },
+                "singularity_pull_docker_container": {
+                    "type": "boolean",
+                    "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues."
+                }
+            }
+        }
+    },
+    "allOf": [
+        {
+            "$ref": "#/definitions/input_output_options"
+        },
+        {
+            "$ref": "#/definitions/reference_genome_options"
+        },
+        {
+            "$ref": "#/definitions/institutional_config_options"
+        },
+        {
+            "$ref": "#/definitions/max_job_request_options"
+        },
+        {
+            "$ref": "#/definitions/generic_options"
+        }
+    ]
+}
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
new file mode 100644
index 00000000..b664bc8c
--- /dev/null
+++ b/subworkflows/local/input_check.nf
@@ -0,0 +1,42 @@
+//
+// Check input samplesheet and get read channels
+//
+
+params.options = [:]
+
+include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options )
+
+workflow INPUT_CHECK {
+    take:
+    samplesheet // file: /path/to/samplesheet.csv
+
+    main:
+    SAMPLESHEET_CHECK ( samplesheet )
+        .splitCsv ( header:true, sep:',' )
+        .map { create_fastq_channels(it) }
+        .set { reads }
+
+    emit:
+    reads // channel: [ val(meta), [ reads ] ]
+}
+
+// Function to get list of [ meta, [ fastq_1, fastq_2 ] ]
+def create_fastq_channels(LinkedHashMap row) {
+    def meta = [:]
+    meta.id           = row.sample
+    meta.single_end   = row.single_end.toBoolean()
+
+    def array = []
+    if (!file(row.fastq_1).exists()) {
+        exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}"
+    }
+    if (meta.single_end) {
+        array = [ meta, [ file(row.fastq_1) ] ]
+    } else {
+        if (!file(row.fastq_2).exists()) {
+            exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}"
+        }
+        array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ]
+    }
+    return array
+}
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
new file mode 100644
index 00000000..0728b486
--- /dev/null
+++ b/workflows/rnafusion.nf
@@ -0,0 +1,141 @@
+/*
+========================================================================================
+    VALIDATE INPUTS
+========================================================================================
+*/
+
+def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
+
+// Validate input parameters
+WorkflowRnafusion.initialise(params, log)
+
+// TODO nf-core: Add all file path parameters for the pipeline to the list below
+// Check input path parameters to see if they exist
+def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ]
+for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
+
+// Check mandatory parameters
+if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' }
+
+/*
+========================================================================================
+    CONFIG FILES
+========================================================================================
+*/
+
+ch_multiqc_config        = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true)
+ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
+
+/*
+========================================================================================
+    IMPORT LOCAL MODULES/SUBWORKFLOWS
+========================================================================================
+*/
+
+// Don't overwrite global params.modules, create a copy instead and use that within the main script.
+def modules = params.modules.clone()
+
+//
+// MODULE: Local to the pipeline
+//
+include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] )
+
+//
+// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
+//
+include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] )
+
+/*
+========================================================================================
+    IMPORT NF-CORE MODULES/SUBWORKFLOWS
+========================================================================================
+*/
+
+def multiqc_options   = modules['multiqc']
+multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : ''
+
+//
+// MODULE: Installed directly from nf-core/modules
+//
+include { FASTQC  } from '../modules/nf-core/modules/fastqc/main'  addParams( options: modules['fastqc'] )
+include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options   )
+
+/*
+========================================================================================
+    RUN MAIN WORKFLOW
+========================================================================================
+*/
+
+// Info required for completion email and summary
+def multiqc_report = []
+
+workflow RNAFUSION {
+
+    ch_software_versions = Channel.empty()
+
+    //
+    // SUBWORKFLOW: Read in samplesheet, validate and stage input files
+    //
+    INPUT_CHECK (
+        ch_input
+    )
+
+    //
+    // MODULE: Run FastQC
+    //
+    FASTQC (
+        INPUT_CHECK.out.reads
+    )
+    ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null))
+
+    //
+    // MODULE: Pipeline reporting
+    //
+    ch_software_versions
+        .map { it -> if (it) [ it.baseName, it ] }
+        .groupTuple()
+        .map { it[1][0] }
+        .flatten()
+        .collect()
+        .set { ch_software_versions }
+
+    GET_SOFTWARE_VERSIONS (
+        ch_software_versions.map { it }.collect()
+    )
+
+    //
+    // MODULE: MultiQC
+    //
+    workflow_summary    = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params)
+    ch_workflow_summary = Channel.value(workflow_summary)
+
+    ch_multiqc_files = Channel.empty()
+    ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect())
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+
+    MULTIQC (
+        ch_multiqc_files.collect()
+    )
+    multiqc_report       = MULTIQC.out.report.toList()
+    ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null))
+}
+
+/*
+========================================================================================
+    COMPLETION EMAIL AND SUMMARY
+========================================================================================
+*/
+
+workflow.onComplete {
+    NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
+    NfcoreTemplate.summary(workflow, params, log)
+}
+
+/*
+========================================================================================
+    THE END
+========================================================================================
+*/

From 4e93a41b1154d14153ffd3200a9dc4a204b048d2 Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Thu, 29 Jul 2021 12:19:16 +0200
Subject: [PATCH 031/359] Base DSL2 template with QC modules

---
 README.md                               | 14 +-------------
 bin/check_samplesheet.py                |  6 +-----
 conf/base.config                        |  8 --------
 conf/test.config                        |  7 +++----
 conf/test_full.config                   |  9 ++++-----
 modules.json                            |  4 ++--
 modules/nf-core/modules/multiqc/main.nf |  6 +++---
 nextflow.config                         |  1 -
 workflows/rnafusion.nf                  |  9 ++++++---
 9 files changed, 20 insertions(+), 44 deletions(-)

diff --git a/README.md b/README.md
index b5340698..46a67684 100644
--- a/README.md
+++ b/README.md
@@ -16,18 +16,14 @@
 
 ## Introduction
 
-<!-- TODO nf-core: Write a 1-2 sentence summary of what data the pipeline is for and what it does -->
 **nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing analysis pipeline with curated list of tools for detecting and visualizing fusion genes..
 
 The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
 
-<!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
 On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).
 
 ## Pipeline summary
 
-<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
-
 1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
 2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 
@@ -49,10 +45,8 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 4. Start running your own analysis!
 
-    <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
-
     ```console
-    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input samplesheet.csv --genome GRCh37
+    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input samplesheet.csv --genome GRCh38
     ```
 
 ## Documentation
@@ -65,8 +59,6 @@ nf-core/rnafusion was originally written by @praveenraj2018.
 
 We thank the following people for their extensive assistance in the development of this pipeline:
 
-<!-- TODO nf-core: If applicable, make list of people who have also contributed -->
-
 ## Contributions and Support
 
 If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).
@@ -75,10 +67,6 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 
 ## Citations
 
-<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->
-<!-- If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
-
-<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
 
 You can cite the `nf-core` publication as follows:
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index dff05443..6874c70c 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -1,8 +1,5 @@
 #!/usr/bin/env python
 
-# TODO nf-core: Update the script to check the samplesheet
-# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
-
 import os
 import sys
 import errno
@@ -38,7 +35,6 @@ def print_error(error, context="Line", context_str=""):
     sys.exit(1)
 
 
-# TODO nf-core: Update the check_samplesheet function
 def check_samplesheet(file_in, file_out):
     """
     This function checks that the samplesheet follows the following structure:
@@ -57,7 +53,7 @@ def check_samplesheet(file_in, file_out):
 
         ## Check header
         MIN_COLS = 2
-        # TODO nf-core: Update the column names for the input samplesheet
+
         HEADER = ["sample", "fastq_1", "fastq_2"]
         header = [x.strip('"') for x in fin.readline().strip().split(",")]
         if header[: len(HEADER)] != HEADER:
diff --git a/conf/base.config b/conf/base.config
index 5619f83a..f4ce0af2 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -10,7 +10,6 @@
 
 process {
 
-    // TODO nf-core: Check the defaults for all processes
     cpus   = { check_max( 1    * task.attempt, 'cpus'   ) }
     memory = { check_max( 6.GB * task.attempt, 'memory' ) }
     time   = { check_max( 4.h  * task.attempt, 'time'   ) }
@@ -19,13 +18,6 @@ process {
     maxRetries    = 1
     maxErrors     = '-1'
 
-    // Process-specific resource requirements
-    // NOTE - Please try and re-use the labels below as much as possible.
-    //        These labels are used and recognised by default in DSL2 files hosted on nf-core/modules.
-    //        If possible, it would be nice to keep the same label naming convention when
-    //        adding in your local modules too.
-    // TODO nf-core: Customise requirements for specific processes.
-    // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors
     withLabel:process_low {
         cpus   = { check_max( 2     * task.attempt, 'cpus'    ) }
         memory = { check_max( 12.GB * task.attempt, 'memory'  ) }
diff --git a/conf/test.config b/conf/test.config
index 331ff129..31a9c170 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -20,10 +20,9 @@ params {
     max_time   = 6.h
 
     // Input data
-    // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
-    // TODO nf-core: Give any required params for the test so that command line flags are not needed
-    input  = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
+    input = 'assets/samplesheet_valid.csv'
 
     // Genome references
-    genome = 'R64-1-1'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
+    fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
 }
diff --git a/conf/test_full.config b/conf/test_full.config
index 260cb93f..59beea62 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -14,11 +14,10 @@ params {
     config_profile_name        = 'Full test profile'
     config_profile_description = 'Full test dataset to check pipeline function'
 
-    // Input data for full size test
-    // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA)
-    // TODO nf-core: Give any required params for the test so that command line flags are not needed
-    input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv'
+    // Input data
+    input = 'assets/samplesheet_valid.csv'
 
     // Genome references
-    genome = 'R64-1-1'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
+    fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
 }
diff --git a/modules.json b/modules.json
index 5e4e806f..5664b305 100644
--- a/modules.json
+++ b/modules.json
@@ -7,8 +7,8 @@
                 "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
             },
             "multiqc": {
-                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+                "git_sha": "c5235a983d454787fa0c3247b02086969217163b"
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index da780800..8b6d6f0c 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -10,11 +10,11 @@ process MULTIQC {
         mode: params.publish_dir_mode,
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
-    conda (params.enable_conda ? "bioconda::multiqc=1.10.1" : null)
+    conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/multiqc:1.10.1--py_0"
+        container "https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0"
     } else {
-        container "quay.io/biocontainers/multiqc:1.10.1--py_0"
+        container "quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0"
     }
 
     input:
diff --git a/nextflow.config b/nextflow.config
index 06987dae..927863d6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -9,7 +9,6 @@
 // Global default params, used in configs
 params {
 
-    // TODO nf-core: Specify your pipeline's command line flags
     // Input options
     input                      = null
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 0728b486..27173f52 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -9,9 +9,12 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
 // Validate input parameters
 WorkflowRnafusion.initialise(params, log)
 
-// TODO nf-core: Add all file path parameters for the pipeline to the list below
-// Check input path parameters to see if they exist
-def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ]
+def checkPathParamList = [
+    params.input,
+    params.multiqc_config,
+    params.fasta
+]
+
 for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
 
 // Check mandatory parameters

From e1fe47867a0c34c37489e3a99e06f43bafd4059d Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Thu, 29 Jul 2021 13:44:46 +0200
Subject: [PATCH 032/359] Added sample sheet having human rnaseq data

---
 .github/PULL_REQUEST_TEMPLATE.md | 2 +-
 .github/workflows/ci.yml         | 3 ---
 2 files changed, 1 insertion(+), 4 deletions(-)

diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 3760ac7b..026428de 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
     - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
     - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
-- [ ] Make sure your code lints (`nf-core lint .`).
+- [ ] Make sure your code lints (`nf-core lint`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 1145652b..c826b074 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -36,8 +36,5 @@ jobs:
           sudo mv nextflow /usr/local/bin/
 
       - name: Run pipeline with test data
-        # TODO nf-core: You can customise CI pipeline run tests as required
-        # For example: adding multiple test runs with different parameters
-        # Remember that you can parallelise this by using strategy.matrix
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker

From 07cc8dce891daf1f4bb5ea2b8663b9bc398f72cc Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Thu, 29 Jul 2021 13:45:20 +0200
Subject: [PATCH 033/359] Added sample sheet having human rnaseq data

---
 assets/samplesheet_valid.csv | 2 ++
 1 file changed, 2 insertions(+)
 create mode 100644 assets/samplesheet_valid.csv

diff --git a/assets/samplesheet_valid.csv b/assets/samplesheet_valid.csv
new file mode 100644
index 00000000..7131bd5d
--- /dev/null
+++ b/assets/samplesheet_valid.csv
@@ -0,0 +1,2 @@
+sample,fastq_1,fastq_2,strandedness
+GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse

From e4c2a9e34ff344ad46128f288a0ffe8021aba5f4 Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Thu, 29 Jul 2021 13:49:27 +0200
Subject: [PATCH 034/359] Fix: lint errors

---
 .github/CONTRIBUTING.md                |  6 +++---
 .github/workflows/linting.yml          |  2 +-
 bin/scrape_software_versions.py        |  2 +-
 lib/NfcoreTemplate.groovy              | 22 +++++++++++++---------
 modules/local/get_software_versions.nf |  1 +
 modules/local/samplesheet_check.nf     |  1 +
 6 files changed, 20 insertions(+), 14 deletions(-)

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 06a1bbd5..1912ed9d 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -19,7 +19,7 @@ If you'd like to write some code for nf-core/rnafusion, the standard workflow is
     * If there isn't one already, please create one so that others know you're working on this
 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rnafusion repository](https://github.com/nf-core/rnafusion) to your GitHub account
 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
-4. Use `nf-core schema build .` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
+4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged
 
 If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/).
@@ -69,7 +69,7 @@ If you wish to contribute a new step, please use the following coding standards:
 2. Write the process block (see below).
 3. Define the output channel if needed (see below).
 4. Add any new flags/options to `nextflow.config` with a default (see below).
-5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`).
+5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build`).
 6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter).
 7. Add sanity checks for all relevant parameters.
 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`.
@@ -83,7 +83,7 @@ If you wish to contribute a new step, please use the following coding standards:
 
 Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope.
 
-Once there, use `nf-core schema build .` to add to `nextflow_schema.json`.
+Once there, use `nf-core schema build` to add to `nextflow_schema.json`.
 
 ### Default processes resource requirements
 
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index fbde5cf8..3b448773 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -53,7 +53,7 @@ jobs:
 
       - uses: actions/setup-node@v1
         with:
-          node-version: "10"
+          node-version: '10'
 
       - name: Install editorconfig-checker
         run: npm install -g editorconfig-checker
diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
index 590af3bb..ee132097 100755
--- a/bin/scrape_software_versions.py
+++ b/bin/scrape_software_versions.py
@@ -30,7 +30,7 @@
     print("        <dt>{}</dt><dd><samp>{}</samp></dd>".format(k, v))
 print("    </dl>")
 
-# Write out regexes as csv file:
+# Write out as tsv file:
 with open("software_versions.tsv", "w") as f:
     for k, v in sorted(results.items()):
         f.write("{}\t{}\n".format(k, v))
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index b6e689ec..44551e0a 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -24,17 +24,21 @@ class NfcoreTemplate {
     public static void hostName(workflow, params, log) {
         Map colors = logColours(params.monochrome_logs)
         if (params.hostnames) {
-            def hostname = "hostname".execute().text.trim()
-            params.hostnames.each { prof, hnames ->
-                hnames.each { hname ->
-                    if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                        log.info "=${colors.yellow}====================================================${colors.reset}=\n" +
-                            "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" +
-                            "      but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" +
-                            "      ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" +
-                            "=${colors.yellow}====================================================${colors.reset}="
+            try {
+                def hostname = "hostname".execute().text.trim()
+                params.hostnames.each { prof, hnames ->
+                    hnames.each { hname ->
+                        if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
+                            log.info "=${colors.yellow}====================================================${colors.reset}=\n" +
+                                "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" +
+                                "      but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" +
+                                "      ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" +
+                                "=${colors.yellow}====================================================${colors.reset}="
+                        }
                     }
                 }
+            } catch (Exception e) {
+                log.warn "[$workflow.manifest.name] Could not determine 'hostname' - skipping check. Reason: ${e.message}."
             }
         }
     }
diff --git a/modules/local/get_software_versions.nf b/modules/local/get_software_versions.nf
index 2568f170..d787cbfc 100644
--- a/modules/local/get_software_versions.nf
+++ b/modules/local/get_software_versions.nf
@@ -4,6 +4,7 @@ include { saveFiles } from './functions'
 params.options = [:]
 
 process GET_SOFTWARE_VERSIONS {
+    label 'process_low'
     publishDir "${params.outdir}",
         mode: params.publish_dir_mode,
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) }
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index 5cff46fe..15586d90 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -4,6 +4,7 @@ include { saveFiles } from './functions'
 params.options = [:]
 
 process SAMPLESHEET_CHECK {
+    label 'process_low'
     tag "$samplesheet"
     publishDir "${params.outdir}",
         mode: params.publish_dir_mode,

From a347df9efa923d79c184cd0f27f42c41802ddfd0 Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Thu, 29 Jul 2021 16:59:33 +0200
Subject: [PATCH 035/359] Added genome prep, star align and arriba fusion
 calling steps

---
 conf/modules.config                           | 14 ++++
 conf/test.config                              |  4 +
 conf/test_full.config                         |  4 +
 modules.json                                  | 18 +++++
 modules/nf-core/modules/arriba/functions.nf   | 68 ++++++++++++++++
 modules/nf-core/modules/arriba/main.nf        | 47 +++++++++++
 modules/nf-core/modules/arriba/meta.yml       | 54 +++++++++++++
 modules/nf-core/modules/gunzip/functions.nf   | 68 ++++++++++++++++
 modules/nf-core/modules/gunzip/main.nf        | 35 ++++++++
 modules/nf-core/modules/gunzip/meta.yml       | 27 +++++++
 .../modules/samtools/faidx/functions.nf       | 68 ++++++++++++++++
 .../nf-core/modules/samtools/faidx/main.nf    | 34 ++++++++
 .../nf-core/modules/samtools/faidx/meta.yml   | 32 ++++++++
 .../nf-core/modules/star/align/functions.nf   | 68 ++++++++++++++++
 modules/nf-core/modules/star/align/main.nf    | 72 +++++++++++++++++
 modules/nf-core/modules/star/align/meta.yml   | 80 +++++++++++++++++++
 .../modules/star/genomegenerate/functions.nf  | 68 ++++++++++++++++
 .../modules/star/genomegenerate/main.nf       | 67 ++++++++++++++++
 .../modules/star/genomegenerate/meta.yml      | 36 +++++++++
 modules/nf-core/modules/untar/functions.nf    | 68 ++++++++++++++++
 modules/nf-core/modules/untar/main.nf         | 35 ++++++++
 modules/nf-core/modules/untar/meta.yml        | 27 +++++++
 nextflow.config                               |  7 ++
 subworkflows/local/prepare_genome.nf          | 63 +++++++++++++++
 workflows/rnafusion.nf                        | 59 +++++++++++---
 25 files changed, 1112 insertions(+), 11 deletions(-)
 create mode 100644 modules/nf-core/modules/arriba/functions.nf
 create mode 100644 modules/nf-core/modules/arriba/main.nf
 create mode 100644 modules/nf-core/modules/arriba/meta.yml
 create mode 100644 modules/nf-core/modules/gunzip/functions.nf
 create mode 100644 modules/nf-core/modules/gunzip/main.nf
 create mode 100644 modules/nf-core/modules/gunzip/meta.yml
 create mode 100644 modules/nf-core/modules/samtools/faidx/functions.nf
 create mode 100644 modules/nf-core/modules/samtools/faidx/main.nf
 create mode 100644 modules/nf-core/modules/samtools/faidx/meta.yml
 create mode 100644 modules/nf-core/modules/star/align/functions.nf
 create mode 100644 modules/nf-core/modules/star/align/main.nf
 create mode 100644 modules/nf-core/modules/star/align/meta.yml
 create mode 100644 modules/nf-core/modules/star/genomegenerate/functions.nf
 create mode 100644 modules/nf-core/modules/star/genomegenerate/main.nf
 create mode 100644 modules/nf-core/modules/star/genomegenerate/meta.yml
 create mode 100644 modules/nf-core/modules/untar/functions.nf
 create mode 100644 modules/nf-core/modules/untar/main.nf
 create mode 100644 modules/nf-core/modules/untar/meta.yml
 create mode 100644 subworkflows/local/prepare_genome.nf

diff --git a/conf/modules.config b/conf/modules.config
index 0b1bfdec..0c4073de 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -28,5 +28,19 @@ params {
         'multiqc' {
             args = ""
         }
+       'star_genomegenerate' {
+            args             = ""
+            publish_dir      = 'genome/index'
+        }
+        'star_align' {
+            args            = "--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
+            publish_dir     = "${params.aligner}"
+            publish_files   = ['bam':'', 'junction':'', 'out':'log', 'tab':'log']
+        }
+        'arriba_fusion' {
+            args            = ""
+            publish_dir     = 'arriba'
+            publish_files   = ['fusions':'', 'fusions_fail':'']
+        }
     }
 }
diff --git a/conf/test.config b/conf/test.config
index 31a9c170..f5ed8827 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -25,4 +25,8 @@ params {
     // Genome references
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
     fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
+    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf'
+
+    //STAR index
+    star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
 }
diff --git a/conf/test_full.config b/conf/test_full.config
index 59beea62..31e5e9c2 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -20,4 +20,8 @@ params {
     // Genome references
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
     fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
+    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf'
+
+    //STAR index
+    star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
 }
diff --git a/modules.json b/modules.json
index 5664b305..c20f9732 100644
--- a/modules.json
+++ b/modules.json
@@ -3,11 +3,29 @@
     "homePage": "https://github.com/nf-core/rnafusion",
     "repos": {
         "nf-core/modules": {
+            "arriba": {
+                "git_sha": "3cabc95d0ed8a5a4e07b8f9b1d1f7ff9a70f61e1"
+            },
             "fastqc": {
                 "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
             },
+            "gunzip": {
+                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+            },
             "multiqc": {
                 "git_sha": "c5235a983d454787fa0c3247b02086969217163b"
+            },
+            "samtools/faidx": {
+                "git_sha": "c5235a983d454787fa0c3247b02086969217163b"
+            },
+            "star/align": {
+                "git_sha": "df909015b6651dadb52626cf471f15a6d33985a2"
+            },
+            "star/genomegenerate": {
+                "git_sha": "f43778b0e641a9643ae73fe1f2fe2d5bb81edffc"
+            },
+            "untar": {
+                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
             }
         }
     }
diff --git a/modules/nf-core/modules/arriba/functions.nf b/modules/nf-core/modules/arriba/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/arriba/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/arriba/main.nf b/modules/nf-core/modules/arriba/main.nf
new file mode 100644
index 00000000..739922ef
--- /dev/null
+++ b/modules/nf-core/modules/arriba/main.nf
@@ -0,0 +1,47 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process ARRIBA {
+    tag "$meta.id"
+    label 'process_medium'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+
+    conda (params.enable_conda ? "bioconda::arriba=2.1.0" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/arriba:2.1.0--h3198e80_1"
+    } else {
+        container "quay.io/biocontainers/arriba:2.1.0--h3198e80_1"
+    }
+
+    input:
+    tuple val(meta), path(bam)
+    path fasta
+    path gtf
+
+    output:
+    tuple val(meta), path("*.fusions.tsv")          , emit: fusions
+    tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail
+    path "*.version.txt"                            , emit: version
+
+    script:
+    def software  = getSoftwareName(task.process)
+    def prefix    = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def blacklist = (options.args.contains('-b')) ? '' : '-f blacklist'
+    """
+    arriba \\
+        -x $bam \\
+        -a $fasta \\
+        -g $gtf \\
+        -o ${prefix}.fusions.tsv \\
+        -O ${prefix}.fusions.discarded.tsv \\
+        $blacklist \\
+        $options.args
+
+    echo \$(arriba -h | grep 'Version:' 2>&1) |  sed 's/Version:\s//' > ${software}.version.txt
+    """
+}
diff --git a/modules/nf-core/modules/arriba/meta.yml b/modules/nf-core/modules/arriba/meta.yml
new file mode 100644
index 00000000..370f82ec
--- /dev/null
+++ b/modules/nf-core/modules/arriba/meta.yml
@@ -0,0 +1,54 @@
+name: arriba
+description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
+keywords:
+  - fusion
+  - arriba
+tools:
+  - arriba:
+      description: Fast and accurate gene fusion detection from RNA-Seq data
+      homepage: https://github.com/suhrig/arriba
+      documentation: https://arriba.readthedocs.io/en/latest/
+      tool_dev_url: https://github.com/suhrig/arriba
+      doi: "10.1101/gr.257246.119"
+      licence: ['MIT']
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - fasta:
+      type: file
+      description: Assembly FASTA file
+      pattern: "*.{fasta}"
+  - gtf:
+      type: file
+      description: Annotation GTF file
+      pattern: "*.{gtf}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+  - fusions:
+      type: file
+      description: File contains fusions which pass all of Arriba's filters.
+      pattern: "*.{fusions.tsv}"
+  - fusions_fail:
+      type: file
+      description: File contains fusions that Arriba classified as an artifact or that are also observed in healthy tissue.
+      pattern: "*.{fusions.discarded.tsv}"
+
+authors:
+  - "@praveenraj2018"
diff --git a/modules/nf-core/modules/gunzip/functions.nf b/modules/nf-core/modules/gunzip/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/gunzip/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/gunzip/main.nf b/modules/nf-core/modules/gunzip/main.nf
new file mode 100644
index 00000000..29248796
--- /dev/null
+++ b/modules/nf-core/modules/gunzip/main.nf
@@ -0,0 +1,35 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process GUNZIP {
+    tag "$archive"
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img"
+    } else {
+        container "biocontainers/biocontainers:v1.2.0_cv1"
+    }
+
+    input:
+    path archive
+
+    output:
+    path "$gunzip",       emit: gunzip
+    path "*.version.txt", emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+    gunzip       = archive.toString() - '.gz'
+    """
+    gunzip -f $options.args $archive
+    echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//' > ${software}.version.txt
+    """
+}
diff --git a/modules/nf-core/modules/gunzip/meta.yml b/modules/nf-core/modules/gunzip/meta.yml
new file mode 100644
index 00000000..922e74e6
--- /dev/null
+++ b/modules/nf-core/modules/gunzip/meta.yml
@@ -0,0 +1,27 @@
+name: gunzip
+description: Compresses and decompresses files.
+keywords:
+    - gunzip
+    - compression
+tools:
+    - gunzip:
+        description: |
+            gzip is a file format and a software application used for file compression and decompression.
+        documentation: https://www.gnu.org/software/gzip/manual/gzip.html
+input:
+    - archive:
+        type: file
+        description: File to be compressed/uncompressed
+        pattern: "*.*"
+output:
+    - gunzip:
+        type: file
+        description: Compressed/uncompressed file
+        pattern: "*.*"
+    - version:
+        type: file
+        description: File containing software version
+        pattern: "*.{version.txt}"
+authors:
+    - "@joseespinosa"
+    - "@drpatelh"
diff --git a/modules/nf-core/modules/samtools/faidx/functions.nf b/modules/nf-core/modules/samtools/faidx/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/samtools/faidx/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf
new file mode 100644
index 00000000..a89ff2bb
--- /dev/null
+++ b/modules/nf-core/modules/samtools/faidx/main.nf
@@ -0,0 +1,34 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process SAMTOOLS_FAIDX {
+    tag "$fasta"
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? 'bioconda::samtools=1.13' : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0"
+    } else {
+        container "quay.io/biocontainers/samtools:1.13--h8c37831_0"
+    }
+
+    input:
+    path fasta
+
+    output:
+    path "*.fai"        , emit: fai
+    path "*.version.txt", emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+    """
+    samtools faidx $fasta
+    echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt
+    """
+}
diff --git a/modules/nf-core/modules/samtools/faidx/meta.yml b/modules/nf-core/modules/samtools/faidx/meta.yml
new file mode 100644
index 00000000..f92234d0
--- /dev/null
+++ b/modules/nf-core/modules/samtools/faidx/meta.yml
@@ -0,0 +1,32 @@
+name: samtools_faidx
+description: Index FASTA file
+keywords:
+    - index
+    - fasta
+tools:
+    - samtools:
+        description: |
+            SAMtools is a set of utilities for interacting with and post-processing
+            short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+            These files are generated as output by short read aligners like BWA.
+        homepage: http://www.htslib.org/
+        documentation: http://www.htslib.org/doc/samtools.html
+        doi: 10.1093/bioinformatics/btp352
+input:
+    - fasta:
+        type: file
+        description: FASTA file
+        pattern: "*.{fa,fasta}"
+output:
+    - fai:
+        type: file
+        description: FASTA index file
+        pattern: "*.{fai}"
+    - version:
+        type: file
+        description: File containing software version
+        pattern: "*.{version.txt}"
+authors:
+    - "@drpatelh"
+    - "@ewels"
+    - "@phue"
diff --git a/modules/nf-core/modules/star/align/functions.nf b/modules/nf-core/modules/star/align/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/star/align/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf
new file mode 100644
index 00000000..c06daf24
--- /dev/null
+++ b/modules/nf-core/modules/star/align/main.nf
@@ -0,0 +1,72 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process STAR_ALIGN {
+    tag "$meta.id"
+    label 'process_high'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+
+    // Note: 2.7X indices incompatible with AWS iGenomes.
+    conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container 'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0'
+    } else {
+        container 'quay.io/biocontainers/star:2.7.9a--h9ee0642_0'
+    }
+
+    input:
+    tuple val(meta), path(reads)
+    path  index
+    path  gtf
+
+    output:
+    tuple val(meta), path('*d.out.bam')       , emit: bam
+    tuple val(meta), path('*Log.final.out')   , emit: log_final
+    tuple val(meta), path('*Log.out')         , emit: log_out
+    tuple val(meta), path('*Log.progress.out'), emit: log_progress
+    path  '*.version.txt'                     , emit: version
+
+    tuple val(meta), path('*sortedByCoord.out.bam')  , optional:true, emit: bam_sorted
+    tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript
+    tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted
+    tuple val(meta), path('*fastq.gz')               , optional:true, emit: fastq
+    tuple val(meta), path('*.tab')                   , optional:true, emit: tab
+    tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
+
+    script:
+    def software   = getSoftwareName(task.process)
+    def prefix     = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
+    def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix'"
+    def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
+    def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
+    """
+    STAR \\
+        --genomeDir $index \\
+        --readFilesIn $reads  \\
+        --runThreadN $task.cpus \\
+        --outFileNamePrefix $prefix. \\
+        $out_sam_type \\
+        $ignore_gtf \\
+        $seq_center \\
+        $options.args
+
+    $mv_unsorted_bam
+
+    if [ -f ${prefix}.Unmapped.out.mate1 ]; then
+        mv ${prefix}.Unmapped.out.mate1 ${prefix}.unmapped_1.fastq
+        gzip ${prefix}.unmapped_1.fastq
+    fi
+    if [ -f ${prefix}.Unmapped.out.mate2 ]; then
+        mv ${prefix}.Unmapped.out.mate2 ${prefix}.unmapped_2.fastq
+        gzip ${prefix}.unmapped_2.fastq
+    fi
+
+    STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt
+    """
+}
diff --git a/modules/nf-core/modules/star/align/meta.yml b/modules/nf-core/modules/star/align/meta.yml
new file mode 100644
index 00000000..a589d145
--- /dev/null
+++ b/modules/nf-core/modules/star/align/meta.yml
@@ -0,0 +1,80 @@
+name: star_align
+description: Align reads to a reference genome using STAR
+keywords:
+  - align
+  - fasta
+  - genome
+  - reference
+tools:
+  - star:
+      description: |
+        STAR is a software package for mapping DNA sequences against
+        a large reference genome, such as the human genome.
+      homepage: https://github.com/alexdobin/STAR
+      manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
+      doi: 10.1093/bioinformatics/bts635
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
+  - index:
+      type: directory
+      description: STAR genome index
+      pattern: "star"
+output:
+  - bam:
+      type: file
+      description: Output BAM file containing read alignments
+      pattern: "*.{bam}"
+  - log_final:
+      type: file
+      description: STAR final log file
+      pattern: "*Log.final.out"
+  - log_out:
+      type: file
+      description: STAR lot out file
+      pattern: "*Log.out"
+  - log_progress:
+      type: file
+      description: STAR log progress file
+      pattern: "*Log.progress.out"
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+  - bam_sorted:
+      type: file
+      description: Sorted BAM file of read alignments (optional)
+      pattern: "*sortedByCoord.out.bam"
+  - bam_transcript:
+      type: file
+      description: Output BAM file of transcriptome alignment (optional)
+      pattern: "*toTranscriptome.out.bam"
+  - bam_unsorted:
+      type: file
+      description: Unsorted BAM file of read alignments (optional)
+      pattern: "*Aligned.unsort.out.bam"
+  - fastq:
+      type: file
+      description: Unmapped FastQ files (optional)
+      pattern: "*fastq.gz"
+  - tab:
+      type: file
+      description: STAR output tab file(s) (optional)
+      pattern: "*.tab"
+  - junction:
+      type: file
+      description: STAR chimeric junction output file (optional)
+      pattern: "*.out.junction"
+
+authors:
+  - "@kevinmenden"
+  - "@drpatelh"
+  - "@praveenraj2018"
diff --git a/modules/nf-core/modules/star/genomegenerate/functions.nf b/modules/nf-core/modules/star/genomegenerate/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/star/genomegenerate/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf
new file mode 100644
index 00000000..9335b9b5
--- /dev/null
+++ b/modules/nf-core/modules/star/genomegenerate/main.nf
@@ -0,0 +1,67 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process STAR_GENOMEGENERATE {
+    tag "$fasta"
+    label 'process_high'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) }
+
+    // Note: 2.7X indices incompatible with AWS iGenomes.
+    conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.13 conda-forge::gawk=5.1.0" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0"
+    } else {
+        container "quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0"
+    }
+
+    input:
+    path fasta
+    path gtf
+
+    output:
+    path "star"         , emit: index
+    path "*.version.txt", emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+    def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
+    def args     = options.args.tokenize()
+    if (args.contains('--genomeSAindexNbases')) {
+        """
+        mkdir star
+        STAR \\
+            --runMode genomeGenerate \\
+            --genomeDir star/ \\
+            --genomeFastaFiles $fasta \\
+            --sjdbGTFfile $gtf \\
+            --runThreadN $task.cpus \\
+            $memory \\
+            $options.args
+
+        STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt
+        """
+    } else {
+        """
+        samtools faidx $fasta
+        NUM_BASES=`gawk '{sum = sum + \$2}END{if ((log(sum)/log(2))/2 - 1 > 14) {printf "%.0f", 14} else {printf "%.0f", (log(sum)/log(2))/2 - 1}}' ${fasta}.fai`
+
+        mkdir star
+        STAR \\
+            --runMode genomeGenerate \\
+            --genomeDir star/ \\
+            --genomeFastaFiles $fasta \\
+            --sjdbGTFfile $gtf \\
+            --runThreadN $task.cpus \\
+            --genomeSAindexNbases \$NUM_BASES \\
+            $memory \\
+            $options.args
+
+        STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt
+        """
+    }
+}
diff --git a/modules/nf-core/modules/star/genomegenerate/meta.yml b/modules/nf-core/modules/star/genomegenerate/meta.yml
new file mode 100644
index 00000000..aae0219b
--- /dev/null
+++ b/modules/nf-core/modules/star/genomegenerate/meta.yml
@@ -0,0 +1,36 @@
+name: star_genomegenerate
+description: Create index for STAR
+keywords:
+  - index
+  - fasta
+  - genome
+  - reference
+tools:
+  - star:
+      description: |
+        STAR is a software package for mapping DNA sequences against
+        a large reference genome, such as the human genome.
+      homepage: https://github.com/alexdobin/STAR
+      manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
+      doi: 10.1093/bioinformatics/bts635
+input:
+  - fasta:
+      type: file
+      description: Fasta file of the reference genome
+  - gtf:
+      type: file
+      description: GTF file of the reference genome
+
+output:
+  - index:
+      type: directory
+      description: Folder containing the star index files
+      pattern: "star"
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+
+authors:
+  - "@kevinmenden"
+  - "@drpatelh"
diff --git a/modules/nf-core/modules/untar/functions.nf b/modules/nf-core/modules/untar/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/untar/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/untar/main.nf b/modules/nf-core/modules/untar/main.nf
new file mode 100644
index 00000000..fc6d7ec5
--- /dev/null
+++ b/modules/nf-core/modules/untar/main.nf
@@ -0,0 +1,35 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process UNTAR {
+    tag "$archive"
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img"
+    } else {
+        container "biocontainers/biocontainers:v1.2.0_cv1"
+    }
+
+    input:
+    path archive
+
+    output:
+    path "$untar"       , emit: untar
+    path "*.version.txt", emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+    untar        = archive.toString() - '.tar.gz'
+    """
+    tar -xzvf $options.args $archive
+    echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//' > ${software}.version.txt
+    """
+}
diff --git a/modules/nf-core/modules/untar/meta.yml b/modules/nf-core/modules/untar/meta.yml
new file mode 100644
index 00000000..af4674f0
--- /dev/null
+++ b/modules/nf-core/modules/untar/meta.yml
@@ -0,0 +1,27 @@
+name: untar
+description: Extract files.
+keywords:
+    - untar
+    - uncompress
+tools:
+    - untar:
+        description: |
+            Extract tar.gz files.
+        documentation: https://www.gnu.org/software/tar/manual/
+input:
+    - archive:
+        type: file
+        description: File to be untar
+        pattern: "*.{tar}.{gz}"
+output:
+    - untar:
+        type: file
+        description:
+        pattern: "*.*"
+    - version:
+        type: file
+        description: File containing software version
+        pattern: "*.{version.txt}"
+authors:
+    - "@joseespinosa"
+    - "@drpatelh"
diff --git a/nextflow.config b/nextflow.config
index 927863d6..34f35099 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -16,12 +16,19 @@ params {
     genome                     = null
     igenomes_base              = 's3://ngi-igenomes/igenomes'
     igenomes_ignore            = false
+    save_reference             = false
 
     // MultiQC options
     multiqc_config             = null
     multiqc_title              = null
     max_multiqc_email_size     = '25.MB'
 
+    //Alignment options
+    skip_alignment             = false
+    aligner                    = 'star'
+    star_ignore_sjdbgtf        = false
+    seq_center                 = null
+
     // Boilerplate options
     outdir                     = './results'
     tracedir                   = "${params.outdir}/pipeline_info"
diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf
new file mode 100644
index 00000000..ad7df07c
--- /dev/null
+++ b/subworkflows/local/prepare_genome.nf
@@ -0,0 +1,63 @@
+//
+// Uncompress and prepare reference genome files
+//
+
+params.genome_options       = [:]
+params.star_index_options   = [:]
+
+include { GUNZIP as GUNZIP_FASTA        } from '../../modules/nf-core/modules/gunzip/main'                  addParams( options: params.genome_options )
+include { UNTAR as UNTAR_STAR_INDEX     } from '../../modules/nf-core/modules/untar/main'                   addParams( options: params.star_index_options   )
+include { SAMTOOLS_FAIDX                } from '../../modules/nf-core/modules/samtools/faidx/main'          addParams( options: params.genome_options )
+include { STAR_GENOMEGENERATE           } from '../../modules/nf-core/modules/star/genomegenerate/main'     addParams( options: params.star_index_options )
+
+workflow PREPARE_GENOME {
+
+    take:
+    prepare_tool_indices
+
+    main:
+
+    // Uncompress genome fasta file if required
+    if (params.fasta.endsWith('.gz'))   ch_fasta = GUNZIP_FASTA ( params.fasta ).gunzipe
+    else                                ch_fasta = file(params.fasta)
+
+    // Index the genome fasta
+    ch_fasta_fai = Channel.empty()
+    if (params.fasta_fai)               ch_fasta_fai = file(params.fasta_fai)
+    else                                ch_fasta_fai = SAMTOOLS_FAIDX(ch_fasta).fai
+
+    // Get the GTF file
+    if (params.gtf) {
+        if (params.gtf.endsWith('.gz')) {
+            ch_gtf = GUNZIP_GTF ( params.gtf ).gunzip
+        } else {
+            ch_gtf = file(params.gtf)
+        }
+    }
+
+    // Uncompress STAR index or generate from scratch if required
+    ch_star_index   = Channel.empty()
+    ch_star_version = Channel.empty()
+    if ('star' in prepare_tool_indices) {
+        if (params.star_index) {
+            if (params.star_index.endsWith('.tar.gz')) {
+                ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar
+            } else {
+                ch_star_index = file(params.star_index)
+            }
+        } else {
+            ch_star_index   = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index
+            ch_star_version = STAR_GENOMEGENERATE.out.version
+        }
+    }
+
+    emit:
+    fasta            = ch_fasta            // path: genome.fasta
+    fai              = ch_fasta_fai        // path: genome.fasta.fai
+    gtf              = ch_gtf              // path: genome.gtf
+    star_index       = ch_star_index       // path: star/index/
+
+}
+
+
+
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 27173f52..edd7ac0a 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -20,6 +20,10 @@ for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true
 // Check mandatory parameters
 if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' }
 
+// Check alignment parameters
+def prepareToolIndices  = []
+if (!params.skip_alignment) { prepareToolIndices << params.aligner }
+
 /*
 ========================================================================================
     CONFIG FILES
@@ -37,8 +41,14 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 
 // Don't overwrite global params.modules, create a copy instead and use that within the main script.
 def modules = params.modules.clone()
+def publish_genome_options = params.save_reference ? [publish_dir: 'genome']       : [publish_files: false]
+def publish_index_options  = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false]
+def star_genomegenerate_options = modules['star_genomegenerate']
+if (!params.save_reference)     { star_genomegenerate_options['publish_files'] = false }
+
+def star_align_options = modules['star_align']
+def arriba_options     = modules['arriba_fusion']
 
-//
 // MODULE: Local to the pipeline
 //
 include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] )
@@ -46,8 +56,8 @@ include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions'
 //
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
-include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] )
-
+include { INPUT_CHECK } from '../subworkflows/local/input_check'                addParams( options: [:] )
+include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome'          addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options)
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
@@ -60,9 +70,10 @@ multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"
 //
 // MODULE: Installed directly from nf-core/modules
 //
-include { FASTQC  } from '../modules/nf-core/modules/fastqc/main'  addParams( options: modules['fastqc'] )
-include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options   )
-
+include { FASTQC  } from '../modules/nf-core/modules/fastqc/main'           addParams( options: modules['fastqc'] )
+include { MULTIQC } from '../modules/nf-core/modules/multiqc/main'          addParams( options: multiqc_options   )
+include { STAR_ALIGN } from '../modules/nf-core/modules/star/align/main'    addParams( options: star_align_options)
+include { ARRIBA } from '../modules/nf-core/modules/arriba/main'            addParams( options: arriba_options )
 /*
 ========================================================================================
     RUN MAIN WORKFLOW
@@ -74,20 +85,25 @@ def multiqc_report = []
 
 workflow RNAFUSION {
 
-    ch_software_versions = Channel.empty()
+    // SUBWORKFLOW: Uncompress and prepare reference genome files
+    PREPARE_GENOME (
+        prepareToolIndices
+    )
 
+    ch_software_versions = Channel.empty()
+    ch_reads = Channel.empty()
     //
     // SUBWORKFLOW: Read in samplesheet, validate and stage input files
     //
     INPUT_CHECK (
         ch_input
     )
-
+    ch_reads = INPUT_CHECK.out.reads
     //
     // MODULE: Run FastQC
     //
     FASTQC (
-        INPUT_CHECK.out.reads
+        ch_reads
     )
     ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null))
 
@@ -106,9 +122,8 @@ workflow RNAFUSION {
         ch_software_versions.map { it }.collect()
     )
 
-    //
     // MODULE: MultiQC
-    //
+
     workflow_summary    = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params)
     ch_workflow_summary = Channel.value(workflow_summary)
 
@@ -124,6 +139,28 @@ workflow RNAFUSION {
     )
     multiqc_report       = MULTIQC.out.report.toList()
     ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null))
+
+    // Run STAR alignment
+
+    ch_genome_bam        = Channel.empty()
+    ch_chimeric_junction = Channel.empty()
+    STAR_ALIGN (
+        ch_reads,
+        PREPARE_GENOME.out.star_index,
+        PREPARE_GENOME.out.gtf
+    )
+    ch_genome_bam        = STAR_ALIGN.out.bam
+
+    //Fusion calling with Arriba
+
+    ch_arriba_fusions = Channel.empty()
+    ARRIBA (
+        ch_genome_bam,
+        PREPARE_GENOME.out.fasta,
+        PREPARE_GENOME.out.gtf
+    )
+    ch_arriba_fusions = ARRIBA.out.fusions
+
 }
 
 /*

From a9eec60eb102a7596694874e69cd20de8e471ff2 Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Thu, 5 Aug 2021 11:01:35 +0200
Subject: [PATCH 036/359] Added fusioncatcher and starfusion as local modules

---
 modules/local/fusioncatcher/functions.nf      | 68 ++++++++++++++++
 modules/local/fusioncatcher/main.nf           | 62 +++++++++++++++
 modules/local/fusioncatcher/meta.yml          | 46 +++++++++++
 .../fusioncatcher_downloadgenome/functions.nf | 68 ++++++++++++++++
 .../fusioncatcher_downloadgenome/main.nf      | 77 +++++++++++++++++++
 .../fusioncatcher_downloadgenome/meta.yml     | 37 +++++++++
 modules/local/starfusion/functions.nf         | 68 ++++++++++++++++
 modules/local/starfusion/main.nf              | 54 +++++++++++++
 modules/local/starfusion/meta.yml             | 45 +++++++++++
 .../starfusion_downloadgenome/functions.nf    | 68 ++++++++++++++++
 .../local/starfusion_downloadgenome/main.nf   | 52 +++++++++++++
 .../local/starfusion_downloadgenome/meta.yml  | 27 +++++++
 12 files changed, 672 insertions(+)
 create mode 100644 modules/local/fusioncatcher/functions.nf
 create mode 100644 modules/local/fusioncatcher/main.nf
 create mode 100644 modules/local/fusioncatcher/meta.yml
 create mode 100644 modules/local/fusioncatcher_downloadgenome/functions.nf
 create mode 100644 modules/local/fusioncatcher_downloadgenome/main.nf
 create mode 100644 modules/local/fusioncatcher_downloadgenome/meta.yml
 create mode 100644 modules/local/starfusion/functions.nf
 create mode 100644 modules/local/starfusion/main.nf
 create mode 100644 modules/local/starfusion/meta.yml
 create mode 100644 modules/local/starfusion_downloadgenome/functions.nf
 create mode 100644 modules/local/starfusion_downloadgenome/main.nf
 create mode 100644 modules/local/starfusion_downloadgenome/meta.yml

diff --git a/modules/local/fusioncatcher/functions.nf b/modules/local/fusioncatcher/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/fusioncatcher/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/local/fusioncatcher/main.nf b/modules/local/fusioncatcher/main.nf
new file mode 100644
index 00000000..0cd4049b
--- /dev/null
+++ b/modules/local/fusioncatcher/main.nf
@@ -0,0 +1,62 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process FUSIONCATCHER {
+    tag "$meta.id"
+    label 'process_high'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+
+    conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_0"
+    } else {
+        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_0"
+    }
+
+    input:
+    tuple val(meta), path(reads)
+    path resource
+
+    output:
+    tuple val(meta), path("*.fusioncatcher-fusion-genes.txt")   , optional:true, emit: fusions
+    tuple val(meta), path("*.fusioncatcher_summary.txt")        , optional:true, emit: summary
+    tuple val(meta), path("*.fusioncatcher.log")                               , emit: log
+    path "*.version.txt"                                                       , emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def fastq    = meta.single_end ? "${reads[0]}" : "${reads[0]},${reads[1]}"
+    def valid    = resource.exists() && file("${resource}/organism.txt").exists()
+
+    if (valid) {
+
+        """
+        fusioncatcher \\
+            -d $resource \\
+            -i $fastq \\
+            -p $task.cpus \\
+            -o . \\
+            $options.args
+
+        mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher-fusion-genes.txt
+        mv summary_candidate_fusions.txt ${prefix}.fusioncatcher_summary.txt
+        mv fusioncatcher.log ${prefix}.fusioncatcher.log
+
+        fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
+        """
+
+    }
+    else{
+
+        """
+        echo "Error: Invalid genome database" > ${prefix}.fusioncatcher.log
+        fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
+        """
+    }
+}
diff --git a/modules/local/fusioncatcher/meta.yml b/modules/local/fusioncatcher/meta.yml
new file mode 100644
index 00000000..81efa6fd
--- /dev/null
+++ b/modules/local/fusioncatcher/meta.yml
@@ -0,0 +1,46 @@
+name: fusioncatcher
+description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
+keywords:
+  - fusioncatcher
+  - fusion
+tools:
+  - fusioncatcher:
+      description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
+      homepage: https://github.com/ndaniel/fusioncatcher
+      documentation: https://github.com/ndaniel/fusioncatcher/wiki
+      tool_dev_url: https://github.com/ndaniel/fusioncatcher
+      doi: "10.1101/011650v1"
+      licence: ['GPL v3']
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: FASTQ file
+      pattern: "*.{fastq}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+  - fusions:
+      type: file
+      description: Final list of candidate fusion genes
+      pattern: "*.{fusioncatcher.txt}"
+  - bam:
+      type: file
+      description: Summary of fusion results
+      pattern: "*.{fusioncatcher_summary.txt}"
+
+authors:
+  - "@praveenraj2018"
diff --git a/modules/local/fusioncatcher_downloadgenome/functions.nf b/modules/local/fusioncatcher_downloadgenome/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/fusioncatcher_downloadgenome/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/local/fusioncatcher_downloadgenome/main.nf b/modules/local/fusioncatcher_downloadgenome/main.nf
new file mode 100644
index 00000000..a71b4709
--- /dev/null
+++ b/modules/local/fusioncatcher_downloadgenome/main.nf
@@ -0,0 +1,77 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+// TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :)
+//               https://github.com/nf-core/modules/tree/master/software
+//               You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace:
+//               https://nf-co.re/join
+
+// TODO nf-core: A module file SHOULD only define input and output files as command-line parameters.
+//               All other parameters MUST be provided as a string i.e. "options.args"
+//               where "params.options" is a Groovy Map that MUST be provided via the addParams section of the including workflow.
+//               Any parameters that need to be evaluated in the context of a particular sample
+//               e.g. single-end/paired-end data MUST also be defined and evaluated appropriately.
+// TODO nf-core: Software that can be piped together SHOULD be added to separate module files
+//               unless there is a run-time, storage advantage in implementing in this way
+//               e.g. it's ok to have a single module for bwa to output BAM instead of SAM:
+//                 bwa mem | samtools view -B -T ref.fasta
+// TODO nf-core: Optional inputs are not currently supported by Nextflow. However, using an empty
+//               list (`[]`) instead of a file can be used to work around this issue.
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process FUSIONCATCHER_DOWNLOADGENOME {
+    tag '$bam'
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    // TODO nf-core: List required Conda package(s).
+    //               Software MUST be pinned to channel (i.e. "bioconda"), version (i.e. "1.10").
+    //               For Conda, the build (i.e. "h9402c20_2") must be EXCLUDED to support installation on different operating systems.
+    // TODO nf-core: See section in main README for further information regarding finding and adding container addresses to the section below.
+    conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_0"
+    } else {
+        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_0"
+    }
+
+    input:
+    // TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group"
+    //               MUST be provided as an input via a Groovy Map called "meta".
+    //               This information may not be required in some instances e.g. indexing reference genome files:
+    //               https://github.com/nf-core/modules/blob/master/software/bwa/index/main.nf
+    // TODO nf-core: Where applicable please provide/convert compressed files as input/output
+    //               e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc.
+    path bam
+
+    output:
+    // TODO nf-core: Named file extensions MUST be emitted for ALL output channels
+    path "*.bam", emit: bam
+    // TODO nf-core: List additional required output channels/values here
+    path "*.version.txt"          , emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+    
+    // TODO nf-core: Where possible, a command MUST be provided to obtain the version number of the software e.g. 1.10
+    //               If the software is unable to output a version number on the command-line then it can be manually specified
+    //               e.g. https://github.com/nf-core/modules/blob/master/software/homer/annotatepeaks/main.nf
+    // TODO nf-core: It MUST be possible to pass additional parameters to the tool as a command-line string via the "$options.args" variable
+    // TODO nf-core: If the tool supports multi-threading then you MUST provide the appropriate parameter
+    //               using the Nextflow "task" variable e.g. "--threads $task.cpus"
+    // TODO nf-core: Please replace the example samtools command below with your module's command
+    // TODO nf-core: Please indent the command appropriately (4 spaces!!) to help with readability ;)
+    """
+    samtools \\
+        sort \\
+        $options.args \\
+        -@ $task.cpus \\
+        $bam
+
+    echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt
+    """
+}
diff --git a/modules/local/fusioncatcher_downloadgenome/meta.yml b/modules/local/fusioncatcher_downloadgenome/meta.yml
new file mode 100644
index 00000000..050e987b
--- /dev/null
+++ b/modules/local/fusioncatcher_downloadgenome/meta.yml
@@ -0,0 +1,37 @@
+name: fusioncatcher_downloadgenome
+## TODO nf-core: Add a description of the module and list keywords
+description: write your description here
+keywords:
+  - sort
+tools:
+  - fusioncatcher:
+      ## TODO nf-core: Add a description and other details for the software below
+      description: Finder of Somatic Fusion Genes in RNA-seq data.
+      homepage: None
+      documentation: None
+      tool_dev_url: None
+      doi: ""
+      licence: ['GPL v3']
+
+## TODO nf-core: Add a description of all of the variables used as input
+input:
+  ## TODO nf-core: Delete / customise this example input
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+
+## TODO nf-core: Add a description of all of the variables used as output
+output:
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+  ## TODO nf-core: Delete / customise this example output
+  - bam:
+      type: file
+      description: Sorted BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+
+authors:
+  - "@praveenraj2018"
diff --git a/modules/local/starfusion/functions.nf b/modules/local/starfusion/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/starfusion/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/local/starfusion/main.nf b/modules/local/starfusion/main.nf
new file mode 100644
index 00000000..938ad7b8
--- /dev/null
+++ b/modules/local/starfusion/main.nf
@@ -0,0 +1,54 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process STARFUSION {
+    tag "$meta.id"
+    label 'process_medium'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+
+    conda (params.enable_conda ? "bioconda::star-fusion=1.10.0" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/star-fusion:1.10.0--hdfd78af_1"
+    } else {
+        container "quay.io/biocontainers/star-fusion:1.10.0--hdfd78af_1"
+    }
+
+    input:
+    tuple val(meta), path(junction)
+    path genome_resource_lib
+
+    output:
+    tuple val(meta), path("*.fusion_predictions.tsv")   , optional:true, emit: fusions
+    path "*.version.txt"                                , emit: version
+
+    script:
+    def software            = getSoftwareName(task.process)
+    def prefix              = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def genome_resource     = (genome_resource_lib.exists() && file("${genome_resource_lib}/AnnotFilterRule.pm").exists()) ? true : false
+
+    if (genome_resource) {
+
+        """
+        STAR-Fusion --genome_lib_dir $genome_resource_lib \\
+            -J $junction \\
+            --CPU $task.cpus
+            --examine_coding_effect \\
+            --output_dir . \\
+            $options.args
+
+        echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > ${software}.version.txt
+        """
+    }
+    else{
+
+        """
+        echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > ${software}.version.txt
+        """
+
+    }
+}
diff --git a/modules/local/starfusion/meta.yml b/modules/local/starfusion/meta.yml
new file mode 100644
index 00000000..2c38cd1a
--- /dev/null
+++ b/modules/local/starfusion/meta.yml
@@ -0,0 +1,45 @@
+name: starfusion
+description: Fast and Accurate Fusion Transcript Detection from RNA-Seq
+keywords:
+  - Fusion
+tools:
+  - star-fusion:
+      description: Fast and Accurate Fusion Transcript Detection from RNA-Seq
+      homepage: https://github.com/STAR-Fusion/STAR-Fusion
+      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki
+      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion/releases
+      doi: "10.1101/120295v1"
+      licence: ['GPL v3']
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - junction:
+      type: file
+      description: Chimeric junction output from STAR aligner
+      pattern: "*.{out.junction}"
+  - genome_lib:
+      type: file
+      description: STAR-fusion reference genome lib folder
+      pattern: "*.*"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+  - fusions:
+      type: file
+      description: Fusion events from STAR-fusion
+      pattern: "*.{fusion_predictions.tsv}"
+
+authors:
+  - "@praveenraj2018"
diff --git a/modules/local/starfusion_downloadgenome/functions.nf b/modules/local/starfusion_downloadgenome/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/starfusion_downloadgenome/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/local/starfusion_downloadgenome/main.nf b/modules/local/starfusion_downloadgenome/main.nf
new file mode 100644
index 00000000..a0fbf06b
--- /dev/null
+++ b/modules/local/starfusion_downloadgenome/main.nf
@@ -0,0 +1,52 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process STARFUSION_DOWNLOADGENOME {
+    tag 'genome_build'
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'resources', meta:[:], publish_by_meta:[]) }
+
+    input:
+    val genome_build
+
+    output:
+    path "star-fusion-genome"       , emit: reference
+
+    script:
+    def software        = getSoftwareName(task.process)
+
+    if ( genome_build == "GRCh37" ){
+
+    """
+    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play.tar.gz .
+    mkdir -p star-fusion-genome
+    tar -zxf GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play.tar.gz --strip-components=2 -C star-fusion-genome/
+    rm GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play.tar.gz
+    """
+
+    }
+    else if (genome_build == "GRCh38"){
+
+    """
+    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz .
+    mkdir -p star-fusion-genome
+    tar -zxf GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --strip-components=2 -C star-fusion-genome/
+    rm GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz
+    """
+
+    }
+    else{
+
+    """
+    mkdir -p star-fusion-genome
+    echo "Unsupported genome build" > error.log
+    mv error.log star-fusion-genome/
+    """
+
+    }
+}
diff --git a/modules/local/starfusion_downloadgenome/meta.yml b/modules/local/starfusion_downloadgenome/meta.yml
new file mode 100644
index 00000000..91e3ecdc
--- /dev/null
+++ b/modules/local/starfusion_downloadgenome/meta.yml
@@ -0,0 +1,27 @@
+name: starfusion_downloadgenome
+description: Download STAR-fusion genome resource required to run STAR-Fusion caller
+keywords:
+  - downoad
+tools:
+  - star-fusion:
+      description: Fusion calling algorithm for RNAseq data
+      homepage: https://github.com/STAR-Fusion/
+      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki/installing-star-fusion
+      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion
+      doi: "10.1186/s13059-019-1842-9"
+      licence: ['GPL v3']
+
+input:
+  - genome_version:
+      type: value
+      description: Genome version - either GRCh37 or GRCh38
+      pattern: "GRCh3*"
+
+output:
+  - reference:
+      type: file
+      description: Genome resource path
+      pattern: "*"
+
+authors:
+  - "@praveenraj2018"

From bda5a2ffd084d562a374ad0c0c86792c7b9f09ac Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Thu, 5 Aug 2021 15:46:06 +0200
Subject: [PATCH 037/359] Add star-arriba subworkflow and tested

---
 conf/modules.config                        |  6 +--
 subworkflows/nf-core/fusion_star_arriba.nf | 50 ++++++++++++++++++++++
 workflows/rnafusion.nf                     | 32 ++++----------
 3 files changed, 62 insertions(+), 26 deletions(-)
 create mode 100644 subworkflows/nf-core/fusion_star_arriba.nf

diff --git a/conf/modules.config b/conf/modules.config
index 0c4073de..72119c17 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -34,13 +34,13 @@ params {
         }
         'star_align' {
             args            = "--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
-            publish_dir     = "${params.aligner}"
+            publish_dir     = "alignment/${params.aligner}"
             publish_files   = ['bam':'', 'junction':'', 'out':'log', 'tab':'log']
         }
         'arriba_fusion' {
             args            = ""
-            publish_dir     = 'arriba'
-            publish_files   = ['fusions':'', 'fusions_fail':'']
+            publish_dir     = 'fusions/arriba'
+            publish_files   = ['tsv':'']
         }
     }
 }
diff --git a/subworkflows/nf-core/fusion_star_arriba.nf b/subworkflows/nf-core/fusion_star_arriba.nf
new file mode 100644
index 00000000..3c9da480
--- /dev/null
+++ b/subworkflows/nf-core/fusion_star_arriba.nf
@@ -0,0 +1,50 @@
+/*
+ * Fusion calling with STAR and Arriba
+ */
+
+params.star_align_options = [:]
+params.arriba_options = [:]
+
+include { STAR_ALIGN }  from '../../modules/nf-core/modules/star/align/main.nf'    addParams( options: params.star_align_options)
+include { ARRIBA }      from '../../modules/nf-core/modules/arriba/main.nf'        addParams( options: params.arriba_options )
+
+workflow FUSION_STAR_ARRIBA {
+    take:
+    reads           // channel: [ val(meta), [ reads ] ]
+    star_index      // channel: STAR index
+    genome_fasta    // channel: genome FASTA file
+    genome_gtf      // channel: genome GTF file
+
+    main:
+
+    //RNAseq alignment with STAR
+    ch_genome_bam = Channel.empty()
+    ch_star_version = Channel.empty()
+    STAR_ALIGN (
+        reads,
+        star_index,
+        genome_gtf
+    )
+    ch_genome_bam   = STAR_ALIGN.out.bam
+    ch_star_version = STAR_ALIGN.out.version
+
+    //Fusion calling with Arriba
+    ch_arriba_fusions        = Channel.empty()
+    ch_arriba_failed_fusions = Channel.empty()
+    ch_arriba_version        = Channel.empty()
+    ARRIBA (
+        ch_genome_bam,
+        genome_fasta,
+        genome_gtf
+    )
+    ch_arriba_fusions        = ARRIBA.out.fusions
+    ch_arriba_failed_fusions = ARRIBA.out.fusions_fail
+    ch_arriba_version        = ARRIBA.out.version
+
+    emit:
+    bam          = ch_genome_bam            // channel: [ val(meta), [ bam ] ]
+    fusions      = ch_arriba_fusions        // channel: [ val(meta), [ fusions ]
+    fusions_fail = ch_arriba_failed_fusions // channel: [ val(meta), [ fusions_fail ]
+    ch_star_version
+    ch_arriba_version
+}
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index edd7ac0a..586102bc 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -46,13 +46,9 @@ def publish_index_options  = params.save_reference ? [publish_dir: 'genome/index
 def star_genomegenerate_options = modules['star_genomegenerate']
 if (!params.save_reference)     { star_genomegenerate_options['publish_files'] = false }
 
-def star_align_options = modules['star_align']
-def arriba_options     = modules['arriba_fusion']
-
 // MODULE: Local to the pipeline
 //
 include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] )
-
 //
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
@@ -70,10 +66,9 @@ multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"
 //
 // MODULE: Installed directly from nf-core/modules
 //
-include { FASTQC  } from '../modules/nf-core/modules/fastqc/main'           addParams( options: modules['fastqc'] )
-include { MULTIQC } from '../modules/nf-core/modules/multiqc/main'          addParams( options: multiqc_options   )
-include { STAR_ALIGN } from '../modules/nf-core/modules/star/align/main'    addParams( options: star_align_options)
-include { ARRIBA } from '../modules/nf-core/modules/arriba/main'            addParams( options: arriba_options )
+include { FASTQC  }             from '../modules/nf-core/modules/fastqc/main'           addParams( options: modules['fastqc'] )
+include { MULTIQC }             from '../modules/nf-core/modules/multiqc/main'          addParams( options: multiqc_options   )
+include { FUSION_STAR_ARRIBA }  from '../subworkflows/nf-core/fusion_star_arriba'       addParams( star_align_options: modules['star_align'], arriba_options: modules['arriba_fusion'])
 /*
 ========================================================================================
     RUN MAIN WORKFLOW
@@ -140,26 +135,17 @@ workflow RNAFUSION {
     multiqc_report       = MULTIQC.out.report.toList()
     ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null))
 
-    // Run STAR alignment
-
-    ch_genome_bam        = Channel.empty()
-    ch_chimeric_junction = Channel.empty()
-    STAR_ALIGN (
+    // Run STAR alignment and Arriba
+    ch_genome_bam     = Channel.empty()
+    ch_arriba_fusions = Channel.empty()
+    FUSION_STAR_ARRIBA (
         ch_reads,
         PREPARE_GENOME.out.star_index,
-        PREPARE_GENOME.out.gtf
-    )
-    ch_genome_bam        = STAR_ALIGN.out.bam
-
-    //Fusion calling with Arriba
-
-    ch_arriba_fusions = Channel.empty()
-    ARRIBA (
-        ch_genome_bam,
         PREPARE_GENOME.out.fasta,
         PREPARE_GENOME.out.gtf
     )
-    ch_arriba_fusions = ARRIBA.out.fusions
+    ch_genome_bam     = FUSION_STAR_ARRIBA.out.bam
+    ch_arriba_fusions = FUSION_STAR_ARRIBA.out.fusions
 
 }
 

From 00bca8bba9d88d97ad6520fabbe50bda4fcc27ad Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Thu, 5 Aug 2021 16:14:46 +0200
Subject: [PATCH 038/359] Fix: lint errors

---
 modules.json                               |  2 +-
 modules/nf-core/modules/star/align/main.nf | 11 ++++++-----
 nextflow.config                            |  4 +---
 nextflow_schema.json                       | 21 +++++++++++++++++++++
 4 files changed, 29 insertions(+), 9 deletions(-)

diff --git a/modules.json b/modules.json
index c20f9732..2c4dfa15 100644
--- a/modules.json
+++ b/modules.json
@@ -19,7 +19,7 @@
                 "git_sha": "c5235a983d454787fa0c3247b02086969217163b"
             },
             "star/align": {
-                "git_sha": "df909015b6651dadb52626cf471f15a6d33985a2"
+                "git_sha": "5de3f2c50e063bda30316342b6606b485a12e38c"
             },
             "star/genomegenerate": {
                 "git_sha": "f43778b0e641a9643ae73fe1f2fe2d5bb81edffc"
diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf
index c06daf24..d5d88ce8 100644
--- a/modules/nf-core/modules/star/align/main.nf
+++ b/modules/nf-core/modules/star/align/main.nf
@@ -39,11 +39,12 @@ process STAR_ALIGN {
     tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
 
     script:
-    def software   = getSoftwareName(task.process)
-    def prefix     = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
-    def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix'"
-    def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
+    def software        = getSoftwareName(task.process)
+    def prefix          = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def ignore_gtf      = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
+    def seq_platform    = params.seq_platform ? "'PL:$params.seq_platform'" : ""
+    def seq_center      = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform "
+    def out_sam_type    = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
     def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
     """
     STAR \\
diff --git a/nextflow.config b/nextflow.config
index 34f35099..6091e057 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -24,10 +24,8 @@ params {
     max_multiqc_email_size     = '25.MB'
 
     //Alignment options
-    skip_alignment             = false
-    aligner                    = 'star'
-    star_ignore_sjdbgtf        = false
     seq_center                 = null
+    seq_platform               = null
 
     // Boilerplate options
     outdir                     = './results'
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 2f692042..a9fe439e 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -267,6 +267,27 @@
                     "hidden": true,
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues."
+                },
+                "save_reference": {
+                    "type": "boolean",
+                    "description": "Whether to save the reference or not.",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Save the reference for future executions."
+                },
+                "seq_center": {
+                    "type": "boolean",
+                    "description": "Sequencing center",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "This will reported in the BAM header as CN"
+                },
+                "seq_platform": {
+                    "type": "boolean",
+                    "description": "Sequencing platform",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "This will reported in the BAM header as PL."
                 }
             }
         }

From 9efe30beacfec23cc037fed819b4f231c6adb27d Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Mon, 9 Aug 2021 15:29:42 +0200
Subject: [PATCH 039/359] Added star-fusion as local module into pipeline

---
 conf/modules.config                  |  5 ++++
 conf/test.config                     |  3 +++
 modules/local/starfusion/main.nf     | 13 +++++-----
 nextflow.config                      | 14 ++++++++++-
 nextflow_schema.json                 | 14 +++++++++++
 subworkflows/local/prepare_genome.nf | 23 ++++++++++++++----
 workflows/rnafusion.nf               | 36 ++++++++++++++++++----------
 7 files changed, 84 insertions(+), 24 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 72119c17..1a9d7cc8 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -42,5 +42,10 @@ params {
             publish_dir     = 'fusions/arriba'
             publish_files   = ['tsv':'']
         }
+        'starfusion' {
+            args            = "--examine_coding_effect --STAR_use_shared_memory"
+            publish_dir     = 'fusions/starfusion'
+            publish_files   = ['tsv':'']
+        }
     }
 }
diff --git a/conf/test.config b/conf/test.config
index f5ed8827..661fd0d9 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -29,4 +29,7 @@ params {
 
     //STAR index
     star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
+
+    //STAR-Fusion
+    starfusion_genome   = '/hdd2/genome_builds/star_fusion/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir'
 }
diff --git a/modules/local/starfusion/main.nf b/modules/local/starfusion/main.nf
index 938ad7b8..b55de370 100644
--- a/modules/local/starfusion/main.nf
+++ b/modules/local/starfusion/main.nf
@@ -6,7 +6,7 @@ options        = initOptions(params.options)
 
 process STARFUSION {
     tag "$meta.id"
-    label 'process_medium'
+    label 'process_high'
     publishDir "${params.outdir}",
         mode: params.publish_dir_mode,
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
@@ -19,25 +19,24 @@ process STARFUSION {
     }
 
     input:
-    tuple val(meta), path(junction)
+    tuple val(meta), path(reads)
     path genome_resource_lib
 
     output:
-    tuple val(meta), path("*.fusion_predictions.tsv")   , optional:true, emit: fusions
+    tuple val(meta), path("*.fusion_predictions.tsv")   , emit: fusions
     path "*.version.txt"                                , emit: version
 
     script:
     def software            = getSoftwareName(task.process)
     def prefix              = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def genome_resource     = (genome_resource_lib.exists() && file("${genome_resource_lib}/AnnotFilterRule.pm").exists()) ? true : false
+    def fastq               = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
+    def genome_resource     = true //genome_resource_lib.exists() && file("${genome_resource_lib}/AnnotFilterRule.pm").exists()
 
     if (genome_resource) {
 
         """
         STAR-Fusion --genome_lib_dir $genome_resource_lib \\
-            -J $junction \\
-            --CPU $task.cpus
-            --examine_coding_effect \\
+            $fastq \\
             --output_dir . \\
             $options.args
 
diff --git a/nextflow.config b/nextflow.config
index 6091e057..3e2d65fe 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -13,10 +13,11 @@ params {
     input                      = null
 
     // References
-    genome                     = null
+    genome                     = 'GRCh38'
     igenomes_base              = 's3://ngi-igenomes/igenomes'
     igenomes_ignore            = false
     save_reference             = false
+    aligner                    = 'star'
 
     // MultiQC options
     multiqc_config             = null
@@ -24,9 +25,20 @@ params {
     max_multiqc_email_size     = '25.MB'
 
     //Alignment options
+    star_ignore_sjdbgtf        = false
     seq_center                 = null
     seq_platform               = null
 
+    //Enable or disable tools
+    arriba                     = true
+    starfusion                 = false
+    fusioncatcher              = false
+    ericscript                 = false
+    squid                      = false
+    pizzly                     = false
+
+
+
     // Boilerplate options
     outdir                     = './results'
     tracedir                   = "${params.outdir}/pipeline_info"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index a9fe439e..9b68bb47 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -288,6 +288,20 @@
                     "hidden": true,
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "This will reported in the BAM header as PL."
+                },
+                "aligner": {
+                    "type": "string",
+                    "description": "List of read aligners to be used",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Specify the aligned to be used for RNAseq alignment"
+                },
+                "star_ignore_sjdbgtf": {
+                    "type": "boolean",
+                    "description": "Whether to ignore the GTF in STAR alignment",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Setting false will use GTF file for STAR alignment"
                 }
             }
         }
diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf
index ad7df07c..b2821e41 100644
--- a/subworkflows/local/prepare_genome.nf
+++ b/subworkflows/local/prepare_genome.nf
@@ -51,11 +51,26 @@ workflow PREPARE_GENOME {
         }
     }
 
+    //Check genome data required for STAR-Fusion
+    ch_starfusion_resource = Channel.empty()
+    if (params.starfusion){
+        if (params.starfusion_genome) {
+            ch_sf_genome = params.starfusion_genome
+            if(file("${ch_sf_genome}/AnnotFilterRule.pm").exists()){
+                ch_starfusion_resource = file(ch_sf_genome)
+            }
+        }
+        else{
+            ch_starfusion_resource = STARFUSION_DOWNLOADGENOME (params.genome).out.reference
+        }
+    }
+
     emit:
-    fasta            = ch_fasta            // path: genome.fasta
-    fai              = ch_fasta_fai        // path: genome.fasta.fai
-    gtf              = ch_gtf              // path: genome.gtf
-    star_index       = ch_star_index       // path: star/index/
+    fasta               = ch_fasta            // path: genome.fasta
+    fai                 = ch_fasta_fai        // path: genome.fasta.fai
+    gtf                 = ch_gtf              // path: genome.gtf
+    star_index          = ch_star_index       // path: star/index/
+    starfusion_resource = ch_starfusion_resource
 
 }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 586102bc..8b529a2f 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -22,7 +22,7 @@ if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input sample
 
 // Check alignment parameters
 def prepareToolIndices  = []
-if (!params.skip_alignment) { prepareToolIndices << params.aligner }
+prepareToolIndices << params.aligner
 
 /*
 ========================================================================================
@@ -69,6 +69,8 @@ multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"
 include { FASTQC  }             from '../modules/nf-core/modules/fastqc/main'           addParams( options: modules['fastqc'] )
 include { MULTIQC }             from '../modules/nf-core/modules/multiqc/main'          addParams( options: multiqc_options   )
 include { FUSION_STAR_ARRIBA }  from '../subworkflows/nf-core/fusion_star_arriba'       addParams( star_align_options: modules['star_align'], arriba_options: modules['arriba_fusion'])
+include { STARFUSION }          from '../modules/local/starfusion/main'         addParams( options: modules['starfusion'] )
+
 /*
 ========================================================================================
     RUN MAIN WORKFLOW
@@ -136,17 +138,27 @@ workflow RNAFUSION {
     ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null))
 
     // Run STAR alignment and Arriba
-    ch_genome_bam     = Channel.empty()
-    ch_arriba_fusions = Channel.empty()
-    FUSION_STAR_ARRIBA (
-        ch_reads,
-        PREPARE_GENOME.out.star_index,
-        PREPARE_GENOME.out.fasta,
-        PREPARE_GENOME.out.gtf
-    )
-    ch_genome_bam     = FUSION_STAR_ARRIBA.out.bam
-    ch_arriba_fusions = FUSION_STAR_ARRIBA.out.fusions
-
+    if (params.arriba){
+        ch_genome_bam     = Channel.empty()
+        ch_arriba_fusions = Channel.empty()
+        FUSION_STAR_ARRIBA (
+            ch_reads,
+            PREPARE_GENOME.out.star_index,
+            PREPARE_GENOME.out.fasta,
+            PREPARE_GENOME.out.gtf
+        )
+        ch_genome_bam     = FUSION_STAR_ARRIBA.out.bam
+        ch_arriba_fusions = FUSION_STAR_ARRIBA.out.fusions
+    }
+
+    //Run STAR fusion
+    if (params.starfusion){
+        ch_star_fusions = Channel.empty()
+        STARFUSION (
+            ch_reads,
+            PREPARE_GENOME.out.starfusion_resource
+        )
+    }
 }
 
 /*

From 252772f9cd6090239fd3e36c5a0e319a316e248a Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Tue, 10 Aug 2021 15:08:22 +0200
Subject: [PATCH 040/359] fix: bugs

---
 CHANGELOG.md                                  |  2 ++
 conf/modules.config                           | 10 +++++++---
 conf/test.config                              |  3 +--
 modules.json                                  |  2 +-
 .../local/starfusion_downloadgenome/main.nf   |  5 +++--
 nextflow.config                               |  2 --
 subworkflows/local/prepare_genome.nf          | 19 ++++++++++++-------
 workflows/rnafusion.nf                        |  5 +++--
 8 files changed, 29 insertions(+), 19 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3a8b114f..e4e481eb 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -8,6 +8,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
 
 ### `Added`
+1. Added STAR Alignment followed by Arriba fusion calling
+2. Added STAR Fusion calling
 
 ### `Fixed`
 
diff --git a/conf/modules.config b/conf/modules.config
index 1a9d7cc8..df3ed1f1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -29,8 +29,12 @@ params {
             args = ""
         }
        'star_genomegenerate' {
-            args             = ""
-            publish_dir      = 'genome/index'
+            args            = ""
+            publish_dir     = 'genome/index'
+        }
+        'starfusion_genomegenerate' {
+            args            = "--strip-components=2"
+            publish_dir     = 'genome/starfusion_resource'
         }
         'star_align' {
             args            = "--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
@@ -43,7 +47,7 @@ params {
             publish_files   = ['tsv':'']
         }
         'starfusion' {
-            args            = "--examine_coding_effect --STAR_use_shared_memory"
+            args            = "--examine_coding_effect"
             publish_dir     = 'fusions/starfusion'
             publish_files   = ['tsv':'']
         }
diff --git a/conf/test.config b/conf/test.config
index 661fd0d9..4808bd5f 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -31,5 +31,4 @@ params {
     star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
 
     //STAR-Fusion
-    starfusion_genome   = '/hdd2/genome_builds/star_fusion/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir'
-}
+    starfusion_genome   = 'https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.10/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz'
diff --git a/modules.json b/modules.json
index 2c4dfa15..802ee198 100644
--- a/modules.json
+++ b/modules.json
@@ -29,4 +29,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/local/starfusion_downloadgenome/main.nf b/modules/local/starfusion_downloadgenome/main.nf
index a0fbf06b..46fa3a17 100644
--- a/modules/local/starfusion_downloadgenome/main.nf
+++ b/modules/local/starfusion_downloadgenome/main.nf
@@ -15,10 +15,10 @@ process STARFUSION_DOWNLOADGENOME {
     val genome_build
 
     output:
-    path "star-fusion-genome"       , emit: reference
+    path "star-fusion-genome"   , emit: reference
 
     script:
-    def software        = getSoftwareName(task.process)
+    def software = getSoftwareName(task.process)
 
     if ( genome_build == "GRCh37" ){
 
@@ -49,4 +49,5 @@ process STARFUSION_DOWNLOADGENOME {
     """
 
     }
+
 }
diff --git a/nextflow.config b/nextflow.config
index 3e2d65fe..c19ce907 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -37,8 +37,6 @@ params {
     squid                      = false
     pizzly                     = false
 
-
-
     // Boilerplate options
     outdir                     = './results'
     tracedir                   = "${params.outdir}/pipeline_info"
diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf
index b2821e41..85d4c775 100644
--- a/subworkflows/local/prepare_genome.nf
+++ b/subworkflows/local/prepare_genome.nf
@@ -2,13 +2,15 @@
 // Uncompress and prepare reference genome files
 //
 
-params.genome_options       = [:]
-params.star_index_options   = [:]
+params.genome_options               = [:]
+params.star_index_options           = [:]
+params.starfusion_genome_options    = [:]
 
-include { GUNZIP as GUNZIP_FASTA        } from '../../modules/nf-core/modules/gunzip/main'                  addParams( options: params.genome_options )
-include { UNTAR as UNTAR_STAR_INDEX     } from '../../modules/nf-core/modules/untar/main'                   addParams( options: params.star_index_options   )
-include { SAMTOOLS_FAIDX                } from '../../modules/nf-core/modules/samtools/faidx/main'          addParams( options: params.genome_options )
-include { STAR_GENOMEGENERATE           } from '../../modules/nf-core/modules/star/genomegenerate/main'     addParams( options: params.star_index_options )
+include { GUNZIP as GUNZIP_FASTA                } from '../../modules/nf-core/modules/gunzip/main'                  addParams( options: params.genome_options )
+include { UNTAR as UNTAR_STAR_INDEX             } from '../../modules/nf-core/modules/untar/main'                   addParams( options: params.star_index_options )
+include { UNTAR as UNTAR_STARFUSION_GENOME      } from '../../modules/nf-core/modules/untar/main'                   addParams( options: params.starfusion_genome_options )
+include { SAMTOOLS_FAIDX                        } from '../../modules/nf-core/modules/samtools/faidx/main'          addParams( options: params.genome_options )
+include { STAR_GENOMEGENERATE                   } from '../../modules/nf-core/modules/star/genomegenerate/main'     addParams( options: params.star_index_options )
 
 workflow PREPARE_GENOME {
 
@@ -56,7 +58,10 @@ workflow PREPARE_GENOME {
     if (params.starfusion){
         if (params.starfusion_genome) {
             ch_sf_genome = params.starfusion_genome
-            if(file("${ch_sf_genome}/AnnotFilterRule.pm").exists()){
+            if (ch_sf_genome.endsWith('.tar.gz')){
+                ch_starfusion_resource = UNTAR_STARFUSION_GENOME (ch_sf_genome).untar
+            }
+            else if(file("${ch_sf_genome}/AnnotFilterRule.pm").exists()){
                 ch_starfusion_resource = file(ch_sf_genome)
             }
         }
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 8b529a2f..15de3b41 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -44,6 +44,7 @@ def modules = params.modules.clone()
 def publish_genome_options = params.save_reference ? [publish_dir: 'genome']       : [publish_files: false]
 def publish_index_options  = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false]
 def star_genomegenerate_options = modules['star_genomegenerate']
+def starfusion_genome_options = modules['starfusion_genomegenerate']
 if (!params.save_reference)     { star_genomegenerate_options['publish_files'] = false }
 
 // MODULE: Local to the pipeline
@@ -53,7 +54,7 @@ include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions'
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
 include { INPUT_CHECK } from '../subworkflows/local/input_check'                addParams( options: [:] )
-include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome'          addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options)
+include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome'          addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options, starfusion_genome_options: starfusion_genome_options)
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
@@ -69,7 +70,7 @@ multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"
 include { FASTQC  }             from '../modules/nf-core/modules/fastqc/main'           addParams( options: modules['fastqc'] )
 include { MULTIQC }             from '../modules/nf-core/modules/multiqc/main'          addParams( options: multiqc_options   )
 include { FUSION_STAR_ARRIBA }  from '../subworkflows/nf-core/fusion_star_arriba'       addParams( star_align_options: modules['star_align'], arriba_options: modules['arriba_fusion'])
-include { STARFUSION }          from '../modules/local/starfusion/main'         addParams( options: modules['starfusion'] )
+include { STARFUSION }          from '../modules/local/starfusion/main'                 addParams( options: modules['starfusion'] )
 
 /*
 ========================================================================================

From 96396b0df56e5be729c00f218a231545db35558c Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Wed, 11 Aug 2021 15:28:39 +0200
Subject: [PATCH 041/359] Fix: PR review changes

---
 .github/workflows/awsfulltest.yml             |  5 +-
 conf/modules.config                           | 19 +++--
 conf/test.config                              |  3 +-
 docs/output.md                                |  2 -
 docs/usage.md                                 |  4 +-
 lib/WorkflowMain.groovy                       |  1 -
 .../{ => detection}/functions.nf              |  0
 .../fusioncatcher/{ => detection}/main.nf     | 39 ++++------
 .../fusioncatcher/{ => detection}/meta.yml    |  0
 .../download}/functions.nf                    |  0
 modules/local/fusioncatcher/download/main.nf  | 39 ++++++++++
 modules/local/fusioncatcher/download/meta.yml | 31 ++++++++
 .../fusioncatcher_downloadgenome/main.nf      | 77 -------------------
 .../fusioncatcher_downloadgenome/meta.yml     | 37 ---------
 .../starfusion/{ => detection}/functions.nf   |  0
 .../local/starfusion/{ => detection}/main.nf  | 36 ++++-----
 .../local/starfusion/{ => detection}/meta.yml |  0
 .../download}/functions.nf                    |  0
 .../download}/main.nf                         |  0
 .../download}/meta.yml                        |  4 +-
 nextflow.config                               |  7 +-
 nextflow_schema.json                          | 63 +++++++++++++++
 subworkflows/local/prepare_genome.nf          | 38 ++++++---
 workflows/rnafusion.nf                        | 25 ++++--
 24 files changed, 234 insertions(+), 196 deletions(-)
 rename modules/local/fusioncatcher/{ => detection}/functions.nf (100%)
 rename modules/local/fusioncatcher/{ => detection}/main.nf (63%)
 rename modules/local/fusioncatcher/{ => detection}/meta.yml (100%)
 rename modules/local/{fusioncatcher_downloadgenome => fusioncatcher/download}/functions.nf (100%)
 create mode 100644 modules/local/fusioncatcher/download/main.nf
 create mode 100644 modules/local/fusioncatcher/download/meta.yml
 delete mode 100644 modules/local/fusioncatcher_downloadgenome/main.nf
 delete mode 100644 modules/local/fusioncatcher_downloadgenome/meta.yml
 rename modules/local/starfusion/{ => detection}/functions.nf (100%)
 rename modules/local/starfusion/{ => detection}/main.nf (54%)
 rename modules/local/starfusion/{ => detection}/meta.yml (100%)
 rename modules/local/{starfusion_downloadgenome => starfusion/download}/functions.nf (100%)
 rename modules/local/{starfusion_downloadgenome => starfusion/download}/main.nf (100%)
 rename modules/local/{starfusion_downloadgenome => starfusion/download}/meta.yml (92%)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index ed90a293..ca680d0a 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -15,10 +15,7 @@ jobs:
     steps:
       - name: Launch workflow via tower
         uses: nf-core/tower-action@master
-        # TODO nf-core: You can customise AWS full pipeline tests as required
-        # Add full size test data (but still relatively small datasets for few samples)
-        # on the `test_full.config` test runs with only one set of parameters
-        
+
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }}
diff --git a/conf/modules.config b/conf/modules.config
index df3ed1f1..032d9a02 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -23,18 +23,22 @@
 params {
     modules {
         'fastqc' {
-            args = "--quiet"
+            args            = "--quiet"
         }
         'multiqc' {
-            args = ""
+            args            = ""
         }
        'star_genomegenerate' {
             args            = ""
             publish_dir     = 'genome/index'
         }
-        'starfusion_genomegenerate' {
-            args            = "--strip-components=2"
-            publish_dir     = 'genome/starfusion_resource'
+        'starfusion_download' {
+            args            = ""
+            publish_dir     = 'genome/resources'
+        }
+        'fusioncatcher_download' {
+            args            = ""
+            publish_dir     = 'genome/resources'
         }
         'star_align' {
             args            = "--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
@@ -51,5 +55,10 @@ params {
             publish_dir     = 'fusions/starfusion'
             publish_files   = ['tsv':'']
         }
+        'fusioncatcher' {
+            args            = ""
+            publish_dir     = 'fusions/fusioncatcher'
+            publish_files   = ['tsv':'']
+        }
     }
 }
diff --git a/conf/test.config b/conf/test.config
index 4808bd5f..663dcbcd 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -30,5 +30,4 @@ params {
     //STAR index
     star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
 
-    //STAR-Fusion
-    starfusion_genome   = 'https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.10/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz'
+}
diff --git a/docs/output.md b/docs/output.md
index 8a3fc362..0a6ec2f2 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -6,8 +6,6 @@ This document describes the output produced by the pipeline. Most of the plots a
 
 The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory.
 
-<!-- TODO nf-core: Write this documentation describing your workflow's output -->
-
 ## Pipeline overview
 
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
diff --git a/docs/usage.md b/docs/usage.md
index 7f14f65b..d94f8d6e 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -6,7 +6,9 @@
 
 ## Introduction
 
-<!-- TODO nf-core: Add documentation about anything specific to running your pipeline. For general topics, please point to (and add to) the main nf-core website. -->
+RNA sequencing analysis pipeline with curated list of tools for detecting and visualizing fusion genes.
+
+The pipeline is built using Nextflow, a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The Nextflow DSL2 implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from nf-core/modules in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
 
 ## Samplesheet input
 
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index 4caed266..cc5f9ad1 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -9,7 +9,6 @@ class WorkflowMain {
     //
     public static String citation(workflow) {
         return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
-            // TODO nf-core: Add Zenodo DOI for pipeline after first release
             //"* The pipeline\n" +
             //"  https://doi.org/10.5281/zenodo.XXXXXXX\n\n" +
             "* The nf-core framework\n" +
diff --git a/modules/local/fusioncatcher/functions.nf b/modules/local/fusioncatcher/detection/functions.nf
similarity index 100%
rename from modules/local/fusioncatcher/functions.nf
rename to modules/local/fusioncatcher/detection/functions.nf
diff --git a/modules/local/fusioncatcher/main.nf b/modules/local/fusioncatcher/detection/main.nf
similarity index 63%
rename from modules/local/fusioncatcher/main.nf
rename to modules/local/fusioncatcher/detection/main.nf
index 0cd4049b..1f2a4405 100644
--- a/modules/local/fusioncatcher/main.nf
+++ b/modules/local/fusioncatcher/detection/main.nf
@@ -13,9 +13,9 @@ process FUSIONCATCHER {
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_0"
+        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_1"
     } else {
-        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_0"
+        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_1"
     }
 
     input:
@@ -32,31 +32,20 @@ process FUSIONCATCHER {
     def software = getSoftwareName(task.process)
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     def fastq    = meta.single_end ? "${reads[0]}" : "${reads[0]},${reads[1]}"
-    def valid    = resource.exists() && file("${resource}/organism.txt").exists()
 
-    if (valid) {
+    """
+    fusioncatcher.py \\
+        -d $resource \\
+        -i $fastq \\
+        -p $task.cpus \\
+        -o . \\
+        $options.args
 
-        """
-        fusioncatcher \\
-            -d $resource \\
-            -i $fastq \\
-            -p $task.cpus \\
-            -o . \\
-            $options.args
+    mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher-fusion-genes.txt
+    mv summary_candidate_fusions.txt ${prefix}.fusioncatcher_summary.txt
+    mv fusioncatcher.log ${prefix}.fusioncatcher.log
 
-        mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher-fusion-genes.txt
-        mv summary_candidate_fusions.txt ${prefix}.fusioncatcher_summary.txt
-        mv fusioncatcher.log ${prefix}.fusioncatcher.log
+    fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
 
-        fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
-        """
-
-    }
-    else{
-
-        """
-        echo "Error: Invalid genome database" > ${prefix}.fusioncatcher.log
-        fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
-        """
-    }
+    """
 }
diff --git a/modules/local/fusioncatcher/meta.yml b/modules/local/fusioncatcher/detection/meta.yml
similarity index 100%
rename from modules/local/fusioncatcher/meta.yml
rename to modules/local/fusioncatcher/detection/meta.yml
diff --git a/modules/local/fusioncatcher_downloadgenome/functions.nf b/modules/local/fusioncatcher/download/functions.nf
similarity index 100%
rename from modules/local/fusioncatcher_downloadgenome/functions.nf
rename to modules/local/fusioncatcher/download/functions.nf
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
new file mode 100644
index 00000000..5ff2d638
--- /dev/null
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -0,0 +1,39 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process FUSIONCATCHER_DOWNLOADGENOME {
+    tag '$organism'
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'resources', meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_1"
+    } else {
+        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_1"
+    }
+
+    input:
+    val organism
+
+    output:
+    path "fusioncatcher-genome"     , emit: reference
+    path "*.version.txt"            , emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+
+    """
+    fusioncatcher-build \\
+        -g $organism \\
+        -o fusioncatcher-genome \\
+        $options.args
+
+    fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
+    """
+}
diff --git a/modules/local/fusioncatcher/download/meta.yml b/modules/local/fusioncatcher/download/meta.yml
new file mode 100644
index 00000000..419c6db5
--- /dev/null
+++ b/modules/local/fusioncatcher/download/meta.yml
@@ -0,0 +1,31 @@
+name: fusioncatcher_download
+description: Build genome for fusioncatcher
+keywords:
+  - sort
+tools:
+  - fusioncatcher:
+      description: Build genome for fusioncatcher
+      homepage: https://github.com/ndaniel/fusioncatcher/
+      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
+      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
+      doi: "10.1101/011650"
+      licence: ['GPL v3']
+
+input:
+  - organism:
+      type: value
+      description: Organism for which the data is downloaded from Ensembl database and built
+      pattern: "*"
+
+output:
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+  - resources:
+      type: directory
+      description: directory containing the genome resource files required for fusioncatcher
+      pattern: "fusioncatcher-genome"
+
+authors:
+  - "@praveenraj2018"
diff --git a/modules/local/fusioncatcher_downloadgenome/main.nf b/modules/local/fusioncatcher_downloadgenome/main.nf
deleted file mode 100644
index a71b4709..00000000
--- a/modules/local/fusioncatcher_downloadgenome/main.nf
+++ /dev/null
@@ -1,77 +0,0 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-// TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :)
-//               https://github.com/nf-core/modules/tree/master/software
-//               You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace:
-//               https://nf-co.re/join
-
-// TODO nf-core: A module file SHOULD only define input and output files as command-line parameters.
-//               All other parameters MUST be provided as a string i.e. "options.args"
-//               where "params.options" is a Groovy Map that MUST be provided via the addParams section of the including workflow.
-//               Any parameters that need to be evaluated in the context of a particular sample
-//               e.g. single-end/paired-end data MUST also be defined and evaluated appropriately.
-// TODO nf-core: Software that can be piped together SHOULD be added to separate module files
-//               unless there is a run-time, storage advantage in implementing in this way
-//               e.g. it's ok to have a single module for bwa to output BAM instead of SAM:
-//                 bwa mem | samtools view -B -T ref.fasta
-// TODO nf-core: Optional inputs are not currently supported by Nextflow. However, using an empty
-//               list (`[]`) instead of a file can be used to work around this issue.
-
-params.options = [:]
-options        = initOptions(params.options)
-
-process FUSIONCATCHER_DOWNLOADGENOME {
-    tag '$bam'
-    label 'process_low'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
-
-    // TODO nf-core: List required Conda package(s).
-    //               Software MUST be pinned to channel (i.e. "bioconda"), version (i.e. "1.10").
-    //               For Conda, the build (i.e. "h9402c20_2") must be EXCLUDED to support installation on different operating systems.
-    // TODO nf-core: See section in main README for further information regarding finding and adding container addresses to the section below.
-    conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_0"
-    } else {
-        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_0"
-    }
-
-    input:
-    // TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group"
-    //               MUST be provided as an input via a Groovy Map called "meta".
-    //               This information may not be required in some instances e.g. indexing reference genome files:
-    //               https://github.com/nf-core/modules/blob/master/software/bwa/index/main.nf
-    // TODO nf-core: Where applicable please provide/convert compressed files as input/output
-    //               e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc.
-    path bam
-
-    output:
-    // TODO nf-core: Named file extensions MUST be emitted for ALL output channels
-    path "*.bam", emit: bam
-    // TODO nf-core: List additional required output channels/values here
-    path "*.version.txt"          , emit: version
-
-    script:
-    def software = getSoftwareName(task.process)
-    
-    // TODO nf-core: Where possible, a command MUST be provided to obtain the version number of the software e.g. 1.10
-    //               If the software is unable to output a version number on the command-line then it can be manually specified
-    //               e.g. https://github.com/nf-core/modules/blob/master/software/homer/annotatepeaks/main.nf
-    // TODO nf-core: It MUST be possible to pass additional parameters to the tool as a command-line string via the "$options.args" variable
-    // TODO nf-core: If the tool supports multi-threading then you MUST provide the appropriate parameter
-    //               using the Nextflow "task" variable e.g. "--threads $task.cpus"
-    // TODO nf-core: Please replace the example samtools command below with your module's command
-    // TODO nf-core: Please indent the command appropriately (4 spaces!!) to help with readability ;)
-    """
-    samtools \\
-        sort \\
-        $options.args \\
-        -@ $task.cpus \\
-        $bam
-
-    echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt
-    """
-}
diff --git a/modules/local/fusioncatcher_downloadgenome/meta.yml b/modules/local/fusioncatcher_downloadgenome/meta.yml
deleted file mode 100644
index 050e987b..00000000
--- a/modules/local/fusioncatcher_downloadgenome/meta.yml
+++ /dev/null
@@ -1,37 +0,0 @@
-name: fusioncatcher_downloadgenome
-## TODO nf-core: Add a description of the module and list keywords
-description: write your description here
-keywords:
-  - sort
-tools:
-  - fusioncatcher:
-      ## TODO nf-core: Add a description and other details for the software below
-      description: Finder of Somatic Fusion Genes in RNA-seq data.
-      homepage: None
-      documentation: None
-      tool_dev_url: None
-      doi: ""
-      licence: ['GPL v3']
-
-## TODO nf-core: Add a description of all of the variables used as input
-input:
-  ## TODO nf-core: Delete / customise this example input
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-
-## TODO nf-core: Add a description of all of the variables used as output
-output:
-  - version:
-      type: file
-      description: File containing software version
-      pattern: "*.{version.txt}"
-  ## TODO nf-core: Delete / customise this example output
-  - bam:
-      type: file
-      description: Sorted BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-
-authors:
-  - "@praveenraj2018"
diff --git a/modules/local/starfusion/functions.nf b/modules/local/starfusion/detection/functions.nf
similarity index 100%
rename from modules/local/starfusion/functions.nf
rename to modules/local/starfusion/detection/functions.nf
diff --git a/modules/local/starfusion/main.nf b/modules/local/starfusion/detection/main.nf
similarity index 54%
rename from modules/local/starfusion/main.nf
rename to modules/local/starfusion/detection/main.nf
index b55de370..74317961 100644
--- a/modules/local/starfusion/main.nf
+++ b/modules/local/starfusion/detection/main.nf
@@ -27,27 +27,17 @@ process STARFUSION {
     path "*.version.txt"                                , emit: version
 
     script:
-    def software            = getSoftwareName(task.process)
-    def prefix              = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def fastq               = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
-    def genome_resource     = true //genome_resource_lib.exists() && file("${genome_resource_lib}/AnnotFilterRule.pm").exists()
-
-    if (genome_resource) {
-
-        """
-        STAR-Fusion --genome_lib_dir $genome_resource_lib \\
-            $fastq \\
-            --output_dir . \\
-            $options.args
-
-        echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > ${software}.version.txt
-        """
-    }
-    else{
-
-        """
-        echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > ${software}.version.txt
-        """
-
-    }
+    def software    = getSoftwareName(task.process)
+    def prefix      = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def fastq       = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
+
+    """
+    STAR-Fusion \\
+        --genome_lib_dir $genome_resource_lib \\
+        $fastq \\
+        --output_dir . \\
+        $options.args
+
+    echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > ${software}.version.txt
+    """
 }
diff --git a/modules/local/starfusion/meta.yml b/modules/local/starfusion/detection/meta.yml
similarity index 100%
rename from modules/local/starfusion/meta.yml
rename to modules/local/starfusion/detection/meta.yml
diff --git a/modules/local/starfusion_downloadgenome/functions.nf b/modules/local/starfusion/download/functions.nf
similarity index 100%
rename from modules/local/starfusion_downloadgenome/functions.nf
rename to modules/local/starfusion/download/functions.nf
diff --git a/modules/local/starfusion_downloadgenome/main.nf b/modules/local/starfusion/download/main.nf
similarity index 100%
rename from modules/local/starfusion_downloadgenome/main.nf
rename to modules/local/starfusion/download/main.nf
diff --git a/modules/local/starfusion_downloadgenome/meta.yml b/modules/local/starfusion/download/meta.yml
similarity index 92%
rename from modules/local/starfusion_downloadgenome/meta.yml
rename to modules/local/starfusion/download/meta.yml
index 91e3ecdc..ddcf69ee 100644
--- a/modules/local/starfusion_downloadgenome/meta.yml
+++ b/modules/local/starfusion/download/meta.yml
@@ -19,9 +19,9 @@ input:
 
 output:
   - reference:
-      type: file
+      type: directory
       description: Genome resource path
-      pattern: "*"
+      pattern: "star-fusion-genome"
 
 authors:
   - "@praveenraj2018"
diff --git a/nextflow.config b/nextflow.config
index c19ce907..67f5d55d 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -28,6 +28,7 @@ params {
     star_ignore_sjdbgtf        = false
     seq_center                 = null
     seq_platform               = null
+    star_index                 = null
 
     //Enable or disable tools
     arriba                     = true
@@ -37,6 +38,11 @@ params {
     squid                      = false
     pizzly                     = false
 
+    //genome resources
+    starfusion_genome          = null
+    fusioncatcher_genome       = null
+
+
     // Boilerplate options
     outdir                     = './results'
     tracedir                   = "${params.outdir}/pipeline_info"
@@ -66,7 +72,6 @@ params {
     max_memory                 = '128.GB'
     max_cpus                   = 16
     max_time                   = '240.h'
-
 }
 
 // Load base.config by default for all pipelines
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 9b68bb47..7ae5077d 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -302,6 +302,69 @@
                     "hidden": true,
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "Setting false will use GTF file for STAR alignment"
+                },
+                "star_index": {
+                    "type": "string",
+                    "description": "Path of STAR index directory",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Provide the index directory for the STAR alignment"
+                },
+                "starfusion_genome": {
+                    "type": "string",
+                    "description": "Path of the genome resource directory of STAR-Fusion",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Provide the genome resource data for the STAR-Fusion"
+                },
+                "fusioncatcher_genome": {
+                    "type": "string",
+                    "description": "Path of the genome resource directory of FusionCatcher",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Provide the path of the resource data path for FusionCatcher"
+                },
+                "arriba": {
+                    "type": "boolean",
+                    "description": "List of read aligners to be used",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Specify whether to run arriba or not"
+                },
+                "starfusion": {
+                    "type": "boolean",
+                    "description": "List of read aligners to be used",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Specify whether to run starfusion or not"
+                },
+                "fusioncatcher": {
+                    "type": "boolean",
+                    "description": "List of read aligners to be used",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Specify whether to run fusioncatcher or not"
+                },
+                "ericscript": {
+                    "type": "boolean",
+                    "description": "List of read aligners to be used",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Specify whether to run ericscript or not"
+                },
+                "squid": {
+                    "type": "boolean",
+                    "description": "List of read aligners to be used",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Specify whether to run squid or not"
+                },
+                "pizzly": {
+                    "type": "boolean",
+                    "description": "List of read aligners to be used",
+                    "hidden": true,
+                    "fa_icon": "fas fa-toolbox",
+                    "help_text": "Specify whether to run pizzly or not"
                 }
             }
         }
diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf
index 85d4c775..945eae68 100644
--- a/subworkflows/local/prepare_genome.nf
+++ b/subworkflows/local/prepare_genome.nf
@@ -2,15 +2,19 @@
 // Uncompress and prepare reference genome files
 //
 
-params.genome_options               = [:]
-params.star_index_options           = [:]
-params.starfusion_genome_options    = [:]
+params.genome_options                   = [:]
+params.star_index_options               = [:]
+params.starfusion_untar_options         = [:]
+params.starfusion_download_options      = [:]
+params.fusioncatcher_download_options   = [:]
 
 include { GUNZIP as GUNZIP_FASTA                } from '../../modules/nf-core/modules/gunzip/main'                  addParams( options: params.genome_options )
 include { UNTAR as UNTAR_STAR_INDEX             } from '../../modules/nf-core/modules/untar/main'                   addParams( options: params.star_index_options )
-include { UNTAR as UNTAR_STARFUSION_GENOME      } from '../../modules/nf-core/modules/untar/main'                   addParams( options: params.starfusion_genome_options )
+include { UNTAR as UNTAR_STARFUSION_GENOME      } from '../../modules/nf-core/modules/untar/main'                   addParams( options: params.starfusion_untar_options )
 include { SAMTOOLS_FAIDX                        } from '../../modules/nf-core/modules/samtools/faidx/main'          addParams( options: params.genome_options )
 include { STAR_GENOMEGENERATE                   } from '../../modules/nf-core/modules/star/genomegenerate/main'     addParams( options: params.star_index_options )
+include { STARFUSION_DOWNLOADGENOME             } from '../../modules/local/starfusion/download/main'               addParams( options: params.starfusion_download_options)
+include { FUSIONCATCHER_DOWNLOADGENOME          } from '../../modules/local/fusioncatcher/download/main'            addParams( options: params.fusioncatcher_download_options)
 
 workflow PREPARE_GENOME {
 
@@ -59,7 +63,8 @@ workflow PREPARE_GENOME {
         if (params.starfusion_genome) {
             ch_sf_genome = params.starfusion_genome
             if (ch_sf_genome.endsWith('.tar.gz')){
-                ch_starfusion_resource = UNTAR_STARFUSION_GENOME (ch_sf_genome).untar
+                root_path = UNTAR_STARFUSION_GENOME (ch_sf_genome).untar
+                ch_starfusion_resource = file("${root_path}/ctat_genome_lib_build_dir")
             }
             else if(file("${ch_sf_genome}/AnnotFilterRule.pm").exists()){
                 ch_starfusion_resource = file(ch_sf_genome)
@@ -70,12 +75,25 @@ workflow PREPARE_GENOME {
         }
     }
 
+    //Check genome data required for FusionCatcher
+    ch_fusioncatcher_resource = Channel.empty()
+    if (params.fusioncatcher){
+        if (params.fusioncatcher_genome){
+            ch_fusioncatcher_resource = file(params.fusioncatcher_genome)
+        }
+        else{
+            ch_fusioncatcher_resource = FUSIONCATCHER_DOWNLOADGENOME (params.organism).out.reference
+        }
+    }
+
     emit:
-    fasta               = ch_fasta            // path: genome.fasta
-    fai                 = ch_fasta_fai        // path: genome.fasta.fai
-    gtf                 = ch_gtf              // path: genome.gtf
-    star_index          = ch_star_index       // path: star/index/
-    starfusion_resource = ch_starfusion_resource
+    fasta                   = ch_fasta                  // path: genome.fasta
+    fai                     = ch_fasta_fai              // path: genome.fasta.fai
+    gtf                     = ch_gtf                    // path: genome.gtf
+    star_index              = ch_star_index             // path: star/index/
+    starfusion_resource     = ch_starfusion_resource    // path: starfusion genome
+    fusioncatcher_resource  = ch_fusioncatcher_resource // path: fusioncatcher genome
+
 
 }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 15de3b41..17924c1b 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -44,7 +44,8 @@ def modules = params.modules.clone()
 def publish_genome_options = params.save_reference ? [publish_dir: 'genome']       : [publish_files: false]
 def publish_index_options  = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false]
 def star_genomegenerate_options = modules['star_genomegenerate']
-def starfusion_genome_options = modules['starfusion_genomegenerate']
+def starfusion_genome_options = modules['starfusion_download']
+def fusioncatcher_genome_options = modules['fusioncatcher_download']
 if (!params.save_reference)     { star_genomegenerate_options['publish_files'] = false }
 
 // MODULE: Local to the pipeline
@@ -54,7 +55,7 @@ include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions'
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
 include { INPUT_CHECK } from '../subworkflows/local/input_check'                addParams( options: [:] )
-include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome'          addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options, starfusion_genome_options: starfusion_genome_options)
+include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome'          addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options, starfusion_untar_options: starfusion_genome_options, starfusion_download_options: starfusion_genome_options, fusioncatcher_download_options: fusioncatcher_genome_options)
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
@@ -67,10 +68,11 @@ multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"
 //
 // MODULE: Installed directly from nf-core/modules
 //
-include { FASTQC  }             from '../modules/nf-core/modules/fastqc/main'           addParams( options: modules['fastqc'] )
-include { MULTIQC }             from '../modules/nf-core/modules/multiqc/main'          addParams( options: multiqc_options   )
-include { FUSION_STAR_ARRIBA }  from '../subworkflows/nf-core/fusion_star_arriba'       addParams( star_align_options: modules['star_align'], arriba_options: modules['arriba_fusion'])
-include { STARFUSION }          from '../modules/local/starfusion/main'                 addParams( options: modules['starfusion'] )
+include { FASTQC                }   from '../modules/nf-core/modules/fastqc/main'           addParams( options: modules['fastqc'] )
+include { MULTIQC               }   from '../modules/nf-core/modules/multiqc/main'          addParams( options: multiqc_options   )
+include { FUSION_STAR_ARRIBA    }   from '../subworkflows/nf-core/fusion_star_arriba'       addParams( star_align_options: modules['star_align'], arriba_options: modules['arriba_fusion'])
+include { STARFUSION            }   from '../modules/local/starfusion/detection/main'       addParams( options: modules['starfusion'] )
+include { FUSIONCATCHER         }   from '../modules/local/fusioncatcher/detection/main'    addParams( options: modules['fusioncatcher'] )
 
 /*
 ========================================================================================
@@ -159,6 +161,17 @@ workflow RNAFUSION {
             ch_reads,
             PREPARE_GENOME.out.starfusion_resource
         )
+        ch_starfusion_fusions = STARFUSION.out.fusions
+    }
+
+    //Run FusionCatcher
+    if (params.fusioncatcher){
+        ch_fusioncather_fusions = Channel.empty()
+        FUSIONCATCHER (
+            ch_reads,
+            PREPARE_GENOME.out.fusioncatcher_resource
+        )
+        ch_fusioncatcher_fusions = FUSIONCATCHER.out.fusions
     }
 }
 

From 709648cc88281d6d165af9c04593100bcb8c82af Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Wed, 11 Aug 2021 16:06:50 +0200
Subject: [PATCH 042/359] Fix: Lint errors

---
 CHANGELOG.md                                  |  5 +--
 conf/modules.config                           | 40 +++++++++----------
 modules/local/fusioncatcher/download/main.nf  |  4 +-
 modules/local/fusioncatcher/download/meta.yml |  2 +-
 modules/local/starfusion/download/main.nf     |  2 +-
 subworkflows/local/prepare_genome.nf          | 20 +++-------
 .../{fusion_star_arriba.nf => arriba.nf}      |  0
 workflows/rnafusion.nf                        |  2 +-
 8 files changed, 32 insertions(+), 43 deletions(-)
 rename subworkflows/nf-core/{fusion_star_arriba.nf => arriba.nf} (100%)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e4e481eb..9ced91bd 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,13 +3,12 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v1.0dev - [date]
+## v1.0dev - [8/11/2021]
 
 Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
 
 ### `Added`
-1. Added STAR Alignment followed by Arriba fusion calling
-2. Added STAR Fusion calling
+[#192](https://github.com/nf-core/rnafusion/pull/192) - Added STAR-fusion (genome download and fusion calling as local modules) into pipeline
 
 ### `Fixed`
 
diff --git a/conf/modules.config b/conf/modules.config
index 032d9a02..449f500b 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -23,42 +23,42 @@
 params {
     modules {
         'fastqc' {
-            args            = "--quiet"
+            args                = "--quiet"
         }
         'multiqc' {
-            args            = ""
+            args                = ""
         }
        'star_genomegenerate' {
-            args            = ""
-            publish_dir     = 'genome/index'
+            args                = ""
+            publish_dir         = 'genome/index'
         }
         'starfusion_download' {
-            args            = ""
-            publish_dir     = 'genome/resources'
+            args                = ""
+            publish_dir         = 'genome/resources'
         }
         'fusioncatcher_download' {
-            args            = ""
-            publish_dir     = 'genome/resources'
+            args                = ""
+            publish_dir         = 'genome/resources'
         }
         'star_align' {
-            args            = "--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
-            publish_dir     = "alignment/${params.aligner}"
-            publish_files   = ['bam':'', 'junction':'', 'out':'log', 'tab':'log']
+            args                = "--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
+            publish_dir         = "alignment/${params.aligner}"
+            publish_files       = ['bam':'', 'junction':'', 'out':'log', 'tab':'log']
         }
         'arriba_fusion' {
-            args            = ""
-            publish_dir     = 'fusions/arriba'
-            publish_files   = ['tsv':'']
+            args                = ""
+            publish_dir         = 'fusions/arriba'
+            publish_files       = ['tsv':'']
         }
         'starfusion' {
-            args            = "--examine_coding_effect"
-            publish_dir     = 'fusions/starfusion'
-            publish_files   = ['tsv':'']
+            args                = "--examine_coding_effect"
+            publish_dir         = 'fusions/starfusion'
+            publish_files       = ['tsv':'']
         }
         'fusioncatcher' {
-            args            = ""
-            publish_dir     = 'fusions/fusioncatcher'
-            publish_files   = ['tsv':'']
+            args                = ""
+            publish_dir         = 'fusions/fusioncatcher'
+            publish_files       = ['tsv':'']
         }
     }
 }
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 5ff2d638..466c055a 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -22,8 +22,8 @@ process FUSIONCATCHER_DOWNLOADGENOME {
     val organism
 
     output:
-    path "fusioncatcher-genome"     , emit: reference
-    path "*.version.txt"            , emit: version
+    path "fusioncatcher"    , emit: reference
+    path "*.version.txt"    , emit: version
 
     script:
     def software = getSoftwareName(task.process)
diff --git a/modules/local/fusioncatcher/download/meta.yml b/modules/local/fusioncatcher/download/meta.yml
index 419c6db5..1e1e7426 100644
--- a/modules/local/fusioncatcher/download/meta.yml
+++ b/modules/local/fusioncatcher/download/meta.yml
@@ -22,7 +22,7 @@ output:
       type: file
       description: File containing software version
       pattern: "*.{version.txt}"
-  - resources:
+  - reference:
       type: directory
       description: directory containing the genome resource files required for fusioncatcher
       pattern: "fusioncatcher-genome"
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 46fa3a17..2768f686 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -15,7 +15,7 @@ process STARFUSION_DOWNLOADGENOME {
     val genome_build
 
     output:
-    path "star-fusion-genome"   , emit: reference
+    path "star-fusion"  , emit: reference
 
     script:
     def software = getSoftwareName(task.process)
diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf
index 945eae68..52f628b2 100644
--- a/subworkflows/local/prepare_genome.nf
+++ b/subworkflows/local/prepare_genome.nf
@@ -24,21 +24,15 @@ workflow PREPARE_GENOME {
     main:
 
     // Uncompress genome fasta file if required
-    if (params.fasta.endsWith('.gz'))   ch_fasta = GUNZIP_FASTA ( params.fasta ).gunzipe
-    else                                ch_fasta = file(params.fasta)
+    ch_fasta = params.fasta.endsWith('.gz') ? GUNZIP_FASTA ( params.fasta ).gunzipe : file(params.fasta)
 
     // Index the genome fasta
     ch_fasta_fai = Channel.empty()
-    if (params.fasta_fai)               ch_fasta_fai = file(params.fasta_fai)
-    else                                ch_fasta_fai = SAMTOOLS_FAIDX(ch_fasta).fai
+    ch_fasta_fai = params.fasta_fai ? file(params.fasta_fai) : SAMTOOLS_FAIDX(ch_fasta).fai
 
     // Get the GTF file
     if (params.gtf) {
-        if (params.gtf.endsWith('.gz')) {
-            ch_gtf = GUNZIP_GTF ( params.gtf ).gunzip
-        } else {
-            ch_gtf = file(params.gtf)
-        }
+        ch_gtf = params.gtf.endsWith('.gz') ? GUNZIP_GTF ( params.gtf ).gunzip : file(params.gtf)
     }
 
     // Uncompress STAR index or generate from scratch if required
@@ -46,11 +40,7 @@ workflow PREPARE_GENOME {
     ch_star_version = Channel.empty()
     if ('star' in prepare_tool_indices) {
         if (params.star_index) {
-            if (params.star_index.endsWith('.tar.gz')) {
-                ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar
-            } else {
-                ch_star_index = file(params.star_index)
-            }
+            ch_star_index = params.star_index.endsWith('.tar.gz') ? UNTAR_STAR_INDEX ( params.star_index ).untar : file(params.star_index)
         } else {
             ch_star_index   = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index
             ch_star_version = STAR_GENOMEGENERATE.out.version
@@ -66,7 +56,7 @@ workflow PREPARE_GENOME {
                 root_path = UNTAR_STARFUSION_GENOME (ch_sf_genome).untar
                 ch_starfusion_resource = file("${root_path}/ctat_genome_lib_build_dir")
             }
-            else if(file("${ch_sf_genome}/AnnotFilterRule.pm").exists()){
+            else {
                 ch_starfusion_resource = file(ch_sf_genome)
             }
         }
diff --git a/subworkflows/nf-core/fusion_star_arriba.nf b/subworkflows/nf-core/arriba.nf
similarity index 100%
rename from subworkflows/nf-core/fusion_star_arriba.nf
rename to subworkflows/nf-core/arriba.nf
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 17924c1b..0d35afc7 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -70,7 +70,7 @@ multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"
 //
 include { FASTQC                }   from '../modules/nf-core/modules/fastqc/main'           addParams( options: modules['fastqc'] )
 include { MULTIQC               }   from '../modules/nf-core/modules/multiqc/main'          addParams( options: multiqc_options   )
-include { FUSION_STAR_ARRIBA    }   from '../subworkflows/nf-core/fusion_star_arriba'       addParams( star_align_options: modules['star_align'], arriba_options: modules['arriba_fusion'])
+include { FUSION_STAR_ARRIBA    }   from '../subworkflows/nf-core/arriba'       addParams( star_align_options: modules['star_align'], arriba_options: modules['arriba_fusion'])
 include { STARFUSION            }   from '../modules/local/starfusion/detection/main'       addParams( options: modules['starfusion'] )
 include { FUSIONCATCHER         }   from '../modules/local/fusioncatcher/detection/main'    addParams( options: modules['fusioncatcher'] )
 

From d80d2057747206eee47abd9c78f27e791109f964 Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Wed, 11 Aug 2021 16:13:25 +0200
Subject: [PATCH 043/359] Fix: Lint errors - attempt#2

---
 CHANGELOG.md        | 6 ++++--
 conf/modules.config | 2 +-
 2 files changed, 5 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9ced91bd..04ab5e42 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -1,14 +1,16 @@
 # nf-core/rnafusion: Changelog
 
+All notable changes to this project will be documented in this file.
+
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v1.0dev - [8/11/2021]
+## [dev](https://github.com/nf-core/rnafusion/tree/dsl2)
 
 Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
 
 ### `Added`
-[#192](https://github.com/nf-core/rnafusion/pull/192) - Added STAR-fusion (genome download and fusion calling as local modules) into pipeline
+-   [#192](https://github.com/nf-core/rnafusion/pull/192) - Added STAR-fusion (genome download and fusion calling as local modules) into pipeline
 
 ### `Fixed`
 
diff --git a/conf/modules.config b/conf/modules.config
index 449f500b..8af8a96d 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -28,7 +28,7 @@ params {
         'multiqc' {
             args                = ""
         }
-       'star_genomegenerate' {
+        'star_genomegenerate' {
             args                = ""
             publish_dir         = 'genome/index'
         }

From f30e0fea38b4936aa4fefd012f9c7c1d71b94a99 Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Wed, 11 Aug 2021 16:16:55 +0200
Subject: [PATCH 044/359] Fix: Lint errors - attempt#3

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 04ab5e42..e4f4c429 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -10,7 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
 
 ### `Added`
--   [#192](https://github.com/nf-core/rnafusion/pull/192) - Added STAR-fusion (genome download and fusion calling as local modules) into pipeline
+- [#192](https://github.com/nf-core/rnafusion/pull/192) - Added STAR-fusion (genome download and fusion calling as local modules) into pipeline
 
 ### `Fixed`
 

From 46648aad790550dd7c362be23d5c4edc2da9b721 Mon Sep 17 00:00:00 2001
From: Praveen <pravee1216@gmail.com>
Date: Wed, 11 Aug 2021 16:20:09 +0200
Subject: [PATCH 045/359] Fix: Lint errors - attempt#4

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e4f4c429..a87a2575 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
 
 ### `Added`
+
 - [#192](https://github.com/nf-core/rnafusion/pull/192) - Added STAR-fusion (genome download and fusion calling as local modules) into pipeline
 
 ### `Fixed`

From 296f48a90e8da06fb2c95d332cce21e6ce101137 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Wed, 11 Aug 2021 18:48:20 +0200
Subject: [PATCH 046/359] fix: added pipeline zenodo doi

---
 lib/WorkflowMain.groovy | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index cc5f9ad1..24c539d7 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -9,8 +9,8 @@ class WorkflowMain {
     //
     public static String citation(workflow) {
         return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
-            //"* The pipeline\n" +
-            //"  https://doi.org/10.5281/zenodo.XXXXXXX\n\n" +
+            "* The pipeline\n" +
+            "  https://doi.org/10.5281/zenodo.151721952\n\n" +
             "* The nf-core framework\n" +
             "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
             "* Software dependencies\n" +

From 1dc605301e8c7e43e8b87536879386771279d452 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Mon, 27 Sep 2021 10:59:21 +0200
Subject: [PATCH 047/359] wip: major refactor of pipeline

---
 conf/genomes.config                           |  25 +
 conf/igenomes.config                          | 432 ------------------
 conf/modules.config                           |  49 +-
 conf/test.config                              |  16 +-
 conf/test_full.config                         |  10 +-
 docs/usage.md                                 |   2 +-
 lib/WorkflowMain.groovy                       |   2 +-
 main.nf                                       |  23 +-
 modules.json                                  |  16 +-
 .../detection => arriba/detect}/functions.nf  |   0
 .../arriba => local/arriba/detect}/main.nf    |  21 +-
 .../download}/functions.nf                    |   0
 modules/local/arriba/download/main.nf         |  34 ++
 .../arriba => local/ensembl}/functions.nf     |   0
 modules/local/ensembl/main.nf                 |  44 ++
 .../ericscript/detect}/functions.nf           |   0
 modules/local/ericscript/detect/main.nf       |  44 ++
 .../ericscript/download}/functions.nf         |   0
 modules/local/ericscript/download/main.nf     |  35 ++
 .../fusioncatcher/detect}/functions.nf        |   0
 .../{detection => detect}/main.nf             |  17 +-
 .../{detection => detect}/meta.yml            |   0
 modules/local/fusioncatcher/download/main.nf  |  34 +-
 .../pizzly/detect}/functions.nf               |   0
 modules/local/pizzly/detect/main.nf           |  49 ++
 modules/local/pizzly/download/functions.nf    |  68 +++
 .../untar => local/pizzly/download}/main.nf   |  27 +-
 modules/local/star/align/functions.nf         |  68 +++
 .../modules => local}/star/align/main.nf      |   6 +-
 .../modules => local}/star/align/meta.yml     |   0
 .../local/star/genomegenerate/functions.nf    |  68 +++
 modules/local/star/genomegenerate/main.nf     |  47 ++
 .../star/genomegenerate/meta.yml              |   0
 modules/local/starfusion/detect/functions.nf  |  68 +++
 .../starfusion/{detection => detect}/main.nf  |  29 +-
 .../starfusion/{detection => detect}/meta.yml |   0
 modules/local/starfusion/download/main.nf     |  68 +--
 modules/local/starfusion/download/meta.yml    |   2 +-
 modules/nf-core/modules/arriba/meta.yml       |  54 ---
 modules/nf-core/modules/gunzip/meta.yml       |  27 --
 .../modules/kallisto/index/functions.nf       |  68 +++
 .../{gunzip => kallisto/index}/main.nf        |  26 +-
 .../nf-core/modules/kallisto/index/meta.yml   |  31 ++
 .../modules/star/genomegenerate/main.nf       |  67 ---
 modules/nf-core/modules/untar/meta.yml        |  27 --
 nextflow.config                               |  36 +-
 nextflow_schema.json                          |  91 ----
 workflows/build_references.nf                 |  68 +++
 workflows/rnafusion.nf                        |  13 -
 49 files changed, 920 insertions(+), 892 deletions(-)
 create mode 100644 conf/genomes.config
 delete mode 100644 conf/igenomes.config
 rename modules/local/{fusioncatcher/detection => arriba/detect}/functions.nf (100%)
 rename modules/{nf-core/modules/arriba => local/arriba/detect}/main.nf (80%)
 rename modules/local/{starfusion/detection => arriba/download}/functions.nf (100%)
 create mode 100644 modules/local/arriba/download/main.nf
 rename modules/{nf-core/modules/arriba => local/ensembl}/functions.nf (100%)
 create mode 100644 modules/local/ensembl/main.nf
 rename modules/{nf-core/modules/gunzip => local/ericscript/detect}/functions.nf (100%)
 create mode 100644 modules/local/ericscript/detect/main.nf
 rename modules/{nf-core/modules/star/align => local/ericscript/download}/functions.nf (100%)
 create mode 100644 modules/local/ericscript/download/main.nf
 rename modules/{nf-core/modules/star/genomegenerate => local/fusioncatcher/detect}/functions.nf (100%)
 rename modules/local/fusioncatcher/{detection => detect}/main.nf (81%)
 rename modules/local/fusioncatcher/{detection => detect}/meta.yml (100%)
 rename modules/{nf-core/modules/untar => local/pizzly/detect}/functions.nf (100%)
 create mode 100644 modules/local/pizzly/detect/main.nf
 create mode 100644 modules/local/pizzly/download/functions.nf
 rename modules/{nf-core/modules/untar => local/pizzly/download}/main.nf (54%)
 create mode 100644 modules/local/star/align/functions.nf
 rename modules/{nf-core/modules => local}/star/align/main.nf (95%)
 rename modules/{nf-core/modules => local}/star/align/meta.yml (100%)
 create mode 100644 modules/local/star/genomegenerate/functions.nf
 create mode 100644 modules/local/star/genomegenerate/main.nf
 rename modules/{nf-core/modules => local}/star/genomegenerate/meta.yml (100%)
 create mode 100644 modules/local/starfusion/detect/functions.nf
 rename modules/local/starfusion/{detection => detect}/main.nf (56%)
 rename modules/local/starfusion/{detection => detect}/meta.yml (100%)
 delete mode 100644 modules/nf-core/modules/arriba/meta.yml
 delete mode 100644 modules/nf-core/modules/gunzip/meta.yml
 create mode 100644 modules/nf-core/modules/kallisto/index/functions.nf
 rename modules/nf-core/modules/{gunzip => kallisto/index}/main.nf (54%)
 create mode 100644 modules/nf-core/modules/kallisto/index/meta.yml
 delete mode 100644 modules/nf-core/modules/star/genomegenerate/main.nf
 delete mode 100644 modules/nf-core/modules/untar/meta.yml
 create mode 100644 workflows/build_references.nf

diff --git a/conf/genomes.config b/conf/genomes.config
new file mode 100644
index 00000000..ec29a7c5
--- /dev/null
+++ b/conf/genomes.config
@@ -0,0 +1,25 @@
+/*
+ * -------------------------------------------------
+ *  Nextflow config file for custom genomes
+ * -------------------------------------------------
+ * These references have to be build manually due 
+ * to ERCC spike-ins.
+ * Can be used by any config that customizes the base
+ * path using $params.genomes_base / --genomes_base
+ */
+
+ params {
+  genomes {
+    'GRCh38' {
+      fasta         = "${params.genomes_base}/${params.genome}/Homo_sapiens.GRCh38.all.fa"
+      gtf           = "${params.genomes_base}/${params.genome}/Homo_sapiens.GRCh38.gtf"
+      transcript    = "${params.genomes_base}/${params.genome}/Homo_sapiens.GRCh38.cdna.all.fa.gz"
+      star          = "${params.genomes_base}/${params.genome}/STARIndex"
+      arriba        = "${params.genomes_base}/${params.genome}/arriba"
+      ericscript    = "${params.genomes_base}/${params.genome}/ericscript"
+      fusioncatcher = "${params.genomes_base}/${params.genome}/fusioncatcher"
+      pizzly        = "${params.genomes_base}/${params.genome}/pizzly"
+      starfusion    = "${params.genomes_base}/${params.genome}/starfusion"
+    }
+  }
+}
\ No newline at end of file
diff --git a/conf/igenomes.config b/conf/igenomes.config
deleted file mode 100644
index 855948de..00000000
--- a/conf/igenomes.config
+++ /dev/null
@@ -1,432 +0,0 @@
-/*
-========================================================================================
-    Nextflow config file for iGenomes paths
-========================================================================================
-    Defines reference genomes using iGenome paths.
-    Can be used by any config that customises the base path using:
-        $params.igenomes_base / --igenomes_base
-----------------------------------------------------------------------------------------
-*/
-
-params {
-    // illumina iGenomes reference file paths
-    genomes {
-        'GRCh37' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
-        }
-        'GRCh38' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-        }
-        'GRCm38' {
-            fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "1.87e9"
-            blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
-        }
-        'TAIR10' {
-            fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
-            mito_name   = "Mt"
-        }
-        'EB2' {
-            fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
-        }
-        'UMD3.1' {
-            fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'WBcel235' {
-            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
-            mito_name   = "MtDNA"
-            macs_gsize  = "9e7"
-        }
-        'CanFam3.1' {
-            fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'GRCz10' {
-            fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'BDGP6' {
-            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
-            mito_name   = "M"
-            macs_gsize  = "1.2e8"
-        }
-        'EquCab2' {
-            fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'EB1' {
-            fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
-        }
-        'Galgal4' {
-            fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'Gm01' {
-            fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
-        }
-        'Mmul_1' {
-            fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'IRGSP-1.0' {
-            fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
-            mito_name   = "Mt"
-        }
-        'CHIMP2.1.4' {
-            fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'Rnor_5.0' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'Rnor_6.0' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'R64-1-1' {
-            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-            macs_gsize  = "1.2e7"
-        }
-        'EF2' {
-            fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "1.21e7"
-        }
-        'Sbi1' {
-            fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
-        }
-        'Sscrofa10.2' {
-            fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'AGPv3' {
-            fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
-            mito_name   = "Mt"
-        }
-        'hg38' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-        }
-        'hg19' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
-        }
-        'mm10' {
-            fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "1.87e9"
-            blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
-        }
-        'bosTau8' {
-            fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-        }
-        'ce10' {
-            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "9e7"
-        }
-        'canFam3' {
-            fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'danRer10' {
-            fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "1.37e9"
-        }
-        'dm6' {
-            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "1.2e8"
-        }
-        'equCab2' {
-            fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'galGal4' {
-            fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'panTro4' {
-            fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'rn6' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-        }
-        'sacCer3' {
-            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
-            readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "1.2e7"
-        }
-        'susScr3' {
-            fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-    }
-}
diff --git a/conf/modules.config b/conf/modules.config
index 8af8a96d..fd8cf9da 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -28,37 +28,50 @@ params {
         'multiqc' {
             args                = ""
         }
-        'star_genomegenerate' {
+        'arriba' {
+            args                = "--outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
+            args2               = "-b references/${params.genome}/arriba/blacklist_hg38_GRCh38_v2.1.0.tsv.gz -k references/${params.genome}/arriba/known_fusions_hg38_GRCh38_v2.1.0.tsv.gz -p references/${params.genome}/arriba/protein_domains_hg38_GRCh38_v2.1.0.gff3"
+        }
+        'arriba_download' {
             args                = ""
-            publish_dir         = 'genome/index'
+            publish_dir         = "references/${params.genome}/arriba"
         }
-        'starfusion_download' {
+        'ensembl_download' {
+            args                = ""
+            publish_dir         = "references/${params.genome}"
+        }
+        'ericscript' {
+            args                = ""
+        }
+        'ericscript_download' {
+            args                = ""
+            publish_dir         = "references/${params.genome}/ericscript"
+        }
+        'fusioncatcher' {
             args                = ""
-            publish_dir         = 'genome/resources'
         }
         'fusioncatcher_download' {
             args                = ""
-            publish_dir         = 'genome/resources'
+            publish_dir         = "references/${params.genome}/fusioncatcher"
         }
-        'star_align' {
-            args                = "--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
-            publish_dir         = "alignment/${params.aligner}"
-            publish_files       = ['bam':'', 'junction':'', 'out':'log', 'tab':'log']
+        'pizzly' {
+            args                = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
         }
-        'arriba_fusion' {
+        'pizzly_download' {
+            args                = "-k 31"
+            publish_dir         = "references/${params.genome}/pizzly"
+        }
+        'star_index' {
             args                = ""
-            publish_dir         = 'fusions/arriba'
-            publish_files       = ['tsv':'']
+            publish_dir         = "references/${params.genome}/STARIndex"
         }
         'starfusion' {
-            args                = "--examine_coding_effect"
-            publish_dir         = 'fusions/starfusion'
-            publish_files       = ['tsv':'']
+            args                = "--chimSegmentMin 12 --chimJunctionOverhangMin 8 --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignIntronMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --outSAMattrRGline ID:GRPundef --chimMultimapScoreRange 3 --chimScoreJunctionNonGTAG -4 --chimMultimapNmax 20 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLminOverLmate 0 --alignSplicedMateMapLmin 30"
+            args2               = "--examine_coding_effect -J Chimeric.out.junction"
         }
-        'fusioncatcher' {
+        'starfusion_download' {
             args                = ""
-            publish_dir         = 'fusions/fusioncatcher'
-            publish_files       = ['tsv':'']
+            publish_dir         = "references/${params.genome}/starfusion"
         }
     }
 }
diff --git a/conf/test.config b/conf/test.config
index 663dcbcd..d1efcef0 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -15,19 +15,19 @@ params {
     config_profile_description = 'Minimal test dataset to check pipeline function'
 
     // Limit resources so that this can run on GitHub Actions
-    max_cpus   = 2
-    max_memory = 6.GB
-    max_time   = 6.h
+    // max_cpus   = 2
+    // max_memory = 6.GB
+    // max_time   = 6.h
 
     // Input data
-    input = 'assets/samplesheet_valid.csv'
+    // input = 'assets/samplesheet_valid.csv'
 
     // Genome references
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
-    fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
-    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf'
+    // fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
+    // fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
+    // gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf'
 
     //STAR index
-    star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
+    // star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
 
 }
diff --git a/conf/test_full.config b/conf/test_full.config
index 31e5e9c2..4304f216 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -15,13 +15,13 @@ params {
     config_profile_description = 'Full test dataset to check pipeline function'
 
     // Input data
-    input = 'assets/samplesheet_valid.csv'
+    // input = 'assets/samplesheet_valid.csv'
 
     // Genome references
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
-    fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
-    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf'
+    // fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
+    // fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
+    // gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf'
 
     //STAR index
-    star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
+    // star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
 }
diff --git a/docs/usage.md b/docs/usage.md
index d94f8d6e..f3dbed32 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -59,7 +59,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p
 The typical command for running the pipeline is as follows:
 
 ```console
-nextflow run nf-core/rnafusion --input samplesheet.csv --genome GRCh37 -profile docker
+nextflow run nf-core/rnafusion --input samplesheet.csv --genome GRCh38 -profile docker
 ```
 
 This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index 24c539d7..e15f4532 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -21,7 +21,7 @@ class WorkflowMain {
     // Print help to screen if required
     //
     public static String help(workflow, params, log) {
-        def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker"
+        def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh38 -profile docker"
         def help_string = ''
         help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs)
         help_string += NfcoreSchema.paramsHelp(workflow, params, command)
diff --git a/main.nf b/main.nf
index 2b33ffe8..c13e7b80 100644
--- a/main.nf
+++ b/main.nf
@@ -11,14 +11,6 @@
 
 nextflow.enable.dsl = 2
 
-/*
-========================================================================================
-    GENOME PARAMETER VALUES
-========================================================================================
-*/
-
-params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
-
 /*
 ========================================================================================
     VALIDATE & PRINT PARAMETER SUMMARY
@@ -33,13 +25,24 @@ WorkflowMain.initialise(workflow, params, log)
 ========================================================================================
 */
 
-include { RNAFUSION } from './workflows/rnafusion'
 
 //
 // WORKFLOW: Run main nf-core/rnafusion analysis pipeline
 //
 workflow NFCORE_RNAFUSION {
-    RNAFUSION ()
+
+    if (params.build_references) {
+
+        include { BUILD_REFERENCES } from './workflows/build_references'
+        BUILD_REFERENCES ()
+    
+    } else {
+
+        include { RNAFUSION } from './workflows/rnafusion'
+        // RNAFUSION ()
+
+    }
+
 }
 
 /*
diff --git a/modules.json b/modules.json
index 802ee198..7297e3d2 100644
--- a/modules.json
+++ b/modules.json
@@ -3,13 +3,10 @@
     "homePage": "https://github.com/nf-core/rnafusion",
     "repos": {
         "nf-core/modules": {
-            "arriba": {
-                "git_sha": "3cabc95d0ed8a5a4e07b8f9b1d1f7ff9a70f61e1"
-            },
             "fastqc": {
                 "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
             },
-            "gunzip": {
+            "kallisto/index": {
                 "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
             },
             "multiqc": {
@@ -17,16 +14,7 @@
             },
             "samtools/faidx": {
                 "git_sha": "c5235a983d454787fa0c3247b02086969217163b"
-            },
-            "star/align": {
-                "git_sha": "5de3f2c50e063bda30316342b6606b485a12e38c"
-            },
-            "star/genomegenerate": {
-                "git_sha": "f43778b0e641a9643ae73fe1f2fe2d5bb81edffc"
-            },
-            "untar": {
-                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/local/fusioncatcher/detection/functions.nf b/modules/local/arriba/detect/functions.nf
similarity index 100%
rename from modules/local/fusioncatcher/detection/functions.nf
rename to modules/local/arriba/detect/functions.nf
diff --git a/modules/nf-core/modules/arriba/main.nf b/modules/local/arriba/detect/main.nf
similarity index 80%
rename from modules/nf-core/modules/arriba/main.nf
rename to modules/local/arriba/detect/main.nf
index 739922ef..e9707d87 100644
--- a/modules/nf-core/modules/arriba/main.nf
+++ b/modules/local/arriba/detect/main.nf
@@ -19,9 +19,10 @@ process ARRIBA {
     }
 
     input:
-    tuple val(meta), path(bam)
+    tuple val(meta), path(reads)
     path fasta
     path gtf
+    path index
 
     output:
     tuple val(meta), path("*.fusions.tsv")          , emit: fusions
@@ -31,16 +32,22 @@ process ARRIBA {
     script:
     def software  = getSoftwareName(task.process)
     def prefix    = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def blacklist = (options.args.contains('-b')) ? '' : '-f blacklist'
     """
+    STAR \\
+	    --genomeDir $index \\
+	    --readFilesIn $reads \\
+	    --runThreadN $task.cpus \\
+        $options.args |
+
+    tee Aligned.out.bam |
+
     arriba \\
-        -x $bam \\
+        -x /dev/stdin \\
         -a $fasta \\
         -g $gtf \\
-        -o ${prefix}.fusions.tsv \\
-        -O ${prefix}.fusions.discarded.tsv \\
-        $blacklist \\
-        $options.args
+        -o ${prefix}.arriba.fusions.tsv \\
+        -O ${prefix}.arriba.fusions.discarded.tsv \\
+        $options.args2
 
     echo \$(arriba -h | grep 'Version:' 2>&1) |  sed 's/Version:\s//' > ${software}.version.txt
     """
diff --git a/modules/local/starfusion/detection/functions.nf b/modules/local/arriba/download/functions.nf
similarity index 100%
rename from modules/local/starfusion/detection/functions.nf
rename to modules/local/arriba/download/functions.nf
diff --git a/modules/local/arriba/download/main.nf b/modules/local/arriba/download/main.nf
new file mode 100644
index 00000000..7cb029d2
--- /dev/null
+++ b/modules/local/arriba/download/main.nf
@@ -0,0 +1,34 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process ARRIBA_DOWNLOAD {
+    tag "arriba"
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5"
+    } else {
+        container "quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5"
+    }
+
+    output:
+    path "*.version.txt"           , emit: version
+    path "arriba_v2.1.0/database/*", emit: reference
+
+    script:
+    def software = getSoftwareName(task.process)
+    """
+    wget https://github.com/suhrig/arriba/releases/download/v2.1.0/arriba_v2.1.0.tar.gz -O arriba_v2.1.0.tar.gz
+    tar -xzvf arriba_v2.1.0.tar.gz
+    rm arriba_v2.1.0.tar.gz
+
+    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
+    """
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/arriba/functions.nf b/modules/local/ensembl/functions.nf
similarity index 100%
rename from modules/nf-core/modules/arriba/functions.nf
rename to modules/local/ensembl/functions.nf
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
new file mode 100644
index 00000000..42f9a33c
--- /dev/null
+++ b/modules/local/ensembl/main.nf
@@ -0,0 +1,44 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process ENSEMBL_DOWNLOAD {
+    tag "ensembl $ensembl_version"
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5"
+    } else {
+        container "quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5"
+    }
+
+    input:
+    val ensembl_version
+
+    output:
+    path "*.version.txt"                                                  , emit: version
+    path "Homo_sapiens.${params.genome}.${ensembl_version}.all.fa"        , emit: fasta
+    path "Homo_sapiens.${params.genome}.${ensembl_version}.gtf"           , emit: gtf
+    path "Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz", emit: transcript
+
+    script:
+    def software = getSoftwareName(task.process)
+    """
+    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
+    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
+
+    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
+    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz
+
+    gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa
+    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
+    
+    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
+    """
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/gunzip/functions.nf b/modules/local/ericscript/detect/functions.nf
similarity index 100%
rename from modules/nf-core/modules/gunzip/functions.nf
rename to modules/local/ericscript/detect/functions.nf
diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
new file mode 100644
index 00000000..94f6476d
--- /dev/null
+++ b/modules/local/ericscript/detect/main.nf
@@ -0,0 +1,44 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process ERICSCRIPT {
+    tag "eriscript"
+    label 'process_medium'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "bioconda::ericscript=0.5.5" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/ericscript:0.5.5--hdfd78af_5"
+    } else {
+        container "quay.io/biocontainers/ericscript:0.5.5--hdfd78af_5"
+    }
+
+    input:
+    tuple val(meta), path(reads)
+    path reference
+
+    output:
+    path "*.version.txt"                                                   , emit: version
+    tuple val(meta), path "./tmp/${prefix}.ericscript.results.filtered.tsv", emit: fusions
+    tuple val(meta), path "./tmp/${prefix}.ericscript.results.total.tsv"   , emit: fusions_total
+    
+    script:
+    def software = getSoftwareName(task.process)
+    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    """
+    ericscript.pl \\
+        -db $reference \\
+        -name ${prefix}.ericscript \\
+        -p $task.cpus \\
+        -o ./tmp \\
+        $reads \\
+        $options.args
+
+    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
+    """
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/star/align/functions.nf b/modules/local/ericscript/download/functions.nf
similarity index 100%
rename from modules/nf-core/modules/star/align/functions.nf
rename to modules/local/ericscript/download/functions.nf
diff --git a/modules/local/ericscript/download/main.nf b/modules/local/ericscript/download/main.nf
new file mode 100644
index 00000000..86307736
--- /dev/null
+++ b/modules/local/ericscript/download/main.nf
@@ -0,0 +1,35 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process ERICSCRIPT_DOWNLOAD {
+    tag "eriscript"
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "bioconda::ericscript=0.5.5" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/ericscript:0.5.5--hdfd78af_5"
+    } else {
+        container "quay.io/biocontainers/ericscript:0.5.5--hdfd78af_5"
+    }
+
+    output:
+    path "*.version.txt" , emit: version
+    path "homo_sapiens/*", emit: reference
+
+    script:
+    def software = getSoftwareName(task.process)
+    """
+    wget https://raw.githubusercontent.com/circulosmeos/gdown.pl/dfd6dc910a38a42d550397bb5c2335be2c4bcf54/gdown.pl && chmod +x gdown.pl
+    ./gdown.pl "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIiUGt1SnFMZFg4TlE" ericscript_db_homosapiens_ensembl84.tar.bz2
+    tar jxf ericscript_db_homosapiens_ensembl84.tar.bz2 --strip-components=2
+    rm ./gdown.pl ericscript_db_homosapiens_ensembl84.tar.bz2
+
+    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
+    """
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/star/genomegenerate/functions.nf b/modules/local/fusioncatcher/detect/functions.nf
similarity index 100%
rename from modules/nf-core/modules/star/genomegenerate/functions.nf
rename to modules/local/fusioncatcher/detect/functions.nf
diff --git a/modules/local/fusioncatcher/detection/main.nf b/modules/local/fusioncatcher/detect/main.nf
similarity index 81%
rename from modules/local/fusioncatcher/detection/main.nf
rename to modules/local/fusioncatcher/detect/main.nf
index 1f2a4405..431d6a8f 100644
--- a/modules/local/fusioncatcher/detection/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -20,32 +20,29 @@ process FUSIONCATCHER {
 
     input:
     tuple val(meta), path(reads)
-    path resource
+    path reference
 
     output:
-    tuple val(meta), path("*.fusioncatcher-fusion-genes.txt")   , optional:true, emit: fusions
-    tuple val(meta), path("*.fusioncatcher_summary.txt")        , optional:true, emit: summary
+    tuple val(meta), path("*.fusioncatcher.fusion-genes.txt")   , optional:true, emit: fusions
+    tuple val(meta), path("*.fusioncatcher.summary.txt")        , optional:true, emit: summary
     tuple val(meta), path("*.fusioncatcher.log")                               , emit: log
     path "*.version.txt"                                                       , emit: version
 
     script:
     def software = getSoftwareName(task.process)
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def fastq    = meta.single_end ? "${reads[0]}" : "${reads[0]},${reads[1]}"
-
     """
     fusioncatcher.py \\
-        -d $resource \\
-        -i $fastq \\
+        -d $reference \\
+        -i $reads \\
         -p $task.cpus \\
         -o . \\
         $options.args
 
-    mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher-fusion-genes.txt
-    mv summary_candidate_fusions.txt ${prefix}.fusioncatcher_summary.txt
+    mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher.fusion-genes.txt
+    mv summary_candidate_fusions.txt ${prefix}.fusioncatcher.summary.txt
     mv fusioncatcher.log ${prefix}.fusioncatcher.log
 
     fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
-
     """
 }
diff --git a/modules/local/fusioncatcher/detection/meta.yml b/modules/local/fusioncatcher/detect/meta.yml
similarity index 100%
rename from modules/local/fusioncatcher/detection/meta.yml
rename to modules/local/fusioncatcher/detect/meta.yml
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 466c055a..ecc5eb83 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -4,12 +4,12 @@ include { initOptions; saveFiles; getSoftwareName } from './functions'
 params.options = [:]
 options        = initOptions(params.options)
 
-process FUSIONCATCHER_DOWNLOADGENOME {
-    tag '$organism'
-    label 'process_low'
+process FUSIONCATCHER_DOWNLOAD {
+    tag 'fusioncatcher'
+    label 'process_medium'
     publishDir "${params.outdir}",
         mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'resources', meta:[:], publish_by_meta:[]) }
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -18,22 +18,28 @@ process FUSIONCATCHER_DOWNLOADGENOME {
         container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_1"
     }
 
-    input:
-    val organism
-
     output:
-    path "fusioncatcher"    , emit: reference
+    path "human_v102"       , emit: reference
     path "*.version.txt"    , emit: version
 
     script:
     def software = getSoftwareName(task.process)
-
+    def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_v102.tar.gz.aa"
     """
-    fusioncatcher-build \\
-        -g $organism \\
-        -o fusioncatcher-genome \\
-        $options.args
-
+    if curl --output /dev/null --silent --head --fail $url; then
+        wget $url \\
+        wget http://sourceforge.net/projects/fusioncatcher/files/data/human_v102.tar.gz.ab
+        wget http://sourceforge.net/projects/fusioncatcher/files/data/human_v102.tar.gz.ac
+        wget http://sourceforge.net/projects/fusioncatcher/files/data/human_v102.tar.gz.ad
+        cat human_v102.tar.gz.* | tar xz
+        rm human_v98.tar*
+    else
+        fusioncatcher-build \\
+            -g homo_sapiens \\
+            -o human_v102 \\
+            $options.args
+    fi
+    
     fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
     """
 }
diff --git a/modules/nf-core/modules/untar/functions.nf b/modules/local/pizzly/detect/functions.nf
similarity index 100%
rename from modules/nf-core/modules/untar/functions.nf
rename to modules/local/pizzly/detect/functions.nf
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
new file mode 100644
index 00000000..b5fb67a8
--- /dev/null
+++ b/modules/local/pizzly/detect/main.nf
@@ -0,0 +1,49 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process PIZZLY {
+    tag "pizzly"
+    label 'process_medium'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "bioconda::kallisto=0.46.2 bioconda::pizzly==0.37.3" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        // FIX: create a multicontainer image
+        container "https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1"
+    } else {
+        // FIX: create a multicontainer image
+        container "quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1"
+    }
+
+    input:
+    tuple val(meta), path(reads)
+    path gtf
+    path index
+    path transcript
+
+    output:
+    path "*.version.txt"                                    , emit: version
+    tuple val(meta), path "${prefix}.pizzly.txt"            , emit: fusions
+    tuple val(meta), path "${prefix}.pizzly.unfiltered.json", emit: fusions_unfiltered
+    
+    script:
+    def software = getSoftwareName(task.process)
+    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    """
+    kallisto quant -t $task.cpus -i $index --fusion -o output $reads
+    pizzly \\
+        $options.args \\
+        --gtf $gtf \\
+        --fasta $transcript \\
+        --output ${prefix}.pizzly output/fusion.txt
+
+    pizzly_flatten_json.py ${prefix}.pizzly.json ${prefix}.pizzly.txt
+
+    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > ${software}.version.txt
+    """
+}
\ No newline at end of file
diff --git a/modules/local/pizzly/download/functions.nf b/modules/local/pizzly/download/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/pizzly/download/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/untar/main.nf b/modules/local/pizzly/download/main.nf
similarity index 54%
rename from modules/nf-core/modules/untar/main.nf
rename to modules/local/pizzly/download/main.nf
index fc6d7ec5..a7468331 100644
--- a/modules/nf-core/modules/untar/main.nf
+++ b/modules/local/pizzly/download/main.nf
@@ -4,32 +4,35 @@ include { initOptions; saveFiles; getSoftwareName } from './functions'
 params.options = [:]
 options        = initOptions(params.options)
 
-process UNTAR {
-    tag "$archive"
-    label 'process_low'
+process PIZZLY_DOWNLOAD {
+    tag "pizzly"
+    label 'process_medium'
     publishDir "${params.outdir}",
         mode: params.publish_dir_mode,
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
-    conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
+    conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img"
+        container "https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1"
     } else {
-        container "biocontainers/biocontainers:v1.2.0_cv1"
+        container "quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1"
     }
 
     input:
-    path archive
+    path transcript
 
     output:
-    path "$untar"       , emit: untar
     path "*.version.txt", emit: version
+    path "index.idx"    , emit: reference
 
     script:
     def software = getSoftwareName(task.process)
-    untar        = archive.toString() - '.tar.gz'
     """
-    tar -xzvf $options.args $archive
-    echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//' > ${software}.version.txt
+    kallisto index \\
+        -i index.idx \\
+        $options.args \\
+        $transcript
+
+    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > ${software}.version.txt
     """
-}
+}
\ No newline at end of file
diff --git a/modules/local/star/align/functions.nf b/modules/local/star/align/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/star/align/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/local/star/align/main.nf
similarity index 95%
rename from modules/nf-core/modules/star/align/main.nf
rename to modules/local/star/align/main.nf
index d5d88ce8..82f1ca93 100644
--- a/modules/nf-core/modules/star/align/main.nf
+++ b/modules/local/star/align/main.nf
@@ -12,11 +12,11 @@ process STAR_ALIGN {
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
+    conda (params.enable_conda ? 'bioconda::star=2.7.8a' : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container 'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0'
+        container 'https://depot.galaxyproject.org/singularity/star:2.7.8a--h9ee0642_1'
     } else {
-        container 'quay.io/biocontainers/star:2.7.9a--h9ee0642_0'
+        container 'quay.io/biocontainers/star:2.7.8a--h9ee0642_1'
     }
 
     input:
diff --git a/modules/nf-core/modules/star/align/meta.yml b/modules/local/star/align/meta.yml
similarity index 100%
rename from modules/nf-core/modules/star/align/meta.yml
rename to modules/local/star/align/meta.yml
diff --git a/modules/local/star/genomegenerate/functions.nf b/modules/local/star/genomegenerate/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/star/genomegenerate/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/local/star/genomegenerate/main.nf b/modules/local/star/genomegenerate/main.nf
new file mode 100644
index 00000000..b01f5753
--- /dev/null
+++ b/modules/local/star/genomegenerate/main.nf
@@ -0,0 +1,47 @@
+// Import generic module functions
+include { initOptions; saveFiles; getSoftwareName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process STAR_GENOMEGENERATE {
+    tag "$fasta"
+    label 'process_high'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+
+    conda (params.enable_conda ? "bioconda::star=2.7.8a" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/star:2.7.8a--h9ee0642_1"
+    } else {
+        container "quay.io/biocontainers/star:2.7.8a--h9ee0642_1"
+    }
+
+    input:
+    path fasta
+    path gtf
+
+    output:
+    path "star"         , emit: index
+    path "*.version.txt", emit: version
+
+    script:
+    def software = getSoftwareName(task.process)
+    def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
+    def args     = options.args.tokenize()
+    """
+    mkdir star
+    STAR \\
+        --runMode genomeGenerate \\
+        --genomeDir star/ \\
+        --genomeFastaFiles $fasta \\
+        --sjdbGTFfile $gtf \\
+        --sjdbOverhang ${params.read_length - 1} \\
+        --runThreadN $task.cpus \\
+        $memory \\
+        $options.args
+
+    STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt
+    """
+}
diff --git a/modules/nf-core/modules/star/genomegenerate/meta.yml b/modules/local/star/genomegenerate/meta.yml
similarity index 100%
rename from modules/nf-core/modules/star/genomegenerate/meta.yml
rename to modules/local/star/genomegenerate/meta.yml
diff --git a/modules/local/starfusion/detect/functions.nf b/modules/local/starfusion/detect/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/local/starfusion/detect/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/local/starfusion/detection/main.nf b/modules/local/starfusion/detect/main.nf
similarity index 56%
rename from modules/local/starfusion/detection/main.nf
rename to modules/local/starfusion/detect/main.nf
index 74317961..2b7570f0 100644
--- a/modules/local/starfusion/detection/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -20,24 +20,35 @@ process STARFUSION {
 
     input:
     tuple val(meta), path(reads)
-    path genome_resource_lib
+    path reference
 
     output:
-    tuple val(meta), path("*.fusion_predictions.tsv")   , emit: fusions
-    path "*.version.txt"                                , emit: version
+    tuple val(meta), path("*.fusion_predictions.tsv"), emit: fusions
+    tuple val(meta), path("*.abridged.tsv")          , emit: abridged
+    tuple val(meta), path("*.coding_effect.tsv")     , optional: true, emit: coding_effect
+    path "*.version.txt"                             , emit: version
 
     script:
     def software    = getSoftwareName(task.process)
-    def prefix      = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def fastq       = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
-
+    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
+    STAR \\
+	    --genomeDir $index \\
+	    --readFilesIn $reads \\
+	    --runThreadN $task.cpus \\
+        $options.args
+
     STAR-Fusion \\
-        --genome_lib_dir $genome_resource_lib \\
-        $fastq \\
+        --genome_lib_dir $reference \\
+        $reads \\
+        --CPU $task.cpus \\
         --output_dir . \\
-        $options.args
+        $options.args2
 
+    mv star-fusion.fusion_predictions.tsv ${prefix}.starfusion.fusion_predictions.tsv
+    mv star-fusion.fusion_predictions.abridged.tsv ${prefix}.starfusion.abridged.tsv
+    mv star-fusion.fusion_predictions.abridged.coding_effect.tsv ${prefix}.starfusion.abridged.coding_effect.tsv
+    
     echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > ${software}.version.txt
     """
 }
diff --git a/modules/local/starfusion/detection/meta.yml b/modules/local/starfusion/detect/meta.yml
similarity index 100%
rename from modules/local/starfusion/detection/meta.yml
rename to modules/local/starfusion/detect/meta.yml
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 2768f686..9758269c 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -4,50 +4,52 @@ include { initOptions; saveFiles; getSoftwareName } from './functions'
 params.options = [:]
 options        = initOptions(params.options)
 
-process STARFUSION_DOWNLOADGENOME {
-    tag 'genome_build'
+process STARFUSION_DOWNLOAD {
+    tag 'star-fusion'
     label 'process_low'
     publishDir "${params.outdir}",
         mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'resources', meta:[:], publish_by_meta:[]) }
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+
+    conda (params.enable_conda ? "bioconda::star-fusion=1.10.0" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/star-fusion:1.10.0--hdfd78af_1"
+    } else {
+        container "quay.io/biocontainers/star-fusion:1.10.0--hdfd78af_1"
+    }
 
     input:
-    val genome_build
+    path fasta
+    path gtf
 
     output:
-    path "star-fusion"  , emit: reference
+    path "*"  , emit: reference
 
     script:
     def software = getSoftwareName(task.process)
-
-    if ( genome_build == "GRCh37" ){
-
-    """
-    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play.tar.gz .
-    mkdir -p star-fusion-genome
-    tar -zxf GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play.tar.gz --strip-components=2 -C star-fusion-genome/
-    rm GRCh37_gencode_v19_CTAT_lib_Mar012021.plug-n-play.tar.gz
-    """
-
-    }
-    else if (genome_build == "GRCh38"){
-
-    """
-    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz .
-    mkdir -p star-fusion-genome
-    tar -zxf GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --strip-components=2 -C star-fusion-genome/
-    rm GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz
-    """
-
-    }
-    else{
-
     """
-    mkdir -p star-fusion-genome
-    echo "Unsupported genome build" > error.log
-    mv error.log star-fusion-genome/
+    export TMPDIR=/tmp
+
+    wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_releases/Pfam-A.hmm.gz
+    wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz -O CTAT_HumanFusionLib_Mar2021.dat.gz
+    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p
+    
+    gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
+
+    prep_genome_lib.pl \\
+        --genome_fa $fasta \\
+        --gtf $gtf \\
+        --annot_filter_rule AnnotFilterRule.pm \\
+        --fusion_annot_lib CTAT_HumanFusionLib_Mar2021.dat.gz \\
+        --pfam_db Pfam-A.hmm \\
+        --dfam_db homo_sapiens_dfam.hmm \\
+        --max_readlength $params.read_length \\
+        --CPU $task.cpus
     """
 
-    }
-
 }
diff --git a/modules/local/starfusion/download/meta.yml b/modules/local/starfusion/download/meta.yml
index ddcf69ee..37f71f06 100644
--- a/modules/local/starfusion/download/meta.yml
+++ b/modules/local/starfusion/download/meta.yml
@@ -14,7 +14,7 @@ tools:
 input:
   - genome_version:
       type: value
-      description: Genome version - either GRCh37 or GRCh38
+      description: Genome version - either GRCh38 or GRCh38
       pattern: "GRCh3*"
 
 output:
diff --git a/modules/nf-core/modules/arriba/meta.yml b/modules/nf-core/modules/arriba/meta.yml
deleted file mode 100644
index 370f82ec..00000000
--- a/modules/nf-core/modules/arriba/meta.yml
+++ /dev/null
@@ -1,54 +0,0 @@
-name: arriba
-description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
-keywords:
-  - fusion
-  - arriba
-tools:
-  - arriba:
-      description: Fast and accurate gene fusion detection from RNA-Seq data
-      homepage: https://github.com/suhrig/arriba
-      documentation: https://arriba.readthedocs.io/en/latest/
-      tool_dev_url: https://github.com/suhrig/arriba
-      doi: "10.1101/gr.257246.119"
-      licence: ['MIT']
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - fasta:
-      type: file
-      description: Assembly FASTA file
-      pattern: "*.{fasta}"
-  - gtf:
-      type: file
-      description: Annotation GTF file
-      pattern: "*.{gtf}"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - version:
-      type: file
-      description: File containing software version
-      pattern: "*.{version.txt}"
-  - fusions:
-      type: file
-      description: File contains fusions which pass all of Arriba's filters.
-      pattern: "*.{fusions.tsv}"
-  - fusions_fail:
-      type: file
-      description: File contains fusions that Arriba classified as an artifact or that are also observed in healthy tissue.
-      pattern: "*.{fusions.discarded.tsv}"
-
-authors:
-  - "@praveenraj2018"
diff --git a/modules/nf-core/modules/gunzip/meta.yml b/modules/nf-core/modules/gunzip/meta.yml
deleted file mode 100644
index 922e74e6..00000000
--- a/modules/nf-core/modules/gunzip/meta.yml
+++ /dev/null
@@ -1,27 +0,0 @@
-name: gunzip
-description: Compresses and decompresses files.
-keywords:
-    - gunzip
-    - compression
-tools:
-    - gunzip:
-        description: |
-            gzip is a file format and a software application used for file compression and decompression.
-        documentation: https://www.gnu.org/software/gzip/manual/gzip.html
-input:
-    - archive:
-        type: file
-        description: File to be compressed/uncompressed
-        pattern: "*.*"
-output:
-    - gunzip:
-        type: file
-        description: Compressed/uncompressed file
-        pattern: "*.*"
-    - version:
-        type: file
-        description: File containing software version
-        pattern: "*.{version.txt}"
-authors:
-    - "@joseespinosa"
-    - "@drpatelh"
diff --git a/modules/nf-core/modules/kallisto/index/functions.nf b/modules/nf-core/modules/kallisto/index/functions.nf
new file mode 100644
index 00000000..da9da093
--- /dev/null
+++ b/modules/nf-core/modules/kallisto/index/functions.nf
@@ -0,0 +1,68 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    if (!args.filename.endsWith('.version.txt')) {
+        def ioptions  = initOptions(args.options)
+        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+        if (ioptions.publish_by_meta) {
+            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+            for (key in key_list) {
+                if (args.meta && key instanceof String) {
+                    def path = key
+                    if (args.meta.containsKey(key)) {
+                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                    }
+                    path = path instanceof String ? path : ''
+                    path_list.add(path)
+                }
+            }
+        }
+        if (ioptions.publish_files instanceof Map) {
+            for (ext in ioptions.publish_files) {
+                if (args.filename.endsWith(ext.key)) {
+                    def ext_list = path_list.collect()
+                    ext_list.add(ext.value)
+                    return "${getPathFromList(ext_list)}/$args.filename"
+                }
+            }
+        } else if (ioptions.publish_files == null) {
+            return "${getPathFromList(path_list)}/$args.filename"
+        }
+    }
+}
diff --git a/modules/nf-core/modules/gunzip/main.nf b/modules/nf-core/modules/kallisto/index/main.nf
similarity index 54%
rename from modules/nf-core/modules/gunzip/main.nf
rename to modules/nf-core/modules/kallisto/index/main.nf
index 29248796..85eb7f0d 100644
--- a/modules/nf-core/modules/gunzip/main.nf
+++ b/modules/nf-core/modules/kallisto/index/main.nf
@@ -4,32 +4,36 @@ include { initOptions; saveFiles; getSoftwareName } from './functions'
 params.options = [:]
 options        = initOptions(params.options)
 
-process GUNZIP {
-    tag "$archive"
-    label 'process_low'
+process KALLISTO_INDEX {
+    tag "$fasta"
+    label 'process_medium'
     publishDir "${params.outdir}",
         mode: params.publish_dir_mode,
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
-    conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
+    conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img"
+        container "https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1"
     } else {
-        container "biocontainers/biocontainers:v1.2.0_cv1"
+        container "quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1"
     }
 
     input:
-    path archive
+    path fasta
 
     output:
-    path "$gunzip",       emit: gunzip
+    path "kallisto" , emit: idx
     path "*.version.txt", emit: version
 
     script:
     def software = getSoftwareName(task.process)
-    gunzip       = archive.toString() - '.gz'
     """
-    gunzip -f $options.args $archive
-    echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//' > ${software}.version.txt
+    kallisto \\
+        index \\
+        $options.args \\
+        -i kallisto \\
+        $fasta
+
+    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > ${software}.version.txt
     """
 }
diff --git a/modules/nf-core/modules/kallisto/index/meta.yml b/modules/nf-core/modules/kallisto/index/meta.yml
new file mode 100644
index 00000000..24b44b0b
--- /dev/null
+++ b/modules/nf-core/modules/kallisto/index/meta.yml
@@ -0,0 +1,31 @@
+name: kallisto_index
+description: write your description here
+keywords:
+  - sort
+tools:
+  - kallisto:
+      description: Quantifying abundances of transcripts from bulk and single-cell RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads.
+      homepage: https://pachterlab.github.io/kallisto/
+      documentation: https://pachterlab.github.io/kallisto/manual
+      tool_dev_url: https://github.com/pachterlab/kallisto
+      doi: ""
+      licence: ['BSD_2_clause']
+
+input:
+  - fasta:
+      type: file
+      description: genome fasta file
+      pattern: "*.{fasta}"
+
+output:
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+  - idx:
+      type: index
+      description: Kallisto genome index
+      pattern: "*.idx"
+
+authors:
+  - "@ggabernet"
diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf
deleted file mode 100644
index 9335b9b5..00000000
--- a/modules/nf-core/modules/star/genomegenerate/main.nf
+++ /dev/null
@@ -1,67 +0,0 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
-process STAR_GENOMEGENERATE {
-    tag "$fasta"
-    label 'process_high'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) }
-
-    // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.13 conda-forge::gawk=5.1.0" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0"
-    } else {
-        container "quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0"
-    }
-
-    input:
-    path fasta
-    path gtf
-
-    output:
-    path "star"         , emit: index
-    path "*.version.txt", emit: version
-
-    script:
-    def software = getSoftwareName(task.process)
-    def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
-    def args     = options.args.tokenize()
-    if (args.contains('--genomeSAindexNbases')) {
-        """
-        mkdir star
-        STAR \\
-            --runMode genomeGenerate \\
-            --genomeDir star/ \\
-            --genomeFastaFiles $fasta \\
-            --sjdbGTFfile $gtf \\
-            --runThreadN $task.cpus \\
-            $memory \\
-            $options.args
-
-        STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt
-        """
-    } else {
-        """
-        samtools faidx $fasta
-        NUM_BASES=`gawk '{sum = sum + \$2}END{if ((log(sum)/log(2))/2 - 1 > 14) {printf "%.0f", 14} else {printf "%.0f", (log(sum)/log(2))/2 - 1}}' ${fasta}.fai`
-
-        mkdir star
-        STAR \\
-            --runMode genomeGenerate \\
-            --genomeDir star/ \\
-            --genomeFastaFiles $fasta \\
-            --sjdbGTFfile $gtf \\
-            --runThreadN $task.cpus \\
-            --genomeSAindexNbases \$NUM_BASES \\
-            $memory \\
-            $options.args
-
-        STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt
-        """
-    }
-}
diff --git a/modules/nf-core/modules/untar/meta.yml b/modules/nf-core/modules/untar/meta.yml
deleted file mode 100644
index af4674f0..00000000
--- a/modules/nf-core/modules/untar/meta.yml
+++ /dev/null
@@ -1,27 +0,0 @@
-name: untar
-description: Extract files.
-keywords:
-    - untar
-    - uncompress
-tools:
-    - untar:
-        description: |
-            Extract tar.gz files.
-        documentation: https://www.gnu.org/software/tar/manual/
-input:
-    - archive:
-        type: file
-        description: File to be untar
-        pattern: "*.{tar}.{gz}"
-output:
-    - untar:
-        type: file
-        description:
-        pattern: "*.*"
-    - version:
-        type: file
-        description: File containing software version
-        pattern: "*.{version.txt}"
-authors:
-    - "@joseespinosa"
-    - "@drpatelh"
diff --git a/nextflow.config b/nextflow.config
index 67f5d55d..fce81b40 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -12,35 +12,30 @@ params {
     // Input options
     input                      = null
 
-    // References
-    genome                     = 'GRCh38'
-    igenomes_base              = 's3://ngi-igenomes/igenomes'
-    igenomes_ignore            = false
-    save_reference             = false
-    aligner                    = 'star'
-
     // MultiQC options
     multiqc_config             = null
     multiqc_title              = null
     max_multiqc_email_size     = '25.MB'
 
-    //Alignment options
+    // Genome
+    genome                     = 'GRCh38'
+    ensembl_version            = 97
+    read_length                = 100
+
+    // Alignment options
     star_ignore_sjdbgtf        = false
     seq_center                 = null
     seq_platform               = null
     star_index                 = null
 
-    //Enable or disable tools
-    arriba                     = true
-    starfusion                 = false
-    fusioncatcher              = false
+    // Enable or disable tools
+    all                        = false
+    arriba                     = false
     ericscript                 = false
-    squid                      = false
+    fusioncatcher              = false
     pizzly                     = false
-
-    //genome resources
-    starfusion_genome          = null
-    fusioncatcher_genome       = null
+    squid                      = false
+    starfusion                 = false
 
 
     // Boilerplate options
@@ -87,13 +82,6 @@ try {
     System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
 }
 
-// Load igenomes.config if required
-if (!params.igenomes_ignore) {
-    includeConfig 'conf/igenomes.config'
-} else {
-    params.genomes = [:]
-}
-
 profiles {
     debug { process.beforeScript = 'echo $HOSTNAME' }
     conda {
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 7ae5077d..13359495 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -50,12 +50,6 @@
             "fa_icon": "fas fa-dna",
             "description": "Reference genome related files and options required for the workflow.",
             "properties": {
-                "genome": {
-                    "type": "string",
-                    "description": "Name of iGenomes reference.",
-                    "fa_icon": "fas fa-book",
-                    "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details."
-                },
                 "fasta": {
                     "type": "string",
                     "format": "file-path",
@@ -64,21 +58,6 @@
                     "description": "Path to FASTA genome file.",
                     "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.",
                     "fa_icon": "far fa-file-code"
-                },
-                "igenomes_base": {
-                    "type": "string",
-                    "format": "directory-path",
-                    "description": "Directory / URL base for iGenomes references.",
-                    "default": "s3://ngi-igenomes/igenomes",
-                    "fa_icon": "fas fa-cloud-download-alt",
-                    "hidden": true
-                },
-                "igenomes_ignore": {
-                    "type": "boolean",
-                    "description": "Do not load the iGenomes reference config.",
-                    "fa_icon": "fas fa-ban",
-                    "hidden": true,
-                    "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
                 }
             }
         },
@@ -268,13 +247,6 @@
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues."
                 },
-                "save_reference": {
-                    "type": "boolean",
-                    "description": "Whether to save the reference or not.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Save the reference for future executions."
-                },
                 "seq_center": {
                     "type": "boolean",
                     "description": "Sequencing center",
@@ -289,13 +261,6 @@
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "This will reported in the BAM header as PL."
                 },
-                "aligner": {
-                    "type": "string",
-                    "description": "List of read aligners to be used",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Specify the aligned to be used for RNAseq alignment"
-                },
                 "star_ignore_sjdbgtf": {
                     "type": "boolean",
                     "description": "Whether to ignore the GTF in STAR alignment",
@@ -309,62 +274,6 @@
                     "hidden": true,
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "Provide the index directory for the STAR alignment"
-                },
-                "starfusion_genome": {
-                    "type": "string",
-                    "description": "Path of the genome resource directory of STAR-Fusion",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Provide the genome resource data for the STAR-Fusion"
-                },
-                "fusioncatcher_genome": {
-                    "type": "string",
-                    "description": "Path of the genome resource directory of FusionCatcher",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Provide the path of the resource data path for FusionCatcher"
-                },
-                "arriba": {
-                    "type": "boolean",
-                    "description": "List of read aligners to be used",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Specify whether to run arriba or not"
-                },
-                "starfusion": {
-                    "type": "boolean",
-                    "description": "List of read aligners to be used",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Specify whether to run starfusion or not"
-                },
-                "fusioncatcher": {
-                    "type": "boolean",
-                    "description": "List of read aligners to be used",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Specify whether to run fusioncatcher or not"
-                },
-                "ericscript": {
-                    "type": "boolean",
-                    "description": "List of read aligners to be used",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Specify whether to run ericscript or not"
-                },
-                "squid": {
-                    "type": "boolean",
-                    "description": "List of read aligners to be used",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Specify whether to run squid or not"
-                },
-                "pizzly": {
-                    "type": "boolean",
-                    "description": "List of read aligners to be used",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Specify whether to run pizzly or not"
                 }
             }
         }
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
new file mode 100644
index 00000000..0ec24cda
--- /dev/null
+++ b/workflows/build_references.nf
@@ -0,0 +1,68 @@
+/*
+========================================================================================
+    IMPORT LOCAL MODULES/SUBWORKFLOWS
+========================================================================================
+*/
+
+// Don't overwrite global params.modules, create a copy instead and use that within the main script.
+def modules = params.modules.clone()
+
+include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'                    addParams( options: modules['ensembl_download'] )
+include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'            addParams( options: modules['arriba_download'] )
+include { STAR_GENOMEGENERATE }             from '../modules/local/star/genomegenerate/main'        addParams( options: modules['star_index'] )
+include { ERICSCRIPT_DOWNLOAD }             from '../modules/local/ericscript/download/main'        addParams( options: modules['ericscript_download'] )
+include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'     addParams( options: modules['fusioncatcher_download'] )
+include { KALLISTO_INDEX as PIZZLY_INDEX }  from '../modules/nf-core/modules/kallisto/index/main'   addParams( options: modules['pizzly_download'] )
+include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'        addParams( options: modules['starfusion_download'] )
+
+/*
+========================================================================================
+    RUN MAIN WORKFLOW
+========================================================================================
+*/
+
+workflow BUILD_REFERENCES {
+
+    ENSEMBL_DOWNLOAD( params.ensembl_version )
+
+    STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
+
+    if (params.arriba || params.all) {
+        ARRIBA_DOWNLOAD()
+    }
+
+    if (params.ericscript || params.all) {
+        ERICSCRIPT_DOWNLOAD()
+    }
+
+    if (params.fusioncatcher || params.all) {
+        FUSIONCATCHER_DOWNLOAD()
+    }
+
+    if (params.pizzly || params.all) {
+        PIZZLY_INDEX( ENSEMBL_DOWNLOAD.out.transcript )
+    }
+
+    if (params.starfusion || params.all) {
+        STARFUSION_DOWNLOAD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
+    }
+
+}
+
+/*
+========================================================================================
+    COMPLETION EMAIL AND SUMMARY
+========================================================================================
+*/
+
+workflow.onComplete {
+    def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
+    // NfcoreTemplate.email(workflow, params, summary_params, projectDir, log)
+    NfcoreTemplate.summary(workflow, params, log)
+}
+
+/*
+========================================================================================
+    THE END
+========================================================================================
+*/
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 0d35afc7..db8075d2 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -20,10 +20,6 @@ for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true
 // Check mandatory parameters
 if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' }
 
-// Check alignment parameters
-def prepareToolIndices  = []
-prepareToolIndices << params.aligner
-
 /*
 ========================================================================================
     CONFIG FILES
@@ -41,12 +37,9 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 
 // Don't overwrite global params.modules, create a copy instead and use that within the main script.
 def modules = params.modules.clone()
-def publish_genome_options = params.save_reference ? [publish_dir: 'genome']       : [publish_files: false]
-def publish_index_options  = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false]
 def star_genomegenerate_options = modules['star_genomegenerate']
 def starfusion_genome_options = modules['starfusion_download']
 def fusioncatcher_genome_options = modules['fusioncatcher_download']
-if (!params.save_reference)     { star_genomegenerate_options['publish_files'] = false }
 
 // MODULE: Local to the pipeline
 //
@@ -55,7 +48,6 @@ include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions'
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
 include { INPUT_CHECK } from '../subworkflows/local/input_check'                addParams( options: [:] )
-include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome'          addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options, starfusion_untar_options: starfusion_genome_options, starfusion_download_options: starfusion_genome_options, fusioncatcher_download_options: fusioncatcher_genome_options)
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
@@ -85,11 +77,6 @@ def multiqc_report = []
 
 workflow RNAFUSION {
 
-    // SUBWORKFLOW: Uncompress and prepare reference genome files
-    PREPARE_GENOME (
-        prepareToolIndices
-    )
-
     ch_software_versions = Channel.empty()
     ch_reads = Channel.empty()
     //

From 8f99894eeb5cd1cb7745d923dc709c3a57feaf43 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Mon, 4 Oct 2021 09:48:10 +0200
Subject: [PATCH 048/359] fix: using Pfamv34

---
 modules/local/starfusion/download/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 9758269c..bae796e5 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -30,7 +30,7 @@ process STARFUSION_DOWNLOAD {
     """
     export TMPDIR=/tmp
 
-    wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_releases/Pfam-A.hmm.gz
+    wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz
     wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz -O CTAT_HumanFusionLib_Mar2021.dat.gz
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm

From 6e192fa5a13b4d06802f217f1951b1785efe8226 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Mon, 4 Oct 2021 09:48:47 +0200
Subject: [PATCH 049/359] fix: extra wget params if download fails

---
 conf/modules.config | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index fd8cf9da..d1674d9e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -51,7 +51,8 @@ params {
             args                = ""
         }
         'fusioncatcher_download' {
-            args                = ""
+            args                = "--continue --tries=42"
+            args2               = ""
             publish_dir         = "references/${params.genome}/fusioncatcher"
         }
         'pizzly' {

From 679e46e5071b7963e6b61bedba5dc51134cc403b Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Mon, 4 Oct 2021 09:56:51 +0200
Subject: [PATCH 050/359] fix: optional star-index build

---
 nextflow.config               | 1 +
 workflows/build_references.nf | 4 +++-
 2 files changed, 4 insertions(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index fce81b40..04abc9b7 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -35,6 +35,7 @@ params {
     fusioncatcher              = false
     pizzly                     = false
     squid                      = false
+    starindex                  = false
     starfusion                 = false
 
 
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 0ec24cda..65fc812b 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -25,7 +25,9 @@ workflow BUILD_REFERENCES {
 
     ENSEMBL_DOWNLOAD( params.ensembl_version )
 
-    STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
+    if (params.starindex || params.all) {
+        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
+    }
 
     if (params.arriba || params.all) {
         ARRIBA_DOWNLOAD()

From 0b8dec5fdd18dea558dd57ccc35e1a61bd2457e1 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Sun, 5 Dec 2021 15:29:42 +0100
Subject: [PATCH 051/359] fix: ericscript download from iGenomes

---
 modules/local/ericscript/download/main.nf | 14 +++++++-------
 1 file changed, 7 insertions(+), 7 deletions(-)

diff --git a/modules/local/ericscript/download/main.nf b/modules/local/ericscript/download/main.nf
index 86307736..2248997e 100644
--- a/modules/local/ericscript/download/main.nf
+++ b/modules/local/ericscript/download/main.nf
@@ -11,11 +11,11 @@ process ERICSCRIPT_DOWNLOAD {
         mode: params.publish_dir_mode,
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
-    conda (params.enable_conda ? "bioconda::ericscript=0.5.5" : null)
+    conda (params.enable_conda ? "conda-forge::awscli" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/ericscript:0.5.5--hdfd78af_5"
+        container "https://depot.galaxyproject.org/singularity/python:3.7"
     } else {
-        container "quay.io/biocontainers/ericscript:0.5.5--hdfd78af_5"
+        container "quay.io/bitnami/python:3.7"
     }
 
     output:
@@ -25,11 +25,11 @@ process ERICSCRIPT_DOWNLOAD {
     script:
     def software = getSoftwareName(task.process)
     """
-    wget https://raw.githubusercontent.com/circulosmeos/gdown.pl/dfd6dc910a38a42d550397bb5c2335be2c4bcf54/gdown.pl && chmod +x gdown.pl
-    ./gdown.pl "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIiUGt1SnFMZFg4TlE" ericscript_db_homosapiens_ensembl84.tar.bz2
+    pip install awscli
+    aws s3 --no-sign-request --region eu-west-1 sync s3://ngi-igenomes/igenomes/Homo_sapiens/Ensembl/GRCh38/Sequence/ericscript_db_homosapiens_ensembl84.tar.bz2 .
     tar jxf ericscript_db_homosapiens_ensembl84.tar.bz2 --strip-components=2
-    rm ./gdown.pl ericscript_db_homosapiens_ensembl84.tar.bz2
+    rm ericscript_db_homosapiens_ensembl84.tar.bz2
 
-    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
+    echo \$(aws --version 2>&1) cut -d "/" -f 2 | cut -d " " -f 1 > ${software}.version.txt
     """
 }
\ No newline at end of file

From ad8f86fd4aec08dc87209b7fc94d31ebdb92b248 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Sun, 5 Dec 2021 15:31:03 +0100
Subject: [PATCH 052/359] fix: download or build fusioncatcher

---
 modules/local/fusioncatcher/download/main.nf | 27 ++++++++++----------
 1 file changed, 14 insertions(+), 13 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index ecc5eb83..dbb3b8d5 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -19,27 +19,28 @@ process FUSIONCATCHER_DOWNLOAD {
     }
 
     output:
-    path "human_v102"       , emit: reference
-    path "*.version.txt"    , emit: version
+    path "human_*"      , emit: reference
+    path "*.version.txt", emit: version
 
     script:
     def software = getSoftwareName(task.process)
-    def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_v102.tar.gz.aa"
+    def human_version = "v102"
+    def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.aa"
     """
-    if curl --output /dev/null --silent --head --fail $url; then
-        wget $url \\
-        wget http://sourceforge.net/projects/fusioncatcher/files/data/human_v102.tar.gz.ab
-        wget http://sourceforge.net/projects/fusioncatcher/files/data/human_v102.tar.gz.ac
-        wget http://sourceforge.net/projects/fusioncatcher/files/data/human_v102.tar.gz.ad
-        cat human_v102.tar.gz.* | tar xz
-        rm human_v98.tar*
+    if wget --spider "$url" 2>/dev/null; then
+        wget $options.args $url
+        wget $options.args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ab
+        wget $options.args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ac
+        wget $options.args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ad
+        cat human_${human_version}.tar.gz.* | tar xz
+        rm human_${human_version}.tar*
     else
         fusioncatcher-build \\
             -g homo_sapiens \\
-            -o human_v102 \\
-            $options.args
+            -o human_${human_version} \\
+            $options.args2
     fi
-    
+
     fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
     """
 }

From 9ff4171e0c122b457cb86a9a969b064c807c088e Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Tue, 7 Dec 2021 14:42:55 +0100
Subject: [PATCH 053/359] feat: star-fusion mulled container

---
 modules/local/starfusion/detect/main.nf   | 6 +++---
 modules/local/starfusion/download/main.nf | 6 +++---
 2 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 2b7570f0..bf219417 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -11,11 +11,11 @@ process STARFUSION {
         mode: params.publish_dir_mode,
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
-    conda (params.enable_conda ? "bioconda::star-fusion=1.10.0" : null)
+    conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/star-fusion:1.10.0--hdfd78af_1"
+        container "https://depot.galaxyproject.org/singularity/mulled-v2-57582e8bdbf51679bdcff9de91ae016a44e322de:b978a3a1e8715581329a267a2c9904574384180a-0"
     } else {
-        container "quay.io/biocontainers/star-fusion:1.10.0--hdfd78af_1"
+        container "quay.io/biocontainers/mulled-v2-57582e8bdbf51679bdcff9de91ae016a44e322de:b978a3a1e8715581329a267a2c9904574384180a-0"
     }
 
     input:
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index bae796e5..86044ef0 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -11,11 +11,11 @@ process STARFUSION_DOWNLOAD {
         mode: params.publish_dir_mode,
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
-    conda (params.enable_conda ? "bioconda::star-fusion=1.10.0" : null)
+    conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/star-fusion:1.10.0--hdfd78af_1"
+        container "https://depot.galaxyproject.org/singularity/mulled-v2-57582e8bdbf51679bdcff9de91ae016a44e322de:b978a3a1e8715581329a267a2c9904574384180a-0"
     } else {
-        container "quay.io/biocontainers/star-fusion:1.10.0--hdfd78af_1"
+        container "quay.io/biocontainers/mulled-v2-57582e8bdbf51679bdcff9de91ae016a44e322de:b978a3a1e8715581329a267a2c9904574384180a-0"
     }
 
     input:

From 5c133a3de710703af00de6a9ab1ac69bfae5be52 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Sun, 12 Dec 2021 13:34:48 +0100
Subject: [PATCH 054/359] fix: aws `sync` replaced with `cp` for download

---
 modules/local/ericscript/download/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/ericscript/download/main.nf b/modules/local/ericscript/download/main.nf
index 2248997e..d3f15981 100644
--- a/modules/local/ericscript/download/main.nf
+++ b/modules/local/ericscript/download/main.nf
@@ -26,7 +26,7 @@ process ERICSCRIPT_DOWNLOAD {
     def software = getSoftwareName(task.process)
     """
     pip install awscli
-    aws s3 --no-sign-request --region eu-west-1 sync s3://ngi-igenomes/igenomes/Homo_sapiens/Ensembl/GRCh38/Sequence/ericscript_db_homosapiens_ensembl84.tar.bz2 .
+    aws s3 --no-sign-request --region eu-west-1 cp s3://ngi-igenomes/igenomes/Homo_sapiens/Ensembl/GRCh38/Sequence/ericscript_db_homosapiens_ensembl84.tar.bz2 .
     tar jxf ericscript_db_homosapiens_ensembl84.tar.bz2 --strip-components=2
     rm ericscript_db_homosapiens_ensembl84.tar.bz2
 

From 49811f8302f249e1fa79416fd1cce1dca587d2c2 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Sun, 12 Dec 2021 17:26:16 +0100
Subject: [PATCH 055/359] fix: aws `sync` replaced with wget for download

---
 modules/local/ericscript/download/main.nf | 11 +++++------
 1 file changed, 5 insertions(+), 6 deletions(-)

diff --git a/modules/local/ericscript/download/main.nf b/modules/local/ericscript/download/main.nf
index d3f15981..5ea73970 100644
--- a/modules/local/ericscript/download/main.nf
+++ b/modules/local/ericscript/download/main.nf
@@ -11,11 +11,11 @@ process ERICSCRIPT_DOWNLOAD {
         mode: params.publish_dir_mode,
         saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
-    conda (params.enable_conda ? "conda-forge::awscli" : null)
+    conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/python:3.7"
+        container "https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5"
     } else {
-        container "quay.io/bitnami/python:3.7"
+        container "quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5"
     }
 
     output:
@@ -25,11 +25,10 @@ process ERICSCRIPT_DOWNLOAD {
     script:
     def software = getSoftwareName(task.process)
     """
-    pip install awscli
-    aws s3 --no-sign-request --region eu-west-1 cp s3://ngi-igenomes/igenomes/Homo_sapiens/Ensembl/GRCh38/Sequence/ericscript_db_homosapiens_ensembl84.tar.bz2 .
+    wget http://ngi-igenomes.s3.amazonaws.com/igenomes/Homo_sapiens/Ensembl/GRCh38/Sequence/ericscript_db_homosapiens_ensembl84.tar.bz2
     tar jxf ericscript_db_homosapiens_ensembl84.tar.bz2 --strip-components=2
     rm ericscript_db_homosapiens_ensembl84.tar.bz2
 
-    echo \$(aws --version 2>&1) cut -d "/" -f 2 | cut -d " " -f 1 > ${software}.version.txt
+    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
     """
 }
\ No newline at end of file

From 7e6bc98211ff54236b7d448f11d338222228c237 Mon Sep 17 00:00:00 2001
From: Martin Proks <mproksik@gmail.com>
Date: Sun, 12 Dec 2021 17:27:04 +0100
Subject: [PATCH 056/359] fix: added missing `genomes_base` param

---
 nextflow.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/nextflow.config b/nextflow.config
index 04abc9b7..a87af0da 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -19,6 +19,7 @@ params {
 
     // Genome
     genome                     = 'GRCh38'
+    genomes_base               = null
     ensembl_version            = 97
     read_length                = 100
 

From f80bcde7a2ff48c874683e1838916023d2b5e285 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 14 Dec 2021 16:42:39 +0000
Subject: [PATCH 057/359] Template update for nf-core/tools version 2.2

---
 .gitattributes                                |   2 +
 .github/CONTRIBUTING.md                       |  38 ++------
 .github/ISSUE_TEMPLATE/bug_report.md          |  63 -------------
 .github/ISSUE_TEMPLATE/bug_report.yml         |  52 ++++++++++
 .github/ISSUE_TEMPLATE/config.yml             |   1 -
 .github/ISSUE_TEMPLATE/feature_request.md     |  32 -------
 .github/ISSUE_TEMPLATE/feature_request.yml    |  11 +++
 .github/workflows/awsfulltest.yml             |   8 +-
 .github/workflows/awstest.yml                 |  10 +-
 .github/workflows/ci.yml                      |  23 +++--
 .github/workflows/linting_comment.yml         |   1 +
 CITATIONS.md                                  |   2 +-
 README.md                                     |  13 ++-
 assets/multiqc_config.yaml                    |   2 +-
 assets/nf-core-rnafusion_logo.png             | Bin 18138 -> 0 bytes
 assets/nf-core-rnafusion_logo_light.png       |   9 ++
 assets/sendmail_template.txt                  |   4 +-
 bin/scrape_software_versions.py               |  36 -------
 conf/base.config                              |   3 +
 conf/modules.config                           |  55 ++++++-----
 conf/test.config                              |   4 +-
 docs/images/nf-core-rnafusion_logo.png        | Bin 35022 -> 0 bytes
 docs/images/nf-core-rnafusion_logo_dark.png   |   9 ++
 docs/images/nf-core-rnafusion_logo_light.png  |   9 ++
 docs/output.md                                |   2 +-
 docs/usage.md                                 |  36 -------
 lib/NfcoreSchema.groovy                       |  26 +++--
 lib/NfcoreTemplate.groovy                     |  30 ++----
 lib/Utils.groovy                              |   7 --
 lib/WorkflowMain.groovy                       |   6 +-
 modules.json                                  |   9 +-
 modules/local/functions.nf                    |  68 -------------
 modules/local/get_software_versions.nf        |  33 -------
 modules/local/samplesheet_check.nf            |  24 ++---
 .../custom/dumpsoftwareversions/main.nf       |  21 +++++
 .../custom/dumpsoftwareversions/meta.yml      |  34 +++++++
 .../templates/dumpsoftwareversions.py         |  89 ++++++++++++++++++
 modules/nf-core/modules/fastqc/functions.nf   |  68 -------------
 modules/nf-core/modules/fastqc/main.nf        |  39 ++++----
 modules/nf-core/modules/fastqc/meta.yml       |   7 +-
 modules/nf-core/modules/multiqc/functions.nf  |  68 -------------
 modules/nf-core/modules/multiqc/main.nf       |  31 +++---
 modules/nf-core/modules/multiqc/meta.yml      |   7 +-
 nextflow.config                               |  31 +++---
 nextflow_schema.json                          |  29 ------
 subworkflows/local/input_check.nf             |   8 +-
 workflows/rnafusion.nf                        |  44 +++------
 47 files changed, 435 insertions(+), 669 deletions(-)
 delete mode 100644 .github/ISSUE_TEMPLATE/bug_report.md
 create mode 100644 .github/ISSUE_TEMPLATE/bug_report.yml
 delete mode 100644 .github/ISSUE_TEMPLATE/feature_request.md
 create mode 100644 .github/ISSUE_TEMPLATE/feature_request.yml
 delete mode 100644 assets/nf-core-rnafusion_logo.png
 create mode 100644 assets/nf-core-rnafusion_logo_light.png
 delete mode 100755 bin/scrape_software_versions.py
 delete mode 100644 docs/images/nf-core-rnafusion_logo.png
 create mode 100644 docs/images/nf-core-rnafusion_logo_dark.png
 create mode 100644 docs/images/nf-core-rnafusion_logo_light.png
 delete mode 100644 modules/local/functions.nf
 delete mode 100644 modules/local/get_software_versions.nf
 create mode 100644 modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
 create mode 100644 modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
 create mode 100644 modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
 delete mode 100644 modules/nf-core/modules/fastqc/functions.nf
 delete mode 100644 modules/nf-core/modules/multiqc/functions.nf

diff --git a/.gitattributes b/.gitattributes
index 7fe55006..050bb120 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -1 +1,3 @@
 *.config linguist-language=nextflow
+modules/nf-core/** linguist-generated
+subworkflows/nf-core/** linguist-generated
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 1912ed9d..4dfbe44c 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -68,16 +68,13 @@ If you wish to contribute a new step, please use the following coding standards:
 1. Define the corresponding input channel into your new process from the expected previous process channel
 2. Write the process block (see below).
 3. Define the output channel if needed (see below).
-4. Add any new flags/options to `nextflow.config` with a default (see below).
-5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build`).
-6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter).
-7. Add sanity checks for all relevant parameters.
-8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`.
-9. Do local tests that the new code works properly and as expected.
-10. Add a new test command in `.github/workflow/ci.yml`.
-11. If applicable add a [MultiQC](https://https://multiqc.info/) module.
-12. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, name clean up, General Statistics Table column order, and module figures are in the right order.
-13. Optional: Add any descriptions of MultiQC report sections and output files to `docs/output.md`.
+4. Add any new parameters to `nextflow.config` with a default (see below).
+5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core schema build` tool).
+6. Add sanity checks and validation for all relevant parameters.
+7. Perform local tests to validate that the new code works as expected.
+8. If applicable, add a new test command in `.github/workflow/ci.yml`.
+9. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module.
+10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`.
 
 ### Default values
 
@@ -102,27 +99,6 @@ Please use the following naming schemes, to make it easy to understand what is g
 
 If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]`
 
-### Software version reporting
-
-If you add a new tool to the pipeline, please ensure you add the information of the tool to the `get_software_version` process.
-
-Add to the script block of the process, something like the following:
-
-```bash
-<YOUR_TOOL> --version &> v_<YOUR_TOOL>.txt 2>&1 || true
-```
-
-or
-
-```bash
-<YOUR_TOOL> --help | head -n 1 &> v_<YOUR_TOOL>.txt 2>&1 || true
-```
-
-You then need to edit the script `bin/scrape_software_versions.py` to:
-
-1. Add a Python regex for your tool's `--version` output (as in stored in the `v_<YOUR_TOOL>.txt` file), to ensure the version is reported as a `v` and the version number e.g. `v2.1.1`
-2. Add a HTML entry to the `OrderedDict` for formatting in MultiQC.
-
 ### Images and figures
 
 For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines).
diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md
deleted file mode 100644
index 21b44f28..00000000
--- a/.github/ISSUE_TEMPLATE/bug_report.md
+++ /dev/null
@@ -1,63 +0,0 @@
----
-name: Bug report
-about: Report something that is broken or incorrect
-labels: bug
----
-
-<!--
-# nf-core/rnafusion bug report
-
-Hi there!
-
-Thanks for telling us about a problem with the pipeline.
-Please delete this text and anything that's not relevant from the template below:
--->
-
-## Check Documentation
-
-I have checked the following places for your error:
-
-- [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
-- [ ] [nf-core/rnafusion pipeline documentation](https://nf-co.re/rnafusion/usage)
-
-## Description of the bug
-
-<!-- A clear and concise description of what the bug is. -->
-
-## Steps to reproduce
-
-Steps to reproduce the behaviour:
-
-1. Command line: <!-- [e.g. `nextflow run ...`] -->
-2. See error: <!-- [Please provide your error message] -->
-
-## Expected behaviour
-
-<!-- A clear and concise description of what you expected to happen. -->
-
-## Log files
-
-Have you provided the following extra information/files:
-
-- [ ] The command used to run the pipeline
-- [ ] The `.nextflow.log` file <!-- this is a hidden file in the directory where you launched the pipeline -->
-
-## System
-
-- Hardware: <!-- [e.g. HPC, Desktop, Cloud...] -->
-- Executor: <!-- [e.g. slurm, local, awsbatch...] -->
-- OS: <!-- [e.g. CentOS Linux, macOS, Linux Mint...] -->
-- Version <!-- [e.g. 7, 10.13.6, 18.3...] -->
-
-## Nextflow Installation
-
-- Version: <!-- [e.g. 21.04.0] -->
-
-## Container engine
-
-- Engine: <!-- [e.g. Conda, Docker, Singularity, Podman, Shifter or Charliecloud] -->
-- version: <!-- [e.g. 1.0.0] -->
-
-## Additional context
-
-<!-- Add any other context about the problem here. -->
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
new file mode 100644
index 00000000..a3de1c24
--- /dev/null
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -0,0 +1,52 @@
+
+name: Bug report
+description: Report something that is broken or incorrect
+labels: bug
+body:
+
+  - type: markdown
+    attributes:
+      value: |
+        Before you post this issue, please check the documentation:
+
+        - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
+        - [nf-core/rnafusion pipeline documentation](https://nf-co.re/rnafusion/usage)
+
+  - type: textarea
+    id: description
+    attributes:
+      label: Description of the bug
+      description: A clear and concise description of what the bug is.
+    validations:
+      required: true
+
+  - type: textarea
+    id: command_used
+    attributes:
+      label: Command used and terminal output
+      description: Steps to reproduce the behaviour. Please paste the command you used to launch the pipeline and the output from your terminal.
+      render: console
+      placeholder: |
+        $ nextflow run ...
+
+        Some output where something broke
+
+  - type: textarea
+    id: files
+    attributes:
+      label: Relevant files
+      description: |
+        Please drag and drop the relevant files here. Create a `.zip` archive if the extension is not allowed.
+        Your verbose log file `.nextflow.log` is often useful _(this is a hidden file in the directory where you launched the pipeline)_ as well as custom Nextflow configuration files.
+
+  - type: textarea
+    id: system
+    attributes:
+      label: System information
+      description: |
+        * Nextflow version _(eg. 21.10.3)_
+        * Hardware _(eg. HPC, Desktop, Cloud)_
+        * Executor _(eg. slurm, local, awsbatch)_
+        * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_
+        * OS _(eg. CentOS Linux, macOS, Linux Mint)_
+        * Version of nf-core/rnafusion _(eg. 1.1, 1.5, 1.8.2)_
diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml
index 1fccc741..69a065e7 100644
--- a/.github/ISSUE_TEMPLATE/config.yml
+++ b/.github/ISSUE_TEMPLATE/config.yml
@@ -1,4 +1,3 @@
-blank_issues_enabled: false
 contact_links:
   - name: Join nf-core
     url: https://nf-co.re/join
diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md
deleted file mode 100644
index 10da2c83..00000000
--- a/.github/ISSUE_TEMPLATE/feature_request.md
+++ /dev/null
@@ -1,32 +0,0 @@
----
-name: Feature request
-about: Suggest an idea for the nf-core/rnafusion pipeline
-labels: enhancement
----
-
-<!--
-# nf-core/rnafusion feature request
-
-Hi there!
-
-Thanks for suggesting a new feature for the pipeline!
-Please delete this text and anything that's not relevant from the template below:
--->
-
-## Is your feature request related to a problem? Please describe
-
-<!-- A clear and concise description of what the problem is. -->
-
-<!-- e.g. [I'm always frustrated when ...] -->
-
-## Describe the solution you'd like
-
-<!-- A clear and concise description of what you want to happen. -->
-
-## Describe alternatives you've considered
-
-<!-- A clear and concise description of any alternative solutions or features you've considered. -->
-
-## Additional context
-
-<!-- Add any other context about the feature request here. -->
diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml
new file mode 100644
index 00000000..2c388d2f
--- /dev/null
+++ b/.github/ISSUE_TEMPLATE/feature_request.yml
@@ -0,0 +1,11 @@
+name: Feature request
+description: Suggest an idea for the nf-core/rnafusion pipeline
+labels: enhancement
+body:
+  - type: textarea
+    id: description
+    attributes:
+      label: Description of feature
+      description: Please describe your suggestion for a new feature. It might help to describe a problem or use case, plus any alternatives that you have considered.
+    validations:
+      required: true
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index ed90a293..64316f92 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -14,14 +14,14 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@master
+        uses: nf-core/tower-action@v2
         # TODO nf-core: You can customise AWS full pipeline tests as required
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
         
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
-          bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }}
+          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
           revision: ${{ github.sha }}
@@ -30,5 +30,5 @@ jobs:
             {
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
             }
-          profiles: '[ "test_full", "aws_tower" ]'
-
+          profiles: test_full,aws_tower
+          pre_run_script: 'export NXF_VER=21.10.3'
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index c3b9fbb4..2c933fcc 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -11,18 +11,18 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@master
+        uses: nf-core/tower-action@v2
         
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
-          bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }}
+          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
           revision: ${{ github.sha }}
           workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
           parameters: |
             {
-              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-test-${{ github.sha }}"
             }
-          profiles: '[ "test", "aws_tower" ]'
-
+          profiles: test,aws_tower
+          pre_run_script: 'export NXF_VER=21.10.3'
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 1145652b..f9ff5247 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -8,8 +8,9 @@ on:
   release:
     types: [published]
 
-# Uncomment if we need an edge release of Nextflow again
-# env: NXF_EDGE: 1
+env:
+  NXF_ANSI_LOG: false
+  CAPSULE_LOG: none
 
 jobs:
   test:
@@ -17,20 +18,26 @@ jobs:
     # Only run on push if this is the nf-core dev branch (merged PRs)
     if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}
     runs-on: ubuntu-latest
-    env:
-      NXF_VER: ${{ matrix.nxf_ver }}
-      NXF_ANSI_LOG: false
     strategy:
       matrix:
-        # Nextflow versions: check pipeline minimum and current latest
-        nxf_ver: ['21.04.0', '']
+        # Nextflow versions
+        include:
+          # Test pipeline minimum Nextflow version
+          - NXF_VER: '21.10.3'
+            NXF_EDGE: ''
+          # Test latest edge release of Nextflow
+          - NXF_VER: ''
+            NXF_EDGE: '1'
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
 
       - name: Install Nextflow
         env:
-          CAPSULE_LOG: none
+          NXF_VER: ${{ matrix.NXF_VER }}
+          # Uncomment only if the edge release is more recent than the latest stable release
+          # See https://github.com/nextflow-io/nextflow/issues/2467
+          # NXF_EDGE: ${{ matrix.NXF_EDGE }}
         run: |
           wget -qO- get.nextflow.io | bash
           sudo mv nextflow /usr/local/bin/
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 90f03c6f..44d72994 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -15,6 +15,7 @@ jobs:
         uses: dawidd6/action-download-artifact@v2
         with:
           workflow: linting.yml
+          workflow_conclusion: completed
 
       - name: Get PR number
         id: pr_number
diff --git a/CITATIONS.md b/CITATIONS.md
index 9b38bf50..702b64c9 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -12,7 +12,7 @@
 
 * [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
 
-* [MultiQC](https://www.ncbi.nlm.nih.gov/pubmed/27312411/)
+* [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
     > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
 
 ## Software packaging/containerisation tools
diff --git a/README.md b/README.md
index f65ac7b7..23fffa60 100644
--- a/README.md
+++ b/README.md
@@ -1,11 +1,11 @@
-# ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo.png)
+# ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_light.png#gh-light-mode-only) ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_dark.png#gh-dark-mode-only)
 
 [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+CI%22)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)
 [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)
 [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 
-[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.04.0-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
@@ -33,18 +33,21 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 ## Quick Start
 
-1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.04.0`)
+1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`)
 
 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
 
 3. Download the pipeline and test it on a minimal dataset with a single command:
 
     ```console
-    nextflow run nf-core/rnafusion -profile test,<docker/singularity/podman/shifter/charliecloud/conda/institute>
+    nextflow run nf-core/rnafusion -profile test,YOURPROFILE
     ```
 
+    Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
+
+    > * The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
     > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
-    > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, it is highly recommended to use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to pre-download all of the required containers before running the pipeline and to set the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options to be able to store and re-use the images from a central location for future pipeline runs.
+    > * If you are using `singularity` and are persistently observing issues downloading Singularity images directly due to timeout or network issues, then you can use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, you can use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
     > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
 
 4. Start running your own analysis!
diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml
index 14f8ada3..611287cc 100644
--- a/assets/multiqc_config.yaml
+++ b/assets/multiqc_config.yaml
@@ -1,7 +1,7 @@
 report_comment: >
     This report has been generated by the <a href="https://github.com/nf-core/rnafusion" target="_blank">nf-core/rnafusion</a>
     analysis pipeline. For information about how to interpret these results, please see the
-    <a href="https://github.com/nf-core/rnafusion" target="_blank">documentation</a>.
+    <a href="https://nf-co.re/rnafusion" target="_blank">documentation</a>.
 report_section_order:
     software_versions:
         order: -1000
diff --git a/assets/nf-core-rnafusion_logo.png b/assets/nf-core-rnafusion_logo.png
deleted file mode 100644
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diff --git a/assets/nf-core-rnafusion_logo_light.png b/assets/nf-core-rnafusion_logo_light.png
new file mode 100644
index 00000000..dee3db82
--- /dev/null
+++ b/assets/nf-core-rnafusion_logo_light.png
@@ -0,0 +1,9 @@
+
+<!DOCTYPE HTML PUBLIC "-//IETF//DTD HTML 2.0//EN">
+<HTML><HEAD>
+<TITLE> 508 Resource Limit Is Reached</TITLE>
+</HEAD><BODY>
+<H1>Resource Limit Is Reached</H1>
+The website is temporarily unable to service your request as it exceeded resource limit.
+Please try again later.
+</BODY></HTML>
diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt
index 06728832..ff6631ad 100644
--- a/assets/sendmail_template.txt
+++ b/assets/sendmail_template.txt
@@ -12,9 +12,9 @@ $email_html
 Content-Type: image/png;name="nf-core-rnafusion_logo.png"
 Content-Transfer-Encoding: base64
 Content-ID: <nfcorepipelinelogo>
-Content-Disposition: inline; filename="nf-core-rnafusion_logo.png"
+Content-Disposition: inline; filename="nf-core-rnafusion_logo_light.png"
 
-<% out << new File("$projectDir/assets/nf-core-rnafusion_logo.png").
+<% out << new File("$projectDir/assets/nf-core-rnafusion_logo_light.png").
     bytes.
     encodeBase64().
     toString().
diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
deleted file mode 100755
index ee132097..00000000
--- a/bin/scrape_software_versions.py
+++ /dev/null
@@ -1,36 +0,0 @@
-#!/usr/bin/env python
-from __future__ import print_function
-import os
-
-results = {}
-version_files = [x for x in os.listdir(".") if x.endswith(".version.txt")]
-for version_file in version_files:
-
-    software = version_file.replace(".version.txt", "")
-    if software == "pipeline":
-        software = "nf-core/rnafusion"
-
-    with open(version_file) as fin:
-        version = fin.read().strip()
-    results[software] = version
-
-# Dump to YAML
-print(
-    """
-id: 'software_versions'
-section_name: 'nf-core/rnafusion Software Versions'
-section_href: 'https://github.com/nf-core/rnafusion'
-plot_type: 'html'
-description: 'are collected at run time from the software output.'
-data: |
-    <dl class="dl-horizontal">
-"""
-)
-for k, v in sorted(results.items()):
-    print("        <dt>{}</dt><dd><samp>{}</samp></dd>".format(k, v))
-print("    </dl>")
-
-# Write out as tsv file:
-with open("software_versions.tsv", "w") as f:
-    for k, v in sorted(results.items()):
-        f.write("{}\t{}\n".format(k, v))
diff --git a/conf/base.config b/conf/base.config
index 5619f83a..f2d66794 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -54,4 +54,7 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
+    withName:CUSTOM_DUMPSOFTWAREVERSIONS {
+        cache = false
+    }
 }
diff --git a/conf/modules.config b/conf/modules.config
index 0b1bfdec..a0506a4d 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -1,32 +1,41 @@
 /*
 ========================================================================================
-    Config file for defining DSL2 per module options
+    Config file for defining DSL2 per module options and publishing paths
 ========================================================================================
     Available keys to override module options:
-        args            = Additional arguments appended to command in module.
-        args2           = Second set of arguments appended to command in module (multi-tool modules).
-        args3           = Third set of arguments appended to command in module (multi-tool modules).
-        publish_dir     = Directory to publish results.
-        publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path
-                            If publish_by_meta = true                 - Value of ${meta['id']} is appended as a directory to "publish_dir" path
-                            If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/"
-                                                                        is appended as a directory to "publish_dir" path
-                            If publish_by_meta = false / null         - No directories are appended to "publish_dir" path
-        publish_files   = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension
-                            The value of "directory" is appended to the standard "publish_dir" path as defined above.
-                            If publish_files = null (unspecified)     - All files are published.
-                            If publish_files = false                  - No files are published.
-        suffix          = File name suffix for output files.
+        ext.args            = Additional arguments appended to command in module.
+        ext.args2           = Second set of arguments appended to command in module (multi-tool modules).
+        ext.args3           = Third set of arguments appended to command in module (multi-tool modules).
+        ext.prefix          = File name prefix for output files.
 ----------------------------------------------------------------------------------------
 */
 
-params {
-    modules {
-        'fastqc' {
-            args = "--quiet"
-        }
-        'multiqc' {
-            args = ""
-        }
+process {
+
+    publishDir = [
+        path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
+        mode: 'copy',
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+    ]
+
+    withName: SAMPLESHEET_CHECK {
+        publishDir = [
+            path: { "${params.outdir}/pipeline_info" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
+    withName: FASTQC {
+        ext.args = '--quiet'
     }
+
+    withName: CUSTOM_DUMPSOFTWAREVERSIONS {
+        publishDir = [
+            path: { "${params.outdir}/pipeline_info" },
+            mode: 'copy',
+            pattern: '*_versions.yml'
+        ]
+    }
+
 }
diff --git a/conf/test.config b/conf/test.config
index 331ff129..d10f8c89 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -16,8 +16,8 @@ params {
 
     // Limit resources so that this can run on GitHub Actions
     max_cpus   = 2
-    max_memory = 6.GB
-    max_time   = 6.h
+    max_memory = '6.GB'
+    max_time   = '6.h'
 
     // Input data
     // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
diff --git a/docs/images/nf-core-rnafusion_logo.png b/docs/images/nf-core-rnafusion_logo.png
deleted file mode 100644
index 97835e0eb03020a770579e936da155e9fddb0f90..0000000000000000000000000000000000000000
GIT binary patch
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diff --git a/docs/images/nf-core-rnafusion_logo_dark.png b/docs/images/nf-core-rnafusion_logo_dark.png
new file mode 100644
index 00000000..dee3db82
--- /dev/null
+++ b/docs/images/nf-core-rnafusion_logo_dark.png
@@ -0,0 +1,9 @@
+
+<!DOCTYPE HTML PUBLIC "-//IETF//DTD HTML 2.0//EN">
+<HTML><HEAD>
+<TITLE> 508 Resource Limit Is Reached</TITLE>
+</HEAD><BODY>
+<H1>Resource Limit Is Reached</H1>
+The website is temporarily unable to service your request as it exceeded resource limit.
+Please try again later.
+</BODY></HTML>
diff --git a/docs/images/nf-core-rnafusion_logo_light.png b/docs/images/nf-core-rnafusion_logo_light.png
new file mode 100644
index 00000000..dee3db82
--- /dev/null
+++ b/docs/images/nf-core-rnafusion_logo_light.png
@@ -0,0 +1,9 @@
+
+<!DOCTYPE HTML PUBLIC "-//IETF//DTD HTML 2.0//EN">
+<HTML><HEAD>
+<TITLE> 508 Resource Limit Is Reached</TITLE>
+</HEAD><BODY>
+<H1>Resource Limit Is Reached</H1>
+The website is temporarily unable to service your request as it exceeded resource limit.
+Please try again later.
+</BODY></HTML>
diff --git a/docs/output.md b/docs/output.md
index 8a3fc362..06979c38 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -60,7 +60,7 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ
 
 * `pipeline_info/`
     * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
-    * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.tsv`.
+    * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
     * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
 
 </details>
diff --git a/docs/usage.md b/docs/usage.md
index 7f14f65b..0126fca9 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -181,42 +181,6 @@ process {
 
 > **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow.
 
-### Tool-specific options
-
-For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes.
-
-The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of:
-
-* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file.
-
-* An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option.
-
-The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments.
-
-When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`.
-
-As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file.
-
-Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config.
-
-As you will see in the example below, we have:
-
-* appended `--outFilterMismatchNmax 16` to the default `args` used by the module.
-* changed the default `publish_dir` value to where the files will eventually be published in the main results directory.
-* appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`.
-
-```nextflow
-params {
-    modules {
-        'star_align' {
-            args          = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16"
-            publish_dir   = "my_star_directory"
-            publish_files = ['out':'log', 'tab':'log', 'bam':'']
-        }
-    }
-}
-```
-
 ### Updating containers
 
 The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`.
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index 8d6920dd..40ab65f2 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -105,9 +105,13 @@ class NfcoreSchema {
 
         // Collect expected parameters from the schema
         def expectedParams = []
+        def enums = [:]
         for (group in schemaParams) {
             for (p in group.value['properties']) {
                 expectedParams.push(p.key)
+                if (group.value['properties'][p.key].containsKey('enum')) {
+                    enums[p.key] = group.value['properties'][p.key]['enum']
+                }
             }
         }
 
@@ -155,7 +159,7 @@ class NfcoreSchema {
             println ''
             log.error 'ERROR: Validation of pipeline parameters failed!'
             JSONObject exceptionJSON = e.toJSON()
-            printExceptions(exceptionJSON, params_json, log)
+            printExceptions(exceptionJSON, params_json, log, enums)
             println ''
             has_error = true
         }
@@ -202,7 +206,7 @@ class NfcoreSchema {
                 }
                 def type = '[' + group_params.get(param).type + ']'
                 def description = group_params.get(param).description
-                def defaultValue = group_params.get(param).default ? " [default: " + group_params.get(param).default.toString() + "]" : ''
+                def defaultValue = group_params.get(param).default != null ? " [default: " + group_params.get(param).default.toString() + "]" : ''
                 def description_default = description + colors.dim + defaultValue + colors.reset
                 // Wrap long description texts
                 // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap
@@ -260,13 +264,12 @@ class NfcoreSchema {
 
         // Get pipeline parameters defined in JSON Schema
         def Map params_summary = [:]
-        def blacklist  = ['hostnames']
         def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename))
         for (group in params_map.keySet()) {
             def sub_params = new LinkedHashMap()
             def group_params = params_map.get(group)  // This gets the parameters of that particular group
             for (param in group_params.keySet()) {
-                if (params.containsKey(param) && !blacklist.contains(param)) {
+                if (params.containsKey(param)) {
                     def params_value = params.get(param)
                     def schema_value = group_params.get(param).default
                     def param_type   = group_params.get(param).type
@@ -330,7 +333,7 @@ class NfcoreSchema {
     //
     // Loop over nested exceptions and print the causingException
     //
-    private static void printExceptions(ex_json, params_json, log) {
+    private static void printExceptions(ex_json, params_json, log, enums, limit=5) {
         def causingExceptions = ex_json['causingExceptions']
         if (causingExceptions.length() == 0) {
             def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/
@@ -346,11 +349,20 @@ class NfcoreSchema {
             else {
                 def param = ex_json['pointerToViolation'] - ~/^#\//
                 def param_val = params_json[param].toString()
-                log.error "* --${param}: ${ex_json['message']} (${param_val})"
+                if (enums.containsKey(param)) {
+                    def error_msg = "* --${param}: '${param_val}' is not a valid choice (Available choices"
+                    if (enums[param].size() > limit) {
+                        log.error "${error_msg} (${limit} of ${enums[param].size()}): ${enums[param][0..limit-1].join(', ')}, ... )"
+                    } else {
+                        log.error "${error_msg}: ${enums[param].join(', ')})"
+                    }
+                } else {
+                    log.error "* --${param}: ${ex_json['message']} (${param_val})"
+                }
             }
         }
         for (ex in causingExceptions) {
-            printExceptions(ex, params_json, log)
+            printExceptions(ex, params_json, log, enums)
         }
     }
 
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index 44551e0a..2fc0a9b9 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -19,27 +19,16 @@ class NfcoreTemplate {
     }
 
     //
-    // Check params.hostnames
+    //  Warn if a -profile or Nextflow config has not been provided to run the pipeline
     //
-    public static void hostName(workflow, params, log) {
-        Map colors = logColours(params.monochrome_logs)
-        if (params.hostnames) {
-            try {
-                def hostname = "hostname".execute().text.trim()
-                params.hostnames.each { prof, hnames ->
-                    hnames.each { hname ->
-                        if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                            log.info "=${colors.yellow}====================================================${colors.reset}=\n" +
-                                "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" +
-                                "      but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" +
-                                "      ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" +
-                                "=${colors.yellow}====================================================${colors.reset}="
-                        }
-                    }
-                }
-            } catch (Exception e) {
-                log.warn "[$workflow.manifest.name] Could not determine 'hostname' - skipping check. Reason: ${e.message}."
-            }
+    public static void checkConfigProvided(workflow, log) {
+        if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
+            log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
+                    "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
+                    "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
+                    "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
+                    "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
+                    "Please refer to the quick start section and usage docs for the pipeline.\n "
         }
     }
 
@@ -168,7 +157,6 @@ class NfcoreTemplate {
                 log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
             }
         } else {
-            hostName(workflow, params, log)
             log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
         }
     }
diff --git a/lib/Utils.groovy b/lib/Utils.groovy
index 18173e98..1b88aec0 100755
--- a/lib/Utils.groovy
+++ b/lib/Utils.groovy
@@ -37,11 +37,4 @@ class Utils {
                 "==================================================================================="
         }
     }
-
-    //
-    // Join module args with appropriate spacing
-    //
-    public static String joinModuleArgs(args_list) {
-        return ' ' + args_list.join(' ')
-    }
 }
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index 4caed266..6f431e59 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -61,6 +61,9 @@ class WorkflowMain {
         // Print parameter summary log to screen
         log.info paramsSummaryLog(workflow, params, log)
 
+        // Check that a -profile or Nextflow config has been provided to run the pipeline
+        NfcoreTemplate.checkConfigProvided(workflow, log)
+
         // Check that conda channels are set-up correctly
         if (params.enable_conda) {
             Utils.checkCondaChannels(log)
@@ -69,9 +72,6 @@ class WorkflowMain {
         // Check AWS batch settings
         NfcoreTemplate.awsBatch(workflow, params)
 
-        // Check the hostnames against configured profiles
-        NfcoreTemplate.hostName(workflow, params, log)
-
         // Check input has been provided
         if (!params.input) {
             log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'"
diff --git a/modules.json b/modules.json
index 5e4e806f..0e469950 100644
--- a/modules.json
+++ b/modules.json
@@ -3,12 +3,15 @@
     "homePage": "https://github.com/nf-core/rnafusion",
     "repos": {
         "nf-core/modules": {
+            "custom/dumpsoftwareversions": {
+                "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41"
+            },
             "fastqc": {
-                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+                "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961"
             },
             "multiqc": {
-                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+                "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41"
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/local/functions.nf b/modules/local/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/get_software_versions.nf b/modules/local/get_software_versions.nf
deleted file mode 100644
index 2568f170..00000000
--- a/modules/local/get_software_versions.nf
+++ /dev/null
@@ -1,33 +0,0 @@
-// Import generic module functions
-include { saveFiles } from './functions'
-
-params.options = [:]
-
-process GET_SOFTWARE_VERSIONS {
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) }
-
-    conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/python:3.8.3"
-    } else {
-        container "quay.io/biocontainers/python:3.8.3"
-    }
-
-    cache false
-
-    input:
-    path versions
-
-    output:
-    path "software_versions.tsv"     , emit: tsv
-    path 'software_versions_mqc.yaml', emit: yaml
-
-    script: // This script is bundled with the pipeline, in nf-core/rnafusion/bin/
-    """
-    echo $workflow.manifest.version > pipeline.version.txt
-    echo $workflow.nextflow.version > nextflow.version.txt
-    scrape_software_versions.py &> software_versions_mqc.yaml
-    """
-}
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index 5cff46fe..9ed59266 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -1,31 +1,27 @@
-// Import generic module functions
-include { saveFiles } from './functions'
-
-params.options = [:]
-
 process SAMPLESHEET_CHECK {
     tag "$samplesheet"
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/python:3.8.3"
-    } else {
-        container "quay.io/biocontainers/python:3.8.3"
-    }
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/python:3.8.3' :
+        'quay.io/biocontainers/python:3.8.3' }"
 
     input:
     path samplesheet
 
     output:
-    path '*.csv'
+    path '*.csv'       , emit: csv
+    path "versions.yml", emit: versions
 
     script: // This script is bundled with the pipeline, in nf-core/rnafusion/bin/
     """
     check_samplesheet.py \\
         $samplesheet \\
         samplesheet.valid.csv
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        python: \$(python --version | sed 's/Python //g')
+    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
new file mode 100644
index 00000000..934bb467
--- /dev/null
+++ b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
@@ -0,0 +1,21 @@
+process CUSTOM_DUMPSOFTWAREVERSIONS {
+    label 'process_low'
+
+    // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
+    conda (params.enable_conda ? "bioconda::multiqc=1.11" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }"
+
+    input:
+    path versions
+
+    output:
+    path "software_versions.yml"    , emit: yml
+    path "software_versions_mqc.yml", emit: mqc_yml
+    path "versions.yml"             , emit: versions
+
+    script:
+    def args = task.ext.args ?: ''
+    template 'dumpsoftwareversions.py'
+}
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
new file mode 100644
index 00000000..5b5b8a60
--- /dev/null
+++ b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
@@ -0,0 +1,34 @@
+name: custom_dumpsoftwareversions
+description: Custom module used to dump software versions within the nf-core pipeline template
+keywords:
+  - custom
+  - version
+tools:
+  - custom:
+      description: Custom module used to dump software versions within the nf-core pipeline template
+      homepage: https://github.com/nf-core/tools
+      documentation: https://github.com/nf-core/tools
+      licence: ['MIT']
+input:
+  - versions:
+      type: file
+      description: YML file containing software versions
+      pattern: "*.yml"
+
+output:
+  - yml:
+      type: file
+      description: Standard YML file containing software versions
+      pattern: "software_versions.yml"
+  - mqc_yml:
+      type: file
+      description: MultiQC custom content YML file containing software versions
+      pattern: "software_versions_mqc.yml"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@drpatelh"
+  - "@grst"
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
new file mode 100644
index 00000000..d1390392
--- /dev/null
+++ b/modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
@@ -0,0 +1,89 @@
+#!/usr/bin/env python
+
+import yaml
+import platform
+from textwrap import dedent
+
+
+def _make_versions_html(versions):
+    html = [
+        dedent(
+            """\\
+            <style>
+            #nf-core-versions tbody:nth-child(even) {
+                background-color: #f2f2f2;
+            }
+            </style>
+            <table class="table" style="width:100%" id="nf-core-versions">
+                <thead>
+                    <tr>
+                        <th> Process Name </th>
+                        <th> Software </th>
+                        <th> Version  </th>
+                    </tr>
+                </thead>
+            """
+        )
+    ]
+    for process, tmp_versions in sorted(versions.items()):
+        html.append("<tbody>")
+        for i, (tool, version) in enumerate(sorted(tmp_versions.items())):
+            html.append(
+                dedent(
+                    f"""\\
+                    <tr>
+                        <td><samp>{process if (i == 0) else ''}</samp></td>
+                        <td><samp>{tool}</samp></td>
+                        <td><samp>{version}</samp></td>
+                    </tr>
+                    """
+                )
+            )
+        html.append("</tbody>")
+    html.append("</table>")
+    return "\\n".join(html)
+
+
+versions_this_module = {}
+versions_this_module["${task.process}"] = {
+    "python": platform.python_version(),
+    "yaml": yaml.__version__,
+}
+
+with open("$versions") as f:
+    versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
+
+# aggregate versions by the module name (derived from fully-qualified process name)
+versions_by_module = {}
+for process, process_versions in versions_by_process.items():
+    module = process.split(":")[-1]
+    try:
+        assert versions_by_module[module] == process_versions, (
+            "We assume that software versions are the same between all modules. "
+            "If you see this error-message it means you discovered an edge-case "
+            "and should open an issue in nf-core/tools. "
+        )
+    except KeyError:
+        versions_by_module[module] = process_versions
+
+versions_by_module["Workflow"] = {
+    "Nextflow": "$workflow.nextflow.version",
+    "$workflow.manifest.name": "$workflow.manifest.version",
+}
+
+versions_mqc = {
+    "id": "software_versions",
+    "section_name": "${workflow.manifest.name} Software Versions",
+    "section_href": "https://github.com/${workflow.manifest.name}",
+    "plot_type": "html",
+    "description": "are collected at run time from the software output.",
+    "data": _make_versions_html(versions_by_module),
+}
+
+with open("software_versions.yml", "w") as f:
+    yaml.dump(versions_by_module, f, default_flow_style=False)
+with open("software_versions_mqc.yml", "w") as f:
+    yaml.dump(versions_mqc, f, default_flow_style=False)
+
+with open("versions.yml", "w") as f:
+    yaml.dump(versions_this_module, f, default_flow_style=False)
diff --git a/modules/nf-core/modules/fastqc/functions.nf b/modules/nf-core/modules/fastqc/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/nf-core/modules/fastqc/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
index 39c327b2..d250eca0 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -1,22 +1,11 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process FASTQC {
     tag "$meta.id"
     label 'process_medium'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0"
-    } else {
-        container "quay.io/biocontainers/fastqc:0.11.9--0"
-    }
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' :
+        'quay.io/biocontainers/fastqc:0.11.9--0' }"
 
     input:
     tuple val(meta), path(reads)
@@ -24,24 +13,32 @@ process FASTQC {
     output:
     tuple val(meta), path("*.html"), emit: html
     tuple val(meta), path("*.zip") , emit: zip
-    path  "*.version.txt"          , emit: version
+    path  "versions.yml"           , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     // Add soft-links to original FastQs for consistent naming in pipeline
-    def software = getSoftwareName(task.process)
-    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def prefix = task.ext.prefix ?: "${meta.id}"
     if (meta.single_end) {
         """
         [ ! -f  ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
-        fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz
-        fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
+        fastqc $args --threads $task.cpus ${prefix}.fastq.gz
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        END_VERSIONS
         """
     } else {
         """
         [ ! -f  ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
         [ ! -f  ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
-        fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
-        fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
+        fastqc $args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        END_VERSIONS
         """
     }
 }
diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml
index 8eb9953d..b09553a3 100644
--- a/modules/nf-core/modules/fastqc/meta.yml
+++ b/modules/nf-core/modules/fastqc/meta.yml
@@ -15,6 +15,7 @@ tools:
             overrepresented sequences.
         homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
         documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
+        licence: ['GPL-2.0-only']
 input:
     - meta:
         type: map
@@ -40,10 +41,10 @@ output:
         type: file
         description: FastQC report archive
         pattern: "*_{fastqc.zip}"
-    - version:
+    - versions:
         type: file
-        description: File containing software version
-        pattern: "*.{version.txt}"
+        description: File containing software versions
+        pattern: "versions.yml"
 authors:
     - "@drpatelh"
     - "@grst"
diff --git a/modules/nf-core/modules/multiqc/functions.nf b/modules/nf-core/modules/multiqc/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/nf-core/modules/multiqc/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index da780800..3dceb162 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -1,21 +1,10 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process MULTIQC {
     label 'process_medium'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
-    conda (params.enable_conda ? "bioconda::multiqc=1.10.1" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/multiqc:1.10.1--py_0"
-    } else {
-        container "quay.io/biocontainers/multiqc:1.10.1--py_0"
-    }
+    conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }"
 
     input:
     path multiqc_files
@@ -24,12 +13,16 @@ process MULTIQC {
     path "*multiqc_report.html", emit: report
     path "*_data"              , emit: data
     path "*_plots"             , optional:true, emit: plots
-    path "*.version.txt"       , emit: version
+    path "versions.yml"        , emit: versions
 
     script:
-    def software = getSoftwareName(task.process)
+    def args = task.ext.args ?: ''
     """
-    multiqc -f $options.args .
-    multiqc --version | sed -e "s/multiqc, version //g" > ${software}.version.txt
+    multiqc -f $args .
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
+    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml
index 532a8bb1..63c75a45 100644
--- a/modules/nf-core/modules/multiqc/meta.yml
+++ b/modules/nf-core/modules/multiqc/meta.yml
@@ -11,6 +11,7 @@ tools:
             It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
         homepage: https://multiqc.info/
         documentation: https://multiqc.info/docs/
+        licence: ['GPL-3.0-or-later']
 input:
     - multiqc_files:
         type: file
@@ -29,10 +30,10 @@ output:
         type: file
         description: Plots created by MultiQC
         pattern: "*_data"
-    - version:
+    - versions:
         type: file
-        description: File containing software version
-        pattern: "*.{version.txt}"
+        description: File containing software versions
+        pattern: "versions.yml"
 authors:
     - "@abhi18av"
     - "@bunop"
diff --git a/nextflow.config b/nextflow.config
index 118c2479..497e7ea2 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -26,7 +26,6 @@ params {
     // Boilerplate options
     outdir                     = './results'
     tracedir                   = "${params.outdir}/pipeline_info"
-    publish_dir_mode           = 'copy'
     email                      = null
     email_on_fail              = null
     plaintext_email            = false
@@ -34,14 +33,12 @@ params {
     help                       = false
     validate_params            = true
     show_hidden_params         = false
-    schema_ignore_params       = 'genomes,modules'
+    schema_ignore_params       = 'genomes'
     enable_conda               = false
-    singularity_pull_docker_container = false
 
     // Config options
     custom_config_version      = 'master'
     custom_config_base         = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
-    hostnames                  = [:]
     config_profile_description = null
     config_profile_contact     = null
     config_profile_url         = null
@@ -58,9 +55,6 @@ params {
 // Load base.config by default for all pipelines
 includeConfig 'conf/base.config'
 
-// Load modules.config for DSL2 module specific options
-includeConfig 'conf/modules.config'
-
 // Load nf-core custom profiles from different Institutions
 try {
     includeConfig "${params.custom_config_base}/nfcore_custom.config"
@@ -68,13 +62,6 @@ try {
     System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
 }
 
-// Load igenomes.config if required
-if (!params.igenomes_ignore) {
-    includeConfig 'conf/igenomes.config'
-} else {
-    params.genomes = [:]
-}
-
 profiles {
     debug { process.beforeScript = 'echo $HOSTNAME' }
     conda {
@@ -126,11 +113,22 @@ profiles {
     test_full { includeConfig 'conf/test_full.config' }
 }
 
+// Load igenomes.config if required
+if (!params.igenomes_ignore) {
+    includeConfig 'conf/igenomes.config'
+} else {
+    params.genomes = [:]
+}
+
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container
+// The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. 
+// See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable.
+
 env {
     PYTHONNOUSERSITE = 1
     R_PROFILE_USER   = "/.Rprofile"
     R_ENVIRON_USER   = "/.Renviron"
+    JULIA_DEPOT_PATH = "/usr/local/share/julia"
 }
 
 // Capture exit codes from upstream processes when piping
@@ -160,10 +158,13 @@ manifest {
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
     mainScript      = 'main.nf'
-    nextflowVersion = '!>=21.04.0'
+    nextflowVersion = '!>=21.10.3'
     version         = '1.3.0dev'
 }
 
+// Load modules.config for DSL2 module specific options
+includeConfig 'conf/modules.config'
+
 // Function to ensure that resource requirements don't go beyond
 // a maximum limit
 def check_max(obj, type) {
diff --git a/nextflow_schema.json b/nextflow_schema.json
index af484875..f602f89c 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -104,12 +104,6 @@
                     "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.",
                     "fa_icon": "fas fa-users-cog"
                 },
-                "hostnames": {
-                    "type": "string",
-                    "description": "Institutional configs hostname.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
-                },
                 "config_profile_name": {
                     "type": "string",
                     "description": "Institutional config name.",
@@ -184,22 +178,6 @@
                     "fa_icon": "fas fa-question-circle",
                     "hidden": true
                 },
-                "publish_dir_mode": {
-                    "type": "string",
-                    "default": "copy",
-                    "description": "Method used to save pipeline results to output directory.",
-                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
-                    "fa_icon": "fas fa-copy",
-                    "enum": [
-                        "symlink",
-                        "rellink",
-                        "link",
-                        "copy",
-                        "copyNoFollow",
-                        "move"
-                    ],
-                    "hidden": true
-                },
                 "email_on_fail": {
                     "type": "string",
                     "description": "Email address for completion summary, only when pipeline fails.",
@@ -260,13 +238,6 @@
                     "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.",
                     "hidden": true,
                     "fa_icon": "fas fa-bacon"
-                },
-                "singularity_pull_docker_container": {
-                    "type": "boolean",
-                    "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues."
                 }
             }
         }
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index b664bc8c..cddcbb3c 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -2,9 +2,7 @@
 // Check input samplesheet and get read channels
 //
 
-params.options = [:]
-
-include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options )
+include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check'
 
 workflow INPUT_CHECK {
     take:
@@ -12,12 +10,14 @@ workflow INPUT_CHECK {
 
     main:
     SAMPLESHEET_CHECK ( samplesheet )
+        .csv
         .splitCsv ( header:true, sep:',' )
         .map { create_fastq_channels(it) }
         .set { reads }
 
     emit:
-    reads // channel: [ val(meta), [ reads ] ]
+    reads                                     // channel: [ val(meta), [ reads ] ]
+    versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ]
 }
 
 // Function to get list of [ meta, [ fastq_1, fastq_2 ] ]
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 10879efe..4dad9839 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -32,18 +32,10 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 ========================================================================================
 */
 
-// Don't overwrite global params.modules, create a copy instead and use that within the main script.
-def modules = params.modules.clone()
-
-//
-// MODULE: Local to the pipeline
-//
-include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] )
-
 //
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
-include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] )
+include { INPUT_CHECK } from '../subworkflows/local/input_check'
 
 /*
 ========================================================================================
@@ -51,14 +43,12 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( opti
 ========================================================================================
 */
 
-def multiqc_options   = modules['multiqc']
-multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : ''
-
 //
 // MODULE: Installed directly from nf-core/modules
 //
-include { FASTQC  } from '../modules/nf-core/modules/fastqc/main'  addParams( options: modules['fastqc'] )
-include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options   )
+include { FASTQC                      } from '../modules/nf-core/modules/fastqc/main'
+include { MULTIQC                     } from '../modules/nf-core/modules/multiqc/main'
+include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main'
 
 /*
 ========================================================================================
@@ -71,7 +61,7 @@ def multiqc_report = []
 
 workflow RNAFUSION {
 
-    ch_software_versions = Channel.empty()
+    ch_versions = Channel.empty()
 
     //
     // SUBWORKFLOW: Read in samplesheet, validate and stage input files
@@ -79,6 +69,7 @@ workflow RNAFUSION {
     INPUT_CHECK (
         ch_input
     )
+    ch_versions = ch_versions.mix(INPUT_CHECK.out.versions)
 
     //
     // MODULE: Run FastQC
@@ -86,21 +77,10 @@ workflow RNAFUSION {
     FASTQC (
         INPUT_CHECK.out.reads
     )
-    ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null))
+    ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
-    //
-    // MODULE: Pipeline reporting
-    //
-    ch_software_versions
-        .map { it -> if (it) [ it.baseName, it ] }
-        .groupTuple()
-        .map { it[1][0] }
-        .flatten()
-        .collect()
-        .set { ch_software_versions }
-
-    GET_SOFTWARE_VERSIONS (
-        ch_software_versions.map { it }.collect()
+    CUSTOM_DUMPSOFTWAREVERSIONS (
+        ch_versions.unique().collectFile(name: 'collated_versions.yml')
     )
 
     //
@@ -113,14 +93,14 @@ workflow RNAFUSION {
     ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
     ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
     ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect())
+    ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
     ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
 
     MULTIQC (
         ch_multiqc_files.collect()
     )
-    multiqc_report       = MULTIQC.out.report.toList()
-    ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null))
+    multiqc_report = MULTIQC.out.report.toList()
+    ch_versions    = ch_versions.mix(MULTIQC.out.versions)
 }
 
 /*

From 79a1d3c6372329c3b7104cb71524685fed35c0e2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 10:51:29 +0100
Subject: [PATCH 058/359] publish star index results

---
 modules/local/star/genomegenerate/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/star/genomegenerate/main.nf b/modules/local/star/genomegenerate/main.nf
index b01f5753..1eb4c0f4 100644
--- a/modules/local/star/genomegenerate/main.nf
+++ b/modules/local/star/genomegenerate/main.nf
@@ -23,8 +23,8 @@ process STAR_GENOMEGENERATE {
     path gtf
 
     output:
-    path "star"         , emit: index
-    path "*.version.txt", emit: version
+    path "star/*"         , emit: index
+    path "*.version.txt"  , emit: version
 
     script:
     def software = getSoftwareName(task.process)

From af1d897c33951ca16426175e69cbc4291c5e436f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 11:02:38 +0100
Subject: [PATCH 059/359] Exchange gtf with chr.gtf

---
 modules/local/ensembl/main.nf | 10 +++++-----
 1 file changed, 5 insertions(+), 5 deletions(-)

diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 42f9a33c..08949eeb 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -24,7 +24,7 @@ process ENSEMBL_DOWNLOAD {
     output:
     path "*.version.txt"                                                  , emit: version
     path "Homo_sapiens.${params.genome}.${ensembl_version}.all.fa"        , emit: fasta
-    path "Homo_sapiens.${params.genome}.${ensembl_version}.gtf"           , emit: gtf
+    path "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"       , emit: gtf
     path "Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz", emit: transcript
 
     script:
@@ -33,12 +33,12 @@ process ENSEMBL_DOWNLOAD {
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
 
-    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
+    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz
 
     gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa
-    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
-    
+    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
+
     echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
     """
-}
\ No newline at end of file
+}

From bc026fd6df83b1ced14245b7235a2803af4a0a0a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 11:03:43 +0100
Subject: [PATCH 060/359] publish fusioncatcher results

---
 modules/local/fusioncatcher/download/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index dbb3b8d5..c137d16c 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -19,8 +19,8 @@ process FUSIONCATCHER_DOWNLOAD {
     }
 
     output:
-    path "human_*"      , emit: reference
-    path "*.version.txt", emit: version
+    path "human_${human_version}/*"    , emit: reference
+    path "*.version.txt"               , emit: version
 
     script:
     def software = getSoftwareName(task.process)

From dc72942960a30f36c7cd46e865583c83d7be181b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 11:07:00 +0100
Subject: [PATCH 061/359] Change starfusion container and give absolute path to
 prep_genome_lib.pl

---
 modules/local/starfusion/download/main.nf | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 86044ef0..6a80b2bd 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -13,9 +13,9 @@ process STARFUSION_DOWNLOAD {
 
     conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/mulled-v2-57582e8bdbf51679bdcff9de91ae016a44e322de:b978a3a1e8715581329a267a2c9904574384180a-0"
+        container "docker.io/trinityctat/starfusion:1.10.1"
     } else {
-        container "quay.io/biocontainers/mulled-v2-57582e8bdbf51679bdcff9de91ae016a44e322de:b978a3a1e8715581329a267a2c9904574384180a-0"
+        container "docker.io/trinityctat/starfusion:1.10.1"
     }
 
     input:
@@ -38,10 +38,10 @@ process STARFUSION_DOWNLOAD {
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p
-    
+
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
 
-    prep_genome_lib.pl \\
+    /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --annot_filter_rule AnnotFilterRule.pm \\

From 4ba72f4991dfce2078cd42c152392fe653b45394 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 16:50:28 +0100
Subject: [PATCH 062/359] modify genome dir tree as only GRCh38

---
 conf/genomes.config | 22 +++++++++++-----------
 1 file changed, 11 insertions(+), 11 deletions(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index ec29a7c5..44de213d 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -2,7 +2,7 @@
  * -------------------------------------------------
  *  Nextflow config file for custom genomes
  * -------------------------------------------------
- * These references have to be build manually due 
+ * These references have to be build manually due
  * to ERCC spike-ins.
  * Can be used by any config that customizes the base
  * path using $params.genomes_base / --genomes_base
@@ -11,15 +11,15 @@
  params {
   genomes {
     'GRCh38' {
-      fasta         = "${params.genomes_base}/${params.genome}/Homo_sapiens.GRCh38.all.fa"
-      gtf           = "${params.genomes_base}/${params.genome}/Homo_sapiens.GRCh38.gtf"
-      transcript    = "${params.genomes_base}/${params.genome}/Homo_sapiens.GRCh38.cdna.all.fa.gz"
-      star          = "${params.genomes_base}/${params.genome}/STARIndex"
-      arriba        = "${params.genomes_base}/${params.genome}/arriba"
-      ericscript    = "${params.genomes_base}/${params.genome}/ericscript"
-      fusioncatcher = "${params.genomes_base}/${params.genome}/fusioncatcher"
-      pizzly        = "${params.genomes_base}/${params.genome}/pizzly"
-      starfusion    = "${params.genomes_base}/${params.genome}/starfusion"
+      fasta         = "${params.genomes_base}/Homo_sapiens.GRCh38.all.fa"
+      gtf           = "${params.genomes_base}/Homo_sapiens.GRCh38.chr.gtf"
+      transcript    = "${params.genomes_base}/Homo_sapiens.GRCh38.cdna.all.fa.gz"
+      star          = "${params.genomes_base}/star"
+      arriba        = "${params.genomes_base}/arriba"
+      ericscript    = "${params.genomes_base}/ericscript"
+      fusioncatcher = "${params.genomes_base}/fusioncatcher"
+      pizzly        = "${params.genomes_base}/pizzly"
+      starfusion    = "${params.genomes_base}/starfusion"
     }
   }
-}
\ No newline at end of file
+}

From db427fab876a2fd1bd7ff7d9009f0247c5001d1d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 16:53:01 +0100
Subject: [PATCH 063/359] --fasta is not required with --build_references

---
 lib/WorkflowRnafusion.groovy | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/lib/WorkflowRnafusion.groovy b/lib/WorkflowRnafusion.groovy
index e9d53d8a..f8997239 100755
--- a/lib/WorkflowRnafusion.groovy
+++ b/lib/WorkflowRnafusion.groovy
@@ -10,7 +10,7 @@ class WorkflowRnafusion {
     public static void initialise(params, log) {
         genomeExistsError(params, log)
 
-        if (!params.fasta) {
+        if (!params.fasta && !params.build_references) {
             log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
             System.exit(1)
         }

From 0f1b9a7ba18dc95d3228d57782720d4eb3a6ec0e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 17:00:03 +0100
Subject: [PATCH 064/359] only specify publish_dir in the modules main.nf for
 now

---
 conf/modules.config | 8 +-------
 1 file changed, 1 insertion(+), 7 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index d1674d9e..f5fceb08 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -20,6 +20,7 @@
 ----------------------------------------------------------------------------------------
 */
 
+
 params {
     modules {
         'fastqc' {
@@ -34,18 +35,15 @@ params {
         }
         'arriba_download' {
             args                = ""
-            publish_dir         = "references/${params.genome}/arriba"
         }
         'ensembl_download' {
             args                = ""
-            publish_dir         = "references/${params.genome}"
         }
         'ericscript' {
             args                = ""
         }
         'ericscript_download' {
             args                = ""
-            publish_dir         = "references/${params.genome}/ericscript"
         }
         'fusioncatcher' {
             args                = ""
@@ -53,18 +51,15 @@ params {
         'fusioncatcher_download' {
             args                = "--continue --tries=42"
             args2               = ""
-            publish_dir         = "references/${params.genome}/fusioncatcher"
         }
         'pizzly' {
             args                = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
         }
         'pizzly_download' {
             args                = "-k 31"
-            publish_dir         = "references/${params.genome}/pizzly"
         }
         'star_index' {
             args                = ""
-            publish_dir         = "references/${params.genome}/STARIndex"
         }
         'starfusion' {
             args                = "--chimSegmentMin 12 --chimJunctionOverhangMin 8 --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignIntronMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --outSAMattrRGline ID:GRPundef --chimMultimapScoreRange 3 --chimScoreJunctionNonGTAG -4 --chimMultimapNmax 20 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLminOverLmate 0 --alignSplicedMateMapLmin 30"
@@ -72,7 +67,6 @@ params {
         }
         'starfusion_download' {
             args                = ""
-            publish_dir         = "references/${params.genome}/starfusion"
         }
     }
 }

From 6c19f547212c3194417305428db93bee478d4dcb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 17:07:00 +0100
Subject: [PATCH 065/359] add build_reference param to main

---
 main.nf | 18 ++++++++++--------
 1 file changed, 10 insertions(+), 8 deletions(-)

diff --git a/main.nf b/main.nf
index c13e7b80..6648e59d 100644
--- a/main.nf
+++ b/main.nf
@@ -11,6 +11,13 @@
 
 nextflow.enable.dsl = 2
 
+/*
+========================================================================================
+    PARAMETER VALUES
+========================================================================================
+*/
+
+params.build_references           = WorkflowMain.getGenomeAttribute(params, 'build_references')
 /*
 ========================================================================================
     VALIDATE & PRINT PARAMETER SUMMARY
@@ -25,22 +32,17 @@ WorkflowMain.initialise(workflow, params, log)
 ========================================================================================
 */
 
+include { BUILD_REFERENCES } from './workflows/build_references'
+include { RNAFUSION } from './workflows/rnafusion'
 
 //
 // WORKFLOW: Run main nf-core/rnafusion analysis pipeline
 //
 workflow NFCORE_RNAFUSION {
-
     if (params.build_references) {
-
-        include { BUILD_REFERENCES } from './workflows/build_references'
         BUILD_REFERENCES ()
-    
     } else {
-
-        include { RNAFUSION } from './workflows/rnafusion'
-        // RNAFUSION ()
-
+        RNAFUSION ()
     }
 
 }

From 80b35869d468cedb95147fdf2b30fd7ea11057fc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 17:08:49 +0100
Subject: [PATCH 066/359] add build_reference param to nextflow.config

---
 nextflow.config | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/nextflow.config b/nextflow.config
index a87af0da..560c60c2 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -12,6 +12,9 @@ params {
     // Input options
     input                      = null
 
+    // Build reference or analyse
+    build_references           = false
+
     // MultiQC options
     multiqc_config             = null
     multiqc_title              = null

From e41c9e43016632aa645006d69a3eda7932246df9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 18:03:03 +0100
Subject: [PATCH 067/359] Change publish_dir to publishDir, remove function.nf
 module files and make modules dsl2 friendly

---
 conf/modules.config                           | 12 ++--
 docs/usage.md                                 |  4 +-
 modules/local/arriba/detect/functions.nf      | 68 -------------------
 modules/local/arriba/detect/main.nf           | 15 ++--
 modules/local/arriba/download/functions.nf    | 68 -------------------
 modules/local/arriba/download/main.nf         | 19 ++----
 modules/local/ensembl/functions.nf            | 68 -------------------
 modules/local/ensembl/main.nf                 | 17 ++---
 modules/local/ericscript/detect/functions.nf  | 68 -------------------
 modules/local/ericscript/detect/main.nf       | 19 ++----
 .../local/ericscript/download/functions.nf    | 68 -------------------
 modules/local/ericscript/download/main.nf     | 19 ++----
 modules/local/functions.nf                    | 68 -------------------
 .../local/fusioncatcher/detect/functions.nf   | 68 -------------------
 modules/local/fusioncatcher/detect/main.nf    | 15 ++--
 .../local/fusioncatcher/download/functions.nf | 68 -------------------
 modules/local/fusioncatcher/download/main.nf  | 17 ++---
 modules/local/pizzly/detect/functions.nf      | 68 -------------------
 modules/local/pizzly/detect/main.nf           | 19 ++----
 modules/local/pizzly/download/functions.nf    | 68 -------------------
 modules/local/pizzly/download/main.nf         | 19 ++----
 modules/local/samplesheet_check.nf            |  9 +--
 modules/local/star/align/functions.nf         | 68 -------------------
 modules/local/star/align/main.nf              | 15 ++--
 .../local/star/genomegenerate/functions.nf    | 68 -------------------
 modules/local/star/genomegenerate/main.nf     | 17 ++---
 modules/local/starfusion/detect/functions.nf  | 68 -------------------
 modules/local/starfusion/detect/main.nf       | 17 ++---
 .../local/starfusion/download/functions.nf    | 68 -------------------
 modules/local/starfusion/download/main.nf     | 13 +---
 modules/nf-core/modules/fastqc/functions.nf   | 68 -------------------
 modules/nf-core/modules/fastqc/main.nf        | 17 ++---
 .../modules/kallisto/index/functions.nf       | 68 -------------------
 .../nf-core/modules/kallisto/index/main.nf    | 15 ++--
 modules/nf-core/modules/multiqc/functions.nf  | 68 -------------------
 modules/nf-core/modules/multiqc/main.nf       | 15 ++--
 .../modules/samtools/faidx/functions.nf       | 68 -------------------
 .../nf-core/modules/samtools/faidx/main.nf    | 15 ++--
 nextflow.config                               |  4 +-
 nextflow_schema.json                          |  2 +-
 workflows/build_references.nf                 | 19 +++---
 41 files changed, 103 insertions(+), 1454 deletions(-)
 delete mode 100644 modules/local/arriba/detect/functions.nf
 delete mode 100644 modules/local/arriba/download/functions.nf
 delete mode 100644 modules/local/ensembl/functions.nf
 delete mode 100644 modules/local/ericscript/detect/functions.nf
 delete mode 100644 modules/local/ericscript/download/functions.nf
 delete mode 100644 modules/local/functions.nf
 delete mode 100644 modules/local/fusioncatcher/detect/functions.nf
 delete mode 100644 modules/local/fusioncatcher/download/functions.nf
 delete mode 100644 modules/local/pizzly/detect/functions.nf
 delete mode 100644 modules/local/pizzly/download/functions.nf
 delete mode 100644 modules/local/star/align/functions.nf
 delete mode 100644 modules/local/star/genomegenerate/functions.nf
 delete mode 100644 modules/local/starfusion/detect/functions.nf
 delete mode 100644 modules/local/starfusion/download/functions.nf
 delete mode 100644 modules/nf-core/modules/fastqc/functions.nf
 delete mode 100644 modules/nf-core/modules/kallisto/index/functions.nf
 delete mode 100644 modules/nf-core/modules/multiqc/functions.nf
 delete mode 100644 modules/nf-core/modules/samtools/faidx/functions.nf

diff --git a/conf/modules.config b/conf/modules.config
index f5fceb08..c818e04e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -6,14 +6,14 @@
         args            = Additional arguments appended to command in module.
         args2           = Second set of arguments appended to command in module (multi-tool modules).
         args3           = Third set of arguments appended to command in module (multi-tool modules).
-        publish_dir     = Directory to publish results.
-        publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path
-                            If publish_by_meta = true                 - Value of ${meta['id']} is appended as a directory to "publish_dir" path
+        publishDir      = Directory to publish results.
+        publish_by_meta = Groovy list of keys available in meta map to append as directories to "publishDir" path
+                            If publish_by_meta = true                 - Value of ${meta['id']} is appended as a directory to "publishDir" path
                             If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/"
-                                                                        is appended as a directory to "publish_dir" path
-                            If publish_by_meta = false / null         - No directories are appended to "publish_dir" path
+                                                                        is appended as a directory to "publishDir" path
+                            If publish_by_meta = false / null         - No directories are appended to "publishDir" path
         publish_files   = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension
-                            The value of "directory" is appended to the standard "publish_dir" path as defined above.
+                            The value of "directory" is appended to the standard "publishDir" path as defined above.
                             If publish_files = null (unspecified)     - All files are published.
                             If publish_files = false                  - No files are published.
         suffix          = File name suffix for output files.
diff --git a/docs/usage.md b/docs/usage.md
index f3dbed32..d334a977 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -204,7 +204,7 @@ Say for example we want to append an additional, non-mandatory parameter (i.e. `
 As you will see in the example below, we have:
 
 * appended `--outFilterMismatchNmax 16` to the default `args` used by the module.
-* changed the default `publish_dir` value to where the files will eventually be published in the main results directory.
+* changed the default `publishDir` value to where the files will eventually be published in the main results directory.
 * appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`.
 
 ```nextflow
@@ -212,7 +212,7 @@ params {
     modules {
         'star_align' {
             args          = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16"
-            publish_dir   = "my_star_directory"
+            publishDir    = "my_star_directory"
             publish_files = ['out':'log', 'tab':'log', 'bam':'']
         }
     }
diff --git a/modules/local/arriba/detect/functions.nf b/modules/local/arriba/detect/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/arriba/detect/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/arriba/detect/main.nf b/modules/local/arriba/detect/main.nf
index e9707d87..2d2c22ca 100644
--- a/modules/local/arriba/detect/main.nf
+++ b/modules/local/arriba/detect/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process ARRIBA {
     tag "$meta.id"
     label 'process_medium'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     conda (params.enable_conda ? "bioconda::arriba=2.1.0" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -27,10 +21,9 @@ process ARRIBA {
     output:
     tuple val(meta), path("*.fusions.tsv")          , emit: fusions
     tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail
-    path "*.version.txt"                            , emit: version
+    path "versions.yml"                             , emit: versions
 
     script:
-    def software  = getSoftwareName(task.process)
     def prefix    = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     STAR \\
@@ -49,6 +42,6 @@ process ARRIBA {
         -O ${prefix}.arriba.fusions.discarded.tsv \\
         $options.args2
 
-    echo \$(arriba -h | grep 'Version:' 2>&1) |  sed 's/Version:\s//' > ${software}.version.txt
+    echo \$(arriba -h | grep 'Version:' 2>&1) |  sed 's/Version:\s//' > versions.yml
     """
 }
diff --git a/modules/local/arriba/download/functions.nf b/modules/local/arriba/download/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/arriba/download/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/arriba/download/main.nf b/modules/local/arriba/download/main.nf
index 7cb029d2..05e96833 100644
--- a/modules/local/arriba/download/main.nf
+++ b/modules/local/arriba/download/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process ARRIBA_DOWNLOAD {
     tag "arriba"
     label 'process_low'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+    publishDir "${params.genomes_base}",
+        mode: params.publishDir_mode,
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -19,16 +13,15 @@ process ARRIBA_DOWNLOAD {
     }
 
     output:
-    path "*.version.txt"           , emit: version
+    path "*versions.yml"            , emit: versionss
     path "arriba_v2.1.0/database/*", emit: reference
 
     script:
-    def software = getSoftwareName(task.process)
     """
     wget https://github.com/suhrig/arriba/releases/download/v2.1.0/arriba_v2.1.0.tar.gz -O arriba_v2.1.0.tar.gz
     tar -xzvf arriba_v2.1.0.tar.gz
     rm arriba_v2.1.0.tar.gz
 
-    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
+    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """
-}
\ No newline at end of file
+}
diff --git a/modules/local/ensembl/functions.nf b/modules/local/ensembl/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/ensembl/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 08949eeb..8dd6d5b1 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process ENSEMBL_DOWNLOAD {
     tag "ensembl $ensembl_version"
     label 'process_low'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+    publishDir "${params.genomes_base}",
+        mode: params.publishDir_mode,
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -22,13 +16,12 @@ process ENSEMBL_DOWNLOAD {
     val ensembl_version
 
     output:
-    path "*.version.txt"                                                  , emit: version
+    path "versions.yml"                                                    , emit: versionss
     path "Homo_sapiens.${params.genome}.${ensembl_version}.all.fa"        , emit: fasta
     path "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"       , emit: gtf
     path "Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz", emit: transcript
 
     script:
-    def software = getSoftwareName(task.process)
     """
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
@@ -39,6 +32,6 @@ process ENSEMBL_DOWNLOAD {
     gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa
     gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
 
-    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
+    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """
 }
diff --git a/modules/local/ericscript/detect/functions.nf b/modules/local/ericscript/detect/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/ericscript/detect/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
index 94f6476d..4336f6d2 100644
--- a/modules/local/ericscript/detect/main.nf
+++ b/modules/local/ericscript/detect/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process ERICSCRIPT {
     tag "eriscript"
     label 'process_medium'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? "bioconda::ericscript=0.5.5" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -23,12 +17,11 @@ process ERICSCRIPT {
     path reference
 
     output:
-    path "*.version.txt"                                                   , emit: version
+    path "versions.yml"                                                    , emit: versions
     tuple val(meta), path "./tmp/${prefix}.ericscript.results.filtered.tsv", emit: fusions
     tuple val(meta), path "./tmp/${prefix}.ericscript.results.total.tsv"   , emit: fusions_total
-    
+
     script:
-    def software = getSoftwareName(task.process)
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     ericscript.pl \\
@@ -39,6 +32,6 @@ process ERICSCRIPT {
         $reads \\
         $options.args
 
-    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
+    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """
-}
\ No newline at end of file
+}
diff --git a/modules/local/ericscript/download/functions.nf b/modules/local/ericscript/download/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/ericscript/download/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/ericscript/download/main.nf b/modules/local/ericscript/download/main.nf
index 5ea73970..5441b6bc 100644
--- a/modules/local/ericscript/download/main.nf
+++ b/modules/local/ericscript/download/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process ERICSCRIPT_DOWNLOAD {
     tag "eriscript"
     label 'process_low'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+    publishDir "${params.genomes_base}",
+        mode: params.publishDir_mode,
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -19,16 +13,15 @@ process ERICSCRIPT_DOWNLOAD {
     }
 
     output:
-    path "*.version.txt" , emit: version
+    path "versions.yml"  , emit: versions
     path "homo_sapiens/*", emit: reference
 
     script:
-    def software = getSoftwareName(task.process)
     """
     wget http://ngi-igenomes.s3.amazonaws.com/igenomes/Homo_sapiens/Ensembl/GRCh38/Sequence/ericscript_db_homosapiens_ensembl84.tar.bz2
     tar jxf ericscript_db_homosapiens_ensembl84.tar.bz2 --strip-components=2
     rm ericscript_db_homosapiens_ensembl84.tar.bz2
 
-    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > ${software}.version.txt
+    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """
-}
\ No newline at end of file
+}
diff --git a/modules/local/functions.nf b/modules/local/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/fusioncatcher/detect/functions.nf b/modules/local/fusioncatcher/detect/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/fusioncatcher/detect/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 431d6a8f..a7731201 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process FUSIONCATCHER {
     tag "$meta.id"
     label 'process_high'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -26,10 +20,9 @@ process FUSIONCATCHER {
     tuple val(meta), path("*.fusioncatcher.fusion-genes.txt")   , optional:true, emit: fusions
     tuple val(meta), path("*.fusioncatcher.summary.txt")        , optional:true, emit: summary
     tuple val(meta), path("*.fusioncatcher.log")                               , emit: log
-    path "*.version.txt"                                                       , emit: version
+    path "versions.yml"                                                        , emit: versions
 
     script:
-    def software = getSoftwareName(task.process)
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     fusioncatcher.py \\
@@ -43,6 +36,6 @@ process FUSIONCATCHER {
     mv summary_candidate_fusions.txt ${prefix}.fusioncatcher.summary.txt
     mv fusioncatcher.log ${prefix}.fusioncatcher.log
 
-    fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
+    fusioncatcher --version | sed 's/fusioncatcher.py //' > versions.yml
     """
 }
diff --git a/modules/local/fusioncatcher/download/functions.nf b/modules/local/fusioncatcher/download/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/fusioncatcher/download/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index c137d16c..621f263b 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process FUSIONCATCHER_DOWNLOAD {
     tag 'fusioncatcher'
     label 'process_medium'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+    publishDir "${params.genomes_base}",
+        mode: params.publishDir_mode,
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -20,10 +14,9 @@ process FUSIONCATCHER_DOWNLOAD {
 
     output:
     path "human_${human_version}/*"    , emit: reference
-    path "*.version.txt"               , emit: version
+    path "versions.yml"                , emit: versions
 
     script:
-    def software = getSoftwareName(task.process)
     def human_version = "v102"
     def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.aa"
     """
@@ -41,6 +34,6 @@ process FUSIONCATCHER_DOWNLOAD {
             $options.args2
     fi
 
-    fusioncatcher --version | sed 's/fusioncatcher.py //' > ${software}.version.txt
+    fusioncatcher --version | sed 's/fusioncatcher.py //' > versions.yml
     """
 }
diff --git a/modules/local/pizzly/detect/functions.nf b/modules/local/pizzly/detect/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/pizzly/detect/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index b5fb67a8..71bdec18 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process PIZZLY {
     tag "pizzly"
     label 'process_medium'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2 bioconda::pizzly==0.37.3" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -27,12 +21,11 @@ process PIZZLY {
     path transcript
 
     output:
-    path "*.version.txt"                                    , emit: version
+    path "versions.yml"                                     , emit: versions
     tuple val(meta), path "${prefix}.pizzly.txt"            , emit: fusions
     tuple val(meta), path "${prefix}.pizzly.unfiltered.json", emit: fusions_unfiltered
-    
+
     script:
-    def software = getSoftwareName(task.process)
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     kallisto quant -t $task.cpus -i $index --fusion -o output $reads
@@ -44,6 +37,6 @@ process PIZZLY {
 
     pizzly_flatten_json.py ${prefix}.pizzly.json ${prefix}.pizzly.txt
 
-    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > ${software}.version.txt
+    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > versions.yml
     """
-}
\ No newline at end of file
+}
diff --git a/modules/local/pizzly/download/functions.nf b/modules/local/pizzly/download/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/pizzly/download/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/pizzly/download/main.nf b/modules/local/pizzly/download/main.nf
index a7468331..d3ba6b96 100644
--- a/modules/local/pizzly/download/main.nf
+++ b/modules/local/pizzly/download/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process PIZZLY_DOWNLOAD {
     tag "pizzly"
     label 'process_medium'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+    publishDir "${params.genomes_base}",
+        mode: params.publishDir_mode,
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -22,17 +16,16 @@ process PIZZLY_DOWNLOAD {
     path transcript
 
     output:
-    path "*.version.txt", emit: version
+    path "versions.yml" , emit: versions
     path "index.idx"    , emit: reference
 
     script:
-    def software = getSoftwareName(task.process)
     """
     kallisto index \\
         -i index.idx \\
         $options.args \\
         $transcript
 
-    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > ${software}.version.txt
+    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > versions.yml
     """
-}
\ No newline at end of file
+}
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index 15586d90..e73c31c2 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -1,14 +1,9 @@
-// Import generic module functions
-include { saveFiles } from './functions'
-
-params.options = [:]
-
 process SAMPLESHEET_CHECK {
     label 'process_low'
     tag "$samplesheet"
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:'pipeline_info', meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/star/align/functions.nf b/modules/local/star/align/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/star/align/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/star/align/main.nf b/modules/local/star/align/main.nf
index 82f1ca93..02f28cb7 100644
--- a/modules/local/star/align/main.nf
+++ b/modules/local/star/align/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process STAR_ALIGN {
     tag "$meta.id"
     label 'process_high'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     // Note: 2.7X indices incompatible with AWS iGenomes.
     conda (params.enable_conda ? 'bioconda::star=2.7.8a' : null)
@@ -29,7 +23,7 @@ process STAR_ALIGN {
     tuple val(meta), path('*Log.final.out')   , emit: log_final
     tuple val(meta), path('*Log.out')         , emit: log_out
     tuple val(meta), path('*Log.progress.out'), emit: log_progress
-    path  '*.version.txt'                     , emit: version
+    path  '*.version.txt'                      , emit: versions
 
     tuple val(meta), path('*sortedByCoord.out.bam')  , optional:true, emit: bam_sorted
     tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript
@@ -39,7 +33,6 @@ process STAR_ALIGN {
     tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
 
     script:
-    def software        = getSoftwareName(task.process)
     def prefix          = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     def ignore_gtf      = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
     def seq_platform    = params.seq_platform ? "'PL:$params.seq_platform'" : ""
@@ -68,6 +61,6 @@ process STAR_ALIGN {
         gzip ${prefix}.unmapped_2.fastq
     fi
 
-    STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt
+    STAR --version | sed -e "s/STAR_//g" > versions.yml
     """
 }
diff --git a/modules/local/star/genomegenerate/functions.nf b/modules/local/star/genomegenerate/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/star/genomegenerate/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/star/genomegenerate/main.nf b/modules/local/star/genomegenerate/main.nf
index 1eb4c0f4..dcf7e6e8 100644
--- a/modules/local/star/genomegenerate/main.nf
+++ b/modules/local/star/genomegenerate/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process STAR_GENOMEGENERATE {
     tag "$fasta"
     label 'process_high'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+    publishDir "${params.genomes_base}",
+        mode: params.publishDir_mode,
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -24,10 +18,9 @@ process STAR_GENOMEGENERATE {
 
     output:
     path "star/*"         , emit: index
-    path "*.version.txt"  , emit: version
+    path "versions.yml"   , emit: versionss
 
     script:
-    def software = getSoftwareName(task.process)
     def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
     def args     = options.args.tokenize()
     """
@@ -42,6 +35,6 @@ process STAR_GENOMEGENERATE {
         $memory \\
         $options.args
 
-    STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt
+    STAR --version | sed -e "s/STAR_//g" > versions.yml
     """
 }
diff --git a/modules/local/starfusion/detect/functions.nf b/modules/local/starfusion/detect/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/starfusion/detect/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index bf219417..edbbee0d 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process STARFUSION {
     tag "$meta.id"
     label 'process_high'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -26,10 +20,9 @@ process STARFUSION {
     tuple val(meta), path("*.fusion_predictions.tsv"), emit: fusions
     tuple val(meta), path("*.abridged.tsv")          , emit: abridged
     tuple val(meta), path("*.coding_effect.tsv")     , optional: true, emit: coding_effect
-    path "*.version.txt"                             , emit: version
+    path "versions.yml"                              , emit: versions
 
     script:
-    def software    = getSoftwareName(task.process)
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     STAR \\
@@ -48,7 +41,7 @@ process STARFUSION {
     mv star-fusion.fusion_predictions.tsv ${prefix}.starfusion.fusion_predictions.tsv
     mv star-fusion.fusion_predictions.abridged.tsv ${prefix}.starfusion.abridged.tsv
     mv star-fusion.fusion_predictions.abridged.coding_effect.tsv ${prefix}.starfusion.abridged.coding_effect.tsv
-    
-    echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > ${software}.version.txt
+
+    echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > versions.yml
     """
 }
diff --git a/modules/local/starfusion/download/functions.nf b/modules/local/starfusion/download/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/local/starfusion/download/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 6a80b2bd..c98ece5d 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
     label 'process_low'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+    publishDir "${params.genomes_base}",
+        mode: params.publishDir_mode,
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -26,7 +20,6 @@ process STARFUSION_DOWNLOAD {
     path "*"  , emit: reference
 
     script:
-    def software = getSoftwareName(task.process)
     """
     export TMPDIR=/tmp
 
diff --git a/modules/nf-core/modules/fastqc/functions.nf b/modules/nf-core/modules/fastqc/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/nf-core/modules/fastqc/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
index 39c327b2..4ef75822 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process FASTQC {
     tag "$meta.id"
     label 'process_medium'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -24,24 +18,23 @@ process FASTQC {
     output:
     tuple val(meta), path("*.html"), emit: html
     tuple val(meta), path("*.zip") , emit: zip
-    path  "*.version.txt"          , emit: version
+    path  "versions.yml"           , emit: versions
 
     script:
     // Add soft-links to original FastQs for consistent naming in pipeline
-    def software = getSoftwareName(task.process)
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     if (meta.single_end) {
         """
         [ ! -f  ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
         fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz
-        fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
+        fastqc --version | sed -e "s/FastQC v//g" > versions.yml
         """
     } else {
         """
         [ ! -f  ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
         [ ! -f  ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
         fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
-        fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
+        fastqc --version | sed -e "s/FastQC v//g" > versions.yml
         """
     }
 }
diff --git a/modules/nf-core/modules/kallisto/index/functions.nf b/modules/nf-core/modules/kallisto/index/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/nf-core/modules/kallisto/index/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/nf-core/modules/kallisto/index/main.nf b/modules/nf-core/modules/kallisto/index/main.nf
index 85eb7f0d..d0d155a5 100644
--- a/modules/nf-core/modules/kallisto/index/main.nf
+++ b/modules/nf-core/modules/kallisto/index/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process KALLISTO_INDEX {
     tag "$fasta"
     label 'process_medium'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -23,10 +17,9 @@ process KALLISTO_INDEX {
 
     output:
     path "kallisto" , emit: idx
-    path "*.version.txt", emit: version
+    path "versions.yml" , emit: versions
 
     script:
-    def software = getSoftwareName(task.process)
     """
     kallisto \\
         index \\
@@ -34,6 +27,6 @@ process KALLISTO_INDEX {
         -i kallisto \\
         $fasta
 
-    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > ${software}.version.txt
+    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > versions.yml
     """
 }
diff --git a/modules/nf-core/modules/multiqc/functions.nf b/modules/nf-core/modules/multiqc/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/nf-core/modules/multiqc/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index 8b6d6f0c..5fe6a83e 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -1,14 +1,8 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process MULTIQC {
     label 'process_medium'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -24,12 +18,11 @@ process MULTIQC {
     path "*multiqc_report.html", emit: report
     path "*_data"              , emit: data
     path "*_plots"             , optional:true, emit: plots
-    path "*.version.txt"       , emit: version
+    path "versions.yml"        , emit: versions
 
     script:
-    def software = getSoftwareName(task.process)
     """
     multiqc -f $options.args .
-    multiqc --version | sed -e "s/multiqc, version //g" > ${software}.version.txt
+    multiqc --version | sed -e "s/multiqc, version //g" > versions.yml
     """
 }
diff --git a/modules/nf-core/modules/samtools/faidx/functions.nf b/modules/nf-core/modules/samtools/faidx/functions.nf
deleted file mode 100644
index da9da093..00000000
--- a/modules/nf-core/modules/samtools/faidx/functions.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-//
-//  Utility functions used in nf-core DSL2 module files
-//
-
-//
-// Extract name of software tool from process name using $task.process
-//
-def getSoftwareName(task_process) {
-    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
-}
-
-//
-// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
-//
-def initOptions(Map args) {
-    def Map options = [:]
-    options.args            = args.args ?: ''
-    options.args2           = args.args2 ?: ''
-    options.args3           = args.args3 ?: ''
-    options.publish_by_meta = args.publish_by_meta ?: []
-    options.publish_dir     = args.publish_dir ?: ''
-    options.publish_files   = args.publish_files
-    options.suffix          = args.suffix ?: ''
-    return options
-}
-
-//
-// Tidy up and join elements of a list to return a path string
-//
-def getPathFromList(path_list) {
-    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
-    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
-    return paths.join('/')
-}
-
-//
-// Function to save/publish module results
-//
-def saveFiles(Map args) {
-    if (!args.filename.endsWith('.version.txt')) {
-        def ioptions  = initOptions(args.options)
-        def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
-        if (ioptions.publish_by_meta) {
-            def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
-            for (key in key_list) {
-                if (args.meta && key instanceof String) {
-                    def path = key
-                    if (args.meta.containsKey(key)) {
-                        path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
-                    }
-                    path = path instanceof String ? path : ''
-                    path_list.add(path)
-                }
-            }
-        }
-        if (ioptions.publish_files instanceof Map) {
-            for (ext in ioptions.publish_files) {
-                if (args.filename.endsWith(ext.key)) {
-                    def ext_list = path_list.collect()
-                    ext_list.add(ext.value)
-                    return "${getPathFromList(ext_list)}/$args.filename"
-                }
-            }
-        } else if (ioptions.publish_files == null) {
-            return "${getPathFromList(path_list)}/$args.filename"
-        }
-    }
-}
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf
index a89ff2bb..c87b4b68 100644
--- a/modules/nf-core/modules/samtools/faidx/main.nf
+++ b/modules/nf-core/modules/samtools/faidx/main.nf
@@ -1,15 +1,9 @@
-// Import generic module functions
-include { initOptions; saveFiles; getSoftwareName } from './functions'
-
-params.options = [:]
-options        = initOptions(params.options)
-
 process SAMTOOLS_FAIDX {
     tag "$fasta"
     label 'process_low'
     publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
+        mode: params.publishDir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? 'bioconda::samtools=1.13' : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -23,12 +17,11 @@ process SAMTOOLS_FAIDX {
 
     output:
     path "*.fai"        , emit: fai
-    path "*.version.txt", emit: version
+    path "versions.yml" , emit: versions
 
     script:
-    def software = getSoftwareName(task.process)
     """
     samtools faidx $fasta
-    echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt
+    echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > versions.yml
     """
 }
diff --git a/nextflow.config b/nextflow.config
index 560c60c2..37dcecb6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -11,7 +11,7 @@ params {
 
     // Input options
     input                      = null
-
+    fasta                      = null
     // Build reference or analyse
     build_references           = false
 
@@ -46,7 +46,7 @@ params {
     // Boilerplate options
     outdir                     = './results'
     tracedir                   = "${params.outdir}/pipeline_info"
-    publish_dir_mode           = 'copy'
+    publishDir_mode            = 'copy'
     email                      = null
     email_on_fail              = null
     plaintext_email            = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 13359495..f4640f94 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -163,7 +163,7 @@
                     "fa_icon": "fas fa-question-circle",
                     "hidden": true
                 },
-                "publish_dir_mode": {
+                "publishDir_mode": {
                     "type": "string",
                     "default": "copy",
                     "description": "Method used to save pipeline results to output directory.",
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 65fc812b..e29189a4 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -4,16 +4,13 @@
 ========================================================================================
 */
 
-// Don't overwrite global params.modules, create a copy instead and use that within the main script.
-def modules = params.modules.clone()
-
-include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'                    addParams( options: modules['ensembl_download'] )
-include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'            addParams( options: modules['arriba_download'] )
-include { STAR_GENOMEGENERATE }             from '../modules/local/star/genomegenerate/main'        addParams( options: modules['star_index'] )
-include { ERICSCRIPT_DOWNLOAD }             from '../modules/local/ericscript/download/main'        addParams( options: modules['ericscript_download'] )
-include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'     addParams( options: modules['fusioncatcher_download'] )
-include { KALLISTO_INDEX as PIZZLY_INDEX }  from '../modules/nf-core/modules/kallisto/index/main'   addParams( options: modules['pizzly_download'] )
-include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'        addParams( options: modules['starfusion_download'] )
+include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'
+include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'
+include { STAR_GENOMEGENERATE }             from '../modules/local/star/genomegenerate/main'
+include { ERICSCRIPT_DOWNLOAD }             from '../modules/local/ericscript/download/main'
+include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
+include { KALLISTO_INDEX as PIZZLY_INDEX }  from '../modules/nf-core/modules/kallisto/index/main'
+include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
 
 /*
 ========================================================================================
@@ -67,4 +64,4 @@ workflow.onComplete {
 ========================================================================================
     THE END
 ========================================================================================
-*/
\ No newline at end of file
+*/

From a4a4e096e4bf168ddd27a3e33dac78ed2fc740e3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 18:57:01 +0100
Subject: [PATCH 068/359] option.args -> args and ext.args in modules.config

---
 conf/modules.config                           | 40 +++++++++----------
 modules/local/arriba/detect/main.nf           |  6 ++-
 modules/local/ericscript/detect/main.nf       |  3 +-
 modules/local/fusioncatcher/detect/main.nf    |  3 +-
 modules/local/fusioncatcher/download/main.nf  | 13 +++---
 modules/local/pizzly/detect/main.nf           |  3 +-
 modules/local/pizzly/download/main.nf         |  3 +-
 modules/local/star/align/main.nf              |  7 ++--
 modules/local/star/genomegenerate/main.nf     |  4 +-
 modules/local/starfusion/detect/main.nf       |  6 ++-
 modules/nf-core/modules/fastqc/main.nf        | 11 ++---
 .../nf-core/modules/kallisto/index/main.nf    |  9 +++--
 modules/nf-core/modules/multiqc/main.nf       |  3 +-
 13 files changed, 63 insertions(+), 48 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index c818e04e..93f36471 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -3,9 +3,9 @@
     Config file for defining DSL2 per module options
 ========================================================================================
     Available keys to override module options:
-        args            = Additional arguments appended to command in module.
-        args2           = Second set of arguments appended to command in module (multi-tool modules).
-        args3           = Third set of arguments appended to command in module (multi-tool modules).
+        ext.args            = Additional arguments appended to command in module.
+        ext.args2           = Second set of arguments appended to command in module (multi-tool modules).
+        ext.args3           = Third set of arguments appended to command in module (multi-tool modules).
         publishDir      = Directory to publish results.
         publish_by_meta = Groovy list of keys available in meta map to append as directories to "publishDir" path
                             If publish_by_meta = true                 - Value of ${meta['id']} is appended as a directory to "publishDir" path
@@ -24,49 +24,49 @@
 params {
     modules {
         'fastqc' {
-            args                = "--quiet"
+            ext.args                = "--quiet"
         }
         'multiqc' {
-            args                = ""
+            ext.args                = ""
         }
         'arriba' {
-            args                = "--outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
-            args2               = "-b references/${params.genome}/arriba/blacklist_hg38_GRCh38_v2.1.0.tsv.gz -k references/${params.genome}/arriba/known_fusions_hg38_GRCh38_v2.1.0.tsv.gz -p references/${params.genome}/arriba/protein_domains_hg38_GRCh38_v2.1.0.gff3"
+            ext.args                = "--outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
+            ext.args2               = "-b references/${params.genome}/arriba/blacklist_hg38_GRCh38_v2.1.0.tsv.gz -k references/${params.genome}/arriba/known_fusions_hg38_GRCh38_v2.1.0.tsv.gz -p references/${params.genome}/arriba/protein_domains_hg38_GRCh38_v2.1.0.gff3"
         }
         'arriba_download' {
-            args                = ""
+            ext.args                = ""
         }
         'ensembl_download' {
-            args                = ""
+            ext.args                = ""
         }
         'ericscript' {
-            args                = ""
+            ext.args                = ""
         }
         'ericscript_download' {
-            args                = ""
+            ext.args                = ""
         }
         'fusioncatcher' {
-            args                = ""
+            ext.args                = ""
         }
         'fusioncatcher_download' {
-            args                = "--continue --tries=42"
-            args2               = ""
+            ext.args                = "--continue --tries=42"
+            ext.args2               = ""
         }
         'pizzly' {
-            args                = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
+            ext.args                = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
         }
         'pizzly_download' {
-            args                = "-k 31"
+            ext.args                = "-k 31"
         }
         'star_index' {
-            args                = ""
+            ext.args                = ""
         }
         'starfusion' {
-            args                = "--chimSegmentMin 12 --chimJunctionOverhangMin 8 --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignIntronMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --outSAMattrRGline ID:GRPundef --chimMultimapScoreRange 3 --chimScoreJunctionNonGTAG -4 --chimMultimapNmax 20 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLminOverLmate 0 --alignSplicedMateMapLmin 30"
-            args2               = "--examine_coding_effect -J Chimeric.out.junction"
+            ext.args                = "--chimSegmentMin 12 --chimJunctionOverhangMin 8 --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignIntronMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --outSAMattrRGline ID:GRPundef --chimMultimapScoreRange 3 --chimScoreJunctionNonGTAG -4 --chimMultimapNmax 20 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLminOverLmate 0 --alignSplicedMateMapLmin 30"
+            ext.args2               = "--examine_coding_effect -J Chimeric.out.junction"
         }
         'starfusion_download' {
-            args                = ""
+            ext.args                = ""
         }
     }
 }
diff --git a/modules/local/arriba/detect/main.nf b/modules/local/arriba/detect/main.nf
index 2d2c22ca..2a84ef27 100644
--- a/modules/local/arriba/detect/main.nf
+++ b/modules/local/arriba/detect/main.nf
@@ -25,12 +25,14 @@ process ARRIBA {
 
     script:
     def prefix    = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
     """
     STAR \\
 	    --genomeDir $index \\
 	    --readFilesIn $reads \\
 	    --runThreadN $task.cpus \\
-        $options.args |
+        $args |
 
     tee Aligned.out.bam |
 
@@ -40,7 +42,7 @@ process ARRIBA {
         -g $gtf \\
         -o ${prefix}.arriba.fusions.tsv \\
         -O ${prefix}.arriba.fusions.discarded.tsv \\
-        $options.args2
+        $args2
 
     echo \$(arriba -h | grep 'Version:' 2>&1) |  sed 's/Version:\s//' > versions.yml
     """
diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
index 4336f6d2..2b55726d 100644
--- a/modules/local/ericscript/detect/main.nf
+++ b/modules/local/ericscript/detect/main.nf
@@ -22,6 +22,7 @@ process ERICSCRIPT {
     tuple val(meta), path "./tmp/${prefix}.ericscript.results.total.tsv"   , emit: fusions_total
 
     script:
+    def args = task.ext.args ?: ''
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     ericscript.pl \\
@@ -30,7 +31,7 @@ process ERICSCRIPT {
         -p $task.cpus \\
         -o ./tmp \\
         $reads \\
-        $options.args
+        $args
 
     echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index a7731201..90712326 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -23,6 +23,7 @@ process FUSIONCATCHER {
     path "versions.yml"                                                        , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     fusioncatcher.py \\
@@ -30,7 +31,7 @@ process FUSIONCATCHER {
         -i $reads \\
         -p $task.cpus \\
         -o . \\
-        $options.args
+        $args
 
     mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher.fusion-genes.txt
     mv summary_candidate_fusions.txt ${prefix}.fusioncatcher.summary.txt
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 621f263b..a37b24c1 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -17,21 +17,24 @@ process FUSIONCATCHER_DOWNLOAD {
     path "versions.yml"                , emit: versions
 
     script:
+
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
     def human_version = "v102"
     def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.aa"
     """
     if wget --spider "$url" 2>/dev/null; then
-        wget $options.args $url
-        wget $options.args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ab
-        wget $options.args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ac
-        wget $options.args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ad
+        wget $args $url
+        wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ab
+        wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ac
+        wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ad
         cat human_${human_version}.tar.gz.* | tar xz
         rm human_${human_version}.tar*
     else
         fusioncatcher-build \\
             -g homo_sapiens \\
             -o human_${human_version} \\
-            $options.args2
+            $args2
     fi
 
     fusioncatcher --version | sed 's/fusioncatcher.py //' > versions.yml
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index 71bdec18..f3d4dbf5 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -26,11 +26,12 @@ process PIZZLY {
     tuple val(meta), path "${prefix}.pizzly.unfiltered.json", emit: fusions_unfiltered
 
     script:
+    def args = task.ext.args ?: ''
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     kallisto quant -t $task.cpus -i $index --fusion -o output $reads
     pizzly \\
-        $options.args \\
+        $args \\
         --gtf $gtf \\
         --fasta $transcript \\
         --output ${prefix}.pizzly output/fusion.txt
diff --git a/modules/local/pizzly/download/main.nf b/modules/local/pizzly/download/main.nf
index d3ba6b96..ded6da00 100644
--- a/modules/local/pizzly/download/main.nf
+++ b/modules/local/pizzly/download/main.nf
@@ -20,10 +20,11 @@ process PIZZLY_DOWNLOAD {
     path "index.idx"    , emit: reference
 
     script:
+    def args = task.ext.args ?: ''
     """
     kallisto index \\
         -i index.idx \\
-        $options.args \\
+        $args \\
         $transcript
 
     echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > versions.yml
diff --git a/modules/local/star/align/main.nf b/modules/local/star/align/main.nf
index 02f28cb7..d9556fab 100644
--- a/modules/local/star/align/main.nf
+++ b/modules/local/star/align/main.nf
@@ -33,12 +33,13 @@ process STAR_ALIGN {
     tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
 
     script:
+    def args = task.ext.args ?: ''
     def prefix          = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     def ignore_gtf      = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
     def seq_platform    = params.seq_platform ? "'PL:$params.seq_platform'" : ""
     def seq_center      = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform "
-    def out_sam_type    = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
-    def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
+    def out_sam_type    = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
+    def mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
     """
     STAR \\
         --genomeDir $index \\
@@ -48,7 +49,7 @@ process STAR_ALIGN {
         $out_sam_type \\
         $ignore_gtf \\
         $seq_center \\
-        $options.args
+        $args
 
     $mv_unsorted_bam
 
diff --git a/modules/local/star/genomegenerate/main.nf b/modules/local/star/genomegenerate/main.nf
index dcf7e6e8..13fecc96 100644
--- a/modules/local/star/genomegenerate/main.nf
+++ b/modules/local/star/genomegenerate/main.nf
@@ -22,7 +22,7 @@ process STAR_GENOMEGENERATE {
 
     script:
     def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
-    def args     = options.args.tokenize()
+    def args = task.ext.args ?: ''
     """
     mkdir star
     STAR \\
@@ -33,7 +33,7 @@ process STAR_GENOMEGENERATE {
         --sjdbOverhang ${params.read_length - 1} \\
         --runThreadN $task.cpus \\
         $memory \\
-        $options.args
+        $args
 
     STAR --version | sed -e "s/STAR_//g" > versions.yml
     """
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index edbbee0d..60725c8e 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -24,19 +24,21 @@ process STARFUSION {
 
     script:
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
     """
     STAR \\
 	    --genomeDir $index \\
 	    --readFilesIn $reads \\
 	    --runThreadN $task.cpus \\
-        $options.args
+        $args
 
     STAR-Fusion \\
         --genome_lib_dir $reference \\
         $reads \\
         --CPU $task.cpus \\
         --output_dir . \\
-        $options.args2
+        $args2
 
     mv star-fusion.fusion_predictions.tsv ${prefix}.starfusion.fusion_predictions.tsv
     mv star-fusion.fusion_predictions.abridged.tsv ${prefix}.starfusion.abridged.tsv
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
index 4ef75822..a39f889d 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -1,10 +1,10 @@
 process FASTQC {
     tag "$meta.id"
     label 'process_medium'
-    publishDir "${params.outdir}",
+    publishDir "${params.genomes_base}",
         mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
-
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        
     conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
         container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0"
@@ -21,19 +21,20 @@ process FASTQC {
     path  "versions.yml"           , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     // Add soft-links to original FastQs for consistent naming in pipeline
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     if (meta.single_end) {
         """
         [ ! -f  ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
-        fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz
+        fastqc $args --threads $task.cpus ${prefix}.fastq.gz
         fastqc --version | sed -e "s/FastQC v//g" > versions.yml
         """
     } else {
         """
         [ ! -f  ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
         [ ! -f  ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
-        fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
+        fastqc $args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
         fastqc --version | sed -e "s/FastQC v//g" > versions.yml
         """
     }
diff --git a/modules/nf-core/modules/kallisto/index/main.nf b/modules/nf-core/modules/kallisto/index/main.nf
index d0d155a5..7e14f3bd 100644
--- a/modules/nf-core/modules/kallisto/index/main.nf
+++ b/modules/nf-core/modules/kallisto/index/main.nf
@@ -1,10 +1,10 @@
 process KALLISTO_INDEX {
     tag "$fasta"
     label 'process_medium'
-    publishDir "${params.outdir}",
+    publishDir "${params.genomes_base}",
         mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
-
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
         container "https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1"
@@ -20,10 +20,11 @@ process KALLISTO_INDEX {
     path "versions.yml" , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     """
     kallisto \\
         index \\
-        $options.args \\
+        $args \\
         -i kallisto \\
         $fasta
 
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index 5fe6a83e..ed68dd8e 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -21,8 +21,9 @@ process MULTIQC {
     path "versions.yml"        , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     """
-    multiqc -f $options.args .
+    multiqc -f $args .
     multiqc --version | sed -e "s/multiqc, version //g" > versions.yml
     """
 }

From 47ea7e766925254111242d89aa46bbde4b71830c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 Dec 2021 19:02:55 +0100
Subject: [PATCH 069/359] comment out the rnasusion workflow for now

---
 main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/main.nf b/main.nf
index 6648e59d..5fb70dfa 100644
--- a/main.nf
+++ b/main.nf
@@ -42,7 +42,7 @@ workflow NFCORE_RNAFUSION {
     if (params.build_references) {
         BUILD_REFERENCES ()
     } else {
-        RNAFUSION ()
+        // RNAFUSION ()
     }
 
 }

From 880312c1c6186e0dc381c3141b30ec57a7cf430e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 23 Dec 2021 12:28:26 +0100
Subject: [PATCH 070/359] Increase cpu and time for high processes and set
 starfusion download as high + changed output for fusioncatcher

---
 conf/base.config                             | 4 ++--
 modules/local/fusioncatcher/download/main.nf | 4 ++--
 modules/local/starfusion/download/main.nf    | 2 +-
 3 files changed, 5 insertions(+), 5 deletions(-)

diff --git a/conf/base.config b/conf/base.config
index f4ce0af2..e36622cd 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -29,9 +29,9 @@ process {
         time   = { check_max( 8.h   * task.attempt, 'time'    ) }
     }
     withLabel:process_high {
-        cpus   = { check_max( 12    * task.attempt, 'cpus'    ) }
+        cpus   = { check_max( 20    * task.attempt, 'cpus'    ) }
         memory = { check_max( 72.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 16.h  * task.attempt, 'time'    ) }
+        time   = { check_max( 24.h  * task.attempt, 'time'    ) }
     }
     withLabel:process_long {
         time   = { check_max( 20.h  * task.attempt, 'time'    ) }
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index a37b24c1..56190c3a 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -13,8 +13,8 @@ process FUSIONCATCHER_DOWNLOAD {
     }
 
     output:
-    path "human_${human_version}/*"    , emit: reference
-    path "versions.yml"                , emit: versions
+    path "*"                , emit: reference
+    path "versions.yml"     , emit: versions
 
     script:
 
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index c98ece5d..47a6a7c8 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -1,6 +1,6 @@
 process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
-    label 'process_low'
+    label 'process_high'
     publishDir "${params.genomes_base}",
         mode: params.publishDir_mode,
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }

From 33184a3c1dab016033b595b40e6658e9cc3de07a Mon Sep 17 00:00:00 2001
From: Annick Renevey <annick.renevey@hasta.scilifelab.se>
Date: Tue, 11 Jan 2022 09:56:42 +0100
Subject: [PATCH 071/359] changed time and cpus allocated to starfusion don
 download and modification to fusioncatcher output

---
 conf/modules.config                          | 2 ++
 modules/local/fusioncatcher/download/main.nf | 4 ++--
 modules/local/starfusion/download/main.nf    | 2 +-
 3 files changed, 5 insertions(+), 3 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 93f36471..29e3f2d3 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -67,6 +67,8 @@ params {
         }
         'starfusion_download' {
             ext.args                = ""
+            cpus                    = 20
+            time                    = 120.h
         }
     }
 }
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index a37b24c1..56190c3a 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -13,8 +13,8 @@ process FUSIONCATCHER_DOWNLOAD {
     }
 
     output:
-    path "human_${human_version}/*"    , emit: reference
-    path "versions.yml"                , emit: versions
+    path "*"                , emit: reference
+    path "versions.yml"     , emit: versions
 
     script:
 
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index c98ece5d..47a6a7c8 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -1,6 +1,6 @@
 process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
-    label 'process_low'
+    label 'process_high'
     publishDir "${params.genomes_base}",
         mode: params.publishDir_mode,
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }

From 624ec946aef60dc8b4787fbb5f71095b274ffd97 Mon Sep 17 00:00:00 2001
From: Annick Renevey <annick.renevey@hasta.scilifelab.se>
Date: Tue, 11 Jan 2022 11:37:32 +0100
Subject: [PATCH 072/359] back to standard process_high + typo fix

---
 conf/base.config                      | 4 ++--
 modules/local/arriba/download/main.nf | 4 ++--
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/conf/base.config b/conf/base.config
index e36622cd..f4ce0af2 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -29,9 +29,9 @@ process {
         time   = { check_max( 8.h   * task.attempt, 'time'    ) }
     }
     withLabel:process_high {
-        cpus   = { check_max( 20    * task.attempt, 'cpus'    ) }
+        cpus   = { check_max( 12    * task.attempt, 'cpus'    ) }
         memory = { check_max( 72.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 24.h  * task.attempt, 'time'    ) }
+        time   = { check_max( 16.h  * task.attempt, 'time'    ) }
     }
     withLabel:process_long {
         time   = { check_max( 20.h  * task.attempt, 'time'    ) }
diff --git a/modules/local/arriba/download/main.nf b/modules/local/arriba/download/main.nf
index 05e96833..37d6c235 100644
--- a/modules/local/arriba/download/main.nf
+++ b/modules/local/arriba/download/main.nf
@@ -13,8 +13,8 @@ process ARRIBA_DOWNLOAD {
     }
 
     output:
-    path "*versions.yml"            , emit: versionss
-    path "arriba_v2.1.0/database/*", emit: reference
+    path "*versions.yml"            , emit: versions
+    path "arriba_v2.1.0/database/*" , emit: reference
 
     script:
     """

From d506476d7ab32d318e963ec860153ddc76bdf73e Mon Sep 17 00:00:00 2001
From: Annick Renevey <annick.renevey@hasta.scilifelab.se>
Date: Tue, 11 Jan 2022 11:44:48 +0100
Subject: [PATCH 073/359] small fixes

---
 modules/local/arriba/download/main.nf     | 2 +-
 modules/local/ensembl/main.nf             | 2 +-
 modules/local/star/align/main.nf          | 2 +-
 modules/local/star/genomegenerate/main.nf | 2 +-
 4 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/arriba/download/main.nf b/modules/local/arriba/download/main.nf
index 37d6c235..bfed085e 100644
--- a/modules/local/arriba/download/main.nf
+++ b/modules/local/arriba/download/main.nf
@@ -13,7 +13,7 @@ process ARRIBA_DOWNLOAD {
     }
 
     output:
-    path "*versions.yml"            , emit: versions
+    path "versions.yml"            , emit: versions
     path "arriba_v2.1.0/database/*" , emit: reference
 
     script:
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 8dd6d5b1..e9c7e5fa 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -16,7 +16,7 @@ process ENSEMBL_DOWNLOAD {
     val ensembl_version
 
     output:
-    path "versions.yml"                                                    , emit: versionss
+    path "versions.yml"                                                   , emit: versions
     path "Homo_sapiens.${params.genome}.${ensembl_version}.all.fa"        , emit: fasta
     path "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"       , emit: gtf
     path "Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz", emit: transcript
diff --git a/modules/local/star/align/main.nf b/modules/local/star/align/main.nf
index d9556fab..087414c3 100644
--- a/modules/local/star/align/main.nf
+++ b/modules/local/star/align/main.nf
@@ -23,7 +23,7 @@ process STAR_ALIGN {
     tuple val(meta), path('*Log.final.out')   , emit: log_final
     tuple val(meta), path('*Log.out')         , emit: log_out
     tuple val(meta), path('*Log.progress.out'), emit: log_progress
-    path  '*.version.txt'                      , emit: versions
+    path 'versions.yml'                       , emit: versions
 
     tuple val(meta), path('*sortedByCoord.out.bam')  , optional:true, emit: bam_sorted
     tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript
diff --git a/modules/local/star/genomegenerate/main.nf b/modules/local/star/genomegenerate/main.nf
index 13fecc96..d113fc20 100644
--- a/modules/local/star/genomegenerate/main.nf
+++ b/modules/local/star/genomegenerate/main.nf
@@ -18,7 +18,7 @@ process STAR_GENOMEGENERATE {
 
     output:
     path "star/*"         , emit: index
-    path "versions.yml"   , emit: versionss
+    path "versions.yml"   , emit: versions
 
     script:
     def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''

From 3fc794294ec513a0f87cc90decb09254f8e605b9 Mon Sep 17 00:00:00 2001
From: rannick <annick.renevey@hasta.scilifelab.se>
Date: Tue, 11 Jan 2022 13:48:29 +0100
Subject: [PATCH 074/359] Update fastqc module

---
 modules/nf-core/modules/fastqc/main.nf  | 27 ++++++++++++++-----------
 modules/nf-core/modules/fastqc/meta.yml |  7 ++++---
 2 files changed, 19 insertions(+), 15 deletions(-)

diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
index a39f889d..d250eca0 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -1,16 +1,11 @@
 process FASTQC {
     tag "$meta.id"
     label 'process_medium'
-    publishDir "${params.genomes_base}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        
+
     conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0"
-    } else {
-        container "quay.io/biocontainers/fastqc:0.11.9--0"
-    }
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' :
+        'quay.io/biocontainers/fastqc:0.11.9--0' }"
 
     input:
     tuple val(meta), path(reads)
@@ -23,19 +18,27 @@ process FASTQC {
     script:
     def args = task.ext.args ?: ''
     // Add soft-links to original FastQs for consistent naming in pipeline
-    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def prefix = task.ext.prefix ?: "${meta.id}"
     if (meta.single_end) {
         """
         [ ! -f  ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
         fastqc $args --threads $task.cpus ${prefix}.fastq.gz
-        fastqc --version | sed -e "s/FastQC v//g" > versions.yml
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        END_VERSIONS
         """
     } else {
         """
         [ ! -f  ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
         [ ! -f  ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
         fastqc $args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
-        fastqc --version | sed -e "s/FastQC v//g" > versions.yml
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        END_VERSIONS
         """
     }
 }
diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml
index 8eb9953d..b09553a3 100644
--- a/modules/nf-core/modules/fastqc/meta.yml
+++ b/modules/nf-core/modules/fastqc/meta.yml
@@ -15,6 +15,7 @@ tools:
             overrepresented sequences.
         homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
         documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
+        licence: ['GPL-2.0-only']
 input:
     - meta:
         type: map
@@ -40,10 +41,10 @@ output:
         type: file
         description: FastQC report archive
         pattern: "*_{fastqc.zip}"
-    - version:
+    - versions:
         type: file
-        description: File containing software version
-        pattern: "*.{version.txt}"
+        description: File containing software versions
+        pattern: "versions.yml"
 authors:
     - "@drpatelh"
     - "@grst"

From a7c3a7313c323a0d621db8751fadd61ab8eb3f42 Mon Sep 17 00:00:00 2001
From: rannick <annick.renevey@hasta.scilifelab.se>
Date: Tue, 11 Jan 2022 13:54:12 +0100
Subject: [PATCH 075/359] Update nf-core modules and nextflow_schema.json to
 include all arguments

---
 modules.json                                  |  8 +-
 .../nf-core/modules/kallisto/index/main.nf    | 18 ++---
 .../nf-core/modules/kallisto/index/meta.yml   | 12 +--
 modules/nf-core/modules/multiqc/main.nf       | 17 ++--
 modules/nf-core/modules/multiqc/meta.yml      |  7 +-
 .../nf-core/modules/samtools/faidx/main.nf    | 30 +++----
 .../nf-core/modules/samtools/faidx/meta.yml   | 17 +++-
 nextflow_schema.json                          | 78 +++++++++++++++++++
 8 files changed, 138 insertions(+), 49 deletions(-)

diff --git a/modules.json b/modules.json
index 7297e3d2..5c12d305 100644
--- a/modules.json
+++ b/modules.json
@@ -4,16 +4,16 @@
     "repos": {
         "nf-core/modules": {
             "fastqc": {
-                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+                "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961"
             },
             "kallisto/index": {
-                "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d"
+                "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41"
             },
             "multiqc": {
-                "git_sha": "c5235a983d454787fa0c3247b02086969217163b"
+                "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41"
             },
             "samtools/faidx": {
-                "git_sha": "c5235a983d454787fa0c3247b02086969217163b"
+                "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683"
             }
         }
     }
diff --git a/modules/nf-core/modules/kallisto/index/main.nf b/modules/nf-core/modules/kallisto/index/main.nf
index 7e14f3bd..4dc9c6d0 100644
--- a/modules/nf-core/modules/kallisto/index/main.nf
+++ b/modules/nf-core/modules/kallisto/index/main.nf
@@ -1,16 +1,11 @@
 process KALLISTO_INDEX {
     tag "$fasta"
     label 'process_medium'
-    publishDir "${params.genomes_base}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        
+
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1"
-    } else {
-        container "quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1"
-    }
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1' :
+        'quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1' }"
 
     input:
     path fasta
@@ -28,6 +23,9 @@ process KALLISTO_INDEX {
         -i kallisto \\
         $fasta
 
-    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        kallisto: \$(echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//')
+    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/modules/kallisto/index/meta.yml b/modules/nf-core/modules/kallisto/index/meta.yml
index 24b44b0b..dd952e33 100644
--- a/modules/nf-core/modules/kallisto/index/meta.yml
+++ b/modules/nf-core/modules/kallisto/index/meta.yml
@@ -1,7 +1,7 @@
 name: kallisto_index
-description: write your description here
+description: Create kallisto index
 keywords:
-  - sort
+  - index
 tools:
   - kallisto:
       description: Quantifying abundances of transcripts from bulk and single-cell RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads.
@@ -9,7 +9,7 @@ tools:
       documentation: https://pachterlab.github.io/kallisto/manual
       tool_dev_url: https://github.com/pachterlab/kallisto
       doi: ""
-      licence: ['BSD_2_clause']
+      licence: ['BSD-2-Clause']
 
 input:
   - fasta:
@@ -18,10 +18,10 @@ input:
       pattern: "*.{fasta}"
 
 output:
-  - version:
+  - versions:
       type: file
-      description: File containing software version
-      pattern: "*.{version.txt}"
+      description: File containing software versions
+      pattern: "versions.yml"
   - idx:
       type: index
       description: Kallisto genome index
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index ed68dd8e..3dceb162 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -1,15 +1,10 @@
 process MULTIQC {
     label 'process_medium'
-    publishDir "${params.outdir}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0"
-    } else {
-        container "quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0"
-    }
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }"
 
     input:
     path multiqc_files
@@ -24,6 +19,10 @@ process MULTIQC {
     def args = task.ext.args ?: ''
     """
     multiqc -f $args .
-    multiqc --version | sed -e "s/multiqc, version //g" > versions.yml
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
+    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml
index 532a8bb1..63c75a45 100644
--- a/modules/nf-core/modules/multiqc/meta.yml
+++ b/modules/nf-core/modules/multiqc/meta.yml
@@ -11,6 +11,7 @@ tools:
             It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
         homepage: https://multiqc.info/
         documentation: https://multiqc.info/docs/
+        licence: ['GPL-3.0-or-later']
 input:
     - multiqc_files:
         type: file
@@ -29,10 +30,10 @@ output:
         type: file
         description: Plots created by MultiQC
         pattern: "*_data"
-    - version:
+    - versions:
         type: file
-        description: File containing software version
-        pattern: "*.{version.txt}"
+        description: File containing software versions
+        pattern: "versions.yml"
 authors:
     - "@abhi18av"
     - "@bunop"
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf
index c87b4b68..58c445d8 100644
--- a/modules/nf-core/modules/samtools/faidx/main.nf
+++ b/modules/nf-core/modules/samtools/faidx/main.nf
@@ -1,27 +1,29 @@
 process SAMTOOLS_FAIDX {
     tag "$fasta"
     label 'process_low'
-    publishDir "${params.outdir}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
-    conda (params.enable_conda ? 'bioconda::samtools=1.13' : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0"
-    } else {
-        container "quay.io/biocontainers/samtools:1.13--h8c37831_0"
-    }
+    conda (params.enable_conda ? "bioconda::samtools=1.14" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' :
+        'quay.io/biocontainers/samtools:1.14--hb421002_0' }"
 
     input:
-    path fasta
+    tuple val(meta), path(fasta)
 
     output:
-    path "*.fai"        , emit: fai
-    path "versions.yml" , emit: versions
+    tuple val(meta), path ("*.fai"), emit: fai
+    path "versions.yml"            , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     """
-    samtools faidx $fasta
-    echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > versions.yml
+    samtools \\
+        faidx \\
+        $fasta
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/modules/samtools/faidx/meta.yml b/modules/nf-core/modules/samtools/faidx/meta.yml
index f92234d0..bae97a39 100644
--- a/modules/nf-core/modules/samtools/faidx/meta.yml
+++ b/modules/nf-core/modules/samtools/faidx/meta.yml
@@ -12,20 +12,31 @@ tools:
         homepage: http://www.htslib.org/
         documentation: http://www.htslib.org/doc/samtools.html
         doi: 10.1093/bioinformatics/btp352
+        licence: ['MIT']
 input:
+    - meta:
+        type: map
+        description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
     - fasta:
         type: file
         description: FASTA file
         pattern: "*.{fa,fasta}"
 output:
+    - meta:
+        type: map
+        description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
     - fai:
         type: file
         description: FASTA index file
         pattern: "*.{fai}"
-    - version:
+    - versions:
         type: file
-        description: File containing software version
-        pattern: "*.{version.txt}"
+        description: File containing software versions
+        pattern: "versions.yml"
 authors:
     - "@drpatelh"
     - "@ewels"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index f4640f94..34c39e4e 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -41,6 +41,84 @@
                     "type": "string",
                     "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.",
                     "fa_icon": "fas fa-file-signature"
+                },
+                "build_references": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Specifies which analysis type for the pipeline - either build references or analyse data",
+                    "default": "false"
+                },
+                "genome": {
+                    "type": "string",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Which genome version",
+                    "default": "GRCh38"
+                },
+                "genomes_base": {
+                    "type": "string",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Path to reference folder",
+                    "default": "GRCh38"
+                },
+                "ensembl_version": {
+                    "type": "string",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "ensembl version",
+                    "default": "97"
+                },
+                "read_length": {
+                    "type": "string",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Read length",
+                    "default": "100"
+                },
+                "all": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Build or run all references/analyses",
+                    "default": "true"
+                },
+                "arriba": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Build or run arriba references/analyses",
+                    "default": "true"
+                },
+                "ericscript": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Build or run ericscript references/analyses",
+                    "default": "true"
+                },
+                "fusioncatcher": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Build or run fusioncatcher references/analyses",
+                    "default": "true"
+                },
+                "pizzly": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Build or run pizzly references/analyses",
+                    "default": "true"
+                },
+                "squid": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Build or run squid references/analyses",
+                    "default": "true"
+                },
+                "starindex": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Build or run starindex references/analyses",
+                    "default": "true"
+                },
+                "starfusion": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-user-cog",
+                    "description": "Build or run starfusion references/analyses",
+                    "default": "true"
                 }
             }
         },

From 5bcdc4b2656a12b549354d06d236709774522062 Mon Sep 17 00:00:00 2001
From: rannick <annick.renevey@hasta.scilifelab.se>
Date: Tue, 11 Jan 2022 14:00:00 +0100
Subject: [PATCH 076/359] fix files_unchanged

---
 .gitattributes             |  2 ++
 assets/multiqc_config.yaml |  2 +-
 lib/NfcoreSchema.groovy    |  3 +--
 lib/NfcoreTemplate.groovy  | 30 +++++++++---------------------
 4 files changed, 13 insertions(+), 24 deletions(-)

diff --git a/.gitattributes b/.gitattributes
index 7fe55006..050bb120 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -1 +1,3 @@
 *.config linguist-language=nextflow
+modules/nf-core/** linguist-generated
+subworkflows/nf-core/** linguist-generated
diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml
index 14f8ada3..611287cc 100644
--- a/assets/multiqc_config.yaml
+++ b/assets/multiqc_config.yaml
@@ -1,7 +1,7 @@
 report_comment: >
     This report has been generated by the <a href="https://github.com/nf-core/rnafusion" target="_blank">nf-core/rnafusion</a>
     analysis pipeline. For information about how to interpret these results, please see the
-    <a href="https://github.com/nf-core/rnafusion" target="_blank">documentation</a>.
+    <a href="https://nf-co.re/rnafusion" target="_blank">documentation</a>.
 report_section_order:
     software_versions:
         order: -1000
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index 8d6920dd..dcb39c83 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -260,13 +260,12 @@ class NfcoreSchema {
 
         // Get pipeline parameters defined in JSON Schema
         def Map params_summary = [:]
-        def blacklist  = ['hostnames']
         def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename))
         for (group in params_map.keySet()) {
             def sub_params = new LinkedHashMap()
             def group_params = params_map.get(group)  // This gets the parameters of that particular group
             for (param in group_params.keySet()) {
-                if (params.containsKey(param) && !blacklist.contains(param)) {
+                if (params.containsKey(param)) {
                     def params_value = params.get(param)
                     def schema_value = group_params.get(param).default
                     def param_type   = group_params.get(param).type
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index 44551e0a..06499409 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -19,27 +19,16 @@ class NfcoreTemplate {
     }
 
     //
-    // Check params.hostnames
+    //  Warn if a -profile or Nextflow config has not been provided to run the pipeline
     //
-    public static void hostName(workflow, params, log) {
-        Map colors = logColours(params.monochrome_logs)
-        if (params.hostnames) {
-            try {
-                def hostname = "hostname".execute().text.trim()
-                params.hostnames.each { prof, hnames ->
-                    hnames.each { hname ->
-                        if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                            log.info "=${colors.yellow}====================================================${colors.reset}=\n" +
-                                "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" +
-                                "      but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" +
-                                "      ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" +
-                                "=${colors.yellow}====================================================${colors.reset}="
-                        }
-                    }
-                }
-            } catch (Exception e) {
-                log.warn "[$workflow.manifest.name] Could not determine 'hostname' - skipping check. Reason: ${e.message}."
-            }
+    public static void checkConfigProvided(workflow, log) {
+        if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
+            log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
+                    "This will be dependent on your local compute enviroment but can be acheived via one or more of the following:\n" +
+                    "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
+                    "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
+                    "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
+                    "Please refer to the quick start section and usage docs for the pipeline.\n "
         }
     }
 
@@ -168,7 +157,6 @@ class NfcoreTemplate {
                 log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
             }
         } else {
-            hostName(workflow, params, log)
             log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
         }
     }

From f44d98d3d7286613faccac0f9ed0f8d7d395eabf Mon Sep 17 00:00:00 2001
From: rannick <annick.renevey@hasta.scilifelab.se>
Date: Tue, 11 Jan 2022 14:12:06 +0100
Subject: [PATCH 077/359] fic padding

---
 conf/genomes.config                     | 26 ++++++++++++-------------
 modules.json                            |  2 +-
 modules/local/arriba/detect/main.nf     |  6 +++---
 modules/local/starfusion/detect/main.nf |  6 +++---
 4 files changed, 20 insertions(+), 20 deletions(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index 44de213d..869bf065 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -8,18 +8,18 @@
  * path using $params.genomes_base / --genomes_base
  */
 
- params {
-  genomes {
-    'GRCh38' {
-      fasta         = "${params.genomes_base}/Homo_sapiens.GRCh38.all.fa"
-      gtf           = "${params.genomes_base}/Homo_sapiens.GRCh38.chr.gtf"
-      transcript    = "${params.genomes_base}/Homo_sapiens.GRCh38.cdna.all.fa.gz"
-      star          = "${params.genomes_base}/star"
-      arriba        = "${params.genomes_base}/arriba"
-      ericscript    = "${params.genomes_base}/ericscript"
-      fusioncatcher = "${params.genomes_base}/fusioncatcher"
-      pizzly        = "${params.genomes_base}/pizzly"
-      starfusion    = "${params.genomes_base}/starfusion"
+params {
+    genomes {
+        'GRCh38' {
+            fasta         = "${params.genomes_base}/Homo_sapiens.GRCh38.all.fa"
+            gtf           = "${params.genomes_base}/Homo_sapiens.GRCh38.chr.gtf"
+            transcript    = "${params.genomes_base}/Homo_sapiens.GRCh38.cdna.all.fa.gz"
+            star          = "${params.genomes_base}/star"
+            arriba        = "${params.genomes_base}/arriba"
+            ericscript    = "${params.genomes_base}/ericscript"
+            fusioncatcher = "${params.genomes_base}/fusioncatcher"
+            pizzly        = "${params.genomes_base}/pizzly"
+            starfusion    = "${params.genomes_base}/starfusion"
+        }
     }
-  }
 }
diff --git a/modules.json b/modules.json
index 5c12d305..67b55c50 100644
--- a/modules.json
+++ b/modules.json
@@ -17,4 +17,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/local/arriba/detect/main.nf b/modules/local/arriba/detect/main.nf
index 2a84ef27..c3460fba 100644
--- a/modules/local/arriba/detect/main.nf
+++ b/modules/local/arriba/detect/main.nf
@@ -29,9 +29,9 @@ process ARRIBA {
     def args2 = task.ext.args2 ?: ''
     """
     STAR \\
-	    --genomeDir $index \\
-	    --readFilesIn $reads \\
-	    --runThreadN $task.cpus \\
+        --genomeDir $index \\
+        --readFilesIn $reads \\
+        --runThreadN $task.cpus \\
         $args |
 
     tee Aligned.out.bam |
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 60725c8e..1b6c1afb 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -28,9 +28,9 @@ process STARFUSION {
     def args2 = task.ext.args2 ?: ''
     """
     STAR \\
-	    --genomeDir $index \\
-	    --readFilesIn $reads \\
-	    --runThreadN $task.cpus \\
+        --genomeDir $index \\
+        --readFilesIn $reads \\
+        --runThreadN $task.cpus \\
         $args
 
     STAR-Fusion \\

From 41870e319553fdb55d65ae8f7397df17fe005fd3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 11 Jan 2022 14:54:23 +0100
Subject: [PATCH 078/359] generate dark logo, change issue templates

---
 .github/CONTRIBUTING.md                      |  38 +++--------
 .github/ISSUE_TEMPLATE/bug_report.md         |  63 -------------------
 .github/ISSUE_TEMPLATE/bug_report.yml        |  52 +++++++++++++++
 .github/ISSUE_TEMPLATE/config.yml            |   1 -
 .github/ISSUE_TEMPLATE/feature_request.md    |  32 ----------
 .github/ISSUE_TEMPLATE/feature_request.yml   |  11 ++++
 .github/workflows/linting_comment.yml        |   1 +
 assets/sendmail_template.txt                 |   4 +-
 docs/images/nf-core-rnafusion_logo.png       | Bin 35022 -> 0 bytes
 docs/images/nf-core-rnafusion_logo_dark.png  | Bin 0 -> 72761 bytes
 docs/images/nf-core-rnafusion_logo_light.png | Bin 0 -> 72629 bytes
 lib/NfcoreSchema.groovy                      |  27 +++++---
 lib/NfcoreTemplate.groovy                    |   2 +-
 13 files changed, 94 insertions(+), 137 deletions(-)
 delete mode 100644 .github/ISSUE_TEMPLATE/bug_report.md
 create mode 100644 .github/ISSUE_TEMPLATE/bug_report.yml
 delete mode 100644 .github/ISSUE_TEMPLATE/feature_request.md
 create mode 100644 .github/ISSUE_TEMPLATE/feature_request.yml
 delete mode 100644 docs/images/nf-core-rnafusion_logo.png
 create mode 100644 docs/images/nf-core-rnafusion_logo_dark.png
 create mode 100644 docs/images/nf-core-rnafusion_logo_light.png

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 1912ed9d..4dfbe44c 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -68,16 +68,13 @@ If you wish to contribute a new step, please use the following coding standards:
 1. Define the corresponding input channel into your new process from the expected previous process channel
 2. Write the process block (see below).
 3. Define the output channel if needed (see below).
-4. Add any new flags/options to `nextflow.config` with a default (see below).
-5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build`).
-6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter).
-7. Add sanity checks for all relevant parameters.
-8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`.
-9. Do local tests that the new code works properly and as expected.
-10. Add a new test command in `.github/workflow/ci.yml`.
-11. If applicable add a [MultiQC](https://https://multiqc.info/) module.
-12. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, name clean up, General Statistics Table column order, and module figures are in the right order.
-13. Optional: Add any descriptions of MultiQC report sections and output files to `docs/output.md`.
+4. Add any new parameters to `nextflow.config` with a default (see below).
+5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core schema build` tool).
+6. Add sanity checks and validation for all relevant parameters.
+7. Perform local tests to validate that the new code works as expected.
+8. If applicable, add a new test command in `.github/workflow/ci.yml`.
+9. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module.
+10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`.
 
 ### Default values
 
@@ -102,27 +99,6 @@ Please use the following naming schemes, to make it easy to understand what is g
 
 If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]`
 
-### Software version reporting
-
-If you add a new tool to the pipeline, please ensure you add the information of the tool to the `get_software_version` process.
-
-Add to the script block of the process, something like the following:
-
-```bash
-<YOUR_TOOL> --version &> v_<YOUR_TOOL>.txt 2>&1 || true
-```
-
-or
-
-```bash
-<YOUR_TOOL> --help | head -n 1 &> v_<YOUR_TOOL>.txt 2>&1 || true
-```
-
-You then need to edit the script `bin/scrape_software_versions.py` to:
-
-1. Add a Python regex for your tool's `--version` output (as in stored in the `v_<YOUR_TOOL>.txt` file), to ensure the version is reported as a `v` and the version number e.g. `v2.1.1`
-2. Add a HTML entry to the `OrderedDict` for formatting in MultiQC.
-
 ### Images and figures
 
 For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines).
diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md
deleted file mode 100644
index 21b44f28..00000000
--- a/.github/ISSUE_TEMPLATE/bug_report.md
+++ /dev/null
@@ -1,63 +0,0 @@
----
-name: Bug report
-about: Report something that is broken or incorrect
-labels: bug
----
-
-<!--
-# nf-core/rnafusion bug report
-
-Hi there!
-
-Thanks for telling us about a problem with the pipeline.
-Please delete this text and anything that's not relevant from the template below:
--->
-
-## Check Documentation
-
-I have checked the following places for your error:
-
-- [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
-- [ ] [nf-core/rnafusion pipeline documentation](https://nf-co.re/rnafusion/usage)
-
-## Description of the bug
-
-<!-- A clear and concise description of what the bug is. -->
-
-## Steps to reproduce
-
-Steps to reproduce the behaviour:
-
-1. Command line: <!-- [e.g. `nextflow run ...`] -->
-2. See error: <!-- [Please provide your error message] -->
-
-## Expected behaviour
-
-<!-- A clear and concise description of what you expected to happen. -->
-
-## Log files
-
-Have you provided the following extra information/files:
-
-- [ ] The command used to run the pipeline
-- [ ] The `.nextflow.log` file <!-- this is a hidden file in the directory where you launched the pipeline -->
-
-## System
-
-- Hardware: <!-- [e.g. HPC, Desktop, Cloud...] -->
-- Executor: <!-- [e.g. slurm, local, awsbatch...] -->
-- OS: <!-- [e.g. CentOS Linux, macOS, Linux Mint...] -->
-- Version <!-- [e.g. 7, 10.13.6, 18.3...] -->
-
-## Nextflow Installation
-
-- Version: <!-- [e.g. 21.04.0] -->
-
-## Container engine
-
-- Engine: <!-- [e.g. Conda, Docker, Singularity, Podman, Shifter or Charliecloud] -->
-- version: <!-- [e.g. 1.0.0] -->
-
-## Additional context
-
-<!-- Add any other context about the problem here. -->
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
new file mode 100644
index 00000000..e904de57
--- /dev/null
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -0,0 +1,52 @@
+
+name: Bug report
+description: Report something that is broken or incorrect
+labels: bug
+body:
+
+  - type: markdown
+    attributes:
+      value: |
+        Before you post this issue, please check the documentation:
+
+        - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
+        - [{{ name }} pipeline documentation](https://nf-co.re/{{ short_name }}/usage)
+
+  - type: textarea
+    id: description
+    attributes:
+      label: Description of the bug
+      description: A clear and concise description of what the bug is.
+    validations:
+      required: true
+
+  - type: textarea
+    id: command_used
+    attributes:
+      label: Command used and terminal output
+      description: Steps to reproduce the behaviour. Please paste the command you used to launch the pipeline and the output from your terminal.
+      render: console
+      placeholder: |
+        $ nextflow run ...
+
+        Some output where something broke
+
+  - type: textarea
+    id: files
+    attributes:
+      label: Relevant files
+      description: |
+        Please drag and drop the relevant files here. Create a `.zip` archive if the extension is not allowed.
+        Your verbose log file `.nextflow.log` is often useful _(this is a hidden file in the directory where you launched the pipeline)_ as well as custom Nextflow configuration files.
+
+  - type: textarea
+    id: system
+    attributes:
+      label: System information
+      description: |
+        * Nextflow version _(eg. 21.10.3)_
+        * Hardware _(eg. HPC, Desktop, Cloud)_
+        * Executor _(eg. slurm, local, awsbatch)_
+        * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_
+        * OS _(eg. CentOS Linux, macOS, Linux Mint)_
+        * Version of {{ name }} _(eg. 1.1, 1.5, 1.8.2)_
diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml
index 1fccc741..69a065e7 100644
--- a/.github/ISSUE_TEMPLATE/config.yml
+++ b/.github/ISSUE_TEMPLATE/config.yml
@@ -1,4 +1,3 @@
-blank_issues_enabled: false
 contact_links:
   - name: Join nf-core
     url: https://nf-co.re/join
diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md
deleted file mode 100644
index 10da2c83..00000000
--- a/.github/ISSUE_TEMPLATE/feature_request.md
+++ /dev/null
@@ -1,32 +0,0 @@
----
-name: Feature request
-about: Suggest an idea for the nf-core/rnafusion pipeline
-labels: enhancement
----
-
-<!--
-# nf-core/rnafusion feature request
-
-Hi there!
-
-Thanks for suggesting a new feature for the pipeline!
-Please delete this text and anything that's not relevant from the template below:
--->
-
-## Is your feature request related to a problem? Please describe
-
-<!-- A clear and concise description of what the problem is. -->
-
-<!-- e.g. [I'm always frustrated when ...] -->
-
-## Describe the solution you'd like
-
-<!-- A clear and concise description of what you want to happen. -->
-
-## Describe alternatives you've considered
-
-<!-- A clear and concise description of any alternative solutions or features you've considered. -->
-
-## Additional context
-
-<!-- Add any other context about the feature request here. -->
diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml
new file mode 100644
index 00000000..ad3a715d
--- /dev/null
+++ b/.github/ISSUE_TEMPLATE/feature_request.yml
@@ -0,0 +1,11 @@
+name: Feature request
+description: Suggest an idea for the {{ name }} pipeline
+labels: enhancement
+body:
+  - type: textarea
+    id: description
+    attributes:
+      label: Description of feature
+      description: Please describe your suggestion for a new feature. It might help to describe a problem or use case, plus any alternatives that you have considered.
+    validations:
+      required: true
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 90f03c6f..44d72994 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -15,6 +15,7 @@ jobs:
         uses: dawidd6/action-download-artifact@v2
         with:
           workflow: linting.yml
+          workflow_conclusion: completed
 
       - name: Get PR number
         id: pr_number
diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt
index 06728832..ff6631ad 100644
--- a/assets/sendmail_template.txt
+++ b/assets/sendmail_template.txt
@@ -12,9 +12,9 @@ $email_html
 Content-Type: image/png;name="nf-core-rnafusion_logo.png"
 Content-Transfer-Encoding: base64
 Content-ID: <nfcorepipelinelogo>
-Content-Disposition: inline; filename="nf-core-rnafusion_logo.png"
+Content-Disposition: inline; filename="nf-core-rnafusion_logo_light.png"
 
-<% out << new File("$projectDir/assets/nf-core-rnafusion_logo.png").
+<% out << new File("$projectDir/assets/nf-core-rnafusion_logo_light.png").
     bytes.
     encodeBase64().
     toString().
diff --git a/docs/images/nf-core-rnafusion_logo.png b/docs/images/nf-core-rnafusion_logo.png
deleted file mode 100644
index 97835e0eb03020a770579e936da155e9fddb0f90..0000000000000000000000000000000000000000
GIT binary patch
literal 0
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diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index dcb39c83..b3d092f8 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -27,7 +27,7 @@ class NfcoreSchema {
     /* groovylint-disable-next-line UnusedPrivateMethodParameter */
     public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') {
         def has_error = false
-        //=====================================================================//
+        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
         // Check for nextflow core params and unexpected params
         def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text
         def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
@@ -105,9 +105,13 @@ class NfcoreSchema {
 
         // Collect expected parameters from the schema
         def expectedParams = []
+        def enums = [:]
         for (group in schemaParams) {
             for (p in group.value['properties']) {
                 expectedParams.push(p.key)
+                if (group.value['properties'][p.key].containsKey('enum')) {
+                    enums[p.key] = group.value['properties'][p.key]['enum']
+                }
             }
         }
 
@@ -131,7 +135,7 @@ class NfcoreSchema {
             }
         }
 
-        //=====================================================================//
+        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
         // Validate parameters against the schema
         InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream()
         JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream))
@@ -155,7 +159,7 @@ class NfcoreSchema {
             println ''
             log.error 'ERROR: Validation of pipeline parameters failed!'
             JSONObject exceptionJSON = e.toJSON()
-            printExceptions(exceptionJSON, params_json, log)
+            printExceptions(exceptionJSON, params_json, log, enums)
             println ''
             has_error = true
         }
@@ -202,7 +206,7 @@ class NfcoreSchema {
                 }
                 def type = '[' + group_params.get(param).type + ']'
                 def description = group_params.get(param).description
-                def defaultValue = group_params.get(param).default ? " [default: " + group_params.get(param).default.toString() + "]" : ''
+                def defaultValue = group_params.get(param).default != null ? " [default: " + group_params.get(param).default.toString() + "]" : ''
                 def description_default = description + colors.dim + defaultValue + colors.reset
                 // Wrap long description texts
                 // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap
@@ -329,7 +333,7 @@ class NfcoreSchema {
     //
     // Loop over nested exceptions and print the causingException
     //
-    private static void printExceptions(ex_json, params_json, log) {
+    private static void printExceptions(ex_json, params_json, log, enums, limit=5) {
         def causingExceptions = ex_json['causingExceptions']
         if (causingExceptions.length() == 0) {
             def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/
@@ -345,11 +349,20 @@ class NfcoreSchema {
             else {
                 def param = ex_json['pointerToViolation'] - ~/^#\//
                 def param_val = params_json[param].toString()
-                log.error "* --${param}: ${ex_json['message']} (${param_val})"
+                if (enums.containsKey(param)) {
+                    def error_msg = "* --${param}: '${param_val}' is not a valid choice (Available choices"
+                    if (enums[param].size() > limit) {
+                        log.error "${error_msg} (${limit} of ${enums[param].size()}): ${enums[param][0..limit-1].join(', ')}, ... )"
+                    } else {
+                        log.error "${error_msg}: ${enums[param].join(', ')})"
+                    }
+                } else {
+                    log.error "* --${param}: ${ex_json['message']} (${param_val})"
+                }
             }
         }
         for (ex in causingExceptions) {
-            printExceptions(ex, params_json, log)
+            printExceptions(ex, params_json, log, enums)
         }
     }
 
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index 06499409..2fc0a9b9 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -24,7 +24,7 @@ class NfcoreTemplate {
     public static void checkConfigProvided(workflow, log) {
         if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
             log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
-                    "This will be dependent on your local compute enviroment but can be acheived via one or more of the following:\n" +
+                    "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
                     "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
                     "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
                     "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +

From 4d7f2168fbc9719561040c0eb6a02f676215f8e5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 11 Jan 2022 14:56:23 +0100
Subject: [PATCH 079/359] add logo

---
 assets/nf-core-rnafusion_logo_light.png | Bin 0 -> 10989 bytes
 1 file changed, 0 insertions(+), 0 deletions(-)
 create mode 100644 assets/nf-core-rnafusion_logo_light.png

diff --git a/assets/nf-core-rnafusion_logo_light.png b/assets/nf-core-rnafusion_logo_light.png
new file mode 100644
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kYW`ncg8$aa=zoH+7_+>)$kqx2?q3C|C}_%8y|N7de?P0f6951J

literal 0
HcmV?d00001


From 9931cd0bfebff6272a70e2cff77d7be8c7033ecc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 11 Jan 2022 16:39:40 +0100
Subject: [PATCH 080/359] Changed minimum nextflow version, input defaults for
 each pipeline part set to false

---
 .github/ISSUE_TEMPLATE/bug_report.yml      |  4 ++--
 .github/ISSUE_TEMPLATE/feature_request.yml |  2 +-
 .github/workflows/ci.yml                   | 23 ++++++++++++++--------
 README.md                                  |  4 ++--
 nextflow.config                            |  3 +--
 nextflow_schema.json                       | 23 +++++++++++-----------
 6 files changed, 32 insertions(+), 27 deletions(-)

diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index e904de57..a3de1c24 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -10,7 +10,7 @@ body:
         Before you post this issue, please check the documentation:
 
         - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
-        - [{{ name }} pipeline documentation](https://nf-co.re/{{ short_name }}/usage)
+        - [nf-core/rnafusion pipeline documentation](https://nf-co.re/rnafusion/usage)
 
   - type: textarea
     id: description
@@ -49,4 +49,4 @@ body:
         * Executor _(eg. slurm, local, awsbatch)_
         * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_
         * OS _(eg. CentOS Linux, macOS, Linux Mint)_
-        * Version of {{ name }} _(eg. 1.1, 1.5, 1.8.2)_
+        * Version of nf-core/rnafusion _(eg. 1.1, 1.5, 1.8.2)_
diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml
index ad3a715d..2c388d2f 100644
--- a/.github/ISSUE_TEMPLATE/feature_request.yml
+++ b/.github/ISSUE_TEMPLATE/feature_request.yml
@@ -1,5 +1,5 @@
 name: Feature request
-description: Suggest an idea for the {{ name }} pipeline
+description: Suggest an idea for the nf-core/rnafusion pipeline
 labels: enhancement
 body:
   - type: textarea
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index c826b074..4e2eb12a 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -8,8 +8,9 @@ on:
   release:
     types: [published]
 
-# Uncomment if we need an edge release of Nextflow again
-# env: NXF_EDGE: 1
+env:
+  NXF_ANSI_LOG: false
+  CAPSULE_LOG: none
 
 jobs:
   test:
@@ -17,20 +18,26 @@ jobs:
     # Only run on push if this is the nf-core dev branch (merged PRs)
     if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}
     runs-on: ubuntu-latest
-    env:
-      NXF_VER: ${{ matrix.nxf_ver }}
-      NXF_ANSI_LOG: false
     strategy:
       matrix:
-        # Nextflow versions: check pipeline minimum and current latest
-        nxf_ver: ['21.04.0', '']
+        # Nextflow versions
+        include:
+          # Test pipeline minimum Nextflow version
+          - NXF_VER: '21.10.3'
+            NXF_EDGE: ''
+          # Test latest edge release of Nextflow
+          - NXF_VER: ''
+            NXF_EDGE: '1'
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
 
       - name: Install Nextflow
         env:
-          CAPSULE_LOG: none
+          NXF_VER: ${{ matrix.NXF_VER }}
+          # Uncomment only if the edge release is more recent than the latest stable release
+          # See https://github.com/nextflow-io/nextflow/issues/2467
+          # NXF_EDGE: ${{ matrix.NXF_EDGE }}
         run: |
           wget -qO- get.nextflow.io | bash
           sudo mv nextflow /usr/local/bin/
diff --git a/README.md b/README.md
index 46a67684..1181a410 100644
--- a/README.md
+++ b/README.md
@@ -5,7 +5,7 @@
 [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)
 [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 
-[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.04.0-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
@@ -29,7 +29,7 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 ## Quick Start
 
-1. Install [`Nextflow`](https://nf-co.re/usage/installation) (`>=21.04.0`)
+1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`)
 
 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
 
diff --git a/nextflow.config b/nextflow.config
index 37dcecb6..0809068d 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -12,7 +12,6 @@ params {
     // Input options
     input                      = null
     fasta                      = null
-    // Build reference or analyse
     build_references           = false
 
     // MultiQC options
@@ -172,7 +171,7 @@ manifest {
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = 'RNA sequencing analysis pipeline with curated list of tools for detecting and visualizing fusion genes.'
     mainScript      = 'main.nf'
-    nextflowVersion = '!>=21.04.0'
+    nextflowVersion = '!>=21.10.3'
     version         = '1.0dev'
 }
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 34c39e4e..007999d5 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -57,17 +57,16 @@
                 "genomes_base": {
                     "type": "string",
                     "fa_icon": "fas fa-user-cog",
-                    "description": "Path to reference folder",
-                    "default": "GRCh38"
+                    "description": "Path to reference folder"
                 },
                 "ensembl_version": {
-                    "type": "string",
+                    "type": "integer",
                     "fa_icon": "fas fa-user-cog",
                     "description": "ensembl version",
                     "default": "97"
                 },
                 "read_length": {
-                    "type": "string",
+                    "type": "integer",
                     "fa_icon": "fas fa-user-cog",
                     "description": "Read length",
                     "default": "100"
@@ -76,49 +75,49 @@
                     "type": "boolean",
                     "fa_icon": "fas fa-user-cog",
                     "description": "Build or run all references/analyses",
-                    "default": "true"
+                    "default": "false"
                 },
                 "arriba": {
                     "type": "boolean",
                     "fa_icon": "fas fa-user-cog",
                     "description": "Build or run arriba references/analyses",
-                    "default": "true"
+                    "default": "false"
                 },
                 "ericscript": {
                     "type": "boolean",
                     "fa_icon": "fas fa-user-cog",
                     "description": "Build or run ericscript references/analyses",
-                    "default": "true"
+                    "default": "false"
                 },
                 "fusioncatcher": {
                     "type": "boolean",
                     "fa_icon": "fas fa-user-cog",
                     "description": "Build or run fusioncatcher references/analyses",
-                    "default": "true"
+                    "default": "false"
                 },
                 "pizzly": {
                     "type": "boolean",
                     "fa_icon": "fas fa-user-cog",
                     "description": "Build or run pizzly references/analyses",
-                    "default": "true"
+                    "default": "false"
                 },
                 "squid": {
                     "type": "boolean",
                     "fa_icon": "fas fa-user-cog",
                     "description": "Build or run squid references/analyses",
-                    "default": "true"
+                    "default": "false"
                 },
                 "starindex": {
                     "type": "boolean",
                     "fa_icon": "fas fa-user-cog",
                     "description": "Build or run starindex references/analyses",
-                    "default": "true"
+                    "default": "false"
                 },
                 "starfusion": {
                     "type": "boolean",
                     "fa_icon": "fas fa-user-cog",
                     "description": "Build or run starfusion references/analyses",
-                    "default": "true"
+                    "default": "false"
                 }
             }
         },

From f844ce13f53347501d8c37c2a66c46cf2e1747dd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 11 Jan 2022 17:30:58 +0100
Subject: [PATCH 081/359] Update awstests, remove schema's build_reference
 default adding igenome

---
 .github/workflows/awsfulltest.yml |   9 +-
 .github/workflows/awstest.yml     |  10 +-
 conf/igenomes.config              | 432 ++++++++++++++++++++++++++++++
 nextflow_schema.json              |  29 +-
 4 files changed, 455 insertions(+), 25 deletions(-)
 create mode 100644 conf/igenomes.config

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index ca680d0a..059d1680 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -14,11 +14,10 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@master
-
+        uses: nf-core/tower-action@v2
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
-          bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }}
+          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
           revision: ${{ github.sha }}
@@ -27,5 +26,5 @@ jobs:
             {
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
             }
-          profiles: '[ "test_full", "aws_tower" ]'
-
+          profiles: test_full,aws_tower
+          pre_run_script: 'export NXF_VER=21.10.3'
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index c3b9fbb4..2c933fcc 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -11,18 +11,18 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@master
+        uses: nf-core/tower-action@v2
         
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
-          bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }}
+          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
           revision: ${{ github.sha }}
           workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
           parameters: |
             {
-              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-test-${{ github.sha }}"
             }
-          profiles: '[ "test", "aws_tower" ]'
-
+          profiles: test,aws_tower
+          pre_run_script: 'export NXF_VER=21.10.3'
diff --git a/conf/igenomes.config b/conf/igenomes.config
new file mode 100644
index 00000000..6ff960c8
--- /dev/null
+++ b/conf/igenomes.config
@@ -0,0 +1,432 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for iGenomes paths
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines reference genomes using iGenome paths.
+    Can be used by any config that customises the base path using:
+        $params.igenomes_base / --igenomes_base
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    // illumina iGenomes reference file paths
+    genomes {
+        'GRCh37' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
+        }
+        'GRCh38' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
+        }
+        'GRCm38' {
+            fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "1.87e9"
+            blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
+        }
+        'TAIR10' {
+            fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
+            mito_name   = "Mt"
+        }
+        'EB2' {
+            fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
+        }
+        'UMD3.1' {
+            fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'WBcel235' {
+            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
+            mito_name   = "MtDNA"
+            macs_gsize  = "9e7"
+        }
+        'CanFam3.1' {
+            fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'GRCz10' {
+            fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'BDGP6' {
+            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
+            mito_name   = "M"
+            macs_gsize  = "1.2e8"
+        }
+        'EquCab2' {
+            fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'EB1' {
+            fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
+        }
+        'Galgal4' {
+            fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'Gm01' {
+            fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
+        }
+        'Mmul_1' {
+            fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'IRGSP-1.0' {
+            fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
+            mito_name   = "Mt"
+        }
+        'CHIMP2.1.4' {
+            fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'Rnor_5.0' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'Rnor_6.0' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+        }
+        'R64-1-1' {
+            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+            macs_gsize  = "1.2e7"
+        }
+        'EF2' {
+            fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "1.21e7"
+        }
+        'Sbi1' {
+            fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
+        }
+        'Sscrofa10.2' {
+            fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
+            mito_name   = "MT"
+        }
+        'AGPv3' {
+            fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
+            mito_name   = "Mt"
+        }
+        'hg38' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
+        }
+        'hg19' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
+        }
+        'mm10' {
+            fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "1.87e9"
+            blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
+        }
+        'bosTau8' {
+            fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+        }
+        'ce10' {
+            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "9e7"
+        }
+        'canFam3' {
+            fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'danRer10' {
+            fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "1.37e9"
+        }
+        'dm6' {
+            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "1.2e8"
+        }
+        'equCab2' {
+            fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'galGal4' {
+            fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'panTro4' {
+            fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+        'rn6' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+        }
+        'sacCer3' {
+            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
+            readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "1.2e7"
+        }
+        'susScr3' {
+            fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
+            bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
+            mito_name   = "chrM"
+        }
+    }
+}
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 007999d5..23557921 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -44,78 +44,77 @@
                 },
                 "build_references": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-user-cog",
-                    "description": "Specifies which analysis type for the pipeline - either build references or analyse data",
-                    "default": "false"
+                    "fa_icon": "far fa-file-code",
+                    "description": "Specifies which analysis type for the pipeline - either build references or analyse data"
                 },
                 "genome": {
                     "type": "string",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Which genome version",
                     "default": "GRCh38"
                 },
                 "genomes_base": {
                     "type": "string",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Path to reference folder"
                 },
                 "ensembl_version": {
                     "type": "integer",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "ensembl version",
                     "default": "97"
                 },
                 "read_length": {
                     "type": "integer",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Read length",
                     "default": "100"
                 },
                 "all": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Build or run all references/analyses",
                     "default": "false"
                 },
                 "arriba": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Build or run arriba references/analyses",
                     "default": "false"
                 },
                 "ericscript": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Build or run ericscript references/analyses",
                     "default": "false"
                 },
                 "fusioncatcher": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Build or run fusioncatcher references/analyses",
                     "default": "false"
                 },
                 "pizzly": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Build or run pizzly references/analyses",
                     "default": "false"
                 },
                 "squid": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Build or run squid references/analyses",
                     "default": "false"
                 },
                 "starindex": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Build or run starindex references/analyses",
                     "default": "false"
                 },
                 "starfusion": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-user-cog",
+                    "fa_icon": "far fa-file-code",
                     "description": "Build or run starfusion references/analyses",
                     "default": "false"
                 }

From 16d6fe2c8f7a1f135f25ca3c25d4752544c1ab4d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 11 Jan 2022 17:47:58 +0100
Subject: [PATCH 082/359] fix unchanged with nf-core 2.2

---
 lib/NfcoreSchema.groovy | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index b3d092f8..40ab65f2 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -27,7 +27,7 @@ class NfcoreSchema {
     /* groovylint-disable-next-line UnusedPrivateMethodParameter */
     public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') {
         def has_error = false
-        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
+        //=====================================================================//
         // Check for nextflow core params and unexpected params
         def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text
         def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
@@ -135,7 +135,7 @@ class NfcoreSchema {
             }
         }
 
-        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
+        //=====================================================================//
         // Validate parameters against the schema
         InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream()
         JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream))

From f27c4f89c68cad428d4fdb1dc76e2434a3c45d43 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 11 Jan 2022 17:57:37 +0100
Subject: [PATCH 083/359] input samplesheet in test and genome parameter in
 main

---
 conf/test.config      | 2 +-
 conf/test_full.config | 2 +-
 main.nf               | 2 ++
 3 files changed, 4 insertions(+), 2 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index d1efcef0..2f369996 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -20,7 +20,7 @@ params {
     // max_time   = 6.h
 
     // Input data
-    // input = 'assets/samplesheet_valid.csv'
+    input = 'assets/samplesheet_valid.csv'
 
     // Genome references
     // fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
diff --git a/conf/test_full.config b/conf/test_full.config
index 4304f216..fd17b10e 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -15,7 +15,7 @@ params {
     config_profile_description = 'Full test dataset to check pipeline function'
 
     // Input data
-    // input = 'assets/samplesheet_valid.csv'
+    input = 'assets/samplesheet_valid.csv'
 
     // Genome references
     // fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
diff --git a/main.nf b/main.nf
index 5fb70dfa..e0c1d702 100644
--- a/main.nf
+++ b/main.nf
@@ -18,6 +18,8 @@ nextflow.enable.dsl = 2
 */
 
 params.build_references           = WorkflowMain.getGenomeAttribute(params, 'build_references')
+params.genome                     = WorkflowMain.getGenomeAttribute(params, 'genome')
+
 /*
 ========================================================================================
     VALIDATE & PRINT PARAMETER SUMMARY

From 3e3ce28998c1594d78e2bbea2d662b28cf44dfcf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 12 Jan 2022 14:07:06 +0100
Subject: [PATCH 084/359] Add stub to ensembl and arriba download for build
 reference test

---
 modules/local/arriba/download/main.nf  | 10 +++++++-
 modules/local/ensembl/main.nf          | 10 ++++++++
 modules/local/get_software_versions.nf | 34 --------------------------
 3 files changed, 19 insertions(+), 35 deletions(-)
 delete mode 100644 modules/local/get_software_versions.nf

diff --git a/modules/local/arriba/download/main.nf b/modules/local/arriba/download/main.nf
index bfed085e..39afce76 100644
--- a/modules/local/arriba/download/main.nf
+++ b/modules/local/arriba/download/main.nf
@@ -13,7 +13,7 @@ process ARRIBA_DOWNLOAD {
     }
 
     output:
-    path "versions.yml"            , emit: versions
+    path "versions.yml"             , emit: versions
     path "arriba_v2.1.0/database/*" , emit: reference
 
     script:
@@ -24,4 +24,12 @@ process ARRIBA_DOWNLOAD {
 
     echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """
+
+    stub:
+    """
+    mkdir -p arriba_v2.1.0/database/
+    touch arriba_v2.1.0/database/arriba.test
+
+    touch versions.yml
+    """
 }
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index e9c7e5fa..516b07db 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -34,4 +34,14 @@ process ENSEMBL_DOWNLOAD {
 
     echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """
+
+    stub:
+    """
+    touch "Homo_sapiens.${params.genome}.${ensembl_version}.all.fa"
+    touch "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"
+    touch "Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz"
+
+    touch versions.yml
+    """
+
 }
diff --git a/modules/local/get_software_versions.nf b/modules/local/get_software_versions.nf
deleted file mode 100644
index d787cbfc..00000000
--- a/modules/local/get_software_versions.nf
+++ /dev/null
@@ -1,34 +0,0 @@
-// Import generic module functions
-include { saveFiles } from './functions'
-
-params.options = [:]
-
-process GET_SOFTWARE_VERSIONS {
-    label 'process_low'
-    publishDir "${params.outdir}",
-        mode: params.publish_dir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) }
-
-    conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/python:3.8.3"
-    } else {
-        container "quay.io/biocontainers/python:3.8.3"
-    }
-
-    cache false
-
-    input:
-    path versions
-
-    output:
-    path "software_versions.tsv"     , emit: tsv
-    path 'software_versions_mqc.yaml', emit: yaml
-
-    script: // This script is bundled with the pipeline, in nf-core/rnafusion/bin/
-    """
-    echo $workflow.manifest.version > pipeline.version.txt
-    echo $workflow.nextflow.version > nextflow.version.txt
-    scrape_software_versions.py &> software_versions_mqc.yaml
-    """
-}

From 027477f36cd989144bfb190e7be09a19d8f15f58 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 12 Jan 2022 14:19:05 +0100
Subject: [PATCH 085/359] use stub in test

---
 .github/workflows/ci.yml | 2 +-
 workflows/rnafusion.nf   | 1 -
 2 files changed, 1 insertion(+), 2 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 4e2eb12a..5b153a9b 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -44,4 +44,4 @@ jobs:
 
       - name: Run pipeline with test data
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,docker
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker -stub
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index db8075d2..4125e073 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -43,7 +43,6 @@ def fusioncatcher_genome_options = modules['fusioncatcher_download']
 
 // MODULE: Local to the pipeline
 //
-include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] )
 //
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //

From 37a2c79f980e514c620d1d74ad7e5fa5ea70f441 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 12 Jan 2022 14:19:40 +0100
Subject: [PATCH 086/359] add input parameters to tests

---
 conf/test.config      | 6 +++++-
 conf/test_full.config | 5 ++++-
 2 files changed, 9 insertions(+), 2 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 2f369996..2d5542fe 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -20,7 +20,11 @@ params {
     // max_time   = 6.h
 
     // Input data
-    input = 'assets/samplesheet_valid.csv'
+    input            = 'assets/samplesheet_valid.csv'
+    build_references = true
+    arriba           = true
+    genomes_base     = './results/references'
+
 
     // Genome references
     // fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
diff --git a/conf/test_full.config b/conf/test_full.config
index fd17b10e..f6604152 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -15,7 +15,10 @@ params {
     config_profile_description = 'Full test dataset to check pipeline function'
 
     // Input data
-    input = 'assets/samplesheet_valid.csv'
+    input                  = 'assets/samplesheet_valid.csv'
+    build_references       = true
+    arriba                 = true
+    genomes_base           = './results/references'
 
     // Genome references
     // fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'

From 8bcf2b230410fa3b76459f7c6acc418de1a5efdc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 12 Jan 2022 14:20:04 +0100
Subject: [PATCH 087/359] Update WorkflowMain.groovy

---
 lib/WorkflowMain.groovy | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index e15f4532..3266d8d4 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -60,6 +60,9 @@ class WorkflowMain {
         // Print parameter summary log to screen
         log.info paramsSummaryLog(workflow, params, log)
 
+        // Check that a -profile or Nextflow config has been provided to run the pipeline
+        NfcoreTemplate.checkConfigProvided(workflow, log)
+
         // Check that conda channels are set-up correctly
         if (params.enable_conda) {
             Utils.checkCondaChannels(log)
@@ -68,9 +71,6 @@ class WorkflowMain {
         // Check AWS batch settings
         NfcoreTemplate.awsBatch(workflow, params)
 
-        // Check the hostnames against configured profiles
-        NfcoreTemplate.hostName(workflow, params, log)
-
         // Check input has been provided
         if (!params.input) {
             log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'"

From 565243178be88e462184d5888d970c562419796e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 12 Jan 2022 14:20:51 +0100
Subject: [PATCH 088/359] Remove completely RNAFUSION process for testing
 purpose, before fixing it

---
 main.nf | 8 +-------
 1 file changed, 1 insertion(+), 7 deletions(-)

diff --git a/main.nf b/main.nf
index e0c1d702..26d6c09d 100644
--- a/main.nf
+++ b/main.nf
@@ -18,7 +18,6 @@ nextflow.enable.dsl = 2
 */
 
 params.build_references           = WorkflowMain.getGenomeAttribute(params, 'build_references')
-params.genome                     = WorkflowMain.getGenomeAttribute(params, 'genome')
 
 /*
 ========================================================================================
@@ -35,17 +34,12 @@ WorkflowMain.initialise(workflow, params, log)
 */
 
 include { BUILD_REFERENCES } from './workflows/build_references'
-include { RNAFUSION } from './workflows/rnafusion'
 
 //
 // WORKFLOW: Run main nf-core/rnafusion analysis pipeline
 //
 workflow NFCORE_RNAFUSION {
-    if (params.build_references) {
-        BUILD_REFERENCES ()
-    } else {
-        // RNAFUSION ()
-    }
+    BUILD_REFERENCES ()
 
 }
 

From 1388a0d6888083b64674af6c3038b54eb5781c1f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 12 Jan 2022 14:22:01 +0100
Subject: [PATCH 089/359] add param genomes default value and move genome to
 reference_genome_options

---
 nextflow.config      |  1 +
 nextflow_schema.json | 12 ++++++------
 2 files changed, 7 insertions(+), 6 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 0809068d..8f60174c 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -21,6 +21,7 @@ params {
 
     // Genome
     genome                     = 'GRCh38'
+    genomes                    = null
     genomes_base               = null
     ensembl_version            = 97
     read_length                = 100
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 23557921..f9bc9356 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -47,12 +47,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Specifies which analysis type for the pipeline - either build references or analyse data"
                 },
-                "genome": {
-                    "type": "string",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Which genome version",
-                    "default": "GRCh38"
-                },
                 "genomes_base": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -134,6 +128,12 @@
                     "description": "Path to FASTA genome file.",
                     "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.",
                     "fa_icon": "far fa-file-code"
+                },
+                "genome": {
+                    "type": "string",
+                    "description": "Name of iGenomes reference.",
+                    "fa_icon": "fas fa-book",
+                    "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details."
                 }
             }
         },

From fc1d0965b181bc49c650fb296a7109a98637c048 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 12 Jan 2022 14:27:55 +0100
Subject: [PATCH 090/359] limit cpu/memory/time to run on GitHub action

---
 conf/test.config      | 6 +++---
 conf/test_full.config | 5 +++++
 2 files changed, 8 insertions(+), 3 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 2d5542fe..8424b6e4 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -15,9 +15,9 @@ params {
     config_profile_description = 'Minimal test dataset to check pipeline function'
 
     // Limit resources so that this can run on GitHub Actions
-    // max_cpus   = 2
-    // max_memory = 6.GB
-    // max_time   = 6.h
+    max_cpus   = 2
+    max_memory = 6.GB
+    max_time   = 6.h
 
     // Input data
     input            = 'assets/samplesheet_valid.csv'
diff --git a/conf/test_full.config b/conf/test_full.config
index f6604152..73b539a4 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -14,6 +14,11 @@ params {
     config_profile_name        = 'Full test profile'
     config_profile_description = 'Full test dataset to check pipeline function'
 
+    // Limit resources so that this can run on GitHub Actions
+    max_cpus   = 2
+    max_memory = 6.GB
+    max_time   = 6.h
+
     // Input data
     input                  = 'assets/samplesheet_valid.csv'
     build_references       = true

From 909e17d7025e10482b9e0b7e69fa7e6cb78d3018 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 17 Jan 2022 11:16:47 +0100
Subject: [PATCH 091/359] linting

---
 CHANGELOG.md  |  24 ++++-----
 README.md     |   2 -
 docs/usage.md | 140 +++++++++++++++++++++++++-------------------------
 3 files changed, 82 insertions(+), 84 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 1dfdae43..a8e91fbe 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -37,20 +37,20 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ## [1.1.0] nfcore/rnafusion - 2020/02/10
 
 * Fusion gene detection tools:
-  * `Arriba v1.1.0`
-  * `Ericscript v0.5.5`
-  * `Fusioncatcher v1.20`
-  * `Pizzly v0.37.3`
-  * `Squid v1.5`
-  * `STAR-Fusion v1.6.0`
+    * `Arriba v1.1.0`
+    * `Ericscript v0.5.5`
+    * `Fusioncatcher v1.20`
+    * `Pizzly v0.37.3`
+    * `Squid v1.5`
+    * `STAR-Fusion v1.6.0`
 * Visualization tools:
-  * `Arriba v1.1.0`
-  * `FusionInspector v1.3.1`
+    * `Arriba v1.1.0`
+    * `FusionInspector v1.3.1`
 * Other tools:
-  * `fusion-report v2.0.1`
-  * `FastQ v0.11.8`
-  * `MultiQC v1.7`
-  * `STAR aligner v2.7.0f`
+    * `fusion-report v2.0.1`
+    * `FastQ v0.11.8`
+    * `MultiQC v1.7`
+    * `STAR aligner v2.7.0f`
 
 ### Added
 
diff --git a/README.md b/README.md
index 0a10e6ea..266a1e0b 100644
--- a/README.md
+++ b/README.md
@@ -31,8 +31,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 | [Squid](https://github.com/Kingsford-Group/squid)                         |         :x:        |   `1.5`  |
 | [Star-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: |  `1.9.1` |
 
-## Quick Start
-
 ## Pipeline summary
 
 <!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
diff --git a/docs/usage.md b/docs/usage.md
index 3d7d8ded..d49a6cb4 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -9,59 +9,59 @@
 - [:warning: Please read this documentation on the nf-core website: https://nf-co.re/rnafusion/usage](#warning-please-read-this-documentation-on-the-nf-core-website-httpsnf-corernafusionusage)
 - [Introduction](#introduction)
 - [Download references](#download-references)
-  - [Download all references](#download-all-references)
-  - [Download specific references](#download-specific-references)
-  - [Download and build CTAT](#download-and-build-ctat)
-  - [Download GRCh37 references](#download-grch37-references)
-  - [Tool reference requirements](#tool-reference-requirements)
+    - [Download all references](#download-all-references)
+    - [Download specific references](#download-specific-references)
+    - [Download and build CTAT](#download-and-build-ctat)
+    - [Download GRCh37 references](#download-grch37-references)
+    - [Tool reference requirements](#tool-reference-requirements)
 - [Running the pipeline](#running-the-pipeline)
-  - [Updating the pipeline](#updating-the-pipeline)
-  - [Reproducibility](#reproducibility)
+    - [Updating the pipeline](#updating-the-pipeline)
+    - [Reproducibility](#reproducibility)
 - [Core Nextflow arguments](#core-nextflow-arguments)
-  - [`-profile`](#-profile)
-  - [`-resume`](#-resume)
-  - [`-c`](#-c)
-    - [Custom resource requests](#custom-resource-requests)
-  - [Running in the background](#running-in-the-background)
-    - [Nextflow memory requirements](#nextflow-memory-requirements)
+    - [`-profile`](#-profile)
+    - [`-resume`](#-resume)
+    - [`-c`](#-c)
+        - [Custom resource requests](#custom-resource-requests)
+    - [Running in the background](#running-in-the-background)
+        - [Nextflow memory requirements](#nextflow-memory-requirements)
 - [Pipeline specific arguments](#pipeline-specific-arguments)
-  - [--input](#--input)
-  - [--single_end](#--single_end)
-  - [Tool flags](#tool-flags)
-  - [--arriba](#--arriba)
-  - [--ericscript](#--ericscript)
-  - [--fusioncatcher](#--fusioncatcher)
-  - [--fusion_report](#--fusion_report)
-  - [--pizzly](#--pizzly)
-  - [--squid](#--squid)
-  - [--star_fusion](#--star_fusion)
+    - [--input](#--input)
+    - [--single_end](#--single_end)
+    - [Tool flags](#tool-flags)
+    - [--arriba](#--arriba)
+    - [--ericscript](#--ericscript)
+    - [--fusioncatcher](#--fusioncatcher)
+    - [--fusion_report](#--fusion_report)
+    - [--pizzly](#--pizzly)
+    - [--squid](#--squid)
+    - [--star_fusion](#--star_fusion)
 - [Visualization flags](#visualization-flags)
-  - [--arriba_vis](#--arriba_vis)
-  - [--fusion_inspector](#--fusion_inspector)
+    - [--arriba_vis](#--arriba_vis)
+    - [--fusion_inspector](#--fusion_inspector)
 - [Reference genomes](#reference-genomes)
-  - [--arriba_ref](#--arriba_ref)
-  - [--databases](#--databases)
-  - [--ericscript_ref](#--ericscript_ref)
-  - [--fasta](#--fasta)
-  - [--fusioncatcher_ref](#--fusioncatcher_ref)
-  - [--genome](#--genome)
-  - [--gtf](#--gtf)
-  - [--reference_release](#--reference_release)
-  - [--star_index](#--star_index)
-  - [--star_fusion_ref](#--star_fusion_ref)
-  - [--transcript](#--transcript)
+    - [--arriba_ref](#--arriba_ref)
+    - [--databases](#--databases)
+    - [--ericscript_ref](#--ericscript_ref)
+    - [--fasta](#--fasta)
+    - [--fusioncatcher_ref](#--fusioncatcher_ref)
+    - [--genome](#--genome)
+    - [--gtf](#--gtf)
+    - [--reference_release](#--reference_release)
+    - [--star_index](#--star_index)
+    - [--star_fusion_ref](#--star_fusion_ref)
+    - [--transcript](#--transcript)
 - [Other command line parameters](#other-command-line-parameters)
-  - [--debug](#--debug)
-  - [--read_length](#--read_length)
-  - [--outdir](#--outdir)
-  - [--email](#--email)
-  - [--email_on_fail](#--email_on_fail)
-  - [--max_multiqc_email_size](#--max_multiqc_email_size)
-  - [-name](#-name)
-  - [--custom_config_version](#--custom_config_version)
-  - [--custom_config_base](#--custom_config_base)
+    - [--debug](#--debug)
+    - [--read_length](#--read_length)
+    - [--outdir](#--outdir)
+    - [--email](#--email)
+    - [--email_on_fail](#--email_on_fail)
+    - [--max_multiqc_email_size](#--max_multiqc_email_size)
+    - [-name](#-name)
+    - [--custom_config_version](#--custom_config_version)
+    - [--custom_config_base](#--custom_config_base)
 - [Job resources](#job-resources)
-  - [Automatic resubmission](#automatic-resubmission)
+    - [Automatic resubmission](#automatic-resubmission)
 
 ## Download references
 
@@ -257,21 +257,21 @@ They are loaded in sequence, so later profiles can overwrite earlier profiles.
 
 If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended.
 
-* `docker`
-    * A generic configuration profile to be used with [Docker](https://docker.com/)
-* `singularity`
-    * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
-* `podman`
-    * A generic configuration profile to be used with [Podman](https://podman.io/)
-* `shifter`
-    * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
-* `charliecloud`
-    * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
-* `conda`
-    * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
-* `test`
-    * A profile with a complete configuration for automated testing
-    * Includes links to test data so needs no other parameters
+- `docker`
+    - A generic configuration profile to be used with [Docker](https://docker.com/)
+- `singularity`
+    - A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
+- `podman`
+    - A generic configuration profile to be used with [Podman](https://podman.io/)
+- `shifter`
+    - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
+- `charliecloud`
+    - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
+- `conda`
+    - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
+- `test`
+    - A profile with a complete configuration for automated testing
+    - Includes links to test data so needs no other parameters
 
 ### `-resume`
 
@@ -340,7 +340,7 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
 2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
 3. Create the custom config accordingly:
 
-    * For Docker:
+    - For Docker:
 
         ```nextflow
         process {
@@ -350,7 +350,7 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
         }
         ```
 
-    * For Singularity:
+    - For Singularity:
 
         ```nextflow
         process {
@@ -360,7 +360,7 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
         }
         ```
 
-    * For Conda:
+    - For Conda:
 
         ```nextflow
         process {
@@ -431,14 +431,14 @@ By default, the pipeline expects paired-end data. If you have single-end data, y
 If enabled, executes `Arriba` tool.
 
 - `--arriba_opt`
-  - Specify additional parameters. For more information, please refer to the [documentation](http://arriba.readthedocs.io/en/latest/quickstart/) of the tool.
+    - Specify additional parameters. For more information, please refer to the [documentation](http://arriba.readthedocs.io/en/latest/quickstart/) of the tool.
 
 ### --ericscript
 
 If enabled, executes `Ericscript` tool.
 
 - `--ericscript_opt`
-  - Specify additional parameters. For more information, please refer to the [documentation](https://sites.google.com/site/bioericscript/home) of the tool.
+    - Specify additional parameters. For more information, please refer to the [documentation](https://sites.google.com/site/bioericscript/home) of the tool.
 
 ### --fusioncatcher
 
@@ -447,21 +447,21 @@ If enabled, executes `Fusioncatcher` tool.
 > N.B. that Fusioncatcher is not available when using the `GRCh37` genome assembly.
 
 - `--fusioncatcher_opt`
-  - Specify additional parameters. For more information, please refer to the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md) of the tool.
+    - Specify additional parameters. For more information, please refer to the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md) of the tool.
 
 ### --fusion_report
 
 If enabled, download databases for `fusion-report`.
 
 - `fusion_report_opt`
-  - Specify additional parameters. For more information, please refer to the [documentation](https://matq007.github.io/fusion-report/#/) of the tool.
+    - Specify additional parameters. For more information, please refer to the [documentation](https://matq007.github.io/fusion-report/#/) of the tool.
 
 ### --pizzly
 
 If enabled, executes `Pizzly` tool.
 
 - `--pizzly_k`
-  - Number of k-mers. Default `31`.
+    - Number of k-mers. Default `31`.
 
 ### --squid
 
@@ -472,7 +472,7 @@ If enabled, executes `Squid` tool.
 If enabled, executes `STAR-Fusion` tool.
 
 - `--star_fusion_opt`
-  - Parameter for specifying additional parameters. For more information, please refer to the [documentation](https://github.com/STAR-Fusion/STAR-Fusion/wiki) of the tool.
+    - Parameter for specifying additional parameters. For more information, please refer to the [documentation](https://github.com/STAR-Fusion/STAR-Fusion/wiki) of the tool.
 
 ## Visualization flags
 

From b742e186f796e3c98bc7fb346be2689340b929b5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 17 Jan 2022 11:26:02 +0100
Subject: [PATCH 092/359] intendation and final newline fixes

---
 build-ctat.nf                         | 26 +++++++++++-----------
 conf/base.config                      | 16 +++++++-------
 containers/arriba/Dockerfile          |  2 +-
 containers/arriba/environment.yml     |  2 +-
 containers/ericscript/Dockerfile      |  2 +-
 containers/ericscript/environment.yml |  2 +-
 containers/fusioncatcher/Dockerfile   |  2 +-
 containers/pizzly/Dockerfile          |  4 ++--
 containers/squid/Dockerfile           |  2 +-
 docs/usage.md                         | 32 +++++++++++++--------------
 scripts/build.sh                      |  2 +-
 11 files changed, 46 insertions(+), 46 deletions(-)

diff --git a/build-ctat.nf b/build-ctat.nf
index 272e9e83..8df8d500 100644
--- a/build-ctat.nf
+++ b/build-ctat.nf
@@ -4,16 +4,16 @@
                                 nf-core/rnafusion
 ================================================================================
 nf-core/rnafusion:
- RNA-seq analysis pipeline for detection gene-fusions
+RNA-seq analysis pipeline for detection gene-fusions
 --------------------------------------------------------------------------------
- @Homepage
- https://nf-co.re/rnafusion
+@Homepage
+https://nf-co.re/rnafusion
 --------------------------------------------------------------------------------
- @Documentation
- https://nf-co.re/rnafusion/docs
+@Documentation
+https://nf-co.re/rnafusion/docs
 --------------------------------------------------------------------------------
- @Repository
- https://github.com/nf-core/rnafusion
+@Repository
+https://github.com/nf-core/rnafusion
 --------------------------------------------------------------------------------
 */
 
@@ -27,11 +27,11 @@ def helpMessage() {
     nextflow run nf-core/rnafusion/build-ctat.nf -profile [PROFILE] [OPTIONS] --outdir /path/to/output
 
     Mandatory arguments:
-      --fasta [file]                Path to fasta reference
-      --gtf [file]                  Path to GTF annotation
-      --genome [str]                Either "GRCh37" or "GRCh38" (default)
-      --outdir [path]               Output directory for downloading
-      -profile [str]                Configuration profile [https://github.com/nf-core/configs]
+        --fasta [file]                Path to fasta reference
+        --gtf [file]                  Path to GTF annotation
+        --genome [str]                Either "GRCh37" or "GRCh38" (default)
+        --outdir [path]               Output directory for downloading
+        -profile [str]                Configuration profile [https://github.com/nf-core/configs]
     """.stripIndent()
 }
 
@@ -67,7 +67,7 @@ checkHostname()
 
 /*
 ================================================================================
-                                  DOWNLOAD
+DOWNLOAD
 ================================================================================
 */
 
diff --git a/conf/base.config b/conf/base.config
index 31d95742..63cef552 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -10,14 +10,14 @@
 
 params {
     versions {
-      arriba = '1.2.0'
-      ericscript = '0.5.5'
-      fusioncatcher = '1.20'
-      pizzly = '0.37.3'
-      squid = '1.5-star2.7.1a'
-      star_fusion = '1.9.1'
-      fusion_inspector = '2.3.1'
-  }
+        arriba = '1.2.0'
+        ericscript = '0.5.5'
+        fusioncatcher = '1.20'
+        pizzly = '0.37.3'
+        squid = '1.5-star2.7.1a'
+        star_fusion = '1.9.1'
+        fusion_inspector = '2.3.1'
+    }
 }
 
 process {
diff --git a/containers/arriba/Dockerfile b/containers/arriba/Dockerfile
index f6a94950..ea36d90f 100644
--- a/containers/arriba/Dockerfile
+++ b/containers/arriba/Dockerfile
@@ -11,4 +11,4 @@ RUN conda env create -f /environment.yml && conda clean -a
 ENV PATH /opt/conda/envs/nf-core-rnafusion-arriba_1.2.0/bin:$PATH
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-arriba_1.2.0 > nf-core-rnafusion-arriba_1.2.0.yml
\ No newline at end of file
+RUN conda env export --name nf-core-rnafusion-arriba_1.2.0 > nf-core-rnafusion-arriba_1.2.0.yml
diff --git a/containers/arriba/environment.yml b/containers/arriba/environment.yml
index 393b2e11..20a1024e 100644
--- a/containers/arriba/environment.yml
+++ b/containers/arriba/environment.yml
@@ -11,4 +11,4 @@ dependencies:
   - bioconda::star=2.7.1a
   - conda-forge::openssl=1.0
   - conda-forge::r-circlize
-  - conda-forge::readline=6.2
\ No newline at end of file
+  - conda-forge::readline=6.2
diff --git a/containers/ericscript/Dockerfile b/containers/ericscript/Dockerfile
index d418d1c7..70b87c48 100644
--- a/containers/ericscript/Dockerfile
+++ b/containers/ericscript/Dockerfile
@@ -14,4 +14,4 @@ ENV PATH /opt/conda/envs/nf-core-rnafusion-ericscript_0.5.5/bin:$PATH
 RUN echo 1 > /opt/conda/envs/nf-core-rnafusion-ericscript_0.5.5/share/ericscript-0.5.5-4/lib/data/_resources/.flag.dbexists
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-ericscript_0.5.5 > nf-core-rnafusion-ericscript_0.5.5.yml
\ No newline at end of file
+RUN conda env export --name nf-core-rnafusion-ericscript_0.5.5 > nf-core-rnafusion-ericscript_0.5.5.yml
diff --git a/containers/ericscript/environment.yml b/containers/ericscript/environment.yml
index 3edc48ce..1e76273c 100644
--- a/containers/ericscript/environment.yml
+++ b/containers/ericscript/environment.yml
@@ -5,4 +5,4 @@ channels:
   - defaults
 dependencies:
   - bioconda::ericscript=0.5.5
-  - conda-forge::ncurses=6.1
\ No newline at end of file
+  - conda-forge::ncurses=6.1
diff --git a/containers/fusioncatcher/Dockerfile b/containers/fusioncatcher/Dockerfile
index 203bb32a..b713f35c 100644
--- a/containers/fusioncatcher/Dockerfile
+++ b/containers/fusioncatcher/Dockerfile
@@ -11,4 +11,4 @@ RUN conda env create -f /environment.yml && conda clean -a
 ENV PATH /opt/conda/envs/nf-core-rnafusion-fusioncatcher_1.20/bin:$PATH
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-fusioncatcher_1.20 > nf-core-rnafusion-fusioncatcher_1.20.yml
\ No newline at end of file
+RUN conda env export --name nf-core-rnafusion-fusioncatcher_1.20 > nf-core-rnafusion-fusioncatcher_1.20.yml
diff --git a/containers/pizzly/Dockerfile b/containers/pizzly/Dockerfile
index 2817069f..bc16bc85 100644
--- a/containers/pizzly/Dockerfile
+++ b/containers/pizzly/Dockerfile
@@ -1,4 +1,4 @@
-FROM nfcore/base:1.9
+containers/pizzly/Dockerfile FROM nfcore/base:1.9
 
 LABEL authors="Martin Proks" \
     description="Docker image containing all requirements for nfcore/rnafusion pipeline"
@@ -11,4 +11,4 @@ RUN conda env create -f /environment.yml && conda clean -a
 ENV PATH /opt/conda/envs/nf-core-rnafusion-pizzly_0.37.3/bin:$PATH
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-pizzly_0.37.3 > nf-core-rnafusion-pizzly_0.37.3.yml
\ No newline at end of file
+RUN conda env export --name nf-core-rnafusion-pizzly_0.37.3 > nf-core-rnafusion-pizzly_0.37.3.yml
diff --git a/containers/squid/Dockerfile b/containers/squid/Dockerfile
index 5dbcaf4c..20d390ee 100644
--- a/containers/squid/Dockerfile
+++ b/containers/squid/Dockerfile
@@ -16,4 +16,4 @@ RUN cd /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin \
     && ln -s /opt/conda/envs/nf-core-rnafusion-squid_1.5-star2.7.1a/bin/python3 /bin/python
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-squid_1.5-star2.7.1a > nf-core-rnafusion-squid_1.5-star2.7.1a.yml
\ No newline at end of file
+RUN conda env export --name nf-core-rnafusion-squid_1.5-star2.7.1a > nf-core-rnafusion-squid_1.5-star2.7.1a.yml
diff --git a/docs/usage.md b/docs/usage.md
index d49a6cb4..07db3570 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -77,9 +77,9 @@ To make the pipeline easier to work with, we provide a script to download all ne
 ```bash
 # Replace <COSMIC_USER> and <COSMIC_PASSWD> with yout credentials from COSMIC
 nextflow run nf-core/rnafusion/download-references.nf \
-  --download_all \
-  --outdir <PATH> \
-  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+--download_all \
+--outdir <PATH> \
+--cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
 ```
 
 ### Download specific references
@@ -112,17 +112,17 @@ nextflow run nf-core/rnafusion/download-references.nf \
 nextflow run nf-core/rnafusion/download-references.nf \
 --fusion_report \
 --outdir <PATH>
-  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+--cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
 ```
 
 ### Download and build CTAT
 
 ```bash
 nextflow run nf-core/rnafusion/build-ctat.nf \
-  --genome GRCh38 \
-  --outdir <PATH> \
-  --fasta <PATH>/<FASTA \
-  --gtf <PATH>/<GTF>
+--genome GRCh38 \
+--outdir <PATH> \
+--fasta <PATH>/<FASTA \
+--gtf <PATH>/<GTF>
 ```
 
 ### Download GRCh37 references
@@ -131,19 +131,19 @@ nextflow run nf-core/rnafusion/build-ctat.nf \
 # GRCh38 genome assembly is used by default.
 # To use the previous assembly specify it using the --genome flag
 nextflow run nf-core/rnafusion/download-references.nf \
-  --genome GRCh37 \
-  --download_all \
-  --outdir <PATH> \
-  --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+--genome GRCh37 \
+--download_all \
+--outdir <PATH> \
+--cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
 
 # Please note that using the above example command downloads NCBI-based references for STAR-Fusion.
 # To use Ensembl-based references run the following command with the same <PATH> as used above
 
 nextflow run nf-core/rnafusion/build-ctat.nf \
-  --genome GRCh37 \
-  --outdir <PATH> \
-  --fasta <PATH>/<FASTA> \
-  --gtf <PATH>/<GTF>
+--genome GRCh37 \
+--outdir <PATH> \
+--fasta <PATH>/<FASTA> \
+--gtf <PATH>/<GTF>
 ```
 
 ### Tool reference requirements
diff --git a/scripts/build.sh b/scripts/build.sh
index 5c439c43..626542e5 100755
--- a/scripts/build.sh
+++ b/scripts/build.sh
@@ -21,7 +21,7 @@ fi
 if [ $1 == "-h" ]; then
     echo "Utility for building docker containers from tools/"
     echo "Usage: scripts/build.sh [options]"
-    echo 
+    echo
     echo "Options:"
     echo "  all             build all tools including main image"
     echo "  <tool name>     builds specific tool"

From 80154e1c97a72daf235e6d53f51f624bad91b0d2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 17 Jan 2022 11:36:57 +0100
Subject: [PATCH 093/359] remove download-references.nf

---
 download-references.nf | 260 -----------------------------------------
 1 file changed, 260 deletions(-)
 delete mode 100644 download-references.nf

diff --git a/download-references.nf b/download-references.nf
deleted file mode 100644
index 4dd34b66..00000000
--- a/download-references.nf
+++ /dev/null
@@ -1,260 +0,0 @@
-#!/usr/bin/env nextflow
-/*
-================================================================================
-                                nf-core/rnafusion
-================================================================================
-nf-core/rnafusion:
- RNA-seq analysis pipeline for detection gene-fusions
---------------------------------------------------------------------------------
- @Homepage
- https://nf-co.re/rnafusion
---------------------------------------------------------------------------------
- @Documentation
- https://nf-co.re/rnafusion/docs
---------------------------------------------------------------------------------
- @Repository
- https://github.com/nf-core/rnafusion
---------------------------------------------------------------------------------
-*/
-
-def helpMessage() {
-    log.info nfcoreHeader()
-    log.info"""
-    Usage:
-
-    The typical command for downloading references is as follows:
-
-    nextflow run nf-core/rnafusion/download-references.nf -profile [PROFILE] [OPTIONS] --outdir /path/to/output
-
-    Mandatory arguments:
-      --outdir [path]               Output directory for downloading
-
-    Options:
-      --download_all [bool]         Download all references
-      --genome [str]                Genome version: GRCh37 or GRCh38 (default)
-      --reference_release [int]     Release number of Ensembl reference for FASTA and GTF
-                                    Default: 97 -> ftp://ftp.ensembl.org/pub/release-97
-      --base [bool]                 Download FASTA, GTF, cDNA
-      --arriba [bool]               Download Arriba references
-      --star_fusion [bool]          Build STAR-Fusion references from FASTA ANF GTF
-      --fusioncatcher [bool]        Download Fusioncatcher references
-      --ericscript [bool]           Download Ericscript references
-      --fusion_report [bool]        Download databases for fusion-report
-      --cosmic_usr [str]            [Required with fusion_report] COSMIC username
-      --cosmic_passwd [str]         [Required with fusion_report] COSMIC password
-    """.stripIndent()
-}
-
-/*
- * SET UP CONFIGURATION VARIABLES
- */
-
-// Show help emssage
-if (params.help) exit 0, helpMessage()
-if (!params.outdir) exit 1, "Output directory not specified!"
-
-running_tools = []
-if (params.base || params.download_all) running_tools.add("Reference v${params.reference_release}")
-if (params.arriba || params.download_all) running_tools.add("Arriba")
-if (params.star_fusion || params.download_all) running_tools.add("STAR-Fusion")
-if (params.fusioncatcher || params.download_all) running_tools.add("Fusioncatcher")
-if (params.ericscript || params.download_all) running_tools.add("Ericscript")
-if (params.fusion_report || params.download_all) {
-    running_tools.add('fusion-report')
-    if (!params.cosmic_usr || !params.cosmic_passwd) exit 1, "Database credentials are required parameter!"
-}
-
-// Header log info
-log.info nfcoreHeader()
-def summary = [:]
-summary['Pipeline Name']  = 'nf-core/rnafusion/download-references.nf'
-summary['Pipeline Version'] = workflow.manifest.version
-summary['References']       = running_tools.size() == 0 ? 'None' : running_tools.join(", ")
-if(workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container"
-summary['Max Resources']    = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job"
-summary['Output dir']   = params.outdir
-summary['User']         = workflow.userName
-log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n")
-log.info "\033[2m----------------------------------------------------\033[0m"
-
-// Check the hostnames against configured profiles
-checkHostname()
-
-/*
-================================================================================
-                                  DOWNLOAD
-================================================================================
-*/
-
-process download_base {
-    publishDir "${params.outdir}/", mode: 'copy'
-
-    when:
-    params.base || params.download_all
-
-    output:
-    file "Homo_sapiens.${params.genome}_r${params.reference_release}.all.fa" into fasta
-    file "Homo_sapiens.${params.genome}_r${params.reference_release}.gtf" into gtf
-    file "Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz" into transcript
-
-    script:
-    def base_url = params.genome == "GRCh37" ? "ftp://ftp.ensembl.org/pub/grch37" : "ftp://ftp.ensembl.org/pub/"
-    """
-    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
-    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
-    gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}_r${params.reference_release}.all.fa
-    wget ${base_url}/release-${params.reference_release}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${params.reference_release}.chr.gtf.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
-    gunzip Homo_sapiens.${params.genome}_r${params.reference_release}.gtf.gz
-    wget ${base_url}/release-${params.reference_release}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}_r${params.reference_release}.cdna.all.fa.gz
-    """
-}
-
-process download_arriba {
-    publishDir "${params.outdir}/arriba", mode: 'copy'
-
-    when:
-    params.arriba || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    """
-    wget -N https://github.com/suhrig/arriba/releases/download/v1.2.0/arriba_v1.2.0.tar.gz -O arriba_v1.2.0.tar.gz
-    tar -xvzf arriba_v1.2.0.tar.gz && mv arriba_v1.2.0/database/* . && gunzip *.gz && rm -rf arriba_*
-    """
-}
-
-process download_star_fusion {
-    publishDir "${params.outdir}/star-fusion", mode: 'copy'
-
-    when:
-    params.star_fusion || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    """
-    if [[ ${params.genome} == "GRCh37" ]]; then
-      wget -O GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.9/GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz
-      tar -xf GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz --strip-components=5
-      rm GRCh37_gencode_v19_CTAT_lib_Apr032020.plug-n-play.tar.gz
-    else
-      aws s3 --no-sign-request --region ${params.awsregion} cp s3://ngi-igenomes/Homo_sapiens/Ensembl/GRCh38/Genome/CTAT/ctat_star_fusion_1_8_1.tar.gz .
-      tar -xf ctat_star_fusion_1_8_1.tar.gz --strip-components=5
-      rm ctat_star_fusion_1_8_1.tar.gz
-    fi
-    """
-}
-
-process download_fusioncatcher {
-    publishDir "${params.outdir}/fusioncatcher", mode: 'copy'
-
-    when:
-    params.fusioncatcher || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    """
-    if [[ ${params.genome} == "GRCh37" ]]; then
-      echo "FusionCatcher unavailable for GRCh37" > README.txt
-    else
-      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.aa
-      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ab
-      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ac
-      wget -N http://sourceforge.net/projects/fusioncatcher/files/data/human_v98.tar.gz.ad
-      cat human_v98.tar.gz.* | tar xz
-      rm human_v98.tar*
-    fi
-    """
-}
-
-process download_ericscript {
-    publishDir "${params.outdir}/ericscript", mode: 'copy'
-
-    when:
-    params.ericscript || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    def base_url = params.genome == "GRCh37" ? "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIibDRIb0RFdHFlQmM" : "https://drive.google.com/uc?export=download&confirm=qgOc&id=0B9s__vuJPvIiUGt1SnFMZFg4TlE"
-    """
-    if [[ ${params.genome} == "GRCh37" ]]; then
-      gdown ${base_url} ericscript_db_homosapiens_ensembl73.tar.bz2
-    else
-      gdown ${base_url} ericscript_db_homosapiens_ensembl84.tar.bz2
-    fi
-    tar jxf ericscript_db_homosapiens_ensembl*.tar.bz2 && rm ericscript_db_homosapiens_ensembl*.tar.bz2
-    """
-}
-
-process download_databases {
-    publishDir "${params.outdir}/databases", mode: 'copy'
-
-    when:
-    params.fusion_report || params.download_all
-
-    output:
-    file '*'
-
-    script:
-    """
-    fusion_report download --cosmic_usr "${params.cosmic_usr}" --cosmic_passwd "${params.cosmic_passwd}" .
-    """
-}
-
-/*
- * Completion
- */
-workflow.onComplete {
-    log.info "[nf-core/rnafusion/download-references.nf] Pipeline Complete"
-}
-
-def nfcoreHeader() {
-    // Log colors ANSI codes
-    c_black = params.monochrome_logs ? '' : "\033[0;30m";
-    c_blue = params.monochrome_logs ? '' : "\033[0;34m";
-    c_cyan = params.monochrome_logs ? '' : "\033[0;36m";
-    c_dim = params.monochrome_logs ? '' : "\033[2m";
-    c_green = params.monochrome_logs ? '' : "\033[0;32m";
-    c_purple = params.monochrome_logs ? '' : "\033[0;35m";
-    c_reset = params.monochrome_logs ? '' : "\033[0m";
-    c_white = params.monochrome_logs ? '' : "\033[0;37m";
-    c_yellow = params.monochrome_logs ? '' : "\033[0;33m";
-
-    return """    -${c_dim}--------------------------------------------------${c_reset}-
-                                            ${c_green},--.${c_black}/${c_green},-.${c_reset}
-    ${c_blue}        ___     __   __   __   ___     ${c_green}/,-._.--~\'${c_reset}
-    ${c_blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${c_yellow}}  {${c_reset}
-    ${c_blue}  | \\| |       \\__, \\__/ |  \\ |___     ${c_green}\\`-._,-`-,${c_reset}
-                                            ${c_green}`._,._,\'${c_reset}
-    ${c_purple}  nf-core/rnafusion v${workflow.manifest.version}${c_reset}
-    -${c_dim}--------------------------------------------------${c_reset}-
-    """.stripIndent()
-}
-
-def checkHostname() {
-    def c_reset = params.monochrome_logs ? '' : "\033[0m"
-    def c_white = params.monochrome_logs ? '' : "\033[0;37m"
-    def c_red = params.monochrome_logs ? '' : "\033[1;91m"
-    def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m"
-    if (params.hostnames) {
-        def hostname = "hostname".execute().text.trim()
-        params.hostnames.each { prof, hnames ->
-            hnames.each { hname ->
-                if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                    log.error "====================================================\n" +
-                            "  ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" +
-                            "  but your machine hostname is ${c_white}'$hostname'${c_reset}\n" +
-                            "  ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" +
-                            "============================================================"
-                }
-            }
-        }
-    }
-}

From 771200490c245f6f0725d065db1279737a696dd3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 17 Jan 2022 16:32:34 +0100
Subject: [PATCH 094/359] nf-core lint --fix files_unchanged and ensembl
 version

---
 assets/nf-core-rnafusion_logo_light.png      | Bin 288 -> 10989 bytes
 conf/genomes.config                          |  12 ++++++------
 docs/images/nf-core-rnafusion_logo_dark.png  | Bin 288 -> 72761 bytes
 docs/images/nf-core-rnafusion_logo_light.png | Bin 288 -> 72629 bytes
 docs/usage.md                                |  11 -----------
 5 files changed, 6 insertions(+), 17 deletions(-)

diff --git a/assets/nf-core-rnafusion_logo_light.png b/assets/nf-core-rnafusion_logo_light.png
index dee3db82069b61e9a5380d940355f47ff565057c..55f385411694bc192b1d6cf172a0c7defcd49d1a 100644
GIT binary patch
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diff --git a/conf/genomes.config b/conf/genomes.config
index 934041b2..cce08742 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -10,9 +10,9 @@
 params {
     genomes {
         'GRCh38' {
-            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh38_r${params.reference_release}.all.fa"
-            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh38_r${params.reference_release}.gtf"
-            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh38_r${params.reference_release}.cdna.all.fa.gz"
+            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh38.${ensembl_version}.all.fa"
+            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh38.${ensembl_version}.gtf"
+            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh38.${ensembl_version}.cdna.all.fa.gz"
             databases           = "${params.genomes_base}/databases"
             arriba_ref          = "${params.genomes_base}/arriba"
             ericscript_ref      = "${params.genomes_base}/ericscript/ericscript_db_homosapiens_ensembl84"
@@ -20,9 +20,9 @@ params {
             star_fusion_ref     = "${params.genomes_base}/star-fusion/ctat_genome_lib_build_dir"
         }
         'GRCh37' {
-            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.all.fa"
-            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.gtf"
-            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh37_r${params.reference_release}.cdna.all.fa.gz"
+            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh37.${ensembl_version}.all.fa"
+            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh37.${ensembl_version}.gtf"
+            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh37.${ensembl_version}.cdna.all.fa.gz"
             databases           = "${params.genomes_base}/databases"
             arriba_ref          = "${params.genomes_base}/arriba"
             ericscript_ref      = "${params.genomes_base}/ericscript/ericscript_db_homosapiens_ensembl73"
diff --git a/docs/images/nf-core-rnafusion_logo_dark.png b/docs/images/nf-core-rnafusion_logo_dark.png
index dee3db82069b61e9a5380d940355f47ff565057c..598d1e22bc313c63210e1738da0b746cc722e40a 100644
GIT binary patch
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diff --git a/docs/images/nf-core-rnafusion_logo_light.png b/docs/images/nf-core-rnafusion_logo_light.png
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literal 288
zcmaKn!EVAZ5Jd0(iYfQTPzk9AD^kHmL`q9kxd)CWS%D=(itV(`-**E&_qa1VJ8y-0
z(7UzWHwIh#TBG?`)zuoaM=2{~H&SY=@6J;(m(sk=N};CBQf<wP=D}9BHYJwxXS`GD
zhu9KofA$$wV#T#5>Xf{12_<hSwc6?ZbZAljlVMlb6XBZ<$!CI3$aMbfql-R_7%uJ*
z2)Re1_~p5F<aR{54wN!n0yEH0OVm-v@*i86*b31Ea*5M%ggZKaL2#L3!Gt$92A!T1
Ezej^vdH?_b

diff --git a/docs/usage.md b/docs/usage.md
index 07db3570..0498c039 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -46,7 +46,6 @@
     - [--fusioncatcher_ref](#--fusioncatcher_ref)
     - [--genome](#--genome)
     - [--gtf](#--gtf)
-    - [--reference_release](#--reference_release)
     - [--star_index](#--star_index)
     - [--star_fusion_ref](#--star_fusion_ref)
     - [--transcript](#--transcript)
@@ -189,7 +188,6 @@ sample,fastq_1,fastq_2
 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
 CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz
 CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz
-TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz,
 TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz,
 TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,
 TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,
@@ -543,15 +541,6 @@ Required annotation file.
 --gtf '<path to GTF annotation>'
 ```
 
-### --reference_release
-
-Ensembl version.
-
-```bash
-# ftp://ftp.ensembl.org/pub/release-97/fasta/homo_sapiens/
---reference_release '97'
-```
-
 ### --star_index
 
 If you prefer, you can specify the full path for `STAR` index when you run the pipeline.

From 641c6f5fc312ed45444e3f1dba4e5360adb46475 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 17 Jan 2022 16:46:17 +0100
Subject: [PATCH 095/359] input parameters fix

---
 nextflow.config      | 10 +++++--
 nextflow_schema.json | 65 ++++++++++++++++++++++++++++++++++++++++++++
 2 files changed, 72 insertions(+), 3 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 57076d35..538a5dd5 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -8,9 +8,7 @@
 
 // Global default params, used in configs
 params {
-    genome = 'GRCh38'
-    genomes_base = false
-    reference_release = '97'
+
 
     // Input options
     input                      = null
@@ -25,6 +23,12 @@ params {
     multiqc_title              = null
     max_multiqc_email_size     = '25.MB'
 
+    // Genome
+    genome                     = 'GRCh38'
+    genomes_base               = null
+    ensembl_version            = 97
+    read_length                = 100
+
     // Enable or disable tools
     all                        = false
     arriba                     = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index f602f89c..91437f09 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -41,6 +41,71 @@
                     "type": "string",
                     "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.",
                     "fa_icon": "fas fa-file-signature"
+                },
+                "genomes_base": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to reference folder"
+                },
+                "ensembl_version": {
+                    "type": "integer",
+                    "fa_icon": "far fa-file-code",
+                    "description": "ensembl version",
+                    "default": "97"
+                },
+                "read_length": {
+                    "type": "integer",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Read length",
+                    "default": "100"
+                },
+                "all": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run all references/analyses",
+                    "default": "false"
+                },
+                "arriba": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run arriba references/analyses",
+                    "default": "false"
+                },
+                "ericscript": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run ericscript references/analyses",
+                    "default": "false"
+                },
+                "fusioncatcher": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run fusioncatcher references/analyses",
+                    "default": "false"
+                },
+                "pizzly": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run pizzly references/analyses",
+                    "default": "false"
+                },
+                "squid": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run squid references/analyses",
+                    "default": "false"
+                },
+                "starindex": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run starindex references/analyses",
+                    "default": "false"
+                },
+                "starfusion": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run starfusion references/analyses",
+                    "default": "false"
                 }
             }
         },

From 0a1899d7f385cefa792445782405f2157ac69b7f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 19 Jan 2022 10:05:06 +0100
Subject: [PATCH 096/359] parameter fixes

---
 conf/base.config    | 12 ------------
 conf/genomes.config | 12 ++++++------
 2 files changed, 6 insertions(+), 18 deletions(-)

diff --git a/conf/base.config b/conf/base.config
index 63cef552..f2d66794 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -8,18 +8,6 @@
 ----------------------------------------------------------------------------------------
 */
 
-params {
-    versions {
-        arriba = '1.2.0'
-        ericscript = '0.5.5'
-        fusioncatcher = '1.20'
-        pizzly = '0.37.3'
-        squid = '1.5-star2.7.1a'
-        star_fusion = '1.9.1'
-        fusion_inspector = '2.3.1'
-    }
-}
-
 process {
 
     // TODO nf-core: Check the defaults for all processes
diff --git a/conf/genomes.config b/conf/genomes.config
index cce08742..54d84f66 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -10,9 +10,9 @@
 params {
     genomes {
         'GRCh38' {
-            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh38.${ensembl_version}.all.fa"
-            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh38.${ensembl_version}.gtf"
-            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh38.${ensembl_version}.cdna.all.fa.gz"
+            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
+            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
             databases           = "${params.genomes_base}/databases"
             arriba_ref          = "${params.genomes_base}/arriba"
             ericscript_ref      = "${params.genomes_base}/ericscript/ericscript_db_homosapiens_ensembl84"
@@ -20,9 +20,9 @@ params {
             star_fusion_ref     = "${params.genomes_base}/star-fusion/ctat_genome_lib_build_dir"
         }
         'GRCh37' {
-            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh37.${ensembl_version}.all.fa"
-            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh37.${ensembl_version}.gtf"
-            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh37.${ensembl_version}.cdna.all.fa.gz"
+            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.all.fa"
+            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.gtf"
+            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.cdna.all.fa.gz"
             databases           = "${params.genomes_base}/databases"
             arriba_ref          = "${params.genomes_base}/arriba"
             ericscript_ref      = "${params.genomes_base}/ericscript/ericscript_db_homosapiens_ensembl73"

From c6c63829f5294b1df1f9ca150c54531fcf2e8145 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 19 Jan 2022 11:28:54 +0100
Subject: [PATCH 097/359] Update README.md

Co-authored-by: Maxime U. Garcia <max.u.garcia@gmail.com>
---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 266a1e0b..b4bc5d33 100644
--- a/README.md
+++ b/README.md
@@ -62,7 +62,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
     <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
 
     ```console
-    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input samplesheet.csv --genome GRCh37
+    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input samplesheet.csv --genome GRCh38
     ```
 
 ## Documentation

From 6032a474468a4b4b670b97338d316134418c50fe Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 19 Jan 2022 11:29:01 +0100
Subject: [PATCH 098/359] Update containers/pizzly/Dockerfile

Co-authored-by: Maxime U. Garcia <max.u.garcia@gmail.com>
---
 containers/pizzly/Dockerfile | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/containers/pizzly/Dockerfile b/containers/pizzly/Dockerfile
index bc16bc85..0056bf9b 100644
--- a/containers/pizzly/Dockerfile
+++ b/containers/pizzly/Dockerfile
@@ -1,4 +1,4 @@
-containers/pizzly/Dockerfile FROM nfcore/base:1.9
+FROM nfcore/base:1.9
 
 LABEL authors="Martin Proks" \
     description="Docker image containing all requirements for nfcore/rnafusion pipeline"

From bef4cbaa145d630402438a9f4b18f4924afc11ed Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 19 Jan 2022 11:40:02 +0100
Subject: [PATCH 099/359] remove igenome

---
 conf/igenomes.config  | 432 ------------------------------------------
 conf/test.config      |   2 +-
 conf/test_full.config |   2 +-
 nextflow.config       |  10 +-
 nextflow_schema.json  |  15 --
 5 files changed, 3 insertions(+), 458 deletions(-)
 delete mode 100644 conf/igenomes.config

diff --git a/conf/igenomes.config b/conf/igenomes.config
deleted file mode 100644
index 855948de..00000000
--- a/conf/igenomes.config
+++ /dev/null
@@ -1,432 +0,0 @@
-/*
-========================================================================================
-    Nextflow config file for iGenomes paths
-========================================================================================
-    Defines reference genomes using iGenome paths.
-    Can be used by any config that customises the base path using:
-        $params.igenomes_base / --igenomes_base
-----------------------------------------------------------------------------------------
-*/
-
-params {
-    // illumina iGenomes reference file paths
-    genomes {
-        'GRCh37' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
-        }
-        'GRCh38' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-        }
-        'GRCm38' {
-            fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "1.87e9"
-            blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
-        }
-        'TAIR10' {
-            fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
-            mito_name   = "Mt"
-        }
-        'EB2' {
-            fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
-        }
-        'UMD3.1' {
-            fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'WBcel235' {
-            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
-            mito_name   = "MtDNA"
-            macs_gsize  = "9e7"
-        }
-        'CanFam3.1' {
-            fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'GRCz10' {
-            fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'BDGP6' {
-            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
-            mito_name   = "M"
-            macs_gsize  = "1.2e8"
-        }
-        'EquCab2' {
-            fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'EB1' {
-            fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
-        }
-        'Galgal4' {
-            fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'Gm01' {
-            fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
-        }
-        'Mmul_1' {
-            fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'IRGSP-1.0' {
-            fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
-            mito_name   = "Mt"
-        }
-        'CHIMP2.1.4' {
-            fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'Rnor_5.0' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'Rnor_6.0' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'R64-1-1' {
-            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-            macs_gsize  = "1.2e7"
-        }
-        'EF2' {
-            fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "1.21e7"
-        }
-        'Sbi1' {
-            fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
-        }
-        'Sscrofa10.2' {
-            fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'AGPv3' {
-            fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
-            mito_name   = "Mt"
-        }
-        'hg38' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-        }
-        'hg19' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
-        }
-        'mm10' {
-            fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "1.87e9"
-            blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
-        }
-        'bosTau8' {
-            fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-        }
-        'ce10' {
-            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "9e7"
-        }
-        'canFam3' {
-            fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'danRer10' {
-            fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "1.37e9"
-        }
-        'dm6' {
-            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "1.2e8"
-        }
-        'equCab2' {
-            fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'galGal4' {
-            fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'panTro4' {
-            fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'rn6' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-        }
-        'sacCer3' {
-            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
-            readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "1.2e7"
-        }
-        'susScr3' {
-            fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-    }
-}
diff --git a/conf/test.config b/conf/test.config
index d10f8c89..0dd30ff9 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -25,5 +25,5 @@ params {
     input  = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
 
     // Genome references
-    genome = 'R64-1-1'
+    genome = 'GRCh38'
 }
diff --git a/conf/test_full.config b/conf/test_full.config
index 260cb93f..ea387f70 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -20,5 +20,5 @@ params {
     input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv'
 
     // Genome references
-    genome = 'R64-1-1'
+    genome = 'GRCh38
 }
diff --git a/nextflow.config b/nextflow.config
index 538a5dd5..55fde253 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -15,8 +15,6 @@ params {
 
     // References
     genome                     = null
-    igenomes_base              = 's3://ngi-igenomes/igenomes'
-    igenomes_ignore            = false
 
     // MultiQC options
     multiqc_config             = null
@@ -28,6 +26,7 @@ params {
     genomes_base               = null
     ensembl_version            = 97
     read_length                = 100
+    genomes                    = [:]
 
     // Enable or disable tools
     all                        = false
@@ -133,13 +132,6 @@ profiles {
     test_full { includeConfig 'conf/test_full.config' }
 }
 
-// Load igenomes.config if required
-if (!params.igenomes_ignore) {
-    includeConfig 'conf/igenomes.config'
-} else {
-    params.genomes = [:]
-}
-
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container
 // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container.
 // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable.
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 91437f09..2d0fe4cb 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -129,21 +129,6 @@
                     "description": "Path to FASTA genome file.",
                     "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.",
                     "fa_icon": "far fa-file-code"
-                },
-                "igenomes_base": {
-                    "type": "string",
-                    "format": "directory-path",
-                    "description": "Directory / URL base for iGenomes references.",
-                    "default": "s3://ngi-igenomes/igenomes",
-                    "fa_icon": "fas fa-cloud-download-alt",
-                    "hidden": true
-                },
-                "igenomes_ignore": {
-                    "type": "boolean",
-                    "description": "Do not load the iGenomes reference config.",
-                    "fa_icon": "fas fa-ban",
-                    "hidden": true,
-                    "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
                 }
             }
         },

From 6cd2194b5429a241ac15970c6226acc2ca1fd8ed Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 19 Jan 2022 12:05:20 +0100
Subject: [PATCH 100/359] add link to fasta for test

---
 conf/test.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/test.config b/conf/test.config
index 0dd30ff9..fe67252e 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -23,6 +23,7 @@ params {
     // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
     // TODO nf-core: Give any required params for the test so that command line flags are not needed
     input  = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
+    fasta = 'https://github.com/nf-core/test-datasets/raw/sarek/reference/chr20_hg38/Homo_sapiens_assembly38_chr20.fasta'
 
     // Genome references
     genome = 'GRCh38'

From bcc586613549b8208252ad6c0133bac0b11f2b72 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 19 Jan 2022 18:16:33 +0100
Subject: [PATCH 101/359] remove args definition

---
 modules/local/arriba/detect/main.nf            | 3 +--
 modules/local/ericscript/detect/main.nf        | 2 +-
 modules/local/fusioncatcher/detect/main.nf     | 2 +-
 modules/local/fusioncatcher/download/main.nf   | 4 +---
 modules/local/pizzly/detect/main.nf            | 1 -
 modules/local/pizzly/download/main.nf          | 1 -
 modules/local/star/align/main.nf               | 1 -
 modules/local/star/genomegenerate/main.nf      | 1 -
 modules/local/starfusion/detect/main.nf        | 2 --
 modules/local/starfusion/download/main.nf      | 3 ++-
 modules/nf-core/modules/fastqc/main.nf         | 1 -
 modules/nf-core/modules/kallisto/index/main.nf | 1 -
 modules/nf-core/modules/multiqc/main.nf        | 1 -
 modules/nf-core/modules/samtools/faidx/main.nf | 1 -
 14 files changed, 6 insertions(+), 18 deletions(-)

diff --git a/modules/local/arriba/detect/main.nf b/modules/local/arriba/detect/main.nf
index c3460fba..a0353d50 100644
--- a/modules/local/arriba/detect/main.nf
+++ b/modules/local/arriba/detect/main.nf
@@ -25,8 +25,7 @@ process ARRIBA {
 
     script:
     def prefix    = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
+
     """
     STAR \\
         --genomeDir $index \\
diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
index 2b55726d..59660d39 100644
--- a/modules/local/ericscript/detect/main.nf
+++ b/modules/local/ericscript/detect/main.nf
@@ -22,8 +22,8 @@ process ERICSCRIPT {
     tuple val(meta), path "./tmp/${prefix}.ericscript.results.total.tsv"   , emit: fusions_total
 
     script:
-    def args = task.ext.args ?: ''
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+
     """
     ericscript.pl \\
         -db $reference \\
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 90712326..108f330d 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -23,8 +23,8 @@ process FUSIONCATCHER {
     path "versions.yml"                                                        , emit: versions
 
     script:
-    def args = task.ext.args ?: ''
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+
     """
     fusioncatcher.py \\
         -d $reference \\
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 56190c3a..3392f0c7 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -17,11 +17,9 @@ process FUSIONCATCHER_DOWNLOAD {
     path "versions.yml"     , emit: versions
 
     script:
-
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
     def human_version = "v102"
     def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.aa"
+
     """
     if wget --spider "$url" 2>/dev/null; then
         wget $args $url
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index f3d4dbf5..557f949a 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -26,7 +26,6 @@ process PIZZLY {
     tuple val(meta), path "${prefix}.pizzly.unfiltered.json", emit: fusions_unfiltered
 
     script:
-    def args = task.ext.args ?: ''
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     kallisto quant -t $task.cpus -i $index --fusion -o output $reads
diff --git a/modules/local/pizzly/download/main.nf b/modules/local/pizzly/download/main.nf
index ded6da00..dba09750 100644
--- a/modules/local/pizzly/download/main.nf
+++ b/modules/local/pizzly/download/main.nf
@@ -20,7 +20,6 @@ process PIZZLY_DOWNLOAD {
     path "index.idx"    , emit: reference
 
     script:
-    def args = task.ext.args ?: ''
     """
     kallisto index \\
         -i index.idx \\
diff --git a/modules/local/star/align/main.nf b/modules/local/star/align/main.nf
index 087414c3..de7af87a 100644
--- a/modules/local/star/align/main.nf
+++ b/modules/local/star/align/main.nf
@@ -33,7 +33,6 @@ process STAR_ALIGN {
     tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
 
     script:
-    def args = task.ext.args ?: ''
     def prefix          = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     def ignore_gtf      = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
     def seq_platform    = params.seq_platform ? "'PL:$params.seq_platform'" : ""
diff --git a/modules/local/star/genomegenerate/main.nf b/modules/local/star/genomegenerate/main.nf
index d113fc20..711ef7f1 100644
--- a/modules/local/star/genomegenerate/main.nf
+++ b/modules/local/star/genomegenerate/main.nf
@@ -22,7 +22,6 @@ process STAR_GENOMEGENERATE {
 
     script:
     def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
-    def args = task.ext.args ?: ''
     """
     mkdir star
     STAR \\
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 1b6c1afb..d32384a0 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -24,8 +24,6 @@ process STARFUSION {
 
     script:
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
     """
     STAR \\
         --genomeDir $index \\
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 47a6a7c8..59236c7b 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -20,6 +20,7 @@ process STARFUSION_DOWNLOAD {
     path "*"  , emit: reference
 
     script:
+    def binPath = if (workflow.containerEngine == "singularity" || workflow.containerEngine == "docker") ? "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/" : ""
     """
     export TMPDIR=/tmp
 
@@ -34,7 +35,7 @@ process STARFUSION_DOWNLOAD {
 
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
 
-    /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl \\
+    ${binPath}prep_genome.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --annot_filter_rule AnnotFilterRule.pm \\
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
index d250eca0..f278a5df 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -16,7 +16,6 @@ process FASTQC {
     path  "versions.yml"           , emit: versions
 
     script:
-    def args = task.ext.args ?: ''
     // Add soft-links to original FastQs for consistent naming in pipeline
     def prefix = task.ext.prefix ?: "${meta.id}"
     if (meta.single_end) {
diff --git a/modules/nf-core/modules/kallisto/index/main.nf b/modules/nf-core/modules/kallisto/index/main.nf
index 4dc9c6d0..e6ebaf7a 100644
--- a/modules/nf-core/modules/kallisto/index/main.nf
+++ b/modules/nf-core/modules/kallisto/index/main.nf
@@ -15,7 +15,6 @@ process KALLISTO_INDEX {
     path "versions.yml" , emit: versions
 
     script:
-    def args = task.ext.args ?: ''
     """
     kallisto \\
         index \\
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index 3dceb162..fb90b1b1 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -16,7 +16,6 @@ process MULTIQC {
     path "versions.yml"        , emit: versions
 
     script:
-    def args = task.ext.args ?: ''
     """
     multiqc -f $args .
 
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf
index 58c445d8..41270a39 100644
--- a/modules/nf-core/modules/samtools/faidx/main.nf
+++ b/modules/nf-core/modules/samtools/faidx/main.nf
@@ -15,7 +15,6 @@ process SAMTOOLS_FAIDX {
     path "versions.yml"            , emit: versions
 
     script:
-    def args = task.ext.args ?: ''
     """
     samtools \\
         faidx \\

From f354fbea0e2306e660e3bf04680b1219c4f071d7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 20 Jan 2022 09:18:13 +0100
Subject: [PATCH 102/359] path to prep_genome_lib.pl

---
 modules/local/starfusion/download/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 59236c7b..c6d2e85f 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -20,7 +20,7 @@ process STARFUSION_DOWNLOAD {
     path "*"  , emit: reference
 
     script:
-    def binPath = if (workflow.containerEngine == "singularity" || workflow.containerEngine == "docker") ? "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/" : ""
+    def binPath = { workflow.containerEngine == "singularity" || workflow.containerEngine == "docker" ? "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl" : "prep_genome_lib.pl" }
     """
     export TMPDIR=/tmp
 
@@ -35,7 +35,7 @@ process STARFUSION_DOWNLOAD {
 
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
 
-    ${binPath}prep_genome.pl \\
+    $binPath \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --annot_filter_rule AnnotFilterRule.pm \\

From 65f80543af3eb90f701841f8c1555ba2e4fe6fa0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 20 Jan 2022 09:41:36 +0100
Subject: [PATCH 103/359] remove igenomes

---
 conf/igenomes.config | 432 -------------------------------------------
 nextflow.config      |   2 +-
 2 files changed, 1 insertion(+), 433 deletions(-)
 delete mode 100644 conf/igenomes.config

diff --git a/conf/igenomes.config b/conf/igenomes.config
deleted file mode 100644
index 6ff960c8..00000000
--- a/conf/igenomes.config
+++ /dev/null
@@ -1,432 +0,0 @@
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    Nextflow config file for iGenomes paths
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    Defines reference genomes using iGenome paths.
-    Can be used by any config that customises the base path using:
-        $params.igenomes_base / --igenomes_base
-----------------------------------------------------------------------------------------
-*/
-
-params {
-    // illumina iGenomes reference file paths
-    genomes {
-        'GRCh37' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
-        }
-        'GRCh38' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-        }
-        'GRCm38' {
-            fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "1.87e9"
-            blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
-        }
-        'TAIR10' {
-            fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
-            mito_name   = "Mt"
-        }
-        'EB2' {
-            fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
-        }
-        'UMD3.1' {
-            fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'WBcel235' {
-            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
-            mito_name   = "MtDNA"
-            macs_gsize  = "9e7"
-        }
-        'CanFam3.1' {
-            fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'GRCz10' {
-            fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'BDGP6' {
-            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
-            mito_name   = "M"
-            macs_gsize  = "1.2e8"
-        }
-        'EquCab2' {
-            fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'EB1' {
-            fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
-        }
-        'Galgal4' {
-            fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'Gm01' {
-            fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
-        }
-        'Mmul_1' {
-            fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'IRGSP-1.0' {
-            fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
-            mito_name   = "Mt"
-        }
-        'CHIMP2.1.4' {
-            fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'Rnor_5.0' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'Rnor_6.0' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-        }
-        'R64-1-1' {
-            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-            macs_gsize  = "1.2e7"
-        }
-        'EF2' {
-            fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "1.21e7"
-        }
-        'Sbi1' {
-            fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
-        }
-        'Sscrofa10.2' {
-            fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
-            mito_name   = "MT"
-        }
-        'AGPv3' {
-            fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
-            mito_name   = "Mt"
-        }
-        'hg38' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-        }
-        'hg19' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
-        }
-        'mm10' {
-            fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "1.87e9"
-            blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
-        }
-        'bosTau8' {
-            fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-        }
-        'ce10' {
-            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "9e7"
-        }
-        'canFam3' {
-            fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'danRer10' {
-            fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "1.37e9"
-        }
-        'dm6' {
-            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "1.2e8"
-        }
-        'equCab2' {
-            fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'galGal4' {
-            fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'panTro4' {
-            fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-        'rn6' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-        }
-        'sacCer3' {
-            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
-            readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "1.2e7"
-        }
-        'susScr3' {
-            fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
-            bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
-            mito_name   = "chrM"
-        }
-    }
-}
diff --git a/nextflow.config b/nextflow.config
index 8f60174c..d64d1536 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -21,7 +21,7 @@ params {
 
     // Genome
     genome                     = 'GRCh38'
-    genomes                    = null
+    genomes                    = [:]
     genomes_base               = null
     ensembl_version            = 97
     read_length                = 100

From adce80d7c24fef19ee9aa0772be3a810bbcbb5f7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 20 Jan 2022 11:51:01 +0100
Subject: [PATCH 104/359] define args in modules is necessary

---
 modules/local/arriba/detect/main.nf            | 3 ++-
 modules/local/ericscript/detect/main.nf        | 2 +-
 modules/local/fusioncatcher/detect/main.nf     | 2 +-
 modules/local/fusioncatcher/download/main.nf   | 4 +++-
 modules/local/pizzly/detect/main.nf            | 1 +
 modules/local/pizzly/download/main.nf          | 1 +
 modules/local/star/align/main.nf               | 1 +
 modules/local/star/genomegenerate/main.nf      | 1 +
 modules/local/starfusion/detect/main.nf        | 2 ++
 modules/nf-core/modules/fastqc/main.nf         | 1 +
 modules/nf-core/modules/kallisto/index/main.nf | 1 +
 modules/nf-core/modules/multiqc/main.nf        | 1 +
 modules/nf-core/modules/samtools/faidx/main.nf | 1 +
 13 files changed, 17 insertions(+), 4 deletions(-)

diff --git a/modules/local/arriba/detect/main.nf b/modules/local/arriba/detect/main.nf
index a0353d50..c3460fba 100644
--- a/modules/local/arriba/detect/main.nf
+++ b/modules/local/arriba/detect/main.nf
@@ -25,7 +25,8 @@ process ARRIBA {
 
     script:
     def prefix    = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
     """
     STAR \\
         --genomeDir $index \\
diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
index 59660d39..2b55726d 100644
--- a/modules/local/ericscript/detect/main.nf
+++ b/modules/local/ericscript/detect/main.nf
@@ -22,8 +22,8 @@ process ERICSCRIPT {
     tuple val(meta), path "./tmp/${prefix}.ericscript.results.total.tsv"   , emit: fusions_total
 
     script:
+    def args = task.ext.args ?: ''
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-
     """
     ericscript.pl \\
         -db $reference \\
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 108f330d..90712326 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -23,8 +23,8 @@ process FUSIONCATCHER {
     path "versions.yml"                                                        , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-
     """
     fusioncatcher.py \\
         -d $reference \\
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 3392f0c7..56190c3a 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -17,9 +17,11 @@ process FUSIONCATCHER_DOWNLOAD {
     path "versions.yml"     , emit: versions
 
     script:
+
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
     def human_version = "v102"
     def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.aa"
-
     """
     if wget --spider "$url" 2>/dev/null; then
         wget $args $url
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index 557f949a..f3d4dbf5 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -26,6 +26,7 @@ process PIZZLY {
     tuple val(meta), path "${prefix}.pizzly.unfiltered.json", emit: fusions_unfiltered
 
     script:
+    def args = task.ext.args ?: ''
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     """
     kallisto quant -t $task.cpus -i $index --fusion -o output $reads
diff --git a/modules/local/pizzly/download/main.nf b/modules/local/pizzly/download/main.nf
index dba09750..ded6da00 100644
--- a/modules/local/pizzly/download/main.nf
+++ b/modules/local/pizzly/download/main.nf
@@ -20,6 +20,7 @@ process PIZZLY_DOWNLOAD {
     path "index.idx"    , emit: reference
 
     script:
+    def args = task.ext.args ?: ''
     """
     kallisto index \\
         -i index.idx \\
diff --git a/modules/local/star/align/main.nf b/modules/local/star/align/main.nf
index de7af87a..087414c3 100644
--- a/modules/local/star/align/main.nf
+++ b/modules/local/star/align/main.nf
@@ -33,6 +33,7 @@ process STAR_ALIGN {
     tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
 
     script:
+    def args = task.ext.args ?: ''
     def prefix          = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
     def ignore_gtf      = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
     def seq_platform    = params.seq_platform ? "'PL:$params.seq_platform'" : ""
diff --git a/modules/local/star/genomegenerate/main.nf b/modules/local/star/genomegenerate/main.nf
index 711ef7f1..d113fc20 100644
--- a/modules/local/star/genomegenerate/main.nf
+++ b/modules/local/star/genomegenerate/main.nf
@@ -22,6 +22,7 @@ process STAR_GENOMEGENERATE {
 
     script:
     def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
+    def args = task.ext.args ?: ''
     """
     mkdir star
     STAR \\
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index d32384a0..1b6c1afb 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -24,6 +24,8 @@ process STARFUSION {
 
     script:
     def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
     """
     STAR \\
         --genomeDir $index \\
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
index f278a5df..d250eca0 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -16,6 +16,7 @@ process FASTQC {
     path  "versions.yml"           , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     // Add soft-links to original FastQs for consistent naming in pipeline
     def prefix = task.ext.prefix ?: "${meta.id}"
     if (meta.single_end) {
diff --git a/modules/nf-core/modules/kallisto/index/main.nf b/modules/nf-core/modules/kallisto/index/main.nf
index e6ebaf7a..4dc9c6d0 100644
--- a/modules/nf-core/modules/kallisto/index/main.nf
+++ b/modules/nf-core/modules/kallisto/index/main.nf
@@ -15,6 +15,7 @@ process KALLISTO_INDEX {
     path "versions.yml" , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     """
     kallisto \\
         index \\
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index fb90b1b1..3dceb162 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -16,6 +16,7 @@ process MULTIQC {
     path "versions.yml"        , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     """
     multiqc -f $args .
 
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf
index 41270a39..58c445d8 100644
--- a/modules/nf-core/modules/samtools/faidx/main.nf
+++ b/modules/nf-core/modules/samtools/faidx/main.nf
@@ -15,6 +15,7 @@ process SAMTOOLS_FAIDX {
     path "versions.yml"            , emit: versions
 
     script:
+    def args = task.ext.args ?: ''
     """
     samtools \\
         faidx \\

From 4803137e0cbbbe17c489c14e924febf39c40b99e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 20 Jan 2022 12:16:41 +0100
Subject: [PATCH 105/359] gtf to replace chr.gtf in ensembl download

---
 modules/local/ensembl/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 516b07db..872ea3c1 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -18,7 +18,7 @@ process ENSEMBL_DOWNLOAD {
     output:
     path "versions.yml"                                                   , emit: versions
     path "Homo_sapiens.${params.genome}.${ensembl_version}.all.fa"        , emit: fasta
-    path "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"       , emit: gtf
+    path "Homo_sapiens.${params.genome}.${ensembl_version}.gtf"           , emit: gtf
     path "Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz", emit: transcript
 
     script:
@@ -26,7 +26,7 @@ process ENSEMBL_DOWNLOAD {
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
 
-    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
+    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz
 
     gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa

From d834cf59acfd15e98c647fe2bd2f97173f5364a7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 20 Jan 2022 12:19:26 +0100
Subject: [PATCH 106/359] gtf to replace chr.gtf in ensembl download, part2

---
 conf/genomes.config           | 2 +-
 modules/local/ensembl/main.nf | 4 ++--
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index 869bf065..6f5c4850 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -12,7 +12,7 @@ params {
     genomes {
         'GRCh38' {
             fasta         = "${params.genomes_base}/Homo_sapiens.GRCh38.all.fa"
-            gtf           = "${params.genomes_base}/Homo_sapiens.GRCh38.chr.gtf"
+            gtf           = "${params.genomes_base}/Homo_sapiens.GRCh38.gtf"
             transcript    = "${params.genomes_base}/Homo_sapiens.GRCh38.cdna.all.fa.gz"
             star          = "${params.genomes_base}/star"
             arriba        = "${params.genomes_base}/arriba"
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 872ea3c1..ff3f7a6f 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -30,7 +30,7 @@ process ENSEMBL_DOWNLOAD {
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz
 
     gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa
-    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
+    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
 
     echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """
@@ -38,7 +38,7 @@ process ENSEMBL_DOWNLOAD {
     stub:
     """
     touch "Homo_sapiens.${params.genome}.${ensembl_version}.all.fa"
-    touch "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"
+    touch "Homo_sapiens.${params.genome}.${ensembl_version}.gtf"
     touch "Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz"
 
     touch versions.yml

From 3877ee268f7f391df6f6939c3b861145d0b29b33 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 24 Jan 2022 15:11:59 +0100
Subject: [PATCH 107/359] merging dev and dsl2, deleting logo

---
 assets/nf-core-rnafusion_logo.png | Bin 18138 -> 0 bytes
 1 file changed, 0 insertions(+), 0 deletions(-)
 delete mode 100644 assets/nf-core-rnafusion_logo.png

diff --git a/assets/nf-core-rnafusion_logo.png b/assets/nf-core-rnafusion_logo.png
deleted file mode 100644
index e3b18bf3d2adae1b7f0b3ca752fe50f0fa9665cc..0000000000000000000000000000000000000000
GIT binary patch
literal 0
HcmV?d00001

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zxi{Fbiw)duuxcp*|KaE7kNsT$L|ve_>eX8X)z@>uA{byBUoUL?-kI8_wXZD~aT!tE
zf9hx<{T)M6lnoK$857>4+=`Og9XEpMyoX-#q%N73IFyMf5|9BMos^MeFZyc|dS+C3
z4Thv%%s!zvCKL}7dl%T82>=%;EXdIJXBQK*DWR9Cmol-sxgh6hFoYbEPr?m}A&Q&y
zG7tf>LP>00vibi19zMdZglhT<3VZ5EWcbDZdko2cCK4f8H^+ZJ@C=<=WHVdB@A<#y
znH+NM4S;>jgqZO)y3aB<?238*d(;Z}qd3iN!yNV`6FMC&n))g0>20?EeGTV<dL7UI
z4i-ED^UJ{ZjrX*iaQye&m4i<fu{*HWj!|C^ivo8JJouyn3ua`2b3iC!(VcmP`@e^f
zfakd|YwF}qvGx9UF0MNOdW`;eDkhW7oZyqa)H?rr+)KR4k|*q9*KjKk|9k#R{Pj~r
Yfm!PakM>XSuo}o4X=SM@36tRe4=(oKumAu6


From bdabfb3f86367baed029f02550208dfc903ed208 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 24 Jan 2022 15:15:07 +0100
Subject: [PATCH 108/359] linting fix for template

---
 assets/email_template.html | 10 +++++-----
 1 file changed, 5 insertions(+), 5 deletions(-)

diff --git a/assets/email_template.html b/assets/email_template.html
index 89c6245a..efcd0eaa 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -1,11 +1,11 @@
 <html>
 <head>
-    <meta charset="utf-8">
-    <meta http-equiv="X-UA-Compatible" content="IE=edge">
-    <meta name="viewport" content="width=device-width, initial-scale=1">
+  <meta charset="utf-8">
+  <meta http-equiv="X-UA-Compatible" content="IE=edge">
+  <meta name="viewport" content="width=device-width, initial-scale=1">
 
-    <meta name="description" content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community.">
-    <title>nf-core/rnafusion Pipeline Report</title>
+  <meta name="description" content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community.">
+  <title>nf-core/rnafusion Pipeline Report</title>
 </head>
 <body>
 <div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto;">

From e4c6f59ad4449eeb3b3559ac600c266eafed7e8a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 24 Jan 2022 15:49:06 +0100
Subject: [PATCH 109/359] comment out parameters in modules.config not
 currently in use + remove publishDir definition in the modules

---
 conf/modules.config                          | 32 ++++++++++++--------
 modules/local/arriba/detect/main.nf          |  3 --
 modules/local/arriba/download/main.nf        |  3 --
 modules/local/ensembl/main.nf                |  3 --
 modules/local/ericscript/detect/main.nf      |  3 --
 modules/local/ericscript/download/main.nf    |  3 --
 modules/local/fusioncatcher/detect/main.nf   |  3 --
 modules/local/fusioncatcher/download/main.nf |  3 --
 modules/local/pizzly/detect/main.nf          |  3 --
 modules/local/pizzly/download/main.nf        |  3 --
 modules/local/star/align/main.nf             |  3 --
 modules/local/star/genomegenerate/main.nf    |  3 --
 modules/local/starfusion/detect/main.nf      |  3 --
 modules/local/starfusion/download/main.nf    |  3 --
 14 files changed, 20 insertions(+), 51 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 8cc86aab..2194e478 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -39,21 +39,24 @@ process {
         ]
     }
 
-    withName: ARRIBA {
-        ext.args  = "--outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
-        ext.args2 = "-b references/${params.genome}/arriba/blacklist_hg38_GRCh38_v2.1.0.tsv.gz -k references/${params.genome}/arriba/known_fusions_hg38_GRCh38_v2.1.0.tsv.gz -p references/${params.genome}/arriba/protein_domains_hg38_GRCh38_v2.1.0.gff3"
-    }
 
     withName: FUSIONCATCHER_DOWNLOAD {
         ext.args = "--continue --tries=42"
     }
 
-    withName: PIZZLY {
-        ext.args = = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
+    withName: KALLISTO_INDEX {
+        ext.args = '-k 31'
+    }
+
+    withName: STARFUSION_DOWNLOAD {
+        cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
+        memory = { check_max( 100.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 2.d  * task.attempt, 'time'    ) }
     }
 
-    withName: PIZZLY_DOWNLOAD {
-        ext.args = = "-k 31"
+/*
+    withName: PIZZLY {
+        ext.args = = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
     }
 
     withName: STARFUSION {
@@ -61,9 +64,14 @@ process {
         ext.args2 = "--examine_coding_effect -J Chimeric.out.junction"
     }
 
-    withName: STARFUSION_DOWNLOAD {
-        cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
-        memory = { check_max( 100.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 2.d  * task.attempt, 'time'    ) }
+    withName: ARRIBA {
+        ext.args  = "--outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
+        ext.args2 = "-b references/${params.genome}/arriba/blacklist_hg38_GRCh38_v2.1.0.tsv.gz -k references/${params.genome}/arriba/known_fusions_hg38_GRCh38_v2.1.0.tsv.gz -p references/${params.genome}/arriba/protein_domains_hg38_GRCh38_v2.1.0.gff3"
     }
+
+*/
+
+
 }
+
+
diff --git a/modules/local/arriba/detect/main.nf b/modules/local/arriba/detect/main.nf
index c3460fba..a543c82f 100644
--- a/modules/local/arriba/detect/main.nf
+++ b/modules/local/arriba/detect/main.nf
@@ -1,9 +1,6 @@
 process ARRIBA {
     tag "$meta.id"
     label 'process_medium'
-    publishDir "${params.outdir}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     conda (params.enable_conda ? "bioconda::arriba=2.1.0" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/arriba/download/main.nf b/modules/local/arriba/download/main.nf
index 39afce76..d7620345 100644
--- a/modules/local/arriba/download/main.nf
+++ b/modules/local/arriba/download/main.nf
@@ -1,9 +1,6 @@
 process ARRIBA_DOWNLOAD {
     tag "arriba"
     label 'process_low'
-    publishDir "${params.genomes_base}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index ff3f7a6f..2521a97b 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -1,9 +1,6 @@
 process ENSEMBL_DOWNLOAD {
     tag "ensembl $ensembl_version"
     label 'process_low'
-    publishDir "${params.genomes_base}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
index 2b55726d..dc77ff42 100644
--- a/modules/local/ericscript/detect/main.nf
+++ b/modules/local/ericscript/detect/main.nf
@@ -1,9 +1,6 @@
 process ERICSCRIPT {
     tag "eriscript"
     label 'process_medium'
-    publishDir "${params.outdir}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? "bioconda::ericscript=0.5.5" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/ericscript/download/main.nf b/modules/local/ericscript/download/main.nf
index 5441b6bc..c72bc69e 100644
--- a/modules/local/ericscript/download/main.nf
+++ b/modules/local/ericscript/download/main.nf
@@ -1,9 +1,6 @@
 process ERICSCRIPT_DOWNLOAD {
     tag "eriscript"
     label 'process_low'
-    publishDir "${params.genomes_base}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 90712326..15a552ef 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -1,9 +1,6 @@
 process FUSIONCATCHER {
     tag "$meta.id"
     label 'process_high'
-    publishDir "${params.outdir}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 56190c3a..65a3db52 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -1,9 +1,6 @@
 process FUSIONCATCHER_DOWNLOAD {
     tag 'fusioncatcher'
     label 'process_medium'
-    publishDir "${params.genomes_base}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index f3d4dbf5..790987ba 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -1,9 +1,6 @@
 process PIZZLY {
     tag "pizzly"
     label 'process_medium'
-    publishDir "${params.outdir}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2 bioconda::pizzly==0.37.3" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/pizzly/download/main.nf b/modules/local/pizzly/download/main.nf
index ded6da00..d3be739b 100644
--- a/modules/local/pizzly/download/main.nf
+++ b/modules/local/pizzly/download/main.nf
@@ -1,9 +1,6 @@
 process PIZZLY_DOWNLOAD {
     tag "pizzly"
     label 'process_medium'
-    publishDir "${params.genomes_base}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/star/align/main.nf b/modules/local/star/align/main.nf
index 087414c3..49e7d8ae 100644
--- a/modules/local/star/align/main.nf
+++ b/modules/local/star/align/main.nf
@@ -1,9 +1,6 @@
 process STAR_ALIGN {
     tag "$meta.id"
     label 'process_high'
-    publishDir "${params.outdir}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     // Note: 2.7X indices incompatible with AWS iGenomes.
     conda (params.enable_conda ? 'bioconda::star=2.7.8a' : null)
diff --git a/modules/local/star/genomegenerate/main.nf b/modules/local/star/genomegenerate/main.nf
index d113fc20..9f5b7786 100644
--- a/modules/local/star/genomegenerate/main.nf
+++ b/modules/local/star/genomegenerate/main.nf
@@ -1,9 +1,6 @@
 process STAR_GENOMEGENERATE {
     tag "$fasta"
     label 'process_high'
-    publishDir "${params.genomes_base}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 1b6c1afb..ffbf632f 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -1,9 +1,6 @@
 process STARFUSION {
     tag "$meta.id"
     label 'process_high'
-    publishDir "${params.outdir}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publishDir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
 
     conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index c6d2e85f..c6bda87f 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -1,9 +1,6 @@
 process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
     label 'process_high'
-    publishDir "${params.genomes_base}",
-        mode: params.publishDir_mode,
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
 
     conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {

From 7d2f3491555f31e193b62e81a4fb330a8cf2c0b9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 24 Jan 2022 16:19:25 +0100
Subject: [PATCH 110/359] markdown linting

---
 README.md     |  2 +-
 docs/usage.md | 11 ++++++-----
 2 files changed, 7 insertions(+), 6 deletions(-)

diff --git a/README.md b/README.md
index 17280ca9..926e8196 100644
--- a/README.md
+++ b/README.md
@@ -72,7 +72,7 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline [usag
 nf-core/rnafusion was originally written by Martin Proks, @praveenraj2018
 
 We thank the following people for their extensive assistance in the development of this pipeline:
-- Annick Renevey (@rannick)
+* Annick Renevey (@rannick)
 
 ## Contributions and Support
 
diff --git a/docs/usage.md b/docs/usage.md
index 8beb7693..a738fbb1 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -101,6 +101,7 @@ nextflow run nf-core/rnafusion/download-references.nf \
 --fusion_report \
 --outdir <PATH>
 --cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+
 ```
 
 ### Download and build CTAT
@@ -327,9 +328,9 @@ For the ultimate flexibility, we have implemented and are using Nextflow DSL2 mo
 
 The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of:
 
-* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file.
+- Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file.
 
-* An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option.
+- An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option.
 
 The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments.
 
@@ -341,9 +342,9 @@ Say for example we want to append an additional, non-mandatory parameter (i.e. `
 
 As you will see in the example below, we have:
 
-* appended `--outFilterMismatchNmax 16` to the default `args` used by the module.
-* changed the default `publishDir` value to where the files will eventually be published in the main results directory.
-* appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`.
+- appended `--outFilterMismatchNmax 16` to the default `args` used by the module.
+- changed the default `publishDir` value to where the files will eventually be published in the main results directory.
+- appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`.
 
 ```nextflow
 params {

From 94735313e45a53a0cd2d2cfee63fcad437deb921 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 24 Jan 2022 16:32:16 +0100
Subject: [PATCH 111/359] markdown linting

---
 README.md     |  1 +
 docs/usage.md | 11 +++++------
 2 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/README.md b/README.md
index 926e8196..6384f70b 100644
--- a/README.md
+++ b/README.md
@@ -72,6 +72,7 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline [usag
 nf-core/rnafusion was originally written by Martin Proks, @praveenraj2018
 
 We thank the following people for their extensive assistance in the development of this pipeline:
+
 * Annick Renevey (@rannick)
 
 ## Contributions and Support
diff --git a/docs/usage.md b/docs/usage.md
index a738fbb1..ab6ae1b1 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -69,7 +69,7 @@ The rnafusion pipeline needs references for the fusion detection tools, so downl
 Downloading references manually is a tedious long process.
 To make the pipeline easier to work with, we provide a script to download all necessary references for fusion detection tools.
 
-> **TL;DR:** Make sure to download the correct references for your need!
+**TL;DR:** Make sure to download the correct references for your need!
 
 ### Download all references
 
@@ -79,8 +79,9 @@ nextflow run nf-core/rnafusion \
 --outdir <PATH> \
 ```
 
-# Example of downloading references for specific tool
+### Example of downloading references for specific tool
 
+```bash
 nextflow run nf-core/rnafusion/download-references.nf \
 --arriba \
 --outdir <PATH>
@@ -100,11 +101,9 @@ nextflow run nf-core/rnafusion/download-references.nf \
 nextflow run nf-core/rnafusion/download-references.nf \
 --fusion_report \
 --outdir <PATH>
---cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
-
 ```
 
-### Download and build CTAT
+## Download and build CTAT
 
 ```bash
 nextflow run nf-core/rnafusion/build-ctat.nf \
@@ -114,7 +113,7 @@ nextflow run nf-core/rnafusion/build-ctat.nf \
 --gtf <PATH>/<GTF>
 ```
 
-### Download GRCh37 references
+## Download GRCh37 references
 
 ```bash
 # GRCh38 genome assembly is used by default.

From 9926a75a726dcd2d46ea657de0d3f49c6a08c2da Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 24 Jan 2022 16:44:35 +0100
Subject: [PATCH 112/359] minor changes

---
 bin/scrape_software_versions.py | 36 ---------------------------------
 conf/genomes.config             |  2 +-
 docs/usage.md                   |  2 +-
 3 files changed, 2 insertions(+), 38 deletions(-)
 delete mode 100644 bin/scrape_software_versions.py

diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py
deleted file mode 100644
index ee132097..00000000
--- a/bin/scrape_software_versions.py
+++ /dev/null
@@ -1,36 +0,0 @@
-#!/usr/bin/env python
-from __future__ import print_function
-import os
-
-results = {}
-version_files = [x for x in os.listdir(".") if x.endswith(".version.txt")]
-for version_file in version_files:
-
-    software = version_file.replace(".version.txt", "")
-    if software == "pipeline":
-        software = "nf-core/rnafusion"
-
-    with open(version_file) as fin:
-        version = fin.read().strip()
-    results[software] = version
-
-# Dump to YAML
-print(
-    """
-id: 'software_versions'
-section_name: 'nf-core/rnafusion Software Versions'
-section_href: 'https://github.com/nf-core/rnafusion'
-plot_type: 'html'
-description: 'are collected at run time from the software output.'
-data: |
-    <dl class="dl-horizontal">
-"""
-)
-for k, v in sorted(results.items()):
-    print("        <dt>{}</dt><dd><samp>{}</samp></dd>".format(k, v))
-print("    </dl>")
-
-# Write out as tsv file:
-with open("software_versions.tsv", "w") as f:
-    for k, v in sorted(results.items()):
-        f.write("{}\t{}\n".format(k, v))
diff --git a/conf/genomes.config b/conf/genomes.config
index deaf8e03..e653f068 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -1,6 +1,6 @@
 /*
  * -------------------------------------------------
- *  Nextflow config file for reference genomes
+ *  Nextflow config file for reference genome
  * -------------------------------------------------
  * These references have to be build manually due
  * to ERCC spike-ins.
diff --git a/docs/usage.md b/docs/usage.md
index ab6ae1b1..6ce66675 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -69,7 +69,7 @@ The rnafusion pipeline needs references for the fusion detection tools, so downl
 Downloading references manually is a tedious long process.
 To make the pipeline easier to work with, we provide a script to download all necessary references for fusion detection tools.
 
-**TL;DR:** Make sure to download the correct references for your need!
+> **TL;DR:** Make sure to download the correct references for your need!
 
 ### Download all references
 

From 25a6d0ea4db6ee73721265c4f1da4740573994d3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Feb 2022 15:20:24 +0100
Subject: [PATCH 113/359] update nf-core modules and prepare arguments for path
 to refernece

---
 conf/genomes.config                           | 44 ++++++++++---------
 conf/modules.config                           | 21 +++++++++
 modules.json                                  | 19 ++++----
 .../custom/dumpsoftwareversions/main.nf       |  3 ++
 modules/nf-core/modules/fastqc/main.nf        |  3 ++
 .../nf-core/modules/kallisto/index/main.nf    |  3 ++
 modules/nf-core/modules/multiqc/main.nf       |  3 ++
 .../nf-core/modules/samtools/faidx/main.nf    |  3 ++
 8 files changed, 71 insertions(+), 28 deletions(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index e653f068..251a4a21 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -11,28 +11,32 @@
 params {
     genomes {
         'GRCh38' {
-            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
-            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
-            databases           = "${params.genomes_base}/databases"
-            star                = "${params.genomes_base}/star"
-            arriba              = "${params.genomes_base}/arriba"
-            ericscript          = "${params.genomes_base}/ericscript"
-            fusioncatcher       = "${params.genomes_base}/fusioncatcher"
-            pizzly              = "${params.genomes_base}/pizzly"
-            starfusion          = "${params.genomes_base}/starfusion"
+            fasta                         = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
+            gtf                           = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+            transcript                    = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
+            arriba_ref                    = "${params.genomes_base}/arriba"
+            ensembl_ref                   = "${params.genomes_base}/ensembl"
+            ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
+            fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
+            pizzly_ref                    = "${params.genomes_base}/pizzly"
+            squid_ref                     = "${params.genomes_base}/squid"
+            starfusion_ref                = "${params.genomes_base}/starfusion"
+            starindex_ref                 = "${params.genomes_base}/starindex
+
         }
         'GRCh37' {
-            fasta               = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.all.fa"
-            gtf                 = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.gtf"
-            transcript          = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.cdna.all.fa.gz"
-            databases           = "${params.genomes_base}/databases"
-            star                = "${params.genomes_base}/star"
-            arriba              = "${params.genomes_base}/arriba"
-            ericscript          = "${params.genomes_base}/ericscript"
-            fusioncatcher       = "${params.genomes_base}/fusioncatcher"
-            pizzly              = "${params.genomes_base}/pizzly"
-            starfusion          = "${params.genomes_base}/starfusion"
+            fasta                         = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.all.fa"
+            gtf                           = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.gtf"
+            transcript                    = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.cdna.all.fa.gz"
+            arriba_ref                    = "${params.genomes_base}/arriba"
+            ensembl_ref                   = "${params.genomes_base}/ensembl"
+            ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
+            fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
+            pizzly_ref                    = "${params.genomes_base}/pizzly"
+            squid_ref                     = "${params.genomes_base}/squid"
+            starfusion_ref                = "${params.genomes_base}/starfusion"
+            starindex_ref                 = "${params.genomes_base}/starindex
+
         }
     }
 }
diff --git a/conf/modules.config b/conf/modules.config
index 2194e478..32ce380d 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -39,6 +39,13 @@ process {
         ]
     }
 
+    withName: ENSEMBL_DOWNLOAD {
+        publishDir = [
+            path: { "${params.genomes_base}/ensembl" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
 
     withName: FUSIONCATCHER_DOWNLOAD {
         ext.args = "--continue --tries=42"
@@ -52,8 +59,22 @@ process {
         cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
         memory = { check_max( 100.GB * task.attempt, 'memory'  ) }
         time   = { check_max( 2.d  * task.attempt, 'time'    ) }
+        publishDir = [
+            path: { "${params.genomes_base}/starfusion" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
     }
 
+    withName: ERICSCRIPT_DOWNLOAD {
+    publishDir = [
+        path: { "${params.genomes_base}/ericscript" },
+        mode: 'copy',
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+    ]
+}
+
+
 /*
     withName: PIZZLY {
         ext.args = = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
diff --git a/modules.json b/modules.json
index 90fd1608..166e7244 100644
--- a/modules.json
+++ b/modules.json
@@ -4,20 +4,23 @@
     "repos": {
         "nf-core/modules": {
             "custom/dumpsoftwareversions": {
-                "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41"
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
             "fastqc": {
-                "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961"
-            },
-            "multiqc": {
-                "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41"
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
             "kallisto/index": {
-                "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41"
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+            },
+            "multiqc": {
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
             "samtools/faidx": {
-                "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683"
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+            },
+            "star/genomegenerate": {
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
index 934bb467..327d5100 100644
--- a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
@@ -15,6 +15,9 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     path "software_versions_mqc.yml", emit: mqc_yml
     path "versions.yml"             , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def args = task.ext.args ?: ''
     template 'dumpsoftwareversions.py'
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
index d250eca0..ed6b8c50 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -15,6 +15,9 @@ process FASTQC {
     tuple val(meta), path("*.zip") , emit: zip
     path  "versions.yml"           , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def args = task.ext.args ?: ''
     // Add soft-links to original FastQs for consistent naming in pipeline
diff --git a/modules/nf-core/modules/kallisto/index/main.nf b/modules/nf-core/modules/kallisto/index/main.nf
index 4dc9c6d0..0f10e564 100644
--- a/modules/nf-core/modules/kallisto/index/main.nf
+++ b/modules/nf-core/modules/kallisto/index/main.nf
@@ -14,6 +14,9 @@ process KALLISTO_INDEX {
     path "kallisto" , emit: idx
     path "versions.yml" , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def args = task.ext.args ?: ''
     """
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index 3dceb162..0ff7cac1 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -15,6 +15,9 @@ process MULTIQC {
     path "*_plots"             , optional:true, emit: plots
     path "versions.yml"        , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def args = task.ext.args ?: ''
     """
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf
index 58c445d8..b83a4952 100644
--- a/modules/nf-core/modules/samtools/faidx/main.nf
+++ b/modules/nf-core/modules/samtools/faidx/main.nf
@@ -14,6 +14,9 @@ process SAMTOOLS_FAIDX {
     tuple val(meta), path ("*.fai"), emit: fai
     path "versions.yml"            , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def args = task.ext.args ?: ''
     """

From 9f3d658a59eb2f39ae714985fdad94de661492d5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Feb 2022 15:21:21 +0100
Subject: [PATCH 114/359] now supporting both data analysis and reference
 building

---
 main.nf | 12 +++++++++++-
 1 file changed, 11 insertions(+), 1 deletion(-)

diff --git a/main.nf b/main.nf
index bb980a4d..08cc839c 100644
--- a/main.nf
+++ b/main.nf
@@ -42,13 +42,23 @@ WorkflowMain.initialise(workflow, params, log)
 */
 
 include { BUILD_REFERENCES } from './workflows/build_references'
+include { RNAFUSION }        from './workflows/rnafusion'
+
 
 //
 // WORKFLOW: Run main nf-core/rnafusion analysis pipeline
 //
 workflow NFCORE_RNAFUSION {
 
-    BUILD_REFERENCES ()
+    if (params.build_references) {
+
+        BUILD_REFERENCES ()
+
+    } else {
+
+        RNAFUSION()
+
+    }
 
 }
 

From ad02db804c66e3a7e1f2b789b19ae10c667bf682 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Feb 2022 15:24:06 +0100
Subject: [PATCH 115/359] declare arguments for path to references

---
 nextflow.config      | 10 +++++++++
 nextflow_schema.json | 48 ++++++++++++++++++++++++++++++++++++++++----
 2 files changed, 54 insertions(+), 4 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index b88fff4a..583977d8 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -42,6 +42,16 @@ params {
     starindex                  = false
     starfusion                 = false
 
+    // Path to references
+    arriba_ref                    = "${params.genomes_base}/arriba"
+    ensembl_ref                   = "${params.genomes_base}/ensembl"
+    ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
+    fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
+    pizzly_ref                    = "${params.genomes_base}/pizzly"
+    squid_ref                     = "${params.genomes_base}/squid"
+    starfusion_ref                = "${params.genomes_base}/starfusion"
+    starindex_ref                 = "${params.genomes_base}/starindex
+
 
     // Boilerplate options
     outdir                     = './results'
diff --git a/nextflow_schema.json b/nextflow_schema.json
index bb3c2fe1..0a93a2b9 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -76,41 +76,81 @@
                     "description": "Build or run arriba references/analyses",
                     "default": "false"
                 },
+                "arriba_ref": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to arriba references"
+                },
+                "ensembl_ref": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to ensembl references"
+                },
                 "ericscript": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
                     "description": "Build or run ericscript references/analyses",
                     "default": "false"
                 },
+                "ericscript_ref": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to ericscript references"
+                },
                 "fusioncatcher": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
                     "description": "Build or run fusioncatcher references/analyses",
                     "default": "false"
                 },
+                "fusioncatcher_ref": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to fusioncatcher references"
+                },
                 "pizzly": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
                     "description": "Build or run pizzly references/analyses",
                     "default": "false"
                 },
+                "pizzly_ref": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to pizzly references"
+                },
                 "squid": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
                     "description": "Build or run squid references/analyses",
                     "default": "false"
                 },
-                "starindex": {
-                    "type": "boolean",
+                "squid_ref": {
+                    "type": "string",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run starindex references/analyses",
-                    "default": "false"
+                    "description": "Path to squid references"
                 },
                 "starfusion": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
                     "description": "Build or run starfusion references/analyses",
                     "default": "false"
+                },
+                "starfusion_ref": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to starfusion references"
+                },
+                "starindex": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run starindex references/analyses",
+                    "default": "false"
+                },
+                "starindex_ref": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to starindex references"
                 }
             }
         },

From 6debd09f0d36f9632cc471376b0fd21b198a8980 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Feb 2022 15:26:03 +0100
Subject: [PATCH 116/359] add star genomegenerate from nf-core modules

---
 .../modules/star/genomegenerate/main.nf       | 69 +++++++++++++++++++
 .../modules/star/genomegenerate/meta.yml      | 37 ++++++++++
 2 files changed, 106 insertions(+)
 create mode 100644 modules/nf-core/modules/star/genomegenerate/main.nf
 create mode 100644 modules/nf-core/modules/star/genomegenerate/meta.yml

diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf
new file mode 100644
index 00000000..50d280dd
--- /dev/null
+++ b/modules/nf-core/modules/star/genomegenerate/main.nf
@@ -0,0 +1,69 @@
+process STAR_GENOMEGENERATE {
+    tag "$fasta"
+    label 'process_high'
+
+    // Note: 2.7X indices incompatible with AWS iGenomes.
+    conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.13 conda-forge::gawk=5.1.0" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0' :
+        'quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0' }"
+
+    input:
+    path fasta
+    path gtf
+
+    output:
+    path "star"         , emit: index
+    path "versions.yml" , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args_list = args.tokenize()
+    def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
+    if (args_list.contains('--genomeSAindexNbases')) {
+        """
+        mkdir star
+        STAR \\
+            --runMode genomeGenerate \\
+            --genomeDir star/ \\
+            --genomeFastaFiles $fasta \\
+            --sjdbGTFfile $gtf \\
+            --runThreadN $task.cpus \\
+            $memory \\
+            $args
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            star: \$(STAR --version | sed -e "s/STAR_//g")
+            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+            gawk: \$(echo \$(gawk --version 2>&1) | sed 's/^.*GNU Awk //; s/, .*\$//')
+        END_VERSIONS
+        """
+    } else {
+        """
+        samtools faidx $fasta
+        NUM_BASES=`gawk '{sum = sum + \$2}END{if ((log(sum)/log(2))/2 - 1 > 14) {printf "%.0f", 14} else {printf "%.0f", (log(sum)/log(2))/2 - 1}}' ${fasta}.fai`
+
+        mkdir star
+        STAR \\
+            --runMode genomeGenerate \\
+            --genomeDir star/ \\
+            --genomeFastaFiles $fasta \\
+            --sjdbGTFfile $gtf \\
+            --runThreadN $task.cpus \\
+            --genomeSAindexNbases \$NUM_BASES \\
+            $memory \\
+            $args
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            star: \$(STAR --version | sed -e "s/STAR_//g")
+            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+            gawk: \$(echo \$(gawk --version 2>&1) | sed 's/^.*GNU Awk //; s/, .*\$//')
+        END_VERSIONS
+        """
+    }
+}
diff --git a/modules/nf-core/modules/star/genomegenerate/meta.yml b/modules/nf-core/modules/star/genomegenerate/meta.yml
new file mode 100644
index 00000000..04ade195
--- /dev/null
+++ b/modules/nf-core/modules/star/genomegenerate/meta.yml
@@ -0,0 +1,37 @@
+name: star_genomegenerate
+description: Create index for STAR
+keywords:
+  - index
+  - fasta
+  - genome
+  - reference
+tools:
+  - star:
+      description: |
+        STAR is a software package for mapping DNA sequences against
+        a large reference genome, such as the human genome.
+      homepage: https://github.com/alexdobin/STAR
+      manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
+      doi: 10.1093/bioinformatics/bts635
+      licence: ['MIT']
+input:
+  - fasta:
+      type: file
+      description: Fasta file of the reference genome
+  - gtf:
+      type: file
+      description: GTF file of the reference genome
+
+output:
+  - index:
+      type: directory
+      description: Folder containing the star index files
+      pattern: "star"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@kevinmenden"
+  - "@drpatelh"

From 796f7af78e383abe694380cddc59e089444237b5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Feb 2022 15:52:18 +0100
Subject: [PATCH 117/359] ensembl generates gtf and chr.gtf, starfusion refs
 built with chrgtf and fix to starfusion download binPath

---
 modules/local/ensembl/main.nf             | 4 ++++
 modules/local/starfusion/download/main.nf | 3 +--
 workflows/build_references.nf             | 2 +-
 3 files changed, 6 insertions(+), 3 deletions(-)

diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 2521a97b..0d2eec43 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -16,6 +16,7 @@ process ENSEMBL_DOWNLOAD {
     path "versions.yml"                                                   , emit: versions
     path "Homo_sapiens.${params.genome}.${ensembl_version}.all.fa"        , emit: fasta
     path "Homo_sapiens.${params.genome}.${ensembl_version}.gtf"           , emit: gtf
+    path "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"       , emit: chrgtf
     path "Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz", emit: transcript
 
     script:
@@ -24,10 +25,12 @@ process ENSEMBL_DOWNLOAD {
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
 
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
+    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz
 
     gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa
     gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
+    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
 
     echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """
@@ -36,6 +39,7 @@ process ENSEMBL_DOWNLOAD {
     """
     touch "Homo_sapiens.${params.genome}.${ensembl_version}.all.fa"
     touch "Homo_sapiens.${params.genome}.${ensembl_version}.gtf"
+    touch "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"
     touch "Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz"
 
     touch versions.yml
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index c6bda87f..16ba4ed0 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -1,6 +1,5 @@
 process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
-    label 'process_high'
 
     conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
@@ -17,7 +16,7 @@ process STARFUSION_DOWNLOAD {
     path "*"  , emit: reference
 
     script:
-    def binPath = { workflow.containerEngine == "singularity" || workflow.containerEngine == "docker" ? "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl" : "prep_genome_lib.pl" }
+    def binPath = ( params.enable_conda ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl" )
     """
     export TMPDIR=/tmp
 
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 5444dbc7..bd0b9b45 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -43,7 +43,7 @@ workflow BUILD_REFERENCES {
     }
 
     if (params.starfusion || params.all) {
-        STARFUSION_DOWNLOAD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
+        STARFUSION_DOWNLOAD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
     }
 
 }

From 4af8ce7f7698f80869a3011101172ef77a21f8a4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 7 Feb 2022 11:34:47 +0100
Subject: [PATCH 118/359] add star/align nf-core module

---
 modules/nf-core/modules/star/align/main.nf  | 71 ++++++++++++++++++
 modules/nf-core/modules/star/align/meta.yml | 81 +++++++++++++++++++++
 2 files changed, 152 insertions(+)
 create mode 100644 modules/nf-core/modules/star/align/main.nf
 create mode 100644 modules/nf-core/modules/star/align/meta.yml

diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf
new file mode 100644
index 00000000..7c965d07
--- /dev/null
+++ b/modules/nf-core/modules/star/align/main.nf
@@ -0,0 +1,71 @@
+process STAR_ALIGN {
+    tag "$meta.id"
+    label 'process_high'
+
+    // Note: 2.7X indices incompatible with AWS iGenomes.
+    conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0' :
+        'quay.io/biocontainers/star:2.7.9a--h9ee0642_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    path  index
+    path  gtf
+    val star_ignore_sjdbgtf
+    val seq_platform
+    val seq_center
+
+    output:
+    tuple val(meta), path('*d.out.bam')       , emit: bam
+    tuple val(meta), path('*Log.final.out')   , emit: log_final
+    tuple val(meta), path('*Log.out')         , emit: log_out
+    tuple val(meta), path('*Log.progress.out'), emit: log_progress
+    path  "versions.yml"                      , emit: versions
+
+    tuple val(meta), path('*sortedByCoord.out.bam')  , optional:true, emit: bam_sorted
+    tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript
+    tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted
+    tuple val(meta), path('*fastq.gz')               , optional:true, emit: fastq
+    tuple val(meta), path('*.tab')                   , optional:true, emit: tab
+    tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def ignore_gtf      = star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
+    def seq_platform    = seq_platform ? "'PL:$seq_platform'" : ""
+    def seq_center      = seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform "
+    def out_sam_type    = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
+    def mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
+    """
+    STAR \\
+        --genomeDir $index \\
+        --readFilesIn $reads  \\
+        --runThreadN $task.cpus \\
+        --outFileNamePrefix $prefix. \\
+        $out_sam_type \\
+        $ignore_gtf \\
+        $seq_center \\
+        $args
+
+    $mv_unsorted_bam
+
+    if [ -f ${prefix}.Unmapped.out.mate1 ]; then
+        mv ${prefix}.Unmapped.out.mate1 ${prefix}.unmapped_1.fastq
+        gzip ${prefix}.unmapped_1.fastq
+    fi
+    if [ -f ${prefix}.Unmapped.out.mate2 ]; then
+        mv ${prefix}.Unmapped.out.mate2 ${prefix}.unmapped_2.fastq
+        gzip ${prefix}.unmapped_2.fastq
+    fi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        star: \$(STAR --version | sed -e "s/STAR_//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/star/align/meta.yml b/modules/nf-core/modules/star/align/meta.yml
new file mode 100644
index 00000000..2d78b81a
--- /dev/null
+++ b/modules/nf-core/modules/star/align/meta.yml
@@ -0,0 +1,81 @@
+name: star_align
+description: Align reads to a reference genome using STAR
+keywords:
+  - align
+  - fasta
+  - genome
+  - reference
+tools:
+  - star:
+      description: |
+        STAR is a software package for mapping DNA sequences against
+        a large reference genome, such as the human genome.
+      homepage: https://github.com/alexdobin/STAR
+      manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
+      doi: 10.1093/bioinformatics/bts635
+      licence: ['MIT']
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
+  - index:
+      type: directory
+      description: STAR genome index
+      pattern: "star"
+output:
+  - bam:
+      type: file
+      description: Output BAM file containing read alignments
+      pattern: "*.{bam}"
+  - log_final:
+      type: file
+      description: STAR final log file
+      pattern: "*Log.final.out"
+  - log_out:
+      type: file
+      description: STAR lot out file
+      pattern: "*Log.out"
+  - log_progress:
+      type: file
+      description: STAR log progress file
+      pattern: "*Log.progress.out"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - bam_sorted:
+      type: file
+      description: Sorted BAM file of read alignments (optional)
+      pattern: "*sortedByCoord.out.bam"
+  - bam_transcript:
+      type: file
+      description: Output BAM file of transcriptome alignment (optional)
+      pattern: "*toTranscriptome.out.bam"
+  - bam_unsorted:
+      type: file
+      description: Unsorted BAM file of read alignments (optional)
+      pattern: "*Aligned.unsort.out.bam"
+  - fastq:
+      type: file
+      description: Unmapped FastQ files (optional)
+      pattern: "*fastq.gz"
+  - tab:
+      type: file
+      description: STAR output tab file(s) (optional)
+      pattern: "*.tab"
+  - junction:
+      type: file
+      description: STAR chimeric junction output file (optional)
+      pattern: "*.out.junction"
+
+authors:
+  - "@kevinmenden"
+  - "@drpatelh"
+  - "@praveenraj2018"

From e23f8ab2a861d214fd1b5482778579303df37d00 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 7 Feb 2022 15:55:46 +0100
Subject: [PATCH 119/359] add nfcore module arriba

---
 modules/nf-core/modules/arriba/main.nf  | 42 +++++++++++++++++++
 modules/nf-core/modules/arriba/meta.yml | 54 +++++++++++++++++++++++++
 2 files changed, 96 insertions(+)
 create mode 100644 modules/nf-core/modules/arriba/main.nf
 create mode 100644 modules/nf-core/modules/arriba/meta.yml

diff --git a/modules/nf-core/modules/arriba/main.nf b/modules/nf-core/modules/arriba/main.nf
new file mode 100644
index 00000000..e0d30b45
--- /dev/null
+++ b/modules/nf-core/modules/arriba/main.nf
@@ -0,0 +1,42 @@
+process ARRIBA {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::arriba=2.1.0" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/arriba:2.1.0--h3198e80_1' :
+        'quay.io/biocontainers/arriba:2.1.0--h3198e80_1' }"
+
+    input:
+    tuple val(meta), path(bam)
+    path fasta
+    path gtf
+
+    output:
+    tuple val(meta), path("*.fusions.tsv")          , emit: fusions
+    tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail
+    path "versions.yml"                             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def blacklist = (args.contains('-b')) ? '' : '-f blacklist'
+    """
+    arriba \\
+        -x $bam \\
+        -a $fasta \\
+        -g $gtf \\
+        -o ${prefix}.fusions.tsv \\
+        -O ${prefix}.fusions.discarded.tsv \\
+        $blacklist \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        arriba: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/arriba/meta.yml b/modules/nf-core/modules/arriba/meta.yml
new file mode 100644
index 00000000..6ca16dab
--- /dev/null
+++ b/modules/nf-core/modules/arriba/meta.yml
@@ -0,0 +1,54 @@
+name: arriba
+description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
+keywords:
+  - fusion
+  - arriba
+tools:
+  - arriba:
+      description: Fast and accurate gene fusion detection from RNA-Seq data
+      homepage: https://github.com/suhrig/arriba
+      documentation: https://arriba.readthedocs.io/en/latest/
+      tool_dev_url: https://github.com/suhrig/arriba
+      doi: "10.1101/gr.257246.119"
+      licence: ['MIT']
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - fasta:
+      type: file
+      description: Assembly FASTA file
+      pattern: "*.{fasta}"
+  - gtf:
+      type: file
+      description: Annotation GTF file
+      pattern: "*.{gtf}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - fusions:
+      type: file
+      description: File contains fusions which pass all of Arriba's filters.
+      pattern: "*.{fusions.tsv}"
+  - fusions_fail:
+      type: file
+      description: File contains fusions that Arriba classified as an artifact or that are also observed in healthy tissue.
+      pattern: "*.{fusions.discarded.tsv}"
+
+authors:
+  - "@praveenraj2018"

From 3f51a9675ffd7d6332d45ea3355b24779e4941a7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:09:08 +0100
Subject: [PATCH 120/359] use arriba and star/align from nfcore, add publish
 dirs and args for arriba, star/genomegenerate and ericscript

---
 conf/genomes.config |  4 +--
 conf/modules.config | 84 +++++++++++++++++++++++++++++++++++++++++----
 main.nf             |  2 ++
 modules.json        |  6 ++++
 4 files changed, 87 insertions(+), 9 deletions(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index 251a4a21..5bdd6d58 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -21,7 +21,7 @@ params {
             pizzly_ref                    = "${params.genomes_base}/pizzly"
             squid_ref                     = "${params.genomes_base}/squid"
             starfusion_ref                = "${params.genomes_base}/starfusion"
-            starindex_ref                 = "${params.genomes_base}/starindex
+            starindex_ref                 = "${params.genomes_base}/star"
 
         }
         'GRCh37' {
@@ -35,7 +35,7 @@ params {
             pizzly_ref                    = "${params.genomes_base}/pizzly"
             squid_ref                     = "${params.genomes_base}/squid"
             starfusion_ref                = "${params.genomes_base}/starfusion"
-            starindex_ref                 = "${params.genomes_base}/starindex
+            starindex_ref                 = "${params.genomes_base}/star"
 
         }
     }
diff --git a/conf/modules.config b/conf/modules.config
index 32ce380d..875a3c00 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -47,6 +47,40 @@ process {
         ]
     }
 
+    withName: ARRIBA_DOWNLOAD {
+        publishDir = [
+            path: { "${params.genomes_base}/arriba" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
+    withName: ARRIBA {
+        publishDir = [
+            path: { "${params.outdir}/arriba" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+        ext.args = '-b ${params.arriba_ref}/blacklist_hg38_GRCh38_2018-11-04.tsv \
+        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_2019-07-05.gff3'
+    }
+
+    withName: ERICSCRIPT_DOWNLOAD {
+    publishDir = [
+        path: { "${params.genomes_base}/ericscript" },
+        mode: 'copy',
+        saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+    ]
+}
+    withName: STAR_GENOMEGENERATE {
+        ext.args = "--sjdbOverhang ${params.read_length - 1}"
+        publishDir = [
+            path: { "${params.genomes_base}" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+    ]
+    }
+
     withName: FUSIONCATCHER_DOWNLOAD {
         ext.args = "--continue --tries=42"
     }
@@ -66,15 +100,51 @@ process {
         ]
     }
 
-    withName: ERICSCRIPT_DOWNLOAD {
-    publishDir = [
-        path: { "${params.genomes_base}/ericscript" },
-        mode: 'copy',
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
-    ]
-}
+//TODO document changes from rnafusion 1.0 to use star-fusion recommendations
+    withName: STAR_FOR_STARFUSION {
+        ext.args = '--twopassMode Basic \
+        --outReadsUnmapped None \
+        --readFilesCommand zcat \
+        --outSAMstrandField intronMotif \
+        --outSAMunmapped Within \
+        --chimSegmentMin 12 \
+        --chimJunctionOverhangMin 8 \
+        --chimOutJunctionFormat 1 \
+        --alignSJDBoverhangMin 10 \
+        --alignMatesGapMax 100000 \
+        --alignIntronMax 100000 \
+        --alignSJstitchMismatchNmax 5 -1 5 5 \
+        --chimMultimapScoreRange 3 \
+        --chimScoreJunctionNonGTAG -4 \
+        --chimMultimapNmax 20 \
+        --chimNonchimScoreDropMin 10 \
+        --peOverlapNbasesMin 12 \
+        --peOverlapMMp 0.1 \
+        --alignInsertionFlush Right \
+        --alignSplicedMateMapLminOverLmate 0 \
+        --alignSplicedMateMapLmin 30 \
+        --chimOutType Junctions'
+    }
 
 
+    withName: STAR_FOR_ARRIBA {
+        ext.args = '--readFilesCommand zcat \
+        --outSAMtype BAM Unsorted \
+        --outSAMunmapped Within \
+        --outBAMcompression 0 \
+        --outFilterMultimapNmax 50 \
+        --peOverlapNbasesMin 10 \
+        --alignSplicedMateMapLminOverLmate 0.5 \
+        --alignSJstitchMismatchNmax 5 -1 5 5 \
+        --chimSegmentMin 10 \
+        --chimOutType WithinBAM HardClip \
+        --chimJunctionOverhangMin 10 \
+        --chimScoreDropMax 30 \
+        --chimScoreJunctionNonGTAG 0 \
+        --chimScoreSeparation 1 \
+        --chimSegmentReadGapMax 3 \
+        --chimMultimapNmax 50'
+    }
 /*
     withName: PIZZLY {
         ext.args = = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
diff --git a/main.nf b/main.nf
index 08cc839c..1d708fd0 100644
--- a/main.nf
+++ b/main.nf
@@ -18,6 +18,8 @@ nextflow.enable.dsl = 2
 */
 
 params.fasta             = WorkflowMain.getGenomeAttribute(params, 'fasta')
+params.genomes_base      = WorkflowMain.getGenomeAttribute(params, 'genomes_base')
+
 
 /*
 ========================================================================================
diff --git a/modules.json b/modules.json
index 166e7244..8c0ca267 100644
--- a/modules.json
+++ b/modules.json
@@ -3,6 +3,9 @@
     "homePage": "https://github.com/nf-core/rnafusion",
     "repos": {
         "nf-core/modules": {
+            "arriba": {
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+            },
             "custom/dumpsoftwareversions": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
@@ -18,6 +21,9 @@
             "samtools/faidx": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
+            "star/align": {
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+            },
             "star/genomegenerate": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             }

From 915285c605a769a899787b731a0d1c48cf28bfa8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:10:55 +0100
Subject: [PATCH 121/359] cleanup ericscript

---
 modules/local/ericscript/detect/main.nf | 21 +++++++++++++++------
 1 file changed, 15 insertions(+), 6 deletions(-)

diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
index dc77ff42..157988ce 100644
--- a/modules/local/ericscript/detect/main.nf
+++ b/modules/local/ericscript/detect/main.nf
@@ -14,22 +14,31 @@ process ERICSCRIPT {
     path reference
 
     output:
-    path "versions.yml"                                                    , emit: versions
-    tuple val(meta), path "./tmp/${prefix}.ericscript.results.filtered.tsv", emit: fusions
-    tuple val(meta), path "./tmp/${prefix}.ericscript.results.total.tsv"   , emit: fusions_total
+    tuple val(meta), path("*.results.filtered.tsv"), emit: fusions
+    tuple val(meta), path("*.results.total.tsv")   , emit: fusions_total
+    path "versions.yml"                           , emit: versions
 
     script:
     def args = task.ext.args ?: ''
-    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def prefix = task.ext.prefix ?: "${meta.id}"
     """
+
     ericscript.pl \\
         -db $reference \\
-        -name ${prefix}.ericscript \\
+        -name ${prefix} \\
         -p $task.cpus \\
-        -o ./tmp \\
+        -o . \\
         $reads \\
         $args
 
     echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
+
+    """
+
+    stub:
+    """
+    touch ${prefix}.results.filtered.tsv
+    touch ${prefix}.results.total.tsv
+    touch versions.yml
     """
 }

From 7c77408cb6dc2c39f9d0ac17fca2e220a958c801 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:11:43 +0100
Subject: [PATCH 122/359] modify ericscript download publish dir

---
 modules/local/ericscript/download/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/ericscript/download/main.nf b/modules/local/ericscript/download/main.nf
index c72bc69e..95510c15 100644
--- a/modules/local/ericscript/download/main.nf
+++ b/modules/local/ericscript/download/main.nf
@@ -11,7 +11,7 @@ process ERICSCRIPT_DOWNLOAD {
 
     output:
     path "versions.yml"  , emit: versions
-    path "homo_sapiens/*", emit: reference
+    path "*"             , emit: reference
 
     script:
     """

From 5ed3eac979efb14edbdea196cc757c1dc9491270 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:12:20 +0100
Subject: [PATCH 123/359] fusioncatcher download: add symlink to current

---
 modules/local/fusioncatcher/download/main.nf | 1 +
 1 file changed, 1 insertion(+)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 65a3db52..76a7b8ef 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -33,6 +33,7 @@ process FUSIONCATCHER_DOWNLOAD {
             -o human_${human_version} \\
             $args2
     fi
+    ln -s human_v102 current
 
     fusioncatcher --version | sed 's/fusioncatcher.py //' > versions.yml
     """

From 2423af552cacaa990e4dd40e89b41f0bc6b5ed55 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:12:59 +0100
Subject: [PATCH 124/359] fusioncatcher meta version.txt to versions.yml

---
 modules/local/fusioncatcher/download/meta.yml | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/fusioncatcher/download/meta.yml b/modules/local/fusioncatcher/download/meta.yml
index 1e1e7426..128e8f88 100644
--- a/modules/local/fusioncatcher/download/meta.yml
+++ b/modules/local/fusioncatcher/download/meta.yml
@@ -18,10 +18,10 @@ input:
       pattern: "*"
 
 output:
-  - version:
-      type: file
-      description: File containing software version
-      pattern: "*.{version.txt}"
+  - versions:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
   - reference:
       type: directory
       description: directory containing the genome resource files required for fusioncatcher

From b348cc911c85641924ad08c95a303079ff27e9d7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:13:40 +0100
Subject: [PATCH 125/359] preliminary fusioncatcher detect work

---
 modules/local/fusioncatcher/detect/main.nf | 28 ++++++++++++++--------
 1 file changed, 18 insertions(+), 10 deletions(-)

diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 15a552ef..8921c7f6 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -4,13 +4,15 @@ process FUSIONCATCHER {
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_1"
+        // container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.30--hdfd78af_1"
+        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_2"
     } else {
-        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_1"
+        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.30--0"
+        // container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_1"
     }
 
     input:
-    tuple val(meta), path(reads)
+    tuple val(meta), path(fasta)
     path reference
 
     output:
@@ -21,19 +23,25 @@ process FUSIONCATCHER {
 
     script:
     def args = task.ext.args ?: ''
-    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def reads = fasta.toString().replace(" ", ",")
     """
     fusioncatcher.py \\
         -d $reference \\
         -i $reads \\
         -p $task.cpus \\
         -o . \\
+        --skip-blat \\
         $args
-
-    mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher.fusion-genes.txt
-    mv summary_candidate_fusions.txt ${prefix}.fusioncatcher.summary.txt
-    mv fusioncatcher.log ${prefix}.fusioncatcher.log
-
-    fusioncatcher --version | sed 's/fusioncatcher.py //' > versions.yml
+    touch versions.yml
     """
 }
+
+
+    // mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher.fusion-genes.txt
+    // mv summary_candidate_fusions.txt ${prefix}.fusioncatcher.summary.txt
+    // mv fusioncatcher.log ${prefix}.fusioncatcher.log
+    //  fusioncatcher --version | sed 's/fusioncatcher.py //' > versions.yml
+    // touch ${prefix}.fusioncatcher.fusion-genes.txt
+    // touch ${prefix}.fusioncatcher.summary.txt
+    // touch ${prefix}.fusioncatcher.log

From 4bdc23219c314019e9ccd72def606bf60c179127 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:15:15 +0100
Subject: [PATCH 126/359] arriba detect cleanup

---
 modules/local/arriba/detect/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/arriba/detect/main.nf b/modules/local/arriba/detect/main.nf
index a543c82f..fb6a3851 100644
--- a/modules/local/arriba/detect/main.nf
+++ b/modules/local/arriba/detect/main.nf
@@ -21,7 +21,7 @@ process ARRIBA {
     path "versions.yml"                             , emit: versions
 
     script:
-    def prefix    = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def prefix = task.ext.prefix ?: "${meta.id}"
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
     """

From 922c94296b8be55e5d330e8cee1d8e76817791cf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:15:41 +0100
Subject: [PATCH 127/359] rename starindex_ref to star

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 583977d8..552e4d8b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -50,7 +50,7 @@ params {
     pizzly_ref                    = "${params.genomes_base}/pizzly"
     squid_ref                     = "${params.genomes_base}/squid"
     starfusion_ref                = "${params.genomes_base}/starfusion"
-    starindex_ref                 = "${params.genomes_base}/starindex
+    starindex_ref                 = "${params.genomes_base}/star"
 
 
     // Boilerplate options

From 7fd9d134d147338c5331c385d66a511c4fde5e1b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:16:36 +0100
Subject: [PATCH 128/359] star/genomegenerate and kallisto to nfcore modules
 subsection in buildreference.nf

---
 workflows/build_references.nf | 11 +++++++++--
 1 file changed, 9 insertions(+), 2 deletions(-)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index bd0b9b45..fbbc75c9 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -6,12 +6,19 @@
 
 include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'
 include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'
-include { STAR_GENOMEGENERATE }             from '../modules/local/star/genomegenerate/main'
 include { ERICSCRIPT_DOWNLOAD }             from '../modules/local/ericscript/download/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
-include { KALLISTO_INDEX as PIZZLY_INDEX }  from '../modules/nf-core/modules/kallisto/index/main'
 include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
 
+/*
+========================================================================================
+    IMPORT NF-CORE MODULES/SUBWORKFLOWS
+========================================================================================
+*/
+
+include { STAR_GENOMEGENERATE }             from '../modules/nf-core/modules/star/genomegenerate/main'
+include { KALLISTO_INDEX as PIZZLY_INDEX }  from '../modules/nf-core/modules/kallisto/index/main'
+
 /*
 ========================================================================================
     RUN MAIN WORKFLOW

From 17bc6521b53795e20a8c62ae918bd032577d6f12 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:17:59 +0100
Subject: [PATCH 129/359] preliminary rnafusion workflow for data analysis with
 multiqc and fastqc commented out

---
 workflows/rnafusion.nf | 137 ++++++++++++++++++++++++++---------------
 1 file changed, 89 insertions(+), 48 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index c6a8d53f..a0415d58 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -11,11 +11,22 @@ WorkflowRnafusion.initialise(params, log)
 
 // TODO nf-core: Add all file path parameters for the pipeline to the list below
 // Check input path parameters to see if they exist
-def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ]
+
+
+//TODO problem here as all the folders have to exist
+def checkPathParamList = [
+    params.input, params.multiqc_config, params.fasta,
+    params.genomes_base,
+    params.fusioncatcher_ref, params.starfusion_ref,
+    params.arriba_ref, params.ericscript_ref
+]
+
 for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
 
 // Check mandatory parameters
 if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' }
+// if (!params.build_references && !params.genomes_base) { exit 1 }
+
 
 /*
 ========================================================================================
@@ -35,7 +46,16 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 //
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
-include { INPUT_CHECK } from '../subworkflows/local/input_check'
+include { INPUT_CHECK                   } from '../subworkflows/local/input_check'
+// include { ARRIBA                        } from '../modules/local/arriba/detect/main'
+// include { PREPARE_GENOME                } from '../subworkflows/local/prepare_genome'
+
+// include { STARFUSION                 }   from '../modules/local/starfusion/detection/main'
+include { FUSIONCATCHER                 }   from '../modules/local/fusioncatcher/detect/main'
+include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
+
+include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
+include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
 
 /*
 ========================================================================================
@@ -50,11 +70,8 @@ include { FASTQC                      } from '../modules/nf-core/modules/fastqc/
 include { MULTIQC                     } from '../modules/nf-core/modules/multiqc/main'
 include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main'
 
-include { FASTQC                      }   from '../modules/nf-core/modules/fastqc/main'           addParams( options: modules['fastqc'] )
-include { MULTIQC                     }   from '../modules/nf-core/modules/multiqc/main'          addParams( options: multiqc_options   )
-include { FUSION_STAR_ARRIBA          }   from '../subworkflows/nf-core/arriba'                   addParams( star_align_options: modules['star_align'], arriba_options: modules['arriba_fusion'])
-include { STARFUSION                  }   from '../modules/local/starfusion/detection/main'       addParams( options: modules['starfusion'] )
-include { FUSIONCATCHER               }   from '../modules/local/fusioncatcher/detection/main'    addParams( options: modules['fusioncatcher'] )
+
+
 
 /*
 ========================================================================================
@@ -80,66 +97,90 @@ workflow RNAFUSION {
     //
     // MODULE: Run FastQC
     //
-    FASTQC (
-        INPUT_CHECK.out.reads
-    )
-    ch_versions = ch_versions.mix(FASTQC.out.versions.first())
+    // FASTQC (
+    //     INPUT_CHECK.out.reads
+    // )
+    // ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
-    CUSTOM_DUMPSOFTWAREVERSIONS (
-        ch_versions.unique().collectFile(name: 'collated_versions.yml')
-    )
+    // CUSTOM_DUMPSOFTWAREVERSIONS (
+    //     ch_versions.unique().collectFile(name: 'collated_versions.yml')
+    // )
 
     //
     // MODULE: MultiQC
     //
-    workflow_summary    = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params)
-    ch_workflow_summary = Channel.value(workflow_summary)
-
-    ch_multiqc_files = Channel.empty()
-    ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
-    ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
-    ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
-    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
-
-    MULTIQC (
-        ch_multiqc_files.collect()
-    )
+    // workflow_summary    = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params)
+    // ch_workflow_summary = Channel.value(workflow_summary)
+
+    // ch_multiqc_files = Channel.empty()
+    // ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
+    // ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
+    // ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    // ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
+    // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+
+    // MULTIQC (
+    //     ch_multiqc_files.collect()
+    // )
+
+    // multiqc_report       = MULTIQC.out.report.toList()
+    // ch_versions          = ch_versions.mix(MULTIQC.out.versions)
+
+
+
+    if (params.ericscript){
+        // def checkPathParamList = []
+        // for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
+
+        ERICSCRIPT (
+            INPUT_CHECK.out.reads,
+            params.ericscript_ref
+        )
+    }
+
+
+
+
+
+
 
-    multiqc_report       = MULTIQC.out.report.toList()
-    ch_versions    = ch_versions.mix(MULTIQC.out.versions)
     // Run STAR alignment and Arriba
     if (params.arriba){
-        ch_genome_bam     = Channel.empty()
-        ch_arriba_fusions = Channel.empty()
-        FUSION_STAR_ARRIBA (
-            ch_reads,
-            PREPARE_GENOME.out.star_index,
-            PREPARE_GENOME.out.fasta,
-            PREPARE_GENOME.out.gtf
+        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+
+        ARRIBA_WORKFLOW (
+            INPUT_CHECK.out.reads,
+            params.starindex_ref,
+            gtf
         )
-        ch_genome_bam     = FUSION_STAR_ARRIBA.out.bam
-        ch_arriba_fusions = FUSION_STAR_ARRIBA.out.fusions
     }
 
+
+    // Run ericscript
+    // if (params.ericscript){
+    //     ERICSCRIPT (
+    //         INPUT_CHECK.out.reads,
+    //         params.ericscript_ref
+    //     )
+    // }
+
+
     //Run STAR fusion
     if (params.starfusion){
-        ch_star_fusions = Channel.empty()
-        STARFUSION (
-            ch_reads,
-            PREPARE_GENOME.out.starfusion_resource
+        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
+        STARFUSION_WORKFLOW (
+            INPUT_CHECK.out.reads,
+            params.starindex_ref,
+            gtf,
+            params.starfusion_ref
         )
-        ch_starfusion_fusions = STARFUSION.out.fusions
     }
-
     //Run FusionCatcher
     if (params.fusioncatcher){
-        ch_fusioncather_fusions = Channel.empty()
         FUSIONCATCHER (
-            ch_reads,
-            PREPARE_GENOME.out.fusioncatcher_resource
+            INPUT_CHECK.out.reads,
+            params.fusioncatcher_ref
         )
-        ch_fusioncatcher_fusions = FUSIONCATCHER.out.fusions
     }
 }
 

From f3ca72604268c4a1cf26b00ab074fb295d466cb3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:18:37 +0100
Subject: [PATCH 130/359] adding of a new arriba subworkflow using nfcore
 modules

---
 subworkflows/local/arriba_workflow.nf | 34 +++++++++++++++++++++++++++
 1 file changed, 34 insertions(+)
 create mode 100644 subworkflows/local/arriba_workflow.nf

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
new file mode 100644
index 00000000..432d5b6d
--- /dev/null
+++ b/subworkflows/local/arriba_workflow.nf
@@ -0,0 +1,34 @@
+//
+// Check input samplesheet and get read channels
+//
+
+include { STAR_ALIGN as STAR_FOR_ARRIBA }    from '../../modules/nf-core/modules/star/align/main'
+include { ARRIBA }                           from '../../modules/nf-core/modules/arriba/main'
+
+
+workflow ARRIBA_WORKFLOW {
+    take:
+        fasta
+        index
+        gtf
+
+    main:
+        ch_versions = Channel.empty()
+
+        star_ignore_sjdbgtf = false
+        seq_platform = false
+        seq_center = false
+
+        STAR_FOR_ARRIBA( fasta, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+        ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
+
+        ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
+        ch_versions = ch_versions.mix(ARRIBA.out.versions)
+
+
+    emit:
+        ARRIBA.out.fusions
+        ARRIBA.out.fusions_fail
+        versions = ch_versions.ifEmpty(null)
+    }
+

From 9aad2261ee4d022c74241d82ff0cd417b18e3394 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 11:18:55 +0100
Subject: [PATCH 131/359] add a starfusion workflow

---
 subworkflows/local/starfusion_workflow.nf | 39 +++++++++++++++++++++++
 1 file changed, 39 insertions(+)
 create mode 100644 subworkflows/local/starfusion_workflow.nf

diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
new file mode 100644
index 00000000..ed5eaac2
--- /dev/null
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -0,0 +1,39 @@
+//
+// Check input samplesheet and get read channels
+//
+
+include { STAR_ALIGN as STAR_FOR_STARFUSION }    from '../../modules/nf-core/modules/star/align/main'
+include { STARFUSION }                           from '../../modules/local/starfusion/detect/main'
+
+
+workflow STARFUSION_WORKFLOW {
+    take:
+        reads
+        index
+        gtf
+        starfusion_ref
+
+    main:
+        ch_versions = Channel.empty()
+
+        star_ignore_sjdbgtf = false
+        seq_platform = false
+        seq_center = false
+
+        STAR_FOR_STARFUSION( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+        // ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
+        reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
+
+        STARFUSION( reads_junction, starfusion_ref)
+        // ch_versions = ch_versions.mix(STARFUSION.out.versions, starfusion_ref )
+
+    emit:
+        versions        = ch_versions.ifEmpty(null) // channel: [ versions.yml ]
+
+    // versions = ch_versions.ifEmpty(null)
+    // STARFUSION.out.fusions
+    // STARFUSION.out.abridged
+    // STARFUSION.out.coding_effect
+
+}
+

From 480c70946f0e9c7fe301427e76f057a7143536ab Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 15:54:38 +0100
Subject: [PATCH 132/359] arriba + starfusion to running status

---
 conf/modules.config                         | 28 +++++-
 modules/local/arriba/detect/main.nf         | 46 ----------
 modules/local/ericscript/detect/main.nf     |  2 +-
 modules/local/fusioncatcher/detect/meta.yml | 25 ++++--
 modules/local/star/align/main.nf            | 64 --------------
 modules/local/star/align/meta.yml           | 80 -----------------
 modules/local/star/genomegenerate/main.nf   | 37 --------
 modules/local/star/genomegenerate/meta.yml  | 36 --------
 modules/local/starfusion/detect/main.nf     | 33 ++++---
 modules/local/starfusion/detect/meta.yml    | 17 ++--
 subworkflows/local/arriba_workflow.nf       |  5 +-
 subworkflows/local/prepare_genome.nf        | 91 -------------------
 workflows/rnafusion.nf                      | 97 ++++++++++-----------
 13 files changed, 122 insertions(+), 439 deletions(-)
 delete mode 100644 modules/local/arriba/detect/main.nf
 delete mode 100644 modules/local/star/align/main.nf
 delete mode 100644 modules/local/star/align/meta.yml
 delete mode 100644 modules/local/star/genomegenerate/main.nf
 delete mode 100644 modules/local/star/genomegenerate/meta.yml
 delete mode 100644 subworkflows/local/prepare_genome.nf

diff --git a/conf/modules.config b/conf/modules.config
index 875a3c00..86ab19f1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -61,8 +61,8 @@ process {
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
-        ext.args = '-b ${params.arriba_ref}/blacklist_hg38_GRCh38_2018-11-04.tsv \
-        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_2019-07-05.gff3'
+        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_2018-11-04.tsv \
+        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_2019-07-05.gff3"
     }
 
     withName: ERICSCRIPT_DOWNLOAD {
@@ -87,6 +87,11 @@ process {
 
     withName: KALLISTO_INDEX {
         ext.args = '-k 31'
+        publishDir = [
+            path: { "${params.genomes_base}/pizzly" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
     }
 
     withName: STARFUSION_DOWNLOAD {
@@ -100,7 +105,7 @@ process {
         ]
     }
 
-//TODO document changes from rnafusion 1.0 to use star-fusion recommendations
+//TODO document changes from previous rnafusion version to use star-fusion recommendations
     withName: STAR_FOR_STARFUSION {
         ext.args = '--twopassMode Basic \
         --outReadsUnmapped None \
@@ -126,7 +131,7 @@ process {
         --chimOutType Junctions'
     }
 
-
+//TODO document changes from previous rnafusion version
     withName: STAR_FOR_ARRIBA {
         ext.args = '--readFilesCommand zcat \
         --outSAMtype BAM Unsorted \
@@ -145,6 +150,21 @@ process {
         --chimSegmentReadGapMax 3 \
         --chimMultimapNmax 50'
     }
+
+
+    //     withName: STAR_FOR_SQUID {
+    //     ext.args = '--twopassMode Basic \
+    //     --chimOutType SeparateSAMold \
+    //     --chimSegmentMin 20 \
+    //     --chimJunctionOverhangMin 12 \
+    //     --alignSJDBoverhangMin 10 \
+    //     --outReadsUnmapped Fastx \
+    //     --outSAMstrandField intronMotif \
+    //     --outSAMtype BAM SortedByCoordinate \
+    //     --readFilesCommand zcat'
+    // }
+
+
 /*
     withName: PIZZLY {
         ext.args = = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
diff --git a/modules/local/arriba/detect/main.nf b/modules/local/arriba/detect/main.nf
deleted file mode 100644
index fb6a3851..00000000
--- a/modules/local/arriba/detect/main.nf
+++ /dev/null
@@ -1,46 +0,0 @@
-process ARRIBA {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda (params.enable_conda ? "bioconda::arriba=2.1.0" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/arriba:2.1.0--h3198e80_1"
-    } else {
-        container "quay.io/biocontainers/arriba:2.1.0--h3198e80_1"
-    }
-
-    input:
-    tuple val(meta), path(reads)
-    path fasta
-    path gtf
-    path index
-
-    output:
-    tuple val(meta), path("*.fusions.tsv")          , emit: fusions
-    tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail
-    path "versions.yml"                             , emit: versions
-
-    script:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    """
-    STAR \\
-        --genomeDir $index \\
-        --readFilesIn $reads \\
-        --runThreadN $task.cpus \\
-        $args |
-
-    tee Aligned.out.bam |
-
-    arriba \\
-        -x /dev/stdin \\
-        -a $fasta \\
-        -g $gtf \\
-        -o ${prefix}.arriba.fusions.tsv \\
-        -O ${prefix}.arriba.fusions.discarded.tsv \\
-        $args2
-
-    echo \$(arriba -h | grep 'Version:' 2>&1) |  sed 's/Version:\s//' > versions.yml
-    """
-}
diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
index 157988ce..57b685c7 100644
--- a/modules/local/ericscript/detect/main.nf
+++ b/modules/local/ericscript/detect/main.nf
@@ -4,7 +4,7 @@ process ERICSCRIPT {
 
     conda (params.enable_conda ? "bioconda::ericscript=0.5.5" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/ericscript:0.5.5--hdfd78af_5"
+        container "https://depot.galaxyproject.org/singularity/ericscript:0.5.5--pl5.22.0r3.4.1_1"
     } else {
         container "quay.io/biocontainers/ericscript:0.5.5--hdfd78af_5"
     }
diff --git a/modules/local/fusioncatcher/detect/meta.yml b/modules/local/fusioncatcher/detect/meta.yml
index 81efa6fd..4f80fa58 100644
--- a/modules/local/fusioncatcher/detect/meta.yml
+++ b/modules/local/fusioncatcher/detect/meta.yml
@@ -2,7 +2,6 @@ name: fusioncatcher
 description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
 keywords:
   - fusioncatcher
-  - fusion
 tools:
   - fusioncatcher:
       description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
@@ -22,6 +21,10 @@ input:
       type: file
       description: FASTQ file
       pattern: "*.{fastq}"
+  - reference:
+      type: directory
+      description: Path to fusioncatcher references
+      pattern: "fusioncatcher-genome"
 
 output:
   - meta:
@@ -29,18 +32,22 @@ output:
       description: |
         Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
-  - version:
-      type: file
-      description: File containing software version
-      pattern: "*.{version.txt}"
-  - fusions:
+  - fusiongenes:
       type: file
       description: Final list of candidate fusion genes
-      pattern: "*.{fusioncatcher.txt}"
-  - bam:
+      pattern: "*.fusioncatcher.fusion-genes.txt"
+  - summary:
       type: file
       description: Summary of fusion results
-      pattern: "*.{fusioncatcher_summary.txt}"
+      pattern: "*.fusioncatcher_summary.txt"
+  - log:
+      type: file
+      description: Log of fusion results
+      pattern: "*.fusioncatcher.log"
+  - versions:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
 
 authors:
   - "@praveenraj2018"
diff --git a/modules/local/star/align/main.nf b/modules/local/star/align/main.nf
deleted file mode 100644
index 49e7d8ae..00000000
--- a/modules/local/star/align/main.nf
+++ /dev/null
@@ -1,64 +0,0 @@
-process STAR_ALIGN {
-    tag "$meta.id"
-    label 'process_high'
-
-    // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda (params.enable_conda ? 'bioconda::star=2.7.8a' : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container 'https://depot.galaxyproject.org/singularity/star:2.7.8a--h9ee0642_1'
-    } else {
-        container 'quay.io/biocontainers/star:2.7.8a--h9ee0642_1'
-    }
-
-    input:
-    tuple val(meta), path(reads)
-    path  index
-    path  gtf
-
-    output:
-    tuple val(meta), path('*d.out.bam')       , emit: bam
-    tuple val(meta), path('*Log.final.out')   , emit: log_final
-    tuple val(meta), path('*Log.out')         , emit: log_out
-    tuple val(meta), path('*Log.progress.out'), emit: log_progress
-    path 'versions.yml'                       , emit: versions
-
-    tuple val(meta), path('*sortedByCoord.out.bam')  , optional:true, emit: bam_sorted
-    tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript
-    tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted
-    tuple val(meta), path('*fastq.gz')               , optional:true, emit: fastq
-    tuple val(meta), path('*.tab')                   , optional:true, emit: tab
-    tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix          = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def ignore_gtf      = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
-    def seq_platform    = params.seq_platform ? "'PL:$params.seq_platform'" : ""
-    def seq_center      = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform "
-    def out_sam_type    = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
-    def mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
-    """
-    STAR \\
-        --genomeDir $index \\
-        --readFilesIn $reads  \\
-        --runThreadN $task.cpus \\
-        --outFileNamePrefix $prefix. \\
-        $out_sam_type \\
-        $ignore_gtf \\
-        $seq_center \\
-        $args
-
-    $mv_unsorted_bam
-
-    if [ -f ${prefix}.Unmapped.out.mate1 ]; then
-        mv ${prefix}.Unmapped.out.mate1 ${prefix}.unmapped_1.fastq
-        gzip ${prefix}.unmapped_1.fastq
-    fi
-    if [ -f ${prefix}.Unmapped.out.mate2 ]; then
-        mv ${prefix}.Unmapped.out.mate2 ${prefix}.unmapped_2.fastq
-        gzip ${prefix}.unmapped_2.fastq
-    fi
-
-    STAR --version | sed -e "s/STAR_//g" > versions.yml
-    """
-}
diff --git a/modules/local/star/align/meta.yml b/modules/local/star/align/meta.yml
deleted file mode 100644
index a589d145..00000000
--- a/modules/local/star/align/meta.yml
+++ /dev/null
@@ -1,80 +0,0 @@
-name: star_align
-description: Align reads to a reference genome using STAR
-keywords:
-  - align
-  - fasta
-  - genome
-  - reference
-tools:
-  - star:
-      description: |
-        STAR is a software package for mapping DNA sequences against
-        a large reference genome, such as the human genome.
-      homepage: https://github.com/alexdobin/STAR
-      manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
-      doi: 10.1093/bioinformatics/bts635
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-        respectively.
-  - index:
-      type: directory
-      description: STAR genome index
-      pattern: "star"
-output:
-  - bam:
-      type: file
-      description: Output BAM file containing read alignments
-      pattern: "*.{bam}"
-  - log_final:
-      type: file
-      description: STAR final log file
-      pattern: "*Log.final.out"
-  - log_out:
-      type: file
-      description: STAR lot out file
-      pattern: "*Log.out"
-  - log_progress:
-      type: file
-      description: STAR log progress file
-      pattern: "*Log.progress.out"
-  - version:
-      type: file
-      description: File containing software version
-      pattern: "*.{version.txt}"
-  - bam_sorted:
-      type: file
-      description: Sorted BAM file of read alignments (optional)
-      pattern: "*sortedByCoord.out.bam"
-  - bam_transcript:
-      type: file
-      description: Output BAM file of transcriptome alignment (optional)
-      pattern: "*toTranscriptome.out.bam"
-  - bam_unsorted:
-      type: file
-      description: Unsorted BAM file of read alignments (optional)
-      pattern: "*Aligned.unsort.out.bam"
-  - fastq:
-      type: file
-      description: Unmapped FastQ files (optional)
-      pattern: "*fastq.gz"
-  - tab:
-      type: file
-      description: STAR output tab file(s) (optional)
-      pattern: "*.tab"
-  - junction:
-      type: file
-      description: STAR chimeric junction output file (optional)
-      pattern: "*.out.junction"
-
-authors:
-  - "@kevinmenden"
-  - "@drpatelh"
-  - "@praveenraj2018"
diff --git a/modules/local/star/genomegenerate/main.nf b/modules/local/star/genomegenerate/main.nf
deleted file mode 100644
index 9f5b7786..00000000
--- a/modules/local/star/genomegenerate/main.nf
+++ /dev/null
@@ -1,37 +0,0 @@
-process STAR_GENOMEGENERATE {
-    tag "$fasta"
-    label 'process_high'
-
-    conda (params.enable_conda ? "bioconda::star=2.7.8a" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/star:2.7.8a--h9ee0642_1"
-    } else {
-        container "quay.io/biocontainers/star:2.7.8a--h9ee0642_1"
-    }
-
-    input:
-    path fasta
-    path gtf
-
-    output:
-    path "star/*"         , emit: index
-    path "versions.yml"   , emit: versions
-
-    script:
-    def memory   = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
-    def args = task.ext.args ?: ''
-    """
-    mkdir star
-    STAR \\
-        --runMode genomeGenerate \\
-        --genomeDir star/ \\
-        --genomeFastaFiles $fasta \\
-        --sjdbGTFfile $gtf \\
-        --sjdbOverhang ${params.read_length - 1} \\
-        --runThreadN $task.cpus \\
-        $memory \\
-        $args
-
-    STAR --version | sed -e "s/STAR_//g" > versions.yml
-    """
-}
diff --git a/modules/local/star/genomegenerate/meta.yml b/modules/local/star/genomegenerate/meta.yml
deleted file mode 100644
index aae0219b..00000000
--- a/modules/local/star/genomegenerate/meta.yml
+++ /dev/null
@@ -1,36 +0,0 @@
-name: star_genomegenerate
-description: Create index for STAR
-keywords:
-  - index
-  - fasta
-  - genome
-  - reference
-tools:
-  - star:
-      description: |
-        STAR is a software package for mapping DNA sequences against
-        a large reference genome, such as the human genome.
-      homepage: https://github.com/alexdobin/STAR
-      manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
-      doi: 10.1093/bioinformatics/bts635
-input:
-  - fasta:
-      type: file
-      description: Fasta file of the reference genome
-  - gtf:
-      type: file
-      description: GTF file of the reference genome
-
-output:
-  - index:
-      type: directory
-      description: Folder containing the star index files
-      pattern: "star"
-  - version:
-      type: file
-      description: File containing software version
-      pattern: "*.{version.txt}"
-
-authors:
-  - "@kevinmenden"
-  - "@drpatelh"
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index ffbf632f..a96228bb 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -4,13 +4,13 @@ process STARFUSION {
 
     conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/mulled-v2-57582e8bdbf51679bdcff9de91ae016a44e322de:b978a3a1e8715581329a267a2c9904574384180a-0"
+        container "docker.io/trinityctat/starfusion:1.10.1"
     } else {
-        container "quay.io/biocontainers/mulled-v2-57582e8bdbf51679bdcff9de91ae016a44e322de:b978a3a1e8715581329a267a2c9904574384180a-0"
+        container "docker.io/trinityctat/starfusion:1.10.1"
     }
 
     input:
-    tuple val(meta), path(reads)
+    tuple val(meta), path(reads), path(junction)
     path reference
 
     output:
@@ -20,22 +20,18 @@ process STARFUSION {
     path "versions.yml"                              , emit: versions
 
     script:
-    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def fasta = params.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
     def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
     """
-    STAR \\
-        --genomeDir $index \\
-        --readFilesIn $reads \\
-        --runThreadN $task.cpus \\
-        $args
-
     STAR-Fusion \\
         --genome_lib_dir $reference \\
-        $reads \\
+        $fasta \\
+        -J $junction \\
         --CPU $task.cpus \\
+        --examine_coding_effect \\
         --output_dir . \\
-        $args2
+        $args
 
     mv star-fusion.fusion_predictions.tsv ${prefix}.starfusion.fusion_predictions.tsv
     mv star-fusion.fusion_predictions.abridged.tsv ${prefix}.starfusion.abridged.tsv
@@ -43,4 +39,15 @@ process STARFUSION {
 
     echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > versions.yml
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.starfusion.fusion_predictions.tsv
+    touch ${prefix}.starfusion.abridged.tsv
+    touch ${prefix}.starfusion.abridged.coding_effect.tsv
+    touch versions.yml
+    """
 }
+
+
diff --git a/modules/local/starfusion/detect/meta.yml b/modules/local/starfusion/detect/meta.yml
index 2c38cd1a..098c6596 100644
--- a/modules/local/starfusion/detect/meta.yml
+++ b/modules/local/starfusion/detect/meta.yml
@@ -17,14 +17,13 @@ input:
       description: |
         Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
+  - genome_lib:
+      type: path
+      description: STAR-fusion reference genome lib folder
   - junction:
       type: file
       description: Chimeric junction output from STAR aligner
       pattern: "*.{out.junction}"
-  - genome_lib:
-      type: file
-      description: STAR-fusion reference genome lib folder
-      pattern: "*.*"
 
 output:
   - meta:
@@ -35,11 +34,19 @@ output:
   - version:
       type: file
       description: File containing software version
-      pattern: "*.{version.txt}"
+      pattern: "*.{versions.yml}"
   - fusions:
       type: file
       description: Fusion events from STAR-fusion
       pattern: "*.{fusion_predictions.tsv}"
+  - abridged:
+      type: file
+      description: Fusion events from STAR-fusion
+      pattern: "*.{fusion.abridged.tsv}"
+  - coding_effect:
+      type: file
+      description: Fusion events from STAR-fusion
+      pattern: "*.{coding_effect.tsv}"
 
 authors:
   - "@praveenraj2018"
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 432d5b6d..119c33fd 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -8,6 +8,7 @@ include { ARRIBA }                           from '../../modules/nf-core/modules
 
 workflow ARRIBA_WORKFLOW {
     take:
+        reads
         fasta
         index
         gtf
@@ -19,9 +20,11 @@ workflow ARRIBA_WORKFLOW {
         seq_platform = false
         seq_center = false
 
-        STAR_FOR_ARRIBA( fasta, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+        STAR_FOR_ARRIBA( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
         ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
 
+        // reads_align = reads.join(STAR_FOR_STARFUSION.out.junction )
+
         ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
         ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf
deleted file mode 100644
index 52f628b2..00000000
--- a/subworkflows/local/prepare_genome.nf
+++ /dev/null
@@ -1,91 +0,0 @@
-//
-// Uncompress and prepare reference genome files
-//
-
-params.genome_options                   = [:]
-params.star_index_options               = [:]
-params.starfusion_untar_options         = [:]
-params.starfusion_download_options      = [:]
-params.fusioncatcher_download_options   = [:]
-
-include { GUNZIP as GUNZIP_FASTA                } from '../../modules/nf-core/modules/gunzip/main'                  addParams( options: params.genome_options )
-include { UNTAR as UNTAR_STAR_INDEX             } from '../../modules/nf-core/modules/untar/main'                   addParams( options: params.star_index_options )
-include { UNTAR as UNTAR_STARFUSION_GENOME      } from '../../modules/nf-core/modules/untar/main'                   addParams( options: params.starfusion_untar_options )
-include { SAMTOOLS_FAIDX                        } from '../../modules/nf-core/modules/samtools/faidx/main'          addParams( options: params.genome_options )
-include { STAR_GENOMEGENERATE                   } from '../../modules/nf-core/modules/star/genomegenerate/main'     addParams( options: params.star_index_options )
-include { STARFUSION_DOWNLOADGENOME             } from '../../modules/local/starfusion/download/main'               addParams( options: params.starfusion_download_options)
-include { FUSIONCATCHER_DOWNLOADGENOME          } from '../../modules/local/fusioncatcher/download/main'            addParams( options: params.fusioncatcher_download_options)
-
-workflow PREPARE_GENOME {
-
-    take:
-    prepare_tool_indices
-
-    main:
-
-    // Uncompress genome fasta file if required
-    ch_fasta = params.fasta.endsWith('.gz') ? GUNZIP_FASTA ( params.fasta ).gunzipe : file(params.fasta)
-
-    // Index the genome fasta
-    ch_fasta_fai = Channel.empty()
-    ch_fasta_fai = params.fasta_fai ? file(params.fasta_fai) : SAMTOOLS_FAIDX(ch_fasta).fai
-
-    // Get the GTF file
-    if (params.gtf) {
-        ch_gtf = params.gtf.endsWith('.gz') ? GUNZIP_GTF ( params.gtf ).gunzip : file(params.gtf)
-    }
-
-    // Uncompress STAR index or generate from scratch if required
-    ch_star_index   = Channel.empty()
-    ch_star_version = Channel.empty()
-    if ('star' in prepare_tool_indices) {
-        if (params.star_index) {
-            ch_star_index = params.star_index.endsWith('.tar.gz') ? UNTAR_STAR_INDEX ( params.star_index ).untar : file(params.star_index)
-        } else {
-            ch_star_index   = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index
-            ch_star_version = STAR_GENOMEGENERATE.out.version
-        }
-    }
-
-    //Check genome data required for STAR-Fusion
-    ch_starfusion_resource = Channel.empty()
-    if (params.starfusion){
-        if (params.starfusion_genome) {
-            ch_sf_genome = params.starfusion_genome
-            if (ch_sf_genome.endsWith('.tar.gz')){
-                root_path = UNTAR_STARFUSION_GENOME (ch_sf_genome).untar
-                ch_starfusion_resource = file("${root_path}/ctat_genome_lib_build_dir")
-            }
-            else {
-                ch_starfusion_resource = file(ch_sf_genome)
-            }
-        }
-        else{
-            ch_starfusion_resource = STARFUSION_DOWNLOADGENOME (params.genome).out.reference
-        }
-    }
-
-    //Check genome data required for FusionCatcher
-    ch_fusioncatcher_resource = Channel.empty()
-    if (params.fusioncatcher){
-        if (params.fusioncatcher_genome){
-            ch_fusioncatcher_resource = file(params.fusioncatcher_genome)
-        }
-        else{
-            ch_fusioncatcher_resource = FUSIONCATCHER_DOWNLOADGENOME (params.organism).out.reference
-        }
-    }
-
-    emit:
-    fasta                   = ch_fasta                  // path: genome.fasta
-    fai                     = ch_fasta_fai              // path: genome.fasta.fai
-    gtf                     = ch_gtf                    // path: genome.gtf
-    star_index              = ch_star_index             // path: star/index/
-    starfusion_resource     = ch_starfusion_resource    // path: starfusion genome
-    fusioncatcher_resource  = ch_fusioncatcher_resource // path: fusioncatcher genome
-
-
-}
-
-
-
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index a0415d58..061b10d1 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -25,7 +25,6 @@ for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true
 
 // Check mandatory parameters
 if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' }
-// if (!params.build_references && !params.genomes_base) { exit 1 }
 
 
 /*
@@ -47,15 +46,13 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
 include { INPUT_CHECK                   } from '../subworkflows/local/input_check'
-// include { ARRIBA                        } from '../modules/local/arriba/detect/main'
-// include { PREPARE_GENOME                } from '../subworkflows/local/prepare_genome'
 
-// include { STARFUSION                 }   from '../modules/local/starfusion/detection/main'
 include { FUSIONCATCHER                 }   from '../modules/local/fusioncatcher/detect/main'
-include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
+// include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
 
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
+// include { PIZZLY_WORKFLOW               }   from '../subworkflows/local/pizzly_workflow'
 
 /*
 ========================================================================================
@@ -97,50 +94,34 @@ workflow RNAFUSION {
     //
     // MODULE: Run FastQC
     //
-    // FASTQC (
-    //     INPUT_CHECK.out.reads
-    // )
-    // ch_versions = ch_versions.mix(FASTQC.out.versions.first())
+    FASTQC (
+        INPUT_CHECK.out.reads
+    )
+    ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
-    // CUSTOM_DUMPSOFTWAREVERSIONS (
-    //     ch_versions.unique().collectFile(name: 'collated_versions.yml')
-    // )
+    CUSTOM_DUMPSOFTWAREVERSIONS (
+        ch_versions.unique().collectFile(name: 'collated_versions.yml')
+    )
 
     //
     // MODULE: MultiQC
     //
-    // workflow_summary    = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params)
-    // ch_workflow_summary = Channel.value(workflow_summary)
-
-    // ch_multiqc_files = Channel.empty()
-    // ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
-    // ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
-    // ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    // ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
-    // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
-
-    // MULTIQC (
-    //     ch_multiqc_files.collect()
-    // )
-
-    // multiqc_report       = MULTIQC.out.report.toList()
-    // ch_versions          = ch_versions.mix(MULTIQC.out.versions)
-
-
-
-    if (params.ericscript){
-        // def checkPathParamList = []
-        // for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
-
-        ERICSCRIPT (
-            INPUT_CHECK.out.reads,
-            params.ericscript_ref
-        )
-    }
-
-
-
+    workflow_summary    = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params)
+    ch_workflow_summary = Channel.value(workflow_summary)
+
+    ch_multiqc_files = Channel.empty()
+    ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+
+    MULTIQC (
+        ch_multiqc_files.collect()
+    )
 
+    multiqc_report       = MULTIQC.out.report.toList()
+    ch_versions          = ch_versions.mix(MULTIQC.out.versions)
 
 
 
@@ -150,13 +131,23 @@ workflow RNAFUSION {
 
         ARRIBA_WORKFLOW (
             INPUT_CHECK.out.reads,
+            params.fasta,
             params.starindex_ref,
             gtf
         )
     }
 
+    // // Run pizzly/kallisto
+    // if (params.pizzly){
+    //     index ="${params.pizzly_ref}/kallisto.idx"
+    //     PIZZLY_WORKFLOW (
+    //         INPUT_CHECK.out.reads,
+    //         index
+    //     )
+    // }
+
 
-    // Run ericscript
+    // // Run ericscript
     // if (params.ericscript){
     //     ERICSCRIPT (
     //         INPUT_CHECK.out.reads,
@@ -168,20 +159,22 @@ workflow RNAFUSION {
     //Run STAR fusion
     if (params.starfusion){
         gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
+        index ="${params.starfusion_ref}/ctat_genome_lib_build_dir"
+
         STARFUSION_WORKFLOW (
             INPUT_CHECK.out.reads,
             params.starindex_ref,
             gtf,
-            params.starfusion_ref
-        )
-    }
-    //Run FusionCatcher
-    if (params.fusioncatcher){
-        FUSIONCATCHER (
-            INPUT_CHECK.out.reads,
-            params.fusioncatcher_ref
+            index
         )
     }
+    // //Run FusionCatcher
+    // if (params.fusioncatcher){
+    //     FUSIONCATCHER (
+    //         INPUT_CHECK.out.reads,
+    //         params.fusioncatcher_ref
+    //     )
+    // }
 }
 
 /*

From 22a6445d2055cb2b5a6f3515f8003000a73d901d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 17:21:50 +0100
Subject: [PATCH 133/359] reference folders don't have to exist to exist as
 parameter

---
 workflows/rnafusion.nf | 6 ++----
 1 file changed, 2 insertions(+), 4 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 061b10d1..2ff43b01 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -13,15 +13,13 @@ WorkflowRnafusion.initialise(params, log)
 // Check input path parameters to see if they exist
 
 
-//TODO problem here as all the folders have to exist
 def checkPathParamList = [
-    params.input, params.multiqc_config, params.fasta,
-    params.genomes_base,
+    params.input, params.multiqc_config,
+    params.fasta, params.genomes_base,
     params.fusioncatcher_ref, params.starfusion_ref,
     params.arriba_ref, params.ericscript_ref
 ]
 
-for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
 
 // Check mandatory parameters
 if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' }

From f25e28325ca6a8abbfff20a512bdecb774942fea Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Feb 2022 18:02:30 +0100
Subject: [PATCH 134/359] pizzly and kallisto start

---
 modules/local/kallisto/quant/main.nf  | 38 +++++++++++++++++++++++++++
 modules/local/pizzly/detect/main.nf   | 12 +++------
 subworkflows/local/pizzly_workflow.nf | 31 ++++++++++++++++++++++
 subworkflows/local/squid_workflow.nf  | 34 ++++++++++++++++++++++++
 4 files changed, 107 insertions(+), 8 deletions(-)
 create mode 100644 modules/local/kallisto/quant/main.nf
 create mode 100644 subworkflows/local/pizzly_workflow.nf
 create mode 100644 subworkflows/local/squid_workflow.nf

diff --git a/modules/local/kallisto/quant/main.nf b/modules/local/kallisto/quant/main.nf
new file mode 100644
index 00000000..c02e938a
--- /dev/null
+++ b/modules/local/kallisto/quant/main.nf
@@ -0,0 +1,38 @@
+process KALLISTO_QUANT {
+    tag "kalliso_quant"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1' :
+        'quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1' }"
+
+
+    input:
+    tuple val(meta), path(reads)
+    path index
+
+    output:
+    path "versions.yml"                        , emit: versions
+    tuple val(meta), path("fusion.txt")        , emit: txt
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    kallisto quant \
+        -t $task.cpus \
+        -i $index \
+        --fusion \
+        -o . \
+        $reads
+
+    touch versions.yml
+
+    """
+}
+    // cat <<-END_VERSIONS > versions.yml
+    // "${task.process}":
+    //     kallisto: \$(kallisto | sed 's/^kallisto //; s/Usage.*\$//')
+    // END_VERSIONS
+    // echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > versions.yml
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index 790987ba..f375e5ec 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -4,18 +4,15 @@ process PIZZLY {
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2 bioconda::pizzly==0.37.3" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        // FIX: create a multicontainer image
         container "https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1"
     } else {
-        // FIX: create a multicontainer image
         container "quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1"
     }
 
     input:
-    tuple val(meta), path(reads)
-    path gtf
-    path index
-    path transcript
+    tuple val(meta), path(txt)
+    transcript
+    gtf
 
     output:
     path "versions.yml"                                     , emit: versions
@@ -24,9 +21,8 @@ process PIZZLY {
 
     script:
     def args = task.ext.args ?: ''
-    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+    def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    kallisto quant -t $task.cpus -i $index --fusion -o output $reads
     pizzly \\
         $args \\
         --gtf $gtf \\
diff --git a/subworkflows/local/pizzly_workflow.nf b/subworkflows/local/pizzly_workflow.nf
new file mode 100644
index 00000000..69433dda
--- /dev/null
+++ b/subworkflows/local/pizzly_workflow.nf
@@ -0,0 +1,31 @@
+//
+// Check input samplesheet and get read channels
+//
+
+include { KALLISTO_QUANT }     from '../../modules/local/kallisto/quant/main'
+// include { ARRIBA }                           from '../../modules/nf-core/modules/arriba/main'
+
+
+workflow PIZZLY_WORKFLOW {
+    take:
+        fasta
+        index
+        transcript
+        gtf
+
+    main:
+        ch_versions = Channel.empty()
+
+        KALLISTO_QUANT( fasta, index, gtf,)
+        // ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
+
+        // ARRIBA ( STAR_F/OR_ARRIBA.out.bam, fasta, gtf )
+        // ch_versions = ch_versions.mix(ARRIBA.out.versions)
+
+
+    emit:
+        KALLISTO_QUANT.out.txt
+        // ARRIBA.out.fusions_fail
+        // versions = ch_versions.ifEmpty(null)
+    }
+
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
new file mode 100644
index 00000000..4f513632
--- /dev/null
+++ b/subworkflows/local/squid_workflow.nf
@@ -0,0 +1,34 @@
+//
+// Check input samplesheet and get read channels
+//
+
+include { STAR_ALIGN as STAR_FOR_SQUID }    from '../../modules/nf-core/modules/star/align/main'
+// include { ARRIBA }                           from '../../modules/nf-core/modules/arriba/main'
+
+
+workflow SQUID_WORKFLOW {
+    take:
+        fasta
+        index
+        gtf
+
+    main:
+        ch_versions = Channel.empty()
+
+        star_ignore_sjdbgtf = false
+        seq_platform = false
+        seq_center = false
+
+        STAR_FOR_SQUID( fasta, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+        ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
+
+        ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
+        ch_versions = ch_versions.mix(ARRIBA.out.versions)
+
+
+    emit:
+        ARRIBA.out.fusions
+        ARRIBA.out.fusions_fail
+        versions = ch_versions.ifEmpty(null)
+    }
+

From 00c0abf7670ac399e298d04a232aeaf273ca144c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 9 Feb 2022 10:39:22 +0100
Subject: [PATCH 135/359] add pizzly/kallisto workflow

---
 conf/modules.config                   |  5 +++--
 modules/local/kallisto/quant/main.nf  | 12 +++++------
 modules/local/pizzly/detect/main.nf   | 23 +++++++++++---------
 subworkflows/local/pizzly_workflow.nf | 16 +++++++-------
 workflows/rnafusion.nf                | 30 ++++++++++++++++-----------
 5 files changed, 48 insertions(+), 38 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 86ab19f1..957e7030 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -165,11 +165,12 @@ process {
     // }
 
 
-/*
+
     withName: PIZZLY {
-        ext.args = = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
+        ext.args = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
     }
 
+/*
     withName: STARFUSION {
         ext.args  = "--chimSegmentMin 12 --chimJunctionOverhangMin 8 --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignIntronMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --outSAMattrRGline ID:GRPundef --chimMultimapScoreRange 3 --chimScoreJunctionNonGTAG -4 --chimMultimapNmax 20 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLminOverLmate 0 --alignSplicedMateMapLmin 30"
         ext.args2 = "--examine_coding_effect -J Chimeric.out.junction"
diff --git a/modules/local/kallisto/quant/main.nf b/modules/local/kallisto/quant/main.nf
index c02e938a..8c7fac5c 100644
--- a/modules/local/kallisto/quant/main.nf
+++ b/modules/local/kallisto/quant/main.nf
@@ -27,12 +27,10 @@ process KALLISTO_QUANT {
         -o . \
         $reads
 
-    touch versions.yml
-
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        kallisto: \$(echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//')
+    END_VERSIONS
     """
 }
-    // cat <<-END_VERSIONS > versions.yml
-    // "${task.process}":
-    //     kallisto: \$(kallisto | sed 's/^kallisto //; s/Usage.*\$//')
-    // END_VERSIONS
-    // echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > versions.yml
+
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index f375e5ec..d17b02c1 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -1,23 +1,23 @@
 process PIZZLY {
-    tag "pizzly"
+    tag "$meta.id"
     label 'process_medium'
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2 bioconda::pizzly==0.37.3" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1"
+        container "https://depot.galaxyproject.org/singularity/pizzly:0.37.3--py36_2"
     } else {
         container "quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1"
     }
 
     input:
     tuple val(meta), path(txt)
-    transcript
-    gtf
+    path transcript
+    path gtf
 
     output:
-    path "versions.yml"                                     , emit: versions
-    tuple val(meta), path "${prefix}.pizzly.txt"            , emit: fusions
-    tuple val(meta), path "${prefix}.pizzly.unfiltered.json", emit: fusions_unfiltered
+    path "versions.yml"                              , emit: versions
+    tuple val(meta), path('*_pizzly.txt')            , emit: fusions
+    tuple val(meta), path('*unfiltered.json')        , emit: fusions_unfiltered
 
     script:
     def args = task.ext.args ?: ''
@@ -27,10 +27,13 @@ process PIZZLY {
         $args \\
         --gtf $gtf \\
         --fasta $transcript \\
-        --output ${prefix}.pizzly output/fusion.txt
+        --output ${prefix}_pizzly $txt
 
-    pizzly_flatten_json.py ${prefix}.pizzly.json ${prefix}.pizzly.txt
+    pizzly_flatten_json.py ${prefix}_pizzly.json ${prefix}_pizzly.txt
 
-    echo \$(kallisto 2>&1) | sed 's/^kallisto //; s/Usage.*\$//' > versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        pizzly: \$(pizzly --version | grep pizzly | sed -e "s/pizzly version: //g")
+    END_VERSIONS
     """
 }
diff --git a/subworkflows/local/pizzly_workflow.nf b/subworkflows/local/pizzly_workflow.nf
index 69433dda..98286288 100644
--- a/subworkflows/local/pizzly_workflow.nf
+++ b/subworkflows/local/pizzly_workflow.nf
@@ -2,24 +2,26 @@
 // Check input samplesheet and get read channels
 //
 
-include { KALLISTO_QUANT }     from '../../modules/local/kallisto/quant/main'
-// include { ARRIBA }                           from '../../modules/nf-core/modules/arriba/main'
+include { KALLISTO_QUANT    }     from '../../modules/local/kallisto/quant/main'
+include { PIZZLY            }     from '../../modules/local/pizzly/detect/main'
 
 
 workflow PIZZLY_WORKFLOW {
     take:
-        fasta
+        reads
         index
-        transcript
         gtf
+        transcript
 
     main:
         ch_versions = Channel.empty()
 
-        KALLISTO_QUANT( fasta, index, gtf,)
-        // ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
+        KALLISTO_QUANT( reads, index )
+        ch_versions = ch_versions.mix(KALLISTO_QUANT.out.versions)
+
+
 
-        // ARRIBA ( STAR_F/OR_ARRIBA.out.bam, fasta, gtf )
+        PIZZLY( KALLISTO_QUANT.out.txt, transcript, gtf )
         // ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2ff43b01..87795b07 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -16,8 +16,10 @@ WorkflowRnafusion.initialise(params, log)
 def checkPathParamList = [
     params.input, params.multiqc_config,
     params.fasta, params.genomes_base,
+    params.ensembl_ref,
     params.fusioncatcher_ref, params.starfusion_ref,
-    params.arriba_ref, params.ericscript_ref
+    params.arriba_ref, params.ericscript_ref,
+    params.ensembl_version
 ]
 
 
@@ -46,11 +48,11 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 include { INPUT_CHECK                   } from '../subworkflows/local/input_check'
 
 include { FUSIONCATCHER                 }   from '../modules/local/fusioncatcher/detect/main'
-// include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
+include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
 
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
-// include { PIZZLY_WORKFLOW               }   from '../subworkflows/local/pizzly_workflow'
+include { PIZZLY_WORKFLOW               }   from '../subworkflows/local/pizzly_workflow'
 
 /*
 ========================================================================================
@@ -122,7 +124,6 @@ workflow RNAFUSION {
     ch_versions          = ch_versions.mix(MULTIQC.out.versions)
 
 
-
     // Run STAR alignment and Arriba
     if (params.arriba){
         gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
@@ -135,14 +136,19 @@ workflow RNAFUSION {
         )
     }
 
-    // // Run pizzly/kallisto
-    // if (params.pizzly){
-    //     index ="${params.pizzly_ref}/kallisto.idx"
-    //     PIZZLY_WORKFLOW (
-    //         INPUT_CHECK.out.reads,
-    //         index
-    //     )
-    // }
+    // Run pizzly/kallisto
+    if (params.pizzly){
+        index ="${params.pizzly_ref}/kallisto"
+        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+        transcript ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
+
+        PIZZLY_WORKFLOW (
+            INPUT_CHECK.out.reads,
+            index,
+            gtf,
+            transcript
+        )
+    }
 
 
     // // Run ericscript

From 0e497a04a5f6265a7e50e162476ec406b97d696f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 9 Feb 2022 22:28:15 +0100
Subject: [PATCH 136/359] initial squid commit

---
 conf/modules.config                           | 22 +++++-----
 modules.json                                  |  3 ++
 modules/local/squid/main.nf                   | 30 +++++++++++++
 modules/nf-core/modules/samtools/sort/main.nf | 31 +++++++++++++
 .../nf-core/modules/samtools/sort/meta.yml    | 44 +++++++++++++++++++
 subworkflows/local/squid_workflow.nf          | 19 +++++---
 6 files changed, 131 insertions(+), 18 deletions(-)
 create mode 100644 modules/local/squid/main.nf
 create mode 100644 modules/nf-core/modules/samtools/sort/main.nf
 create mode 100644 modules/nf-core/modules/samtools/sort/meta.yml

diff --git a/conf/modules.config b/conf/modules.config
index 957e7030..0115d2d0 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -152,17 +152,17 @@ process {
     }
 
 
-    //     withName: STAR_FOR_SQUID {
-    //     ext.args = '--twopassMode Basic \
-    //     --chimOutType SeparateSAMold \
-    //     --chimSegmentMin 20 \
-    //     --chimJunctionOverhangMin 12 \
-    //     --alignSJDBoverhangMin 10 \
-    //     --outReadsUnmapped Fastx \
-    //     --outSAMstrandField intronMotif \
-    //     --outSAMtype BAM SortedByCoordinate \
-    //     --readFilesCommand zcat'
-    // }
+        withName: STAR_FOR_SQUID {
+        ext.args = '--twopassMode Basic \
+        --chimOutType SeparateSAMold \
+        --chimSegmentMin 20 \
+        --chimJunctionOverhangMin 12 \
+        --alignSJDBoverhangMin 10 \
+        --outReadsUnmapped Fastx \
+        --outSAMstrandField intronMotif \
+        --outSAMtype BAM SortedByCoordinate \
+        --readFilesCommand zcat'
+    }
 
 
 
diff --git a/modules.json b/modules.json
index 8c0ca267..05560d1d 100644
--- a/modules.json
+++ b/modules.json
@@ -21,6 +21,9 @@
             "samtools/faidx": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
+            "samtools/sort": {
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+            },
             "star/align": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
diff --git a/modules/local/squid/main.nf b/modules/local/squid/main.nf
new file mode 100644
index 00000000..48eb5b79
--- /dev/null
+++ b/modules/local/squid/main.nf
@@ -0,0 +1,30 @@
+
+process SQUID {
+    tag "squid"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::squid=1.5" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/squid:1.5--haac9b31_4' :
+        'https://depot.galaxyproject.org/singularity/squid:1.5--haac9b31_4' }"
+
+
+
+    input:
+    tuple val(meta), path(bam)
+    path gtf
+
+    output:
+    tuple val(meta), path("*_sv.txt"), emit: fusions
+    tuple val(meta), path("*_annotated.txt"), emit: fusions_annotated
+    path  "versions.yml"          , emit: versions
+
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    squid -b $bam -o ${prefix}_fusions
+    AnnotateSQUIDOutput.py $gtf ${prefix}_fusions_sv.txt ${prefix}_fusions_annotated.txt
+    """
+}
diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf
new file mode 100644
index 00000000..0f2237cc
--- /dev/null
+++ b/modules/nf-core/modules/samtools/sort/main.nf
@@ -0,0 +1,31 @@
+process SAMTOOLS_SORT {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::samtools=1.14" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' :
+        'quay.io/biocontainers/samtools:1.14--hb421002_0' }"
+
+    input:
+    tuple val(meta), path(bam)
+
+    output:
+    tuple val(meta), path("*.bam"), emit: bam
+    path  "versions.yml"          , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    samtools sort $args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/samtools/sort/meta.yml b/modules/nf-core/modules/samtools/sort/meta.yml
new file mode 100644
index 00000000..3402a068
--- /dev/null
+++ b/modules/nf-core/modules/samtools/sort/meta.yml
@@ -0,0 +1,44 @@
+name: samtools_sort
+description: Sort SAM/BAM/CRAM file
+keywords:
+    - sort
+    - bam
+    - sam
+    - cram
+tools:
+    - samtools:
+        description: |
+            SAMtools is a set of utilities for interacting with and post-processing
+            short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+            These files are generated as output by short read aligners like BWA.
+        homepage: http://www.htslib.org/
+        documentation: hhttp://www.htslib.org/doc/samtools.html
+        doi: 10.1093/bioinformatics/btp352
+        licence: ['MIT']
+input:
+    - meta:
+        type: map
+        description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+output:
+    - meta:
+        type: map
+        description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: Sorted BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+    - versions:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+authors:
+    - "@drpatelh"
+    - "@ewels"
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 4f513632..066184e7 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -3,11 +3,13 @@
 //
 
 include { STAR_ALIGN as STAR_FOR_SQUID }    from '../../modules/nf-core/modules/star/align/main'
-// include { ARRIBA }                           from '../../modules/nf-core/modules/arriba/main'
+include { SAMTOOLS_SORT }                   from '../../modules/nf-core/modules/samtools/sort/main'
+include { SQUID }                           from '../../modules/local/squid/main'
 
 
 workflow SQUID_WORKFLOW {
     take:
+        reads
         fasta
         index
         gtf
@@ -19,16 +21,19 @@ workflow SQUID_WORKFLOW {
         seq_platform = false
         seq_center = false
 
-        STAR_FOR_SQUID( fasta, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
-        ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
+        STAR_FOR_SQUID( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+        ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions)
 
-        ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
-        ch_versions = ch_versions.mix(ARRIBA.out.versions)
+        SAMTOOLS_SORT ( STAR_FOR_SQUID.out.bam )
+        ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions)
+
+        SQUID ( SAMTOOLS_SORT.out.bam, gtf )
+        ch_versions = ch_versions.mix(SQUID.out.versions)
 
 
     emit:
-        ARRIBA.out.fusions
-        ARRIBA.out.fusions_fail
+        // ARRIBA.out.fusions
+        // ARRIBA.out.fusions_fail
         versions = ch_versions.ifEmpty(null)
     }
 

From 8fd025df3ff4740a0873e7a86c9773992cfe9d96 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 9 Feb 2022 22:33:23 +0100
Subject: [PATCH 137/359] fix liniting

---
 .github/workflows/linting.yml           | 12 +++---------
 docs/usage.md                           |  2 +-
 modules/local/ericscript/detect/main.nf |  6 +++---
 workflows/rnafusion.nf                  | 24 +++++++++++++++++++-----
 4 files changed, 26 insertions(+), 18 deletions(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 3b448773..faa54e71 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -12,9 +12,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - uses: actions/checkout@v2
-      - uses: actions/setup-node@v1
-        with:
-          node-version: '10'
+      - uses: actions/setup-node@v2
       - name: Install markdownlint
         run: npm install -g markdownlint-cli
       - name: Run Markdownlint
@@ -51,9 +49,7 @@ jobs:
     steps:
       - uses: actions/checkout@v2
 
-      - uses: actions/setup-node@v1
-        with:
-          node-version: '10'
+      - uses: actions/setup-node@v2
 
       - name: Install editorconfig-checker
         run: npm install -g editorconfig-checker
@@ -65,9 +61,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - uses: actions/checkout@v1
-      - uses: actions/setup-node@v1
-        with:
-          node-version: '10'
+      - uses: actions/setup-node@v2
       - name: Install yaml-lint
         run: npm install -g yaml-lint
       - name: Run yaml-lint
diff --git a/docs/usage.md b/docs/usage.md
index 6ce66675..aa165a7d 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -308,7 +308,7 @@ Work dir:
 Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
 ```
 
-To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
+To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
 
 ```nextflow
 process {
diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
index 57b685c7..43cca9d7 100644
--- a/modules/local/ericscript/detect/main.nf
+++ b/modules/local/ericscript/detect/main.nf
@@ -1,10 +1,10 @@
 process ERICSCRIPT {
     tag "eriscript"
-    label 'process_medium'
+    label 'process_low'
 
-    conda (params.enable_conda ? "bioconda::ericscript=0.5.5" : null)
+    conda (params.enable_conda ? "bioconda::ericscript=0.5.5 conda-forge::ncurses=6.1" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/ericscript:0.5.5--pl5.22.0r3.4.1_1"
+        container "quay.io/biocontainers/ericscript:0.5.5--4"
     } else {
         container "quay.io/biocontainers/ericscript:0.5.5--hdfd78af_5"
     }
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 87795b07..8f14e4a2 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -45,14 +45,14 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 //
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
-include { INPUT_CHECK                   } from '../subworkflows/local/input_check'
-
+include { INPUT_CHECK                   }   from '../subworkflows/local/input_check'
+// include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
 include { FUSIONCATCHER                 }   from '../modules/local/fusioncatcher/detect/main'
-include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
 
-include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
 include { PIZZLY_WORKFLOW               }   from '../subworkflows/local/pizzly_workflow'
+include { SQUID_WORKFLOW                }   from '../subworkflows/local/squid_workflow'
+include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
 
 /*
 ========================================================================================
@@ -123,7 +123,6 @@ workflow RNAFUSION {
     multiqc_report       = MULTIQC.out.report.toList()
     ch_versions          = ch_versions.mix(MULTIQC.out.versions)
 
-
     // Run STAR alignment and Arriba
     if (params.arriba){
         gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
@@ -150,6 +149,21 @@ workflow RNAFUSION {
         )
     }
 
+    // Run squid
+    if (params.squid){
+        // index ="${params.pizzly_ref}/kallisto"
+        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+        // transcript ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
+
+        SQUID_WORKFLOW (
+            INPUT_CHECK.out.reads,
+            params.fasta,
+            params.starindex_ref,
+            gtf,
+        )
+    }
+
+
 
     // // Run ericscript
     // if (params.ericscript){

From ee931bdb5dbb59fdee88304aea4f1883227d4019 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 9 Feb 2022 22:36:28 +0100
Subject: [PATCH 138/359] fix markdownlinting

---
 .github/workflows/linting.yml | 12 +++---------
 docs/usage.md                 |  2 +-
 2 files changed, 4 insertions(+), 10 deletions(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 3b448773..faa54e71 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -12,9 +12,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - uses: actions/checkout@v2
-      - uses: actions/setup-node@v1
-        with:
-          node-version: '10'
+      - uses: actions/setup-node@v2
       - name: Install markdownlint
         run: npm install -g markdownlint-cli
       - name: Run Markdownlint
@@ -51,9 +49,7 @@ jobs:
     steps:
       - uses: actions/checkout@v2
 
-      - uses: actions/setup-node@v1
-        with:
-          node-version: '10'
+      - uses: actions/setup-node@v2
 
       - name: Install editorconfig-checker
         run: npm install -g editorconfig-checker
@@ -65,9 +61,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - uses: actions/checkout@v1
-      - uses: actions/setup-node@v1
-        with:
-          node-version: '10'
+      - uses: actions/setup-node@v2
       - name: Install yaml-lint
         run: npm install -g yaml-lint
       - name: Run yaml-lint
diff --git a/docs/usage.md b/docs/usage.md
index 6ce66675..aa165a7d 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -308,7 +308,7 @@ Work dir:
 Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
 ```
 
-To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
+To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
 
 ```nextflow
 process {

From a9f33bfa81b19cf65f4f6470d461b8f820553e97 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 10 Feb 2022 00:06:53 +0100
Subject: [PATCH 139/359] ignore file changed .github/workflows/linting.yml:
 TEMPORARY

---
 .nf-core.yml | 4 ++++
 1 file changed, 4 insertions(+)
 create mode 100644 .nf-core.yml

diff --git a/.nf-core.yml b/.nf-core.yml
new file mode 100644
index 00000000..b1370fd9
--- /dev/null
+++ b/.nf-core.yml
@@ -0,0 +1,4 @@
+lint:
+    files_unchanged:
+    # Added to ignore linting error: actions/setup-node@v1 until fixed release
+    - .github/workflows/linting.yml

From cedfc67568732bbc405fdb65118558204ce1acd0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 10 Feb 2022 09:47:48 +0100
Subject: [PATCH 140/359] Update modules/local/kallisto/quant/main.nf

typo fix

Co-authored-by: Alexander Peltzer <apeltzer@users.noreply.github.com>
---
 modules/local/kallisto/quant/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/kallisto/quant/main.nf b/modules/local/kallisto/quant/main.nf
index c02e938a..c422628b 100644
--- a/modules/local/kallisto/quant/main.nf
+++ b/modules/local/kallisto/quant/main.nf
@@ -1,5 +1,5 @@
 process KALLISTO_QUANT {
-    tag "kalliso_quant"
+    tag "kallisto_quant"
     label 'process_medium'
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)

From 1c7573ccc710521af28de9a0bdb41180a0a5766d Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Thu, 10 Feb 2022 11:47:48 +0100
Subject: [PATCH 141/359] Enable insecure download with wget for proxy users

---
 modules/local/starfusion/download/main.nf | 16 ++++++++--------
 1 file changed, 8 insertions(+), 8 deletions(-)

diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 16ba4ed0..db20d588 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -20,14 +20,14 @@ process STARFUSION_DOWNLOAD {
     """
     export TMPDIR=/tmp
 
-    wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz
-    wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz -O CTAT_HumanFusionLib_Mar2021.dat.gz
-    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p
+    wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
+    wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz -O CTAT_HumanFusionLib_Mar2021.dat.gz --no-check-certificate 
+    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p --no-check-certificate
 
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
 

From e68d006e89f29319a2fe393c3ac26c3860545bf2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Feb 2022 10:33:29 +0100
Subject: [PATCH 142/359] fixing squid

---
 conf/modules.config                           | 21 ++---
 modules.json                                  |  3 +
 modules/local/ericscript/detect/main.nf       |  4 +-
 modules/local/squid/{ => annotate}/main.nf    | 17 ++--
 modules/local/squid/detect/main.nf            | 32 +++++++
 modules/local/star/align/main.nf              | 73 ++++++++++++++++
 modules/local/star/align/meta.yml             | 85 +++++++++++++++++++
 modules/nf-core/modules/samtools/view/main.nf | 44 ++++++++++
 .../nf-core/modules/samtools/view/meta.yml    | 53 ++++++++++++
 subworkflows/local/squid_workflow.nf          | 22 +++--
 workflows/rnafusion.nf                        | 38 +++++----
 11 files changed, 344 insertions(+), 48 deletions(-)
 rename modules/local/squid/{ => annotate}/main.nf (55%)
 create mode 100644 modules/local/squid/detect/main.nf
 create mode 100644 modules/local/star/align/main.nf
 create mode 100644 modules/local/star/align/meta.yml
 create mode 100644 modules/nf-core/modules/samtools/view/main.nf
 create mode 100644 modules/nf-core/modules/samtools/view/meta.yml

diff --git a/conf/modules.config b/conf/modules.config
index 0115d2d0..e215c1d1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -71,7 +71,7 @@ process {
         mode: 'copy',
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
     ]
-}
+    }
     withName: STAR_GENOMEGENERATE {
         ext.args = "--sjdbOverhang ${params.read_length - 1}"
         publishDir = [
@@ -151,8 +151,7 @@ process {
         --chimMultimapNmax 50'
     }
 
-
-        withName: STAR_FOR_SQUID {
+    withName: STAR_FOR_SQUID {
         ext.args = '--twopassMode Basic \
         --chimOutType SeparateSAMold \
         --chimSegmentMin 20 \
@@ -164,26 +163,18 @@ process {
         --readFilesCommand zcat'
     }
 
-
-
     withName: PIZZLY {
         ext.args = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
     }
 
-/*
-    withName: STARFUSION {
-        ext.args  = "--chimSegmentMin 12 --chimJunctionOverhangMin 8 --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignIntronMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --outSAMattrRGline ID:GRPundef --chimMultimapScoreRange 3 --chimScoreJunctionNonGTAG -4 --chimMultimapNmax 20 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLminOverLmate 0 --alignSplicedMateMapLmin 30"
-        ext.args2 = "--examine_coding_effect -J Chimeric.out.junction"
+    withName: SAMTOOLS_VIEW {
+        ext.args = { "-Sb -o ${meta.id}_chimeric.bam" }
     }
 
-    withName: ARRIBA {
-        ext.args  = "--outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50"
-        ext.args2 = "-b references/${params.genome}/arriba/blacklist_hg38_GRCh38_v2.1.0.tsv.gz -k references/${params.genome}/arriba/known_fusions_hg38_GRCh38_v2.1.0.tsv.gz -p references/${params.genome}/arriba/protein_domains_hg38_GRCh38_v2.1.0.gff3"
+    withName: SAMTOOLS_SORT {
+        ext.prefix = { "${meta.id}_chimeric_sorted" }
     }
 
-*/
-
-
 }
 
 
diff --git a/modules.json b/modules.json
index 05560d1d..e11cb973 100644
--- a/modules.json
+++ b/modules.json
@@ -24,6 +24,9 @@
             "samtools/sort": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
+            "samtools/view": {
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+            },
             "star/align": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
diff --git a/modules/local/ericscript/detect/main.nf b/modules/local/ericscript/detect/main.nf
index 43cca9d7..baeb5a62 100644
--- a/modules/local/ericscript/detect/main.nf
+++ b/modules/local/ericscript/detect/main.nf
@@ -4,9 +4,9 @@ process ERICSCRIPT {
 
     conda (params.enable_conda ? "bioconda::ericscript=0.5.5 conda-forge::ncurses=6.1" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "quay.io/biocontainers/ericscript:0.5.5--4"
+        container "docker.io/nfcore/rnafusion:ericscript_0.5.5"
     } else {
-        container "quay.io/biocontainers/ericscript:0.5.5--hdfd78af_5"
+        container "docker.io/nfcore/rnafusion:ericscript_0.5.5"
     }
 
     input:
diff --git a/modules/local/squid/main.nf b/modules/local/squid/annotate/main.nf
similarity index 55%
rename from modules/local/squid/main.nf
rename to modules/local/squid/annotate/main.nf
index 48eb5b79..9ce03506 100644
--- a/modules/local/squid/main.nf
+++ b/modules/local/squid/annotate/main.nf
@@ -1,21 +1,20 @@
 
-process SQUID {
+process SQUID_ANNOTATE {
     tag "squid"
     label 'process_medium'
 
     conda (params.enable_conda ? "bioconda::squid=1.5" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/squid:1.5--haac9b31_4' :
-        'https://depot.galaxyproject.org/singularity/squid:1.5--haac9b31_4' }"
+        'docker.io/nfcore/rnafusion:squid_1.5-star2.7.1a' :
+        'docker.io/nfcore/rnafusion:squid_1.5-star2.7.1a' }"
 
 
 
     input:
-    tuple val(meta), path(bam)
+    tuple val(meta), path(txt)
     path gtf
 
     output:
-    tuple val(meta), path("*_sv.txt"), emit: fusions
     tuple val(meta), path("*_annotated.txt"), emit: fusions_annotated
     path  "versions.yml"          , emit: versions
 
@@ -24,7 +23,11 @@ process SQUID {
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    squid -b $bam -o ${prefix}_fusions
-    AnnotateSQUIDOutput.py $gtf ${prefix}_fusions_sv.txt ${prefix}_fusions_annotated.txt
+    AnnotateSQUIDOutput.py $gtf $txt ${prefix}_fusions_annotated.txt
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        squid: \$(echo \$(squid --version 2>&1) | sed 's/v//')
+    END_VERSIONS
     """
 }
diff --git a/modules/local/squid/detect/main.nf b/modules/local/squid/detect/main.nf
new file mode 100644
index 00000000..896813c6
--- /dev/null
+++ b/modules/local/squid/detect/main.nf
@@ -0,0 +1,32 @@
+
+process SQUID {
+    tag "squid"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::squid=1.5" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'docker.io/nfcore/rnafusion:squid_1.5-star2.7.1a' :
+        'docker.io/nfcore/rnafusion:squid_1.5-star2.7.1a' }"
+
+
+
+    input:
+    tuple val(meta), path(bam), path(chimeric_bam)
+
+    output:
+    tuple val(meta), path("*_sv.txt"), emit: fusions
+    path  "versions.yml"          , emit: versions
+
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    squid -b $bam -c $chimeric_bam -o ${prefix}_fusions
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        squid: \$(echo \$(squid --version 2>&1) | sed 's/v//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/star/align/main.nf b/modules/local/star/align/main.nf
new file mode 100644
index 00000000..ea3868dd
--- /dev/null
+++ b/modules/local/star/align/main.nf
@@ -0,0 +1,73 @@
+process STAR_ALIGN {
+    tag "$meta.id"
+    label 'process_high'
+
+    // Note: 2.7X indices incompatible with AWS iGenomes.
+    conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0' :
+        'quay.io/biocontainers/star:2.7.9a--h9ee0642_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    path  index
+    path  gtf
+    val star_ignore_sjdbgtf
+    val seq_platform
+    val seq_center
+
+    output:
+    tuple val(meta), path('*d.out.bam')       , emit: bam
+    tuple val(meta), path('*Log.final.out')   , emit: log_final
+    tuple val(meta), path('*Log.out')         , emit: log_out
+    tuple val(meta), path('*Log.progress.out'), emit: log_progress
+    path  "versions.yml"                      , emit: versions
+
+    tuple val(meta), path('*sortedByCoord.out.bam')  , optional:true, emit: bam_sorted
+    tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript
+    tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted
+    tuple val(meta), path('*fastq.gz')               , optional:true, emit: fastq
+    tuple val(meta), path('*.tab')                   , optional:true, emit: tab
+    tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
+    tuple val(meta), path('*.out.sam')               , optional:true, emit: sam
+
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def ignore_gtf      = star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
+    def seq_platform    = seq_platform ? "'PL:$seq_platform'" : ""
+    def seq_center      = seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform "
+    def out_sam_type    = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
+    def mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
+    """
+    STAR \\
+        --genomeDir $index \\
+        --readFilesIn $reads  \\
+        --runThreadN $task.cpus \\
+        --outFileNamePrefix $prefix. \\
+        $out_sam_type \\
+        $ignore_gtf \\
+        $seq_center \\
+        $args
+
+    $mv_unsorted_bam
+
+    if [ -f ${prefix}.Unmapped.out.mate1 ]; then
+        mv ${prefix}.Unmapped.out.mate1 ${prefix}.unmapped_1.fastq
+        gzip ${prefix}.unmapped_1.fastq
+    fi
+    if [ -f ${prefix}.Unmapped.out.mate2 ]; then
+        mv ${prefix}.Unmapped.out.mate2 ${prefix}.unmapped_2.fastq
+        gzip ${prefix}.unmapped_2.fastq
+    fi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        star: \$(STAR --version | sed -e "s/STAR_//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/star/align/meta.yml b/modules/local/star/align/meta.yml
new file mode 100644
index 00000000..f34e7ab9
--- /dev/null
+++ b/modules/local/star/align/meta.yml
@@ -0,0 +1,85 @@
+name: star_align
+description: Align reads to a reference genome using STAR
+keywords:
+  - align
+  - fasta
+  - genome
+  - reference
+tools:
+  - star:
+      description: |
+        STAR is a software package for mapping DNA sequences against
+        a large reference genome, such as the human genome.
+      homepage: https://github.com/alexdobin/STAR
+      manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
+      doi: 10.1093/bioinformatics/bts635
+      licence: ['MIT']
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
+  - index:
+      type: directory
+      description: STAR genome index
+      pattern: "star"
+output:
+  - bam:
+      type: file
+      description: Output BAM file containing read alignments
+      pattern: "*.{bam}"
+  - log_final:
+      type: file
+      description: STAR final log file
+      pattern: "*Log.final.out"
+  - log_out:
+      type: file
+      description: STAR lot out file
+      pattern: "*Log.out"
+  - log_progress:
+      type: file
+      description: STAR log progress file
+      pattern: "*Log.progress.out"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - bam_sorted:
+      type: file
+      description: Sorted BAM file of read alignments (optional)
+      pattern: "*sortedByCoord.out.bam"
+  - bam_transcript:
+      type: file
+      description: Output BAM file of transcriptome alignment (optional)
+      pattern: "*toTranscriptome.out.bam"
+  - bam_unsorted:
+      type: file
+      description: Unsorted BAM file of read alignments (optional)
+      pattern: "*Aligned.unsort.out.bam"
+  - fastq:
+      type: file
+      description: Unmapped FastQ files (optional)
+      pattern: "*fastq.gz"
+  - tab:
+      type: file
+      description: STAR output tab file(s) (optional)
+      pattern: "*.tab"
+  - junction:
+      type: file
+      description: STAR chimeric junction output file (optional)
+      pattern: "*.out.junction"
+  - sam:
+      type: file
+      description: Output SAM file containing read alignments
+      pattern: "*.{sam}"
+
+authors:
+  - "@kevinmenden"
+  - "@drpatelh"
+  - "@praveenraj2018"
diff --git a/modules/nf-core/modules/samtools/view/main.nf b/modules/nf-core/modules/samtools/view/main.nf
new file mode 100644
index 00000000..aee21a4e
--- /dev/null
+++ b/modules/nf-core/modules/samtools/view/main.nf
@@ -0,0 +1,44 @@
+process SAMTOOLS_VIEW {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::samtools=1.14" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' :
+        'quay.io/biocontainers/samtools:1.14--hb421002_0' }"
+
+    input:
+    tuple val(meta), path(input)
+    path fasta
+
+    output:
+    tuple val(meta), path("*.bam") , emit: bam , optional: true
+    tuple val(meta), path("*.cram"), emit: cram, optional: true
+    path  "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def reference = fasta ? "--reference ${fasta} -C" : ""
+    def file_type = input.getExtension()
+    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    samtools \\
+        view \\
+        --threads ${task.cpus-1} \\
+        ${reference} \\
+        $args \\
+        $input \\
+        $args2 \\
+        > ${prefix}.${file_type}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/samtools/view/meta.yml b/modules/nf-core/modules/samtools/view/meta.yml
new file mode 100644
index 00000000..5604bfa7
--- /dev/null
+++ b/modules/nf-core/modules/samtools/view/meta.yml
@@ -0,0 +1,53 @@
+name: samtools_view
+description: filter/convert SAM/BAM/CRAM file
+keywords:
+  - view
+  - bam
+  - sam
+  - cram
+tools:
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: hhttp://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - input:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - fasta:
+      type: optional file
+      description: Reference file the CRAM was created with
+      pattern: "*.{fasta,fa}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: filtered/converted BAM/SAM file
+      pattern: "*.{bam,sam}"
+  - cram:
+      type: file
+      description: filtered/converted CRAM file
+      pattern: "*.cram"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
+  - "@joseespinosa"
+  - "@FriederikeHanssen"
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 066184e7..98dada05 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -2,9 +2,11 @@
 // Check input samplesheet and get read channels
 //
 
-include { STAR_ALIGN as STAR_FOR_SQUID }    from '../../modules/nf-core/modules/star/align/main'
+include { STAR_ALIGN as STAR_FOR_SQUID }    from '../../modules/local/star/align/main'
+include { SAMTOOLS_VIEW }                   from '../../modules/nf-core/modules/samtools/view/main'
 include { SAMTOOLS_SORT }                   from '../../modules/nf-core/modules/samtools/sort/main'
-include { SQUID }                           from '../../modules/local/squid/main'
+include { SQUID }                           from '../../modules/local/squid/detect/main'
+include { SQUID_ANNOTATE }                  from '../../modules/local/squid/annotate/main'
 
 
 workflow SQUID_WORKFLOW {
@@ -22,14 +24,22 @@ workflow SQUID_WORKFLOW {
         seq_center = false
 
         STAR_FOR_SQUID( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
-        ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions)
+        ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
-        SAMTOOLS_SORT ( STAR_FOR_SQUID.out.bam )
-        ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions)
+        SAMTOOLS_VIEW ( STAR_FOR_SQUID.out.sam, [] )
+        ch_versions = ch_versions.mix(SAMTOOLS_VIEW.out.versions )
 
-        SQUID ( SAMTOOLS_SORT.out.bam, gtf )
+        SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam )
+        ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions )
+
+        bam_sorted = STAR_FOR_SQUID.out.bam_sorted.join(SAMTOOLS_SORT.out.bam )
+
+        SQUID ( bam_sorted )
         ch_versions = ch_versions.mix(SQUID.out.versions)
 
+        SQUID_ANNOTATE ( SQUID.out.fusions, gtf )
+        ch_versions = ch_versions.mix(SQUID_ANNOTATE.out.versions)
+
 
     emit:
         // ARRIBA.out.fusions
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 8f14e4a2..28c23c98 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -46,7 +46,7 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
 include { INPUT_CHECK                   }   from '../subworkflows/local/input_check'
-// include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
+include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
 include { FUSIONCATCHER                 }   from '../modules/local/fusioncatcher/detect/main'
 
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
@@ -94,6 +94,7 @@ workflow RNAFUSION {
     //
     // MODULE: Run FastQC
     //
+
     FASTQC (
         INPUT_CHECK.out.reads
     )
@@ -123,6 +124,7 @@ workflow RNAFUSION {
     multiqc_report       = MULTIQC.out.report.toList()
     ch_versions          = ch_versions.mix(MULTIQC.out.versions)
 
+
     // Run STAR alignment and Arriba
     if (params.arriba){
         gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
@@ -151,27 +153,25 @@ workflow RNAFUSION {
 
     // Run squid
     if (params.squid){
-        // index ="${params.pizzly_ref}/kallisto"
         gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-        // transcript ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
 
         SQUID_WORKFLOW (
             INPUT_CHECK.out.reads,
             params.fasta,
             params.starindex_ref,
-            gtf,
+            gtf
         )
     }
 
 
 
-    // // Run ericscript
-    // if (params.ericscript){
-    //     ERICSCRIPT (
-    //         INPUT_CHECK.out.reads,
-    //         params.ericscript_ref
-    //     )
-    // }
+    // Run ericscript
+    if (params.ericscript){
+        ERICSCRIPT (
+            INPUT_CHECK.out.reads,
+            params.ericscript_ref
+        )
+    }
 
 
     //Run STAR fusion
@@ -186,13 +186,15 @@ workflow RNAFUSION {
             index
         )
     }
-    // //Run FusionCatcher
-    // if (params.fusioncatcher){
-    //     FUSIONCATCHER (
-    //         INPUT_CHECK.out.reads,
-    //         params.fusioncatcher_ref
-    //     )
-    // }
+
+
+    //Run FusionCatcher
+    if (params.fusioncatcher){
+        FUSIONCATCHER (
+            INPUT_CHECK.out.reads,
+            params.fusioncatcher_ref
+        )
+    }
 }
 
 /*

From c5cf91ece8ecec0b06916c81511c618a1aa509f6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Feb 2022 15:38:38 +0100
Subject: [PATCH 143/359] fix arriba reference output to publishDir without
 subdirectories

---
 modules/local/arriba/download/main.nf | 6 ++++--
 1 file changed, 4 insertions(+), 2 deletions(-)

diff --git a/modules/local/arriba/download/main.nf b/modules/local/arriba/download/main.nf
index d7620345..5a1b96af 100644
--- a/modules/local/arriba/download/main.nf
+++ b/modules/local/arriba/download/main.nf
@@ -10,14 +10,16 @@ process ARRIBA_DOWNLOAD {
     }
 
     output:
-    path "versions.yml"             , emit: versions
-    path "arriba_v2.1.0/database/*" , emit: reference
+    path "versions.yml"   , emit: versions
+    path "*"              , emit: reference
 
     script:
     """
     wget https://github.com/suhrig/arriba/releases/download/v2.1.0/arriba_v2.1.0.tar.gz -O arriba_v2.1.0.tar.gz
     tar -xzvf arriba_v2.1.0.tar.gz
     rm arriba_v2.1.0.tar.gz
+    mv arriba_v2.1.0/database/* .
+    rm -r arriba_v2.1.0
 
     echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
     """

From 14e698b6c9f61ac1da9b7fca8ba0b56e18a572c3 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Tue, 22 Feb 2022 16:14:13 +0100
Subject: [PATCH 144/359] Fix for paths to arriba gff3 and tsv files

---
 conf/modules.config | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 86ab19f1..5a06dbdc 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -61,8 +61,8 @@ process {
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
-        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_2018-11-04.tsv \
-        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_2019-07-05.gff3"
+        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv \
+        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
     }
 
     withName: ERICSCRIPT_DOWNLOAD {

From 1df66603518e277b3b37eb0817795c15ebea6c3c Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Tue, 22 Feb 2022 16:21:19 +0100
Subject: [PATCH 145/359] Change to use tsv.gz

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index 5a06dbdc..175ea28b 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -61,7 +61,7 @@ process {
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
-        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv \
+        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv.gz \
         -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
     }
 

From f42858e54531090603be06223905ff1693487905 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Feb 2022 16:22:25 +0100
Subject: [PATCH 146/359] update file names, gzip should be supported by arriba

---
 conf/modules.config | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 86ab19f1..175ea28b 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -61,8 +61,8 @@ process {
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
-        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_2018-11-04.tsv \
-        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_2019-07-05.gff3"
+        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv.gz \
+        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
     }
 
     withName: ERICSCRIPT_DOWNLOAD {

From cc64fdf00131123fc9e9e5474fe29de17912e40d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Feb 2022 10:01:13 +0100
Subject: [PATCH 147/359] add cosmic arguments to get fusion-report db,
 fusionreport_ref path and arriba_fusion path for optional manual feed-up of
 arriba fusion output

---
 conf/modules.config | 14 ++++++++++++++
 nextflow.config     |  6 ++++++
 2 files changed, 20 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index e215c1d1..ccdc90ab 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -47,6 +47,15 @@ process {
         ]
     }
 
+    withName: FUSIONREPORT_DOWNLOAD {
+        publishDir = [
+            path: { "${params.genomes_base}/fusion_report_db" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
+
     withName: ARRIBA_DOWNLOAD {
         publishDir = [
             path: { "${params.genomes_base}/arriba" },
@@ -63,6 +72,7 @@ process {
         ]
         ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_2018-11-04.tsv \
         -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_2019-07-05.gff3"
+        // ext.when = params.arriba
     }
 
     withName: ERICSCRIPT_DOWNLOAD {
@@ -85,6 +95,10 @@ process {
         ext.args = "--continue --tries=42"
     }
 
+    // withName: FUSIONCATCHER {
+        // ext.args = "--continue --tries=42"
+    // }
+
     withName: KALLISTO_INDEX {
         ext.args = '-k 31'
         publishDir = [
diff --git a/nextflow.config b/nextflow.config
index 552e4d8b..d14fc8ce 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -13,6 +13,8 @@ params {
     input                      = null
     fasta                      = null
     build_references           = false
+    cosmic_username            = null
+    cosmic_passwd              = null
 
     // MultiQC options
     multiqc_config             = null
@@ -51,8 +53,12 @@ params {
     squid_ref                     = "${params.genomes_base}/squid"
     starfusion_ref                = "${params.genomes_base}/starfusion"
     starindex_ref                 = "${params.genomes_base}/star"
+    fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
 
 
+    // Path to fusion outputs
+    arriba_fusions                = null
+
     // Boilerplate options
     outdir                     = './results'
     tracedir                   = "${params.outdir}/pipeline_info"

From e6eb0df16c2b83d279ab781e612d9ef52f70e10f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Feb 2022 10:09:11 +0100
Subject: [PATCH 148/359] corresponding input arguments in nextflow_schema

---
 nextflow_schema.json | 20 ++++++++++++++++++++
 1 file changed, 20 insertions(+)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 0a93a2b9..ced1f1dc 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -81,6 +81,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba references"
                 },
+                "arriba_fusions": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to arriba output"
+                },
                 "ensembl_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -108,6 +113,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
+                "fusionreport_ref": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to fusionreport references"
+                },
                 "pizzly": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
@@ -151,6 +161,16 @@
                     "type": "string",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starindex references"
+                },
+                "cosmic_username": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "COSMIC username"
+                },
+                "cosmic_passwd": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "COSMIC password"
                 }
             }
         },

From c87774c4b5a067b1674d5ebf0a0a8f973df240e6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Feb 2022 14:34:33 +0100
Subject: [PATCH 149/359] add fusionreport to build_references

---
 workflows/build_references.nf | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index fbbc75c9..ad9c3f33 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -8,6 +8,7 @@ include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'
 include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'
 include { ERICSCRIPT_DOWNLOAD }             from '../modules/local/ericscript/download/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
+include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/download/main'
 include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
 
 /*
@@ -53,6 +54,10 @@ workflow BUILD_REFERENCES {
         STARFUSION_DOWNLOAD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
     }
 
+    if (params.fusionreport || params.all) {
+        FUSIONREPORT_DOWNLOAD( params.cosmic_usr, params.cosmic_passwd )
+    }
+
 }
 
 /*

From f007c52d4c8c5d304c73b3b6101f5a52eb1c671d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Feb 2022 10:49:43 +0100
Subject: [PATCH 150/359] add preliminary fusion report files

---
 modules/local/fusionreport/detect/main.nf    | 28 +++++++++++++
 modules/local/fusionreport/detect/meta.yml   | 35 ++++++++++++++++
 modules/local/fusionreport/download/main.nf  | 26 ++++++++++++
 modules/local/fusionreport/download/meta.yml | 35 ++++++++++++++++
 subworkflows/local/fusionreport_workflow.nf  | 44 ++++++++++++++++++++
 5 files changed, 168 insertions(+)
 create mode 100644 modules/local/fusionreport/detect/main.nf
 create mode 100644 modules/local/fusionreport/detect/meta.yml
 create mode 100644 modules/local/fusionreport/download/main.nf
 create mode 100644 modules/local/fusionreport/download/meta.yml
 create mode 100644 subworkflows/local/fusionreport_workflow.nf

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
new file mode 100644
index 00000000..44a4c824
--- /dev/null
+++ b/modules/local/fusionreport/detect/main.nf
@@ -0,0 +1,28 @@
+process FUSIONREPORT {
+    tag 'fusionreport'
+    label 'process_medium'
+
+    // Note: 2.7X indices incompatible with AWS iGenomes.
+    conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'docker.io/rannickscilifelab/fusion-report:2.1.5' :
+        'docker.io/rannickscilifelab/fusion-report:2.1.5' }"
+
+
+    input:
+    tuple val(meta), path(reads)
+    path(fusionreport_ref)
+    file(arriba_tools)
+
+    output:
+    path "*"                , emit: reference
+    path "versions.yml"     , emit: versions
+
+    script:
+
+    """
+    fusion_report run $meta.id . $fusionreport_ref --arriba $arriba_tools --allow-multiple-gene-symbols
+
+    fusion_report --version > versions.yml
+    """
+}
diff --git a/modules/local/fusionreport/detect/meta.yml b/modules/local/fusionreport/detect/meta.yml
new file mode 100644
index 00000000..d4bab11a
--- /dev/null
+++ b/modules/local/fusionreport/detect/meta.yml
@@ -0,0 +1,35 @@
+name: fusionreport_download
+description: Build DB for fusionreport
+keywords:
+  - sort
+tools:
+  - fusioncatcher:
+      description: Build DB for fusionreport
+      homepage: https://github.com/ndaniel/fusioncatcher/
+      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
+      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
+      doi: "10.1101/011650"
+      licence: ['GPL v3']
+
+input:
+  - username:
+      type: value
+      description: Organism for which the data is downloaded from Ensembl database and built
+      pattern: "*"
+  - passwd:
+      type: value
+      description: Organism for which the data is downloaded from Ensembl database and built
+      pattern: "*"
+
+output:
+  - versions:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+  - reference:
+      type: directory
+      description: directory containing the genome resource files required for fusioncatcher
+      pattern: "fusioncatcher-genome"
+
+authors:
+  - "@praveenraj2018"
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
new file mode 100644
index 00000000..6ed8584f
--- /dev/null
+++ b/modules/local/fusionreport/download/main.nf
@@ -0,0 +1,26 @@
+process FUSIONREPORT_DOWNLOAD {
+    tag 'fusionreport'
+    label 'process_medium'
+
+    // Note: 2.7X indices incompatible with AWS iGenomes.
+    conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'docker.io/rannickscilifelab/fusion-report:2.1.5' :
+        'docker.io/rannickscilifelab/fusion-report:2.1.5' }"
+
+
+    input:
+    val(username)
+    val(passwd)
+
+    output:
+    path "*"                , emit: reference
+    path "versions.yml"     , emit: versions
+
+    script:
+    """
+    fusion_report download --cosmic_usr $username --cosmic_passwd $passwd .
+
+    fusion_report --version > versions.yml
+    """
+}
diff --git a/modules/local/fusionreport/download/meta.yml b/modules/local/fusionreport/download/meta.yml
new file mode 100644
index 00000000..d4bab11a
--- /dev/null
+++ b/modules/local/fusionreport/download/meta.yml
@@ -0,0 +1,35 @@
+name: fusionreport_download
+description: Build DB for fusionreport
+keywords:
+  - sort
+tools:
+  - fusioncatcher:
+      description: Build DB for fusionreport
+      homepage: https://github.com/ndaniel/fusioncatcher/
+      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
+      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
+      doi: "10.1101/011650"
+      licence: ['GPL v3']
+
+input:
+  - username:
+      type: value
+      description: Organism for which the data is downloaded from Ensembl database and built
+      pattern: "*"
+  - passwd:
+      type: value
+      description: Organism for which the data is downloaded from Ensembl database and built
+      pattern: "*"
+
+output:
+  - versions:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+  - reference:
+      type: directory
+      description: directory containing the genome resource files required for fusioncatcher
+      pattern: "fusioncatcher-genome"
+
+authors:
+  - "@praveenraj2018"
diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
new file mode 100644
index 00000000..970763f0
--- /dev/null
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -0,0 +1,44 @@
+//
+// Check input samplesheet and get read channels
+//
+
+include { FUSIONREPORT }                           from '../../modules/local/fusionreport/detect/main'
+
+
+workflow FUSIONREPORT_WORKFLOW {
+    take:
+        reads
+        fusionreport_ref
+        arriba_tools
+
+    main:
+        ch_versions = Channel.empty()
+
+
+    // def tools = !arriba.empty() ? "--arriba ${arriba} " : ''
+    // tools += !ericscript.empty() ? "--ericscript ${ericscript} " : ''
+    // tools += !fusioncatcher.empty() ? "--fusioncatcher ${fusioncatcher} " : ''
+    // tools += !pizzly.empty() ? "--pizzly ${pizzly} " : ''
+    // tools += !squid.empty() ? "--squid ${squid} " : ''
+    // tools += !starfusion.empty() ? "--starfusion ${starfusion} " : ''
+
+        // def tools = !arriba ? "--arriba ${arriba_fusions} " : ''
+
+
+        FUSIONREPORT( reads, fusionreport_ref, arriba_tools )
+        // ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
+        // reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
+
+        // STARFUSION( reads_junction, starfusion_ref)
+        // ch_versions = ch_versions.mix(STARFUSION.out.versions, starfusion_ref )
+
+    emit:
+        versions        = ch_versions.ifEmpty(null) // channel: [ versions.yml ]
+
+    // versions = ch_versions.ifEmpty(null)
+    // STARFUSION.out.fusions
+    // STARFUSION.out.abridged
+    // STARFUSION.out.coding_effect
+
+}
+

From 1db314524dec8a66fb93caef174efa38f42196d1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Feb 2022 11:04:35 +0100
Subject: [PATCH 151/359] temp changes

---
 conf/modules.config    |  5 ++---
 workflows/rnafusion.nf | 42 +++++++++++++++++++++++++++++-------------
 2 files changed, 31 insertions(+), 16 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index ccdc90ab..735370bd 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -70,9 +70,8 @@ process {
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
-        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_2018-11-04.tsv \
-        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_2019-07-05.gff3"
-        // ext.when = params.arriba
+        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv.gz \
+        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
     }
 
     withName: ERICSCRIPT_DOWNLOAD {
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 28c23c98..46b794f8 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -53,6 +53,7 @@ include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_w
 include { PIZZLY_WORKFLOW               }   from '../subworkflows/local/pizzly_workflow'
 include { SQUID_WORKFLOW                }   from '../subworkflows/local/squid_workflow'
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
+include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
 
 /*
 ========================================================================================
@@ -82,6 +83,7 @@ def multiqc_report = []
 workflow RNAFUSION {
 
     ch_versions = Channel.empty()
+    ch_fusions = Channel.empty()
 
     //
     // SUBWORKFLOW: Read in samplesheet, validate and stage input files
@@ -91,6 +93,7 @@ workflow RNAFUSION {
     )
     ch_versions = ch_versions.mix(INPUT_CHECK.out.versions)
 
+
     //
     // MODULE: Run FastQC
     //
@@ -165,13 +168,13 @@ workflow RNAFUSION {
 
 
 
-    // Run ericscript
-    if (params.ericscript){
-        ERICSCRIPT (
-            INPUT_CHECK.out.reads,
-            params.ericscript_ref
-        )
-    }
+    // // Run ericscript
+    // if (params.ericscript){
+    //     ERICSCRIPT (
+    //         INPUT_CHECK.out.reads,
+    //         params.ericscript_ref
+    //     )
+    // }
 
 
     //Run STAR fusion
@@ -189,14 +192,27 @@ workflow RNAFUSION {
 
 
     //Run FusionCatcher
-    if (params.fusioncatcher){
-        FUSIONCATCHER (
-            INPUT_CHECK.out.reads,
-            params.fusioncatcher_ref
-        )
-    }
+    // if (params.fusioncatcher){
+    //     FUSIONCATCHER (
+    //         INPUT_CHECK.out.reads,
+    //         params.fusioncatcher_ref
+    //     )
+    // }
+
+
+
+     //Run fusion-report
+    // FUSIONREPORT_WORKFLOW (
+        // INPUT_CHECK.out.reads,
+        // params.fusionreport_ref,
+        // ARRIBA_WORKFLOW.out.arriba_tools
+    // )
 }
 
+
+
+
+
 /*
 ========================================================================================
     COMPLETION EMAIL AND SUMMARY

From a56b6b08de8f47015622c7ba14f400851f5a22dd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Feb 2022 15:48:11 +0100
Subject: [PATCH 152/359] update arriba module, add rnafusion workflow, NOT IN
 WORKING STATE

---
 conf/modules.config                         |  2 ++
 modules.json                                |  2 +-
 modules/nf-core/modules/arriba/meta.yml     |  2 +-
 subworkflows/local/arriba_workflow.nf       |  7 +++---
 subworkflows/local/fusionreport_workflow.nf |  7 +++---
 workflows/rnafusion.nf                      | 27 ++++++++++-----------
 6 files changed, 23 insertions(+), 24 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 735370bd..8c7cbdb0 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -65,6 +65,7 @@ process {
     }
 
     withName: ARRIBA {
+        ext.when = { params.arriba }
         publishDir = [
             path: { "${params.outdir}/arriba" },
             mode: 'copy',
@@ -146,6 +147,7 @@ process {
 
 //TODO document changes from previous rnafusion version
     withName: STAR_FOR_ARRIBA {
+        ext.when = { params.arriba }
         ext.args = '--readFilesCommand zcat \
         --outSAMtype BAM Unsorted \
         --outSAMunmapped Within \
diff --git a/modules.json b/modules.json
index e11cb973..da3a12a1 100644
--- a/modules.json
+++ b/modules.json
@@ -4,7 +4,7 @@
     "repos": {
         "nf-core/modules": {
             "arriba": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "custom/dumpsoftwareversions": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
diff --git a/modules/nf-core/modules/arriba/meta.yml b/modules/nf-core/modules/arriba/meta.yml
index 6ca16dab..4bde2f08 100644
--- a/modules/nf-core/modules/arriba/meta.yml
+++ b/modules/nf-core/modules/arriba/meta.yml
@@ -10,7 +10,7 @@ tools:
       documentation: https://arriba.readthedocs.io/en/latest/
       tool_dev_url: https://github.com/suhrig/arriba
       doi: "10.1101/gr.257246.119"
-      licence: ['MIT']
+      licence: ["MIT"]
 
 input:
   - meta:
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 119c33fd..a24e85c9 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -23,15 +23,14 @@ workflow ARRIBA_WORKFLOW {
         STAR_FOR_ARRIBA( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
         ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
 
-        // reads_align = reads.join(STAR_FOR_STARFUSION.out.junction )
 
         ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
         ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
 
     emit:
-        ARRIBA.out.fusions
-        ARRIBA.out.fusions_fail
-        versions = ch_versions.ifEmpty(null)
+        fusions         = ARRIBA.out.fusions
+        fusions_fail    = ARRIBA.out.fusions_fail
+        versions        = ch_versions.ifEmpty(null)
     }
 
diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index 970763f0..664c6446 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -9,13 +9,13 @@ workflow FUSIONREPORT_WORKFLOW {
     take:
         reads
         fusionreport_ref
-        arriba_tools
+        fusions
 
     main:
         ch_versions = Channel.empty()
 
 
-    // def tools = !arriba.empty() ? "--arriba ${arriba} " : ''
+    tools = {fusions == [] ? "--arriba ${fusions} " : ''}
     // tools += !ericscript.empty() ? "--ericscript ${ericscript} " : ''
     // tools += !fusioncatcher.empty() ? "--fusioncatcher ${fusioncatcher} " : ''
     // tools += !pizzly.empty() ? "--pizzly ${pizzly} " : ''
@@ -24,8 +24,7 @@ workflow FUSIONREPORT_WORKFLOW {
 
         // def tools = !arriba ? "--arriba ${arriba_fusions} " : ''
 
-
-        FUSIONREPORT( reads, fusionreport_ref, arriba_tools )
+        FUSIONREPORT( reads, fusionreport_ref, tools )
         // ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
         // reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 46b794f8..e15b3af5 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -129,16 +129,15 @@ workflow RNAFUSION {
 
 
     // Run STAR alignment and Arriba
-    if (params.arriba){
-        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+    gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+
+    ARRIBA_WORKFLOW (
+        INPUT_CHECK.out.reads,
+        params.fasta,
+        params.starindex_ref,
+        gtf
+    )
 
-        ARRIBA_WORKFLOW (
-            INPUT_CHECK.out.reads,
-            params.fasta,
-            params.starindex_ref,
-            gtf
-        )
-    }
 
     // Run pizzly/kallisto
     if (params.pizzly){
@@ -202,11 +201,11 @@ workflow RNAFUSION {
 
 
      //Run fusion-report
-    // FUSIONREPORT_WORKFLOW (
-        // INPUT_CHECK.out.reads,
-        // params.fusionreport_ref,
-        // ARRIBA_WORKFLOW.out.arriba_tools
-    // )
+    FUSIONREPORT_WORKFLOW (
+        INPUT_CHECK.out.reads,
+        params.fusionreport_ref,
+        ARRIBA_WORKFLOW.out.fusions
+    )
 }
 
 

From 17acd8598ef7ca05038003683be57e95b3ddbf6c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:06:43 +0100
Subject: [PATCH 153/359] remove when as logic handled in workflow, clarify
 publishing directory for the different star instances

---
 conf/modules.config | 35 ++++++++++++++++++++++++++++++-----
 1 file changed, 30 insertions(+), 5 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 8c7cbdb0..4c874b9f 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -65,7 +65,6 @@ process {
     }
 
     withName: ARRIBA {
-        ext.when = { params.arriba }
         publishDir = [
             path: { "${params.outdir}/arriba" },
             mode: 'copy',
@@ -91,9 +90,9 @@ process {
     ]
     }
 
-    withName: FUSIONCATCHER_DOWNLOAD {
-        ext.args = "--continue --tries=42"
-    }
+    // withName: FUSIONCATCHER_DOWNLOAD {
+    //     ext.args = "--continue --tries=42"
+    // }
 
     // withName: FUSIONCATCHER {
         // ext.args = "--continue --tries=42"
@@ -121,6 +120,11 @@ process {
 
 //TODO document changes from previous rnafusion version to use star-fusion recommendations
     withName: STAR_FOR_STARFUSION {
+        publishDir = [
+            path: { "${params.outdir}/star_for_starfusion" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
         ext.args = '--twopassMode Basic \
         --outReadsUnmapped None \
         --readFilesCommand zcat \
@@ -147,7 +151,11 @@ process {
 
 //TODO document changes from previous rnafusion version
     withName: STAR_FOR_ARRIBA {
-        ext.when = { params.arriba }
+        publishDir = [
+            path: { "${params.outdir}/star_for_arriba" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
         ext.args = '--readFilesCommand zcat \
         --outSAMtype BAM Unsorted \
         --outSAMunmapped Within \
@@ -167,6 +175,11 @@ process {
     }
 
     withName: STAR_FOR_SQUID {
+        publishDir = [
+            path: { "${params.outdir}/star_for_squid" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
         ext.args = '--twopassMode Basic \
         --chimOutType SeparateSAMold \
         --chimSegmentMin 20 \
@@ -180,6 +193,11 @@ process {
 
     withName: PIZZLY {
         ext.args = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
+        publishDir = [
+            path: { "${params.outdir}/pizzly" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
     }
 
     withName: SAMTOOLS_VIEW {
@@ -190,6 +208,13 @@ process {
         ext.prefix = { "${meta.id}_chimeric_sorted" }
     }
 
+    withName: GET_PATH {
+        publishDir = [
+            path: { "${params.outdir}/collected_fusions" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
+    }
 }
 
 

From 6b5e219a26964384a2aeb09f4d4d1607a902e224 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:21:52 +0100
Subject: [PATCH 154/359] add fusionreport

---
 modules/local/fusionreport/detect/main.nf | 21 +++++++++++++++------
 1 file changed, 15 insertions(+), 6 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 44a4c824..c6397929 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -12,17 +12,26 @@ process FUSIONREPORT {
     input:
     tuple val(meta), path(reads)
     path(fusionreport_ref)
-    file(arriba_tools)
+    path(arriba_fusions)
+    path(pizzly_fusions)
+    path(squid_fusions)
+    path(starfusion_fusions)
 
     output:
-    path "*"                , emit: reference
-    path "versions.yml"     , emit: versions
+    path "versions.yml"           , emit: versions
+    tuple val(meta), path("*.tsv") , emit: fusion_list
 
     script:
-
+    def tools = params.arriba      ? "--arriba ${arriba_fusions} " : ''
+    tools    += params.pizzly      ? "--pizzly ${pizzly_fusions} " : ''
+    tools    += params.squid       ? "--squid ${squid_fusions} " : ''
+    tools    += params.starfusion  ? "--squid ${starfusion_fusions} " : ''
     """
-    fusion_report run $meta.id . $fusionreport_ref --arriba $arriba_tools --allow-multiple-gene-symbols
+    fusion_report run $meta.id . $fusionreport_ref $tools --allow-multiple-gene-symbols
 
-    fusion_report --version > versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusion_report: \$(fusion_report --version)
+    END_VERSIONS
     """
 }

From b707b97fec30b3de2c32c3fb546873330d86b023 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:22:10 +0100
Subject: [PATCH 155/359] add fusionreport meta

---
 modules/local/fusionreport/detect/meta.yml | 31 +++++++++++-----------
 1 file changed, 16 insertions(+), 15 deletions(-)

diff --git a/modules/local/fusionreport/detect/meta.yml b/modules/local/fusionreport/detect/meta.yml
index d4bab11a..dd3faf11 100644
--- a/modules/local/fusionreport/detect/meta.yml
+++ b/modules/local/fusionreport/detect/meta.yml
@@ -1,10 +1,10 @@
-name: fusionreport_download
-description: Build DB for fusionreport
+name: fusionreport
+description: fusionreport
 keywords:
   - sort
 tools:
-  - fusioncatcher:
-      description: Build DB for fusionreport
+  - fusionreport:
+      description: fusionreport
       homepage: https://github.com/ndaniel/fusioncatcher/
       documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
       tool_dev_url: https://github.com/ndaniel/fusioncatcher/
@@ -12,24 +12,25 @@ tools:
       licence: ['GPL v3']
 
 input:
-  - username:
-      type: value
-      description: Organism for which the data is downloaded from Ensembl database and built
-      pattern: "*"
-  - passwd:
-      type: value
-      description: Organism for which the data is downloaded from Ensembl database and built
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reference:
+      type: path
+      description: DB
       pattern: "*"
+  - fusions:
+      type: path
+      description: File contains fusions which pass all of Arriba's filters.
+      pattern: "*.fusions.tsv"
 
 output:
   - versions:
         type: file
         description: File containing software versions
         pattern: "versions.yml"
-  - reference:
-      type: directory
-      description: directory containing the genome resource files required for fusioncatcher
-      pattern: "fusioncatcher-genome"
 
 authors:
   - "@praveenraj2018"

From 954fa835d4ccc319dbabb51fd0a1d86b9cb0cf05 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:24:13 +0100
Subject: [PATCH 156/359] add dummy files to feed in fusion_report if a tool in
 not used

---
 assets/dummy_file_arriba.txt     | 0
 assets/dummy_file_pizzly.txt     | 0
 assets/dummy_file_squid.txt      | 0
 assets/dummy_file_starfusion.txt | 0
 4 files changed, 0 insertions(+), 0 deletions(-)
 create mode 100644 assets/dummy_file_arriba.txt
 create mode 100644 assets/dummy_file_pizzly.txt
 create mode 100644 assets/dummy_file_squid.txt
 create mode 100644 assets/dummy_file_starfusion.txt

diff --git a/assets/dummy_file_arriba.txt b/assets/dummy_file_arriba.txt
new file mode 100644
index 00000000..e69de29b
diff --git a/assets/dummy_file_pizzly.txt b/assets/dummy_file_pizzly.txt
new file mode 100644
index 00000000..e69de29b
diff --git a/assets/dummy_file_squid.txt b/assets/dummy_file_squid.txt
new file mode 100644
index 00000000..e69de29b
diff --git a/assets/dummy_file_starfusion.txt b/assets/dummy_file_starfusion.txt
new file mode 100644
index 00000000..e69de29b

From ef2f59ea1becdd7e2447176b3ebb7a5b9e138d88 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:25:28 +0100
Subject: [PATCH 157/359] add process GET_PATH that takes in a tuple meta/path
 and returns a path only; for compatibility between the different
 fusion_report feeding option (file, dummy file, pipeline output with tuple)

---
 modules/local/getpath/main.nf | 14 ++++++++++++++
 1 file changed, 14 insertions(+)
 create mode 100644 modules/local/getpath/main.nf

diff --git a/modules/local/getpath/main.nf b/modules/local/getpath/main.nf
new file mode 100644
index 00000000..7bc72c0d
--- /dev/null
+++ b/modules/local/getpath/main.nf
@@ -0,0 +1,14 @@
+process GET_PATH {
+    tag 'getpath'
+    label 'process_low'
+
+    input:
+    tuple val(meta), path(file)
+
+    output:
+    path file                , emit: file
+
+    script:
+    """
+    """
+}

From 529112caa9202461052d2a54ed5e961ed73a78ac Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:27:45 +0100
Subject: [PATCH 158/359] clean-up rnafusion workflow as all logic will be
 handled in subworkflows

---
 workflows/rnafusion.nf | 68 ++++++++++--------------------------------
 1 file changed, 16 insertions(+), 52 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index e15b3af5..660e134a 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -83,7 +83,6 @@ def multiqc_report = []
 workflow RNAFUSION {
 
     ch_versions = Channel.empty()
-    ch_fusions = Channel.empty()
 
     //
     // SUBWORKFLOW: Read in samplesheet, validate and stage input files
@@ -97,7 +96,6 @@ workflow RNAFUSION {
     //
     // MODULE: Run FastQC
     //
-
     FASTQC (
         INPUT_CHECK.out.reads
     )
@@ -127,68 +125,32 @@ workflow RNAFUSION {
     multiqc_report       = MULTIQC.out.report.toList()
     ch_versions          = ch_versions.mix(MULTIQC.out.versions)
 
-
     // Run STAR alignment and Arriba
-    gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-
     ARRIBA_WORKFLOW (
         INPUT_CHECK.out.reads,
         params.fasta,
         params.starindex_ref,
-        gtf
     )
 
-
     // Run pizzly/kallisto
-    if (params.pizzly){
-        index ="${params.pizzly_ref}/kallisto"
-        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-        transcript ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
-
-        PIZZLY_WORKFLOW (
-            INPUT_CHECK.out.reads,
-            index,
-            gtf,
-            transcript
-        )
-    }
-
-    // Run squid
-    if (params.squid){
-        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-
-        SQUID_WORKFLOW (
-            INPUT_CHECK.out.reads,
-            params.fasta,
-            params.starindex_ref,
-            gtf
-        )
-    }
-
 
+    PIZZLY_WORKFLOW (
+        INPUT_CHECK.out.reads,
+    )
 
-    // // Run ericscript
-    // if (params.ericscript){
-    //     ERICSCRIPT (
-    //         INPUT_CHECK.out.reads,
-    //         params.ericscript_ref
-    //     )
-    // }
 
+// Run squid
+    SQUID_WORKFLOW (
+        INPUT_CHECK.out.reads,
+        params.fasta
+    )
 
-    //Run STAR fusion
-    if (params.starfusion){
-        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
-        index ="${params.starfusion_ref}/ctat_genome_lib_build_dir"
 
-        STARFUSION_WORKFLOW (
-            INPUT_CHECK.out.reads,
-            params.starindex_ref,
-            gtf,
-            index
-        )
-    }
+//Run STAR fusion
 
+    STARFUSION_WORKFLOW (
+        INPUT_CHECK.out.reads
+    )
 
     //Run FusionCatcher
     // if (params.fusioncatcher){
@@ -204,14 +166,16 @@ workflow RNAFUSION {
     FUSIONREPORT_WORKFLOW (
         INPUT_CHECK.out.reads,
         params.fusionreport_ref,
-        ARRIBA_WORKFLOW.out.fusions
+        ARRIBA_WORKFLOW.out.fusions,
+        PIZZLY_WORKFLOW.out.fusions,
+        SQUID_WORKFLOW.out.fusions,
+        STARFUSION_WORKFLOW.out.fusions
     )
 }
 
 
 
 
-
 /*
 ========================================================================================
     COMPLETION EMAIL AND SUMMARY

From fe38ae208e77bd24d58f3548739ae0e81b78ad7c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:30:05 +0100
Subject: [PATCH 159/359] arriba: logic handles in subworkflow, definition of
 gtf in subworkflow, dummy file used in case tool in not used to handle
 dependencies properly

---
 subworkflows/local/arriba_workflow.nf | 31 +++++++++++++++++++--------
 1 file changed, 22 insertions(+), 9 deletions(-)

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index a24e85c9..76db20d5 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -4,6 +4,7 @@
 
 include { STAR_ALIGN as STAR_FOR_ARRIBA }    from '../../modules/nf-core/modules/star/align/main'
 include { ARRIBA }                           from '../../modules/nf-core/modules/arriba/main'
+include { GET_PATH }                         from '../../modules/local/getpath/main'
 
 
 workflow ARRIBA_WORKFLOW {
@@ -11,25 +12,37 @@ workflow ARRIBA_WORKFLOW {
         reads
         fasta
         index
-        gtf
 
     main:
         ch_versions = Channel.empty()
+        ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
-        star_ignore_sjdbgtf = false
-        seq_platform = false
-        seq_center = false
+        if (params.arriba) {
+            if (params.arriba_fusions) {
+                ch_arriba_fusions = params.arriba_fusions
+            } else {
+                gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+                star_ignore_sjdbgtf = false
+                seq_platform = false
+                seq_center = false
 
-        STAR_FOR_ARRIBA( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
-        ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
+                STAR_FOR_ARRIBA( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+                ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
 
 
-        ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
-        ch_versions = ch_versions.mix(ARRIBA.out.versions)
+                ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
+                ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
+                GET_PATH(ARRIBA.out.fusions)
+                ch_arriba_fusions = GET_PATH.out.file
+            }
+        }
+        else {
+            ch_arriba_fusions = ch_dummy_file
+        }
 
     emit:
-        fusions         = ARRIBA.out.fusions
+        fusions         = ch_arriba_fusions
         fusions_fail    = ARRIBA.out.fusions_fail
         versions        = ch_versions.ifEmpty(null)
     }

From f1fec567ad796be7811d7e4d2c41b487c83e15f1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:31:00 +0100
Subject: [PATCH 160/359] pizzly: logic handles in subworkflow, definition of
 gtf in subworkflow, dummy file used in case tool in not used to handle
 dependencies properly

---
 subworkflows/local/pizzly_workflow.nf | 32 ++++++++++++++++++---------
 1 file changed, 22 insertions(+), 10 deletions(-)

diff --git a/subworkflows/local/pizzly_workflow.nf b/subworkflows/local/pizzly_workflow.nf
index 98286288..52bdd44b 100644
--- a/subworkflows/local/pizzly_workflow.nf
+++ b/subworkflows/local/pizzly_workflow.nf
@@ -4,30 +4,42 @@
 
 include { KALLISTO_QUANT    }     from '../../modules/local/kallisto/quant/main'
 include { PIZZLY            }     from '../../modules/local/pizzly/detect/main'
+include { GET_PATH          }     from '../../modules/local/getpath/main'
 
 
 workflow PIZZLY_WORKFLOW {
     take:
         reads
-        index
-        gtf
-        transcript
 
     main:
         ch_versions = Channel.empty()
+        ch_dummy_file = file("$baseDir/assets/dummy_file_pizzly.txt", checkIfExists: true)
 
-        KALLISTO_QUANT( reads, index )
-        ch_versions = ch_versions.mix(KALLISTO_QUANT.out.versions)
+        if (params.pizzly) {
+            if (params.pizzly_fusions) {
+                ch_pizzly_fusions = params.pizzly_fusions
+            } else {
+                index ="${params.pizzly_ref}/kallisto"
+                gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+                transcript ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
 
+                KALLISTO_QUANT( reads, index )
+                ch_versions = ch_versions.mix(KALLISTO_QUANT.out.versions)
 
+                PIZZLY( KALLISTO_QUANT.out.txt, transcript, gtf )
+                ch_versions = ch_versions.mix(PIZZLY.out.versions)
 
-        PIZZLY( KALLISTO_QUANT.out.txt, transcript, gtf )
-        // ch_versions = ch_versions.mix(ARRIBA.out.versions)
+                GET_PATH(PIZZLY.out.fusions)
+                ch_pizzly_fusions = GET_PATH.out.file
+            }
+        }
+        else  {
+            ch_pizzly_fusions = ch_dummy_file
 
+        }
 
     emit:
-        KALLISTO_QUANT.out.txt
-        // ARRIBA.out.fusions_fail
-        // versions = ch_versions.ifEmpty(null)
+        fusions             = ch_pizzly_fusions
+        versions            = ch_versions.ifEmpty(null)
     }
 

From 3320afe9593422e3842127c70f3fc419dd12e9e3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:32:11 +0100
Subject: [PATCH 161/359] squid: logic handles in subworkflow, definition of
 gtf in subworkflow, dummy file used in case tool in not used to handle
 dependencies properly

---
 subworkflows/local/squid_workflow.nf | 50 +++++++++++++++++-----------
 1 file changed, 31 insertions(+), 19 deletions(-)

diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 98dada05..6e3aee8f 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -7,43 +7,55 @@ include { SAMTOOLS_VIEW }                   from '../../modules/nf-core/modules/
 include { SAMTOOLS_SORT }                   from '../../modules/nf-core/modules/samtools/sort/main'
 include { SQUID }                           from '../../modules/local/squid/detect/main'
 include { SQUID_ANNOTATE }                  from '../../modules/local/squid/annotate/main'
+include { GET_PATH }                         from '../../modules/local/getpath/main'
 
 
 workflow SQUID_WORKFLOW {
     take:
         reads
-        fasta
-        index
-        gtf
+        fast
 
     main:
         ch_versions = Channel.empty()
+        ch_dummy_file = file("$baseDir/assets/dummy_file_squid.txt", checkIfExists: true)
 
-        star_ignore_sjdbgtf = false
-        seq_platform = false
-        seq_center = false
+        if (params.squid) {
+            if (params.squid_fusions){
+                ch_squid_fusions = params.squid_fusions
+            } else {
+            gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+            star_ignore_sjdbgtf = false
+            seq_platform = false
+            seq_center = false
+            index = params.starindex_ref
 
-        STAR_FOR_SQUID( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
-        ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
+            STAR_FOR_SQUID( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+            ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
-        SAMTOOLS_VIEW ( STAR_FOR_SQUID.out.sam, [] )
-        ch_versions = ch_versions.mix(SAMTOOLS_VIEW.out.versions )
+            SAMTOOLS_VIEW ( STAR_FOR_SQUID.out.sam, [] )
+            ch_versions = ch_versions.mix(SAMTOOLS_VIEW.out.versions )
 
-        SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam )
-        ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions )
+            SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam )
+            ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions )
 
-        bam_sorted = STAR_FOR_SQUID.out.bam_sorted.join(SAMTOOLS_SORT.out.bam )
+            bam_sorted = STAR_FOR_SQUID.out.bam_sorted.join(SAMTOOLS_SORT.out.bam )
 
-        SQUID ( bam_sorted )
-        ch_versions = ch_versions.mix(SQUID.out.versions)
+            SQUID ( bam_sorted )
+            ch_versions = ch_versions.mix(SQUID.out.versions)
 
-        SQUID_ANNOTATE ( SQUID.out.fusions, gtf )
-        ch_versions = ch_versions.mix(SQUID_ANNOTATE.out.versions)
+            SQUID_ANNOTATE ( SQUID.out.fusions, gtf )
+            ch_versions = ch_versions.mix(SQUID_ANNOTATE.out.versions)
 
+            GET_PATH(SQUID_ANNOTATE.out.fusions_annotated)
+            ch_squid_fusions = GET_PATH.out.file
+            }
+        }
+        else {
+            ch_squid_fusions = ch_dummy_file
+        }
 
     emit:
-        // ARRIBA.out.fusions
-        // ARRIBA.out.fusions_fail
+        fusions  = ch_squid_fusions
         versions = ch_versions.ifEmpty(null)
     }
 

From c8d100ff81bae503f74ef2c65c6d50f31aa987e5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:42:24 +0100
Subject: [PATCH 162/359] starfusion: logic handles in subworkflow, definition
 of gtf in subworkflow, dummy file used in case tool in not used to handle
 dependencies properly

---
 subworkflows/local/starfusion_workflow.nf | 49 ++++++++++++++---------
 1 file changed, 29 insertions(+), 20 deletions(-)

diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index ed5eaac2..4613ffe7 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -9,31 +9,40 @@ include { STARFUSION }                           from '../../modules/local/starf
 workflow STARFUSION_WORKFLOW {
     take:
         reads
-        index
-        gtf
-        starfusion_ref
 
     main:
         ch_versions = Channel.empty()
-
-        star_ignore_sjdbgtf = false
-        seq_platform = false
-        seq_center = false
-
-        STAR_FOR_STARFUSION( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
-        // ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
-        reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
-
-        STARFUSION( reads_junction, starfusion_ref)
-        // ch_versions = ch_versions.mix(STARFUSION.out.versions, starfusion_ref )
-
+        ch_dummy_file = file("$baseDir/assets/dummy_file_starfusion.txt", checkIfExists: true)
+
+        if (params.starfusion){
+            if (params.starfusion_fusions){
+                ch_starfusion_fusions = params.starfusion_fusions
+            } else {
+                gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
+                index ="${params.starfusion_ref}/ctat_genome_lib_build_dir"
+
+                star_ignore_sjdbgtf = false
+                seq_platform = false
+                seq_center = false
+
+                STAR_FOR_STARFUSION( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+                ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
+                reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
+
+                STARFUSION( reads_junction, starfusion_ref)
+                ch_versions = ch_versions.mix(STARFUSION.out.versions, params.starfusion_ref )
+
+                GET_PATH(STARFUSION.out.fusions)
+                ch_starfusion_fusions = GET_PATH.out.file
+            }
+        }
+        else {
+            ch_starfusion_fusions = ch_dummy_file
+        }
     emit:
-        versions        = ch_versions.ifEmpty(null) // channel: [ versions.yml ]
+        fusions         = ch_starfusion_fusions
+        versions        = ch_versions.ifEmpty(null)
 
-    // versions = ch_versions.ifEmpty(null)
-    // STARFUSION.out.fusions
-    // STARFUSION.out.abridged
-    // STARFUSION.out.coding_effect
 
 }
 

From c46a7dc3d22d1155d26e7b336e56789305d0da92 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 14:43:19 +0100
Subject: [PATCH 163/359] cleanup fusionreport workflow

---
 subworkflows/local/fusionreport_workflow.nf | 32 ++++++---------------
 1 file changed, 8 insertions(+), 24 deletions(-)

diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index 664c6446..3083ff38 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -9,35 +9,19 @@ workflow FUSIONREPORT_WORKFLOW {
     take:
         reads
         fusionreport_ref
-        fusions
-
+        arriba_fusions
+        pizzly_fusions
+        squid_fusions
+        starfusion_fusions
     main:
         ch_versions = Channel.empty()
 
-
-    tools = {fusions == [] ? "--arriba ${fusions} " : ''}
-    // tools += !ericscript.empty() ? "--ericscript ${ericscript} " : ''
-    // tools += !fusioncatcher.empty() ? "--fusioncatcher ${fusioncatcher} " : ''
-    // tools += !pizzly.empty() ? "--pizzly ${pizzly} " : ''
-    // tools += !squid.empty() ? "--squid ${squid} " : ''
-    // tools += !starfusion.empty() ? "--starfusion ${starfusion} " : ''
-
-        // def tools = !arriba ? "--arriba ${arriba_fusions} " : ''
-
-        FUSIONREPORT( reads, fusionreport_ref, tools )
-        // ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
-        // reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
-
-        // STARFUSION( reads_junction, starfusion_ref)
-        // ch_versions = ch_versions.mix(STARFUSION.out.versions, starfusion_ref )
+        FUSIONREPORT( reads, fusionreport_ref, arriba_fusions, pizzly_fusions, squid_fusions, starfusion_fusions )
+        ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
 
     emit:
-        versions        = ch_versions.ifEmpty(null) // channel: [ versions.yml ]
-
-    // versions = ch_versions.ifEmpty(null)
-    // STARFUSION.out.fusions
-    // STARFUSION.out.abridged
-    // STARFUSION.out.coding_effect
+        versions        = ch_versions.ifEmpty(null)
+        fusion_list     = FUSIONREPORT.out.fusion_list
 
 }
 

From d9272ff9de87d307a04d5d77bbd18270db36655f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 15:11:37 +0100
Subject: [PATCH 164/359] add cat/fastq module

---
 modules.json                               |  3 ++
 modules/nf-core/modules/cat/fastq/main.nf  | 51 ++++++++++++++++++++++
 modules/nf-core/modules/cat/fastq/meta.yml | 39 +++++++++++++++++
 3 files changed, 93 insertions(+)
 create mode 100644 modules/nf-core/modules/cat/fastq/main.nf
 create mode 100644 modules/nf-core/modules/cat/fastq/meta.yml

diff --git a/modules.json b/modules.json
index da3a12a1..afd3b759 100644
--- a/modules.json
+++ b/modules.json
@@ -6,6 +6,9 @@
             "arriba": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
+            "cat/fastq": {
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+            },
             "custom/dumpsoftwareversions": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
diff --git a/modules/nf-core/modules/cat/fastq/main.nf b/modules/nf-core/modules/cat/fastq/main.nf
new file mode 100644
index 00000000..bf0877c3
--- /dev/null
+++ b/modules/nf-core/modules/cat/fastq/main.nf
@@ -0,0 +1,51 @@
+process CAT_FASTQ {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
+        'biocontainers/biocontainers:v1.2.0_cv1' }"
+
+    input:
+    tuple val(meta), path(reads, stageAs: "input*/*")
+
+    output:
+    tuple val(meta), path("*.merged.fastq.gz"), emit: reads
+    path "versions.yml"                       , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def readList = reads.collect{ it.toString() }
+    if (meta.single_end) {
+        if (readList.size > 1) {
+            """
+            cat ${readList.join(' ')} > ${prefix}.merged.fastq.gz
+
+            cat <<-END_VERSIONS > versions.yml
+            "${task.process}":
+                cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
+            END_VERSIONS
+            """
+        }
+    } else {
+        if (readList.size > 2) {
+            def read1 = []
+            def read2 = []
+            readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v }
+            """
+            cat ${read1.join(' ')} > ${prefix}_1.merged.fastq.gz
+            cat ${read2.join(' ')} > ${prefix}_2.merged.fastq.gz
+
+            cat <<-END_VERSIONS > versions.yml
+            "${task.process}":
+                cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
+            END_VERSIONS
+            """
+        }
+    }
+}
diff --git a/modules/nf-core/modules/cat/fastq/meta.yml b/modules/nf-core/modules/cat/fastq/meta.yml
new file mode 100644
index 00000000..c836598e
--- /dev/null
+++ b/modules/nf-core/modules/cat/fastq/meta.yml
@@ -0,0 +1,39 @@
+name: cat_fastq
+description: Concatenates fastq files
+keywords:
+  - fastq
+  - concatenate
+tools:
+  - cat:
+      description: |
+        The cat utility reads files sequentially, writing them to the standard output.
+      documentation: https://www.gnu.org/software/coreutils/manual/html_node/cat-invocation.html
+      licence: ["GPL-3.0-or-later"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: list
+      description: |
+        List of input FastQ files to be concatenated.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: Merged fastq file
+      pattern: "*.{merged.fastq.gz}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@joseespinosa"
+  - "@drpatelh"

From 9420e9821ce3102ecf4d7525fac5f7865315a488 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 15:42:01 +0100
Subject: [PATCH 165/359] integrate cat/fastq in rnafusion workflow

---
 workflows/rnafusion.nf | 42 ++++++++++++++++++++++++++++++++++++------
 1 file changed, 36 insertions(+), 6 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 660e134a..bceac718 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -45,6 +45,7 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 //
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
+include { CAT_FASTQ                     }   from '../modules/nf-core/modules/cat/fastq/main'
 include { INPUT_CHECK                   }   from '../subworkflows/local/input_check'
 include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
 include { FUSIONCATCHER                 }   from '../modules/local/fusioncatcher/detect/main'
@@ -90,14 +91,36 @@ workflow RNAFUSION {
     INPUT_CHECK (
         ch_input
     )
+    .reads
+    .map {
+        meta, fastq ->
+            meta.id = meta.id.split('_')[0..-2].join('_')
+            [ meta, fastq ] }
+    .groupTuple(by: [0])
+    .branch {
+        meta, fastq ->
+            single  : fastq.size() == 1
+                return [ meta, fastq.flatten() ]
+            multiple: fastq.size() > 1
+                return [ meta, fastq.flatten() ]
+    }
+    .set { ch_fastq }
     ch_versions = ch_versions.mix(INPUT_CHECK.out.versions)
 
+    CAT_FASTQ (
+        ch_fastq.multiple
+    )
+    .reads
+    .mix(ch_fastq.single)
+    .set { ch_cat_fastq }
+    ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first().ifEmpty(null))
+
 
     //
     // MODULE: Run FastQC
     //
     FASTQC (
-        INPUT_CHECK.out.reads
+        ch_cat_fastq
     )
     ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
@@ -125,32 +148,39 @@ workflow RNAFUSION {
     multiqc_report       = MULTIQC.out.report.toList()
     ch_versions          = ch_versions.mix(MULTIQC.out.versions)
 
+
+
     // Run STAR alignment and Arriba
     ARRIBA_WORKFLOW (
-        INPUT_CHECK.out.reads,
+        ch_cat_fastq,
         params.fasta,
         params.starindex_ref,
     )
+    ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions.first().ifEmpty(null))
 
     // Run pizzly/kallisto
 
     PIZZLY_WORKFLOW (
-        INPUT_CHECK.out.reads,
+        ch_cat_fastq,
     )
+    ch_versions = ch_versions.mix(PIZZLY_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
 // Run squid
     SQUID_WORKFLOW (
-        INPUT_CHECK.out.reads,
+        ch_cat_fastq,
         params.fasta
     )
+    ch_versions = ch_versions.mix(SQUID_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
 //Run STAR fusion
 
     STARFUSION_WORKFLOW (
-        INPUT_CHECK.out.reads
+        ch_cat_fastq
     )
+    ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions.first().ifEmpty(null))
+
 
     //Run FusionCatcher
     // if (params.fusioncatcher){
@@ -164,7 +194,7 @@ workflow RNAFUSION {
 
      //Run fusion-report
     FUSIONREPORT_WORKFLOW (
-        INPUT_CHECK.out.reads,
+        ch_cat_fastq,
         params.fusionreport_ref,
         ARRIBA_WORKFLOW.out.fusions,
         PIZZLY_WORKFLOW.out.fusions,

From 4a52bd190fe614e1ecafcffb1c117cfa77c86994 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 15:54:11 +0100
Subject: [PATCH 166/359] linting attemps

---
 modules/local/starfusion/download/main.nf | 1 -
 1 file changed, 1 deletion(-)

diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index db20d588..13955427 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -41,5 +41,4 @@ process STARFUSION_DOWNLOAD {
         --max_readlength $params.read_length \\
         --CPU $task.cpus
     """
-
 }

From e187becf50651dc28e0cd2f09593334fe9fefc5a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 16:04:01 +0100
Subject: [PATCH 167/359] linting attemps

---
 workflows/rnafusion.nf | 29 -----------------------------
 1 file changed, 29 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2ff43b01..8895c22e 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -44,13 +44,10 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
 include { INPUT_CHECK                   } from '../subworkflows/local/input_check'
-
 include { FUSIONCATCHER                 }   from '../modules/local/fusioncatcher/detect/main'
-// include { ERICSCRIPT                    }   from '../modules/local/ericscript/detect/main'
 
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
-// include { PIZZLY_WORKFLOW               }   from '../subworkflows/local/pizzly_workflow'
 
 /*
 ========================================================================================
@@ -135,25 +132,6 @@ workflow RNAFUSION {
         )
     }
 
-    // // Run pizzly/kallisto
-    // if (params.pizzly){
-    //     index ="${params.pizzly_ref}/kallisto.idx"
-    //     PIZZLY_WORKFLOW (
-    //         INPUT_CHECK.out.reads,
-    //         index
-    //     )
-    // }
-
-
-    // // Run ericscript
-    // if (params.ericscript){
-    //     ERICSCRIPT (
-    //         INPUT_CHECK.out.reads,
-    //         params.ericscript_ref
-    //     )
-    // }
-
-
     //Run STAR fusion
     if (params.starfusion){
         gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
@@ -166,13 +144,6 @@ workflow RNAFUSION {
             index
         )
     }
-    // //Run FusionCatcher
-    // if (params.fusioncatcher){
-    //     FUSIONCATCHER (
-    //         INPUT_CHECK.out.reads,
-    //         params.fusioncatcher_ref
-    //     )
-    // }
 }
 
 /*

From 1896a0676d561b1b87bd5e78f50a725bc21e3357 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 16:05:08 +0100
Subject: [PATCH 168/359] update arriba nf-core module

---
 modules.json                            | 2 +-
 modules/nf-core/modules/arriba/meta.yml | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules.json b/modules.json
index 8c0ca267..5851a32f 100644
--- a/modules.json
+++ b/modules.json
@@ -4,7 +4,7 @@
     "repos": {
         "nf-core/modules": {
             "arriba": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "custom/dumpsoftwareversions": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
diff --git a/modules/nf-core/modules/arriba/meta.yml b/modules/nf-core/modules/arriba/meta.yml
index 6ca16dab..4bde2f08 100644
--- a/modules/nf-core/modules/arriba/meta.yml
+++ b/modules/nf-core/modules/arriba/meta.yml
@@ -10,7 +10,7 @@ tools:
       documentation: https://arriba.readthedocs.io/en/latest/
       tool_dev_url: https://github.com/suhrig/arriba
       doi: "10.1101/gr.257246.119"
-      licence: ['MIT']
+      licence: ["MIT"]
 
 input:
   - meta:

From 11f284e5aed19245f8161e2cc09f2be980d2c587 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Feb 2022 18:01:47 +0100
Subject: [PATCH 169/359] add fusioncatcher

---
 assets/dummy_file_fusioncatcher.txt          |   0
 build-ctat.nf                                | 162 -------------------
 conf/modules.config                          |  14 +-
 containers/fusioncatcher/Dockerfile          |  55 +++++--
 containers/fusioncatcher/environment.yml     |   7 -
 modules/local/fusioncatcher/detect/main.nf   |  27 ++--
 modules/local/fusioncatcher/download/main.nf |  11 +-
 nextflow.config                              |   7 +-
 nextflow_schema.json                         |  26 +++
 subworkflows/local/fusioncatcher_workflow.nf |  38 +++++
 workflows/rnafusion.nf                       |  15 +-
 11 files changed, 150 insertions(+), 212 deletions(-)
 create mode 100644 assets/dummy_file_fusioncatcher.txt
 delete mode 100644 build-ctat.nf
 delete mode 100644 containers/fusioncatcher/environment.yml
 create mode 100644 subworkflows/local/fusioncatcher_workflow.nf

diff --git a/assets/dummy_file_fusioncatcher.txt b/assets/dummy_file_fusioncatcher.txt
new file mode 100644
index 00000000..e69de29b
diff --git a/build-ctat.nf b/build-ctat.nf
deleted file mode 100644
index 8df8d500..00000000
--- a/build-ctat.nf
+++ /dev/null
@@ -1,162 +0,0 @@
-#!/usr/bin/env nextflow
-/*
-================================================================================
-                                nf-core/rnafusion
-================================================================================
-nf-core/rnafusion:
-RNA-seq analysis pipeline for detection gene-fusions
---------------------------------------------------------------------------------
-@Homepage
-https://nf-co.re/rnafusion
---------------------------------------------------------------------------------
-@Documentation
-https://nf-co.re/rnafusion/docs
---------------------------------------------------------------------------------
-@Repository
-https://github.com/nf-core/rnafusion
---------------------------------------------------------------------------------
-*/
-
-def helpMessage() {
-    log.info nfcoreHeader()
-    log.info"""
-    Usage:
-
-    The typical command for downloading references is as follows:
-
-    nextflow run nf-core/rnafusion/build-ctat.nf -profile [PROFILE] [OPTIONS] --outdir /path/to/output
-
-    Mandatory arguments:
-        --fasta [file]                Path to fasta reference
-        --gtf [file]                  Path to GTF annotation
-        --genome [str]                Either "GRCh37" or "GRCh38" (default)
-        --outdir [path]               Output directory for downloading
-        -profile [str]                Configuration profile [https://github.com/nf-core/configs]
-    """.stripIndent()
-}
-
-/*
- * SET UP CONFIGURATION VARIABLES
- */
-
-// Show help message
-if (params.help) exit 0, helpMessage()
-
-params.fasta = params.genome ? params.genomes[params.genome].fasta ?: null : null
-params.gtf = params.genome ? params.genomes[params.genome].gtf ?: null : null
-
-ch_fasta = Channel.value(file(params.fasta)).ifEmpty{exit 1, "Fasta file not found: ${params.fasta}"}
-ch_gtf = Channel.value(file(params.gtf)).ifEmpty{exit 1, "GTF annotation file not found: ${params.gtf}"}
-
-if (!params.outdir) exit 1, "Output directory not specified!"
-
-// Header log info
-log.info nfcoreHeader()
-def summary = [:]
-summary['Pipeline Name']  = 'nf-core/rnafusion/build-ctat.nf'
-summary['Pipeline Version'] = workflow.manifest.version
-if(workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container"
-summary['Max Resources']    = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job"
-summary['Output dir']   = params.outdir
-summary['User']         = workflow.userName
-log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n")
-log.info "\033[2m----------------------------------------------------\033[0m"
-
-// Check the hostnames against configured profiles
-checkHostname()
-
-/*
-================================================================================
-DOWNLOAD
-================================================================================
-*/
-
-process star_fusion {
-    label 'process_high'
-    label 'process_long'
-    publishDir "${params.outdir}/star-fusion", mode: 'move'
-
-    input:
-    file(fasta) from ch_fasta
-    file(gtf) from ch_gtf
-
-    output:
-    file 'ctat_genome_lib_build_dir/'
-
-    script:
-    def ctat_url = params.genome == "GRCh37" ? "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/CTAT_HumanFusionLib.v0.1.0/CTAT_HumanFusionLib.v0.1.0.dat.gz" : "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.2.0/fusion_lib.Mar2019.dat.gz"
-    """
-    wget -N ftp://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pfam-A.hmm.gz
-    gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
-
-    wget -N ${ctat_url} -O CTAT_HumanFusionLib.dat.gz
-
-    # Dfam
-    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm
-    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f
-    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i
-    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m
-    wget -N --no-check-certificate https://www.dfam.org/releases/Dfam_3.1/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p
-
-    export TMPDIR=/tmp
-    /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl \\
-        --genome_fa ${fasta} \\
-        --gtf ${gtf} \\
-        --annot_filter_rule /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/AnnotFilterRuleLib/AnnotFilterRule.pm \\
-        --fusion_annot_lib CTAT_HumanFusionLib.dat.gz \\
-        --pfam_db Pfam-A.hmm \\
-        --dfam_db homo_sapiens_dfam.hmm \\
-        --CPU ${task.cpus}
-    """
-}
-
-/*
- * Completion
- */
-workflow.onComplete {
-    log.info "[nf-core/rnafusion/build-ctat.nf] Pipeline Complete"
-}
-
-def nfcoreHeader() {
-    // Log colors ANSI codes
-    c_black = params.monochrome_logs ? '' : "\033[0;30m";
-    c_blue = params.monochrome_logs ? '' : "\033[0;34m";
-    c_cyan = params.monochrome_logs ? '' : "\033[0;36m";
-    c_dim = params.monochrome_logs ? '' : "\033[2m";
-    c_green = params.monochrome_logs ? '' : "\033[0;32m";
-    c_purple = params.monochrome_logs ? '' : "\033[0;35m";
-    c_reset = params.monochrome_logs ? '' : "\033[0m";
-    c_white = params.monochrome_logs ? '' : "\033[0;37m";
-    c_yellow = params.monochrome_logs ? '' : "\033[0;33m";
-
-    return """    -${c_dim}--------------------------------------------------${c_reset}-
-                                            ${c_green},--.${c_black}/${c_green},-.${c_reset}
-    ${c_blue}        ___     __   __   __   ___     ${c_green}/,-._.--~\'${c_reset}
-    ${c_blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${c_yellow}}  {${c_reset}
-    ${c_blue}  | \\| |       \\__, \\__/ |  \\ |___     ${c_green}\\`-._,-`-,${c_reset}
-                                            ${c_green}`._,._,\'${c_reset}
-    ${c_purple}  nf-core/rnafusion v${workflow.manifest.version}${c_reset}
-    -${c_dim}--------------------------------------------------${c_reset}-
-    """.stripIndent()
-}
-
-def checkHostname() {
-    def c_reset = params.monochrome_logs ? '' : "\033[0m"
-    def c_white = params.monochrome_logs ? '' : "\033[0;37m"
-    def c_red = params.monochrome_logs ? '' : "\033[1;91m"
-    def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m"
-    if (params.hostnames) {
-        def hostname = "hostname".execute().text.trim()
-        params.hostnames.each { prof, hnames ->
-            hnames.each { hname ->
-                if (hostname.contains(hname) && !workflow.profile.contains(prof)) {
-                    log.error "====================================================\n" +
-                            "  ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" +
-                            "  but your machine hostname is ${c_white}'$hostname'${c_reset}\n" +
-                            "  ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" +
-                            "============================================================"
-                }
-            }
-        }
-    }
-}
diff --git a/conf/modules.config b/conf/modules.config
index 4c874b9f..1bdafe99 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -90,13 +90,13 @@ process {
     ]
     }
 
-    // withName: FUSIONCATCHER_DOWNLOAD {
-    //     ext.args = "--continue --tries=42"
-    // }
-
-    // withName: FUSIONCATCHER {
-        // ext.args = "--continue --tries=42"
-    // }
+    withName: FUSIONCATCHER_DOWNLOAD {
+        publishDir = [
+            path: { "${params.genomes_base}/fusioncatcher" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
 
     withName: KALLISTO_INDEX {
         ext.args = '-k 31'
diff --git a/containers/fusioncatcher/Dockerfile b/containers/fusioncatcher/Dockerfile
index b713f35c..77efaf57 100644
--- a/containers/fusioncatcher/Dockerfile
+++ b/containers/fusioncatcher/Dockerfile
@@ -1,14 +1,49 @@
-FROM nfcore/base:1.7
+FROM ubuntu:18.04
 
-LABEL authors="Martin Proks" \
-    description="Docker image containing all requirements for nfcore/rnafusion pipeline"
+LABEL Description="This image is used to run FusionCatcher" Version="1.33"
 
-# Install the conda environment
-COPY environment.yml /
-RUN conda env create -f /environment.yml && conda clean -a
+RUN apt-get -y clean \
+    && apt-get -y update \
+    && apt-get -y install \
+    automake \
+    build-essential \
+    bzip2 \
+    cmake \
+    curl \
+    g++ \
+    gawk \
+    gcc \
+    gzip  \
+    libc6-dev \
+    libncurses5-dev \
+    libtbb2 \
+    libtbb-dev \
+    make \
+    parallel \
+    pigz \
+    python \
+    python-dev \
+    python-biopython \
+    python-numpy \
+    python-openpyxl \
+    python-xlrd \
+    tar \
+    unzip \
+    wget \
+    zip \
+    zlib1g \
+    zlib1g-dev \
+    zlibc \
+    default-jdk \
+    && apt-get -y clean
 
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-rnafusion-fusioncatcher_1.20/bin:$PATH
+WORKDIR /opt
 
-# Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-rnafusion-fusioncatcher_1.20 > nf-core-rnafusion-fusioncatcher_1.20.yml
+######################
+## INSTALLATION
+######################
+
+RUN wget --no-check-certificate http://sf.net/projects/fusioncatcher/files/bootstrap.py -O bootstrap.py \
+    && python bootstrap.py -t -y -i /opt/fusioncatcher/v1.33/
+
+ENV PATH /opt/fusioncatcher/v1.33/bin:$PATH
diff --git a/containers/fusioncatcher/environment.yml b/containers/fusioncatcher/environment.yml
deleted file mode 100644
index 1d6a105d..00000000
--- a/containers/fusioncatcher/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-name: nf-core-rnafusion-fusioncatcher_1.20
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::fusioncatcher=1.20
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 8921c7f6..3c6c0ca4 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -4,11 +4,9 @@ process FUSIONCATCHER {
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        // container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.30--hdfd78af_1"
-        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_2"
+        container "docker.io/clinicalgenomics/fusioncatcher:1.33"
     } else {
-        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.30--0"
-        // container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_1"
+        container "docker.io/clinicalgenomics/fusioncatcher:1.33"
     }
 
     input:
@@ -21,6 +19,9 @@ process FUSIONCATCHER {
     tuple val(meta), path("*.fusioncatcher.log")                               , emit: log
     path "versions.yml"                                                        , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
@@ -33,15 +34,15 @@ process FUSIONCATCHER {
         -o . \\
         --skip-blat \\
         $args
+
+    mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher.fusion-genes.txt
+    mv summary_candidate_fusions.txt ${prefix}.fusioncatcher.summary.txt
+    mv fusioncatcher.log ${prefix}.fusioncatcher.log
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1)| sed 's/fusioncatcher.py //')
+    END_VERSIONS
     touch versions.yml
     """
 }
-
-
-    // mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher.fusion-genes.txt
-    // mv summary_candidate_fusions.txt ${prefix}.fusioncatcher.summary.txt
-    // mv fusioncatcher.log ${prefix}.fusioncatcher.log
-    //  fusioncatcher --version | sed 's/fusioncatcher.py //' > versions.yml
-    // touch ${prefix}.fusioncatcher.fusion-genes.txt
-    // touch ${prefix}.fusioncatcher.summary.txt
-    // touch ${prefix}.fusioncatcher.log
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 76a7b8ef..da0faf5f 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -4,9 +4,10 @@ process FUSIONCATCHER_DOWNLOAD {
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_1"
+
+        container "docker.io/clinicalgenomics/fusioncatcher:1.33"
     } else {
-        container "quay.io/biocontainers/fusioncatcher:1.33--hdfd78af_1"
+        container "docker.io/clinicalgenomics/fusioncatcher:1.33"
     }
 
     output:
@@ -33,8 +34,10 @@ process FUSIONCATCHER_DOWNLOAD {
             -o human_${human_version} \\
             $args2
     fi
-    ln -s human_v102 current
 
-    fusioncatcher --version | sed 's/fusioncatcher.py //' > versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
+    END_VERSIONS
     """
 }
diff --git a/nextflow.config b/nextflow.config
index d14fc8ce..14be0540 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -43,12 +43,13 @@ params {
     squid                      = false
     starindex                  = false
     starfusion                 = false
+    fusionreport               = false
 
     // Path to references
     arriba_ref                    = "${params.genomes_base}/arriba"
     ensembl_ref                   = "${params.genomes_base}/ensembl"
     ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
-    fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
+    fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v102"
     pizzly_ref                    = "${params.genomes_base}/pizzly"
     squid_ref                     = "${params.genomes_base}/squid"
     starfusion_ref                = "${params.genomes_base}/starfusion"
@@ -58,6 +59,10 @@ params {
 
     // Path to fusion outputs
     arriba_fusions                = null
+    pizzly_fusions                = null
+    squid_fusions                 = null
+    starfusion_fusions            = null
+    fusioncatcher_fusions         = null
 
     // Boilerplate options
     outdir                     = './results'
diff --git a/nextflow_schema.json b/nextflow_schema.json
index ced1f1dc..b26dcf34 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -108,11 +108,22 @@
                     "description": "Build or run fusioncatcher references/analyses",
                     "default": "false"
                 },
+                "fusioncatcher_fusions": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to fusioncatcher output"
+                },
                 "fusioncatcher_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
+                "fusionreport": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build fusionreport references",
+                    "default": "false"
+                },
                 "fusionreport_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -124,6 +135,11 @@
                     "description": "Build or run pizzly references/analyses",
                     "default": "false"
                 },
+                "pizzly_fusions": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to pizzly output"
+                },
                 "pizzly_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -135,6 +151,11 @@
                     "description": "Build or run squid references/analyses",
                     "default": "false"
                 },
+                "squid_fusions": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to squid output"
+                },
                 "squid_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -146,6 +167,11 @@
                     "description": "Build or run starfusion references/analyses",
                     "default": "false"
                 },
+                "starfusion_fusions": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to starfusion output",
+                },
                 "starfusion_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
diff --git a/subworkflows/local/fusioncatcher_workflow.nf b/subworkflows/local/fusioncatcher_workflow.nf
new file mode 100644
index 00000000..416aecd7
--- /dev/null
+++ b/subworkflows/local/fusioncatcher_workflow.nf
@@ -0,0 +1,38 @@
+//
+// Check input samplesheet and get read channels
+//
+
+include { FUSIONCATCHER }          from '../../modules/local/fusioncatcher/detect/main'
+include { GET_PATH }               from '../../modules/local/getpath/main'
+
+
+workflow FUSIONCATCHER_WORKFLOW {
+    take:
+        reads
+        fast
+
+    main:
+        ch_versions = Channel.empty()
+        ch_dummy_file = file("$baseDir/assets/dummy_file_fusioncatcher.txt", checkIfExists: true)
+
+        if (params.fusioncatcher) {
+            if (params.fusioncatcher_fusions){
+                ch_fusioncatcher_fusions = params.fusioncatcher_fusions
+            } else {
+                FUSIONCATCHER (
+                    INPUT_CHECK.out.reads,
+                    params.fusioncatcher_ref
+                )
+                GET_PATH(FUSIONCATCHER.out.fusions)
+                ch_fusioncatcher_fusions = GET_PATH.out.file
+            }
+        }
+        else {
+            ch_fusioncatcher_fusions = ch_dummy_file
+        }
+
+    emit:
+        fusions  = ch_squid_fusions
+        versions = ch_versions.ifEmpty(null)
+    }
+
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index bceac718..7a4e9d80 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -175,20 +175,19 @@ workflow RNAFUSION {
 
 
 //Run STAR fusion
-
     STARFUSION_WORKFLOW (
         ch_cat_fastq
     )
     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
-    //Run FusionCatcher
-    // if (params.fusioncatcher){
-    //     FUSIONCATCHER (
-    //         INPUT_CHECK.out.reads,
-    //         params.fusioncatcher_ref
-    //     )
-    // }
+//Run fusioncatcher
+
+    FUSIONCATCHER_WORKFLOW (
+        ch_cat_fastq
+    )
+    ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions.first().ifEmpty(null))
+
 
 
 

From 36a28eee28f21bb494e2735843cee796e8dd8342 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 1 Mar 2022 10:59:59 +0100
Subject: [PATCH 170/359] bug fixes in fusioncatcher/starfusion and add
 fusioninspector

---
 modules/local/fusioninspector/main.nf         | 37 +++++++++++++++++++
 modules/local/fusioninspector/meta.yml        | 27 ++++++++++++++
 subworkflows/local/fusioncatcher_workflow.nf  |  5 +--
 .../local/fusioninspector_workflow.nf         | 25 +++++++++++++
 subworkflows/local/starfusion_workflow.nf     |  9 +++--
 workflows/rnafusion.nf                        | 15 ++++++--
 6 files changed, 108 insertions(+), 10 deletions(-)
 create mode 100644 modules/local/fusioninspector/main.nf
 create mode 100644 modules/local/fusioninspector/meta.yml
 create mode 100644 subworkflows/local/fusioninspector_workflow.nf

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
new file mode 100644
index 00000000..53da136a
--- /dev/null
+++ b/modules/local/fusioninspector/main.nf
@@ -0,0 +1,37 @@
+process FUSIONINSPECTOR {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "docker.io/trinityctat/starfusion:1.10.1"
+    } else {
+        container "docker.io/trinityctat/starfusion:1.10.1"
+    }
+
+    input:
+    tuple val(meta), path(reads)
+    path fusion_list
+    path index
+
+    output:
+    path "*"                , emit: output
+    path "versions.yml"     , emit: versions
+
+    script:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def fasta = params.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
+    def args = task.ext.args ?: ''
+    """
+    FusionInspector \\
+        --fusions ${fi_input_list} \\
+        --genome_lib ${reference} \\
+        $fasta \\
+        --CPU ${task.cpus} \\
+        -O . \\
+        --out_prefix $prefix \\
+        --vis $args
+
+    echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > versions.yml
+    """
+}
diff --git a/modules/local/fusioninspector/meta.yml b/modules/local/fusioninspector/meta.yml
new file mode 100644
index 00000000..37f71f06
--- /dev/null
+++ b/modules/local/fusioninspector/meta.yml
@@ -0,0 +1,27 @@
+name: starfusion_downloadgenome
+description: Download STAR-fusion genome resource required to run STAR-Fusion caller
+keywords:
+  - downoad
+tools:
+  - star-fusion:
+      description: Fusion calling algorithm for RNAseq data
+      homepage: https://github.com/STAR-Fusion/
+      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki/installing-star-fusion
+      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion
+      doi: "10.1186/s13059-019-1842-9"
+      licence: ['GPL v3']
+
+input:
+  - genome_version:
+      type: value
+      description: Genome version - either GRCh38 or GRCh38
+      pattern: "GRCh3*"
+
+output:
+  - reference:
+      type: directory
+      description: Genome resource path
+      pattern: "star-fusion-genome"
+
+authors:
+  - "@praveenraj2018"
diff --git a/subworkflows/local/fusioncatcher_workflow.nf b/subworkflows/local/fusioncatcher_workflow.nf
index 416aecd7..28201875 100644
--- a/subworkflows/local/fusioncatcher_workflow.nf
+++ b/subworkflows/local/fusioncatcher_workflow.nf
@@ -9,7 +9,6 @@ include { GET_PATH }               from '../../modules/local/getpath/main'
 workflow FUSIONCATCHER_WORKFLOW {
     take:
         reads
-        fast
 
     main:
         ch_versions = Channel.empty()
@@ -20,7 +19,7 @@ workflow FUSIONCATCHER_WORKFLOW {
                 ch_fusioncatcher_fusions = params.fusioncatcher_fusions
             } else {
                 FUSIONCATCHER (
-                    INPUT_CHECK.out.reads,
+                    reads,
                     params.fusioncatcher_ref
                 )
                 GET_PATH(FUSIONCATCHER.out.fusions)
@@ -32,7 +31,7 @@ workflow FUSIONCATCHER_WORKFLOW {
         }
 
     emit:
-        fusions  = ch_squid_fusions
+        fusions  = ch_fusioncatcher_fusions
         versions = ch_versions.ifEmpty(null)
     }
 
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
new file mode 100644
index 00000000..aeb5bbb5
--- /dev/null
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -0,0 +1,25 @@
+//
+// Check input samplesheet and get read channels
+//
+
+include { FUSIONINSPECTOR }                           from '../../modules/local/fusioninspector/main'
+
+
+workflow FUSIONINSPECTOR_WORKFLOW {
+    take:
+        reads
+        fusion_list
+
+    main:
+        ch_versions = Channel.empty()
+        index ="${params.starfusion_ref}/ctat_genome_lib_build_dir"
+
+        FUSIONINSPECTOR( reads, fusion_list , index )
+        ch_versions = ch_versions.mix(FUSIONINSPECTOR.out.versions)
+
+    emit:
+        versions        = ch_versions.ifEmpty(null)
+        // fusion_list     = FUSIONINSPECTOR.out.fusion_list
+
+}
+
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index 4613ffe7..568df66e 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -4,6 +4,7 @@
 
 include { STAR_ALIGN as STAR_FOR_STARFUSION }    from '../../modules/nf-core/modules/star/align/main'
 include { STARFUSION }                           from '../../modules/local/starfusion/detect/main'
+include { GET_PATH }                         from '../../modules/local/getpath/main'
 
 
 workflow STARFUSION_WORKFLOW {
@@ -19,18 +20,18 @@ workflow STARFUSION_WORKFLOW {
                 ch_starfusion_fusions = params.starfusion_fusions
             } else {
                 gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
-                index ="${params.starfusion_ref}/ctat_genome_lib_build_dir"
+                ref ="${params.starfusion_ref}/ctat_genome_lib_build_dir"
 
                 star_ignore_sjdbgtf = false
                 seq_platform = false
                 seq_center = false
 
-                STAR_FOR_STARFUSION( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+                STAR_FOR_STARFUSION( reads, params.starindex_ref, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
                 reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
 
-                STARFUSION( reads_junction, starfusion_ref)
-                ch_versions = ch_versions.mix(STARFUSION.out.versions, params.starfusion_ref )
+                STARFUSION( reads_junction, ref)
+                ch_versions = ch_versions.mix(STARFUSION.out.versions)
 
                 GET_PATH(STARFUSION.out.fusions)
                 ch_starfusion_fusions = GET_PATH.out.file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 7a4e9d80..d0e661b3 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -54,6 +54,8 @@ include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_w
 include { PIZZLY_WORKFLOW               }   from '../subworkflows/local/pizzly_workflow'
 include { SQUID_WORKFLOW                }   from '../subworkflows/local/squid_workflow'
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
+include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
+include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
 include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
 
 /*
@@ -182,15 +184,12 @@ workflow RNAFUSION {
 
 
 //Run fusioncatcher
-
     FUSIONCATCHER_WORKFLOW (
         ch_cat_fastq
     )
     ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
-
-
      //Run fusion-report
     FUSIONREPORT_WORKFLOW (
         ch_cat_fastq,
@@ -200,6 +199,16 @@ workflow RNAFUSION {
         SQUID_WORKFLOW.out.fusions,
         STARFUSION_WORKFLOW.out.fusions
     )
+
+
+     //Run fusionInpector
+    FUSIONINSPECTOR_WORKFLOW (
+        ch_cat_fastq,
+        FUSIONREPORT_WORKFLOW.out.fusion_list
+    )
+
+
+
 }
 
 

From 460e6c5826dd82534322f8ad4a246ecae021650c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 1 Mar 2022 11:46:36 +0100
Subject: [PATCH 171/359] fixes in fusioncatcher dependency with fusionreport

---
 modules/local/fusionreport/detect/main.nf   | 13 +++++++++----
 subworkflows/local/fusionreport_workflow.nf |  4 +++-
 workflows/rnafusion.nf                      |  5 ++++-
 3 files changed, 16 insertions(+), 6 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index c6397929..4e17b097 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -16,16 +16,21 @@ process FUSIONREPORT {
     path(pizzly_fusions)
     path(squid_fusions)
     path(starfusion_fusions)
+    path(fusioncatcher_fusions)
 
     output:
     path "versions.yml"           , emit: versions
     tuple val(meta), path("*.tsv") , emit: fusion_list
 
     script:
-    def tools = params.arriba      ? "--arriba ${arriba_fusions} " : ''
-    tools    += params.pizzly      ? "--pizzly ${pizzly_fusions} " : ''
-    tools    += params.squid       ? "--squid ${squid_fusions} " : ''
-    tools    += params.starfusion  ? "--squid ${starfusion_fusions} " : ''
+    def tools = params.arriba         ? "--arriba ${arriba_fusions} " : ''
+    tools    += params.pizzly         ? "--pizzly ${pizzly_fusions} " : ''
+    tools    += params.squid          ? "--squid ${squid_fusions} " : ''
+    tools    += params.starfusion     ? "--starfusion ${starfusion_fusions} " : ''
+    tools    += params.fusioncatcher  ? "--fusioncatcher ${fusioncatcher_fusions} " : ''
+
+
+
     """
     fusion_report run $meta.id . $fusionreport_ref $tools --allow-multiple-gene-symbols
 
diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index 3083ff38..fd2e41df 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -13,10 +13,12 @@ workflow FUSIONREPORT_WORKFLOW {
         pizzly_fusions
         squid_fusions
         starfusion_fusions
+        fusioncatcher_fusions
+
     main:
         ch_versions = Channel.empty()
 
-        FUSIONREPORT( reads, fusionreport_ref, arriba_fusions, pizzly_fusions, squid_fusions, starfusion_fusions )
+        FUSIONREPORT( reads, fusionreport_ref, arriba_fusions, pizzly_fusions, squid_fusions, starfusion_fusions, fusioncatcher_fusions )
         ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
 
     emit:
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index d0e661b3..8b2ff2d9 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -197,8 +197,10 @@ workflow RNAFUSION {
         ARRIBA_WORKFLOW.out.fusions,
         PIZZLY_WORKFLOW.out.fusions,
         SQUID_WORKFLOW.out.fusions,
-        STARFUSION_WORKFLOW.out.fusions
+        STARFUSION_WORKFLOW.out.fusions,
+        FUSIONCATCHER_WORKFLOW.out.fusions
     )
+    ch_versions = ch_versions.mix(FUSIONINREPORT_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
      //Run fusionInpector
@@ -206,6 +208,7 @@ workflow RNAFUSION {
         ch_cat_fastq,
         FUSIONREPORT_WORKFLOW.out.fusion_list
     )
+    ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
 

From 8fccb6559e218233fd68fdd17fd5b6df8bb56c98 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 1 Mar 2022 16:18:13 +0100
Subject: [PATCH 172/359] add meta.yml files

---
 modules/local/arriba/download/meta.yml | 26 +++++++++++++
 modules/local/kallisto/quant/meta.yml  | 34 +++++++++++++++++
 modules/local/pizzly/detect/meta.yml   | 44 ++++++++++++++++++++++
 modules/local/squid/annotate/meta.yml  | 36 ++++++++++++++++++
 modules/local/squid/detect/meta.yml    | 42 +++++++++++++++++++++
 scripts/build.sh                       | 51 --------------------------
 subworkflows/nf-core/arriba.nf         | 50 -------------------------
 7 files changed, 182 insertions(+), 101 deletions(-)
 create mode 100644 modules/local/arriba/download/meta.yml
 create mode 100644 modules/local/kallisto/quant/meta.yml
 create mode 100644 modules/local/pizzly/detect/meta.yml
 create mode 100644 modules/local/squid/annotate/meta.yml
 create mode 100644 modules/local/squid/detect/meta.yml
 delete mode 100755 scripts/build.sh
 delete mode 100644 subworkflows/nf-core/arriba.nf

diff --git a/modules/local/arriba/download/meta.yml b/modules/local/arriba/download/meta.yml
new file mode 100644
index 00000000..55e50b11
--- /dev/null
+++ b/modules/local/arriba/download/meta.yml
@@ -0,0 +1,26 @@
+name: arriba_download
+description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
+keywords:
+  - fusion
+  - arriba
+tools:
+  - arriba:
+      description: Fast and accurate gene fusion detection from RNA-Seq data
+      homepage: https://github.com/suhrig/arriba
+      documentation: https://arriba.readthedocs.io/en/latest/
+      tool_dev_url: https://github.com/suhrig/arriba
+      doi: "10.1101/gr.257246.119"
+      licence: ["MIT"]
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - reference:
+      type: directory
+      description: Folder with arriba references
+      pattern: "*"
+
+authors:
+  - "@praveenraj2018, @rannick"
diff --git a/modules/local/kallisto/quant/meta.yml b/modules/local/kallisto/quant/meta.yml
new file mode 100644
index 00000000..836bf9ff
--- /dev/null
+++ b/modules/local/kallisto/quant/meta.yml
@@ -0,0 +1,34 @@
+name: kallisto_quant
+description: runs the kallisto quantification algorithm
+  - quant
+tools:
+  - kallisto:
+      description: Quantifying abundances of transcripts from bulk and single-cell RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads.
+      homepage: https://pachterlab.github.io/kallisto/
+      documentation: https://pachterlab.github.io/kallisto/manual
+      tool_dev_url: https://github.com/pachterlab/kallisto
+      doi: ""
+      licence: ['BSD-2-Clause']
+
+input:
+  - fasta:
+      type: file
+      description: genome fasta file
+      pattern: "*.{fasta*}"
+  - reference:
+      type: directory
+      description: Path to kallisto index
+      pattern: "*"
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - fusions:
+      type: file
+      description: fusions
+      pattern: "*.txt"
+
+authors:
+  - "@rannick"
diff --git a/modules/local/pizzly/detect/meta.yml b/modules/local/pizzly/detect/meta.yml
new file mode 100644
index 00000000..97bf8f09
--- /dev/null
+++ b/modules/local/pizzly/detect/meta.yml
@@ -0,0 +1,44 @@
+name: pizzly
+description: Pizzly detection of fusions.
+keywords:
+  - fusion
+  - pizzly
+tools:
+  - pizzly:
+      description: Fast fusion detection using kallisto
+      homepage: https://github.com/pmelsted/pizzly
+      documentation: https://github.com/pmelsted/pizzly
+      tool_dev_url: https://github.com/pmelsted/pizzly
+      doi: ""
+      licence: ['BSD-2-Clause']
+
+input:
+  - fasta:
+      type: file
+      description: genome fasta file
+      pattern: "*.{fasta*}"
+  - reference:
+      type: directory
+      description: Path to kallisto index
+      pattern: "*"
+  - gtf:
+      type: file
+      description: gtf reference
+      pattern: "*.gtf"
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - fusions:
+      type: file
+      description: fusions
+      pattern: "*pizzly.txt"
+  - unfiltered:
+      type: file
+      description: unfiltered fusions
+      pattern: "*unfiltered.json"
+
+authors:
+  - "@rannick"
diff --git a/modules/local/squid/annotate/meta.yml b/modules/local/squid/annotate/meta.yml
new file mode 100644
index 00000000..ed5e6048
--- /dev/null
+++ b/modules/local/squid/annotate/meta.yml
@@ -0,0 +1,36 @@
+name: squid
+description: Squid detection of fusions.
+keywords:
+  - fusion
+  - pizzly
+tools:
+  - pizzly:
+      description: Fusion detection using squid
+      homepage: https://github.com/Kingsford-Group/squid
+      documentation: https://github.com/Kingsford-Group/squid
+      tool_dev_url: https://github.com/Kingsford-Group/squid
+      doi: ""
+      licence: ['BSD-3-Clause']
+
+input:
+  - fusions:
+      type: directory
+      description: Path to squid fusions
+      pattern: "*.txt"
+  - gtf:
+      type: file
+      description: gtf reference
+      pattern: "*.gtf"
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - fusions_annotated:
+      type: file
+      description: squid fusions annotated
+      pattern: "*squid.fusions.annotated.txt"
+
+authors:
+  - "@rannick"
diff --git a/modules/local/squid/detect/meta.yml b/modules/local/squid/detect/meta.yml
new file mode 100644
index 00000000..db1cc2bb
--- /dev/null
+++ b/modules/local/squid/detect/meta.yml
@@ -0,0 +1,42 @@
+name: squid
+description: Squid detection of fusions.
+keywords:
+  - fusion
+  - pizzly
+tools:
+  - pizzly:
+      description: Fusion detection using squid
+      homepage: https://github.com/Kingsford-Group/squid
+      documentation: https://github.com/Kingsford-Group/squid
+      tool_dev_url: https://github.com/Kingsford-Group/squid
+      doi: ""
+      licence: ['BSD-3-Clause']
+
+input:
+
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - chimeric_bam:
+      type: file
+      description: BAM/CRAM/SAM file containing only chimeric sorted reads
+      pattern: "*.{bam,cram,sam}"
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - fusions:
+      type: directory
+      description: Path to squid fusions
+      pattern: "*.txt"
+
+authors:
+  - "@rannick"
diff --git a/scripts/build.sh b/scripts/build.sh
deleted file mode 100755
index 626542e5..00000000
--- a/scripts/build.sh
+++ /dev/null
@@ -1,51 +0,0 @@
-#!/bin/bash
-
-PREFIX="nfcore/rnafusion"
-
-create_container() {
-    TOOL_PATH=$1
-    VERSION="$(cat $TOOL_PATH/environment.yml | grep "name:" | cut -d":" -f2 | cut -d "_" -f2)"
-    TOOL_NAME=`basename $TOOL_PATH`
-    CONTAINER_NAME="${PREFIX}:${TOOL_NAME}_${VERSION}"
-    echo "Building [$CONTAINER_NAME]"
-    docker build $TOOL_PATH -t $CONTAINER_NAME
-    docker push $CONTAINER_NAME
-}
-
-if [ $# -eq 0 ]; then
-    echo "No tool name specified!"
-    echo "Run scripts/build.sh -h for help"
-    exit 1
-fi
-
-if [ $1 == "-h" ]; then
-    echo "Utility for building docker containers from tools/"
-    echo "Usage: scripts/build.sh [options]"
-    echo
-    echo "Options:"
-    echo "  all             build all tools including main image"
-    echo "  <tool name>     builds specific tool"
-    echo "                  Example: sh scripts/build.sh ericscript"
-    exit 0
-fi
-
-if [ $1 == "all" ]; then
-    for TOOL in containers/*/; do
-        create_container `pwd`/$TOOL ${TOOL%?}
-    done
-    # Build main container
-    VERSION="$(cat nextflow.config | grep -m1 "container" | cut -d":" -f2 | cut -d "'" -f1)"
-    CONTAINER_NAME=$PREFIX:$VERSION
-    echo "Building [$CONTAINER_NAME]"
-    docker build . -t $CONTAINER_NAME
-    docker push $CONTAINER_NAME
-else
-    TOOL=$1
-    TOOL_PATH="$(pwd)/containers/$TOOL"
-    if [ ! -d $TOOL_PATH ]; then
-        echo "The tool doesn't exist"
-        exit 1
-    else
-        create_container $TOOL_PATH
-    fi
-fi
diff --git a/subworkflows/nf-core/arriba.nf b/subworkflows/nf-core/arriba.nf
deleted file mode 100644
index 3c9da480..00000000
--- a/subworkflows/nf-core/arriba.nf
+++ /dev/null
@@ -1,50 +0,0 @@
-/*
- * Fusion calling with STAR and Arriba
- */
-
-params.star_align_options = [:]
-params.arriba_options = [:]
-
-include { STAR_ALIGN }  from '../../modules/nf-core/modules/star/align/main.nf'    addParams( options: params.star_align_options)
-include { ARRIBA }      from '../../modules/nf-core/modules/arriba/main.nf'        addParams( options: params.arriba_options )
-
-workflow FUSION_STAR_ARRIBA {
-    take:
-    reads           // channel: [ val(meta), [ reads ] ]
-    star_index      // channel: STAR index
-    genome_fasta    // channel: genome FASTA file
-    genome_gtf      // channel: genome GTF file
-
-    main:
-
-    //RNAseq alignment with STAR
-    ch_genome_bam = Channel.empty()
-    ch_star_version = Channel.empty()
-    STAR_ALIGN (
-        reads,
-        star_index,
-        genome_gtf
-    )
-    ch_genome_bam   = STAR_ALIGN.out.bam
-    ch_star_version = STAR_ALIGN.out.version
-
-    //Fusion calling with Arriba
-    ch_arriba_fusions        = Channel.empty()
-    ch_arriba_failed_fusions = Channel.empty()
-    ch_arriba_version        = Channel.empty()
-    ARRIBA (
-        ch_genome_bam,
-        genome_fasta,
-        genome_gtf
-    )
-    ch_arriba_fusions        = ARRIBA.out.fusions
-    ch_arriba_failed_fusions = ARRIBA.out.fusions_fail
-    ch_arriba_version        = ARRIBA.out.version
-
-    emit:
-    bam          = ch_genome_bam            // channel: [ val(meta), [ bam ] ]
-    fusions      = ch_arriba_fusions        // channel: [ val(meta), [ fusions ]
-    fusions_fail = ch_arriba_failed_fusions // channel: [ val(meta), [ fusions_fail ]
-    ch_star_version
-    ch_arriba_version
-}

From cbf24120344c57d56afa642f2e26e8026efdde07 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 1 Mar 2022 16:19:54 +0100
Subject: [PATCH 173/359] clean-up meta.yml files, change output of
 versions.yml in scripts, formatting

---
 modules/local/arriba/download/main.nf         |  5 ++-
 modules/local/ensembl/main.nf                 |  7 +++-
 modules/local/fusioncatcher/detect/main.nf    |  8 ++--
 modules/local/fusioncatcher/detect/meta.yml   |  6 +--
 modules/local/fusioncatcher/download/meta.yml | 12 ++----
 modules/local/fusioninspector/meta.yml        | 40 ++++++++++++-------
 modules/local/fusionreport/detect/meta.yml    | 34 ++++++++++++----
 7 files changed, 71 insertions(+), 41 deletions(-)

diff --git a/modules/local/arriba/download/main.nf b/modules/local/arriba/download/main.nf
index d7620345..b55492e7 100644
--- a/modules/local/arriba/download/main.nf
+++ b/modules/local/arriba/download/main.nf
@@ -19,7 +19,10 @@ process ARRIBA_DOWNLOAD {
     tar -xzvf arriba_v2.1.0.tar.gz
     rm arriba_v2.1.0.tar.gz
 
-    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusion_report: \$(echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3)
+    END_VERSIONS
     """
 
     stub:
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 0d2eec43..1b689f40 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -1,5 +1,5 @@
 process ENSEMBL_DOWNLOAD {
-    tag "ensembl $ensembl_version"
+    tag "ensembl"
     label 'process_low'
 
     conda (params.enable_conda ? "bioconda::gnu-wget=1.18" : null)
@@ -32,7 +32,10 @@ process ENSEMBL_DOWNLOAD {
     gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
     gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
 
-    echo \$(wget -V 2>&1) | grep "GNU Wget" | cut -d" " -f3 > versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+    END_VERSIONS
     """
 
     stub:
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 3c6c0ca4..480b9f9d 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -14,10 +14,10 @@ process FUSIONCATCHER {
     path reference
 
     output:
-    tuple val(meta), path("*.fusioncatcher.fusion-genes.txt")   , optional:true, emit: fusions
-    tuple val(meta), path("*.fusioncatcher.summary.txt")        , optional:true, emit: summary
-    tuple val(meta), path("*.fusioncatcher.log")                               , emit: log
-    path "versions.yml"                                                        , emit: versions
+    tuple val(meta), path("*.fusioncatcher.fusion-genes.txt")   , optional:true  , emit: fusions
+    tuple val(meta), path("*.fusioncatcher.summary.txt")        , optional:true  , emit: summary
+    tuple val(meta), path("*.fusioncatcher.log")                                 , emit: log
+    path "versions.yml"                                                          , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/local/fusioncatcher/detect/meta.yml b/modules/local/fusioncatcher/detect/meta.yml
index 4f80fa58..8081142b 100644
--- a/modules/local/fusioncatcher/detect/meta.yml
+++ b/modules/local/fusioncatcher/detect/meta.yml
@@ -24,7 +24,7 @@ input:
   - reference:
       type: directory
       description: Path to fusioncatcher references
-      pattern: "fusioncatcher-genome"
+      pattern: "*"
 
 output:
   - meta:
@@ -32,7 +32,7 @@ output:
       description: |
         Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
-  - fusiongenes:
+  - fusions:
       type: file
       description: Final list of candidate fusion genes
       pattern: "*.fusioncatcher.fusion-genes.txt"
@@ -50,4 +50,4 @@ output:
         pattern: "versions.yml"
 
 authors:
-  - "@praveenraj2018"
+  - "@praveenraj2018. @rannick"
diff --git a/modules/local/fusioncatcher/download/meta.yml b/modules/local/fusioncatcher/download/meta.yml
index 128e8f88..b24b5e82 100644
--- a/modules/local/fusioncatcher/download/meta.yml
+++ b/modules/local/fusioncatcher/download/meta.yml
@@ -11,12 +11,6 @@ tools:
       doi: "10.1101/011650"
       licence: ['GPL v3']
 
-input:
-  - organism:
-      type: value
-      description: Organism for which the data is downloaded from Ensembl database and built
-      pattern: "*"
-
 output:
   - versions:
         type: file
@@ -24,8 +18,8 @@ output:
         pattern: "versions.yml"
   - reference:
       type: directory
-      description: directory containing the genome resource files required for fusioncatcher
-      pattern: "fusioncatcher-genome"
+      description: Path to fusioncatcher references
+      pattern: "*"
 
 authors:
-  - "@praveenraj2018"
+  - "@praveenraj2018, @rannick"
diff --git a/modules/local/fusioninspector/meta.yml b/modules/local/fusioninspector/meta.yml
index 37f71f06..18459aac 100644
--- a/modules/local/fusioninspector/meta.yml
+++ b/modules/local/fusioninspector/meta.yml
@@ -1,22 +1,32 @@
-name: starfusion_downloadgenome
-description: Download STAR-fusion genome resource required to run STAR-Fusion caller
+name: fusioninspector
+description: Validation of Fusion Transcript Predictions
 keywords:
-  - downoad
+  - fusioninspector
 tools:
-  - star-fusion:
-      description: Fusion calling algorithm for RNAseq data
-      homepage: https://github.com/STAR-Fusion/
-      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki/installing-star-fusion
-      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion
-      doi: "10.1186/s13059-019-1842-9"
-      licence: ['GPL v3']
+  - fusioninspector:
+      description: Validation of Fusion Transcript Predictions
+      homepage: https://github.com/FusionInspector/FusionInspector
+      documentation: https://github.com/FusionInspector/FusionInspector/wiki
+      tool_dev_url: https://github.com/FusionInspector/FusionInspector
+      doi: 10.1101/2021.08.02.454639"
+      licence: https://github.com/FusionInspector/FusionInspector/blob/master/LICENSE.txt
 
 input:
-  - genome_version:
-      type: value
-      description: Genome version - either GRCh38 or GRCh38
-      pattern: "GRCh3*"
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: FASTQ file
+      pattern: "*.{fastq*}"
+  - reference:
+      type: directory
+      description: Path to ctat references
+      pattern: "*"
 
+# TODO complete output
 output:
   - reference:
       type: directory
@@ -24,4 +34,4 @@ output:
       pattern: "star-fusion-genome"
 
 authors:
-  - "@praveenraj2018"
+  - "@rannick"
diff --git a/modules/local/fusionreport/detect/meta.yml b/modules/local/fusionreport/detect/meta.yml
index dd3faf11..7767b45b 100644
--- a/modules/local/fusionreport/detect/meta.yml
+++ b/modules/local/fusionreport/detect/meta.yml
@@ -5,9 +5,8 @@ keywords:
 tools:
   - fusionreport:
       description: fusionreport
-      homepage: https://github.com/ndaniel/fusioncatcher/
-      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
-      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
+      homepage: https://github.com/matq007/fusion-report
+      documentation: https://matq007.github.io/fusion-report/#/
       doi: "10.1101/011650"
       licence: ['GPL v3']
 
@@ -19,11 +18,27 @@ input:
         e.g. [ id:'test', single_end:false ]
   - reference:
       type: path
-      description: DB
+      description: Path to fusionreport references
       pattern: "*"
-  - fusions:
+  - arriba_fusions:
       type: path
-      description: File contains fusions which pass all of Arriba's filters.
+      description: File
+      pattern: "*.fusions.tsv"
+  - pizzly_fusions:
+      type: path
+      description: File containing fusions from arriba
+      pattern: "*.fusions.tsv"
+  - squid_fusions:
+      type: path
+      description: File containing fusions from squid
+      pattern: "*.fusions.tsv"
+  - starfusion_fusions:
+      type: path
+      description: File containing fusions from STARfusion
+      pattern: "*.fusions.tsv"
+  - fusioncatcher_fusions:
+      type: path
+      description: File containing fusions from fusioncatcher
       pattern: "*.fusions.tsv"
 
 output:
@@ -32,5 +47,10 @@ output:
         description: File containing software versions
         pattern: "versions.yml"
 
+  - fusion_list:
+        type: file
+        description: File containing the summary of all fusions fed-in
+        pattern: "*.tsv"
+
 authors:
-  - "@praveenraj2018"
+  - "@praveenraj2018, @rannick"

From ae6552f72085b5f7c401f86b7b2912c2b13127d4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 1 Mar 2022 16:22:14 +0100
Subject: [PATCH 174/359] fix bug in nextflow_schema.json (trailing comma)
 change output of versions.yml in scripts

---
 modules/local/fusioncatcher/download/main.nf |  2 +-
 modules/local/fusioninspector/main.nf        |  5 ++++-
 modules/local/fusionreport/detect/main.nf    |  4 ++--
 modules/local/fusionreport/download/main.nf  |  8 +++++++-
 modules/local/kallisto/quant/main.nf         |  5 +++--
 modules/local/pizzly/detect/main.nf          |  6 +++---
 modules/local/squid/annotate/main.nf         |  8 ++++----
 modules/local/squid/detect/main.nf           |  6 +++---
 modules/local/starfusion/detect/main.nf      | 13 ++++++++-----
 nextflow_schema.json                         |  2 +-
 10 files changed, 36 insertions(+), 23 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index da0faf5f..119f2523 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -1,5 +1,5 @@
 process FUSIONCATCHER_DOWNLOAD {
-    tag 'fusioncatcher'
+    tag "fusioncatcher_download"
     label 'process_medium'
 
     conda (params.enable_conda ? "bioconda::fusioncatcher=1.33" : null)
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 53da136a..941e9bb8 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -32,6 +32,9 @@ process FUSIONINSPECTOR {
         --out_prefix $prefix \\
         --vis $args
 
-    echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        STAR-Fusion: \$(echo STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
+    END_VERSIONS
     """
 }
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 4e17b097..7406255f 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -1,5 +1,5 @@
 process FUSIONREPORT {
-    tag 'fusionreport'
+    tag "$meta.id"
     label 'process_medium'
 
     // Note: 2.7X indices incompatible with AWS iGenomes.
@@ -30,7 +30,7 @@ process FUSIONREPORT {
     tools    += params.fusioncatcher  ? "--fusioncatcher ${fusioncatcher_fusions} " : ''
 
 
-
+// TODO: mv file to a prefix-including name including
     """
     fusion_report run $meta.id . $fusionreport_ref $tools --allow-multiple-gene-symbols
 
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 6ed8584f..f0e1e57e 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -17,10 +17,16 @@ process FUSIONREPORT_DOWNLOAD {
     path "*"                , emit: reference
     path "versions.yml"     , emit: versions
 
+
+    //TODO: update mitelman to take DB from https://storage.googleapis.com/mitelman-data-files/prod/mitelman_db.zip
+    //TODO: update fusionGDB to GRCh38
     script:
     """
     fusion_report download --cosmic_usr $username --cosmic_passwd $passwd .
 
-    fusion_report --version > versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusion_report: \$(fusion_report --version)
+    END_VERSIONS
     """
 }
diff --git a/modules/local/kallisto/quant/main.nf b/modules/local/kallisto/quant/main.nf
index 8c7fac5c..bc0f3511 100644
--- a/modules/local/kallisto/quant/main.nf
+++ b/modules/local/kallisto/quant/main.nf
@@ -1,5 +1,5 @@
 process KALLISTO_QUANT {
-    tag "kalliso_quant"
+    tag "$meta.id"
     label 'process_medium'
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
@@ -14,7 +14,7 @@ process KALLISTO_QUANT {
 
     output:
     path "versions.yml"                        , emit: versions
-    tuple val(meta), path("fusion.txt")        , emit: txt
+    tuple val(meta), path("*fusions.txt")      , emit: txt
 
     script:
     def args = task.ext.args ?: ''
@@ -26,6 +26,7 @@ process KALLISTO_QUANT {
         --fusion \
         -o . \
         $reads
+    mv fusion.txt ${prefix}.kallisto_quant.fusions.txt
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index d17b02c1..8453456e 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -16,7 +16,7 @@ process PIZZLY {
 
     output:
     path "versions.yml"                              , emit: versions
-    tuple val(meta), path('*_pizzly.txt')            , emit: fusions
+    tuple val(meta), path('*pizzly.txt')            , emit: fusions
     tuple val(meta), path('*unfiltered.json')        , emit: fusions_unfiltered
 
     script:
@@ -27,9 +27,9 @@ process PIZZLY {
         $args \\
         --gtf $gtf \\
         --fasta $transcript \\
-        --output ${prefix}_pizzly $txt
+        --output ${prefix}.pizzly ./fusion.txt
 
-    pizzly_flatten_json.py ${prefix}_pizzly.json ${prefix}_pizzly.txt
+    pizzly_flatten_json.py ${prefix}.pizzly.json ${prefix}.pizzly.txt
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/local/squid/annotate/main.nf b/modules/local/squid/annotate/main.nf
index 9ce03506..585b082d 100644
--- a/modules/local/squid/annotate/main.nf
+++ b/modules/local/squid/annotate/main.nf
@@ -1,6 +1,6 @@
 
 process SQUID_ANNOTATE {
-    tag "squid"
+    tag "$meta.id"
     label 'process_medium'
 
     conda (params.enable_conda ? "bioconda::squid=1.5" : null)
@@ -15,15 +15,15 @@ process SQUID_ANNOTATE {
     path gtf
 
     output:
-    tuple val(meta), path("*_annotated.txt"), emit: fusions_annotated
-    path  "versions.yml"          , emit: versions
+    tuple val(meta), path("*annotated.txt")    , emit: fusions_annotated
+    path  "versions.yml"                        , emit: versions
 
 
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    AnnotateSQUIDOutput.py $gtf $txt ${prefix}_fusions_annotated.txt
+    AnnotateSQUIDOutput.py $gtf $txt ${prefix}.squid.fusions.annotated.txt
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/local/squid/detect/main.nf b/modules/local/squid/detect/main.nf
index 896813c6..80106c0f 100644
--- a/modules/local/squid/detect/main.nf
+++ b/modules/local/squid/detect/main.nf
@@ -14,15 +14,15 @@ process SQUID {
     tuple val(meta), path(bam), path(chimeric_bam)
 
     output:
-    tuple val(meta), path("*_sv.txt"), emit: fusions
-    path  "versions.yml"          , emit: versions
+    tuple val(meta), path("*sv.txt") , emit: fusions
+    path  "versions.yml"             , emit: versions
 
 
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    squid -b $bam -c $chimeric_bam -o ${prefix}_fusions
+    squid -b $bam -c $chimeric_bam -o ${prefix}.squid.fusions
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index a96228bb..851e400d 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -14,10 +14,10 @@ process STARFUSION {
     path reference
 
     output:
-    tuple val(meta), path("*.fusion_predictions.tsv"), emit: fusions
-    tuple val(meta), path("*.abridged.tsv")          , emit: abridged
-    tuple val(meta), path("*.coding_effect.tsv")     , optional: true, emit: coding_effect
-    path "versions.yml"                              , emit: versions
+    tuple val(meta), path("*.fusion_predictions.tsv")                   , emit: fusions
+    tuple val(meta), path("*.abridged.tsv")                             , emit: abridged
+    tuple val(meta), path("*.coding_effect.tsv")     , optional: true   , emit: coding_effect
+    path "versions.yml"                                                 , emit: versions
 
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
@@ -37,7 +37,10 @@ process STARFUSION {
     mv star-fusion.fusion_predictions.abridged.tsv ${prefix}.starfusion.abridged.tsv
     mv star-fusion.fusion_predictions.abridged.coding_effect.tsv ${prefix}.starfusion.abridged.coding_effect.tsv
 
-    echo \$(STAR-Fusion --version 2>&1) | grep -i 'version' | sed 's/STAR-Fusion version: //' > versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        STAR-Fusion: \$(echo STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
+    END_VERSIONS
     """
 
     stub:
diff --git a/nextflow_schema.json b/nextflow_schema.json
index b26dcf34..371b773a 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -170,7 +170,7 @@
                 "starfusion_fusions": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
-                    "description": "Path to starfusion output",
+                    "description": "Path to starfusion output"
                 },
                 "starfusion_ref": {
                     "type": "string",

From b1be063eb1e26da6bd3d6bb9849f5fc22c14cc57 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 1 Mar 2022 17:09:28 +0100
Subject: [PATCH 175/359] linting

---
 modules/local/starfusion/download/main.nf | 2 +-
 workflows/rnafusion.nf                    | 4 ++--
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 13955427..ecc5f7c7 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -21,7 +21,7 @@ process STARFUSION_DOWNLOAD {
     export TMPDIR=/tmp
 
     wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
-    wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz -O CTAT_HumanFusionLib_Mar2021.dat.gz --no-check-certificate 
+    wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz -O CTAT_HumanFusionLib_Mar2021.dat.gz --no-check-certificate
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f --no-check-certificate
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index bb4c68fe..bfe31d29 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -187,7 +187,7 @@ workflow RNAFUSION {
     ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
-     //Run fusion-report
+    //Run fusion-report
     FUSIONREPORT_WORKFLOW (
         ch_cat_fastq,
         params.fusionreport_ref,
@@ -200,7 +200,7 @@ workflow RNAFUSION {
     ch_versions = ch_versions.mix(FUSIONINREPORT_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
-     //Run fusionInpector
+    //Run fusionInpector
     FUSIONINSPECTOR_WORKFLOW (
         ch_cat_fastq,
         FUSIONREPORT_WORKFLOW.out.fusion_list

From 1147bd689b706502643bb9dad0f8a50dd94f1cef Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Tue, 1 Mar 2022 20:11:58 +0100
Subject: [PATCH 176/359] Fix typo in ch_versions

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index bb4c68fe..554b3c9d 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -197,7 +197,7 @@ workflow RNAFUSION {
         STARFUSION_WORKFLOW.out.fusions,
         FUSIONCATCHER_WORKFLOW.out.fusions
     )
-    ch_versions = ch_versions.mix(FUSIONINREPORT_WORKFLOW.out.versions.first().ifEmpty(null))
+    ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
      //Run fusionInpector

From 4a6090b0a0c2eb1bac794b6567c5cfebcd976149 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Tue, 1 Mar 2022 20:19:45 +0100
Subject: [PATCH 177/359] Update fastqc + multiqc modules

---
 modules.json                             |  4 +-
 modules/nf-core/modules/fastqc/meta.yml  | 90 ++++++++++++------------
 modules/nf-core/modules/multiqc/main.nf  |  6 +-
 modules/nf-core/modules/multiqc/meta.yml | 66 ++++++++---------
 4 files changed, 83 insertions(+), 83 deletions(-)

diff --git a/modules.json b/modules.json
index afd3b759..dec440a8 100644
--- a/modules.json
+++ b/modules.json
@@ -13,13 +13,13 @@
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
             "fastqc": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "kallisto/index": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
             "multiqc": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "samtools/faidx": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml
index b09553a3..4da5bb5a 100644
--- a/modules/nf-core/modules/fastqc/meta.yml
+++ b/modules/nf-core/modules/fastqc/meta.yml
@@ -1,52 +1,52 @@
 name: fastqc
 description: Run FastQC on sequenced reads
 keywords:
-    - quality control
-    - qc
-    - adapters
-    - fastq
+  - quality control
+  - qc
+  - adapters
+  - fastq
 tools:
-    - fastqc:
-        description: |
-            FastQC gives general quality metrics about your reads.
-            It provides information about the quality score distribution
-            across your reads, the per base sequence content (%A/C/G/T).
-            You get information about adapter contamination and other
-            overrepresented sequences.
-        homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
-        documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
-        licence: ['GPL-2.0-only']
+  - fastqc:
+      description: |
+        FastQC gives general quality metrics about your reads.
+        It provides information about the quality score distribution
+        across your reads, the per base sequence content (%A/C/G/T).
+        You get information about adapter contamination and other
+        overrepresented sequences.
+      homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
+      documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
+      licence: ["GPL-2.0-only"]
 input:
-    - meta:
-        type: map
-        description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-    - reads:
-        type: file
-        description: |
-            List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-            respectively.
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
 output:
-    - meta:
-        type: map
-        description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-    - html:
-        type: file
-        description: FastQC report
-        pattern: "*_{fastqc.html}"
-    - zip:
-        type: file
-        description: FastQC report archive
-        pattern: "*_{fastqc.zip}"
-    - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - html:
+      type: file
+      description: FastQC report
+      pattern: "*_{fastqc.html}"
+  - zip:
+      type: file
+      description: FastQC report archive
+      pattern: "*_{fastqc.zip}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 authors:
-    - "@drpatelh"
-    - "@grst"
-    - "@ewels"
-    - "@FelixKrueger"
+  - "@drpatelh"
+  - "@grst"
+  - "@ewels"
+  - "@FelixKrueger"
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index 0ff7cac1..1264aac1 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -1,10 +1,10 @@
 process MULTIQC {
     label 'process_medium'
 
-    conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null)
+    conda (params.enable_conda ? 'bioconda::multiqc=1.12' : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.12--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.12--pyhdfd78af_0' }"
 
     input:
     path multiqc_files
diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml
index 63c75a45..6fa891ef 100644
--- a/modules/nf-core/modules/multiqc/meta.yml
+++ b/modules/nf-core/modules/multiqc/meta.yml
@@ -1,40 +1,40 @@
 name: MultiQC
 description: Aggregate results from bioinformatics analyses across many samples into a single report
 keywords:
-    - QC
-    - bioinformatics tools
-    - Beautiful stand-alone HTML report
+  - QC
+  - bioinformatics tools
+  - Beautiful stand-alone HTML report
 tools:
-    - multiqc:
-        description: |
-            MultiQC searches a given directory for analysis logs and compiles a HTML report.
-            It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
-        homepage: https://multiqc.info/
-        documentation: https://multiqc.info/docs/
-        licence: ['GPL-3.0-or-later']
+  - multiqc:
+      description: |
+        MultiQC searches a given directory for analysis logs and compiles a HTML report.
+        It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
+      homepage: https://multiqc.info/
+      documentation: https://multiqc.info/docs/
+      licence: ["GPL-3.0-or-later"]
 input:
-    - multiqc_files:
-        type: file
-        description: |
-            List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+  - multiqc_files:
+      type: file
+      description: |
+        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
 output:
-    - report:
-        type: file
-        description: MultiQC report file
-        pattern: "multiqc_report.html"
-    - data:
-        type: dir
-        description: MultiQC data dir
-        pattern: "multiqc_data"
-    - plots:
-        type: file
-        description: Plots created by MultiQC
-        pattern: "*_data"
-    - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+  - report:
+      type: file
+      description: MultiQC report file
+      pattern: "multiqc_report.html"
+  - data:
+      type: dir
+      description: MultiQC data dir
+      pattern: "multiqc_data"
+  - plots:
+      type: file
+      description: Plots created by MultiQC
+      pattern: "*_data"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 authors:
-    - "@abhi18av"
-    - "@bunop"
-    - "@drpatelh"
+  - "@abhi18av"
+  - "@bunop"
+  - "@drpatelh"

From 490b9f732f184e30c8c0959912e9ee52c4dc2f4d Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Tue, 1 Mar 2022 20:26:54 +0100
Subject: [PATCH 178/359] Updated more recipes that are likely just imported
 entirely

---
 modules.json                                  |  8 +-
 .../nf-core/modules/kallisto/index/meta.yml   |  2 +-
 .../nf-core/modules/samtools/faidx/main.nf    |  6 +-
 .../nf-core/modules/samtools/faidx/meta.yml   | 72 +++++++++---------
 modules/nf-core/modules/samtools/sort/main.nf |  6 +-
 .../nf-core/modules/samtools/sort/meta.yml    | 74 +++++++++----------
 modules/nf-core/modules/samtools/view/main.nf |  6 +-
 7 files changed, 87 insertions(+), 87 deletions(-)

diff --git a/modules.json b/modules.json
index dec440a8..c1ba0c61 100644
--- a/modules.json
+++ b/modules.json
@@ -16,19 +16,19 @@
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "kallisto/index": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "multiqc": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "samtools/faidx": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
             },
             "samtools/sort": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
             },
             "samtools/view": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+                "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
             },
             "star/align": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
diff --git a/modules/nf-core/modules/kallisto/index/meta.yml b/modules/nf-core/modules/kallisto/index/meta.yml
index dd952e33..307650b2 100644
--- a/modules/nf-core/modules/kallisto/index/meta.yml
+++ b/modules/nf-core/modules/kallisto/index/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://pachterlab.github.io/kallisto/manual
       tool_dev_url: https://github.com/pachterlab/kallisto
       doi: ""
-      licence: ['BSD-2-Clause']
+      licence: ["BSD-2-Clause"]
 
 input:
   - fasta:
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf
index b83a4952..7732a4ec 100644
--- a/modules/nf-core/modules/samtools/faidx/main.nf
+++ b/modules/nf-core/modules/samtools/faidx/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_FAIDX {
     tag "$fasta"
     label 'process_low'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.14" : null)
+    conda (params.enable_conda ? "bioconda::samtools=1.15" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' :
-        'quay.io/biocontainers/samtools:1.14--hb421002_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' :
+        'quay.io/biocontainers/samtools:1.15--h1170115_1' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/modules/samtools/faidx/meta.yml b/modules/nf-core/modules/samtools/faidx/meta.yml
index bae97a39..e9767764 100644
--- a/modules/nf-core/modules/samtools/faidx/meta.yml
+++ b/modules/nf-core/modules/samtools/faidx/meta.yml
@@ -1,43 +1,43 @@
 name: samtools_faidx
 description: Index FASTA file
 keywords:
-    - index
-    - fasta
+  - index
+  - fasta
 tools:
-    - samtools:
-        description: |
-            SAMtools is a set of utilities for interacting with and post-processing
-            short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
-            These files are generated as output by short read aligners like BWA.
-        homepage: http://www.htslib.org/
-        documentation: http://www.htslib.org/doc/samtools.html
-        doi: 10.1093/bioinformatics/btp352
-        licence: ['MIT']
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
 input:
-    - meta:
-        type: map
-        description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-    - fasta:
-        type: file
-        description: FASTA file
-        pattern: "*.{fa,fasta}"
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fasta:
+      type: file
+      description: FASTA file
+      pattern: "*.{fa,fasta}"
 output:
-    - meta:
-        type: map
-        description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-    - fai:
-        type: file
-        description: FASTA index file
-        pattern: "*.{fai}"
-    - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fai:
+      type: file
+      description: FASTA index file
+      pattern: "*.{fai}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 authors:
-    - "@drpatelh"
-    - "@ewels"
-    - "@phue"
+  - "@drpatelh"
+  - "@ewels"
+  - "@phue"
diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf
index 0f2237cc..0e2de8ba 100644
--- a/modules/nf-core/modules/samtools/sort/main.nf
+++ b/modules/nf-core/modules/samtools/sort/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_SORT {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.14" : null)
+    conda (params.enable_conda ? "bioconda::samtools=1.15" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' :
-        'quay.io/biocontainers/samtools:1.14--hb421002_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' :
+        'quay.io/biocontainers/samtools:1.15--h1170115_1' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/modules/samtools/sort/meta.yml b/modules/nf-core/modules/samtools/sort/meta.yml
index 3402a068..a820c55a 100644
--- a/modules/nf-core/modules/samtools/sort/meta.yml
+++ b/modules/nf-core/modules/samtools/sort/meta.yml
@@ -1,44 +1,44 @@
 name: samtools_sort
 description: Sort SAM/BAM/CRAM file
 keywords:
-    - sort
-    - bam
-    - sam
-    - cram
+  - sort
+  - bam
+  - sam
+  - cram
 tools:
-    - samtools:
-        description: |
-            SAMtools is a set of utilities for interacting with and post-processing
-            short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
-            These files are generated as output by short read aligners like BWA.
-        homepage: http://www.htslib.org/
-        documentation: hhttp://www.htslib.org/doc/samtools.html
-        doi: 10.1093/bioinformatics/btp352
-        licence: ['MIT']
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: hhttp://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
 input:
-    - meta:
-        type: map
-        description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-    - bam:
-        type: file
-        description: BAM/CRAM/SAM file
-        pattern: "*.{bam,cram,sam}"
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
 output:
-    - meta:
-        type: map
-        description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-    - bam:
-        type: file
-        description: Sorted BAM/CRAM/SAM file
-        pattern: "*.{bam,cram,sam}"
-    - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: Sorted BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 authors:
-    - "@drpatelh"
-    - "@ewels"
+  - "@drpatelh"
+  - "@ewels"
diff --git a/modules/nf-core/modules/samtools/view/main.nf b/modules/nf-core/modules/samtools/view/main.nf
index aee21a4e..75aad063 100644
--- a/modules/nf-core/modules/samtools/view/main.nf
+++ b/modules/nf-core/modules/samtools/view/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_VIEW {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.14" : null)
+    conda (params.enable_conda ? "bioconda::samtools=1.15" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' :
-        'quay.io/biocontainers/samtools:1.14--hb421002_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' :
+        'quay.io/biocontainers/samtools:1.15--h1170115_1' }"
 
     input:
     tuple val(meta), path(input)

From 217dedbd0ff9dd0349d87c18e0316605ff72b18b Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Wed, 2 Mar 2022 09:01:17 +0100
Subject: [PATCH 179/359] Add updates to STAR

---
 modules.json                                         | 4 ++--
 modules/nf-core/modules/star/align/meta.yml          | 2 +-
 modules/nf-core/modules/star/genomegenerate/meta.yml | 2 +-
 3 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules.json b/modules.json
index c1ba0c61..be505243 100644
--- a/modules.json
+++ b/modules.json
@@ -31,10 +31,10 @@
                 "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
             },
             "star/align": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "star/genomegenerate": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             }
         }
     }
diff --git a/modules/nf-core/modules/star/align/meta.yml b/modules/nf-core/modules/star/align/meta.yml
index 2d78b81a..7ee10f1c 100644
--- a/modules/nf-core/modules/star/align/meta.yml
+++ b/modules/nf-core/modules/star/align/meta.yml
@@ -13,7 +13,7 @@ tools:
       homepage: https://github.com/alexdobin/STAR
       manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
       doi: 10.1093/bioinformatics/bts635
-      licence: ['MIT']
+      licence: ["MIT"]
 input:
   - meta:
       type: map
diff --git a/modules/nf-core/modules/star/genomegenerate/meta.yml b/modules/nf-core/modules/star/genomegenerate/meta.yml
index 04ade195..8181157a 100644
--- a/modules/nf-core/modules/star/genomegenerate/meta.yml
+++ b/modules/nf-core/modules/star/genomegenerate/meta.yml
@@ -13,7 +13,7 @@ tools:
       homepage: https://github.com/alexdobin/STAR
       manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
       doi: 10.1093/bioinformatics/bts635
-      licence: ['MIT']
+      licence: ["MIT"]
 input:
   - fasta:
       type: file

From 567d1817ad5bcee333bb651175889762fea677a6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Mar 2022 10:24:19 +0100
Subject: [PATCH 180/359] fix arriba bug: fail without --arriba option

---
 subworkflows/local/arriba_workflow.nf | 17 +++++++++++------
 1 file changed, 11 insertions(+), 6 deletions(-)

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 76db20d5..fd6734da 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -2,9 +2,10 @@
 // Check input samplesheet and get read channels
 //
 
-include { STAR_ALIGN as STAR_FOR_ARRIBA }    from '../../modules/nf-core/modules/star/align/main'
-include { ARRIBA }                           from '../../modules/nf-core/modules/arriba/main'
-include { GET_PATH }                         from '../../modules/local/getpath/main'
+include { STAR_ALIGN as STAR_FOR_ARRIBA }         from '../../modules/nf-core/modules/star/align/main'
+include { ARRIBA }                                from '../../modules/nf-core/modules/arriba/main'
+include { GET_PATH as GET_PATH_ARRIBA }           from '../../modules/local/getpath/main'
+include { GET_PATH as GET_PATH_ARRIBA_FAIL }      from '../../modules/local/getpath/main'
 
 
 workflow ARRIBA_WORKFLOW {
@@ -20,6 +21,7 @@ workflow ARRIBA_WORKFLOW {
         if (params.arriba) {
             if (params.arriba_fusions) {
                 ch_arriba_fusions = params.arriba_fusions
+                ch_arriba_fusion_fail = ch_dummy_file
             } else {
                 gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
                 star_ignore_sjdbgtf = false
@@ -33,17 +35,20 @@ workflow ARRIBA_WORKFLOW {
                 ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
                 ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
-                GET_PATH(ARRIBA.out.fusions)
-                ch_arriba_fusions = GET_PATH.out.file
+                GET_PATH_ARRIBA(ARRIBA.out.fusions)
+                ch_arriba_fusions = GET_PATH_ARRIBA.out.file
+                GET_PATH_ARRIBA(ARRIBA.out.fusions_fail)
+                ch_arriba_fusion_fail = GET_PATH_ARRIBA_FAIL.out.file
             }
         }
         else {
             ch_arriba_fusions = ch_dummy_file
+            ch_arriba_fusion_fail = ch_dummy_file
         }
 
     emit:
         fusions         = ch_arriba_fusions
-        fusions_fail    = ARRIBA.out.fusions_fail
+        fusions_fail    = ch_arriba_fusion_fail
         versions        = ch_versions.ifEmpty(null)
     }
 

From e0e0315829a17d1f8b793ed7aa6402577fd75c32 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Mar 2022 10:48:59 +0100
Subject: [PATCH 181/359] increase resources allocated to FUSIONCATCHER

---
 conf/modules.config | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index 1bdafe99..db8be21a 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -98,6 +98,12 @@ process {
         ]
     }
 
+    witName: FUSIONCATCHER {
+        cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
+        memory = { check_max( 72.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 48.h  * task.attempt, 'time'    ) }
+    }
+
     withName: KALLISTO_INDEX {
         ext.args = '-k 31'
         publishDir = [

From b6ac32fc2485afce199c829b6d9ce19739a7c024 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Mar 2022 10:57:28 +0100
Subject: [PATCH 182/359] update local star copy

---
 modules/local/star/align/meta.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/star/align/meta.yml b/modules/local/star/align/meta.yml
index f34e7ab9..1227c1da 100644
--- a/modules/local/star/align/meta.yml
+++ b/modules/local/star/align/meta.yml
@@ -13,7 +13,7 @@ tools:
       homepage: https://github.com/alexdobin/STAR
       manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
       doi: 10.1093/bioinformatics/bts635
-      licence: ['MIT']
+      licence: ["MIT"]
 input:
   - meta:
       type: map

From eb3c82c1bad334b180dd2833b9a2081e20956937 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Mar 2022 11:17:30 +0100
Subject: [PATCH 183/359] alphabetical ordering of modules.conf

---
 conf/modules.config | 158 +++++++++++++++++++++-----------------------
 1 file changed, 75 insertions(+), 83 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index db8be21a..f961f9b0 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -18,17 +18,23 @@ process {
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
-
-    withName: SAMPLESHEET_CHECK {
+    withName: ARRIBA {
         publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
+            path: { "${params.outdir}/arriba" },
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
+        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv.gz \
+        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
+        ext.prefix =  "${meta.id}.arriba"
     }
 
-    withName: FASTQC {
-        ext.args = '--quiet'
+    withName: ARRIBA_DOWNLOAD {
+        publishDir = [
+            path: { "${params.genomes_base}/arriba" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
     }
 
     withName: CUSTOM_DUMPSOFTWAREVERSIONS {
@@ -47,63 +53,40 @@ process {
         ]
     }
 
-    withName: FUSIONREPORT_DOWNLOAD {
-        publishDir = [
-            path: { "${params.genomes_base}/fusion_report_db" },
-            mode: 'copy',
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
+    withName: FASTQC {
+        ext.args = '--quiet'
     }
 
+    witName: FUSIONCATCHER {
+        cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
+        memory = { check_max( 72.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 48.h  * task.attempt, 'time'    ) }
+    }
 
-    withName: ARRIBA_DOWNLOAD {
+    withName: FUSIONCATCHER_DOWNLOAD {
         publishDir = [
-            path: { "${params.genomes_base}/arriba" },
+            path: { "${params.genomes_base}/fusioncatcher" },
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
 
-    withName: ARRIBA {
+    withName: FUSIONREPORT_DOWNLOAD {
         publishDir = [
-            path: { "${params.outdir}/arriba" },
+            path: { "${params.genomes_base}/fusion_report_db" },
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
-        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv.gz \
-        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
     }
 
-    withName: ERICSCRIPT_DOWNLOAD {
-    publishDir = [
-        path: { "${params.genomes_base}/ericscript" },
-        mode: 'copy',
-        saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
-    ]
-    }
-    withName: STAR_GENOMEGENERATE {
-        ext.args = "--sjdbOverhang ${params.read_length - 1}"
+    withName: GET_PATH {
         publishDir = [
-            path: { "${params.genomes_base}" },
+            path: { "${params.outdir}/collected_fusions" },
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
-    ]
-    }
-
-    withName: FUSIONCATCHER_DOWNLOAD {
-        publishDir = [
-            path: { "${params.genomes_base}/fusioncatcher" },
-            mode: 'copy',
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
 
-    witName: FUSIONCATCHER {
-        cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
-        memory = { check_max( 72.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 48.h  * task.attempt, 'time'    ) }
-    }
-
     withName: KALLISTO_INDEX {
         ext.args = '-k 31'
         publishDir = [
@@ -113,46 +96,29 @@ process {
         ]
     }
 
-    withName: STARFUSION_DOWNLOAD {
-        cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
-        memory = { check_max( 100.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 2.d  * task.attempt, 'time'    ) }
+    withName: PIZZLY {
+        ext.args = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
         publishDir = [
-            path: { "${params.genomes_base}/starfusion" },
+            path: { "${params.outdir}/pizzly" },
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
     }
 
-//TODO document changes from previous rnafusion version to use star-fusion recommendations
-    withName: STAR_FOR_STARFUSION {
+    withName: SAMPLESHEET_CHECK {
         publishDir = [
-            path: { "${params.outdir}/star_for_starfusion" },
+            path: { "${params.outdir}/pipeline_info" },
             mode: 'copy',
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
-        ext.args = '--twopassMode Basic \
-        --outReadsUnmapped None \
-        --readFilesCommand zcat \
-        --outSAMstrandField intronMotif \
-        --outSAMunmapped Within \
-        --chimSegmentMin 12 \
-        --chimJunctionOverhangMin 8 \
-        --chimOutJunctionFormat 1 \
-        --alignSJDBoverhangMin 10 \
-        --alignMatesGapMax 100000 \
-        --alignIntronMax 100000 \
-        --alignSJstitchMismatchNmax 5 -1 5 5 \
-        --chimMultimapScoreRange 3 \
-        --chimScoreJunctionNonGTAG -4 \
-        --chimMultimapNmax 20 \
-        --chimNonchimScoreDropMin 10 \
-        --peOverlapNbasesMin 12 \
-        --peOverlapMMp 0.1 \
-        --alignInsertionFlush Right \
-        --alignSplicedMateMapLminOverLmate 0 \
-        --alignSplicedMateMapLmin 30 \
-        --chimOutType Junctions'
+    }
+
+    withName: SAMTOOLS_SORT {
+        ext.prefix = { "${meta.id}_chimeric_sorted" }
+    }
+
+    withName: SAMTOOLS_VIEW {
+        ext.args = { "-Sb -o ${meta.id}_chimeric.bam" }
     }
 
 //TODO document changes from previous rnafusion version
@@ -197,26 +163,52 @@ process {
         --readFilesCommand zcat'
     }
 
-    withName: PIZZLY {
-        ext.args = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
+//TODO document changes from previous rnafusion version to use star-fusion recommendations
+    withName: STAR_FOR_STARFUSION {
         publishDir = [
-            path: { "${params.outdir}/pizzly" },
+            path: { "${params.outdir}/star_for_starfusion" },
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
+        ext.args = '--twopassMode Basic \
+        --outReadsUnmapped None \
+        --readFilesCommand zcat \
+        --outSAMstrandField intronMotif \
+        --outSAMunmapped Within \
+        --chimSegmentMin 12 \
+        --chimJunctionOverhangMin 8 \
+        --chimOutJunctionFormat 1 \
+        --alignSJDBoverhangMin 10 \
+        --alignMatesGapMax 100000 \
+        --alignIntronMax 100000 \
+        --alignSJstitchMismatchNmax 5 -1 5 5 \
+        --chimMultimapScoreRange 3 \
+        --chimScoreJunctionNonGTAG -4 \
+        --chimMultimapNmax 20 \
+        --chimNonchimScoreDropMin 10 \
+        --peOverlapNbasesMin 12 \
+        --peOverlapMMp 0.1 \
+        --alignInsertionFlush Right \
+        --alignSplicedMateMapLminOverLmate 0 \
+        --alignSplicedMateMapLmin 30 \
+        --chimOutType Junctions'
     }
 
-    withName: SAMTOOLS_VIEW {
-        ext.args = { "-Sb -o ${meta.id}_chimeric.bam" }
-    }
-
-    withName: SAMTOOLS_SORT {
-        ext.prefix = { "${meta.id}_chimeric_sorted" }
+    withName: STAR_GENOMEGENERATE {
+        ext.args = "--sjdbOverhang ${params.read_length - 1}"
+        publishDir = [
+            path: { "${params.genomes_base}" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
     }
 
-    withName: GET_PATH {
+    withName: STARFUSION_DOWNLOAD {
+        cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
+        memory = { check_max( 100.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 2.d  * task.attempt, 'time'    ) }
         publishDir = [
-            path: { "${params.outdir}/collected_fusions" },
+            path: { "${params.genomes_base}/starfusion" },
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]

From 049b05c33fedd1afb49218db77f6e23b50f7e21d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Mar 2022 11:18:07 +0100
Subject: [PATCH 184/359] publish .html report from fusion-report

---
 modules/local/fusionreport/detect/main.nf  | 3 ++-
 modules/local/fusionreport/detect/meta.yml | 5 ++++-
 2 files changed, 6 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 7406255f..06155795 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -19,8 +19,9 @@ process FUSIONREPORT {
     path(fusioncatcher_fusions)
 
     output:
-    path "versions.yml"           , emit: versions
+    path "versions.yml"            , emit: versions
     tuple val(meta), path("*.tsv") , emit: fusion_list
+    tuple val(meta), path(".html") , emit: report
 
     script:
     def tools = params.arriba         ? "--arriba ${arriba_fusions} " : ''
diff --git a/modules/local/fusionreport/detect/meta.yml b/modules/local/fusionreport/detect/meta.yml
index 7767b45b..faefb638 100644
--- a/modules/local/fusionreport/detect/meta.yml
+++ b/modules/local/fusionreport/detect/meta.yml
@@ -46,11 +46,14 @@ output:
         type: file
         description: File containing software versions
         pattern: "versions.yml"
-
   - fusion_list:
         type: file
         description: File containing the summary of all fusions fed-in
         pattern: "*.tsv"
+  - report:
+      type: file
+      description: HTML files
+      pattern: "*.{html}"
 
 authors:
   - "@praveenraj2018, @rannick"

From ca5564c6bc368eee020527040fd107e66f239366 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Mar 2022 11:52:51 +0100
Subject: [PATCH 185/359] small corrections to arriba

---
 conf/modules.config                   | 2 +-
 subworkflows/local/arriba_workflow.nf | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index f961f9b0..696e8c03 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -26,7 +26,7 @@ process {
         ]
         ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv.gz \
         -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
-        ext.prefix =  "${meta.id}.arriba"
+        ext.prefix =  { "${meta.id}.arriba" }
     }
 
     withName: ARRIBA_DOWNLOAD {
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index fd6734da..469e9d59 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -37,7 +37,7 @@ workflow ARRIBA_WORKFLOW {
 
                 GET_PATH_ARRIBA(ARRIBA.out.fusions)
                 ch_arriba_fusions = GET_PATH_ARRIBA.out.file
-                GET_PATH_ARRIBA(ARRIBA.out.fusions_fail)
+                GET_PATH_ARRIBA_FAIL(ARRIBA.out.fusions_fail)
                 ch_arriba_fusion_fail = GET_PATH_ARRIBA_FAIL.out.file
             }
         }

From 0e686f25a48715aa08ff94b6107e4af226a81aec Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Mar 2022 12:12:20 +0100
Subject: [PATCH 186/359] fixes in file handling fusion-report to
 fusioninspector

---
 modules/local/fusioninspector/main.nf     |  6 +++---
 modules/local/fusioninspector/meta.yml    |  4 ++++
 modules/local/fusionreport/detect/main.nf | 14 ++++++++------
 3 files changed, 15 insertions(+), 9 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 941e9bb8..b6a0ac96 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -11,8 +11,8 @@ process FUSIONINSPECTOR {
 
     input:
     tuple val(meta), path(reads)
-    path fusion_list
-    path index
+    tuple val(meta), path(fusion_list)
+    path reference
 
     output:
     path "*"                , emit: output
@@ -24,7 +24,7 @@ process FUSIONINSPECTOR {
     def args = task.ext.args ?: ''
     """
     FusionInspector \\
-        --fusions ${fi_input_list} \\
+        --fusions $fusion_list \\
         --genome_lib ${reference} \\
         $fasta \\
         --CPU ${task.cpus} \\
diff --git a/modules/local/fusioninspector/meta.yml b/modules/local/fusioninspector/meta.yml
index 18459aac..0710b2e9 100644
--- a/modules/local/fusioninspector/meta.yml
+++ b/modules/local/fusioninspector/meta.yml
@@ -28,6 +28,10 @@ input:
 
 # TODO complete output
 output:
+  - versions:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
   - reference:
       type: directory
       description: Genome resource path
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 06155795..ebac5fa4 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -19,9 +19,10 @@ process FUSIONREPORT {
     path(fusioncatcher_fusions)
 
     output:
-    path "versions.yml"            , emit: versions
-    tuple val(meta), path("*.tsv") , emit: fusion_list
-    tuple val(meta), path(".html") , emit: report
+    path "versions.yml"                        , emit: versions
+    tuple val(meta), path("*fusionreport.tsv") , emit: fusion_list
+    tuple val(meta), path("*fusionreport.tsv") , emit: fusion_list_filtered
+    tuple val(meta), path("*.html")            , emit: report
 
     script:
     def tools = params.arriba         ? "--arriba ${arriba_fusions} " : ''
@@ -29,12 +30,13 @@ process FUSIONREPORT {
     tools    += params.squid          ? "--squid ${squid_fusions} " : ''
     tools    += params.starfusion     ? "--starfusion ${starfusion_fusions} " : ''
     tools    += params.fusioncatcher  ? "--fusioncatcher ${fusioncatcher_fusions} " : ''
-
-
-// TODO: mv file to a prefix-including name including
+    def prefix = task.ext.prefix ?: "${meta.id}"
     """
     fusion_report run $meta.id . $fusionreport_ref $tools --allow-multiple-gene-symbols
 
+    mv fusion_list.tsv ${prefix}.fusionreport.tsv
+    mv fusion_list_filtered.tsv ${prefix}.fusionreport_filtered.tsv
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         fusion_report: \$(fusion_report --version)

From c8b63c215d4999bfa070c6d1e45102412dfbd2d9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Mar 2022 16:43:48 +0100
Subject: [PATCH 187/359] address overwriting of fusion-report html files

---
 modules/local/fusionreport/detect/main.nf | 5 +++--
 1 file changed, 3 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index ebac5fa4..f0516bc7 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -22,7 +22,7 @@ process FUSIONREPORT {
     path "versions.yml"                        , emit: versions
     tuple val(meta), path("*fusionreport.tsv") , emit: fusion_list
     tuple val(meta), path("*fusionreport.tsv") , emit: fusion_list_filtered
-    tuple val(meta), path("*.html")            , emit: report
+    tuple val(meta), path("${prefix}/*.html")  , emit: report
 
     script:
     def tools = params.arriba         ? "--arriba ${arriba_fusions} " : ''
@@ -36,7 +36,8 @@ process FUSIONREPORT {
 
     mv fusion_list.tsv ${prefix}.fusionreport.tsv
     mv fusion_list_filtered.tsv ${prefix}.fusionreport_filtered.tsv
-
+    mkdir ${prefix}
+    mv *html ${prefix}/
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         fusion_report: \$(fusion_report --version)

From 4a2c1c0f90a2068cc76210f51eb5005bbb0ec57b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Mar 2022 23:00:16 +0100
Subject: [PATCH 188/359] fix in pizzly

---
 modules/local/pizzly/detect/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index 8453456e..8fef1475 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -27,7 +27,7 @@ process PIZZLY {
         $args \\
         --gtf $gtf \\
         --fasta $transcript \\
-        --output ${prefix}.pizzly ./fusion.txt
+        --output ${prefix}.pizzly $txt
 
     pizzly_flatten_json.py ${prefix}.pizzly.json ${prefix}.pizzly.txt
 

From c4ea401421cb781eafb3e655b69799725ecaae96 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 3 Mar 2022 11:48:22 +0100
Subject: [PATCH 189/359] address overwriting of fusion-report html files, by
 publishing by id

---
 conf/modules.config                        |  8 ++++++++
 modules/local/fusioninspector/meta.yml     |  2 +-
 modules/local/fusionreport/detect/main.nf  | 11 +++++------
 modules/local/fusionreport/detect/meta.yml | 10 +++++-----
 4 files changed, 19 insertions(+), 12 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 696e8c03..91039cd9 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -71,6 +71,14 @@ process {
         ]
     }
 
+    withName: FUSIONREPORT {
+        publishDir = [
+            path: { "${params.outdir}/fusionreport/${meta.id}" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
     withName: FUSIONREPORT_DOWNLOAD {
         publishDir = [
             path: { "${params.genomes_base}/fusion_report_db" },
diff --git a/modules/local/fusioninspector/meta.yml b/modules/local/fusioninspector/meta.yml
index 0710b2e9..5002fe28 100644
--- a/modules/local/fusioninspector/meta.yml
+++ b/modules/local/fusioninspector/meta.yml
@@ -35,7 +35,7 @@ output:
   - reference:
       type: directory
       description: Genome resource path
-      pattern: "star-fusion-genome"
+      pattern: "*"
 
 authors:
   - "@rannick"
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index f0516bc7..adff8796 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -19,10 +19,10 @@ process FUSIONREPORT {
     path(fusioncatcher_fusions)
 
     output:
-    path "versions.yml"                        , emit: versions
-    tuple val(meta), path("*fusionreport.tsv") , emit: fusion_list
-    tuple val(meta), path("*fusionreport.tsv") , emit: fusion_list_filtered
-    tuple val(meta), path("${prefix}/*.html")  , emit: report
+    path "versions.yml"                                  , emit: versions
+    tuple val(meta), path("*fusionreport.tsv")           , emit: fusion_list
+    tuple val(meta), path("*fusionreport_filtered.tsv")  , emit: fusion_list_filtered
+    tuple val(meta), path("*.html")                      , emit: report
 
     script:
     def tools = params.arriba         ? "--arriba ${arriba_fusions} " : ''
@@ -36,8 +36,7 @@ process FUSIONREPORT {
 
     mv fusion_list.tsv ${prefix}.fusionreport.tsv
     mv fusion_list_filtered.tsv ${prefix}.fusionreport_filtered.tsv
-    mkdir ${prefix}
-    mv *html ${prefix}/
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         fusion_report: \$(fusion_report --version)
diff --git a/modules/local/fusionreport/detect/meta.yml b/modules/local/fusionreport/detect/meta.yml
index faefb638..72101495 100644
--- a/modules/local/fusionreport/detect/meta.yml
+++ b/modules/local/fusionreport/detect/meta.yml
@@ -26,16 +26,16 @@ input:
       pattern: "*.fusions.tsv"
   - pizzly_fusions:
       type: path
-      description: File containing fusions from arriba
-      pattern: "*.fusions.tsv"
+      description: File containing fusions from pizzly
+      pattern: "*.pizzly.txt"
   - squid_fusions:
       type: path
       description: File containing fusions from squid
-      pattern: "*.fusions.tsv"
+      pattern: "*.annotated.txt"
   - starfusion_fusions:
       type: path
       description: File containing fusions from STARfusion
-      pattern: "*.fusions.tsv"
+      pattern: "*.starfusion.fusion_predictions.tsv"
   - fusioncatcher_fusions:
       type: path
       description: File containing fusions from fusioncatcher
@@ -53,7 +53,7 @@ output:
   - report:
       type: file
       description: HTML files
-      pattern: "*.{html}"
+      pattern: "*.html"
 
 authors:
   - "@praveenraj2018, @rannick"

From 63769511bfecfe07eee9350af415b44b376c71bc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 3 Mar 2022 16:25:03 +0100
Subject: [PATCH 190/359] update fusion-report container to have the correct
 regular expression parser for COSMIC

---
 modules/local/fusionreport/detect/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index adff8796..6647065b 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -5,8 +5,8 @@ process FUSIONREPORT {
     // Note: 2.7X indices incompatible with AWS iGenomes.
     conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'docker.io/rannickscilifelab/fusion-report:2.1.5' :
-        'docker.io/rannickscilifelab/fusion-report:2.1.5' }"
+        'docker.io/rannickscilifelab/fusion-report:2.1.5a' :
+        'docker.io/rannickscilifelab/fusion-report:2.1.5a' }"
 
 
     input:

From 929690bf175d109b6baa1091c5cec162058130de Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Mar 2022 11:30:11 +0100
Subject: [PATCH 191/359] update to fusion-report containter that only outputs
 reports for filtered fusions

---
 modules/local/fusionreport/detect/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 6647065b..497d614f 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -5,8 +5,8 @@ process FUSIONREPORT {
     // Note: 2.7X indices incompatible with AWS iGenomes.
     conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'docker.io/rannickscilifelab/fusion-report:2.1.5a' :
-        'docker.io/rannickscilifelab/fusion-report:2.1.5a' }"
+        'docker.io/rannickscilifelab/fusion-report:2.1.5b' :
+        'docker.io/rannickscilifelab/fusion-report:2.1.5b' }"
 
 
     input:

From d5560b0436fa0e5500018da20846644ec61f8b54 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Mar 2022 17:07:15 +0100
Subject: [PATCH 192/359] update fusion-report docker container to include
 updated MitelmanDB and FusionGDB2 on top of FusionGDB

---
 modules/local/fusionreport/detect/main.nf   | 4 ++--
 modules/local/fusionreport/download/main.nf | 4 ++--
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 497d614f..625316b9 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -5,8 +5,8 @@ process FUSIONREPORT {
     // Note: 2.7X indices incompatible with AWS iGenomes.
     conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'docker.io/rannickscilifelab/fusion-report:2.1.5b' :
-        'docker.io/rannickscilifelab/fusion-report:2.1.5b' }"
+        'docker.io/rannickscilifelab/fusion-report:2.1.5updated' :
+        'docker.io/rannickscilifelab/fusion-report:2.1.5updated' }"
 
 
     input:
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index f0e1e57e..ae51f880 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -5,8 +5,8 @@ process FUSIONREPORT_DOWNLOAD {
     // Note: 2.7X indices incompatible with AWS iGenomes.
     conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'docker.io/rannickscilifelab/fusion-report:2.1.5' :
-        'docker.io/rannickscilifelab/fusion-report:2.1.5' }"
+        'docker.io/rannickscilifelab/fusion-report:2.1.5updated' :
+        'docker.io/rannickscilifelab/fusion-report:2.1.5updated' }"
 
 
     input:

From c84fe1421ea6f03cd23c8a17a79eef36c0814ed9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 9 Mar 2022 10:39:55 +0100
Subject: [PATCH 193/359] add arriba visualisation

---
 conf/modules.config                           | 28 +++++++++-
 modules.json                                  |  3 ++
 modules/local/arriba/visualisation/main.nf    | 41 ++++++++++++++
 modules/local/arriba/visualisation/meta.yml   | 54 +++++++++++++++++++
 .../nf-core/modules/samtools/index/main.nf    | 36 +++++++++++++
 .../nf-core/modules/samtools/index/meta.yml   | 53 ++++++++++++++++++
 subworkflows/local/arriba_workflow.nf         | 49 +++++++++++------
 subworkflows/local/squid_workflow.nf          | 17 +++---
 8 files changed, 255 insertions(+), 26 deletions(-)
 create mode 100644 modules/local/arriba/visualisation/main.nf
 create mode 100644 modules/local/arriba/visualisation/meta.yml
 create mode 100644 modules/nf-core/modules/samtools/index/main.nf
 create mode 100644 modules/nf-core/modules/samtools/index/meta.yml

diff --git a/conf/modules.config b/conf/modules.config
index 91039cd9..65ce1b06 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -37,6 +37,16 @@ process {
         ]
     }
 
+        withName: ARRIBA_VISUALISATION {
+        publishDir = [
+            path: { "${params.outdir}/arriba_visualisation" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+        ext.args = "cytobands_hg38_GRCh38_v2.1.0.tsv"
+        ext.args2 = "protein_domains_hg38_GRCh38_v2.1.0.gff3"
+    }
+
     withName: CUSTOM_DUMPSOFTWAREVERSIONS {
         publishDir = [
             path: { "${params.outdir}/pipeline_info" },
@@ -121,11 +131,25 @@ process {
         ]
     }
 
-    withName: SAMTOOLS_SORT {
+    withName: SAMTOOLS_SORT_FOR_ARRIBA {
+        ext.prefix = { "${meta.id}_sorted" }
+        publishDir = [
+            path: { "${params.outdir}/samtools_sort_for_arriba" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
+    withName: SAMTOOLS_SORT_FOR_SQUID {
         ext.prefix = { "${meta.id}_chimeric_sorted" }
+        publishDir = [
+            path: { "${params.outdir}/samtools_sort_for_squid" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
     }
 
-    withName: SAMTOOLS_VIEW {
+    withName: SAMTOOLS_VIEW_FOR_SQUID {
         ext.args = { "-Sb -o ${meta.id}_chimeric.bam" }
     }
 
diff --git a/modules.json b/modules.json
index be505243..b7ee22d9 100644
--- a/modules.json
+++ b/modules.json
@@ -24,6 +24,9 @@
             "samtools/faidx": {
                 "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
             },
+            "samtools/index": {
+                "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
+            },
             "samtools/sort": {
                 "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
             },
diff --git a/modules/local/arriba/visualisation/main.nf b/modules/local/arriba/visualisation/main.nf
new file mode 100644
index 00000000..7dbbe23b
--- /dev/null
+++ b/modules/local/arriba/visualisation/main.nf
@@ -0,0 +1,41 @@
+process ARRIBA_VISUALISATION {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::arriba=2.1.0" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/arriba:2.1.0--h3198e80_1' :
+        'quay.io/biocontainers/arriba:2.1.0--h3198e80_1' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+    path fusions
+    path reference
+    path gtf
+
+    output:
+    tuple val(meta), path("*.pdf")          , emit: pdf
+    path "versions.yml"                     , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    draw_fusions.R \\
+        --fusions=$fusions \\
+        --alignments=$bam \\
+        --output=${prefix}.pdf \\
+        --annotation=${gtf} \\
+        --cytobands=${reference}/${args} \\
+        --proteinDomains=${reference}/${args2}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        arriba: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/arriba/visualisation/meta.yml b/modules/local/arriba/visualisation/meta.yml
new file mode 100644
index 00000000..4bde2f08
--- /dev/null
+++ b/modules/local/arriba/visualisation/meta.yml
@@ -0,0 +1,54 @@
+name: arriba
+description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
+keywords:
+  - fusion
+  - arriba
+tools:
+  - arriba:
+      description: Fast and accurate gene fusion detection from RNA-Seq data
+      homepage: https://github.com/suhrig/arriba
+      documentation: https://arriba.readthedocs.io/en/latest/
+      tool_dev_url: https://github.com/suhrig/arriba
+      doi: "10.1101/gr.257246.119"
+      licence: ["MIT"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - fasta:
+      type: file
+      description: Assembly FASTA file
+      pattern: "*.{fasta}"
+  - gtf:
+      type: file
+      description: Annotation GTF file
+      pattern: "*.{gtf}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - fusions:
+      type: file
+      description: File contains fusions which pass all of Arriba's filters.
+      pattern: "*.{fusions.tsv}"
+  - fusions_fail:
+      type: file
+      description: File contains fusions that Arriba classified as an artifact or that are also observed in healthy tissue.
+      pattern: "*.{fusions.discarded.tsv}"
+
+authors:
+  - "@praveenraj2018"
diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf
new file mode 100644
index 00000000..e41cdcc8
--- /dev/null
+++ b/modules/nf-core/modules/samtools/index/main.nf
@@ -0,0 +1,36 @@
+process SAMTOOLS_INDEX {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda (params.enable_conda ? "bioconda::samtools=1.15" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' :
+        'quay.io/biocontainers/samtools:1.15--h1170115_1' }"
+
+    input:
+    tuple val(meta), path(input)
+
+    output:
+    tuple val(meta), path("*.bai") , optional:true, emit: bai
+    tuple val(meta), path("*.csi") , optional:true, emit: csi
+    tuple val(meta), path("*.crai"), optional:true, emit: crai
+    path  "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    samtools \\
+        index \\
+        -@ ${task.cpus-1} \\
+        $args \\
+        $input
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/samtools/index/meta.yml b/modules/nf-core/modules/samtools/index/meta.yml
new file mode 100644
index 00000000..e5cadbc2
--- /dev/null
+++ b/modules/nf-core/modules/samtools/index/meta.yml
@@ -0,0 +1,53 @@
+name: samtools_index
+description: Index SAM/BAM/CRAM file
+keywords:
+  - index
+  - bam
+  - sam
+  - cram
+tools:
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: hhttp://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bai:
+      type: file
+      description: BAM/CRAM/SAM index file
+      pattern: "*.{bai,crai,sai}"
+  - crai:
+      type: file
+      description: BAM/CRAM/SAM index file
+      pattern: "*.{bai,crai,sai}"
+  - csi:
+      type: file
+      description: CSI index file
+      pattern: "*.{csi}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
+  - "@ewels"
+  - "@maxulysse"
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 469e9d59..16b52419 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -2,10 +2,13 @@
 // Check input samplesheet and get read channels
 //
 
-include { STAR_ALIGN as STAR_FOR_ARRIBA }         from '../../modules/nf-core/modules/star/align/main'
-include { ARRIBA }                                from '../../modules/nf-core/modules/arriba/main'
-include { GET_PATH as GET_PATH_ARRIBA }           from '../../modules/local/getpath/main'
-include { GET_PATH as GET_PATH_ARRIBA_FAIL }      from '../../modules/local/getpath/main'
+include { ARRIBA }                                      from '../../modules/nf-core/modules/arriba/main'
+include { ARRIBA_VISUALISATION }                        from '../../modules/local/arriba/visualisation/main'
+include { GET_PATH as GET_PATH_ARRIBA }                 from '../../modules/local/getpath/main'
+include { GET_PATH as GET_PATH_ARRIBA_FAIL }            from '../../modules/local/getpath/main'
+include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_ARRIBA }   from '../../modules/nf-core/modules/samtools/sort/main'
+include { SAMTOOLS_INDEX }                              from '../../modules/nf-core/modules/samtools/index/main'
+include { STAR_ALIGN as STAR_FOR_ARRIBA }               from '../../modules/nf-core/modules/star/align/main'
 
 
 workflow ARRIBA_WORKFLOW {
@@ -19,36 +22,52 @@ workflow ARRIBA_WORKFLOW {
         ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
         if (params.arriba) {
+            gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+            star_ignore_sjdbgtf = false
+            seq_platform = false
+            seq_center = false
+
+            STAR_FOR_ARRIBA( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+            ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
+
+            SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam)
+            ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_ARRIBA.out.versions)
+
+            SAMTOOLS_INDEX(SAMTOOLS_SORT_FOR_ARRIBA.out.bam)
+            ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions)
+
+            bam_indexed = SAMTOOLS_SORT_FOR_ARRIBA.out.bam.join(SAMTOOLS_INDEX.out.bai)
+
             if (params.arriba_fusions) {
                 ch_arriba_fusions = params.arriba_fusions
                 ch_arriba_fusion_fail = ch_dummy_file
             } else {
-                gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-                star_ignore_sjdbgtf = false
-                seq_platform = false
-                seq_center = false
-
-                STAR_FOR_ARRIBA( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
-                ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
-
-
                 ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
                 ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
                 GET_PATH_ARRIBA(ARRIBA.out.fusions)
                 ch_arriba_fusions = GET_PATH_ARRIBA.out.file
+
                 GET_PATH_ARRIBA_FAIL(ARRIBA.out.fusions_fail)
                 ch_arriba_fusion_fail = GET_PATH_ARRIBA_FAIL.out.file
             }
+
+            ARRIBA_VISUALISATION(bam_indexed, ch_arriba_fusions, params.arriba_ref, gtf)
+            ch_versions = ch_versions.mix(ARRIBA_VISUALISATION.out.versions)
+
+            ch_arriba_visualisation = ARRIBA_VISUALISATION.out.pdf
+
         }
         else {
-            ch_arriba_fusions = ch_dummy_file
-            ch_arriba_fusion_fail = ch_dummy_file
+            ch_arriba_fusions       = ch_dummy_file
+            ch_arriba_fusion_fail   = ch_dummy_file
+            ch_arriba_visualisation = ch_dummy_file
         }
 
     emit:
         fusions         = ch_arriba_fusions
         fusions_fail    = ch_arriba_fusion_fail
         versions        = ch_versions.ifEmpty(null)
+        pdf             = ch_arriba_visualisation
     }
 
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 6e3aee8f..6814c418 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -2,13 +2,12 @@
 // Check input samplesheet and get read channels
 //
 
-include { STAR_ALIGN as STAR_FOR_SQUID }    from '../../modules/local/star/align/main'
-include { SAMTOOLS_VIEW }                   from '../../modules/nf-core/modules/samtools/view/main'
-include { SAMTOOLS_SORT }                   from '../../modules/nf-core/modules/samtools/sort/main'
-include { SQUID }                           from '../../modules/local/squid/detect/main'
-include { SQUID_ANNOTATE }                  from '../../modules/local/squid/annotate/main'
-include { GET_PATH }                         from '../../modules/local/getpath/main'
-
+include { GET_PATH }                                    from '../../modules/local/getpath/main'
+include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_SQUID }    from '../../modules/nf-core/modules/samtools/sort/main'
+include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_SQUID }    from '../../modules/nf-core/modules/samtools/view/main'
+include { SQUID }                                       from '../../modules/local/squid/detect/main'
+include { SQUID_ANNOTATE }                              from '../../modules/local/squid/annotate/main'
+include { STAR_ALIGN as STAR_FOR_SQUID }                from '../../modules/local/star/align/main'
 
 workflow SQUID_WORKFLOW {
     take:
@@ -32,10 +31,10 @@ workflow SQUID_WORKFLOW {
             STAR_FOR_SQUID( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
-            SAMTOOLS_VIEW ( STAR_FOR_SQUID.out.sam, [] )
+            SAMTOOLS_VIEW_FOR_SQUID ( STAR_FOR_SQUID.out.sam, [] )
             ch_versions = ch_versions.mix(SAMTOOLS_VIEW.out.versions )
 
-            SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam )
+            SAMTOOLS_SORT_FOR_SQUID ( SAMTOOLS_VIEW.out.bam )
             ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions )
 
             bam_sorted = STAR_FOR_SQUID.out.bam_sorted.join(SAMTOOLS_SORT.out.bam )

From 53da739fe2da2280dd02cd27ba6a76821f43761b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 9 Mar 2022 11:00:28 +0100
Subject: [PATCH 194/359] update arriba visualisation meta

---
 modules/local/arriba/visualisation/meta.yml | 26 ++++++++++-----------
 1 file changed, 13 insertions(+), 13 deletions(-)

diff --git a/modules/local/arriba/visualisation/meta.yml b/modules/local/arriba/visualisation/meta.yml
index 4bde2f08..a7418ca2 100644
--- a/modules/local/arriba/visualisation/meta.yml
+++ b/modules/local/arriba/visualisation/meta.yml
@@ -1,7 +1,7 @@
-name: arriba
+name: arriba_visualisation
 description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
 keywords:
-  - fusion
+  - visualisation
   - arriba
 tools:
   - arriba:
@@ -22,10 +22,14 @@ input:
       type: file
       description: BAM/CRAM/SAM file
       pattern: "*.{bam,cram,sam}"
-  - fasta:
+  - bai:
       type: file
-      description: Assembly FASTA file
-      pattern: "*.{fasta}"
+      description: BAMindex file
+      pattern: "*.{bai}"
+  - fusions:
+      type: file
+      description: Arriba fusions file
+      pattern: "*.{tsv}"
   - gtf:
       type: file
       description: Annotation GTF file
@@ -41,14 +45,10 @@ output:
       type: file
       description: File containing software versions
       pattern: "versions.yml"
-  - fusions:
-      type: file
-      description: File contains fusions which pass all of Arriba's filters.
-      pattern: "*.{fusions.tsv}"
-  - fusions_fail:
+  - pdf:
       type: file
-      description: File contains fusions that Arriba classified as an artifact or that are also observed in healthy tissue.
-      pattern: "*.{fusions.discarded.tsv}"
+      description: File contains fusions visualisation
+      pattern: "*.{pdf}"
 
 authors:
-  - "@praveenraj2018"
+  - "@rannick"

From 9b92b0a4b75b8c5bc265204dd40e3241f012a4ab Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Wed, 9 Mar 2022 13:50:03 +0100
Subject: [PATCH 195/359] Tiny bugfix for SAMTOOLS_VIEW_FOR_SQUID

---
 subworkflows/local/squid_workflow.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 6814c418..aa32261c 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -32,9 +32,9 @@ workflow SQUID_WORKFLOW {
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
             SAMTOOLS_VIEW_FOR_SQUID ( STAR_FOR_SQUID.out.sam, [] )
-            ch_versions = ch_versions.mix(SAMTOOLS_VIEW.out.versions )
+            ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_SQUID.out.versions )
 
-            SAMTOOLS_SORT_FOR_SQUID ( SAMTOOLS_VIEW.out.bam )
+            SAMTOOLS_SORT_FOR_SQUID ( SAMTOOLS_VIEW_FOR_SQUID.out.bam )
             ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions )
 
             bam_sorted = STAR_FOR_SQUID.out.bam_sorted.join(SAMTOOLS_SORT.out.bam )

From f03e5c6f5b6a1c8b6a4d3bc955df26f40e887258 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Wed, 9 Mar 2022 13:50:28 +0100
Subject: [PATCH 196/359] And for SAMTOOLS_SORT

---
 subworkflows/local/squid_workflow.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index aa32261c..be2a485f 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -35,9 +35,9 @@ workflow SQUID_WORKFLOW {
             ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_SQUID.out.versions )
 
             SAMTOOLS_SORT_FOR_SQUID ( SAMTOOLS_VIEW_FOR_SQUID.out.bam )
-            ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions )
+            ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_SQUID.out.versions )
 
-            bam_sorted = STAR_FOR_SQUID.out.bam_sorted.join(SAMTOOLS_SORT.out.bam )
+            bam_sorted = STAR_FOR_SQUID.out.bam_sorted.join(SAMTOOLS_SORT_FOR_SQUID.out.bam )
 
             SQUID ( bam_sorted )
             ch_versions = ch_versions.mix(SQUID.out.versions)

From 54057c76cb422494fea2209c950b44540bd50862 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 15 Mar 2022 20:58:33 +0000
Subject: [PATCH 197/359] Template update for nf-core/tools version 2.3

---
 .github/ISSUE_TEMPLATE/bug_report.yml        |   1 -
 .github/PULL_REQUEST_TEMPLATE.md             |   2 +-
 .github/workflows/awsfulltest.yml            |   6 +-
 .github/workflows/awstest.yml                |   6 +-
 .github/workflows/ci.yml                     |   4 +-
 .github/workflows/linting.yml                |  28 +-
 .gitpod.yml                                  |  14 +
 .nf-core.yml                                 |   1 +
 .yamllint.yml                                |   6 +
 LICENSE                                      |   2 +-
 README.md                                    |   8 +-
 assets/nf-core-rnafusion_logo_light.png      | Bin 288 -> 10989 bytes
 bin/check_samplesheet.py                     | 346 ++++++++++++-------
 conf/base.config                             |   4 +-
 conf/igenomes.config                         |  80 ++---
 conf/modules.config                          |  18 +-
 conf/test.config                             |   6 +-
 conf/test_full.config                        |   6 +-
 docs/images/nf-core-rnafusion_logo_dark.png  | Bin 288 -> 72761 bytes
 docs/images/nf-core-rnafusion_logo_light.png | Bin 288 -> 72629 bytes
 docs/usage.md                                |  21 +-
 lib/NfcoreSchema.groovy                      |   4 +-
 lib/Utils.groovy                             |   4 +-
 lib/WorkflowRnafusion.groovy                 |   4 +-
 main.nf                                      |  24 +-
 nextflow.config                              |  20 +-
 nextflow_schema.json                         |  23 +-
 subworkflows/local/input_check.nf            |  18 +-
 workflows/rnafusion.nf                       |  28 +-
 29 files changed, 422 insertions(+), 262 deletions(-)
 create mode 100644 .gitpod.yml
 create mode 100644 .nf-core.yml
 create mode 100644 .yamllint.yml

diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index a3de1c24..ca9d2123 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -1,4 +1,3 @@
-
 name: Bug report
 description: Report something that is broken or incorrect
 labels: bug
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 026428de..6bf2080a 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -19,7 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
     - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
     - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint`).
-- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
+- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker` --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
 - [ ] `CHANGELOG.md` is updated.
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 64316f92..ad566b83 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -14,7 +14,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@v2
+        uses: nf-core/tower-action@v3
         # TODO nf-core: You can customise AWS full pipeline tests as required
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
@@ -31,4 +31,6 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
             }
           profiles: test_full,aws_tower
-          pre_run_script: 'export NXF_VER=21.10.3'
+          nextflow_config: |
+            process.errorStrategy = 'retry'
+            process.maxRetries = 3
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 2c933fcc..ae430044 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@v2
+        uses: nf-core/tower-action@v3
         
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
@@ -25,4 +25,6 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-test-${{ github.sha }}"
             }
           profiles: test,aws_tower
-          pre_run_script: 'export NXF_VER=21.10.3'
+          nextflow_config: |
+            process.errorStrategy = 'retry'
+            process.maxRetries = 3
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index f9ff5247..2eef1cc9 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -14,7 +14,7 @@ env:
 
 jobs:
   test:
-    name: Run workflow tests
+    name: Run pipeline with test data
     # Only run on push if this is the nf-core dev branch (merged PRs)
     if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}
     runs-on: ubuntu-latest
@@ -47,4 +47,4 @@ jobs:
         # For example: adding multiple test runs with different parameters
         # Remember that you can parallelise this by using strategy.matrix
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,docker
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 3b448773..fda934c0 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -12,9 +12,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - uses: actions/checkout@v2
-      - uses: actions/setup-node@v1
-        with:
-          node-version: '10'
+      - uses: actions/setup-node@v2
       - name: Install markdownlint
         run: npm install -g markdownlint-cli
       - name: Run Markdownlint
@@ -51,9 +49,7 @@ jobs:
     steps:
       - uses: actions/checkout@v2
 
-      - uses: actions/setup-node@v1
-        with:
-          node-version: '10'
+      - uses: actions/setup-node@v2
 
       - name: Install editorconfig-checker
         run: npm install -g editorconfig-checker
@@ -64,14 +60,13 @@ jobs:
   YAML:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v1
-      - uses: actions/setup-node@v1
+      - name: Checkout
+        uses: actions/checkout@master
+      - name: 'Yamllint'
+        uses: karancode/yamllint-github-action@master
         with:
-          node-version: '10'
-      - name: Install yaml-lint
-        run: npm install -g yaml-lint
-      - name: Run yaml-lint
-        run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml" -o -name "*.yaml")
+          yamllint_file_or_dir: '.'
+          yamllint_config_filepath: '.yamllint.yml'
 
       # If the above check failed, post a comment on the PR explaining the failure
       - name: Post PR comment
@@ -84,10 +79,11 @@ jobs:
             To keep the code consistent with lots of contributors, we run automated code consistency checks.
             To fix this CI test, please run:
 
-            * Install `yaml-lint`
-                * [Install `npm`](https://www.npmjs.com/get-npm) then [install `yaml-lint`](https://www.npmjs.com/package/yaml-lint) (`npm install -g yaml-lint`)
+            * Install `yamllint`
+                * Install `yamllint` following [this](https://yamllint.readthedocs.io/en/stable/quickstart.html#installing-yamllint)
+                instructions or alternative install it in your [conda environment](https://anaconda.org/conda-forge/yamllint)
             * Fix the markdown errors
-                * Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml")`
+                * Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml") -c ./.yamllint.yml`
                 * Fix any reported errors in your YAML files
 
             Once you push these changes the test should pass, and you can hide this comment :+1:
diff --git a/.gitpod.yml b/.gitpod.yml
new file mode 100644
index 00000000..b7d4cee1
--- /dev/null
+++ b/.gitpod.yml
@@ -0,0 +1,14 @@
+image: nfcore/gitpod:latest
+
+vscode:
+  extensions: # based on nf-core.nf-core-extensionpack
+    - codezombiech.gitignore                 # Language support for .gitignore files
+    # - cssho.vscode-svgviewer                 # SVG viewer
+    - davidanson.vscode-markdownlint         # Markdown/CommonMark linting and style checking for Visual Studio Code
+    - eamodio.gitlens                        # Quickly glimpse into whom, why, and when a line or code block was changed
+    - EditorConfig.EditorConfig              # override user/workspace settings with settings found in .editorconfig files
+    - Gruntfuggly.todo-tree                  # Display TODO and FIXME in a tree view in the activity bar
+    - mechatroner.rainbow-csv                # Highlight columns in csv files in different colors
+    # - nextflow.nextflow                      # Nextflow syntax highlighting
+    - oderwat.indent-rainbow                 # Highlight indentation level
+    - streetsidesoftware.code-spell-checker  # Spelling checker for source code
diff --git a/.nf-core.yml b/.nf-core.yml
new file mode 100644
index 00000000..3805dc81
--- /dev/null
+++ b/.nf-core.yml
@@ -0,0 +1 @@
+repository_type: pipeline
diff --git a/.yamllint.yml b/.yamllint.yml
new file mode 100644
index 00000000..d466deec
--- /dev/null
+++ b/.yamllint.yml
@@ -0,0 +1,6 @@
+extends: default
+
+rules:
+    document-start: disable
+    line-length: disable
+    truthy: disable
diff --git a/LICENSE b/LICENSE
index ac8adf18..8a50c49d 100644
--- a/LICENSE
+++ b/LICENSE
@@ -1,6 +1,6 @@
 MIT License
 
-Copyright (c) Martin Proks
+Copyright (c) Martin Proks, @praveenraj2018
 
 Permission is hereby granted, free of charge, to any person obtaining a copy
 of this software and associated documentation files (the "Software"), to deal
diff --git a/README.md b/README.md
index 23fffa60..5beef0b2 100644
--- a/README.md
+++ b/README.md
@@ -40,14 +40,14 @@ On release, automated continuous integration tests run the pipeline on a full-si
 3. Download the pipeline and test it on a minimal dataset with a single command:
 
     ```console
-    nextflow run nf-core/rnafusion -profile test,YOURPROFILE
+    nextflow run nf-core/rnafusion -profile test,YOURPROFILE --outdir <OUTDIR>
     ```
 
     Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
 
     > * The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
     > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
-    > * If you are using `singularity` and are persistently observing issues downloading Singularity images directly due to timeout or network issues, then you can use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, you can use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
+    > * If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
     > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
 
 4. Start running your own analysis!
@@ -55,7 +55,7 @@ On release, automated continuous integration tests run the pipeline on a full-si
     <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
 
     ```console
-    nextflow run nf-core/rnafusion -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input samplesheet.csv --genome GRCh37
+    nextflow run nf-core/rnafusion --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
     ```
 
 ## Documentation
@@ -64,7 +64,7 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline [usag
 
 ## Credits
 
-nf-core/rnafusion was originally written by Martin Proks.
+nf-core/rnafusion was originally written by Martin Proks, @praveenraj2018.
 
 We thank the following people for their extensive assistance in the development of this pipeline:
 
diff --git a/assets/nf-core-rnafusion_logo_light.png b/assets/nf-core-rnafusion_logo_light.png
index dee3db82069b61e9a5380d940355f47ff565057c..55f385411694bc192b1d6cf172a0c7defcd49d1a 100644
GIT binary patch
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diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index dff05443..5473b624 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -1,145 +1,249 @@
 #!/usr/bin/env python
 
-# TODO nf-core: Update the script to check the samplesheet
-# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
 
-import os
-import sys
-import errno
+"""Provide a command line tool to validate and transform tabular samplesheets."""
+
+
 import argparse
+import csv
+import logging
+import sys
+from collections import Counter
+from pathlib import Path
 
 
-def parse_args(args=None):
-    Description = "Reformat nf-core/rnafusion samplesheet file and check its contents."
-    Epilog = "Example usage: python check_samplesheet.py <FILE_IN> <FILE_OUT>"
+logger = logging.getLogger()
 
-    parser = argparse.ArgumentParser(description=Description, epilog=Epilog)
-    parser.add_argument("FILE_IN", help="Input samplesheet file.")
-    parser.add_argument("FILE_OUT", help="Output file.")
-    return parser.parse_args(args)
 
+class RowChecker:
+    """
+    Define a service that can validate and transform each given row.
 
-def make_dir(path):
-    if len(path) > 0:
-        try:
-            os.makedirs(path)
-        except OSError as exception:
-            if exception.errno != errno.EEXIST:
-                raise exception
+    Attributes:
+        modified (list): A list of dicts, where each dict corresponds to a previously
+            validated and transformed row. The order of rows is maintained.
 
+    """
 
-def print_error(error, context="Line", context_str=""):
-    error_str = "ERROR: Please check samplesheet -> {}".format(error)
-    if context != "" and context_str != "":
-        error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format(
-            error, context.strip(), context_str.strip()
+    VALID_FORMATS = (
+        ".fq.gz",
+        ".fastq.gz",
+    )
+
+    def __init__(
+        self,
+        sample_col="sample",
+        first_col="fastq_1",
+        second_col="fastq_2",
+        single_col="single_end",
+        **kwargs,
+    ):
+        """
+        Initialize the row checker with the expected column names.
+
+        Args:
+            sample_col (str): The name of the column that contains the sample name
+                (default "sample").
+            first_col (str): The name of the column that contains the first (or only)
+                FASTQ file path (default "fastq_1").
+            second_col (str): The name of the column that contains the second (if any)
+                FASTQ file path (default "fastq_2").
+            single_col (str): The name of the new column that will be inserted and
+                records whether the sample contains single- or paired-end sequencing
+                reads (default "single_end").
+
+        """
+        super().__init__(**kwargs)
+        self._sample_col = sample_col
+        self._first_col = first_col
+        self._second_col = second_col
+        self._single_col = single_col
+        self._seen = set()
+        self.modified = []
+
+    def validate_and_transform(self, row):
+        """
+        Perform all validations on the given row and insert the read pairing status.
+
+        Args:
+            row (dict): A mapping from column headers (keys) to elements of that row
+                (values).
+
+        """
+        self._validate_sample(row)
+        self._validate_first(row)
+        self._validate_second(row)
+        self._validate_pair(row)
+        self._seen.add((row[self._sample_col], row[self._first_col]))
+        self.modified.append(row)
+
+    def _validate_sample(self, row):
+        """Assert that the sample name exists and convert spaces to underscores."""
+        assert len(row[self._sample_col]) > 0, "Sample input is required."
+        # Sanitize samples slightly.
+        row[self._sample_col] = row[self._sample_col].replace(" ", "_")
+
+    def _validate_first(self, row):
+        """Assert that the first FASTQ entry is non-empty and has the right format."""
+        assert len(row[self._first_col]) > 0, "At least the first FASTQ file is required."
+        self._validate_fastq_format(row[self._first_col])
+
+    def _validate_second(self, row):
+        """Assert that the second FASTQ entry has the right format if it exists."""
+        if len(row[self._second_col]) > 0:
+            self._validate_fastq_format(row[self._second_col])
+
+    def _validate_pair(self, row):
+        """Assert that read pairs have the same file extension. Report pair status."""
+        if row[self._first_col] and row[self._second_col]:
+            row[self._single_col] = False
+            assert (
+                Path(row[self._first_col]).suffixes == Path(row[self._second_col]).suffixes
+            ), "FASTQ pairs must have the same file extensions."
+        else:
+            row[self._single_col] = True
+
+    def _validate_fastq_format(self, filename):
+        """Assert that a given filename has one of the expected FASTQ extensions."""
+        assert any(filename.endswith(extension) for extension in self.VALID_FORMATS), (
+            f"The FASTQ file has an unrecognized extension: {filename}\n"
+            f"It should be one of: {', '.join(self.VALID_FORMATS)}"
         )
-    print(error_str)
-    sys.exit(1)
 
+    def validate_unique_samples(self):
+        """
+        Assert that the combination of sample name and FASTQ filename is unique.
+
+        In addition to the validation, also rename the sample if more than one sample,
+        FASTQ file combination exists.
+
+        """
+        assert len(self._seen) == len(self.modified), "The pair of sample name and FASTQ must be unique."
+        if len({pair[0] for pair in self._seen}) < len(self._seen):
+            counts = Counter(pair[0] for pair in self._seen)
+            seen = Counter()
+            for row in self.modified:
+                sample = row[self._sample_col]
+                seen[sample] += 1
+                if counts[sample] > 1:
+                    row[self._sample_col] = f"{sample}_T{seen[sample]}"
+
+
+def sniff_format(handle):
+    """
+    Detect the tabular format.
+
+    Args:
+        handle (text file): A handle to a `text file`_ object. The read position is
+        expected to be at the beginning (index 0).
+
+    Returns:
+        csv.Dialect: The detected tabular format.
+
+    .. _text file:
+        https://docs.python.org/3/glossary.html#term-text-file
 
-# TODO nf-core: Update the check_samplesheet function
-def check_samplesheet(file_in, file_out):
     """
-    This function checks that the samplesheet follows the following structure:
+    peek = handle.read(2048)
+    sniffer = csv.Sniffer()
+    if not sniffer.has_header(peek):
+        logger.critical(f"The given sample sheet does not appear to contain a header.")
+        sys.exit(1)
+    dialect = sniffer.sniff(peek)
+    handle.seek(0)
+    return dialect
 
-    sample,fastq_1,fastq_2
-    SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz
-    SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz
-    SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,
 
-    For an example see:
-    https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
+def check_samplesheet(file_in, file_out):
     """
+    Check that the tabular samplesheet has the structure expected by nf-core pipelines.
 
-    sample_mapping_dict = {}
-    with open(file_in, "r") as fin:
+    Validate the general shape of the table, expected columns, and each row. Also add
+    an additional column which records whether one or two FASTQ reads were found.
 
-        ## Check header
-        MIN_COLS = 2
-        # TODO nf-core: Update the column names for the input samplesheet
-        HEADER = ["sample", "fastq_1", "fastq_2"]
-        header = [x.strip('"') for x in fin.readline().strip().split(",")]
-        if header[: len(HEADER)] != HEADER:
-            print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
-            sys.exit(1)
+    Args:
+        file_in (pathlib.Path): The given tabular samplesheet. The format can be either
+            CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``.
+        file_out (pathlib.Path): Where the validated and transformed samplesheet should
+            be created; always in CSV format.
 
-        ## Check sample entries
-        for line in fin:
-            lspl = [x.strip().strip('"') for x in line.strip().split(",")]
-
-            # Check valid number of columns per row
-            if len(lspl) < len(HEADER):
-                print_error(
-                    "Invalid number of columns (minimum = {})!".format(len(HEADER)),
-                    "Line",
-                    line,
-                )
-            num_cols = len([x for x in lspl if x])
-            if num_cols < MIN_COLS:
-                print_error(
-                    "Invalid number of populated columns (minimum = {})!".format(MIN_COLS),
-                    "Line",
-                    line,
-                )
-
-            ## Check sample name entries
-            sample, fastq_1, fastq_2 = lspl[: len(HEADER)]
-            sample = sample.replace(" ", "_")
-            if not sample:
-                print_error("Sample entry has not been specified!", "Line", line)
-
-            ## Check FastQ file extension
-            for fastq in [fastq_1, fastq_2]:
-                if fastq:
-                    if fastq.find(" ") != -1:
-                        print_error("FastQ file contains spaces!", "Line", line)
-                    if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"):
-                        print_error(
-                            "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!",
-                            "Line",
-                            line,
-                        )
-
-            ## Auto-detect paired-end/single-end
-            sample_info = []  ## [single_end, fastq_1, fastq_2]
-            if sample and fastq_1 and fastq_2:  ## Paired-end short reads
-                sample_info = ["0", fastq_1, fastq_2]
-            elif sample and fastq_1 and not fastq_2:  ## Single-end short reads
-                sample_info = ["1", fastq_1, fastq_2]
-            else:
-                print_error("Invalid combination of columns provided!", "Line", line)
-
-            ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] }
-            if sample not in sample_mapping_dict:
-                sample_mapping_dict[sample] = [sample_info]
-            else:
-                if sample_info in sample_mapping_dict[sample]:
-                    print_error("Samplesheet contains duplicate rows!", "Line", line)
-                else:
-                    sample_mapping_dict[sample].append(sample_info)
-
-    ## Write validated samplesheet with appropriate columns
-    if len(sample_mapping_dict) > 0:
-        out_dir = os.path.dirname(file_out)
-        make_dir(out_dir)
-        with open(file_out, "w") as fout:
-            fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n")
-            for sample in sorted(sample_mapping_dict.keys()):
-
-                ## Check that multiple runs of the same sample are of the same datatype
-                if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]):
-                    print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample))
-
-                for idx, val in enumerate(sample_mapping_dict[sample]):
-                    fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n")
-    else:
-        print_error("No entries to process!", "Samplesheet: {}".format(file_in))
-
-
-def main(args=None):
-    args = parse_args(args)
-    check_samplesheet(args.FILE_IN, args.FILE_OUT)
+    Example:
+        This function checks that the samplesheet follows the following structure,
+        see also the `viral recon samplesheet`_::
+
+            sample,fastq_1,fastq_2
+            SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz
+            SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz
+            SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,
+
+    .. _viral recon samplesheet:
+        https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
+
+    """
+    required_columns = {"sample", "fastq_1", "fastq_2"}
+    # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`.
+    with file_in.open(newline="") as in_handle:
+        reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle))
+        # Validate the existence of the expected header columns.
+        if not required_columns.issubset(reader.fieldnames):
+            logger.critical(f"The sample sheet **must** contain the column headers: {', '.join(required_columns)}.")
+            sys.exit(1)
+        # Validate each row.
+        checker = RowChecker()
+        for i, row in enumerate(reader):
+            try:
+                checker.validate_and_transform(row)
+            except AssertionError as error:
+                logger.critical(f"{str(error)} On line {i + 2}.")
+                sys.exit(1)
+        checker.validate_unique_samples()
+    header = list(reader.fieldnames)
+    header.insert(1, "single_end")
+    # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`.
+    with file_out.open(mode="w", newline="") as out_handle:
+        writer = csv.DictWriter(out_handle, header, delimiter=",")
+        writer.writeheader()
+        for row in checker.modified:
+            writer.writerow(row)
+
+
+def parse_args(argv=None):
+    """Define and immediately parse command line arguments."""
+    parser = argparse.ArgumentParser(
+        description="Validate and transform a tabular samplesheet.",
+        epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv",
+    )
+    parser.add_argument(
+        "file_in",
+        metavar="FILE_IN",
+        type=Path,
+        help="Tabular input samplesheet in CSV or TSV format.",
+    )
+    parser.add_argument(
+        "file_out",
+        metavar="FILE_OUT",
+        type=Path,
+        help="Transformed output samplesheet in CSV format.",
+    )
+    parser.add_argument(
+        "-l",
+        "--log-level",
+        help="The desired log level (default WARNING).",
+        choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"),
+        default="WARNING",
+    )
+    return parser.parse_args(argv)
+
+
+def main(argv=None):
+    """Coordinate argument parsing and program execution."""
+    args = parse_args(argv)
+    logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s")
+    if not args.file_in.is_file():
+        logger.error(f"The given input file {args.file_in} was not found!")
+        sys.exit(2)
+    args.file_out.parent.mkdir(parents=True, exist_ok=True)
+    check_samplesheet(args.file_in, args.file_out)
 
 
 if __name__ == "__main__":
diff --git a/conf/base.config b/conf/base.config
index f2d66794..906b4d28 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -1,7 +1,7 @@
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     nf-core/rnafusion Nextflow base config file
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     A 'blank slate' config file, appropriate for general use on most high performance
     compute environments. Assumes that all software is installed and available on
     the PATH. Runs in `local` mode - all jobs will be run on the logged in environment.
diff --git a/conf/igenomes.config b/conf/igenomes.config
index 855948de..7a1b3ac6 100644
--- a/conf/igenomes.config
+++ b/conf/igenomes.config
@@ -1,7 +1,7 @@
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Nextflow config file for iGenomes paths
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Defines reference genomes using iGenome paths.
     Can be used by any config that customises the base path using:
         $params.igenomes_base / --igenomes_base
@@ -13,7 +13,7 @@ params {
     genomes {
         'GRCh37' {
             fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
@@ -26,7 +26,7 @@ params {
         }
         'GRCh38' {
             fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
@@ -38,7 +38,7 @@ params {
         }
         'GRCm38' {
             fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
@@ -51,7 +51,7 @@ params {
         }
         'TAIR10' {
             fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
@@ -62,7 +62,7 @@ params {
         }
         'EB2' {
             fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
@@ -72,7 +72,7 @@ params {
         }
         'UMD3.1' {
             fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
@@ -83,7 +83,7 @@ params {
         }
         'WBcel235' {
             fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
@@ -94,7 +94,7 @@ params {
         }
         'CanFam3.1' {
             fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
@@ -105,7 +105,7 @@ params {
         }
         'GRCz10' {
             fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
@@ -115,7 +115,7 @@ params {
         }
         'BDGP6' {
             fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
@@ -126,7 +126,7 @@ params {
         }
         'EquCab2' {
             fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
@@ -137,7 +137,7 @@ params {
         }
         'EB1' {
             fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
@@ -147,7 +147,7 @@ params {
         }
         'Galgal4' {
             fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
@@ -157,7 +157,7 @@ params {
         }
         'Gm01' {
             fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
@@ -167,7 +167,7 @@ params {
         }
         'Mmul_1' {
             fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
@@ -178,7 +178,7 @@ params {
         }
         'IRGSP-1.0' {
             fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
@@ -188,7 +188,7 @@ params {
         }
         'CHIMP2.1.4' {
             fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
@@ -199,7 +199,7 @@ params {
         }
         'Rnor_5.0' {
             fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
@@ -209,7 +209,7 @@ params {
         }
         'Rnor_6.0' {
             fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
@@ -219,7 +219,7 @@ params {
         }
         'R64-1-1' {
             fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
@@ -230,7 +230,7 @@ params {
         }
         'EF2' {
             fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
@@ -242,7 +242,7 @@ params {
         }
         'Sbi1' {
             fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
@@ -252,7 +252,7 @@ params {
         }
         'Sscrofa10.2' {
             fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
@@ -263,7 +263,7 @@ params {
         }
         'AGPv3' {
             fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
@@ -273,7 +273,7 @@ params {
         }
         'hg38' {
             fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
@@ -285,7 +285,7 @@ params {
         }
         'hg19' {
             fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
@@ -298,7 +298,7 @@ params {
         }
         'mm10' {
             fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
@@ -311,7 +311,7 @@ params {
         }
         'bosTau8' {
             fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
@@ -321,7 +321,7 @@ params {
         }
         'ce10' {
             fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
@@ -333,7 +333,7 @@ params {
         }
         'canFam3' {
             fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
@@ -344,7 +344,7 @@ params {
         }
         'danRer10' {
             fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
@@ -355,7 +355,7 @@ params {
         }
         'dm6' {
             fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
@@ -366,7 +366,7 @@ params {
         }
         'equCab2' {
             fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
@@ -377,7 +377,7 @@ params {
         }
         'galGal4' {
             fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
@@ -388,7 +388,7 @@ params {
         }
         'panTro4' {
             fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
@@ -399,7 +399,7 @@ params {
         }
         'rn6' {
             fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
@@ -409,7 +409,7 @@ params {
         }
         'sacCer3' {
             fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
@@ -419,7 +419,7 @@ params {
         }
         'susScr3' {
             fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/"
             bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
             star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
             bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
diff --git a/conf/modules.config b/conf/modules.config
index a0506a4d..da58a5d8 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -1,12 +1,12 @@
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Config file for defining DSL2 per module options and publishing paths
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Available keys to override module options:
-        ext.args            = Additional arguments appended to command in module.
-        ext.args2           = Second set of arguments appended to command in module (multi-tool modules).
-        ext.args3           = Third set of arguments appended to command in module (multi-tool modules).
-        ext.prefix          = File name prefix for output files.
+        ext.args   = Additional arguments appended to command in module.
+        ext.args2  = Second set of arguments appended to command in module (multi-tool modules).
+        ext.args3  = Third set of arguments appended to command in module (multi-tool modules).
+        ext.prefix = File name prefix for output files.
 ----------------------------------------------------------------------------------------
 */
 
@@ -14,14 +14,14 @@ process {
 
     publishDir = [
         path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
-        mode: 'copy',
+        mode: params.publish_dir_mode,
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
     withName: SAMPLESHEET_CHECK {
         publishDir = [
             path: { "${params.outdir}/pipeline_info" },
-            mode: 'copy',
+            mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
@@ -33,7 +33,7 @@ process {
     withName: CUSTOM_DUMPSOFTWAREVERSIONS {
         publishDir = [
             path: { "${params.outdir}/pipeline_info" },
-            mode: 'copy',
+            mode: params.publish_dir_mode,
             pattern: '*_versions.yml'
         ]
     }
diff --git a/conf/test.config b/conf/test.config
index d10f8c89..796db260 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -1,11 +1,11 @@
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Nextflow config file for running minimal tests
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Defines input files and everything required to run a fast and simple pipeline test.
 
     Use as follows:
-        nextflow run nf-core/rnafusion -profile test,<docker/singularity>
+        nextflow run nf-core/rnafusion -profile test,<docker/singularity> --outdir <OUTDIR>
 
 ----------------------------------------------------------------------------------------
 */
diff --git a/conf/test_full.config b/conf/test_full.config
index 260cb93f..e2522d72 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -1,11 +1,11 @@
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Nextflow config file for running full-size tests
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Defines input files and everything required to run a full size pipeline test.
 
     Use as follows:
-        nextflow run nf-core/rnafusion -profile test_full,<docker/singularity>
+        nextflow run nf-core/rnafusion -profile test_full,<docker/singularity> --outdir <OUTDIR>
 
 ----------------------------------------------------------------------------------------
 */
diff --git a/docs/images/nf-core-rnafusion_logo_dark.png b/docs/images/nf-core-rnafusion_logo_dark.png
index dee3db82069b61e9a5380d940355f47ff565057c..598d1e22bc313c63210e1738da0b746cc722e40a 100644
GIT binary patch
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diff --git a/docs/images/nf-core-rnafusion_logo_light.png b/docs/images/nf-core-rnafusion_logo_light.png
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diff --git a/docs/usage.md b/docs/usage.md
index 0126fca9..d1e3eb21 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -57,7 +57,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p
 The typical command for running the pipeline is as follows:
 
 ```console
-nextflow run nf-core/rnafusion --input samplesheet.csv --genome GRCh37 -profile docker
+nextflow run nf-core/rnafusion --input samplesheet.csv  --outdir <OUTDIR> --genome GRCh37 -profile docker
 ```
 
 This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
@@ -141,11 +141,11 @@ Whilst the default requirements set within the pipeline will hopefully work for
 For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue:
 
 ```console
-[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
-Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
+[62/149eb0] NOTE: Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
+Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
 
 Caused by:
-    Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
+    Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
 
 Command executed:
     STAR \
@@ -169,17 +169,24 @@ Work dir:
 Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
 ```
 
-To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
+To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN).
+We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/software/star/align/main.nf`.
+If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9).
+The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements.
+The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB.
+Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB.
+The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
 
 ```nextflow
 process {
-    withName: STAR_ALIGN {
+    withName: 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN' {
         memory = 100.GB
     }
 }
 ```
 
-> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow.
+> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden.
+> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
 
 ### Updating containers
 
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index 40ab65f2..b3d092f8 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -27,7 +27,7 @@ class NfcoreSchema {
     /* groovylint-disable-next-line UnusedPrivateMethodParameter */
     public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') {
         def has_error = false
-        //=====================================================================//
+        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
         // Check for nextflow core params and unexpected params
         def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text
         def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
@@ -135,7 +135,7 @@ class NfcoreSchema {
             }
         }
 
-        //=====================================================================//
+        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
         // Validate parameters against the schema
         InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream()
         JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream))
diff --git a/lib/Utils.groovy b/lib/Utils.groovy
index 1b88aec0..28567bd7 100755
--- a/lib/Utils.groovy
+++ b/lib/Utils.groovy
@@ -29,12 +29,12 @@ class Utils {
         conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults'))
 
         if (conda_check_failed) {
-            log.warn "=============================================================================\n" +
+            log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
                 "  There is a problem with your Conda configuration!\n\n" +
                 "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
                 "  Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" +
                 "  NB: The order of the channels matters!\n" +
-                "==================================================================================="
+                "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
         }
     }
 }
diff --git a/lib/WorkflowRnafusion.groovy b/lib/WorkflowRnafusion.groovy
index e9d53d8a..74705064 100755
--- a/lib/WorkflowRnafusion.groovy
+++ b/lib/WorkflowRnafusion.groovy
@@ -48,11 +48,11 @@ class WorkflowRnafusion {
     //
     private static void genomeExistsError(params, log) {
         if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
-            log.error "=============================================================================\n" +
+            log.error "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
                 "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
                 "  Currently, the available genome keys are:\n" +
                 "  ${params.genomes.keySet().join(", ")}\n" +
-                "==================================================================================="
+                "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
             System.exit(1)
         }
     }
diff --git a/main.nf b/main.nf
index 2b33ffe8..b2c34856 100644
--- a/main.nf
+++ b/main.nf
@@ -1,8 +1,8 @@
 #!/usr/bin/env nextflow
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     nf-core/rnafusion
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Github : https://github.com/nf-core/rnafusion
     Website: https://nf-co.re/rnafusion
     Slack  : https://nfcore.slack.com/channels/rnafusion
@@ -12,25 +12,25 @@
 nextflow.enable.dsl = 2
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     GENOME PARAMETER VALUES
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     VALIDATE & PRINT PARAMETER SUMMARY
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 WorkflowMain.initialise(workflow, params, log)
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     NAMED WORKFLOW FOR PIPELINE
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 include { RNAFUSION } from './workflows/rnafusion'
@@ -43,9 +43,9 @@ workflow NFCORE_RNAFUSION {
 }
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN ALL WORKFLOWS
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 //
@@ -57,7 +57,7 @@ workflow {
 }
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     THE END
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
diff --git a/nextflow.config b/nextflow.config
index 497e7ea2..270f9528 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -1,7 +1,7 @@
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     nf-core/rnafusion Nextflow config file
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Default config options for all compute environments
 ----------------------------------------------------------------------------------------
 */
@@ -24,8 +24,9 @@ params {
     max_multiqc_email_size     = '25.MB'
 
     // Boilerplate options
-    outdir                     = './results'
+    outdir                     = null
     tracedir                   = "${params.outdir}/pipeline_info"
+    publish_dir_mode           = 'copy'
     email                      = null
     email_on_fail              = null
     plaintext_email            = false
@@ -62,6 +63,15 @@ try {
     System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
 }
 
+// Load nf-core/rnafusion custom profiles from different institutions.
+// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs!
+// try {
+//   includeConfig "${params.custom_config_base}/pipeline/rnafusion.config"
+// } catch (Exception e) {
+//   System.err.println("WARNING: Could not load nf-core/config/rnafusion profiles: ${params.custom_config_base}/pipeline/rnafusion.config")
+// }
+
+
 profiles {
     debug { process.beforeScript = 'echo $HOSTNAME' }
     conda {
@@ -121,7 +131,7 @@ if (!params.igenomes_ignore) {
 }
 
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container
-// The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. 
+// The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container.
 // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable.
 
 env {
@@ -154,7 +164,7 @@ dag {
 
 manifest {
     name            = 'nf-core/rnafusion'
-    author          = 'Martin Proks'
+    author          = 'Martin Proks, @praveenraj2018'
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
     mainScript      = 'main.nf'
diff --git a/nextflow_schema.json b/nextflow_schema.json
index f602f89c..99a42236 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -11,7 +11,8 @@
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
             "required": [
-                "input"
+                "input",
+                "outdir"
             ],
             "properties": {
                 "input": {
@@ -26,8 +27,8 @@
                 },
                 "outdir": {
                     "type": "string",
-                    "description": "Path to the output directory where the results will be saved.",
-                    "default": "./results",
+                    "format": "directory-path",
+                    "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.",
                     "fa_icon": "fas fa-folder-open"
                 },
                 "email": {
@@ -178,6 +179,22 @@
                     "fa_icon": "fas fa-question-circle",
                     "hidden": true
                 },
+                "publish_dir_mode": {
+                    "type": "string",
+                    "default": "copy",
+                    "description": "Method used to save pipeline results to output directory.",
+                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
+                    "fa_icon": "fas fa-copy",
+                    "enum": [
+                        "symlink",
+                        "rellink",
+                        "link",
+                        "copy",
+                        "copyNoFollow",
+                        "move"
+                    ],
+                    "hidden": true
+                },
                 "email_on_fail": {
                     "type": "string",
                     "description": "Email address for completion summary, only when pipeline fails.",
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index cddcbb3c..0aecf87f 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -12,7 +12,7 @@ workflow INPUT_CHECK {
     SAMPLESHEET_CHECK ( samplesheet )
         .csv
         .splitCsv ( header:true, sep:',' )
-        .map { create_fastq_channels(it) }
+        .map { create_fastq_channel(it) }
         .set { reads }
 
     emit:
@@ -21,22 +21,24 @@ workflow INPUT_CHECK {
 }
 
 // Function to get list of [ meta, [ fastq_1, fastq_2 ] ]
-def create_fastq_channels(LinkedHashMap row) {
+def create_fastq_channel(LinkedHashMap row) {
+    // create meta map
     def meta = [:]
-    meta.id           = row.sample
-    meta.single_end   = row.single_end.toBoolean()
+    meta.id         = row.sample
+    meta.single_end = row.single_end.toBoolean()
 
-    def array = []
+    // add path(s) of the fastq file(s) to the meta map
+    def fastq_meta = []
     if (!file(row.fastq_1).exists()) {
         exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}"
     }
     if (meta.single_end) {
-        array = [ meta, [ file(row.fastq_1) ] ]
+        fastq_meta = [ meta, [ file(row.fastq_1) ] ]
     } else {
         if (!file(row.fastq_2).exists()) {
             exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}"
         }
-        array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ]
+        fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ]
     }
-    return array
+    return fastq_meta
 }
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 4dad9839..acc8b85f 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -1,7 +1,7 @@
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     VALIDATE INPUTS
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
@@ -18,18 +18,18 @@ for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true
 if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' }
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     CONFIG FILES
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 ch_multiqc_config        = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true)
 ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     IMPORT LOCAL MODULES/SUBWORKFLOWS
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 //
@@ -38,9 +38,9 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 include { INPUT_CHECK } from '../subworkflows/local/input_check'
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 //
@@ -51,9 +51,9 @@ include { MULTIQC                     } from '../modules/nf-core/modules/multiqc
 include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main'
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 // Info required for completion email and summary
@@ -104,9 +104,9 @@ workflow RNAFUSION {
 }
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     COMPLETION EMAIL AND SUMMARY
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 workflow.onComplete {
@@ -117,7 +117,7 @@ workflow.onComplete {
 }
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     THE END
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */

From f20ce47b1ed1b4c731f296b117ad835e81467c20 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Mar 2022 09:29:33 +0100
Subject: [PATCH 198/359] yaml linting

---
 .github/workflows/awstest.yml                 |  1 -
 .github/workflows/branch.yml                  |  1 -
 .github/workflows/linting.yml                 |  1 -
 .github/workflows/linting_comment.yml         |  2 --
 .gitpod.yml                                   |  2 +-
 modules/local/fusioncatcher/detect/meta.yml   |  6 +++---
 modules/local/fusioncatcher/download/meta.yml |  6 +++---
 modules/local/fusioninspector/meta.yml        |  6 +++---
 modules/local/fusionreport/detect/meta.yml    | 12 ++++++------
 modules/local/fusionreport/download/meta.yml  |  6 +++---
 10 files changed, 19 insertions(+), 24 deletions(-)

diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index ae430044..0662b257 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -12,7 +12,6 @@ jobs:
     steps:
       - name: Launch workflow via tower
         uses: nf-core/tower-action@v3
-        
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 37b7dea8..c58de07d 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -43,4 +43,3 @@ jobs:
             Thanks again for your contribution!
           repo-token: ${{ secrets.GITHUB_TOKEN }}
           allow-repeats: false
-
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index fda934c0..29088e5b 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -138,4 +138,3 @@ jobs:
             lint_log.txt
             lint_results.md
             PR_number.txt
-
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 44d72994..04758f61 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -1,4 +1,3 @@
-
 name: nf-core linting comment
 # This workflow is triggered after the linting action is complete
 # It posts an automated comment to the PR, even if the PR is coming from a fork
@@ -27,4 +26,3 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
           path: linting-logs/lint_results.md
-
diff --git a/.gitpod.yml b/.gitpod.yml
index b7d4cee1..6ea5d5f2 100644
--- a/.gitpod.yml
+++ b/.gitpod.yml
@@ -1,7 +1,7 @@
 image: nfcore/gitpod:latest
 
 vscode:
-  extensions: # based on nf-core.nf-core-extensionpack
+  extensions:                                # based on nf-core.nf-core-extensionpack
     - codezombiech.gitignore                 # Language support for .gitignore files
     # - cssho.vscode-svgviewer                 # SVG viewer
     - davidanson.vscode-markdownlint         # Markdown/CommonMark linting and style checking for Visual Studio Code
diff --git a/modules/local/fusioncatcher/detect/meta.yml b/modules/local/fusioncatcher/detect/meta.yml
index 8081142b..795587fd 100644
--- a/modules/local/fusioncatcher/detect/meta.yml
+++ b/modules/local/fusioncatcher/detect/meta.yml
@@ -45,9 +45,9 @@ output:
       description: Log of fusion results
       pattern: "*.fusioncatcher.log"
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 
 authors:
   - "@praveenraj2018. @rannick"
diff --git a/modules/local/fusioncatcher/download/meta.yml b/modules/local/fusioncatcher/download/meta.yml
index b24b5e82..dcc7eb9f 100644
--- a/modules/local/fusioncatcher/download/meta.yml
+++ b/modules/local/fusioncatcher/download/meta.yml
@@ -13,9 +13,9 @@ tools:
 
 output:
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
   - reference:
       type: directory
       description: Path to fusioncatcher references
diff --git a/modules/local/fusioninspector/meta.yml b/modules/local/fusioninspector/meta.yml
index 5002fe28..acf3e0bb 100644
--- a/modules/local/fusioninspector/meta.yml
+++ b/modules/local/fusioninspector/meta.yml
@@ -29,9 +29,9 @@ input:
 # TODO complete output
 output:
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
   - reference:
       type: directory
       description: Genome resource path
diff --git a/modules/local/fusionreport/detect/meta.yml b/modules/local/fusionreport/detect/meta.yml
index 72101495..aeb23dc5 100644
--- a/modules/local/fusionreport/detect/meta.yml
+++ b/modules/local/fusionreport/detect/meta.yml
@@ -43,13 +43,13 @@ input:
 
 output:
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
   - fusion_list:
-        type: file
-        description: File containing the summary of all fusions fed-in
-        pattern: "*.tsv"
+      type: file
+      description: File containing the summary of all fusions fed-in
+      pattern: "*.tsv"
   - report:
       type: file
       description: HTML files
diff --git a/modules/local/fusionreport/download/meta.yml b/modules/local/fusionreport/download/meta.yml
index d4bab11a..3b297fd7 100644
--- a/modules/local/fusionreport/download/meta.yml
+++ b/modules/local/fusionreport/download/meta.yml
@@ -23,9 +23,9 @@ input:
 
 output:
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
   - reference:
       type: directory
       description: directory containing the genome resource files required for fusioncatcher

From 9c574f720180e5fa6a696b83980c7791e018e0bb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Mar 2022 09:48:26 +0100
Subject: [PATCH 199/359] add branch.yml linting.yml linting_comment.yml to
 files unchanged to avoid template check

---
 .github/workflows/branch.yml | 1 -
 .nf-core.yml                 | 6 ++++++
 2 files changed, 6 insertions(+), 1 deletion(-)

diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index c58de07d..ccb7211b 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -15,7 +15,6 @@ jobs:
         run: |
           { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
 
-
       # If the above check failed, post a comment on the PR explaining the failure
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
       - name: Post PR comment
diff --git a/.nf-core.yml b/.nf-core.yml
index 3805dc81..7036b988 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1 +1,7 @@
 repository_type: pipeline
+lint:
+  files_unchanged:
+    # Added to ignore linting error: actions/setup-node@v1 until fixed release
+    - .github/workflows/linting.yml
+    - .github/workflows/branch.yml
+    - .github/workflows/linting_comment.yml

From 6181352bb958de8864da629da4bf0d0076b54638 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 18 Mar 2022 08:58:01 +0100
Subject: [PATCH 200/359] CITATIONS.md completed

---
 CITATIONS.md | 27 +++++++++++++++++++++++++++
 1 file changed, 27 insertions(+)

diff --git a/CITATIONS.md b/CITATIONS.md
index 702b64c9..7c1a47f3 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -15,6 +15,33 @@
 * [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
     > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
 
+* [Arriba](https://github.com/suhrig/arriba)
+    > Uhrig S, Ellermann J, Walther T, Burkhardt P, Fröhlich M, Hutter B, Toprak UH, Neumann O, Stenzinger A, Scholl C, Fröhling S, Brors B. Accurate and efficient detection of gene fusions from RNA sequencing data.
+    Genome Research. 2021 Mar 31;448-460. doi: 10.1101/gr.257246.119. Epub 2021 Jan 13. PubMed PMID: 33441414; PubMed Central PMCID: PMC7919457.
+
+* [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
+    > Nicorici D, Satalan M, Edgren H, Kangaspeska S, Murumagi A, Kallioniemi O, Virtanen S, Kilkku O. FusionCatcher – a tool for finding somatic fusion genes in paired-end RNA-sequencing data. BioRxiv, 2014 Nov. doi: 10.1101/011650.
+
+* [Fusion-report](https://github.com/matq007/fusion-report)
+
+* [Kallisto](https://pachterlab.github.io/kallisto/)
+    > Bray NL, Pimentel H, Melsted P, Pachter L. Near-optimal probabilistic RNA-seq quantification. Nature Biotechnology 2016 Apr. 34, 525–527. doi:10.1038/nbt.3519. PMID: 27043002.
+
+* [Pizzly](https://github.com/pmelsted/pizzly)
+    Melsted P, Hateley S, Joseph IC, Pimentel H, Bray N, Pachter L. Fusion detection and quantification by pseudoalignment. BioRxiv, 2017 Jul. doi: 10.1101/166322.
+
+* [SAMtools](https://pubmed.ncbi.nlm.nih.gov/19505943/)
+    > Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PubMed PMID: 19505943; PubMed Central PMCID: PMC2723002.
+
+* [Squid](https://github.com/Kingsford-Group/squid)
+    > Ma C, Shao M, Kingsford C. SQUID: transcriptomic structural variation detection from RNA-seq. Genome Biol 2028 Apr. 19, 52. doi: 10.1186/s13059-018-1421-5. PubMed PMID: 29650026. PubMed Central PMCID: PMC5896115.
+
+* [STAR](https://pubmed.ncbi.nlm.nih.gov/23104886/)
+    > Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner Bioinformatics. 2013 Jan 1;29(1):15-21. doi: 10.1093/bioinformatics/bts635. Epub 2012 Oct 25. PubMed PMID: 23104886; PubMed Central PMCID: PMC3530905.
+
+* [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)
+    > Haas BJ, Dobin A, Stransky N, Li B, Yang X, Tickle T, Bankapur A, Ganote C, Doak TG, Pochet N, Sun J, Wu CJ, Gingeras TR, Regev A. STAR-Fusion: Fast and Accurate Fusion Transcript Detection from RNA-Seq. BioRxiv 2017 Mar. doi: 10.1101/120295.
+
 ## Software packaging/containerisation tools
 
 * [Anaconda](https://anaconda.com)

From 080012680976b2adf24945fbf158efa5d99e531f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 18 Mar 2022 09:04:34 +0100
Subject: [PATCH 201/359] Update README

---
 README.md | 67 ++++++++++++++++++++++++++++++++++++++++++-------------
 1 file changed, 52 insertions(+), 15 deletions(-)

diff --git a/README.md b/README.md
index 6384f70b..04bd54dc 100644
--- a/README.md
+++ b/README.md
@@ -3,7 +3,8 @@
 [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+CI%22)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)
 [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)
-[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
+[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3946477)
+<!-- TODO update zenodo after release -->
 
 [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
@@ -22,21 +23,58 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 
 | Tool                                                                      |  Single-end reads  |  Version |
 | ------------------------------------------------------------------------- | :----------------: | :------: |
-| [Arriba](https://github.com/suhrig/arriba)                                |         :x:        |  `1.2.0` |
-| [EricScript](https://sites.google.com/site/bioericscript/getting-started) |         :x:        |  `0.5.5` |
-| [FusionCatcher](https://github.com/ndaniel/fusioncatcher)                 | :white_check_mark: |  `1.20`  |
-| [Fusion-Inspector](https://github.com/FusionInspector/FusionInspector)    |         :x:        |  `2.3.1` |
-| [fusion-report](https://github.com/matq007/fusion-report)                 |          -         |  `2.1.3` |
+| [Arriba](https://github.com/suhrig/arriba)                                |         :x:        |  `2.1.0` |
+| [FusionCatcher](https://github.com/ndaniel/fusioncatcher)                 | :white_check_mark: |  `1.33`  |
+| [Fusion-report](https://github.com/matq007/fusion-report)                 |          -         |  `2.1.5` |
 | [Pizzly](https://github.com/pmelsted/pizzly)                              |         :x:        | `0.37.3` |
 | [Squid](https://github.com/Kingsford-Group/squid)                         |         :x:        |   `1.5`  |
-| [Star-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: |  `1.9.1` |
+| [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: | `1.10.1` |
+<!-- TODO fusion-report: check version after release -->
 
 ## Pipeline summary
 
-<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
-
-1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
+#### Build references
+`--build_references` triggers a parallel workflow to build all references
+1. Download ensembl fasta and gtf files
+2. Create STAR index
+3. Download arriba references
+4. Download fusioncatcher references
+5. Download pizzly references (kallisto index)
+6. Download and build STAR-fusion references
+7. Download fusion-report DBs
+#### Main workflow
+
+1. Input samplesheet check
+2. Concatenate fastq files per sample
+3. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+4. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
+5. Arriba subworkflow
+    * STAR alignment
+    * Samtool sort
+    * Samtool index
+    * [Arriba](https://github.com/suhrig/arriba) fusion detection
+    * [Arriba](https://github.com/suhrig/arriba) visualisation
+6. Pizzly subworkflow
+    * [Kallisto](https://pachterlab.github.io/kallisto/) quantification
+    * [Pizzly](https://github.com/pmelsted/pizzly) fusion detection
+7. Squid subworkflow
+    * [STAR](https://github.com/alexdobin/STAR) alignment
+    * [Samtools view](http://www.htslib.org/): convert sam output from STAR to bam
+    * [Samtools sort](http://www.htslib.org/): bam output from STAR
+    * [Squid](https://github.com/Kingsford-Group/squid) fusion detection
+    * [Squid](https://github.com/Kingsford-Group/squid) annotate
+8. STAR-fusion subworkflow
+    * [STAR](https://github.com/alexdobin/STAR) alignment
+    * [STAR-fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
+9. Fusioncatcher subworkflow
+    * [Fusioncatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
+10. Fusion-report subworkflow
+    * Merge all fusions detected by the different tools
+    * [Fusion-report](https://github.com/matq007/fusion-report)
+11. FusionInspector subworkflow
+    * [FusionInspector](https://github.com/FusionInspector/FusionInspector)
+
+<!-- TODO Add QC steps, MultiQC details -->
 
 ## Quick Start
 
@@ -69,7 +107,7 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline [usag
 
 ## Credits
 
-nf-core/rnafusion was originally written by Martin Proks, @praveenraj2018
+nf-core/rnafusion was originally written by Martin Proks, @praveenraj2018, on the basis of work from Rickard Hammar&eacute;n.
 
 We thank the following people for their extensive assistance in the development of this pipeline:
 
@@ -83,10 +121,9 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 
 ## Citations
 
-<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->
-<!-- If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
+<!-- TODO update zenodo after release -->
+If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)
 
-<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
 
 You can cite the `nf-core` publication as follows:

From 3c1d6ea130fbc420325b35c0e572045b2a1df0e2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Sun, 20 Mar 2022 22:49:30 +0100
Subject: [PATCH 202/359] draft documentation, remove ericscript, bump ensembl
 version to 105

---
 README.md                                    |   4 +-
 bin/check_samplesheet.py                     |   2 -
 conf/genomes.config                          |   2 -
 conf/modules.config                          |   2 -
 docs/output.md                               | 131 +++++
 docs/usage.md                                | 491 +++----------------
 modules/local/fusioninspector/meta.yml       |   1 -
 modules/local/fusionreport/download/main.nf  |   3 -
 nextflow.config                              |   4 +-
 nextflow_schema.json                         |  41 +-
 subworkflows/local/arriba_workflow.nf        |   2 +-
 subworkflows/local/fusioncatcher_workflow.nf |   2 +-
 subworkflows/local/pizzly_workflow.nf        |   2 +-
 subworkflows/local/squid_workflow.nf         |   2 +-
 subworkflows/local/starfusion_workflow.nf    |   2 +-
 workflows/build_references.nf                |   5 -
 workflows/rnafusion.nf                       |   2 +-
 17 files changed, 217 insertions(+), 481 deletions(-)

diff --git a/README.md b/README.md
index 04bd54dc..2e4498b3 100644
--- a/README.md
+++ b/README.md
@@ -67,7 +67,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
     * [STAR](https://github.com/alexdobin/STAR) alignment
     * [STAR-fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
 9. Fusioncatcher subworkflow
-    * [Fusioncatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
+    * [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
 10. Fusion-report subworkflow
     * Merge all fusions detected by the different tools
     * [Fusion-report](https://github.com/matq007/fusion-report)
@@ -107,7 +107,7 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline [usag
 
 ## Credits
 
-nf-core/rnafusion was originally written by Martin Proks, @praveenraj2018, on the basis of work from Rickard Hammar&eacute;n.
+nf-core/rnafusion was originally written by Martin Proks, @praveenraj2018, on the basis of work from Rickard Hammarén.
 
 We thank the following people for their extensive assistance in the development of this pipeline:
 
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 646150dc..a3352c71 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -1,7 +1,5 @@
 #!/usr/bin/env python
 
-# TODO nf-core: Update the script to check the samplesheet
-# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
 
 import os
 import sys
diff --git a/conf/genomes.config b/conf/genomes.config
index 5bdd6d58..e4914bd5 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -16,7 +16,6 @@ params {
             transcript                    = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
             arriba_ref                    = "${params.genomes_base}/arriba"
             ensembl_ref                   = "${params.genomes_base}/ensembl"
-            ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
             fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
             pizzly_ref                    = "${params.genomes_base}/pizzly"
             squid_ref                     = "${params.genomes_base}/squid"
@@ -30,7 +29,6 @@ params {
             transcript                    = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.cdna.all.fa.gz"
             arriba_ref                    = "${params.genomes_base}/arriba"
             ensembl_ref                   = "${params.genomes_base}/ensembl"
-            ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
             fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
             pizzly_ref                    = "${params.genomes_base}/pizzly"
             squid_ref                     = "${params.genomes_base}/squid"
diff --git a/conf/modules.config b/conf/modules.config
index 65ce1b06..2f258012 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -153,7 +153,6 @@ process {
         ext.args = { "-Sb -o ${meta.id}_chimeric.bam" }
     }
 
-//TODO document changes from previous rnafusion version
     withName: STAR_FOR_ARRIBA {
         publishDir = [
             path: { "${params.outdir}/star_for_arriba" },
@@ -195,7 +194,6 @@ process {
         --readFilesCommand zcat'
     }
 
-//TODO document changes from previous rnafusion version to use star-fusion recommendations
     withName: STAR_FOR_STARFUSION {
         publishDir = [
             path: { "${params.outdir}/star_for_starfusion" },
diff --git a/docs/output.md b/docs/output.md
index cc7bfdad..a83b5b99 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -10,10 +10,141 @@ The directories listed below will be created in the results directory after the
 
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
+* [Download and build references](#references) - Build references needed to run the rest of the pipeline
+* [STAR](#star) - Alignment for arriba, squid and STAR-fusion
+* [cat](#cat) - Concatenated fastq files per sample ID
+* [Arriba](#arriba) - Arriba fusion detection
+* [Pizzly](#pizzly) - Pizzly fusion detection
+* [Squid](#squid) - Squid fusion detection
+* [STAR-fusion](#starfusion) - STAR-fusion fusion detection
+* [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
+* [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report
+* [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman and FusionGBD databases
+* [FusionInspectior](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
 * [FastQC](#fastqc) - Raw read QC
 * [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
 * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
+
+
+<!-- 1. Input samplesheet check
+2. Concatenate fastq files per sample
+3. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+4. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
+5. Arriba subworkflow
+    * STAR alignment
+    * Samtool sort
+    * Samtool index
+    * [Arriba](https://github.com/suhrig/arriba) fusion detection
+    * [Arriba](https://github.com/suhrig/arriba) visualisation
+6. Pizzly subworkflow
+    * [Kallisto](https://pachterlab.github.io/kallisto/) quantification
+    * [Pizzly](https://github.com/pmelsted/pizzly) fusion detection
+7. Squid subworkflow
+    * [STAR](https://github.com/alexdobin/STAR) alignment
+    * [Samtools view](http://www.htslib.org/): convert sam output from STAR to bam
+    * [Samtools sort](http://www.htslib.org/): bam output from STAR
+    * [Squid](https://github.com/Kingsford-Group/squid) fusion detection
+    * [Squid](https://github.com/Kingsford-Group/squid) annotate
+8. STAR-fusion subworkflow
+    * [STAR](https://github.com/alexdobin/STAR) alignment
+    * [STAR-fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
+9. Fusioncatcher subworkflow
+    * [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
+10. Fusion-report subworkflow
+    * Merge all fusions detected by the different tools
+    * [Fusion-report](https://github.com/matq007/fusion-report)
+11. FusionInspector subworkflow
+    * [FusionInspector](https://github.com/FusionInspector/FusionInspector) -->
+
+<!-- TODO details on each step -->
+### Download and build references
+
+<details markdown="1">
+<summary>Output files</summary>
+
+ * `genomes_base/`
+    * `arriba`
+
+<!-- TODO complete list of reference output files -->
+
+
+### STAR
+
+<details markdown="1">
+<summary>Output files</summary>
+
+<!-- TODO complete list of output files -->
+
+STAR is run 3 times:
+
+For arriba with the parameters:
+
+>`--readFilesCommand zcat \
+--outSAMtype BAM Unsorted \
+--outSAMunmapped Within \
+--outBAMcompression 0 \
+--outFilterMultimapNmax 50 \
+--peOverlapNbasesMin 10 \
+--alignSplicedMateMapLminOverLmate 0.5 \
+--alignSJstitchMismatchNmax 5 -1 5 5 \
+--chimSegmentMin 10 \
+--chimOutType WithinBAM HardClip \
+--chimJunctionOverhangMin 10 \
+--chimScoreDropMax 30 \
+--chimScoreJunctionNonGTAG 0 \
+--chimScoreSeparation 1 \
+--chimSegmentReadGapMax 3 \
+--chimMultimapNmax 50`
+
+For squid with the parameters:
+>`--twopassMode Basic \
+--chimOutType SeparateSAMold \
+--chimSegmentMin 20 \
+--chimJunctionOverhangMin 12 \
+--alignSJDBoverhangMin 10 \
+--outReadsUnmapped Fastx \
+--outSAMstrandField intronMotif \
+--outSAMtype BAM SortedByCoordinate \
+--readFilesCommand zcat`
+
+For STAR-fusion with the parameters:
+>`--twopassMode Basic \
+--outReadsUnmapped None \
+--readFilesCommand zcat \
+--outSAMstrandField intronMotif \
+--outSAMunmapped Within \
+--chimSegmentMin 12 \
+--chimJunctionOverhangMin 8 \
+--chimOutJunctionFormat 1 \
+--alignSJDBoverhangMin 10 \
+--alignMatesGapMax 100000 \
+--alignIntronMax 100000 \
+--alignSJstitchMismatchNmax 5 -1 5 5 \
+--chimMultimapScoreRange 3 \
+--chimScoreJunctionNonGTAG -4 \
+--chimMultimapNmax 20 \
+--chimNonchimScoreDropMin 10 \
+--peOverlapNbasesMin 12 \
+--peOverlapMMp 0.1 \
+--alignInsertionFlush Right \
+--alignSplicedMateMapLminOverLmate 0 \
+--alignSplicedMateMapLmin 30 \
+--chimOutType Junctions`
+
+
+
+Alignment for arriba, squid and STAR-fusion
+* [cat](#cat) - Concatenated fastq files per sample ID
+* [Arriba](#arriba) - Arriba fusion detection
+* [Pizzly](#pizzly) - Pizzly fusion detection
+* [Squid](#squid) - Squid fusion detection
+* [STAR-fusion](#starfusion) - STAR-fusion fusion detection
+* [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
+* [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report
+* [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman and FusionGBD databases
+* [FusionInspectior](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
+
 ### FastQC
 
 <details markdown="1">
diff --git a/docs/usage.md b/docs/usage.md
index aa165a7d..228accad 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -3,150 +3,57 @@
 ## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/rnafusion/usage](https://nf-co.re/rnafusion/usage)
 
 > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._
-
 ## Introduction
 
-- [:warning: Please read this documentation on the nf-core website: https://nf-co.re/rnafusion/usage](#warning-please-read-this-documentation-on-the-nf-core-website-httpsnf-corernafusionusage)
-- [Introduction](#introduction)
-- [Download references](#download-references)
-    - [Download all references](#download-all-references)
-    - [Download specific references](#download-specific-references)
-    - [Download and build CTAT](#download-and-build-ctat)
-    - [Download GRCh37 references](#download-grch37-references)
-    - [Tool reference requirements](#tool-reference-requirements)
-- [Running the pipeline](#running-the-pipeline)
-    - [Updating the pipeline](#updating-the-pipeline)
-    - [Reproducibility](#reproducibility)
-- [Core Nextflow arguments](#core-nextflow-arguments)
-    - [`-profile`](#-profile)
-    - [`-resume`](#-resume)
-    - [`-c`](#-c)
-        - [Custom resource requests](#custom-resource-requests)
-    - [Running in the background](#running-in-the-background)
-        - [Nextflow memory requirements](#nextflow-memory-requirements)
-- [Pipeline specific arguments](#pipeline-specific-arguments)
-    - [--input](#--input)
-    - [--single_end](#--single_end)
-    - [Tool flags](#tool-flags)
-    - [--arriba](#--arriba)
-    - [--ericscript](#--ericscript)
-    - [--fusioncatcher](#--fusioncatcher)
-    - [--fusion_report](#--fusion_report)
-    - [--pizzly](#--pizzly)
-    - [--squid](#--squid)
-    - [--star_fusion](#--star_fusion)
-- [Visualization flags](#visualization-flags)
-    - [--arriba_vis](#--arriba_vis)
-    - [--fusion_inspector](#--fusion_inspector)
-- [Reference genomes](#reference-genomes)
-    - [--arriba_ref](#--arriba_ref)
-    - [--databases](#--databases)
-    - [--ericscript_ref](#--ericscript_ref)
-    - [--fasta](#--fasta)
-    - [--fusioncatcher_ref](#--fusioncatcher_ref)
-    - [--genome](#--genome)
-    - [--gtf](#--gtf)
-    - [--star_index](#--star_index)
-    - [--star_fusion_ref](#--star_fusion_ref)
-    - [--transcript](#--transcript)
-- [Other command line parameters](#other-command-line-parameters)
-    - [--debug](#--debug)
-    - [--read_length](#--read_length)
-    - [--outdir](#--outdir)
-    - [--email](#--email)
-    - [--email_on_fail](#--email_on_fail)
-    - [--max_multiqc_email_size](#--max_multiqc_email_size)
-    - [-name](#-name)
-    - [--custom_config_version](#--custom_config_version)
-    - [--custom_config_base](#--custom_config_base)
-- [Job resources](#job-resources)
-    - [Automatic resubmission](#automatic-resubmission)
-
-## Download references
-
-The rnafusion pipeline needs references for the fusion detection tools, so downloading these is a prerequisite.
-
-Downloading references manually is a tedious long process.
-To make the pipeline easier to work with, we provide a script to download all necessary references for fusion detection tools.
-
-> **TL;DR:** Make sure to download the correct references for your need!
-
-### Download all references
-
-```bash
-nextflow run nf-core/rnafusion \
---build-references \
---outdir <PATH> \
-```
-
-### Example of downloading references for specific tool
+The pipeline is divided into two parts:
 
-```bash
-nextflow run nf-core/rnafusion/download-references.nf \
---arriba \
---outdir <PATH>
+1. Downloading and building the references, using `--build_references`: done only once and after each update.
+2. Detecting fusions using (any combination of) the following tools:
+    * arriba
+    * fusioncatcher
+    * pizzly
+    * squid
+    * starfusion
+3. QC and visualisation tools
+    * Fastqc
+    * MultiQC
+    * arriba visualisation (for fusion detected by arriba only)
+    * fusion-report
+    * fusionInspector
 
-nextflow run nf-core/rnafusion/download-references.nf \
---star_fusion \
---outdir <PATH>
+### Prerequisite: download and build references
 
-nextflow run nf-core/rnafusion/download-references.nf \
---fusioncatcher \
---outdir <PATH>
+The rnafusion pipeline needs references for the fusion detection tools, so downloading these is a **requirement**.
+It is possible to download and build each reference manually (for example in case of non-human samples that are not supported currently) feed references manually to rnafusion using the arguments `--<tool>_ref` but it is advised to download references with rnafusion:
 
-nextflow run nf-core/rnafusion/download-references.nf \
---ericscript \
---outdir <PATH>
-
-nextflow run nf-core/rnafusion/download-references.nf \
---fusion_report \
---outdir <PATH>
+```bash
+nextflow run nf-core/rnafusion \
+--build_references --all \
+--genomes_base <PATH> \
 ```
 
-## Download and build CTAT
-
+References for the different tools can also be downloaded separately:
 ```bash
-nextflow run nf-core/rnafusion/build-ctat.nf \
---genome GRCh38 \
---outdir <PATH> \
---fasta <PATH>/<FASTA \
---gtf <PATH>/<GTF>
+nextflow run nf-core/rnafusion \
+--build_references --<tool> \
+--genomes_base <PATH>
 ```
 
-## Download GRCh37 references
-
-```bash
-# GRCh38 genome assembly is used by default.
-# To use the previous assembly specify it using the --genome flag
-nextflow run nf-core/rnafusion/download-references.nf \
---genome GRCh37 \
---download_all \
---outdir <PATH> \
---cosmic_usr <COSMIC_USER> --cosmic_passwd <COSMIC_PASSWD>
+This PATH will be the place the references will be saved.
 
-# Please note that using the above example command downloads NCBI-based references for STAR-Fusion.
-# To use Ensembl-based references run the following command with the same <PATH> as used above
+### Running the detection tools
 
-nextflow run nf-core/rnafusion/build-ctat.nf \
---genome GRCh37 \
+```bash
+nextflow run nf-core/rnafusion \
+--build_references --all \
 --outdir <PATH> \
---fasta <PATH>/<FASTA> \
---gtf <PATH>/<GTF>
 ```
 
-### Tool reference requirements
+Visualisation tools will be run on all fusion detected.
 
-| Tool             |        FASTA       |         GTF        |     STAR-index     |     Genome     |       Other       |
-| ---------------- | :----------------: | :----------------: | :----------------: | :------------: | :----------------: |
-| Arriba           | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 | `custom_reference` |
-| EricScript       |         :x:        |         :x:        |         :x:        | GRCh37, GRCh38 | `custom_reference` |
-| FusionCatcher    |         :x:        |         :x:        |         :x:        |     GRCh38     | `custom_reference` |
-| Fusion-Inspector | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |  `ctat_genome_lib` |
-| fusion-report    |         :x:        |         :x:        |         :x:        | GRCh37, GRCh38 |     `databases`    |
-| Pizzly           |         :x:        | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |       `cDNA`       |
-| Squid            |         :x:        | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |          -         |
-| Star-Fusion      | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |  `ctat_genome_lib` |
+#### Optional manual feed-in of fusion files
 
+It is possible to give the output of each tool manually using the argument: `--<tool>_fusions PATH/TO/FUSION/FILE`.
 ## Samplesheet input
 
 You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below.
@@ -196,7 +103,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p
 The typical command for running the pipeline is as follows.
 
 ```console
-nextflow run nf-core/rnafusion --input samplesheet.csv --genome GRCh37 -profile docker
+nextflow run nf-core/rnafusion --input samplesheet.csv --genome GRCh38 -profile docker
 ```
 
 This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
@@ -245,21 +152,21 @@ They are loaded in sequence, so later profiles can overwrite earlier profiles.
 
 If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended.
 
-- `docker`
-    - A generic configuration profile to be used with [Docker](https://docker.com/)
-- `singularity`
-    - A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
-- `podman`
-    - A generic configuration profile to be used with [Podman](https://podman.io/)
-- `shifter`
-    - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
-- `charliecloud`
-    - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
-- `conda`
-    - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
-- `test`
-    - A profile with a complete configuration for automated testing
-    - Includes links to test data so needs no other parameters
+* `docker`
+    * A generic configuration profile to be used with [Docker](https://docker.com/)
+* `singularity`
+    * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
+* `podman`
+    * A generic configuration profile to be used with [Podman](https://podman.io/)
+* `shifter`
+    * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
+* `charliecloud`
+    * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
+* `conda`
+    * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
+* `test`
+    * A profile with a complete configuration for automated testing
+    * Includes links to test data so needs no other parameters
 
 ### `-resume`
 
@@ -280,11 +187,11 @@ Whilst the default requirements set within the pipeline will hopefully work for
 For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue:
 
 ```console
-[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
-Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
+[62/149eb0] NOTE: Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
+Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
 
 Caused by:
-    Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
+    Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
 
 Command executed:
     STAR \
@@ -308,55 +215,24 @@ Work dir:
 Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
 ```
 
-To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
+To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN).
+We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/software/star/align/main.nf`.
+If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9).
+The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements.
+The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB.
+Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB.
+The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
 
 ```nextflow
 process {
-    withName: STAR_ALIGN {
+    withName: 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN' {
         memory = 100.GB
     }
 }
 ```
 
-> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow.
-
-<!-- TODO: adapt to current DSL2 parameters -->
-### Tool-specific options
-
-For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes.
-
-The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of:
-
-- Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file.
-
-- An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option.
-
-The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments.
-
-When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`.
-
-As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file.
-
-Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config.
-
-As you will see in the example below, we have:
-
-- appended `--outFilterMismatchNmax 16` to the default `args` used by the module.
-- changed the default `publishDir` value to where the files will eventually be published in the main results directory.
-- appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`.
-
-```nextflow
-params {
-    modules {
-        'star_align' {
-            args          = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16"
-            publishDir    = "my_star_directory"
-            publish_files = ['out':'log', 'tab':'log', 'bam':'']
-        }
-    }
-}
-```
-
+> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden.
+> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
 ### Updating containers
 
 The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`.
@@ -365,7 +241,7 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
 2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
 3. Create the custom config accordingly:
 
-    - For Docker:
+    * For Docker:
 
         ```nextflow
         process {
@@ -375,7 +251,7 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
         }
         ```
 
-    - For Singularity:
+    * For Singularity:
 
         ```nextflow
         process {
@@ -385,7 +261,7 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
         }
         ```
 
-    - For Conda:
+    * For Conda:
 
         ```nextflow
         process {
@@ -403,7 +279,7 @@ In most cases, you will only need to create a custom config as a one-off but if
 
 See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files.
 
-If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs).
+If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). -->
 
 ## Running in the background
 
@@ -422,238 +298,3 @@ We recommend adding the following line to your environment to limit this (typica
 ```console
 NXF_OPTS='-Xms1g -Xmx4g'
 ```
-
-## Pipeline specific arguments
-
-### --input
-
-Use this to specify the location of your input FastQ files. For example:
-
-```bash
---input 'path/to/data/sample_*_{1,2}.fastq.gz'
-```
-
-Please note the following requirements:
-
-1. The path must be enclosed in quotes
-2. The path must have at least one `*` wildcard character
-3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.
-
-If left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`
-
-### --single_end
-
-By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:
-
-```bash
---single_end --input '*.fastq'
-```
-
-### Tool flags
-
-### --arriba
-
-If enabled, executes `Arriba` tool.
-
-- `--arriba_opt`
-    - Specify additional parameters. For more information, please refer to the [documentation](http://arriba.readthedocs.io/en/latest/quickstart/) of the tool.
-
-### --ericscript
-
-If enabled, executes `Ericscript` tool.
-
-- `--ericscript_opt`
-    - Specify additional parameters. For more information, please refer to the [documentation](https://sites.google.com/site/bioericscript/home) of the tool.
-
-### --fusioncatcher
-
-If enabled, executes `Fusioncatcher` tool.
-
-> N.B. that Fusioncatcher is not available when using the `GRCh37` genome assembly.
-
-- `--fusioncatcher_opt`
-    - Specify additional parameters. For more information, please refer to the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md) of the tool.
-
-### --fusion_report
-
-If enabled, download databases for `fusion-report`.
-
-- `fusion_report_opt`
-    - Specify additional parameters. For more information, please refer to the [documentation](https://matq007.github.io/fusion-report/#/) of the tool.
-
-### --pizzly
-
-If enabled, executes `Pizzly` tool.
-
-- `--pizzly_k`
-    - Number of k-mers. Default `31`.
-
-### --squid
-
-If enabled, executes `Squid` tool.
-
-### --star_fusion
-
-If enabled, executes `STAR-Fusion` tool.
-
-- `--star_fusion_opt`
-    - Parameter for specifying additional parameters. For more information, please refer to the [documentation](https://github.com/STAR-Fusion/STAR-Fusion/wiki) of the tool.
-
-## Visualization flags
-
-### --arriba_vis
-
-If enabled, executes build in `Arriba` visualization tool.
-
-### --fusion_inspector
-
-If enabled, executes `Fusion-Inspector` tool.
-
-## Reference genomes
-
-### --arriba_ref
-
-```bash
---arriba_ref '<path to Arriba reference>'
-```
-
-### --databases
-
-Required databases in order to run `fusion-report`.
-
-```bash
---databases '<path to fusion-report databases>'
-```
-
-### --ericscript_ref
-
-Required reference in order to run `EricScript`.
-
-```bash
---ericscript_ref '<path to EricScript reference>'
-```
-
-### --fasta
-
-If you prefer, you can specify the full path to your reference genome when you run the pipeline:
-
-```bash
---fasta '<path to Fasta reference>'
-```
-
-### --fusioncatcher_ref
-
-Required reference in order to run `Fusioncatcher`.
-
-```bash
---fusioncatcher_ref '<path to Fusioncatcher reference>'
-```
-
-### --genome
-
-This pipeline uses `Homo Sapiens` version `GRCh38` by default. Assembly `GRCh37` is optionally available.
-
-> N.B. that using `GRCh37` precludes use of the `Fusioncatcher` tool.
-> Also make sure to specify `--genomes_base`.
-
-```bash
---genome 'GRCh38' --genome_base '</path/to/references>'
-```
-
-### --gtf
-
-Required annotation file.
-
-```bash
---gtf '<path to GTF annotation>'
-```
-
-### --star_index
-
-If you prefer, you can specify the full path for `STAR` index when you run the pipeline.
-If not specified, the pipeline will build the index using for reads with length `100bp` (can be adjusted with parameter `--read_length`).
-
-```bash
---star_index '<path to STAR index>'
-```
-
-### --star_fusion_ref
-
-Required reference in order to run `STAR-Fusion`.
-
-```bash
---star_fusion_ref '<path to STAR-Fusion reference>'
-```
-
-### --transcript
-
-Required transcript file.
-
-```bash
---transcript '<path to transcript reference>'
-```
-
-## Other command line parameters
-
-### --debug
-
-To run only a specific tool (testing freshly implemented tool) just add `--debug` parameter.
-This parameter only works on **fusion tools only**!
-
-### --read_length
-
-Length is used to build a STAR index.
-Default is `100bp` (Illumina).
-
-### --outdir
-
-The output directory where the results will be saved.
-
-### --email
-
-Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits.
-If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.
-
-### --email_on_fail
-
-This works exactly as with `--email`, except emails are only sent if the workflow is not successful.
-
-### --max_multiqc_email_size
-
-Threshold size for MultiQC report to be attached in notification email.
-If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB).
-
-### -name
-
-Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic.
-This is used in the MultiQC report (if not default) and in the summary HTML / e-mail (always).
-
-**NB:** Single hyphen (core Nextflow option)
-
-### --custom_config_version
-
-Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.
-
-```bash
-## Download and use config file with following git commid id
---custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96
-```
-
-### --custom_config_base
-
-If you're running offline, nextflow will not be able to fetch the institutional config files
-from the internet. If you don't need them, then this is not a problem. If you do need them,
-you should download the files from the repo and tell nextflow where to find them with the
-`custom_config_base` option. For example:
-
-```bash
-NXF_OPTS='-Xms1g -Xmx4g'
-```
-
-## Job resources
-
-### Automatic resubmission
-
-Each step in the pipeline has a default set of requirements for number of CPUs, memory and time.
-For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original).
-If it still fails after three times then the pipeline is stopped.
diff --git a/modules/local/fusioninspector/meta.yml b/modules/local/fusioninspector/meta.yml
index 5002fe28..98f86a1c 100644
--- a/modules/local/fusioninspector/meta.yml
+++ b/modules/local/fusioninspector/meta.yml
@@ -26,7 +26,6 @@ input:
       description: Path to ctat references
       pattern: "*"
 
-# TODO complete output
 output:
   - versions:
         type: file
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index ae51f880..746c3465 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -17,9 +17,6 @@ process FUSIONREPORT_DOWNLOAD {
     path "*"                , emit: reference
     path "versions.yml"     , emit: versions
 
-
-    //TODO: update mitelman to take DB from https://storage.googleapis.com/mitelman-data-files/prod/mitelman_db.zip
-    //TODO: update fusionGDB to GRCh38
     script:
     """
     fusion_report download --cosmic_usr $username --cosmic_passwd $passwd .
diff --git a/nextflow.config b/nextflow.config
index 14be0540..98ea6359 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -24,7 +24,7 @@ params {
     // Genome
     genome                     = 'GRCh38'
     genomes_base               = null
-    ensembl_version            = 97
+    ensembl_version            = 105
     read_length                = 100
     genomes                    = [:]
 
@@ -37,7 +37,6 @@ params {
     // Enable or disable tools
     all                        = false
     arriba                     = false
-    ericscript                 = false
     fusioncatcher              = false
     pizzly                     = false
     squid                      = false
@@ -48,7 +47,6 @@ params {
     // Path to references
     arriba_ref                    = "${params.genomes_base}/arriba"
     ensembl_ref                   = "${params.genomes_base}/ensembl"
-    ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v102"
     pizzly_ref                    = "${params.genomes_base}/pizzly"
     squid_ref                     = "${params.genomes_base}/squid"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 371b773a..0aadc869 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -56,25 +56,23 @@
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
                     "description": "ensembl version",
-                    "default": "97"
+                    "default": 105
                 },
                 "read_length": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
                     "description": "Read length",
-                    "default": "100"
+                    "default": 100
                 },
                 "all": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run all references/analyses",
-                    "default": "false"
+                    "description": "Build or run all references/analyses"
                 },
                 "arriba": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run arriba references/analyses",
-                    "default": "false"
+                    "description": "Build or run arriba references/analyses"
                 },
                 "arriba_ref": {
                     "type": "string",
@@ -91,22 +89,10 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to ensembl references"
                 },
-                "ericscript": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Build or run ericscript references/analyses",
-                    "default": "false"
-                },
-                "ericscript_ref": {
-                    "type": "string",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to ericscript references"
-                },
                 "fusioncatcher": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run fusioncatcher references/analyses",
-                    "default": "false"
+                    "description": "Build or run fusioncatcher references/analyses"
                 },
                 "fusioncatcher_fusions": {
                     "type": "string",
@@ -121,8 +107,7 @@
                 "fusionreport": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build fusionreport references",
-                    "default": "false"
+                    "description": "Build fusionreport references"
                 },
                 "fusionreport_ref": {
                     "type": "string",
@@ -132,8 +117,7 @@
                 "pizzly": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run pizzly references/analyses",
-                    "default": "false"
+                    "description": "Build or run pizzly references/analyses"
                 },
                 "pizzly_fusions": {
                     "type": "string",
@@ -148,8 +132,7 @@
                 "squid": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run squid references/analyses",
-                    "default": "false"
+                    "description": "Build or run squid references/analyses"
                 },
                 "squid_fusions": {
                     "type": "string",
@@ -164,8 +147,7 @@
                 "starfusion": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run starfusion references/analyses",
-                    "default": "false"
+                    "description": "Build or run starfusion references/analyses"
                 },
                 "starfusion_fusions": {
                     "type": "string",
@@ -180,8 +162,7 @@
                 "starindex": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run starindex references/analyses",
-                    "default": "false"
+                    "description": "Build or run starindex references/analyses"
                 },
                 "starindex_ref": {
                     "type": "string",
@@ -428,4 +409,4 @@
             "$ref": "#/definitions/generic_options"
         }
     ]
-}
+}
\ No newline at end of file
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 16b52419..ddd1a139 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -21,7 +21,7 @@ workflow ARRIBA_WORKFLOW {
         ch_versions = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
-        if (params.arriba) {
+        if (params.arriba || params.all) {
             gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
             star_ignore_sjdbgtf = false
             seq_platform = false
diff --git a/subworkflows/local/fusioncatcher_workflow.nf b/subworkflows/local/fusioncatcher_workflow.nf
index 28201875..f664d6b1 100644
--- a/subworkflows/local/fusioncatcher_workflow.nf
+++ b/subworkflows/local/fusioncatcher_workflow.nf
@@ -14,7 +14,7 @@ workflow FUSIONCATCHER_WORKFLOW {
         ch_versions = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_fusioncatcher.txt", checkIfExists: true)
 
-        if (params.fusioncatcher) {
+        if (params.fusioncatcher || params.all) {
             if (params.fusioncatcher_fusions){
                 ch_fusioncatcher_fusions = params.fusioncatcher_fusions
             } else {
diff --git a/subworkflows/local/pizzly_workflow.nf b/subworkflows/local/pizzly_workflow.nf
index 52bdd44b..40b51dda 100644
--- a/subworkflows/local/pizzly_workflow.nf
+++ b/subworkflows/local/pizzly_workflow.nf
@@ -15,7 +15,7 @@ workflow PIZZLY_WORKFLOW {
         ch_versions = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_pizzly.txt", checkIfExists: true)
 
-        if (params.pizzly) {
+        if (params.pizzly || params.all) {
             if (params.pizzly_fusions) {
                 ch_pizzly_fusions = params.pizzly_fusions
             } else {
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index be2a485f..c22d45e7 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -18,7 +18,7 @@ workflow SQUID_WORKFLOW {
         ch_versions = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_squid.txt", checkIfExists: true)
 
-        if (params.squid) {
+        if (params.squid || params.all) {
             if (params.squid_fusions){
                 ch_squid_fusions = params.squid_fusions
             } else {
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index 568df66e..19ad7cba 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -15,7 +15,7 @@ workflow STARFUSION_WORKFLOW {
         ch_versions = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_starfusion.txt", checkIfExists: true)
 
-        if (params.starfusion){
+        if (params.starfusion || params.all){
             if (params.starfusion_fusions){
                 ch_starfusion_fusions = params.starfusion_fusions
             } else {
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index ad9c3f33..cf651788 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -6,7 +6,6 @@
 
 include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'
 include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'
-include { ERICSCRIPT_DOWNLOAD }             from '../modules/local/ericscript/download/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/download/main'
 include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
@@ -38,10 +37,6 @@ workflow BUILD_REFERENCES {
         ARRIBA_DOWNLOAD()
     }
 
-    if (params.ericscript || params.all) {
-        ERICSCRIPT_DOWNLOAD()
-    }
-
     if (params.fusioncatcher || params.all) {
         FUSIONCATCHER_DOWNLOAD()
     }
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index ca1ae5a9..4d400ef7 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -18,7 +18,7 @@ def checkPathParamList = [
     params.fasta, params.genomes_base,
     params.ensembl_ref,
     params.fusioncatcher_ref, params.starfusion_ref,
-    params.arriba_ref, params.ericscript_ref,
+    params.arriba_ref,
     params.ensembl_version
 ]
 

From a3a09b8c95716fdf8f8369f49d9fb65dba2a5b60 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:41:37 +0100
Subject: [PATCH 203/359] add strandedness in samplesheet

---
 bin/check_samplesheet.py | 46 +++++++++++++++++++++++++++++++---------
 1 file changed, 36 insertions(+), 10 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 646150dc..8d381682 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -42,10 +42,9 @@ def check_samplesheet(file_in, file_out):
     """
     This function checks that the samplesheet follows the following structure:
 
-    sample,fastq_1,fastq_2
-    SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz
-    SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz
-    SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,
+    sample,fastq_1,fastq_2,strandedness
+    SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,forward
+    SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,forward
 
     For an example see:
     https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
@@ -56,7 +55,7 @@ def check_samplesheet(file_in, file_out):
 
         ## Check header
         MIN_COLS = 2
-        HEADER = ["sample", "fastq_1", "fastq_2"]
+        HEADER = ["sample", "fastq_1", "fastq_2", "strandedness"]
         header = [x.strip('"') for x in fin.readline().strip().split(",")]
         if header[: len(HEADER)] != HEADER:
             print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
@@ -82,7 +81,7 @@ def check_samplesheet(file_in, file_out):
                 )
 
             ## Check sample name entries
-            sample, fastq_1, fastq_2 = lspl[: len(HEADER)]
+            sample, fastq_1, fastq_2 , strandedness = lspl[: len(HEADER)]
             sample = sample.replace(" ", "_")
             if not sample:
                 print_error("Sample entry has not been specified!", "Line", line)
@@ -99,16 +98,32 @@ def check_samplesheet(file_in, file_out):
                             line,
                         )
 
+            ## Check strandedness
+            strandednesses = ["unstranded", "forward", "reverse"]
+            if strandedness:
+                if strandedness not in strandednesses:
+                    print_error(
+                        f"Strandedness must be one of '{', '.join(strandednesses)}'!",
+                        "Line",
+                        line,
+                    )
+            else:
+                print_error(
+                    f"Strandedness has not been specified! Must be one of {', '.join(strandednesses)}.",
+                    "Line",
+                    line,
+                )
+
             ## Auto-detect paired-end/single-end
-            sample_info = []  ## [single_end, fastq_1, fastq_2]
+            sample_info = []  ## [single_end, fastq_1, fastq_2, strandedness]
             if sample and fastq_1 and fastq_2:  ## Paired-end short reads
-                sample_info = ["0", fastq_1, fastq_2]
+                sample_info = ["0", fastq_1, fastq_2, strandedness]
             elif sample and fastq_1 and not fastq_2:  ## Single-end short reads
-                sample_info = ["1", fastq_1, fastq_2]
+                sample_info = ["1", fastq_1, fastq_2, strandedness]
             else:
                 print_error("Invalid combination of columns provided!", "Line", line)
 
-            ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] }
+            ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2, strandedness ] }
             if sample not in sample_mapping_dict:
                 sample_mapping_dict[sample] = [sample_info]
             else:
@@ -129,6 +144,17 @@ def check_samplesheet(file_in, file_out):
                 if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]):
                     print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample))
 
+                ## Check that multiple runs of the same sample are of the same strandedness
+                if not all(
+                    x[-1] == sample_mapping_dict[sample][0][-1]
+                    for x in sample_mapping_dict[sample]
+                ):
+                    print_error(
+                        f"Multiple runs of a sample must have the same strandedness!",
+                        "Sample",
+                        sample,
+                    )
+
                 for idx, val in enumerate(sample_mapping_dict[sample]):
                     fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n")
     else:

From 11dff2a5ccbd7bb1c251eacaf69be16e4e451928 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:42:30 +0100
Subject: [PATCH 204/359] position of fasta transcript and gtf moved into
 ensembl_ref

---
 conf/genomes.config | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index 5bdd6d58..92c07a6e 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -11,9 +11,9 @@
 params {
     genomes {
         'GRCh38' {
-            fasta                         = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
-            gtf                           = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-            transcript                    = "${params.genomes_base}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
+            fasta                         = "${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
+            gtf                           = "${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+            transcript                    = "${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
             arriba_ref                    = "${params.genomes_base}/arriba"
             ensembl_ref                   = "${params.genomes_base}/ensembl"
             ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"

From 28d016bd2fa59a16b0aa84aaea84397f8ec15fc7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:44:08 +0100
Subject: [PATCH 205/359] install nf-core modules picard/collectwgsmetrics,
 qualimap/rnaseq and salmon/index

---
 modules.json                                  |  9 ++++
 .../picard/collctrnaseqmetrics/main.nf        | 36 +++++++++++++
 .../picard/collctrnaseqmetrics/meta.yml       | 49 +++++++++++++++++
 .../modules/picard/collectwgsmetrics/main.nf  | 44 ++++++++++++++++
 .../modules/picard/collectwgsmetrics/meta.yml | 47 +++++++++++++++++
 .../nf-core/modules/qualimap/rnaseq/main.nf   | 52 +++++++++++++++++++
 modules/nf-core/modules/salmon/index/main.nf  | 46 ++++++++++++++++
 modules/nf-core/modules/salmon/index/meta.yml | 36 +++++++++++++
 8 files changed, 319 insertions(+)
 create mode 100644 modules/nf-core/modules/picard/collctrnaseqmetrics/main.nf
 create mode 100644 modules/nf-core/modules/picard/collctrnaseqmetrics/meta.yml
 create mode 100644 modules/nf-core/modules/picard/collectwgsmetrics/main.nf
 create mode 100644 modules/nf-core/modules/picard/collectwgsmetrics/meta.yml
 create mode 100644 modules/nf-core/modules/qualimap/rnaseq/main.nf
 create mode 100644 modules/nf-core/modules/salmon/index/main.nf
 create mode 100644 modules/nf-core/modules/salmon/index/meta.yml

diff --git a/modules.json b/modules.json
index b7ee22d9..07b979de 100644
--- a/modules.json
+++ b/modules.json
@@ -21,6 +21,15 @@
             "multiqc": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
+            "picard/collectwgsmetrics": {
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+            },
+            "qualimap/rnaseq": {
+                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+            },
+            "salmon/index": {
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+            },
             "samtools/faidx": {
                 "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
             },
diff --git a/modules/nf-core/modules/picard/collctrnaseqmetrics/main.nf b/modules/nf-core/modules/picard/collctrnaseqmetrics/main.nf
new file mode 100644
index 00000000..dbbc7198
--- /dev/null
+++ b/modules/nf-core/modules/picard/collctrnaseqmetrics/main.nf
@@ -0,0 +1,36 @@
+process PICARD_COLLECTRNASEQMETRICS{
+
+    input:
+        tuple val(sample), path(bam), path(bai)
+        path(refflat)
+        path(rrna_intervals)
+
+    output:
+        tuple val(sample), path("${sample}_rna_metrics.txt"), emit metrics
+
+    script:
+    def strandedness = ''
+    if (params.strandedness == 'forward') {
+        strandedness = '--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND'
+    } else if (params.strandedness == 'reverse') {
+        strandedness = '--STRAND_SPECIFICITY SECOND_READ_TRANSCRIPTION_STRAND'
+    }
+    def rrna = rrna_intervals == [] ? '' : "--RIBOSOMAL_INTERVALS ${rrna_intervals}"
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def avail_mem = 3
+    if (!task.memory) {
+        log.info '[Picard CollectWgsMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
+    } else {
+        avail_mem = task.memory.giga
+    }
+    """
+    picard CollectRnaSeqMetrics \\
+        --TMP_DIR ./tmp \\
+        ${strandedness} \\
+        ${rrna} \\
+        --REF_FLAT ${refflat} \\
+        --INPUT ${bam} \\
+        --OUTPUT ${prefix}_rna_metrics.txt \\
+    """
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/picard/collctrnaseqmetrics/meta.yml b/modules/nf-core/modules/picard/collctrnaseqmetrics/meta.yml
new file mode 100644
index 00000000..75541034
--- /dev/null
+++ b/modules/nf-core/modules/picard/collctrnaseqmetrics/meta.yml
@@ -0,0 +1,49 @@
+name: picard_collectrnaseqmetrics
+description: Produces RNA alignment metrics for a SAM or BAM file. 
+keywords:
+  - alignment
+  - metrics
+  - statistics
+  - quality
+  - bam
+  - RNA
+tools:
+  - picard:
+      description: |
+        A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS)
+        data and formats such as SAM/BAM/CRAM and VCF.
+      homepage: https://broadinstitute.github.io/picard/
+      documentation: https://broadinstitute.github.io/picard/
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM file
+      pattern: "*.{bam}"
+  - refflat:
+      type: file
+      description: Gene annotations in refFlat form
+  - rrna_interval:
+      type: file
+      description: Location of rRNA sequences in genome, in interval_list format
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - metrics:
+      type: file
+      description: Alignment metrics files generated by picard
+      pattern: "*_{metrics}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@rannick"
diff --git a/modules/nf-core/modules/picard/collectwgsmetrics/main.nf b/modules/nf-core/modules/picard/collectwgsmetrics/main.nf
new file mode 100644
index 00000000..f4efaa4c
--- /dev/null
+++ b/modules/nf-core/modules/picard/collectwgsmetrics/main.nf
@@ -0,0 +1,44 @@
+process PICARD_COLLECTWGSMETRICS {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
+        'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+    path  fasta
+
+    output:
+    tuple val(meta), path("*_metrics"), emit: metrics
+    path  "versions.yml"              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def avail_mem = 3
+    if (!task.memory) {
+        log.info '[Picard CollectWgsMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
+    } else {
+        avail_mem = task.memory.giga
+    }
+    """
+    picard \\
+        -Xmx${avail_mem}g \\
+        CollectWgsMetrics \\
+        $args \\
+        INPUT=$bam \\
+        OUTPUT=${prefix}.CollectWgsMetrics.coverage_metrics \\
+        REFERENCE_SEQUENCE=$fasta
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        picard: \$(picard CollectWgsMetrics --version 2>&1 | grep -o 'Version.*' | cut -f2- -d:)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/picard/collectwgsmetrics/meta.yml b/modules/nf-core/modules/picard/collectwgsmetrics/meta.yml
new file mode 100644
index 00000000..d6c3d012
--- /dev/null
+++ b/modules/nf-core/modules/picard/collectwgsmetrics/meta.yml
@@ -0,0 +1,47 @@
+name: picard_collectwgsmetrics
+description: Collect metrics about coverage and performance of whole genome sequencing (WGS) experiments.
+keywords:
+  - alignment
+  - metrics
+  - statistics
+  - quality
+  - bam
+tools:
+  - picard:
+      description: |
+        A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS)
+        data and formats such as SAM/BAM/CRAM and VCF.
+      homepage: https://broadinstitute.github.io/picard/
+      documentation: https://broadinstitute.github.io/picard/
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM file
+      pattern: "*.{bam}"
+  - fasta:
+      type: file
+      description: Genome fasta file
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - metrics:
+      type: file
+      description: Alignment metrics files generated by picard
+      pattern: "*_{metrics}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
+  - "@flowuenne"
+  - "@lassefolkersen"
diff --git a/modules/nf-core/modules/qualimap/rnaseq/main.nf b/modules/nf-core/modules/qualimap/rnaseq/main.nf
new file mode 100644
index 00000000..3b2f88ad
--- /dev/null
+++ b/modules/nf-core/modules/qualimap/rnaseq/main.nf
@@ -0,0 +1,52 @@
+process QUALIMAP_RNASEQ {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::qualimap=2.2.2d" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/qualimap:2.2.2d--1' :
+        'quay.io/biocontainers/qualimap:2.2.2d--1' }"
+
+    input:
+    tuple val(meta), path(bam)
+    path  gtf
+
+    output:
+    tuple val(meta), path("${prefix}"), emit: results
+    path  "versions.yml"              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args   ?: ''
+    prefix   = task.ext.prefix ?: "${meta.id}"
+    def paired_end = meta.single_end ? '' : '-pe'
+    def memory     = task.memory.toGiga() + "G"
+
+    def strandedness = 'non-strand-specific'
+    if (meta.strandedness == 'forward') {
+        strandedness = 'strand-specific-forward'
+    } else if (meta.strandedness == 'reverse') {
+        strandedness = 'strand-specific-reverse'
+    }
+    """
+    unset DISPLAY
+    mkdir tmp
+    export _JAVA_OPTIONS=-Djava.io.tmpdir=./tmp
+    qualimap \\
+        --java-mem-size=$memory \\
+        rnaseq \\
+        $args \\
+        -bam $bam \\
+        -gtf $gtf \\
+        -p $strandedness \\
+        $paired_end \\
+        -outdir $prefix
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        qualimap: \$(echo \$(qualimap 2>&1) | sed 's/^.*QualiMap v.//; s/Built.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/salmon/index/main.nf b/modules/nf-core/modules/salmon/index/main.nf
new file mode 100644
index 00000000..737087f9
--- /dev/null
+++ b/modules/nf-core/modules/salmon/index/main.nf
@@ -0,0 +1,46 @@
+process SALMON_INDEX {
+    tag "$transcript_fasta"
+    label "process_medium"
+
+    conda (params.enable_conda ? 'bioconda::salmon=1.5.2' : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/salmon:1.5.2--h84f40af_0' :
+        'quay.io/biocontainers/salmon:1.5.2--h84f40af_0' }"
+
+    input:
+    path genome_fasta
+    path transcript_fasta
+
+    output:
+    path "salmon"       , emit: index
+    path "versions.yml" , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def get_decoy_ids = "grep '^>' $genome_fasta | cut -d ' ' -f 1 > decoys.txt"
+    def gentrome      = "gentrome.fa"
+    if (genome_fasta.endsWith('.gz')) {
+        get_decoy_ids = "grep '^>' <(gunzip -c $genome_fasta) | cut -d ' ' -f 1 > decoys.txt"
+        gentrome      = "gentrome.fa.gz"
+    }
+    """
+    $get_decoy_ids
+    sed -i.bak -e 's/>//g' decoys.txt
+    cat $transcript_fasta $genome_fasta > $gentrome
+
+    salmon \\
+        index \\
+        --threads $task.cpus \\
+        -t $gentrome \\
+        -d decoys.txt \\
+        $args \\
+        -i salmon
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        salmon: \$(echo \$(salmon --version) | sed -e "s/salmon //g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/salmon/index/meta.yml b/modules/nf-core/modules/salmon/index/meta.yml
new file mode 100644
index 00000000..53c64152
--- /dev/null
+++ b/modules/nf-core/modules/salmon/index/meta.yml
@@ -0,0 +1,36 @@
+name: salmon_index
+description: Create index for salmon
+keywords:
+  - index
+  - fasta
+  - genome
+  - reference
+tools:
+  - salmon:
+      description: |
+        Salmon is a tool for wicked-fast transcript quantification from RNA-seq data
+      homepage: https://salmon.readthedocs.io/en/latest/salmon.html
+      manual: https://salmon.readthedocs.io/en/latest/salmon.html
+      doi: 10.1038/nmeth.4197
+      licence: ["GPL-3.0-or-later"]
+input:
+  - genome_fasta:
+      type: file
+      description: Fasta file of the reference genome
+  - transcriptome_fasta:
+      type: file
+      description: Fasta file of the reference transcriptome
+
+output:
+  - index:
+      type: directory
+      description: Folder containing the star index files
+      pattern: "salmon"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@kevinmenden"
+  - "@drpatelh"

From 0788eab9dd37691f5e7195a989165796d1e5656d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:45:02 +0100
Subject: [PATCH 206/359] remove default ensembl value from nextflow.json

---
 nextflow_schema.json | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 371b773a..020ded1c 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -55,8 +55,7 @@
                 "ensembl_version": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
-                    "description": "ensembl version",
-                    "default": "97"
+                    "description": "ensembl version"
                 },
                 "read_length": {
                     "type": "integer",

From b18a682dd7585b8274358a3acc910b5fb8d3e298 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:46:54 +0100
Subject: [PATCH 207/359] add workflow specific alias to samtools view and sort

---
 subworkflows/local/arriba_workflow.nf | 8 ++++----
 subworkflows/local/squid_workflow.nf  | 8 ++++----
 2 files changed, 8 insertions(+), 8 deletions(-)

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 16b52419..8c78e28a 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -7,7 +7,7 @@ include { ARRIBA_VISUALISATION }                        from '../../modules/loca
 include { GET_PATH as GET_PATH_ARRIBA }                 from '../../modules/local/getpath/main'
 include { GET_PATH as GET_PATH_ARRIBA_FAIL }            from '../../modules/local/getpath/main'
 include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_ARRIBA }   from '../../modules/nf-core/modules/samtools/sort/main'
-include { SAMTOOLS_INDEX }                              from '../../modules/nf-core/modules/samtools/index/main'
+include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_ARRIBA}  from '../../modules/nf-core/modules/samtools/index/main'
 include { STAR_ALIGN as STAR_FOR_ARRIBA }               from '../../modules/nf-core/modules/star/align/main'
 
 
@@ -33,10 +33,10 @@ workflow ARRIBA_WORKFLOW {
             SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam)
             ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_ARRIBA.out.versions)
 
-            SAMTOOLS_INDEX(SAMTOOLS_SORT_FOR_ARRIBA.out.bam)
-            ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions)
+            SAMTOOLS_INDEX_FOR_ARRIBA(SAMTOOLS_SORT_FOR_ARRIBA.out.bam)
+            ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_ARRIBA.out.versions)
 
-            bam_indexed = SAMTOOLS_SORT_FOR_ARRIBA.out.bam.join(SAMTOOLS_INDEX.out.bai)
+            bam_indexed = SAMTOOLS_SORT_FOR_ARRIBA.out.bam.join(SAMTOOLS_INDEX_FOR_ARRIBA.out.bai)
 
             if (params.arriba_fusions) {
                 ch_arriba_fusions = params.arriba_fusions
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 6814c418..be2a485f 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -32,12 +32,12 @@ workflow SQUID_WORKFLOW {
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
             SAMTOOLS_VIEW_FOR_SQUID ( STAR_FOR_SQUID.out.sam, [] )
-            ch_versions = ch_versions.mix(SAMTOOLS_VIEW.out.versions )
+            ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_SQUID.out.versions )
 
-            SAMTOOLS_SORT_FOR_SQUID ( SAMTOOLS_VIEW.out.bam )
-            ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions )
+            SAMTOOLS_SORT_FOR_SQUID ( SAMTOOLS_VIEW_FOR_SQUID.out.bam )
+            ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_SQUID.out.versions )
 
-            bam_sorted = STAR_FOR_SQUID.out.bam_sorted.join(SAMTOOLS_SORT.out.bam )
+            bam_sorted = STAR_FOR_SQUID.out.bam_sorted.join(SAMTOOLS_SORT_FOR_SQUID.out.bam )
 
             SQUID ( bam_sorted )
             ch_versions = ch_versions.mix(SQUID.out.versions)

From 4fadeb6b617bf5b63e2f551b294eaa773d8aec7e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:49:54 +0100
Subject: [PATCH 208/359] emit bam from starfusion

---
 subworkflows/local/starfusion_workflow.nf | 6 +++++-
 1 file changed, 5 insertions(+), 1 deletion(-)

diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index 568df66e..837a7979 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -4,7 +4,7 @@
 
 include { STAR_ALIGN as STAR_FOR_STARFUSION }    from '../../modules/nf-core/modules/star/align/main'
 include { STARFUSION }                           from '../../modules/local/starfusion/detect/main'
-include { GET_PATH }                         from '../../modules/local/getpath/main'
+include { GET_PATH }                             from '../../modules/local/getpath/main'
 
 
 workflow STARFUSION_WORKFLOW {
@@ -13,6 +13,7 @@ workflow STARFUSION_WORKFLOW {
 
     main:
         ch_versions = Channel.empty()
+        ch_align = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_starfusion.txt", checkIfExists: true)
 
         if (params.starfusion){
@@ -28,6 +29,8 @@ workflow STARFUSION_WORKFLOW {
 
                 STAR_FOR_STARFUSION( reads, params.starindex_ref, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
+                ch_align = STAR_FOR_STARFUSION.out.bam
+
                 reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
 
                 STARFUSION( reads_junction, ref)
@@ -42,6 +45,7 @@ workflow STARFUSION_WORKFLOW {
         }
     emit:
         fusions         = ch_starfusion_fusions
+        bam             = ch_align.ifEmpty(null)
         versions        = ch_versions.ifEmpty(null)
 
 

From bbec348f4c0021e7925aade940a65db5e8db55cc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:50:31 +0100
Subject: [PATCH 209/359] bumb to ensembl 105

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 14be0540..9d1c283d 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -24,7 +24,7 @@ params {
     // Genome
     genome                     = 'GRCh38'
     genomes_base               = null
-    ensembl_version            = 97
+    ensembl_version            = 105
     read_length                = 100
     genomes                    = [:]
 

From c262031ccedc1a4cf06852cae316a70477fb72fb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:51:47 +0100
Subject: [PATCH 210/359] publish samtools index into different dirs by alias

---
 conf/modules.config | 16 ++++++++++++++++
 1 file changed, 16 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index 65ce1b06..b8e646f9 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -131,6 +131,22 @@ process {
         ]
     }
 
+    withName: SAMTOOLS_INDEX_FOR_ARRIBA {
+        publishDir = [
+            path: { "${params.outdir}/samtools_index_for_arriba" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
+    withName: SAMTOOLS_INDEX_FOR_QC {
+        publishDir = [
+            path: { "${params.outdir}/samtools_index_for_qc" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
     withName: SAMTOOLS_SORT_FOR_ARRIBA {
         ext.prefix = { "${meta.id}_sorted" }
         publishDir = [

From 6921f23ee04fa83728c47a53be5b9860874a9d04 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:52:46 +0100
Subject: [PATCH 211/359] draft of qc workflow

---
 subworkflows/local/qc_workflow.nf | 37 +++++++++++++++++++++++++++++++
 workflows/rnafusion.nf            | 13 +++++++++--
 2 files changed, 48 insertions(+), 2 deletions(-)
 create mode 100644 subworkflows/local/qc_workflow.nf

diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
new file mode 100644
index 00000000..a453d136
--- /dev/null
+++ b/subworkflows/local/qc_workflow.nf
@@ -0,0 +1,37 @@
+//
+// Check input samplesheet and get read channels
+//
+
+include { QUALIMAP_RNASEQ }    from '../../modules/nf-core/modules/qualimap/rnaseq/main'
+include { GTF_TO_REFFLAT }     from '../../modules/local/uscs/gtftogenepred/main'
+
+
+workflow QC_WORKFLOW {
+    take:
+        bam
+
+    main:
+        ch_versions = Channel.empty()
+        ch_qualimap_qc = Channel.empty()
+
+        if (params.starfusion && params.qc){
+            gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+
+            QUALIMAP_RNASEQ(bam, gtf)
+            ch_versions = ch_versions.mix(QUALIMAP_RNASEQ.out.versions)
+            ch_qualimap_qc = QUALIMAP_RNASEQ.out.results.ifEmpty(null)
+
+            SAMTOOLS_INDEX_FOR_QC(bam)
+            ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_QC.out.versions)
+
+            bam_indexed = bam.join(SAMTOOLS_INDEX_FOR_QC.out.bai)
+
+            GTF_TO_REFFLAT(gtf)
+            PICARD_COLLECTRNASEQMETRICS(bam_indexed, GTF_TO_REFFLAT.out.refflat, [])
+        }
+    emit:
+        versions        = ch_versions.ifEmpty(null)
+        qualimap_qc     = ch_qualimap_qc.ifEmpty(null)
+        rnaseq_metrics  = PICARD_COLLECTRNASEQMETRICS.out.metrics
+}
+
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index ca1ae5a9..8a71108f 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -18,7 +18,7 @@ def checkPathParamList = [
     params.fasta, params.genomes_base,
     params.ensembl_ref,
     params.fusioncatcher_ref, params.starfusion_ref,
-    params.arriba_ref, params.ericscript_ref,
+    params.arriba_ref, params.starindex_ref,
     params.ensembl_version
 ]
 
@@ -45,10 +45,11 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 //
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
-include { CAT_FASTQ                     }   from '../modules/nf-core/modules/cat/fastq/main'
+
 include { INPUT_CHECK                   }   from '../subworkflows/local/input_check'
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
 include { PIZZLY_WORKFLOW               }   from '../subworkflows/local/pizzly_workflow'
+include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
 include { SQUID_WORKFLOW                }   from '../subworkflows/local/squid_workflow'
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
 include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
@@ -64,6 +65,7 @@ include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionre
 //
 // MODULE: Installed directly from nf-core/modules
 //
+include { CAT_FASTQ                   } from '../modules/nf-core/modules/cat/fastq/main'
 include { FASTQC                      } from '../modules/nf-core/modules/fastqc/main'
 include { MULTIQC                     } from '../modules/nf-core/modules/multiqc/main'
 include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main'
@@ -207,6 +209,13 @@ workflow RNAFUSION {
     )
     ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions.first().ifEmpty(null))
 
+
+    //QC
+    QC_WORKFLOW (
+        STARFUSION_WORKFLOW.out.bam,
+    )
+    ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions.first().ifEmpty(null))
+
 }
 
 

From 9a53161c49b2c1d019866dbec0a4d7dd91f41c8f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 10:57:33 +0100
Subject: [PATCH 212/359] draft gtftogenepred

---
 modules/local/uscs/gtftogenepred/main.nf  | 23 +++++++++++++++
 modules/local/uscs/gtftogenepred/meta.yml | 34 +++++++++++++++++++++++
 2 files changed, 57 insertions(+)
 create mode 100644 modules/local/uscs/gtftogenepred/main.nf
 create mode 100644 modules/local/uscs/gtftogenepred/meta.yml

diff --git a/modules/local/uscs/gtftogenepred/main.nf b/modules/local/uscs/gtftogenepred/main.nf
new file mode 100644
index 00000000..5487e0d1
--- /dev/null
+++ b/modules/local/uscs/gtftogenepred/main.nf
@@ -0,0 +1,23 @@
+process GTF_TO_REFFLAT {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::ucsc-gtftogenepred=377" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ucsc-gtftogenepred:377--ha8a8165_5' :
+        'quay.io/biocontainers/ucsc-gtftogenepred:377--ha8a8165_5' }"
+
+    input:
+    path gtf
+
+    output:
+    path('*.refflat'), emit: refflat
+
+    script:
+    def genepred = ${gtf}.getSimpleName() + '.genepred'
+    def refflat = ${gtf}.getSimpleName() + '.refflat'
+    """
+    gtfToGenePred -genePredExt -geneNameAsName2 ${gtf} ${genepred}
+    paste ${genepred} ${genepred} | cut -f12,16-25 > ${refflat}
+    """
+}
diff --git a/modules/local/uscs/gtftogenepred/meta.yml b/modules/local/uscs/gtftogenepred/meta.yml
new file mode 100644
index 00000000..896e2ef6
--- /dev/null
+++ b/modules/local/uscs/gtftogenepred/meta.yml
@@ -0,0 +1,34 @@
+name: gtf_to_refflat
+description: generate gene annotations in refFlat form
+  - gtftorefflat
+tools:
+  - gtf_to_refflat:
+      description: generate gene annotations in refFlat form
+      homepage: https://pachterlab.github.io/kallisto/
+      documentation: https://pachterlab.github.io/kallisto/manual
+      tool_dev_url: https://github.com/pachterlab/kallisto
+      doi: ""
+      licence: ['BSD-2-Clause']
+
+input:
+  - fasta:
+      type: file
+      description: genome fasta file
+      pattern: "*.{fasta*}"
+  - reference:
+      type: directory
+      description: Path to kallisto index
+      pattern: "*"
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - fusions:
+      type: file
+      description: fusions
+      pattern: "*.txt"
+
+authors:
+  - "@rannick"

From 93262caa6e55c22a1b0dad2367a3b6a9e099af58 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 21 Mar 2022 11:06:38 +0100
Subject: [PATCH 213/359] add draft paste

---
 modules/local/paste/main.nf | 17 +++++++++++++++++
 1 file changed, 17 insertions(+)
 create mode 100644 modules/local/paste/main.nf

diff --git a/modules/local/paste/main.nf b/modules/local/paste/main.nf
new file mode 100644
index 00000000..b1a89cb9
--- /dev/null
+++ b/modules/local/paste/main.nf
@@ -0,0 +1,17 @@
+process PASTE {
+    tag "$meta.id"
+    label 'process_medium'
+
+    input:
+    path input
+
+    output:
+    path output
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    paste ${input} ${input} | cut -f12,16-25 > ${refflat}
+    """
+}

From e8ec52c23c06f80db84196d36642c8e952adecf2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Mar 2022 15:33:40 +0100
Subject: [PATCH 214/359] install picard/markduplicates

---
 .nf-core.yml                                  |  1 +
 bin/check_samplesheet.py                      |  2 +-
 conf/modules.config                           | 28 ++++++++++
 .../modules/picard/markduplicates/main.nf     | 45 ++++++++++++++++
 .../modules/picard/markduplicates/meta.yml    | 52 +++++++++++++++++++
 5 files changed, 127 insertions(+), 1 deletion(-)
 create mode 100644 modules/nf-core/modules/picard/markduplicates/main.nf
 create mode 100644 modules/nf-core/modules/picard/markduplicates/meta.yml

diff --git a/.nf-core.yml b/.nf-core.yml
index b1370fd9..c797df7e 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -2,3 +2,4 @@ lint:
     files_unchanged:
     # Added to ignore linting error: actions/setup-node@v1 until fixed release
     - .github/workflows/linting.yml
+repository_type: pipeline
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 8d381682..8cc14a81 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -137,7 +137,7 @@ def check_samplesheet(file_in, file_out):
         out_dir = os.path.dirname(file_out)
         make_dir(out_dir)
         with open(file_out, "w") as fout:
-            fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n")
+            fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2", "strandedness"]) + "\n")
             for sample in sorted(sample_mapping_dict.keys()):
 
                 ## Check that multiple runs of the same sample are of the same datatype
diff --git a/conf/modules.config b/conf/modules.config
index b8e646f9..51f1e95b 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -81,7 +81,12 @@ process {
         ]
     }
 
+    withName: FUSIONINSPECTOR {
+        ext.when         = { !params.skip_vis }
+    }
+
     withName: FUSIONREPORT {
+        ext.when         = { !params.skip_vis }
         publishDir = [
             path: { "${params.outdir}/fusionreport/${meta.id}" },
             mode: 'copy',
@@ -105,6 +110,14 @@ process {
         ]
     }
 
+    withName: GTF_TO_REFFLAT {
+        publishDir = [
+            path: { "${params.genomes_base}/ensembl" },
+            mode: 'copy',
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
+    }
+
     withName: KALLISTO_INDEX {
         ext.args = '-k 31'
         publishDir = [
@@ -114,6 +127,15 @@ process {
         ]
     }
 
+    withName: PICARD_COLLECTRNASEQMETRICS {
+        ext.when         = { !params.skip_qc && params.starfusion }
+
+    }
+
+    withName: PICARD_MARKDUPLICATES {
+        ext.when         = { !params.skip_qc && params.starfusion }
+    }
+
     withName: PIZZLY {
         ext.args = "-k 31 --align-score 2 --insert-size 400 --cache index.cache.txt"
         publishDir = [
@@ -123,6 +145,10 @@ process {
         ]
     }
 
+    withName: QUALIMAP_RNASEQ {
+        ext.when         = { !params.skip_qc && params.starfusion }
+    }
+
     withName: SAMPLESHEET_CHECK {
         publishDir = [
             path: { "${params.outdir}/pipeline_info" },
@@ -140,6 +166,7 @@ process {
     }
 
     withName: SAMTOOLS_INDEX_FOR_QC {
+        ext.when         = { !params.skip_qc && params.starfusion }
         publishDir = [
             path: { "${params.outdir}/samtools_index_for_qc" },
             mode: 'copy',
@@ -221,6 +248,7 @@ process {
         ext.args = '--twopassMode Basic \
         --outReadsUnmapped None \
         --readFilesCommand zcat \
+        --outSAMtype BAM SortedByCoordinate \
         --outSAMstrandField intronMotif \
         --outSAMunmapped Within \
         --chimSegmentMin 12 \
diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf
new file mode 100644
index 00000000..5196b6ed
--- /dev/null
+++ b/modules/nf-core/modules/picard/markduplicates/main.nf
@@ -0,0 +1,45 @@
+process PICARD_MARKDUPLICATES {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
+        'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(bam)
+
+    output:
+    tuple val(meta), path("*.bam")        , emit: bam
+    tuple val(meta), path("*.bai")        , optional:true, emit: bai
+    tuple val(meta), path("*.metrics.txt"), emit: metrics
+    path  "versions.yml"                  , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def avail_mem = 3
+    if (!task.memory) {
+        log.info '[Picard MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
+    } else {
+        avail_mem = task.memory.giga
+    }
+    """
+    picard \\
+        -Xmx${avail_mem}g \\
+        MarkDuplicates \\
+        $args \\
+        I=$bam \\
+        O=${prefix}.bam \\
+        M=${prefix}.MarkDuplicates.metrics.txt
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        picard: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/picard/markduplicates/meta.yml b/modules/nf-core/modules/picard/markduplicates/meta.yml
new file mode 100644
index 00000000..842817bc
--- /dev/null
+++ b/modules/nf-core/modules/picard/markduplicates/meta.yml
@@ -0,0 +1,52 @@
+name: picard_markduplicates
+description: Locate and tag duplicate reads in a BAM file
+keywords:
+  - markduplicates
+  - pcr
+  - duplicates
+  - bam
+  - sam
+  - cram
+tools:
+  - picard:
+      description: |
+        A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS)
+        data and formats such as SAM/BAM/CRAM and VCF.
+      homepage: https://broadinstitute.github.io/picard/
+      documentation: https://broadinstitute.github.io/picard/
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM file
+      pattern: "*.{bam}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM file with duplicate reads marked/removed
+      pattern: "*.{bam}"
+  - bai:
+      type: file
+      description: An optional BAM index file. If desired, --CREATE_INDEX must be passed as a flag
+      pattern: "*.{bai}"
+  - metrics:
+      type: file
+      description: Duplicate metrics file generated by picard
+      pattern: "*.{metrics.txt}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
+  - "@projectoriented"

From 830cb7a000667667d32cf7d39cf13eb469f36e74 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Mar 2022 15:38:04 +0100
Subject: [PATCH 215/359] complete qc workflow

---
 modules.json                                  |  3 +
 modules/local/fusioninspector/main.nf         |  3 +
 modules/local/fusionreport/detect/main.nf     |  3 +
 .../local/picard/collectrnaseqmetrics/main.nf | 55 +++++++++++++++++++
 .../picard/collectrnaseqmetrics}/meta.yml     |  6 +-
 modules/local/pizzly/detect/main.nf           |  2 +-
 .../main.nf                                   | 13 ++++-
 .../meta.yml                                  |  0
 .../picard/collctrnaseqmetrics/main.nf        | 36 ------------
 nextflow.config                               |  4 ++
 nextflow_schema.json                          | 54 ++++++++++--------
 subworkflows/local/input_check.nf             |  1 +
 subworkflows/local/qc_workflow.nf             | 43 +++++++++------
 subworkflows/local/starfusion_workflow.nf     |  4 +-
 workflows/build_references.nf                 |  3 +
 workflows/rnafusion.nf                        |  2 +-
 16 files changed, 147 insertions(+), 85 deletions(-)
 create mode 100644 modules/local/picard/collectrnaseqmetrics/main.nf
 rename modules/{nf-core/modules/picard/collctrnaseqmetrics => local/picard/collectrnaseqmetrics}/meta.yml (89%)
 rename modules/local/uscs/{gtftogenepred => custom_gtftogenepred}/main.nf (72%)
 rename modules/local/uscs/{gtftogenepred => custom_gtftogenepred}/meta.yml (100%)
 delete mode 100644 modules/nf-core/modules/picard/collctrnaseqmetrics/main.nf

diff --git a/modules.json b/modules.json
index 07b979de..14395867 100644
--- a/modules.json
+++ b/modules.json
@@ -24,6 +24,9 @@
             "picard/collectwgsmetrics": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
+            "picard/markduplicates": {
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+            },
             "qualimap/rnaseq": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index b6a0ac96..cd575734 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -18,6 +18,9 @@ process FUSIONINSPECTOR {
     path "*"                , emit: output
     path "versions.yml"     , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
     def fasta = params.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 625316b9..e1a12056 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -24,6 +24,9 @@ process FUSIONREPORT {
     tuple val(meta), path("*fusionreport_filtered.tsv")  , emit: fusion_list_filtered
     tuple val(meta), path("*.html")                      , emit: report
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def tools = params.arriba         ? "--arriba ${arriba_fusions} " : ''
     tools    += params.pizzly         ? "--pizzly ${pizzly_fusions} " : ''
diff --git a/modules/local/picard/collectrnaseqmetrics/main.nf b/modules/local/picard/collectrnaseqmetrics/main.nf
new file mode 100644
index 00000000..221f507b
--- /dev/null
+++ b/modules/local/picard/collectrnaseqmetrics/main.nf
@@ -0,0 +1,55 @@
+process PICARD_COLLECTRNASEQMETRICS {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
+        'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+    path(refflat)
+    path(rrna_intervals)
+
+    output:
+    tuple val(meta), path("*rna_metrics.txt")    , emit: metrics
+    path  "versions.yml"                        , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def strandedness = ''
+    // def strandedness = '--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND'
+    if ("${meta.strandedness}" == 'forward') {
+        strandedness = '--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND'
+    } else if ("${meta.strandedness}" == 'reverse') {
+        strandedness = '--STRAND_SPECIFICITY SECOND_READ_TRANSCRIPTION_STRAND'
+    }
+    def rrna = rrna_intervals == [] ? '' : "--RIBOSOMAL_INTERVALS ${rrna_intervals}"
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def avail_mem = 3
+    if (!task.memory) {
+        log.info '[Picard CollectRnaMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
+    } else {
+        avail_mem = task.memory.giga
+    }
+    """
+    picard \\
+        -Xmx${avail_mem}g \\
+        CollectRnaSeqMetrics \\
+        --TMP_DIR ./tmp \\
+        ${strandedness} \\
+        ${rrna} \\
+        --REF_FLAT ${refflat} \\
+        --INPUT ${bam} \\
+        --OUTPUT ${prefix}_rna_metrics.txt \\
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        picard: \$(picard CollectRnaMetrics --version 2>&1 | grep -o 'Version.*' | cut -f2- -d:)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/picard/collctrnaseqmetrics/meta.yml b/modules/local/picard/collectrnaseqmetrics/meta.yml
similarity index 89%
rename from modules/nf-core/modules/picard/collctrnaseqmetrics/meta.yml
rename to modules/local/picard/collectrnaseqmetrics/meta.yml
index 75541034..131b8337 100644
--- a/modules/nf-core/modules/picard/collctrnaseqmetrics/meta.yml
+++ b/modules/local/picard/collectrnaseqmetrics/meta.yml
@@ -1,5 +1,5 @@
 name: picard_collectrnaseqmetrics
-description: Produces RNA alignment metrics for a SAM or BAM file. 
+description: Produces RNA alignment metrics for a SAM or BAM file.
 keywords:
   - alignment
   - metrics
@@ -25,6 +25,10 @@ input:
       type: file
       description: BAM file
       pattern: "*.{bam}"
+  - bai:
+      type: file
+      description: An optional BAM index file. If desired, --CREATE_INDEX must be passed as a flag
+      pattern: "*.{bai}"
   - refflat:
       type: file
       description: Gene annotations in refFlat form
diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index 8fef1475..376dc7f2 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -6,7 +6,7 @@ process PIZZLY {
     if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
         container "https://depot.galaxyproject.org/singularity/pizzly:0.37.3--py36_2"
     } else {
-        container "quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1"
+        container "quay.io/biocontainers/pizzly:0.37.3--h470a237_3"
     }
 
     input:
diff --git a/modules/local/uscs/gtftogenepred/main.nf b/modules/local/uscs/custom_gtftogenepred/main.nf
similarity index 72%
rename from modules/local/uscs/gtftogenepred/main.nf
rename to modules/local/uscs/custom_gtftogenepred/main.nf
index 5487e0d1..1b2a22bc 100644
--- a/modules/local/uscs/gtftogenepred/main.nf
+++ b/modules/local/uscs/custom_gtftogenepred/main.nf
@@ -1,5 +1,4 @@
 process GTF_TO_REFFLAT {
-    tag "$meta.id"
     label 'process_medium'
 
     conda (params.enable_conda ? "bioconda::ucsc-gtftogenepred=377" : null)
@@ -7,17 +6,25 @@ process GTF_TO_REFFLAT {
         'https://depot.galaxyproject.org/singularity/ucsc-gtftogenepred:377--ha8a8165_5' :
         'quay.io/biocontainers/ucsc-gtftogenepred:377--ha8a8165_5' }"
 
+//TODO easier with meta/or add to ensembl
     input:
     path gtf
 
     output:
     path('*.refflat'), emit: refflat
 
+
+    // TODO:add version
     script:
-    def genepred = ${gtf}.getSimpleName() + '.genepred'
-    def refflat = ${gtf}.getSimpleName() + '.refflat'
+    def genepred = gtf + '.genepred'
+    def refflat = gtf + '.refflat'
     """
     gtfToGenePred -genePredExt -geneNameAsName2 ${gtf} ${genepred}
     paste ${genepred} ${genepred} | cut -f12,16-25 > ${refflat}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gtfToGenePred: 377
+    END_VERSIONS
     """
 }
diff --git a/modules/local/uscs/gtftogenepred/meta.yml b/modules/local/uscs/custom_gtftogenepred/meta.yml
similarity index 100%
rename from modules/local/uscs/gtftogenepred/meta.yml
rename to modules/local/uscs/custom_gtftogenepred/meta.yml
diff --git a/modules/nf-core/modules/picard/collctrnaseqmetrics/main.nf b/modules/nf-core/modules/picard/collctrnaseqmetrics/main.nf
deleted file mode 100644
index dbbc7198..00000000
--- a/modules/nf-core/modules/picard/collctrnaseqmetrics/main.nf
+++ /dev/null
@@ -1,36 +0,0 @@
-process PICARD_COLLECTRNASEQMETRICS{
-
-    input:
-        tuple val(sample), path(bam), path(bai)
-        path(refflat)
-        path(rrna_intervals)
-
-    output:
-        tuple val(sample), path("${sample}_rna_metrics.txt"), emit metrics
-
-    script:
-    def strandedness = ''
-    if (params.strandedness == 'forward') {
-        strandedness = '--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND'
-    } else if (params.strandedness == 'reverse') {
-        strandedness = '--STRAND_SPECIFICITY SECOND_READ_TRANSCRIPTION_STRAND'
-    }
-    def rrna = rrna_intervals == [] ? '' : "--RIBOSOMAL_INTERVALS ${rrna_intervals}"
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def avail_mem = 3
-    if (!task.memory) {
-        log.info '[Picard CollectWgsMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = task.memory.giga
-    }
-    """
-    picard CollectRnaSeqMetrics \\
-        --TMP_DIR ./tmp \\
-        ${strandedness} \\
-        ${rrna} \\
-        --REF_FLAT ${refflat} \\
-        --INPUT ${bam} \\
-        --OUTPUT ${prefix}_rna_metrics.txt \\
-    """
-}
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index 9d1c283d..e5bd1867 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -45,6 +45,10 @@ params {
     starfusion                 = false
     fusionreport               = false
 
+    // Skip steps
+    skip_qc                    = false
+    skip_vis                   = false
+
     // Path to references
     arriba_ref                    = "${params.genomes_base}/arriba"
     ensembl_ref                   = "${params.genomes_base}/ensembl"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 020ded1c..05b64ae2 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -5,14 +5,28 @@
     "description": "Nextflow rnafusion analysis pipeline, part of the nf-core community.",
     "type": "object",
     "definitions": {
+        "skip_steps": {
+            "title": "Skip steps",
+            "type": "object",
+            "description": "Skip analysis steps",
+            "default": "",
+            "properties": {
+                "skip_qc": {
+                    "type": "boolean",
+                    "description": "Skip QC steps"
+                },
+                "skip_vis": {
+                    "type": "boolean",
+                    "description": "Skip visualisation steps"
+                }
+            },
+            "fa_icon": "fas fa-fast-forward"
+        },
         "input_output_options": {
             "title": "Input/output options",
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": [
-                "input"
-            ],
             "properties": {
                 "input": {
                     "type": "string",
@@ -61,19 +75,17 @@
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
                     "description": "Read length",
-                    "default": "100"
+                    "default": 100
                 },
                 "all": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run all references/analyses",
-                    "default": "false"
+                    "description": "Build or run all references/analyses"
                 },
                 "arriba": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run arriba references/analyses",
-                    "default": "false"
+                    "description": "Build or run arriba references/analyses"
                 },
                 "arriba_ref": {
                     "type": "string",
@@ -93,8 +105,7 @@
                 "ericscript": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run ericscript references/analyses",
-                    "default": "false"
+                    "description": "Build or run ericscript references/analyses"
                 },
                 "ericscript_ref": {
                     "type": "string",
@@ -104,8 +115,7 @@
                 "fusioncatcher": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run fusioncatcher references/analyses",
-                    "default": "false"
+                    "description": "Build or run fusioncatcher references/analyses"
                 },
                 "fusioncatcher_fusions": {
                     "type": "string",
@@ -120,8 +130,7 @@
                 "fusionreport": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build fusionreport references",
-                    "default": "false"
+                    "description": "Build fusionreport references"
                 },
                 "fusionreport_ref": {
                     "type": "string",
@@ -131,8 +140,7 @@
                 "pizzly": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run pizzly references/analyses",
-                    "default": "false"
+                    "description": "Build or run pizzly references/analyses"
                 },
                 "pizzly_fusions": {
                     "type": "string",
@@ -147,8 +155,7 @@
                 "squid": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run squid references/analyses",
-                    "default": "false"
+                    "description": "Build or run squid references/analyses"
                 },
                 "squid_fusions": {
                     "type": "string",
@@ -163,8 +170,7 @@
                 "starfusion": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run starfusion references/analyses",
-                    "default": "false"
+                    "description": "Build or run starfusion references/analyses"
                 },
                 "starfusion_fusions": {
                     "type": "string",
@@ -179,8 +185,7 @@
                 "starindex": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run starindex references/analyses",
-                    "default": "false"
+                    "description": "Build or run starindex references/analyses"
                 },
                 "starindex_ref": {
                     "type": "string",
@@ -411,6 +416,9 @@
         }
     },
     "allOf": [
+        {
+            "$ref": "#/definitions/skip_steps"
+        },
         {
             "$ref": "#/definitions/input_output_options"
         },
@@ -427,4 +435,4 @@
             "$ref": "#/definitions/generic_options"
         }
     ]
-}
+}
\ No newline at end of file
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index cddcbb3c..6e8a6a70 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -25,6 +25,7 @@ def create_fastq_channels(LinkedHashMap row) {
     def meta = [:]
     meta.id           = row.sample
     meta.single_end   = row.single_end.toBoolean()
+    meta.strandedness = row.strandedness
 
     def array = []
     if (!file(row.fastq_1).exists()) {
diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index a453d136..c97a1093 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -2,36 +2,43 @@
 // Check input samplesheet and get read channels
 //
 
-include { QUALIMAP_RNASEQ }    from '../../modules/nf-core/modules/qualimap/rnaseq/main'
-include { GTF_TO_REFFLAT }     from '../../modules/local/uscs/gtftogenepred/main'
-
+include { QUALIMAP_RNASEQ }                            from '../../modules/nf-core/modules/qualimap/rnaseq/main'
+include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_QC }    from '../../modules/nf-core/modules/samtools/index/main'
+include { PICARD_COLLECTRNASEQMETRICS }                 from '../../modules/local/picard/collectrnaseqmetrics/main'
+include { PICARD_MARKDUPLICATES }                      from '../../modules/nf-core/modules/picard/markduplicates/main'
 
 workflow QC_WORKFLOW {
     take:
-        bam
+        bam_sorted
 
     main:
         ch_versions = Channel.empty()
         ch_qualimap_qc = Channel.empty()
 
-        if (params.starfusion && params.qc){
-            gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
+        refflat ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf.refflat"
+
+        QUALIMAP_RNASEQ(bam_sorted, gtf)
+        ch_versions = ch_versions.mix(QUALIMAP_RNASEQ.out.versions)
+        ch_qualimap_qc = QUALIMAP_RNASEQ.out.results.ifEmpty(null)
 
-            QUALIMAP_RNASEQ(bam, gtf)
-            ch_versions = ch_versions.mix(QUALIMAP_RNASEQ.out.versions)
-            ch_qualimap_qc = QUALIMAP_RNASEQ.out.results.ifEmpty(null)
+        SAMTOOLS_INDEX_FOR_QC(bam_sorted)
+        ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_QC.out.versions)
 
-            SAMTOOLS_INDEX_FOR_QC(bam)
-            ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_QC.out.versions)
+        bam_indexed = bam_sorted.join(SAMTOOLS_INDEX_FOR_QC.out.bai)
 
-            bam_indexed = bam.join(SAMTOOLS_INDEX_FOR_QC.out.bai)
+        PICARD_COLLECTRNASEQMETRICS(bam_indexed, refflat, [])
+        ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
 
-            GTF_TO_REFFLAT(gtf)
-            PICARD_COLLECTRNASEQMETRICS(bam_indexed, GTF_TO_REFFLAT.out.refflat, [])
-        }
+        PICARD_MARKDUPLICATES(bam_sorted)
+        ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES.out.versions)
+
+// TODO: check what happens with skip qc
     emit:
-        versions        = ch_versions.ifEmpty(null)
-        qualimap_qc     = ch_qualimap_qc.ifEmpty(null)
-        rnaseq_metrics  = PICARD_COLLECTRNASEQMETRICS.out.metrics
+        versions            = ch_versions.ifEmpty(null)
+        qualimap_qc         = ch_qualimap_qc.ifEmpty(null)
+        rnaseq_metrics      = PICARD_COLLECTRNASEQMETRICS.out.metrics
+        duplicate_metrics   = PICARD_MARKDUPLICATES.out.metrics
+
 }
 
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index 837a7979..f25227cc 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -29,7 +29,7 @@ workflow STARFUSION_WORKFLOW {
 
                 STAR_FOR_STARFUSION( reads, params.starindex_ref, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
-                ch_align = STAR_FOR_STARFUSION.out.bam
+                ch_align = STAR_FOR_STARFUSION.out.bam_sorted
 
                 reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
 
@@ -45,7 +45,7 @@ workflow STARFUSION_WORKFLOW {
         }
     emit:
         fusions         = ch_starfusion_fusions
-        bam             = ch_align.ifEmpty(null)
+        bam_sorted      = ch_align
         versions        = ch_versions.ifEmpty(null)
 
 
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index ad9c3f33..a3061216 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -10,6 +10,7 @@ include { ERICSCRIPT_DOWNLOAD }             from '../modules/local/ericscript/do
 include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/download/main'
 include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
+include { GTF_TO_REFFLAT }                  from '../modules/local/uscs/custom_gtftogenepred/main'
 
 /*
 ========================================================================================
@@ -30,6 +31,8 @@ workflow BUILD_REFERENCES {
 
     ENSEMBL_DOWNLOAD( params.ensembl_version )
 
+    GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
+
     if (params.starindex || params.all) {
         STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
     }
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 8a71108f..bc5a5971 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -212,7 +212,7 @@ workflow RNAFUSION {
 
     //QC
     QC_WORKFLOW (
-        STARFUSION_WORKFLOW.out.bam,
+        STARFUSION_WORKFLOW.out.bam_sorted
     )
     ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions.first().ifEmpty(null))
 

From 841192b8bc1b7d5d13721169f76826bc3d525f87 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Mar 2022 15:52:24 +0100
Subject: [PATCH 216/359] linting

---
 .nf-core.yml | 1 -
 1 file changed, 1 deletion(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index 51516928..7036b988 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -5,4 +5,3 @@ lint:
     - .github/workflows/linting.yml
     - .github/workflows/branch.yml
     - .github/workflows/linting_comment.yml
-    
\ No newline at end of file

From 74f545b3cf935432ad64c7d12a44a54b7ca05629 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Mar 2022 16:58:21 +0100
Subject: [PATCH 217/359] fix check samplesheet mergin error

---
 bin/check_samplesheet.py | 13 +++++++------
 1 file changed, 7 insertions(+), 6 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 58fa4455..f7f703a3 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -153,17 +153,18 @@ def sniff_format(handle):
     handle.seek(0)
     return dialect
 
+def check_samplesheet(file_in, file_out):
+    """
+    This function checks that the samplesheet follows the following structure:
     sample,fastq_1,fastq_2,strandedness
     SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,forward
     SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,forward
-
-
-def check_samplesheet(file_in, file_out):
+    For an example see:
+    https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
     """
-    Check that the tabular samplesheet has the structure expected by nf-core pipelines.
 
-    Validate the general shape of the table, expected columns, and each row. Also add
-    an additional column which records whether one or two FASTQ reads were found.
+    sample_mapping_dict = {}
+    with open(file_in, "r") as fin:
 
         ## Check header
         MIN_COLS = 2

From 23e5ee695e344bd90cae01cbd2957250ee6d9cf2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Mar 2022 17:04:38 +0100
Subject: [PATCH 218/359] fix check samplesheet mergin error2

---
 bin/check_samplesheet.py | 12 ++++++++++++
 1 file changed, 12 insertions(+)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index f7f703a3..74e767fe 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -8,6 +8,7 @@
 import csv
 import logging
 import sys
+import os
 from collections import Counter
 from pathlib import Path
 
@@ -61,6 +62,17 @@ def __init__(
         self._seen = set()
         self.modified = []
 
+def parse_args(args=None):
+    Description = "Reformat nf-core/rnafusion samplesheet file and check its contents."
+    Epilog = "Example usage: python check_samplesheet.py <FILE_IN> <FILE_OUT>"
+
+    parser = argparse.ArgumentParser(description=Description, epilog=Epilog)
+    parser.add_argument("FILE_IN", help="Input samplesheet file.")
+    parser.add_argument("FILE_OUT", help="Output file.")
+    return parser.parse_args(args)
+
+
+
     def validate_and_transform(self, row):
         """
         Perform all validations on the given row and insert the read pairing status.

From bc9d6d502d64d63275d448efcb896f984349bf01 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Mar 2022 17:11:29 +0100
Subject: [PATCH 219/359] fix check samplesheet mergin error3

---
 bin/check_samplesheet.py | 167 +++++----------------------------------
 1 file changed, 21 insertions(+), 146 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 74e767fe..8cc14a81 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -1,66 +1,13 @@
 #!/usr/bin/env python
 
+# TODO nf-core: Update the script to check the samplesheet
+# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
 
-"""Provide a command line tool to validate and transform tabular samplesheets."""
-
-
-import argparse
-import csv
-import logging
-import sys
 import os
-from collections import Counter
-from pathlib import Path
-
-
-logger = logging.getLogger()
-
-
-class RowChecker:
-    """
-    Define a service that can validate and transform each given row.
-
-    Attributes:
-        modified (list): A list of dicts, where each dict corresponds to a previously
-            validated and transformed row. The order of rows is maintained.
-
-    """
-
-    VALID_FORMATS = (
-        ".fq.gz",
-        ".fastq.gz",
-    )
-
-    def __init__(
-        self,
-        sample_col="sample",
-        first_col="fastq_1",
-        second_col="fastq_2",
-        single_col="single_end",
-        **kwargs,
-    ):
-        """
-        Initialize the row checker with the expected column names.
-
-        Args:
-            sample_col (str): The name of the column that contains the sample name
-                (default "sample").
-            first_col (str): The name of the column that contains the first (or only)
-                FASTQ file path (default "fastq_1").
-            second_col (str): The name of the column that contains the second (if any)
-                FASTQ file path (default "fastq_2").
-            single_col (str): The name of the new column that will be inserted and
-                records whether the sample contains single- or paired-end sequencing
-                reads (default "single_end").
+import sys
+import errno
+import argparse
 
-        """
-        super().__init__(**kwargs)
-        self._sample_col = sample_col
-        self._first_col = first_col
-        self._second_col = second_col
-        self._single_col = single_col
-        self._seen = set()
-        self.modified = []
 
 def parse_args(args=None):
     Description = "Reformat nf-core/rnafusion samplesheet file and check its contents."
@@ -72,105 +19,33 @@ def parse_args(args=None):
     return parser.parse_args(args)
 
 
+def make_dir(path):
+    if len(path) > 0:
+        try:
+            os.makedirs(path)
+        except OSError as exception:
+            if exception.errno != errno.EEXIST:
+                raise exception
 
-    def validate_and_transform(self, row):
-        """
-        Perform all validations on the given row and insert the read pairing status.
-
-        Args:
-            row (dict): A mapping from column headers (keys) to elements of that row
-                (values).
 
-        """
-        self._validate_sample(row)
-        self._validate_first(row)
-        self._validate_second(row)
-        self._validate_pair(row)
-        self._seen.add((row[self._sample_col], row[self._first_col]))
-        self.modified.append(row)
-
-    def _validate_sample(self, row):
-        """Assert that the sample name exists and convert spaces to underscores."""
-        assert len(row[self._sample_col]) > 0, "Sample input is required."
-        # Sanitize samples slightly.
-        row[self._sample_col] = row[self._sample_col].replace(" ", "_")
-
-    def _validate_first(self, row):
-        """Assert that the first FASTQ entry is non-empty and has the right format."""
-        assert len(row[self._first_col]) > 0, "At least the first FASTQ file is required."
-        self._validate_fastq_format(row[self._first_col])
-
-    def _validate_second(self, row):
-        """Assert that the second FASTQ entry has the right format if it exists."""
-        if len(row[self._second_col]) > 0:
-            self._validate_fastq_format(row[self._second_col])
-
-    def _validate_pair(self, row):
-        """Assert that read pairs have the same file extension. Report pair status."""
-        if row[self._first_col] and row[self._second_col]:
-            row[self._single_col] = False
-            assert (
-                Path(row[self._first_col]).suffixes == Path(row[self._second_col]).suffixes
-            ), "FASTQ pairs must have the same file extensions."
-        else:
-            row[self._single_col] = True
-
-    def _validate_fastq_format(self, filename):
-        """Assert that a given filename has one of the expected FASTQ extensions."""
-        assert any(filename.endswith(extension) for extension in self.VALID_FORMATS), (
-            f"The FASTQ file has an unrecognized extension: {filename}\n"
-            f"It should be one of: {', '.join(self.VALID_FORMATS)}"
+def print_error(error, context="Line", context_str=""):
+    error_str = "ERROR: Please check samplesheet -> {}".format(error)
+    if context != "" and context_str != "":
+        error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format(
+            error, context.strip(), context_str.strip()
         )
+    print(error_str)
+    sys.exit(1)
 
-    def validate_unique_samples(self):
-        """
-        Assert that the combination of sample name and FASTQ filename is unique.
-
-        In addition to the validation, also rename the sample if more than one sample,
-        FASTQ file combination exists.
-
-        """
-        assert len(self._seen) == len(self.modified), "The pair of sample name and FASTQ must be unique."
-        if len({pair[0] for pair in self._seen}) < len(self._seen):
-            counts = Counter(pair[0] for pair in self._seen)
-            seen = Counter()
-            for row in self.modified:
-                sample = row[self._sample_col]
-                seen[sample] += 1
-                if counts[sample] > 1:
-                    row[self._sample_col] = f"{sample}_T{seen[sample]}"
-
-
-def sniff_format(handle):
-    """
-    Detect the tabular format.
-
-    Args:
-        handle (text file): A handle to a `text file`_ object. The read position is
-        expected to be at the beginning (index 0).
-
-    Returns:
-        csv.Dialect: The detected tabular format.
-
-    .. _text file:
-        https://docs.python.org/3/glossary.html#term-text-file
-
-    """
-    peek = handle.read(2048)
-    sniffer = csv.Sniffer()
-    if not sniffer.has_header(peek):
-        logger.critical(f"The given sample sheet does not appear to contain a header.")
-        sys.exit(1)
-    dialect = sniffer.sniff(peek)
-    handle.seek(0)
-    return dialect
 
 def check_samplesheet(file_in, file_out):
     """
     This function checks that the samplesheet follows the following structure:
+
     sample,fastq_1,fastq_2,strandedness
     SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,forward
     SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,forward
+
     For an example see:
     https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
     """

From 5398fb14425641f7ac53e512ad798da54480175a Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <apeltzer@users.noreply.github.com>
Date: Tue, 22 Mar 2022 20:05:42 +0100
Subject: [PATCH 220/359] Update check_samplesheet.py

---
 bin/check_samplesheet.py | 169 +++++++--------------------------------
 1 file changed, 28 insertions(+), 141 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index f7f703a3..8cc14a81 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -1,164 +1,51 @@
 #!/usr/bin/env python
 
+# TODO nf-core: Update the script to check the samplesheet
+# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
 
-"""Provide a command line tool to validate and transform tabular samplesheets."""
-
-
-import argparse
-import csv
-import logging
+import os
 import sys
-from collections import Counter
-from pathlib import Path
-
-
-logger = logging.getLogger()
-
-
-class RowChecker:
-    """
-    Define a service that can validate and transform each given row.
-
-    Attributes:
-        modified (list): A list of dicts, where each dict corresponds to a previously
-            validated and transformed row. The order of rows is maintained.
-
-    """
-
-    VALID_FORMATS = (
-        ".fq.gz",
-        ".fastq.gz",
-    )
-
-    def __init__(
-        self,
-        sample_col="sample",
-        first_col="fastq_1",
-        second_col="fastq_2",
-        single_col="single_end",
-        **kwargs,
-    ):
-        """
-        Initialize the row checker with the expected column names.
-
-        Args:
-            sample_col (str): The name of the column that contains the sample name
-                (default "sample").
-            first_col (str): The name of the column that contains the first (or only)
-                FASTQ file path (default "fastq_1").
-            second_col (str): The name of the column that contains the second (if any)
-                FASTQ file path (default "fastq_2").
-            single_col (str): The name of the new column that will be inserted and
-                records whether the sample contains single- or paired-end sequencing
-                reads (default "single_end").
-
-        """
-        super().__init__(**kwargs)
-        self._sample_col = sample_col
-        self._first_col = first_col
-        self._second_col = second_col
-        self._single_col = single_col
-        self._seen = set()
-        self.modified = []
-
-    def validate_and_transform(self, row):
-        """
-        Perform all validations on the given row and insert the read pairing status.
+import errno
+import argparse
 
-        Args:
-            row (dict): A mapping from column headers (keys) to elements of that row
-                (values).
 
-        """
-        self._validate_sample(row)
-        self._validate_first(row)
-        self._validate_second(row)
-        self._validate_pair(row)
-        self._seen.add((row[self._sample_col], row[self._first_col]))
-        self.modified.append(row)
+def parse_args(args=None):
+    Description = "Reformat nf-core/rnafusion samplesheet file and check its contents."
+    Epilog = "Example usage: python check_samplesheet.py <FILE_IN> <FILE_OUT>"
 
-    def _validate_sample(self, row):
-        """Assert that the sample name exists and convert spaces to underscores."""
-        assert len(row[self._sample_col]) > 0, "Sample input is required."
-        # Sanitize samples slightly.
-        row[self._sample_col] = row[self._sample_col].replace(" ", "_")
+    parser = argparse.ArgumentParser(description=Description, epilog=Epilog)
+    parser.add_argument("FILE_IN", help="Input samplesheet file.")
+    parser.add_argument("FILE_OUT", help="Output file.")
+    return parser.parse_args(args)
 
-    def _validate_first(self, row):
-        """Assert that the first FASTQ entry is non-empty and has the right format."""
-        assert len(row[self._first_col]) > 0, "At least the first FASTQ file is required."
-        self._validate_fastq_format(row[self._first_col])
 
-    def _validate_second(self, row):
-        """Assert that the second FASTQ entry has the right format if it exists."""
-        if len(row[self._second_col]) > 0:
-            self._validate_fastq_format(row[self._second_col])
+def make_dir(path):
+    if len(path) > 0:
+        try:
+            os.makedirs(path)
+        except OSError as exception:
+            if exception.errno != errno.EEXIST:
+                raise exception
 
-    def _validate_pair(self, row):
-        """Assert that read pairs have the same file extension. Report pair status."""
-        if row[self._first_col] and row[self._second_col]:
-            row[self._single_col] = False
-            assert (
-                Path(row[self._first_col]).suffixes == Path(row[self._second_col]).suffixes
-            ), "FASTQ pairs must have the same file extensions."
-        else:
-            row[self._single_col] = True
 
-    def _validate_fastq_format(self, filename):
-        """Assert that a given filename has one of the expected FASTQ extensions."""
-        assert any(filename.endswith(extension) for extension in self.VALID_FORMATS), (
-            f"The FASTQ file has an unrecognized extension: {filename}\n"
-            f"It should be one of: {', '.join(self.VALID_FORMATS)}"
+def print_error(error, context="Line", context_str=""):
+    error_str = "ERROR: Please check samplesheet -> {}".format(error)
+    if context != "" and context_str != "":
+        error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format(
+            error, context.strip(), context_str.strip()
         )
+    print(error_str)
+    sys.exit(1)
 
-    def validate_unique_samples(self):
-        """
-        Assert that the combination of sample name and FASTQ filename is unique.
-
-        In addition to the validation, also rename the sample if more than one sample,
-        FASTQ file combination exists.
-
-        """
-        assert len(self._seen) == len(self.modified), "The pair of sample name and FASTQ must be unique."
-        if len({pair[0] for pair in self._seen}) < len(self._seen):
-            counts = Counter(pair[0] for pair in self._seen)
-            seen = Counter()
-            for row in self.modified:
-                sample = row[self._sample_col]
-                seen[sample] += 1
-                if counts[sample] > 1:
-                    row[self._sample_col] = f"{sample}_T{seen[sample]}"
-
-
-def sniff_format(handle):
-    """
-    Detect the tabular format.
-
-    Args:
-        handle (text file): A handle to a `text file`_ object. The read position is
-        expected to be at the beginning (index 0).
-
-    Returns:
-        csv.Dialect: The detected tabular format.
-
-    .. _text file:
-        https://docs.python.org/3/glossary.html#term-text-file
-
-    """
-    peek = handle.read(2048)
-    sniffer = csv.Sniffer()
-    if not sniffer.has_header(peek):
-        logger.critical(f"The given sample sheet does not appear to contain a header.")
-        sys.exit(1)
-    dialect = sniffer.sniff(peek)
-    handle.seek(0)
-    return dialect
 
 def check_samplesheet(file_in, file_out):
     """
     This function checks that the samplesheet follows the following structure:
+
     sample,fastq_1,fastq_2,strandedness
     SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,forward
     SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,forward
+
     For an example see:
     https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
     """

From bdb2e1aac8532e1b0c94a75fd44bec7f1996a417 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Tue, 22 Mar 2022 20:10:47 +0100
Subject: [PATCH 221/359] Revert samplesheet to working version

---
 bin/check_samplesheet.py | 53 ++++++++++------------------------------
 1 file changed, 13 insertions(+), 40 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 8cc14a81..ddc6016a 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -1,6 +1,4 @@
-#!/usr/bin/env python
-
-# TODO nf-core: Update the script to check the samplesheet
+ nf-core: Update the script to check the samplesheet
 # This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
 
 import os
@@ -42,9 +40,10 @@ def check_samplesheet(file_in, file_out):
     """
     This function checks that the samplesheet follows the following structure:
 
-    sample,fastq_1,fastq_2,strandedness
-    SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,forward
-    SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,forward
+    sample,fastq_1,fastq_2
+    SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz
+    SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz
+    SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,
 
     For an example see:
     https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
@@ -55,7 +54,7 @@ def check_samplesheet(file_in, file_out):
 
         ## Check header
         MIN_COLS = 2
-        HEADER = ["sample", "fastq_1", "fastq_2", "strandedness"]
+        HEADER = ["sample", "fastq_1", "fastq_2"]
         header = [x.strip('"') for x in fin.readline().strip().split(",")]
         if header[: len(HEADER)] != HEADER:
             print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
@@ -81,7 +80,7 @@ def check_samplesheet(file_in, file_out):
                 )
 
             ## Check sample name entries
-            sample, fastq_1, fastq_2 , strandedness = lspl[: len(HEADER)]
+            sample, fastq_1, fastq_2 = lspl[: len(HEADER)]
             sample = sample.replace(" ", "_")
             if not sample:
                 print_error("Sample entry has not been specified!", "Line", line)
@@ -98,32 +97,16 @@ def check_samplesheet(file_in, file_out):
                             line,
                         )
 
-            ## Check strandedness
-            strandednesses = ["unstranded", "forward", "reverse"]
-            if strandedness:
-                if strandedness not in strandednesses:
-                    print_error(
-                        f"Strandedness must be one of '{', '.join(strandednesses)}'!",
-                        "Line",
-                        line,
-                    )
-            else:
-                print_error(
-                    f"Strandedness has not been specified! Must be one of {', '.join(strandednesses)}.",
-                    "Line",
-                    line,
-                )
-
             ## Auto-detect paired-end/single-end
-            sample_info = []  ## [single_end, fastq_1, fastq_2, strandedness]
+            sample_info = []  ## [single_end, fastq_1, fastq_2]
             if sample and fastq_1 and fastq_2:  ## Paired-end short reads
-                sample_info = ["0", fastq_1, fastq_2, strandedness]
+                sample_info = ["0", fastq_1, fastq_2]
             elif sample and fastq_1 and not fastq_2:  ## Single-end short reads
-                sample_info = ["1", fastq_1, fastq_2, strandedness]
+                sample_info = ["1", fastq_1, fastq_2]
             else:
                 print_error("Invalid combination of columns provided!", "Line", line)
 
-            ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2, strandedness ] }
+            ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] }
             if sample not in sample_mapping_dict:
                 sample_mapping_dict[sample] = [sample_info]
             else:
@@ -137,24 +120,13 @@ def check_samplesheet(file_in, file_out):
         out_dir = os.path.dirname(file_out)
         make_dir(out_dir)
         with open(file_out, "w") as fout:
-            fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2", "strandedness"]) + "\n")
+            fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n")
             for sample in sorted(sample_mapping_dict.keys()):
 
                 ## Check that multiple runs of the same sample are of the same datatype
                 if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]):
                     print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample))
 
-                ## Check that multiple runs of the same sample are of the same strandedness
-                if not all(
-                    x[-1] == sample_mapping_dict[sample][0][-1]
-                    for x in sample_mapping_dict[sample]
-                ):
-                    print_error(
-                        f"Multiple runs of a sample must have the same strandedness!",
-                        "Sample",
-                        sample,
-                    )
-
                 for idx, val in enumerate(sample_mapping_dict[sample]):
                     fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n")
     else:
@@ -168,3 +140,4 @@ def main(args=None):
 
 if __name__ == "__main__":
     sys.exit(main())
+

From 32ad99f139a8657ab12a7efc3a34c4dca2985bb6 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Wed, 23 Mar 2022 13:53:40 +0000
Subject: [PATCH 222/359] Template update for nf-core/tools version 2.3.1

---
 .editorconfig                                 |   5 +-
 .github/CONTRIBUTING.md                       |  15 +-
 .github/ISSUE_TEMPLATE/bug_report.yml         |   1 -
 .github/PULL_REQUEST_TEMPLATE.md              |   6 +-
 .github/workflows/awsfulltest.yml             |   1 -
 .github/workflows/awstest.yml                 |   2 +-
 .github/workflows/branch.yml                  |   5 +-
 .github/workflows/ci.yml                      |  12 +-
 .github/workflows/linting.yml                 |  85 ++---------
 .github/workflows/linting_comment.yml         |   3 +-
 .gitpod.yml                                   |  16 +-
 .markdownlint.yml                             |  14 --
 .prettierrc.yml                               |   1 +
 .yamllint.yml                                 |   6 -
 CHANGELOG.md                                  |   2 +
 CITATIONS.md                                  |  27 ++--
 README.md                                     |  31 ++--
 assets/email_template.html                    | 142 ++++++++++++------
 assets/multiqc_config.yaml                    |  11 --
 assets/multiqc_config.yml                     |  11 ++
 assets/schema_input.json                      |   5 +-
 docs/README.md                                |   8 +-
 docs/output.md                                |  28 ++--
 docs/usage.md                                 | 109 +++++++-------
 modules.json                                  |   8 +-
 .../custom/dumpsoftwareversions/main.nf       |   3 +
 .../custom/dumpsoftwareversions/meta.yml      |   2 +-
 modules/nf-core/modules/fastqc/main.nf        |   3 +
 modules/nf-core/modules/fastqc/meta.yml       |  90 +++++------
 modules/nf-core/modules/multiqc/main.nf       |   9 +-
 modules/nf-core/modules/multiqc/meta.yml      |  66 ++++----
 nextflow_schema.json                          |  14 +-
 workflows/rnafusion.nf                        |   2 +-
 33 files changed, 361 insertions(+), 382 deletions(-)
 delete mode 100644 .markdownlint.yml
 create mode 100644 .prettierrc.yml
 delete mode 100644 .yamllint.yml
 delete mode 100644 assets/multiqc_config.yaml
 create mode 100644 assets/multiqc_config.yml

diff --git a/.editorconfig b/.editorconfig
index 95549501..b6b31907 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -8,12 +8,9 @@ trim_trailing_whitespace = true
 indent_size = 4
 indent_style = space
 
-[*.{yml,yaml}]
+[*.{md,yml,yaml,html,css,scss,js}]
 indent_size = 2
 
-[*.json]
-insert_final_newline = unset
-
 # These files are edited and tested upstream in nf-core/modules
 [/modules/nf-core/**]
 charset = unset
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 4dfbe44c..9ae9e986 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -15,8 +15,7 @@ Contributions to the code are even more welcome ;)
 
 If you'd like to write some code for nf-core/rnafusion, the standard workflow is as follows:
 
-1. Check that there isn't already an issue about your idea in the [nf-core/rnafusion issues](https://github.com/nf-core/rnafusion/issues) to avoid duplicating work
-    * If there isn't one already, please create one so that others know you're working on this
+1. Check that there isn't already an issue about your idea in the [nf-core/rnafusion issues](https://github.com/nf-core/rnafusion/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this
 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rnafusion repository](https://github.com/nf-core/rnafusion) to your GitHub account
 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
 4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
@@ -49,9 +48,9 @@ These tests are run both with the latest available version of `Nextflow` and als
 
 :warning: Only in the unlikely and regretful event of a release happening with a bug.
 
-* On your own fork, make a new branch `patch` based on `upstream/master`.
-* Fix the bug, and bump version (X.Y.Z+1).
-* A PR should be made on `master` from patch to directly this particular bug.
+- On your own fork, make a new branch `patch` based on `upstream/master`.
+- Fix the bug, and bump version (X.Y.Z+1).
+- A PR should be made on `master` from patch to directly this particular bug.
 
 ## Getting help
 
@@ -73,7 +72,7 @@ If you wish to contribute a new step, please use the following coding standards:
 6. Add sanity checks and validation for all relevant parameters.
 7. Perform local tests to validate that the new code works as expected.
 8. If applicable, add a new test command in `.github/workflow/ci.yml`.
-9. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module.
+9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module.
 10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`.
 
 ### Default values
@@ -92,8 +91,8 @@ The process resources can be passed on to the tool dynamically within the proces
 
 Please use the following naming schemes, to make it easy to understand what is going where.
 
-* initial process channel: `ch_output_from_<process>`
-* intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
+- initial process channel: `ch_output_from_<process>`
+- intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
 
 ### Nextflow version bumping
 
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index ca9d2123..a172d421 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -2,7 +2,6 @@ name: Bug report
 description: Report something that is broken or incorrect
 labels: bug
 body:
-
   - type: markdown
     attributes:
       value: |
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 6bf2080a..b47b142e 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -16,10 +16,10 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
 
 - [ ] This comment contains a description of changes (with reason).
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
-    - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
-    - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
+  - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+  - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint`).
-- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker` --outdir <OUTDIR>`).
+- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
 - [ ] `CHANGELOG.md` is updated.
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index ad566b83..f346e847 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -18,7 +18,6 @@ jobs:
         # TODO nf-core: You can customise AWS full pipeline tests as required
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
-        
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index ae430044..de699573 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -10,9 +10,9 @@ jobs:
     if: github.repository == 'nf-core/rnafusion'
     runs-on: ubuntu-latest
     steps:
+      # Launch workflow using Tower CLI tool action
       - name: Launch workflow via tower
         uses: nf-core/tower-action@v3
-        
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 37b7dea8..8623dfc2 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -13,8 +13,7 @@ jobs:
       - name: Check PRs
         if: github.repository == 'nf-core/rnafusion'
         run: |
-          { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
-
+          "{ [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]"
 
       # If the above check failed, post a comment on the PR explaining the failure
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
@@ -43,4 +42,4 @@ jobs:
             Thanks again for your contribution!
           repo-token: ${{ secrets.GITHUB_TOKEN }}
           allow-repeats: false
-
+#
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 2eef1cc9..791ad5a0 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -16,18 +16,18 @@ jobs:
   test:
     name: Run pipeline with test data
     # Only run on push if this is the nf-core dev branch (merged PRs)
-    if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}
+    if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}"
     runs-on: ubuntu-latest
     strategy:
       matrix:
         # Nextflow versions
         include:
           # Test pipeline minimum Nextflow version
-          - NXF_VER: '21.10.3'
-            NXF_EDGE: ''
+          - NXF_VER: "21.10.3"
+            NXF_EDGE: ""
           # Test latest edge release of Nextflow
-          - NXF_VER: ''
-            NXF_EDGE: '1'
+          - NXF_VER: ""
+            NXF_EDGE: "1"
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
@@ -48,3 +48,5 @@ jobs:
         # Remember that you can parallelise this by using strategy.matrix
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results
+
+#
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index fda934c0..e9cf5de3 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -1,6 +1,7 @@
 name: nf-core linting
 # This workflow is triggered on pushes and PRs to the repository.
-# It runs the `nf-core lint` and markdown lint tests to ensure that the code meets the nf-core guidelines
+# It runs the `nf-core lint` and markdown lint tests to ensure
+# that the code meets the nf-core guidelines.
 on:
   push:
   pull_request:
@@ -8,42 +9,6 @@ on:
     types: [published]
 
 jobs:
-  Markdown:
-    runs-on: ubuntu-latest
-    steps:
-      - uses: actions/checkout@v2
-      - uses: actions/setup-node@v2
-      - name: Install markdownlint
-        run: npm install -g markdownlint-cli
-      - name: Run Markdownlint
-        run: markdownlint .
-
-      # If the above check failed, post a comment on the PR explaining the failure
-      - name: Post PR comment
-        if: failure()
-        uses: mshick/add-pr-comment@v1
-        with:
-          message: |
-            ## Markdown linting is failing
-
-            To keep the code consistent with lots of contributors, we run automated code consistency checks.
-            To fix this CI test, please run:
-
-            * Install `markdownlint-cli`
-                * On Mac: `brew install markdownlint-cli`
-                * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`)
-            * Fix the markdown errors
-                * Automatically: `markdownlint . --fix`
-                * Manually resolve anything left from `markdownlint .`
-
-            Once you push these changes the test should pass, and you can hide this comment :+1:
-
-            We highly recommend setting up markdownlint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help!
-
-            Thanks again for your contribution!
-          repo-token: ${{ secrets.GITHUB_TOKEN }}
-          allow-repeats: false
-
   EditorConfig:
     runs-on: ubuntu-latest
     steps:
@@ -55,49 +20,24 @@ jobs:
         run: npm install -g editorconfig-checker
 
       - name: Run ECLint check
-        run: editorconfig-checker -exclude README.md $(git ls-files | grep -v test)
+        run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile')
 
-  YAML:
+  Prettier:
     runs-on: ubuntu-latest
     steps:
-      - name: Checkout
-        uses: actions/checkout@master
-      - name: 'Yamllint'
-        uses: karancode/yamllint-github-action@master
-        with:
-          yamllint_file_or_dir: '.'
-          yamllint_config_filepath: '.yamllint.yml'
-
-      # If the above check failed, post a comment on the PR explaining the failure
-      - name: Post PR comment
-        if: failure()
-        uses: mshick/add-pr-comment@v1
-        with:
-          message: |
-            ## YAML linting is failing
-
-            To keep the code consistent with lots of contributors, we run automated code consistency checks.
-            To fix this CI test, please run:
-
-            * Install `yamllint`
-                * Install `yamllint` following [this](https://yamllint.readthedocs.io/en/stable/quickstart.html#installing-yamllint)
-                instructions or alternative install it in your [conda environment](https://anaconda.org/conda-forge/yamllint)
-            * Fix the markdown errors
-                * Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml") -c ./.yamllint.yml`
-                * Fix any reported errors in your YAML files
+      - uses: actions/checkout@v2
 
-            Once you push these changes the test should pass, and you can hide this comment :+1:
+      - uses: actions/setup-node@v2
 
-            We highly recommend setting up yaml-lint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help!
+      - name: Install Prettier
+        run: npm install -g prettier
 
-            Thanks again for your contribution!
-          repo-token: ${{ secrets.GITHUB_TOKEN }}
-          allow-repeats: false
+      - name: Run Prettier --check
+        run: prettier --check ${GITHUB_WORKSPACE}
 
   nf-core:
     runs-on: ubuntu-latest
     steps:
-
       - name: Check out pipeline code
         uses: actions/checkout@v2
 
@@ -110,8 +50,8 @@ jobs:
 
       - uses: actions/setup-python@v1
         with:
-          python-version: '3.6'
-          architecture: 'x64'
+          python-version: "3.6"
+          architecture: "x64"
 
       - name: Install dependencies
         run: |
@@ -139,3 +79,4 @@ jobs:
             lint_results.md
             PR_number.txt
 
+#
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 44d72994..91c487a1 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -1,4 +1,3 @@
-
 name: nf-core linting comment
 # This workflow is triggered after the linting action is complete
 # It posts an automated comment to the PR, even if the PR is coming from a fork
@@ -27,4 +26,4 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
           path: linting-logs/lint_results.md
-
+#
diff --git a/.gitpod.yml b/.gitpod.yml
index b7d4cee1..c452ee93 100644
--- a/.gitpod.yml
+++ b/.gitpod.yml
@@ -2,13 +2,13 @@ image: nfcore/gitpod:latest
 
 vscode:
   extensions: # based on nf-core.nf-core-extensionpack
-    - codezombiech.gitignore                 # Language support for .gitignore files
+    - codezombiech.gitignore # Language support for .gitignore files
     # - cssho.vscode-svgviewer                 # SVG viewer
-    - davidanson.vscode-markdownlint         # Markdown/CommonMark linting and style checking for Visual Studio Code
-    - eamodio.gitlens                        # Quickly glimpse into whom, why, and when a line or code block was changed
-    - EditorConfig.EditorConfig              # override user/workspace settings with settings found in .editorconfig files
-    - Gruntfuggly.todo-tree                  # Display TODO and FIXME in a tree view in the activity bar
-    - mechatroner.rainbow-csv                # Highlight columns in csv files in different colors
+    - davidanson.vscode-markdownlint # Markdown/CommonMark linting and style checking for Visual Studio Code
+    - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed
+    - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files
+    - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar
+    - mechatroner.rainbow-csv # Highlight columns in csv files in different colors
     # - nextflow.nextflow                      # Nextflow syntax highlighting
-    - oderwat.indent-rainbow                 # Highlight indentation level
-    - streetsidesoftware.code-spell-checker  # Spelling checker for source code
+    - oderwat.indent-rainbow # Highlight indentation level
+    - streetsidesoftware.code-spell-checker # Spelling checker for source code
diff --git a/.markdownlint.yml b/.markdownlint.yml
deleted file mode 100644
index 9e605fcf..00000000
--- a/.markdownlint.yml
+++ /dev/null
@@ -1,14 +0,0 @@
-# Markdownlint configuration file
-default: true
-line-length: false
-ul-indent:
-    indent: 4
-no-duplicate-header:
-    siblings_only: true
-no-inline-html:
-    allowed_elements:
-        - img
-        - p
-        - kbd
-        - details
-        - summary
diff --git a/.prettierrc.yml b/.prettierrc.yml
new file mode 100644
index 00000000..c81f9a76
--- /dev/null
+++ b/.prettierrc.yml
@@ -0,0 +1 @@
+printWidth: 120
diff --git a/.yamllint.yml b/.yamllint.yml
deleted file mode 100644
index d466deec..00000000
--- a/.yamllint.yml
+++ /dev/null
@@ -1,6 +0,0 @@
-extends: default
-
-rules:
-    document-start: disable
-    line-length: disable
-    truthy: disable
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 01d65559..cef34b91 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -11,6 +11,8 @@ Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.r
 
 ### `Fixed`
 
+- Clarified conda usage and added an installation tutorial for Singularity since the one on Syllabs' website uses an outdate version of GO Compiler
+
 ### `Dependencies`
 
 ### `Deprecated`
diff --git a/CITATIONS.md b/CITATIONS.md
index 702b64c9..b298989b 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -10,23 +10,26 @@
 
 ## Pipeline tools
 
-* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
+- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
 
-* [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
-    > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
+- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
+  > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
 
 ## Software packaging/containerisation tools
 
-* [Anaconda](https://anaconda.com)
-    > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web.
+- [Anaconda](https://anaconda.com)
 
-* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/)
-    > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506.
+  > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web.
 
-* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/)
-    > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671.
+- [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/)
 
-* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241)
+  > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506.
 
-* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/)
-    > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675.
+- [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/)
+
+  > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671.
+
+- [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241)
+
+- [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/)
+  > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675.
diff --git a/README.md b/README.md
index 5beef0b2..465054ef 100644
--- a/README.md
+++ b/README.md
@@ -1,4 +1,4 @@
-# ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_light.png#gh-light-mode-only) ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_dark.png#gh-dark-mode-only)
+# ![nf-core/rnafusion](docs/images/nf-core/rnafusion_logo_light.png#gh-light-mode-only) ![nf-core/rnafusion](docs/images/nf-core/rnafusion_logo_dark.png#gh-dark-mode-only)
 
 [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+CI%22)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)
@@ -17,11 +17,13 @@
 ## Introduction
 
 <!-- TODO nf-core: Write a 1-2 sentence summary of what data the pipeline is for and what it does -->
+
 **nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for Nextflow rnafusion analysis pipeline, part of the nf-core community..
 
 The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
 
 <!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
+
 On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).
 
 ## Pipeline summary
@@ -35,28 +37,28 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`)
 
-2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_
+2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_.
 
 3. Download the pipeline and test it on a minimal dataset with a single command:
 
-    ```console
-    nextflow run nf-core/rnafusion -profile test,YOURPROFILE --outdir <OUTDIR>
-    ```
+   ```console
+   nextflow run nf-core/rnafusion -profile test,YOURPROFILE --outdir <OUTDIR>
+   ```
 
-    Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
+   Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
 
-    > * The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
-    > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
-    > * If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
-    > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
+   > - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
+   > - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
+   > - If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
+   > - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
 
 4. Start running your own analysis!
 
-    <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
+   <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
 
-    ```console
-    nextflow run nf-core/rnafusion --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
-    ```
+   ```console
+   nextflow run nf-core/rnafusion --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
+   ```
 
 ## Documentation
 
@@ -82,6 +84,7 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 <!-- If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
 
 <!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
+
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
 
 You can cite the `nf-core` publication as follows:
diff --git a/assets/email_template.html b/assets/email_template.html
index efcd0eaa..306f8e4f 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -1,53 +1,111 @@
 <html>
-<head>
-  <meta charset="utf-8">
-  <meta http-equiv="X-UA-Compatible" content="IE=edge">
-  <meta name="viewport" content="width=device-width, initial-scale=1">
+  <head>
+    <meta charset="utf-8" />
+    <meta http-equiv="X-UA-Compatible" content="IE=edge" />
+    <meta name="viewport" content="width=device-width, initial-scale=1" />
 
-  <meta name="description" content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community.">
-  <title>nf-core/rnafusion Pipeline Report</title>
-</head>
-<body>
-<div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto;">
+    <!-- prettier-ignore -->
+    <meta name="description" content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community." />
+    <title>nf-core/rnafusion Pipeline Report</title>
+  </head>
+  <body>
+    <div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto">
+      <img src="cid:nfcorepipelinelogo" />
 
-<img src="cid:nfcorepipelinelogo">
+      <h1>nf-core/rnafusion v${version}</h1>
+      <h2>Run Name: $runName</h2>
 
-<h1>nf-core/rnafusion v${version}</h1>
-<h2>Run Name: $runName</h2>
-
-<% if (!success){
-    out << """
-    <div style="color: #a94442; background-color: #f2dede; border-color: #ebccd1; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
-        <h4 style="margin-top:0; color: inherit;">nf-core/rnafusion execution completed unsuccessfully!</h4>
+      <% if (!success){ out << """
+      <div
+        style="
+          color: #a94442;
+          background-color: #f2dede;
+          border-color: #ebccd1;
+          padding: 15px;
+          margin-bottom: 20px;
+          border: 1px solid transparent;
+          border-radius: 4px;
+        "
+      >
+        <h4 style="margin-top: 0; color: inherit">nf-core/rnafusion execution completed unsuccessfully!</h4>
         <p>The exit status of the task that caused the workflow execution to fail was: <code>$exitStatus</code>.</p>
         <p>The full error message was:</p>
-        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0;">${errorReport}</pre>
-    </div>
-    """
-} else {
-    out << """
-    <div style="color: #3c763d; background-color: #dff0d8; border-color: #d6e9c6; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
+        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0">${errorReport}</pre>
+      </div>
+      """ } else { out << """
+      <div
+        style="
+          color: #3c763d;
+          background-color: #dff0d8;
+          border-color: #d6e9c6;
+          padding: 15px;
+          margin-bottom: 20px;
+          border: 1px solid transparent;
+          border-radius: 4px;
+        "
+      >
         nf-core/rnafusion execution completed successfully!
-    </div>
-    """
-}
-%>
+      </div>
+      """ } %>
 
-<p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
-<p>The command used to launch the workflow was as follows:</p>
-<pre style="white-space: pre-wrap; overflow: visible; background-color: #ededed; padding: 15px; border-radius: 4px; margin-bottom:30px;">$commandLine</pre>
+      <p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
+      <p>The command used to launch the workflow was as follows:</p>
+      <pre
+        style="
+          white-space: pre-wrap;
+          overflow: visible;
+          background-color: #ededed;
+          padding: 15px;
+          border-radius: 4px;
+          margin-bottom: 30px;
+        "
+      >
+$commandLine</pre
+      >
 
-<h3>Pipeline Configuration:</h3>
-<table style="width:100%; max-width:100%; border-spacing: 0; border-collapse: collapse; border:0; margin-bottom: 30px;">
-    <tbody style="border-bottom: 1px solid #ddd;">
-        <% out << summary.collect{ k,v -> "<tr><th style='text-align:left; padding: 8px 0; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'>$k</th><td style='text-align:left; padding: 8px; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'><pre style='white-space: pre-wrap; overflow: visible;'>$v</pre></td></tr>" }.join("\n") %>
-    </tbody>
-</table>
+      <h3>Pipeline Configuration:</h3>
+      <table
+        style="
+          width: 100%;
+          max-width: 100%;
+          border-spacing: 0;
+          border-collapse: collapse;
+          border: 0;
+          margin-bottom: 30px;
+        "
+      >
+        <tbody style="border-bottom: 1px solid #ddd">
+          <% out << summary.collect{ k,v -> "
+          <tr>
+            <th
+              style="
+                text-align: left;
+                padding: 8px 0;
+                line-height: 1.42857143;
+                vertical-align: top;
+                border-top: 1px solid #ddd;
+              "
+            >
+              $k
+            </th>
+            <td
+              style="
+                text-align: left;
+                padding: 8px;
+                line-height: 1.42857143;
+                vertical-align: top;
+                border-top: 1px solid #ddd;
+              "
+            >
+              <pre style="white-space: pre-wrap; overflow: visible">$v</pre>
+            </td>
+          </tr>
+          " }.join("\n") %>
+        </tbody>
+      </table>
 
-<p>nf-core/rnafusion</p>
-<p><a href="https://github.com/nf-core/rnafusion">https://github.com/nf-core/rnafusion</a></p>
-
-</div>
-
-</body>
+      <p>nf-core/rnafusion</p>
+      <p><a href="https://github.com/nf-core/rnafusion">https://github.com/nf-core/rnafusion</a></p>
+    </div>
+  </body>
 </html>
diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml
deleted file mode 100644
index 611287cc..00000000
--- a/assets/multiqc_config.yaml
+++ /dev/null
@@ -1,11 +0,0 @@
-report_comment: >
-    This report has been generated by the <a href="https://github.com/nf-core/rnafusion" target="_blank">nf-core/rnafusion</a>
-    analysis pipeline. For information about how to interpret these results, please see the
-    <a href="https://nf-co.re/rnafusion" target="_blank">documentation</a>.
-report_section_order:
-    software_versions:
-        order: -1000
-    nf-core-rnafusion-summary:
-        order: -1001
-
-export_plots: true
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
new file mode 100644
index 00000000..0ffd3e5e
--- /dev/null
+++ b/assets/multiqc_config.yml
@@ -0,0 +1,11 @@
+report_comment: >
+  This report has been generated by the <a href="https://github.com/nf-core/rnafusion" target="_blank">nf-core/rnafusion</a>
+  analysis pipeline. For information about how to interpret these results, please see the
+  <a href="https://nf-co.re/rnafusion" target="_blank">documentation</a>.
+report_section_order:
+  software_versions:
+    order: -1000
+  "nf-core-rnafusion-summary":
+    order: -1001
+
+export_plots: true
diff --git a/assets/schema_input.json b/assets/schema_input.json
index 435d174a..aff11de1 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -31,9 +31,6 @@
                 ]
             }
         },
-        "required": [
-            "sample",
-            "fastq_1"
-        ]
+        "required": ["sample", "fastq_1"]
     }
 }
diff --git a/docs/README.md b/docs/README.md
index 08dec85e..0c1d417d 100644
--- a/docs/README.md
+++ b/docs/README.md
@@ -2,9 +2,9 @@
 
 The nf-core/rnafusion documentation is split into the following pages:
 
-* [Usage](usage.md)
-    * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
-* [Output](output.md)
-    * An overview of the different results produced by the pipeline and how to interpret them.
+- [Usage](usage.md)
+  - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
+- [Output](output.md)
+  - An overview of the different results produced by the pipeline and how to interpret them.
 
 You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re)
diff --git a/docs/output.md b/docs/output.md
index 06979c38..dc6e6d48 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -12,18 +12,18 @@ The directories listed below will be created in the results directory after the
 
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
-* [FastQC](#fastqc) - Raw read QC
-* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
-* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
+- [FastQC](#fastqc) - Raw read QC
+- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
+- [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ### FastQC
 
 <details markdown="1">
 <summary>Output files</summary>
 
-* `fastqc/`
-    * `*_fastqc.html`: FastQC report containing quality metrics.
-    * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
+- `fastqc/`
+  - `*_fastqc.html`: FastQC report containing quality metrics.
+  - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
 </details>
 
@@ -42,10 +42,10 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 <details markdown="1">
 <summary>Output files</summary>
 
-* `multiqc/`
-    * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
-    * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
-    * `multiqc_plots/`: directory containing static images from the report in various formats.
+- `multiqc/`
+  - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
+  - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
+  - `multiqc_plots/`: directory containing static images from the report in various formats.
 
 </details>
 
@@ -58,10 +58,10 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ
 <details markdown="1">
 <summary>Output files</summary>
 
-* `pipeline_info/`
-    * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
-    * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
-    * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
+- `pipeline_info/`
+  - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
+  - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
+  - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
 
 </details>
 
diff --git a/docs/usage.md b/docs/usage.md
index d1e3eb21..f398e3b4 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -44,11 +44,11 @@ TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,
 TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,
 ```
 
-| Column         | Description                                                                                                                                                                            |
-|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
-| `sample`       | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). |
-| `fastq_1`      | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
-| `fastq_2`      | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
+| Column    | Description                                                                                                                                                                            |
+| --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
+| `sample`  | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). |
+| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
+| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
 
 An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline.
 
@@ -57,7 +57,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p
 The typical command for running the pipeline is as follows:
 
 ```console
-nextflow run nf-core/rnafusion --input samplesheet.csv  --outdir <OUTDIR> --genome GRCh37 -profile docker
+nextflow run nf-core/rnafusion --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile docker
 ```
 
 This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
@@ -65,9 +65,9 @@ This will launch the pipeline with the `docker` configuration profile. See below
 Note that the pipeline will create the following files in your working directory:
 
 ```console
-work            # Directory containing the nextflow working files
-results         # Finished results (configurable, see below)
-.nextflow_log   # Log file from Nextflow
+work                # Directory containing the nextflow working files
+<OUTIDR>            # Finished results in specified location (defined with --outdir)
+.nextflow_log       # Log file from Nextflow
 # Other nextflow hidden files, eg. history of pipeline runs and old logs.
 ```
 
@@ -106,25 +106,25 @@ They are loaded in sequence, so later profiles can overwrite earlier profiles.
 
 If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended.
 
-* `docker`
-    * A generic configuration profile to be used with [Docker](https://docker.com/)
-* `singularity`
-    * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
-* `podman`
-    * A generic configuration profile to be used with [Podman](https://podman.io/)
-* `shifter`
-    * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
-* `charliecloud`
-    * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
-* `conda`
-    * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
-* `test`
-    * A profile with a complete configuration for automated testing
-    * Includes links to test data so needs no other parameters
+- `docker`
+  - A generic configuration profile to be used with [Docker](https://docker.com/)
+- `singularity`
+  - A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
+- `podman`
+  - A generic configuration profile to be used with [Podman](https://podman.io/)
+- `shifter`
+  - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
+- `charliecloud`
+  - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
+- `conda`
+  - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
+- `test`
+  - A profile with a complete configuration for automated testing
+  - Includes links to test data so needs no other parameters
 
 ### `-resume`
 
-Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously.
+Specify this when restarting a pipeline. Nextflow will use cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. For input to be considered the same, not only the names must be identical but the files' contents as well. For more info about this parameter, see [this blog post](https://www.nextflow.io/blog/2019/demystifying-nextflow-resume.html).
 
 You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names.
 
@@ -186,6 +186,7 @@ process {
 ```
 
 > **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden.
+>
 > If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
 
 ### Updating containers
@@ -196,35 +197,35 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
 2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
 3. Create the custom config accordingly:
 
-    * For Docker:
-
-        ```nextflow
-        process {
-            withName: PANGOLIN {
-                container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0'
-            }
-        }
-        ```
-
-    * For Singularity:
-
-        ```nextflow
-        process {
-            withName: PANGOLIN {
-                container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0'
-            }
-        }
-        ```
-
-    * For Conda:
-
-        ```nextflow
-        process {
-            withName: PANGOLIN {
-                conda = 'bioconda::pangolin=3.0.5'
-            }
-        }
-        ```
+   - For Docker:
+
+     ```nextflow
+     process {
+         withName: PANGOLIN {
+             container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0'
+         }
+     }
+     ```
+
+   - For Singularity:
+
+     ```nextflow
+     process {
+         withName: PANGOLIN {
+             container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0'
+         }
+     }
+     ```
+
+   - For Conda:
+
+     ```nextflow
+     process {
+         withName: PANGOLIN {
+             conda = 'bioconda::pangolin=3.0.5'
+         }
+     }
+     ```
 
 > **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch.
 
diff --git a/modules.json b/modules.json
index 0e469950..a7159f29 100644
--- a/modules.json
+++ b/modules.json
@@ -4,14 +4,14 @@
     "repos": {
         "nf-core/modules": {
             "custom/dumpsoftwareversions": {
-                "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "fastqc": {
-                "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "multiqc": {
-                "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
index 934bb467..327d5100 100644
--- a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
@@ -15,6 +15,9 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     path "software_versions_mqc.yml", emit: mqc_yml
     path "versions.yml"             , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def args = task.ext.args ?: ''
     template 'dumpsoftwareversions.py'
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
index 5b5b8a60..60b546a0 100644
--- a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
+++ b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
@@ -8,7 +8,7 @@ tools:
       description: Custom module used to dump software versions within the nf-core pipeline template
       homepage: https://github.com/nf-core/tools
       documentation: https://github.com/nf-core/tools
-      licence: ['MIT']
+      licence: ["MIT"]
 input:
   - versions:
       type: file
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
index d250eca0..ed6b8c50 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -15,6 +15,9 @@ process FASTQC {
     tuple val(meta), path("*.zip") , emit: zip
     path  "versions.yml"           , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def args = task.ext.args ?: ''
     // Add soft-links to original FastQs for consistent naming in pipeline
diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml
index b09553a3..4da5bb5a 100644
--- a/modules/nf-core/modules/fastqc/meta.yml
+++ b/modules/nf-core/modules/fastqc/meta.yml
@@ -1,52 +1,52 @@
 name: fastqc
 description: Run FastQC on sequenced reads
 keywords:
-    - quality control
-    - qc
-    - adapters
-    - fastq
+  - quality control
+  - qc
+  - adapters
+  - fastq
 tools:
-    - fastqc:
-        description: |
-            FastQC gives general quality metrics about your reads.
-            It provides information about the quality score distribution
-            across your reads, the per base sequence content (%A/C/G/T).
-            You get information about adapter contamination and other
-            overrepresented sequences.
-        homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
-        documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
-        licence: ['GPL-2.0-only']
+  - fastqc:
+      description: |
+        FastQC gives general quality metrics about your reads.
+        It provides information about the quality score distribution
+        across your reads, the per base sequence content (%A/C/G/T).
+        You get information about adapter contamination and other
+        overrepresented sequences.
+      homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
+      documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
+      licence: ["GPL-2.0-only"]
 input:
-    - meta:
-        type: map
-        description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-    - reads:
-        type: file
-        description: |
-            List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-            respectively.
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
 output:
-    - meta:
-        type: map
-        description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-    - html:
-        type: file
-        description: FastQC report
-        pattern: "*_{fastqc.html}"
-    - zip:
-        type: file
-        description: FastQC report archive
-        pattern: "*_{fastqc.zip}"
-    - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - html:
+      type: file
+      description: FastQC report
+      pattern: "*_{fastqc.html}"
+  - zip:
+      type: file
+      description: FastQC report archive
+      pattern: "*_{fastqc.zip}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 authors:
-    - "@drpatelh"
-    - "@grst"
-    - "@ewels"
-    - "@FelixKrueger"
+  - "@drpatelh"
+  - "@grst"
+  - "@ewels"
+  - "@FelixKrueger"
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index 3dceb162..1264aac1 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -1,10 +1,10 @@
 process MULTIQC {
     label 'process_medium'
 
-    conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null)
+    conda (params.enable_conda ? 'bioconda::multiqc=1.12' : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.12--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.12--pyhdfd78af_0' }"
 
     input:
     path multiqc_files
@@ -15,6 +15,9 @@ process MULTIQC {
     path "*_plots"             , optional:true, emit: plots
     path "versions.yml"        , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     def args = task.ext.args ?: ''
     """
diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml
index 63c75a45..6fa891ef 100644
--- a/modules/nf-core/modules/multiqc/meta.yml
+++ b/modules/nf-core/modules/multiqc/meta.yml
@@ -1,40 +1,40 @@
 name: MultiQC
 description: Aggregate results from bioinformatics analyses across many samples into a single report
 keywords:
-    - QC
-    - bioinformatics tools
-    - Beautiful stand-alone HTML report
+  - QC
+  - bioinformatics tools
+  - Beautiful stand-alone HTML report
 tools:
-    - multiqc:
-        description: |
-            MultiQC searches a given directory for analysis logs and compiles a HTML report.
-            It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
-        homepage: https://multiqc.info/
-        documentation: https://multiqc.info/docs/
-        licence: ['GPL-3.0-or-later']
+  - multiqc:
+      description: |
+        MultiQC searches a given directory for analysis logs and compiles a HTML report.
+        It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
+      homepage: https://multiqc.info/
+      documentation: https://multiqc.info/docs/
+      licence: ["GPL-3.0-or-later"]
 input:
-    - multiqc_files:
-        type: file
-        description: |
-            List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+  - multiqc_files:
+      type: file
+      description: |
+        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
 output:
-    - report:
-        type: file
-        description: MultiQC report file
-        pattern: "multiqc_report.html"
-    - data:
-        type: dir
-        description: MultiQC data dir
-        pattern: "multiqc_data"
-    - plots:
-        type: file
-        description: Plots created by MultiQC
-        pattern: "*_data"
-    - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+  - report:
+      type: file
+      description: MultiQC report file
+      pattern: "multiqc_report.html"
+  - data:
+      type: dir
+      description: MultiQC data dir
+      pattern: "multiqc_data"
+  - plots:
+      type: file
+      description: Plots created by MultiQC
+      pattern: "*_data"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 authors:
-    - "@abhi18av"
-    - "@bunop"
-    - "@drpatelh"
+  - "@abhi18av"
+  - "@bunop"
+  - "@drpatelh"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 99a42236..e91cc322 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -10,10 +10,7 @@
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": [
-                "input",
-                "outdir"
-            ],
+            "required": ["input", "outdir"],
             "properties": {
                 "input": {
                     "type": "string",
@@ -185,14 +182,7 @@
                     "description": "Method used to save pipeline results to output directory.",
                     "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
                     "fa_icon": "fas fa-copy",
-                    "enum": [
-                        "symlink",
-                        "rellink",
-                        "link",
-                        "copy",
-                        "copyNoFollow",
-                        "move"
-                    ],
+                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
                     "hidden": true
                 },
                 "email_on_fail": {
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index acc8b85f..8914d885 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -23,7 +23,7 @@ if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input sample
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-ch_multiqc_config        = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true)
+ch_multiqc_config        = file("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
 ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
 
 /*

From b6e1b3bcb5997570a090371eeb6bbd09eb1638b8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 23 Mar 2022 17:43:45 +0100
Subject: [PATCH 223/359] update docs

---
 CITATIONS.md                                  |   1 +
 README.md                                     |   7 +-
 conf/modules.config                           |   2 -
 docs/output.md                                | 325 ++++++++++++++----
 docs/usage.md                                 |  19 +-
 subworkflows/local/arriba_workflow.nf         |   4 -
 subworkflows/local/fusioncatcher_workflow.nf  |   4 -
 .../local/fusioninspector_workflow.nf         |   6 -
 subworkflows/local/fusionreport_workflow.nf   |   4 -
 subworkflows/local/pizzly_workflow.nf         |   4 -
 subworkflows/local/squid_workflow.nf          |   4 -
 subworkflows/local/starfusion_workflow.nf     |   6 +-
 12 files changed, 283 insertions(+), 103 deletions(-)

diff --git a/CITATIONS.md b/CITATIONS.md
index 7c1a47f3..07feb112 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -23,6 +23,7 @@
     > Nicorici D, Satalan M, Edgren H, Kangaspeska S, Murumagi A, Kallioniemi O, Virtanen S, Kilkku O. FusionCatcher – a tool for finding somatic fusion genes in paired-end RNA-sequencing data. BioRxiv, 2014 Nov. doi: 10.1101/011650.
 
 * [Fusion-report](https://github.com/matq007/fusion-report)
+    > Proks M, Genomic Profiling of a Comprehensive Nation-wide Collection of Childhood Solid Tumors, Master Thesis, Supervisors: Grøntved L, Díaz de Ståhl T, Nistér M, Ewels P, Garcia MU, Juhos S, University of Southern Denmark, 2019, unpublished.
 
 * [Kallisto](https://pachterlab.github.io/kallisto/)
     > Bray NL, Pimentel H, Melsted P, Pachter L. Near-optimal probabilistic RNA-seq quantification. Nature Biotechnology 2016 Apr. 34, 525–527. doi:10.1038/nbt.3519. PMID: 27043002.
diff --git a/README.md b/README.md
index 2e4498b3..c4ab04e4 100644
--- a/README.md
+++ b/README.md
@@ -4,7 +4,6 @@
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)
 [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)
 [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3946477)
-<!-- TODO update zenodo after release -->
 
 [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
@@ -21,6 +20,11 @@
 
 The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
 
+
+> **IMPORTANT: conda is not supported currently.** Run with singularity or docker.
+
+> GRCh38 is the only supported reference
+
 | Tool                                                                      |  Single-end reads  |  Version |
 | ------------------------------------------------------------------------- | :----------------: | :------: |
 | [Arriba](https://github.com/suhrig/arriba)                                |         :x:        |  `2.1.0` |
@@ -29,7 +33,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 | [Pizzly](https://github.com/pmelsted/pizzly)                              |         :x:        | `0.37.3` |
 | [Squid](https://github.com/Kingsford-Group/squid)                         |         :x:        |   `1.5`  |
 | [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: | `1.10.1` |
-<!-- TODO fusion-report: check version after release -->
 
 ## Pipeline summary
 
diff --git a/conf/modules.config b/conf/modules.config
index 2f258012..c567c539 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -244,5 +244,3 @@ process {
         ]
     }
 }
-
-
diff --git a/docs/output.md b/docs/output.md
index a83b5b99..c38a2a7a 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -12,75 +12,66 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 * [Download and build references](#references) - Build references needed to run the rest of the pipeline
 * [STAR](#star) - Alignment for arriba, squid and STAR-fusion
-* [cat](#cat) - Concatenated fastq files per sample ID
+* [Cat](#cat) - Concatenated fastq files per sample ID
 * [Arriba](#arriba) - Arriba fusion detection
 * [Pizzly](#pizzly) - Pizzly fusion detection
 * [Squid](#squid) - Squid fusion detection
 * [STAR-fusion](#starfusion) - STAR-fusion fusion detection
 * [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
+* [Samtools](#samtools) - SAM/BAM file manipulation
 * [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report
 * [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman and FusionGBD databases
-* [FusionInspectior](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
-* [FastQC](#fastqc) - Raw read QC
+* [FusionInspector](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
+* [Qualimap](#qualimap) - quality control of alignment
+* [Picard](#picard) - collect metrics
+* [FastQC](#fastqc) - Raw read quality control
 * [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
 * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 
-
-<!-- 1. Input samplesheet check
-2. Concatenate fastq files per sample
-3. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-4. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
-5. Arriba subworkflow
-    * STAR alignment
-    * Samtool sort
-    * Samtool index
-    * [Arriba](https://github.com/suhrig/arriba) fusion detection
-    * [Arriba](https://github.com/suhrig/arriba) visualisation
-6. Pizzly subworkflow
-    * [Kallisto](https://pachterlab.github.io/kallisto/) quantification
-    * [Pizzly](https://github.com/pmelsted/pizzly) fusion detection
-7. Squid subworkflow
-    * [STAR](https://github.com/alexdobin/STAR) alignment
-    * [Samtools view](http://www.htslib.org/): convert sam output from STAR to bam
-    * [Samtools sort](http://www.htslib.org/): bam output from STAR
-    * [Squid](https://github.com/Kingsford-Group/squid) fusion detection
-    * [Squid](https://github.com/Kingsford-Group/squid) annotate
-8. STAR-fusion subworkflow
-    * [STAR](https://github.com/alexdobin/STAR) alignment
-    * [STAR-fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
-9. Fusioncatcher subworkflow
-    * [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
-10. Fusion-report subworkflow
-    * Merge all fusions detected by the different tools
-    * [Fusion-report](https://github.com/matq007/fusion-report)
-11. FusionInspector subworkflow
-    * [FusionInspector](https://github.com/FusionInspector/FusionInspector) -->
-
-<!-- TODO details on each step -->
 ### Download and build references
 
 <details markdown="1">
 <summary>Output files</summary>
 
- * `genomes_base/`
+* `genomes_base/`
     * `arriba`
-
-<!-- TODO complete list of reference output files -->
-
+        * `blacklist_hg38_GRCh38_v2.1.0.tsv.gz`
+        * `protein_domains_hg38_GRCh38_v2.1.0.gff3`
+        * `cytobands_hg38_GRCh38_v2.1.0.tsv`
+    * `ensembl`
+        * `Homo_sapiens.GRCh38.{ensembl_version}.all.fa`
+        * `Homo_sapiens.GRCh38.{ensembl_version}.cdna.all.fa.gz`
+        * `Homo_sapiens.GRCh38.{ensembl_version}.gtf`
+        * `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf`
+        * `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf.refflat`
+    * `fusioncatcher`
+        * `human_v<version>` - dir with all references for fusioncatcher
+    * `fusion_report_db`
+        * `cosmic.db`
+        * `fusiongdb.db`
+        * `fusiongdb2.db`
+        * `mitelman.db`
+    * `pizzly`
+        * `kallisto` - file containing the kallisto index
+    * `star` - dir with STAR index
+    * `starfusion`
+        * files and dirs used to build the index
+        * `ctat_genome_lib_build_dir` - dir containing the index
+
+(Only files or folders used by the pipeline are mentioned explicitly.)
+</details>
 
 ### STAR
 
-<details markdown="1">
-<summary>Output files</summary>
-
-<!-- TODO complete list of output files -->
+STAR is used to align to genome reference
 
 STAR is run 3 times:
 
 For arriba with the parameters:
 
->`--readFilesCommand zcat \
+```bash
+--readFilesCommand zcat \
 --outSAMtype BAM Unsorted \
 --outSAMunmapped Within \
 --outBAMcompression 0 \
@@ -95,10 +86,13 @@ For arriba with the parameters:
 --chimScoreJunctionNonGTAG 0 \
 --chimScoreSeparation 1 \
 --chimSegmentReadGapMax 3 \
---chimMultimapNmax 50`
+--chimMultimapNmax 50
+```
 
 For squid with the parameters:
->`--twopassMode Basic \
+
+```bash
+--twopassMode Basic \
 --chimOutType SeparateSAMold \
 --chimSegmentMin 20 \
 --chimJunctionOverhangMin 12 \
@@ -106,10 +100,13 @@ For squid with the parameters:
 --outReadsUnmapped Fastx \
 --outSAMstrandField intronMotif \
 --outSAMtype BAM SortedByCoordinate \
---readFilesCommand zcat`
+--readFilesCommand zcat
+```
 
 For STAR-fusion with the parameters:
->`--twopassMode Basic \
+
+```bash
+--twopassMode Basic \
 --outReadsUnmapped None \
 --readFilesCommand zcat \
 --outSAMstrandField intronMotif \
@@ -130,20 +127,230 @@ For STAR-fusion with the parameters:
 --alignInsertionFlush Right \
 --alignSplicedMateMapLminOverLmate 0 \
 --alignSplicedMateMapLmin 30 \
---chimOutType Junctions`
+--chimOutType Junctions
+```
 
+> STAR_FOR_STARFUSION uses `${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf` whereas STAR_FOR_ARRIBA and STAR_FOR_SQUID use `${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf`
 
+<details markdown="1">
+<summary>Output files</summary>
 
-Alignment for arriba, squid and STAR-fusion
-* [cat](#cat) - Concatenated fastq files per sample ID
-* [Arriba](#arriba) - Arriba fusion detection
-* [Pizzly](#pizzly) - Pizzly fusion detection
-* [Squid](#squid) - Squid fusion detection
-* [STAR-fusion](#starfusion) - STAR-fusion fusion detection
-* [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
-* [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report
-* [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman and FusionGBD databases
-* [FusionInspectior](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
+* `star_for_<tool>`
+    * **Common**
+    * `<sample_id>.Log.final.out`
+    * `<sample_id>.Log.progress.out`
+    * `<sample_id>.SJ.out.tab`
+    * **For arriba:**
+    * `<sample_id>.Aligned.out.bam`
+    * **For squid:**
+    * `<sample_id>.Aligned.sortedByCoord.out.bam`
+    * `<sample_id>.Chimeric.out.sam`
+    * `<sample_id>.unmapped_1.fastq.gz`
+    * `<sample_id>.unmapped_2.fastq.gz`
+    * **For starfusion:**
+    * `<sample_id>.Aligned.sortedByCoord.out.bam`
+    * `<sample_id>.Chimeric.out.junction`
+</details>
+
+### Cat
+
+Cat is used to concatenate fastq files belonging to the same sample.
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `cat`
+    * `<sample_id>_1.merged.fastq.gz`
+    * `<sample_id>_2.merged.fastq.gz`
+
+</details>
+
+### Arriba
+
+Arriba is used for i) detect fusion and ii) output a PDF report for the fusions found (visualisation):
+#### Detection
+<details markdown="1">
+<summary>Output files</summary>
+
+* `arriba`
+    * `<sample_id>.arriba.fusions.tsv` - contains the identified fusions
+    * `<sample_id>.arriba.fusions.discarded.tsv`
+
+</details>
+
+#### Visualisation
+<details markdown="1">
+<summary>Output files</summary>
+
+* `arriba_visualisation`
+    * `<sample_id>.pdf`
+
+</details>
+
+### Pizzly
+
+The first step of the pizzly workflow is to run `kallisto quant`:
+
+#### Kallisto
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `kallisto`
+    * `<sample_id>.kallisto_quant.fusions.txt`
+
+</details>
+
+Pizzly refines kallisto output.
+
+#### Pizzly
+
+Pizzly uses the following arguments:
+
+```bash
+-k 31 \
+--align-score 2 \
+--insert-size 400 \
+--cache index.cache.txt
+```
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `pizzly`
+    * `<sample_id>.pizzly.txt` - contains the identified fusions
+    * `<sample_id>.pizzly.unfiltered.json`
+
+</details>
+
+
+### Squid
+
+Squid is run in two steps: i) fusion detection and ii) fusion annotation but the output is in a common `squid` directory.
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `squid`
+    * `<sample_id>.squid.fusions_sv.txt` - contains the identified fusions
+    * `<sample_id>.squid.fusions.annotated.txt`- contains the identified fusions annotatedvi
+
+</details>
+
+### STAR-fusion
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `starfusion`
+    * `<sample_id>.starfusion.fusion_predictions.tsv` - contains the identified fusions
+    * `<sample_id>.starfusion.abridged.tsv`
+    * `- contains the identified fusions.starfusion.abridged.coding_effect.tsv`
+
+</details>
+
+### FusionCatcher
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `fusioncatcher`
+    * `<sample_id>.fusioncatcher.fusion-genes.txt`
+    * `<sample_id>.fusioncatcher.summary.txt`
+    * `<sample_id>.fusioncatcher.log`
+</details>
+
+### Samtools
+
+#### Samtools view
+
+Samtools view is used to convert the chimeric SAM output from STAR_FOR_SQUID to BAM
+
+<details markdown="1">
+<summary>Output files</summary>
+
+*  `samtools_view_for_squid`
+    * `<sample_id>_chimeric.bam` - sorted BAM file
+
+</details>
+
+#### Samtools sort
+
+Samtools sort is used to sort BAM files from STAR_FOR_ARRIBA (for arriba visualisation) and the chimeric BAM from STAR_FOR_SQUID
+
+<details markdown="1">
+<summary>Output files</summary>
+
+*  `samtools_sort_for_<arriba/squid>`
+    * `<sample_id>(_chimeric)_sorted.bam` - sorted BAM file
+
+</details>
+
+#### Samtools index
+
+Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visualisation) and STAR_FOR_STARFUSION (for QC)
+
+<details markdown="1">
+<summary>Output files</summary>
+
+*  `samtools_for_<arriba/qc>`
+    * `<sample_id>.(Aligned.sortedByCoord).out.bam.bai` -
+
+</details>
+
+### Fusion-report
+
+<details markdown="1">
+<summary>Output files</summary>
+
+* `fusionreport`
+    * <sample_id>
+        * `<sample_id>.fusionreport.tsv`
+        * `<sample_id>.fusionreport_filtered.tsv`
+        * `index.html` - general report for all filtered fusions
+        * `<fusion>.html` - specific report for each filtered fusion
+
+</details>
+
+### FusionInspector
+
+<details markdown="1">
+<summary>Output files</summary>
+
+*  `fusioninspector`
+    * `<sample_id>.fusion_inspector_web.html` - visualisation report described in details [here](https://github.com/FusionInspector/FusionInspector/wiki/FusionInspector-Visualizations)
+    * `FusionInspector.log`
+    * `<sample_id>.FusionInspector.fusions.abridged.tsv`
+
+</details>
+
+### Qualimap
+
+<details markdown="1">
+<summary>Output files</summary>
+
+*  `qualimap`
+    * `qualimapReport.html` - HTML report
+    * `rnaseq_qc_results.txt` - TXT results
+    * `css` - dir for html style
+    * `images_qualimapReport`- dir for html images
+    * `raw_data_qualimapReport` - dir for html raw data
+
+
+</details>
+
+### Picard
+
+Picard CollectRnaMetrics and picard MarkDuplicates share the same outpur directory.
+<details markdown="1">
+<summary>Output files</summary>
+
+*  `picard`
+    * `<sample_id>.MarkDuplicates.metrics.txt` - metrics from CollectRnaMetrics
+    * `<sample_id>_rna_metrics.txt` - metrics from MarkDuplicates
+    * `<sample_id>.bam` - BAM file with marked duplicates
+
+</details>
 
 ### FastQC
 
diff --git a/docs/usage.md b/docs/usage.md
index 228accad..66d5e721 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -75,19 +75,19 @@ CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz
 
 ### Full samplesheet
 
-The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below.
+The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 4 columns to match those defined in the table below.
 
 A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice.
 
 ```console
-sample,fastq_1,fastq_2
-CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
-CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz
-CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz
-TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz,
-TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz,
-TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,
-TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,
+sample,fastq_1,fastq_2,strandedness
+CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz,forward
+CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz,forward
+CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz,forward
+TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz,,forward
+TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz,,forward
+TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,,forward
+TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,forward
 ```
 
 | Column         | Description                                                                                                                                                                            |
@@ -95,6 +95,7 @@ TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,
 | `sample`       | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). |
 | `fastq_1`      | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
 | `fastq_2`      | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
+| `strandedness` | Strandedness: forward or reverse.                                                                                                                                                      |
 
 An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline.
 
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index ddd1a139..27b714e5 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -1,7 +1,3 @@
-//
-// Check input samplesheet and get read channels
-//
-
 include { ARRIBA }                                      from '../../modules/nf-core/modules/arriba/main'
 include { ARRIBA_VISUALISATION }                        from '../../modules/local/arriba/visualisation/main'
 include { GET_PATH as GET_PATH_ARRIBA }                 from '../../modules/local/getpath/main'
diff --git a/subworkflows/local/fusioncatcher_workflow.nf b/subworkflows/local/fusioncatcher_workflow.nf
index f664d6b1..53ebdcb6 100644
--- a/subworkflows/local/fusioncatcher_workflow.nf
+++ b/subworkflows/local/fusioncatcher_workflow.nf
@@ -1,7 +1,3 @@
-//
-// Check input samplesheet and get read channels
-//
-
 include { FUSIONCATCHER }          from '../../modules/local/fusioncatcher/detect/main'
 include { GET_PATH }               from '../../modules/local/getpath/main'
 
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index aeb5bbb5..181f2514 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -1,7 +1,3 @@
-//
-// Check input samplesheet and get read channels
-//
-
 include { FUSIONINSPECTOR }                           from '../../modules/local/fusioninspector/main'
 
 
@@ -19,7 +15,5 @@ workflow FUSIONINSPECTOR_WORKFLOW {
 
     emit:
         versions        = ch_versions.ifEmpty(null)
-        // fusion_list     = FUSIONINSPECTOR.out.fusion_list
-
 }
 
diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index fd2e41df..085b08f3 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -1,7 +1,3 @@
-//
-// Check input samplesheet and get read channels
-//
-
 include { FUSIONREPORT }                           from '../../modules/local/fusionreport/detect/main'
 
 
diff --git a/subworkflows/local/pizzly_workflow.nf b/subworkflows/local/pizzly_workflow.nf
index 40b51dda..d1d5b07d 100644
--- a/subworkflows/local/pizzly_workflow.nf
+++ b/subworkflows/local/pizzly_workflow.nf
@@ -1,7 +1,3 @@
-//
-// Check input samplesheet and get read channels
-//
-
 include { KALLISTO_QUANT    }     from '../../modules/local/kallisto/quant/main'
 include { PIZZLY            }     from '../../modules/local/pizzly/detect/main'
 include { GET_PATH          }     from '../../modules/local/getpath/main'
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index c22d45e7..8d9f7d69 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -1,7 +1,3 @@
-//
-// Check input samplesheet and get read channels
-//
-
 include { GET_PATH }                                    from '../../modules/local/getpath/main'
 include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_SQUID }    from '../../modules/nf-core/modules/samtools/sort/main'
 include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_SQUID }    from '../../modules/nf-core/modules/samtools/view/main'
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index 19ad7cba..276e3ce7 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -1,10 +1,6 @@
-//
-// Check input samplesheet and get read channels
-//
-
 include { STAR_ALIGN as STAR_FOR_STARFUSION }    from '../../modules/nf-core/modules/star/align/main'
 include { STARFUSION }                           from '../../modules/local/starfusion/detect/main'
-include { GET_PATH }                         from '../../modules/local/getpath/main'
+include { GET_PATH }                             from '../../modules/local/getpath/main'
 
 
 workflow STARFUSION_WORKFLOW {

From 073bf7dd7e1d4dd6e50681a2df4fad82d897e449 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 24 Mar 2022 11:45:38 +0000
Subject: [PATCH 224/359] Template update for nf-core/tools version 2.3.2

---
 .github/PULL_REQUEST_TEMPLATE.md  | 1 -
 .github/workflows/awsfulltest.yml | 2 --
 .github/workflows/awstest.yml     | 2 --
 .gitpod.yml                       | 2 +-
 CHANGELOG.md                      | 2 --
 5 files changed, 1 insertion(+), 8 deletions(-)

diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index b47b142e..bc52a0a2 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -10,7 +10,6 @@ Remember that PRs should be made against the dev branch, unless you're preparing
 
 Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
 -->
-<!-- markdownlint-disable ul-indent -->
 
 ## PR checklist
 
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index f346e847..9b4362ea 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -22,8 +22,6 @@ jobs:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
-          pipeline: ${{ github.repository }}
-          revision: ${{ github.sha }}
           workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
           parameters: |
             {
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index de699573..933cbbf5 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -17,8 +17,6 @@ jobs:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
-          pipeline: ${{ github.repository }}
-          revision: ${{ github.sha }}
           workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
           parameters: |
             {
diff --git a/.gitpod.yml b/.gitpod.yml
index c452ee93..85d95ecc 100644
--- a/.gitpod.yml
+++ b/.gitpod.yml
@@ -4,7 +4,7 @@ vscode:
   extensions: # based on nf-core.nf-core-extensionpack
     - codezombiech.gitignore # Language support for .gitignore files
     # - cssho.vscode-svgviewer                 # SVG viewer
-    - davidanson.vscode-markdownlint # Markdown/CommonMark linting and style checking for Visual Studio Code
+    - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code
     - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed
     - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files
     - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar
diff --git a/CHANGELOG.md b/CHANGELOG.md
index cef34b91..01d65559 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -11,8 +11,6 @@ Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.r
 
 ### `Fixed`
 
-- Clarified conda usage and added an installation tutorial for Singularity since the one on Syllabs' website uses an outdate version of GO Compiler
-
 ### `Dependencies`
 
 ### `Deprecated`

From 14ec5f17c4856a58229db43e77396f9ad21f2575 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 10:32:06 +0100
Subject: [PATCH 225/359] remove line

---
 README.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/README.md b/README.md
index 3889af1e..d1bc38d9 100644
--- a/README.md
+++ b/README.md
@@ -20,7 +20,6 @@
 
 The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
 
-
 | Tool                                                                      |  Single-end reads  |  Version |
 | ------------------------------------------------------------------------- | :----------------: | :------: |
 | [Arriba](https://github.com/suhrig/arriba)                                |         :x:        |  `1.2.0` |

From d763cd3df21a2c3757dc432a5d863b54b7b6e992 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 11:35:11 +0100
Subject: [PATCH 226/359] prettier formatting

---
 .github/workflows/linting.yml                 |   1 +
 CHANGELOG.md                                  | 122 +++++++++---------
 README.md                                     |  24 ++--
 docs/usage.md                                 | 119 ++++++++---------
 modules/local/fusioncatcher/detect/meta.yml   |   2 +-
 modules/local/fusioncatcher/download/meta.yml |   2 +-
 modules/local/fusionreport/detect/meta.yml    |   2 +-
 modules/local/fusionreport/download/meta.yml  |   2 +-
 modules/local/kallisto/quant/meta.yml         |   2 +-
 modules/local/pizzly/detect/meta.yml          |   2 +-
 modules/local/squid/annotate/meta.yml         |   2 +-
 modules/local/squid/detect/meta.yml           |   3 +-
 modules/local/starfusion/detect/meta.yml      |   2 +-
 modules/local/starfusion/download/meta.yml    |   2 +-
 .../local/uscs/custom_gtftogenepred/meta.yml  |   2 +-
 nextflow_schema.json                          |   2 +-
 16 files changed, 146 insertions(+), 145 deletions(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 5991d7ab..e9cf5de3 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -78,4 +78,5 @@ jobs:
             lint_log.txt
             lint_results.md
             PR_number.txt
+
 #
diff --git a/CHANGELOG.md b/CHANGELOG.md
index a8e91fbe..20e69eaa 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -5,80 +5,80 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## v1.3.0dev nfcore/rnafusion - 2020/07/15
 
-* Using official STAR-Fusion container [#160](https://github.com/nf-core/rnafusion/issues/160)
+- Using official STAR-Fusion container [#160](https://github.com/nf-core/rnafusion/issues/160)
 
 ### Added
 
-* Added social preview image [#107](https://github.com/nf-core/rnafusion/issues/107)
-* Added support for GRCh37 genome assembly [#77](https://github.com/nf-core/rnafusion/issues/77)
+- Added social preview image [#107](https://github.com/nf-core/rnafusion/issues/107)
+- Added support for GRCh37 genome assembly [#77](https://github.com/nf-core/rnafusion/issues/77)
 
 ### Changed
 
-* Upgrade `fusion-report v2.1.2` to `fusion-report v2.1.3`
-* Upgrade `fusion-report v2.1.1` to `fusion-report v2.1.2`
-* Upgrade `fusion-report v2.1.0` to `fusion-report v2.1.1`
-* Upgrade `Arriba v1.1.0` to `Arriba v1.2.0`
-* Upgrade `fusion-report v2.0.2` to `fusion-report v2.1.0`
+- Upgrade `fusion-report v2.1.2` to `fusion-report v2.1.3`
+- Upgrade `fusion-report v2.1.1` to `fusion-report v2.1.2`
+- Upgrade `fusion-report v2.1.0` to `fusion-report v2.1.1`
+- Upgrade `Arriba v1.1.0` to `Arriba v1.2.0`
+- Upgrade `fusion-report v2.0.2` to `fusion-report v2.1.0`
 
 ### Fixed
 
-* Missing `strip-components` in `download-references.nf/star-fusion` [#148](https://github.com/nf-core/rnafusion/issues/148)
-* Missing version prefix for cdna [#143](https://github.com/nf-core/rnafusion/issues/143)
-* `samtools` missing header in empty file for FusionInspector [ref](https://github.com/STAR-Fusion/STAR-Fusion/issues/191)
-* Removed `profile` from helper scripts [#139](https://github.com/nf-core/rnafusion/issues/139)
-* Wrong url path for `Pfam-A.hmm.gz` [#140](https://github.com/nf-core/rnafusion/issues/140)
+- Missing `strip-components` in `download-references.nf/star-fusion` [#148](https://github.com/nf-core/rnafusion/issues/148)
+- Missing version prefix for cdna [#143](https://github.com/nf-core/rnafusion/issues/143)
+- `samtools` missing header in empty file for FusionInspector [ref](https://github.com/STAR-Fusion/STAR-Fusion/issues/191)
+- Removed `profile` from helper scripts [#139](https://github.com/nf-core/rnafusion/issues/139)
+- Wrong url path for `Pfam-A.hmm.gz` [#140](https://github.com/nf-core/rnafusion/issues/140)
 
 ### Removed
 
-* Removed `scripts/download-singularity-img.sh` and `download-singularity-img.nf` as they are not necessary any more
+- Removed `scripts/download-singularity-img.sh` and `download-singularity-img.nf` as they are not necessary any more
 
 ---
 
 ## [1.1.0] nfcore/rnafusion - 2020/02/10
 
-* Fusion gene detection tools:
-    * `Arriba v1.1.0`
-    * `Ericscript v0.5.5`
-    * `Fusioncatcher v1.20`
-    * `Pizzly v0.37.3`
-    * `Squid v1.5`
-    * `STAR-Fusion v1.6.0`
-* Visualization tools:
-    * `Arriba v1.1.0`
-    * `FusionInspector v1.3.1`
-* Other tools:
-    * `fusion-report v2.0.1`
-    * `FastQ v0.11.8`
-    * `MultiQC v1.7`
-    * `STAR aligner v2.7.0f`
+- Fusion gene detection tools:
+  - `Arriba v1.1.0`
+  - `Ericscript v0.5.5`
+  - `Fusioncatcher v1.20`
+  - `Pizzly v0.37.3`
+  - `Squid v1.5`
+  - `STAR-Fusion v1.6.0`
+- Visualization tools:
+  - `Arriba v1.1.0`
+  - `FusionInspector v1.3.1`
+- Other tools:
+  - `fusion-report v2.0.1`
+  - `FastQ v0.11.8`
+  - `MultiQC v1.7`
+  - `STAR aligner v2.7.0f`
 
 ### Added
 
-* Added `Arriba 1.1.0` [#63](https://github.com/nf-core/rnafusion/issues/63)
-* Added Batch mode [#54](https://github.com/nf-core/rnafusion/issues/54)
+- Added `Arriba 1.1.0` [#63](https://github.com/nf-core/rnafusion/issues/63)
+- Added Batch mode [#54](https://github.com/nf-core/rnafusion/issues/54)
 
 ### Changed
 
-* Updated examples and configurations
-* Upgraded `fusion-report v1.0.0` to `fusion-report v2.0.1`
-* Divided `running_tools` into fusion and visualization tools
-* Updated `STAR` in `Squid`, `Fusion-Inspector` version to `2.7.0f`
-* Upgraded `STAR-Fusion v1.5.0` to `STAR-Fusion v1.6.0` [#83](https://github.com/nf-core/rnafusion/issues/83)
-* Parameter `igenomesIgnore` renamed to `igenome` [#81](https://github.com/nf-core/rnafusion/issues/81)
-* Finished STAR-Fusion file renaming [#18](https://github.com/nf-core/rnafusion/issues/18)
-* Updated logos
-* Updated to nf-core `1.8` TEMPLATE
+- Updated examples and configurations
+- Upgraded `fusion-report v1.0.0` to `fusion-report v2.0.1`
+- Divided `running_tools` into fusion and visualization tools
+- Updated `STAR` in `Squid`, `Fusion-Inspector` version to `2.7.0f`
+- Upgraded `STAR-Fusion v1.5.0` to `STAR-Fusion v1.6.0` [#83](https://github.com/nf-core/rnafusion/issues/83)
+- Parameter `igenomesIgnore` renamed to `igenome` [#81](https://github.com/nf-core/rnafusion/issues/81)
+- Finished STAR-Fusion file renaming [#18](https://github.com/nf-core/rnafusion/issues/18)
+- Updated logos
+- Updated to nf-core `1.8` TEMPLATE
 
 ### Fixed
 
-* iGenomes optional, but not really [#91](https://github.com/nf-core/rnafusion/issues/91)
-* Updated `fusioncatcher` to latest `1.20` version also solving [#95](https://github.com/nf-core/rnafusion/issues/95)
+- iGenomes optional, but not really [#91](https://github.com/nf-core/rnafusion/issues/91)
+- Updated `fusioncatcher` to latest `1.20` version also solving [#95](https://github.com/nf-core/rnafusion/issues/95)
 
 ### Removed
 
-* Variables `pizzly_fasta` and `pizzly_gtf` have been removed and replaced with `transcript` and `gtf`
-* `Jenkisfile`, test configuration, pylintrc configuration
-* Removed `igenomes.config` because the pipeline only supports `Ensembl` version
+- Variables `pizzly_fasta` and `pizzly_gtf` have been removed and replaced with `transcript` and `gtf`
+- `Jenkisfile`, test configuration, pylintrc configuration
+- Removed `igenomes.config` because the pipeline only supports `Ensembl` version
 
 ---
 
@@ -86,13 +86,13 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Changed
 
-* Bumped nf-core template to 1.6 [#69](https://github.com/nf-core/rnafusion/pull/69)
+- Bumped nf-core template to 1.6 [#69](https://github.com/nf-core/rnafusion/pull/69)
 
 ### Fixed
 
-* Fixed COSMIC parameters not wrapped in quotes [#75](https://github.com/nf-core/rnafusion/issues/75)
-* Implemented output output for fusion tools [#72](https://github.com/nf-core/rnafusion/issues/72)
-* Fixed reference download link for STAR-Fusion [#71](https://github.com/nf-core/rnafusion/issues/71)
+- Fixed COSMIC parameters not wrapped in quotes [#75](https://github.com/nf-core/rnafusion/issues/75)
+- Implemented output output for fusion tools [#72](https://github.com/nf-core/rnafusion/issues/72)
+- Fixed reference download link for STAR-Fusion [#71](https://github.com/nf-core/rnafusion/issues/71)
 
 ---
 
@@ -100,27 +100,27 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Added
 
-* Added support for extra parameters for tools STAR-Fusion, FusionCatcher and fusion-report
-* Added example configuration for `singularity` and `docker`
-* Added [fusion-report](https://github.com/matq007/fusion-report) into the stack [#62](https://github.com/nf-core/rnafusion/issues/62), [#55](https://github.com/nf-core/rnafusion/issues/55), [#53](https://github.com/nf-core/rnafusion/issues/53), [#51](https://github.com/nf-core/rnafusion/issues/51)
-* Added nextflow helper script `download-singularity-img.nf`
-* Added nextflow helper script `download-references.nf`
-* Added `Jenkinsfile` for in-house testing
+- Added support for extra parameters for tools STAR-Fusion, FusionCatcher and fusion-report
+- Added example configuration for `singularity` and `docker`
+- Added [fusion-report](https://github.com/matq007/fusion-report) into the stack [#62](https://github.com/nf-core/rnafusion/issues/62), [#55](https://github.com/nf-core/rnafusion/issues/55), [#53](https://github.com/nf-core/rnafusion/issues/53), [#51](https://github.com/nf-core/rnafusion/issues/51)
+- Added nextflow helper script `download-singularity-img.nf`
+- Added nextflow helper script `download-references.nf`
+- Added `Jenkinsfile` for in-house testing
 
 ### Changed
 
-* Updated installation of `FusionCatcher` (available now on bioconda)
+- Updated installation of `FusionCatcher` (available now on bioconda)
 
 ### Fixed
 
-* Fixed empty symlinks (`input.X`) in fusion-report [#68](https://github.com/nf-core/rnafusion/issues/68)
-* Fixed FASTA issues [#60](https://github.com/nf-core/rnafusion/issues/60)
-* Fixed centralized nf-core/config [#64](https://github.com/nf-core/rnafusion/issues/64)
-* Fixed `scrape_software_versions.py` to parse tools versions correctly [#65](https://github.com/nf-core/rnafusion/issues/65)
+- Fixed empty symlinks (`input.X`) in fusion-report [#68](https://github.com/nf-core/rnafusion/issues/68)
+- Fixed FASTA issues [#60](https://github.com/nf-core/rnafusion/issues/60)
+- Fixed centralized nf-core/config [#64](https://github.com/nf-core/rnafusion/issues/64)
+- Fixed `scrape_software_versions.py` to parse tools versions correctly [#65](https://github.com/nf-core/rnafusion/issues/65)
 
 ### Removed
 
-* Removed `Singularity`
+- Removed `Singularity`
 
 ---
 
diff --git a/README.md b/README.md
index d1bc38d9..9ce31df5 100644
--- a/README.md
+++ b/README.md
@@ -20,16 +20,16 @@
 
 The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
 
-| Tool                                                                      |  Single-end reads  |  Version |
+| Tool                                                                      |  Single-end reads  | Version  |
 | ------------------------------------------------------------------------- | :----------------: | :------: |
-| [Arriba](https://github.com/suhrig/arriba)                                |         :x:        |  `1.2.0` |
-| [EricScript](https://sites.google.com/site/bioericscript/getting-started) |         :x:        |  `0.5.5` |
+| [Arriba](https://github.com/suhrig/arriba)                                |        :x:         | `1.2.0`  |
+| [EricScript](https://sites.google.com/site/bioericscript/getting-started) |        :x:         | `0.5.5`  |
 | [FusionCatcher](https://github.com/ndaniel/fusioncatcher)                 | :white_check_mark: |  `1.20`  |
-| [Fusion-Inspector](https://github.com/FusionInspector/FusionInspector)    |         :x:        |  `2.3.1` |
-| [fusion-report](https://github.com/matq007/fusion-report)                 |          -         |  `2.1.3` |
-| [Pizzly](https://github.com/pmelsted/pizzly)                              |         :x:        | `0.37.3` |
-| [Squid](https://github.com/Kingsford-Group/squid)                         |         :x:        |   `1.5`  |
-| [Star-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: |  `1.9.1` |
+| [Fusion-Inspector](https://github.com/FusionInspector/FusionInspector)    |        :x:         | `2.3.1`  |
+| [fusion-report](https://github.com/matq007/fusion-report)                 |         -          | `2.1.3`  |
+| [Pizzly](https://github.com/pmelsted/pizzly)                              |        :x:         | `0.37.3` |
+| [Squid](https://github.com/Kingsford-Group/squid)                         |        :x:         |  `1.5`   |
+| [Star-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: | `1.9.1`  |
 
 <!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
 
@@ -65,9 +65,9 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
 
-   ```console
-   nextflow run nf-core/rnafusion --input samplesheet.csv --outdir <OUTDIR> --genome GRCh38 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
-   ```
+```console
+nextflow run nf-core/rnafusion --input samplesheet.csv --outdir <OUTDIR> --genome GRCh38 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
+```
 
 ## Documentation
 
@@ -79,7 +79,7 @@ nf-core/rnafusion was originally written by Martin Proks, @praveenraj2018.
 
 We thank the following people for their extensive assistance in the development of this pipeline:
 
-* Annick Renevey (@rannick)
+- Annick Renevey (@rannick)
 
 ## Contributions and Support
 
diff --git a/docs/usage.md b/docs/usage.md
index ac8a37d6..74ad45bd 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -9,58 +9,58 @@
 - [:warning: Please read this documentation on the nf-core website: https://nf-co.re/rnafusion/usage](#warning-please-read-this-documentation-on-the-nf-core-website-httpsnf-corernafusionusage)
 - [Introduction](#introduction)
 - [Download references](#download-references)
-    - [Download all references](#download-all-references)
-    - [Download specific references](#download-specific-references)
-    - [Download and build CTAT](#download-and-build-ctat)
-    - [Download GRCh37 references](#download-grch37-references)
-    - [Tool reference requirements](#tool-reference-requirements)
+  - [Download all references](#download-all-references)
+  - [Download specific references](#download-specific-references)
+  - [Download and build CTAT](#download-and-build-ctat)
+  - [Download GRCh37 references](#download-grch37-references)
+  - [Tool reference requirements](#tool-reference-requirements)
 - [Running the pipeline](#running-the-pipeline)
-    - [Updating the pipeline](#updating-the-pipeline)
-    - [Reproducibility](#reproducibility)
+  - [Updating the pipeline](#updating-the-pipeline)
+  - [Reproducibility](#reproducibility)
 - [Core Nextflow arguments](#core-nextflow-arguments)
-    - [`-profile`](#-profile)
-    - [`-resume`](#-resume)
-    - [`-c`](#-c)
-        - [Custom resource requests](#custom-resource-requests)
-    - [Running in the background](#running-in-the-background)
-        - [Nextflow memory requirements](#nextflow-memory-requirements)
+  - [`-profile`](#-profile)
+  - [`-resume`](#-resume)
+  - [`-c`](#-c)
+    - [Custom resource requests](#custom-resource-requests)
+  - [Running in the background](#running-in-the-background)
+    - [Nextflow memory requirements](#nextflow-memory-requirements)
 - [Pipeline specific arguments](#pipeline-specific-arguments)
-    - [--input](#--input)
-    - [--single_end](#--single_end)
-    - [Tool flags](#tool-flags)
-    - [--arriba](#--arriba)
-    - [--ericscript](#--ericscript)
-    - [--fusioncatcher](#--fusioncatcher)
-    - [--fusion_report](#--fusion_report)
-    - [--pizzly](#--pizzly)
-    - [--squid](#--squid)
-    - [--star_fusion](#--star_fusion)
+  - [--input](#--input)
+  - [--single_end](#--single_end)
+  - [Tool flags](#tool-flags)
+  - [--arriba](#--arriba)
+  - [--ericscript](#--ericscript)
+  - [--fusioncatcher](#--fusioncatcher)
+  - [--fusion_report](#--fusion_report)
+  - [--pizzly](#--pizzly)
+  - [--squid](#--squid)
+  - [--star_fusion](#--star_fusion)
 - [Visualization flags](#visualization-flags)
-    - [--arriba_vis](#--arriba_vis)
-    - [--fusion_inspector](#--fusion_inspector)
+  - [--arriba_vis](#--arriba_vis)
+  - [--fusion_inspector](#--fusion_inspector)
 - [Reference genomes](#reference-genomes)
-    - [--arriba_ref](#--arriba_ref)
-    - [--databases](#--databases)
-    - [--ericscript_ref](#--ericscript_ref)
-    - [--fasta](#--fasta)
-    - [--fusioncatcher_ref](#--fusioncatcher_ref)
-    - [--genome](#--genome)
-    - [--gtf](#--gtf)
-    - [--star_index](#--star_index)
-    - [--star_fusion_ref](#--star_fusion_ref)
-    - [--transcript](#--transcript)
+  - [--arriba_ref](#--arriba_ref)
+  - [--databases](#--databases)
+  - [--ericscript_ref](#--ericscript_ref)
+  - [--fasta](#--fasta)
+  - [--fusioncatcher_ref](#--fusioncatcher_ref)
+  - [--genome](#--genome)
+  - [--gtf](#--gtf)
+  - [--star_index](#--star_index)
+  - [--star_fusion_ref](#--star_fusion_ref)
+  - [--transcript](#--transcript)
 - [Other command line parameters](#other-command-line-parameters)
-    - [--debug](#--debug)
-    - [--read_length](#--read_length)
-    - [--outdir](#--outdir)
-    - [--email](#--email)
-    - [--email_on_fail](#--email_on_fail)
-    - [--max_multiqc_email_size](#--max_multiqc_email_size)
-    - [-name](#-name)
-    - [--custom_config_version](#--custom_config_version)
-    - [--custom_config_base](#--custom_config_base)
+  - [--debug](#--debug)
+  - [--read_length](#--read_length)
+  - [--outdir](#--outdir)
+  - [--email](#--email)
+  - [--email_on_fail](#--email_on_fail)
+  - [--max_multiqc_email_size](#--max_multiqc_email_size)
+  - [-name](#-name)
+  - [--custom_config_version](#--custom_config_version)
+  - [--custom_config_base](#--custom_config_base)
 - [Job resources](#job-resources)
-    - [Automatic resubmission](#automatic-resubmission)
+  - [Automatic resubmission](#automatic-resubmission)
 
 ## Download references
 
@@ -136,16 +136,16 @@ nextflow run nf-core/rnafusion/build-ctat.nf \
 
 ### Tool reference requirements
 
-| Tool             |        FASTA       |         GTF        |     STAR-index     |     Genome     |       Other       |
+| Tool             |       FASTA        |        GTF         |     STAR-index     |     Genome     |       Other        |
 | ---------------- | :----------------: | :----------------: | :----------------: | :------------: | :----------------: |
 | Arriba           | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 | `custom_reference` |
-| EricScript       |         :x:        |         :x:        |         :x:        | GRCh37, GRCh38 | `custom_reference` |
-| FusionCatcher    |         :x:        |         :x:        |         :x:        |     GRCh38     | `custom_reference` |
-| Fusion-Inspector | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |  `ctat_genome_lib` |
-| fusion-report    |         :x:        |         :x:        |         :x:        | GRCh37, GRCh38 |     `databases`    |
-| Pizzly           |         :x:        | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |       `cDNA`       |
-| Squid            |         :x:        | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |          -         |
-| Star-Fusion      | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |  `ctat_genome_lib` |
+| EricScript       |        :x:         |        :x:         |        :x:         | GRCh37, GRCh38 | `custom_reference` |
+| FusionCatcher    |        :x:         |        :x:         |        :x:         |     GRCh38     | `custom_reference` |
+| Fusion-Inspector | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 | `ctat_genome_lib`  |
+| fusion-report    |        :x:         |        :x:         |        :x:         | GRCh37, GRCh38 |    `databases`     |
+| Pizzly           |        :x:         | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |       `cDNA`       |
+| Squid            |        :x:         | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 |         -          |
+| Star-Fusion      | :white_check_mark: | :white_check_mark: | :white_check_mark: | GRCh37, GRCh38 | `ctat_genome_lib`  |
 
 ## Samplesheet input
 
@@ -329,6 +329,7 @@ process {
 > If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
 
 <!-- TODO: adapt to current DSL2 parameters -->
+
 ### Tool-specific options
 
 For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes.
@@ -464,14 +465,14 @@ By default, the pipeline expects paired-end data. If you have single-end data, y
 If enabled, executes `Arriba` tool.
 
 - `--arriba_opt`
-    - Specify additional parameters. For more information, please refer to the [documentation](http://arriba.readthedocs.io/en/latest/quickstart/) of the tool.
+  - Specify additional parameters. For more information, please refer to the [documentation](http://arriba.readthedocs.io/en/latest/quickstart/) of the tool.
 
 ### --ericscript
 
 If enabled, executes `Ericscript` tool.
 
 - `--ericscript_opt`
-    - Specify additional parameters. For more information, please refer to the [documentation](https://sites.google.com/site/bioericscript/home) of the tool.
+  - Specify additional parameters. For more information, please refer to the [documentation](https://sites.google.com/site/bioericscript/home) of the tool.
 
 ### --fusioncatcher
 
@@ -480,21 +481,21 @@ If enabled, executes `Fusioncatcher` tool.
 > N.B. that Fusioncatcher is not available when using the `GRCh37` genome assembly.
 
 - `--fusioncatcher_opt`
-    - Specify additional parameters. For more information, please refer to the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md) of the tool.
+  - Specify additional parameters. For more information, please refer to the [documentation](https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md) of the tool.
 
 ### --fusion_report
 
 If enabled, download databases for `fusion-report`.
 
 - `fusion_report_opt`
-    - Specify additional parameters. For more information, please refer to the [documentation](https://matq007.github.io/fusion-report/#/) of the tool.
+  - Specify additional parameters. For more information, please refer to the [documentation](https://matq007.github.io/fusion-report/#/) of the tool.
 
 ### --pizzly
 
 If enabled, executes `Pizzly` tool.
 
 - `--pizzly_k`
-    - Number of k-mers. Default `31`.
+  - Number of k-mers. Default `31`.
 
 ### --squid
 
@@ -505,7 +506,7 @@ If enabled, executes `Squid` tool.
 If enabled, executes `STAR-Fusion` tool.
 
 - `--star_fusion_opt`
-    - Parameter for specifying additional parameters. For more information, please refer to the [documentation](https://github.com/STAR-Fusion/STAR-Fusion/wiki) of the tool.
+  - Parameter for specifying additional parameters. For more information, please refer to the [documentation](https://github.com/STAR-Fusion/STAR-Fusion/wiki) of the tool.
 
 ## Visualization flags
 
diff --git a/modules/local/fusioncatcher/detect/meta.yml b/modules/local/fusioncatcher/detect/meta.yml
index 795587fd..7c8ee425 100644
--- a/modules/local/fusioncatcher/detect/meta.yml
+++ b/modules/local/fusioncatcher/detect/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://github.com/ndaniel/fusioncatcher/wiki
       tool_dev_url: https://github.com/ndaniel/fusioncatcher
       doi: "10.1101/011650v1"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - meta:
diff --git a/modules/local/fusioncatcher/download/meta.yml b/modules/local/fusioncatcher/download/meta.yml
index dcc7eb9f..40421a4e 100644
--- a/modules/local/fusioncatcher/download/meta.yml
+++ b/modules/local/fusioncatcher/download/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
       tool_dev_url: https://github.com/ndaniel/fusioncatcher/
       doi: "10.1101/011650"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 output:
   - versions:
diff --git a/modules/local/fusionreport/detect/meta.yml b/modules/local/fusionreport/detect/meta.yml
index aeb23dc5..f3d5cd88 100644
--- a/modules/local/fusionreport/detect/meta.yml
+++ b/modules/local/fusionreport/detect/meta.yml
@@ -8,7 +8,7 @@ tools:
       homepage: https://github.com/matq007/fusion-report
       documentation: https://matq007.github.io/fusion-report/#/
       doi: "10.1101/011650"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - meta:
diff --git a/modules/local/fusionreport/download/meta.yml b/modules/local/fusionreport/download/meta.yml
index 3b297fd7..21a15a89 100644
--- a/modules/local/fusionreport/download/meta.yml
+++ b/modules/local/fusionreport/download/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
       tool_dev_url: https://github.com/ndaniel/fusioncatcher/
       doi: "10.1101/011650"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - username:
diff --git a/modules/local/kallisto/quant/meta.yml b/modules/local/kallisto/quant/meta.yml
index 836bf9ff..eef0a2b0 100644
--- a/modules/local/kallisto/quant/meta.yml
+++ b/modules/local/kallisto/quant/meta.yml
@@ -8,7 +8,7 @@ tools:
       documentation: https://pachterlab.github.io/kallisto/manual
       tool_dev_url: https://github.com/pachterlab/kallisto
       doi: ""
-      licence: ['BSD-2-Clause']
+      licence: ["BSD-2-Clause"]
 
 input:
   - fasta:
diff --git a/modules/local/pizzly/detect/meta.yml b/modules/local/pizzly/detect/meta.yml
index 97bf8f09..930b3a62 100644
--- a/modules/local/pizzly/detect/meta.yml
+++ b/modules/local/pizzly/detect/meta.yml
@@ -10,7 +10,7 @@ tools:
       documentation: https://github.com/pmelsted/pizzly
       tool_dev_url: https://github.com/pmelsted/pizzly
       doi: ""
-      licence: ['BSD-2-Clause']
+      licence: ["BSD-2-Clause"]
 
 input:
   - fasta:
diff --git a/modules/local/squid/annotate/meta.yml b/modules/local/squid/annotate/meta.yml
index ed5e6048..e1a1f0d2 100644
--- a/modules/local/squid/annotate/meta.yml
+++ b/modules/local/squid/annotate/meta.yml
@@ -10,7 +10,7 @@ tools:
       documentation: https://github.com/Kingsford-Group/squid
       tool_dev_url: https://github.com/Kingsford-Group/squid
       doi: ""
-      licence: ['BSD-3-Clause']
+      licence: ["BSD-3-Clause"]
 
 input:
   - fusions:
diff --git a/modules/local/squid/detect/meta.yml b/modules/local/squid/detect/meta.yml
index db1cc2bb..a7f1e61a 100644
--- a/modules/local/squid/detect/meta.yml
+++ b/modules/local/squid/detect/meta.yml
@@ -10,10 +10,9 @@ tools:
       documentation: https://github.com/Kingsford-Group/squid
       tool_dev_url: https://github.com/Kingsford-Group/squid
       doi: ""
-      licence: ['BSD-3-Clause']
+      licence: ["BSD-3-Clause"]
 
 input:
-
   - meta:
       type: map
       description: |
diff --git a/modules/local/starfusion/detect/meta.yml b/modules/local/starfusion/detect/meta.yml
index 098c6596..136facba 100644
--- a/modules/local/starfusion/detect/meta.yml
+++ b/modules/local/starfusion/detect/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki
       tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion/releases
       doi: "10.1101/120295v1"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - meta:
diff --git a/modules/local/starfusion/download/meta.yml b/modules/local/starfusion/download/meta.yml
index 37f71f06..dc7fee75 100644
--- a/modules/local/starfusion/download/meta.yml
+++ b/modules/local/starfusion/download/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki/installing-star-fusion
       tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion
       doi: "10.1186/s13059-019-1842-9"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - genome_version:
diff --git a/modules/local/uscs/custom_gtftogenepred/meta.yml b/modules/local/uscs/custom_gtftogenepred/meta.yml
index 896e2ef6..09711f43 100644
--- a/modules/local/uscs/custom_gtftogenepred/meta.yml
+++ b/modules/local/uscs/custom_gtftogenepred/meta.yml
@@ -8,7 +8,7 @@ tools:
       documentation: https://pachterlab.github.io/kallisto/manual
       tool_dev_url: https://github.com/pachterlab/kallisto
       doi: ""
-      licence: ['BSD-2-Clause']
+      licence: ["BSD-2-Clause"]
 
 input:
   - fasta:
diff --git a/nextflow_schema.json b/nextflow_schema.json
index e5226277..d626720d 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -445,4 +445,4 @@
             "$ref": "#/definitions/generic_options"
         }
     ]
-}
\ No newline at end of file
+}

From 44fd4c8e9db5610ad979ea6ab89071cf42d32869 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 11:48:36 +0100
Subject: [PATCH 227/359] nf-core lint

---
 modules.json | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules.json b/modules.json
index c52cced8..dddcb9d5 100644
--- a/modules.json
+++ b/modules.json
@@ -10,7 +10,7 @@
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "custom/dumpsoftwareversions": {
-                "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
+                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "fastqc": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
@@ -53,4 +53,4 @@
             }
         }
     }
-}
+}
\ No newline at end of file

From 6decde961382cbadc0ea15be34a48ad677ca7556 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 11:50:15 +0100
Subject: [PATCH 228/359] nf-core lint/prettier

---
 modules.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules.json b/modules.json
index dddcb9d5..c1cbe4ab 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}

From de5a9f3e9ba0496050af73973970873fdd52a9f7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 13:19:24 +0100
Subject: [PATCH 229/359] CHANGELOG.md

---
 CHANGELOG.md | 151 +++++++++++++++++++++++++++++++++++++++++++++++++++
 1 file changed, 151 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index a8e91fbe..18cda614 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,6 +3,157 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## [2.0.0] nfcore/rnafusion - 2022/03/??
+
+Update to DSL2 and newer software/reference versions
+
+### Non-DSL2 related changes
+- replace gtf file with chr.gtf: Please confirm if this change is ok for the whole pipeline
+- remove subdirectory with params.genome as only GRCh38 is supported (not necessary but helped me see the output more clearly, can be reversed)
+- modification of output parameters to make sure every important file is published
+- additional build_references flag to trigger the workflow to build references
+- container for starfusion is changed and the absolute path to prep_genome_lib.pl is given
+    --fasta argument is not required when executing the workflow to build references
+- increase cpu + memory for process STARFUSION_DOWNLOAD
+- ci test done on stubs of reference building subprocesses ensembl and arriba
+- **For the moment, the rnafusion workflow (to analyse data and not build the references) is removed as dependencies and links would have to be fixed in the rnafusion workflow for execution of the build references otherwise**
+
+---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
+
+### Added
+
+* Added `qualimap/rnaseq v2.2.2d` from nf-core modules
+* Added UCSC `gtfToGenePred v377`
+* Added `picard CollectRnaSeqMetrics v2.26.10`
+* Added `picard MarkDuplicates v2.26.10` from nf-core modules
+* Added `cat/fastqc` from nf-core modules
+* Added possibility for manually feeding the results of fusions from different tools to speed-up reruns
+
+### Changed
+
+* Upgrade to `nf-core/tools v2.3`
+* Upgrade `Arriba v1.2.0` to `Arriba v2.1.0`
+* Upgrade `FusionCatcher v1.20` to `FusionCatcher v1.33`
+* Upgrade `STAR-fusion v1.8.1` to `STAR-fusion v1.10.1`
+* Upgrade `STAR v2.7.1` to `STAR v2.7.9`
+* Upgrade `fusion-report v2.1.3` to `fusion-report v2.1.5`
+* Upgrade `kallisto v0.44.0` to `kallisto v0.46.2`
+* Upgrade `fastqc v0.11.8` to `fastqc v0.11.9`
+* Upgrade `samtools v1.9` to `samtools v1.15`
+* Upgrade `arriba` references from `v1.2.0` to `v2.1.0`
+* Upgrade `fusioncatcher` references from `v98` to `v102`
+* Use `arriba` (detect only), `kallisto` and `STAR` from nf-core modules
+
+Parameters for `STAR` for `arriba` changed from:
+
+```bash
+--readFilesCommand zcat \\
+        --outSAMtype BAM Unsorted \\
+--outStd BAM_Unsorted \\
+--outSAMunmapped Within \\
+--outBAMcompression 0 \\
+--outFilterMultimapNmax 1 \\
+--outFilterMismatchNmax 3 \\
+--chimSegmentMin 10 \\
+--chimOutType WithinBAM SoftClip \\
+--chimJunctionOverhangMin 10 \\
+--chimScoreMin 1 \\
+--chimScoreDropMax 30 \\
+--chimScoreJunctionNonGTAG 0 \\
+--chimScoreSeparation 1 \\
+--alignSJstitchMismatchNmax 5 -1 5 5 \\
+--chimSegmentReadGapMax 3 \\
+--sjdbOverhang ${params.read_length - 1}
+```
+
+to
+
+```bash
+--readFilesCommand zcat \
+--outSAMtype BAM Unsorted \
+--outSAMunmapped Within \
+--outBAMcompression 0 \
+--outFilterMultimapNmax 50 \
+--peOverlapNbasesMin 10 \
+--alignSplicedMateMapLminOverLmate 0.5 \
+--alignSJstitchMismatchNmax 5 -1 5 5 \
+--chimSegmentMin 10 \
+--chimOutType WithinBAM HardClip \
+--chimJunctionOverhangMin 10 \
+--chimScoreDropMax 30 \
+--chimScoreJunctionNonGTAG 0 \
+--chimScoreSeparation 1 \
+--chimSegmentReadGapMax 3 \
+--chimMultimapNmax 50
+```
+
+As recommended [here](https://arriba.readthedocs.io/en/latest/workflow/).
+
+Parameters for `STAR` for `STAR-fusion` changed from:
+
+```bash
+--twopassMode Basic \\
+--outReadsUnmapped None \\
+--chimSegmentMin 12 \\
+--chimJunctionOverhangMin 12 \\
+--alignSJDBoverhangMin 10 \\
+--alignMatesGapMax 100000 \\
+--alignIntronMax 100000 \\
+--chimSegmentReadGapMax 3 \\
+--alignSJstitchMismatchNmax 5 -1 5 5 \\
+--runThreadN ${task.cpus} \\
+--outSAMstrandField intronMotif ${avail_mem} \\
+--outSAMunmapped Within \\
+--outSAMtype BAM Unsorted \\
+--outSAMattrRGline ID:GRPundef \\
+--chimMultimapScoreRange 10 \\
+--chimMultimapNmax 10 \\
+--chimNonchimScoreDropMin 10 \\
+--peOverlapNbasesMin 12 \\
+--peOverlapMMp 0.1 \\
+--readFilesCommand zcat \\
+--sjdbOverhang ${params.read_length - 1} \\
+--chimOutJunctionFormat 1
+```
+
+to
+
+```bash
+--outReadsUnmapped None \
+--readFilesCommand zcat \
+--outSAMtype BAM SortedByCoordinate \
+--outSAMstrandField intronMotif \
+--outSAMunmapped Within \
+--chimSegmentMin 12 \
+--chimJunctionOverhangMin 8 \
+--chimOutJunctionFormat 1 \
+--alignSJDBoverhangMin 10 \
+--alignMatesGapMax 100000 \
+--alignIntronMax 100000 \
+--alignSJstitchMismatchNmax 5 -1 5 5 \
+--chimMultimapScoreRange 3 \
+--chimScoreJunctionNonGTAG -4 \
+--chimMultimapNmax 20 \
+--chimNonchimScoreDropMin 10 \
+--peOverlapNbasesMin 12 \
+--peOverlapMMp 0.1 \
+--alignInsertionFlush Right \
+--alignSplicedMateMapLminOverLmate 0 \
+--alignSplicedMateMapLmin 30 \
+--chimOutType Junctions
+```
+
+`Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz` used for squid and arriba, `Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz` used for STAR-fusion and the quality control as the quality control is based on the STAR-fusion alignment.
+
+### Fixed
+
+
+### Removed
+
+* Ericscript tool
+* GRCh37 support
+* Running with conda
+
 ## v1.3.0dev nfcore/rnafusion - 2020/07/15
 
 * Using official STAR-Fusion container [#160](https://github.com/nf-core/rnafusion/issues/160)

From 4470d0bca3ba7eec624985d3c8f181a22a9b7f95 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 13:39:37 +0100
Subject: [PATCH 230/359] update citations

---
 CITATIONS.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CITATIONS.md b/CITATIONS.md
index 07feb112..043c42b7 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -41,7 +41,7 @@
     > Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner Bioinformatics. 2013 Jan 1;29(1):15-21. doi: 10.1093/bioinformatics/bts635. Epub 2012 Oct 25. PubMed PMID: 23104886; PubMed Central PMCID: PMC3530905.
 
 * [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)
-    > Haas BJ, Dobin A, Stransky N, Li B, Yang X, Tickle T, Bankapur A, Ganote C, Doak TG, Pochet N, Sun J, Wu CJ, Gingeras TR, Regev A. STAR-Fusion: Fast and Accurate Fusion Transcript Detection from RNA-Seq. BioRxiv 2017 Mar. doi: 10.1101/120295.
+    > Haas BJ, Dobin A, Li B, Stransky N, Pochet N, Regev A. Accuracy assessment of fusion transcript detection via read-mapping and de novo fusion transcript assembly-based methods. Genome Biology 2019 Oct;20,213. doi: 10.1186/s13059-019-1842-9
 
 ## Software packaging/containerisation tools
 

From 872eac966f7257ac7408c8dff78cabd041f819bf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 13:44:26 +0100
Subject: [PATCH 231/359] prettier

---
 .github/CONTRIBUTING.md                       |  12 +-
 .github/ISSUE_TEMPLATE/bug_report.yml         |   2 -
 .github/PULL_REQUEST_TEMPLATE.md              |  20 +-
 .github/workflows/awsfulltest.yml             |   2 +-
 .github/workflows/awstest.yml                 |   4 +-
 .github/workflows/branch.yml                  |   2 -
 .github/workflows/ci.yml                      |   8 +-
 .github/workflows/linting.yml                 |   6 +-
 .github/workflows/linting_comment.yml         |   2 -
 .markdownlint.yml                             |  16 +-
 .nf-core.yml                                  |   2 +-
 CHANGELOG.md                                  | 184 ++++++-------
 CITATIONS.md                                  |  45 ++--
 CODE_OF_CONDUCT.md                            |  80 +++---
 README.md                                     |  69 ++---
 assets/email_template.html                    | 167 ++++++++----
 assets/multiqc_config.yaml                    |  14 +-
 assets/schema_input.json                      |   5 +-
 docs/README.md                                |   8 +-
 docs/output.md                                | 241 +++++++++---------
 docs/usage.md                                 |  62 ++---
 modules.json                                  |   2 +-
 modules/local/fusioncatcher/detect/meta.yml   |   8 +-
 modules/local/fusioncatcher/download/meta.yml |   8 +-
 modules/local/fusioninspector/meta.yml        |   6 +-
 modules/local/fusionreport/detect/meta.yml    |  14 +-
 modules/local/fusionreport/download/meta.yml  |   8 +-
 modules/local/kallisto/quant/meta.yml         |   2 +-
 modules/local/pizzly/detect/meta.yml          |   2 +-
 modules/local/squid/annotate/meta.yml         |   2 +-
 modules/local/squid/detect/meta.yml           |   3 +-
 modules/local/starfusion/detect/meta.yml      |   2 +-
 modules/local/starfusion/download/meta.yml    |   2 +-
 .../custom/dumpsoftwareversions/meta.yml      |   2 +-
 nextflow_schema.json                          |   6 +-
 35 files changed, 552 insertions(+), 466 deletions(-)

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 4dfbe44c..9807f30c 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -16,7 +16,7 @@ Contributions to the code are even more welcome ;)
 If you'd like to write some code for nf-core/rnafusion, the standard workflow is as follows:
 
 1. Check that there isn't already an issue about your idea in the [nf-core/rnafusion issues](https://github.com/nf-core/rnafusion/issues) to avoid duplicating work
-    * If there isn't one already, please create one so that others know you're working on this
+    - If there isn't one already, please create one so that others know you're working on this
 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rnafusion repository](https://github.com/nf-core/rnafusion) to your GitHub account
 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
 4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
@@ -49,9 +49,9 @@ These tests are run both with the latest available version of `Nextflow` and als
 
 :warning: Only in the unlikely and regretful event of a release happening with a bug.
 
-* On your own fork, make a new branch `patch` based on `upstream/master`.
-* Fix the bug, and bump version (X.Y.Z+1).
-* A PR should be made on `master` from patch to directly this particular bug.
+-   On your own fork, make a new branch `patch` based on `upstream/master`.
+-   Fix the bug, and bump version (X.Y.Z+1).
+-   A PR should be made on `master` from patch to directly this particular bug.
 
 ## Getting help
 
@@ -92,8 +92,8 @@ The process resources can be passed on to the tool dynamically within the proces
 
 Please use the following naming schemes, to make it easy to understand what is going where.
 
-* initial process channel: `ch_output_from_<process>`
-* intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
+-   initial process channel: `ch_output_from_<process>`
+-   intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
 
 ### Nextflow version bumping
 
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index a3de1c24..a172d421 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -1,9 +1,7 @@
-
 name: Bug report
 description: Report something that is broken or incorrect
 labels: bug
 body:
-
   - type: markdown
     attributes:
       value: |
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 026428de..bca6064b 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -14,13 +14,13 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
 
 ## PR checklist
 
-- [ ] This comment contains a description of changes (with reason).
-- [ ] If you've fixed a bug or added code that should be tested, add tests!
-    - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
-    - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
-- [ ] Make sure your code lints (`nf-core lint`).
-- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
-- [ ] Usage Documentation in `docs/usage.md` is updated.
-- [ ] Output Documentation in `docs/output.md` is updated.
-- [ ] `CHANGELOG.md` is updated.
-- [ ] `README.md` is updated (including new tool citations and authors/contributors).
+-   [ ] This comment contains a description of changes (with reason).
+-   [ ] If you've fixed a bug or added code that should be tested, add tests!
+    -   [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+    -   [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
+-   [ ] Make sure your code lints (`nf-core lint`).
+-   [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
+-   [ ] Usage Documentation in `docs/usage.md` is updated.
+-   [ ] Output Documentation in `docs/output.md` is updated.
+-   [ ] `CHANGELOG.md` is updated.
+-   [ ] `README.md` is updated (including new tool citations and authors/contributors).
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 8dfc3a70..a9429819 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -31,4 +31,4 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
             }
           profiles: test_full,aws_tower
-          pre_run_script: 'export NXF_VER=21.10.3'
+          pre_run_script: "export NXF_VER=21.10.3"
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 2c933fcc..015384e3 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -12,7 +12,7 @@ jobs:
     steps:
       - name: Launch workflow via tower
         uses: nf-core/tower-action@v2
-        
+
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
@@ -25,4 +25,4 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-test-${{ github.sha }}"
             }
           profiles: test,aws_tower
-          pre_run_script: 'export NXF_VER=21.10.3'
+          pre_run_script: "export NXF_VER=21.10.3"
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 37b7dea8..ccb7211b 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -15,7 +15,6 @@ jobs:
         run: |
           { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
 
-
       # If the above check failed, post a comment on the PR explaining the failure
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
       - name: Post PR comment
@@ -43,4 +42,3 @@ jobs:
             Thanks again for your contribution!
           repo-token: ${{ secrets.GITHUB_TOKEN }}
           allow-repeats: false
-
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 37e371f5..901d47a3 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -23,11 +23,11 @@ jobs:
         # Nextflow versions
         include:
           # Test pipeline minimum Nextflow version
-          - NXF_VER: '21.10.3'
-            NXF_EDGE: ''
+          - NXF_VER: "21.10.3"
+            NXF_EDGE: ""
           # Test latest edge release of Nextflow
-          - NXF_VER: ''
-            NXF_EDGE: '1'
+          - NXF_VER: ""
+            NXF_EDGE: "1"
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v2
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index faa54e71..8728656d 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -95,7 +95,6 @@ jobs:
   nf-core:
     runs-on: ubuntu-latest
     steps:
-
       - name: Check out pipeline code
         uses: actions/checkout@v2
 
@@ -108,8 +107,8 @@ jobs:
 
       - uses: actions/setup-python@v1
         with:
-          python-version: '3.6'
-          architecture: 'x64'
+          python-version: "3.6"
+          architecture: "x64"
 
       - name: Install dependencies
         run: |
@@ -136,4 +135,3 @@ jobs:
             lint_log.txt
             lint_results.md
             PR_number.txt
-
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 44d72994..04758f61 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -1,4 +1,3 @@
-
 name: nf-core linting comment
 # This workflow is triggered after the linting action is complete
 # It posts an automated comment to the PR, even if the PR is coming from a fork
@@ -27,4 +26,3 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
           path: linting-logs/lint_results.md
-
diff --git a/.markdownlint.yml b/.markdownlint.yml
index 9e605fcf..779ba6c0 100644
--- a/.markdownlint.yml
+++ b/.markdownlint.yml
@@ -2,13 +2,13 @@
 default: true
 line-length: false
 ul-indent:
-    indent: 4
+  indent: 4
 no-duplicate-header:
-    siblings_only: true
+  siblings_only: true
 no-inline-html:
-    allowed_elements:
-        - img
-        - p
-        - kbd
-        - details
-        - summary
+  allowed_elements:
+    - img
+    - p
+    - kbd
+    - details
+    - summary
diff --git a/.nf-core.yml b/.nf-core.yml
index b1370fd9..c212cb4a 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,4 +1,4 @@
 lint:
-    files_unchanged:
+  files_unchanged:
     # Added to ignore linting error: actions/setup-node@v1 until fixed release
     - .github/workflows/linting.yml
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 18cda614..065acda1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -8,41 +8,42 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 Update to DSL2 and newer software/reference versions
 
 ### Non-DSL2 related changes
-- replace gtf file with chr.gtf: Please confirm if this change is ok for the whole pipeline
-- remove subdirectory with params.genome as only GRCh38 is supported (not necessary but helped me see the output more clearly, can be reversed)
-- modification of output parameters to make sure every important file is published
-- additional build_references flag to trigger the workflow to build references
-- container for starfusion is changed and the absolute path to prep_genome_lib.pl is given
+
+-   replace gtf file with chr.gtf: Please confirm if this change is ok for the whole pipeline
+-   remove subdirectory with params.genome as only GRCh38 is supported (not necessary but helped me see the output more clearly, can be reversed)
+-   modification of output parameters to make sure every important file is published
+-   additional build_references flag to trigger the workflow to build references
+-   container for starfusion is changed and the absolute path to prep_genome_lib.pl is given
     --fasta argument is not required when executing the workflow to build references
-- increase cpu + memory for process STARFUSION_DOWNLOAD
-- ci test done on stubs of reference building subprocesses ensembl and arriba
-- **For the moment, the rnafusion workflow (to analyse data and not build the references) is removed as dependencies and links would have to be fixed in the rnafusion workflow for execution of the build references otherwise**
+-   increase cpu + memory for process STARFUSION_DOWNLOAD
+-   ci test done on stubs of reference building subprocesses ensembl and arriba
+-   **For the moment, the rnafusion workflow (to analyse data and not build the references) is removed as dependencies and links would have to be fixed in the rnafusion workflow for execution of the build references otherwise**
 
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
+---
 
 ### Added
 
-* Added `qualimap/rnaseq v2.2.2d` from nf-core modules
-* Added UCSC `gtfToGenePred v377`
-* Added `picard CollectRnaSeqMetrics v2.26.10`
-* Added `picard MarkDuplicates v2.26.10` from nf-core modules
-* Added `cat/fastqc` from nf-core modules
-* Added possibility for manually feeding the results of fusions from different tools to speed-up reruns
+-   Added `qualimap/rnaseq v2.2.2d` from nf-core modules
+-   Added UCSC `gtfToGenePred v377`
+-   Added `picard CollectRnaSeqMetrics v2.26.10`
+-   Added `picard MarkDuplicates v2.26.10` from nf-core modules
+-   Added `cat/fastqc` from nf-core modules
+-   Added possibility for manually feeding the results of fusions from different tools to speed-up reruns
 
 ### Changed
 
-* Upgrade to `nf-core/tools v2.3`
-* Upgrade `Arriba v1.2.0` to `Arriba v2.1.0`
-* Upgrade `FusionCatcher v1.20` to `FusionCatcher v1.33`
-* Upgrade `STAR-fusion v1.8.1` to `STAR-fusion v1.10.1`
-* Upgrade `STAR v2.7.1` to `STAR v2.7.9`
-* Upgrade `fusion-report v2.1.3` to `fusion-report v2.1.5`
-* Upgrade `kallisto v0.44.0` to `kallisto v0.46.2`
-* Upgrade `fastqc v0.11.8` to `fastqc v0.11.9`
-* Upgrade `samtools v1.9` to `samtools v1.15`
-* Upgrade `arriba` references from `v1.2.0` to `v2.1.0`
-* Upgrade `fusioncatcher` references from `v98` to `v102`
-* Use `arriba` (detect only), `kallisto` and `STAR` from nf-core modules
+-   Upgrade to `nf-core/tools v2.3`
+-   Upgrade `Arriba v1.2.0` to `Arriba v2.1.0`
+-   Upgrade `FusionCatcher v1.20` to `FusionCatcher v1.33`
+-   Upgrade `STAR-fusion v1.8.1` to `STAR-fusion v1.10.1`
+-   Upgrade `STAR v2.7.1` to `STAR v2.7.9`
+-   Upgrade `fusion-report v2.1.3` to `fusion-report v2.1.5`
+-   Upgrade `kallisto v0.44.0` to `kallisto v0.46.2`
+-   Upgrade `fastqc v0.11.8` to `fastqc v0.11.9`
+-   Upgrade `samtools v1.9` to `samtools v1.15`
+-   Upgrade `arriba` references from `v1.2.0` to `v2.1.0`
+-   Upgrade `fusioncatcher` references from `v98` to `v102`
+-   Use `arriba` (detect only), `kallisto` and `STAR` from nf-core modules
 
 Parameters for `STAR` for `arriba` changed from:
 
@@ -147,89 +148,88 @@ to
 
 ### Fixed
 
-
 ### Removed
 
-* Ericscript tool
-* GRCh37 support
-* Running with conda
+-   Ericscript tool
+-   GRCh37 support
+-   Running with conda
 
 ## v1.3.0dev nfcore/rnafusion - 2020/07/15
 
-* Using official STAR-Fusion container [#160](https://github.com/nf-core/rnafusion/issues/160)
+-   Using official STAR-Fusion container [#160](https://github.com/nf-core/rnafusion/issues/160)
 
 ### Added
 
-* Added social preview image [#107](https://github.com/nf-core/rnafusion/issues/107)
-* Added support for GRCh37 genome assembly [#77](https://github.com/nf-core/rnafusion/issues/77)
+-   Added social preview image [#107](https://github.com/nf-core/rnafusion/issues/107)
+-   Added support for GRCh37 genome assembly [#77](https://github.com/nf-core/rnafusion/issues/77)
 
 ### Changed
 
-* Upgrade `fusion-report v2.1.2` to `fusion-report v2.1.3`
-* Upgrade `fusion-report v2.1.1` to `fusion-report v2.1.2`
-* Upgrade `fusion-report v2.1.0` to `fusion-report v2.1.1`
-* Upgrade `Arriba v1.1.0` to `Arriba v1.2.0`
-* Upgrade `fusion-report v2.0.2` to `fusion-report v2.1.0`
+-   Upgrade `fusion-report v2.1.2` to `fusion-report v2.1.3`
+-   Upgrade `fusion-report v2.1.1` to `fusion-report v2.1.2`
+-   Upgrade `fusion-report v2.1.0` to `fusion-report v2.1.1`
+-   Upgrade `Arriba v1.1.0` to `Arriba v1.2.0`
+-   Upgrade `fusion-report v2.0.2` to `fusion-report v2.1.0`
 
 ### Fixed
 
-* Missing `strip-components` in `download-references.nf/star-fusion` [#148](https://github.com/nf-core/rnafusion/issues/148)
-* Missing version prefix for cdna [#143](https://github.com/nf-core/rnafusion/issues/143)
-* `samtools` missing header in empty file for FusionInspector [ref](https://github.com/STAR-Fusion/STAR-Fusion/issues/191)
-* Removed `profile` from helper scripts [#139](https://github.com/nf-core/rnafusion/issues/139)
-* Wrong url path for `Pfam-A.hmm.gz` [#140](https://github.com/nf-core/rnafusion/issues/140)
+-   Missing `strip-components` in `download-references.nf/star-fusion` [#148](https://github.com/nf-core/rnafusion/issues/148)
+-   Missing version prefix for cdna [#143](https://github.com/nf-core/rnafusion/issues/143)
+-   `samtools` missing header in empty file for FusionInspector [ref](https://github.com/STAR-Fusion/STAR-Fusion/issues/191)
+-   Removed `profile` from helper scripts [#139](https://github.com/nf-core/rnafusion/issues/139)
+-   Wrong url path for `Pfam-A.hmm.gz` [#140](https://github.com/nf-core/rnafusion/issues/140)
 
 ### Removed
 
-* Removed `scripts/download-singularity-img.sh` and `download-singularity-img.nf` as they are not necessary any more
+-   Removed `scripts/download-singularity-img.sh` and `download-singularity-img.nf` as they are not necessary any more
 
 ---
 
 ## [1.1.0] nfcore/rnafusion - 2020/02/10
 
-* Fusion gene detection tools:
-    * `Arriba v1.1.0`
-    * `Ericscript v0.5.5`
-    * `Fusioncatcher v1.20`
-    * `Pizzly v0.37.3`
-    * `Squid v1.5`
-    * `STAR-Fusion v1.6.0`
-* Visualization tools:
-    * `Arriba v1.1.0`
-    * `FusionInspector v1.3.1`
-* Other tools:
-    * `fusion-report v2.0.1`
-    * `FastQ v0.11.8`
-    * `MultiQC v1.7`
-    * `STAR aligner v2.7.0f`
+-   Fusion gene detection tools:
+    -   `Arriba v1.1.0`
+    -   `Ericscript v0.5.5`
+    -   `Fusioncatcher v1.20`
+    -   `Pizzly v0.37.3`
+    -   `Squid v1.5`
+    -   `STAR-Fusion v1.6.0`
+-   Visualization tools:
+    -   `Arriba v1.1.0`
+    -   `FusionInspector v1.3.1`
+-   Other tools:
+    -   `fusion-report v2.0.1`
+    -   `FastQ v0.11.8`
+    -   `MultiQC v1.7`
+    -   `STAR aligner v2.7.0f`
 
 ### Added
 
-* Added `Arriba 1.1.0` [#63](https://github.com/nf-core/rnafusion/issues/63)
-* Added Batch mode [#54](https://github.com/nf-core/rnafusion/issues/54)
+-   Added `Arriba 1.1.0` [#63](https://github.com/nf-core/rnafusion/issues/63)
+-   Added Batch mode [#54](https://github.com/nf-core/rnafusion/issues/54)
 
 ### Changed
 
-* Updated examples and configurations
-* Upgraded `fusion-report v1.0.0` to `fusion-report v2.0.1`
-* Divided `running_tools` into fusion and visualization tools
-* Updated `STAR` in `Squid`, `Fusion-Inspector` version to `2.7.0f`
-* Upgraded `STAR-Fusion v1.5.0` to `STAR-Fusion v1.6.0` [#83](https://github.com/nf-core/rnafusion/issues/83)
-* Parameter `igenomesIgnore` renamed to `igenome` [#81](https://github.com/nf-core/rnafusion/issues/81)
-* Finished STAR-Fusion file renaming [#18](https://github.com/nf-core/rnafusion/issues/18)
-* Updated logos
-* Updated to nf-core `1.8` TEMPLATE
+-   Updated examples and configurations
+-   Upgraded `fusion-report v1.0.0` to `fusion-report v2.0.1`
+-   Divided `running_tools` into fusion and visualization tools
+-   Updated `STAR` in `Squid`, `Fusion-Inspector` version to `2.7.0f`
+-   Upgraded `STAR-Fusion v1.5.0` to `STAR-Fusion v1.6.0` [#83](https://github.com/nf-core/rnafusion/issues/83)
+-   Parameter `igenomesIgnore` renamed to `igenome` [#81](https://github.com/nf-core/rnafusion/issues/81)
+-   Finished STAR-Fusion file renaming [#18](https://github.com/nf-core/rnafusion/issues/18)
+-   Updated logos
+-   Updated to nf-core `1.8` TEMPLATE
 
 ### Fixed
 
-* iGenomes optional, but not really [#91](https://github.com/nf-core/rnafusion/issues/91)
-* Updated `fusioncatcher` to latest `1.20` version also solving [#95](https://github.com/nf-core/rnafusion/issues/95)
+-   iGenomes optional, but not really [#91](https://github.com/nf-core/rnafusion/issues/91)
+-   Updated `fusioncatcher` to latest `1.20` version also solving [#95](https://github.com/nf-core/rnafusion/issues/95)
 
 ### Removed
 
-* Variables `pizzly_fasta` and `pizzly_gtf` have been removed and replaced with `transcript` and `gtf`
-* `Jenkisfile`, test configuration, pylintrc configuration
-* Removed `igenomes.config` because the pipeline only supports `Ensembl` version
+-   Variables `pizzly_fasta` and `pizzly_gtf` have been removed and replaced with `transcript` and `gtf`
+-   `Jenkisfile`, test configuration, pylintrc configuration
+-   Removed `igenomes.config` because the pipeline only supports `Ensembl` version
 
 ---
 
@@ -237,13 +237,13 @@ to
 
 ### Changed
 
-* Bumped nf-core template to 1.6 [#69](https://github.com/nf-core/rnafusion/pull/69)
+-   Bumped nf-core template to 1.6 [#69](https://github.com/nf-core/rnafusion/pull/69)
 
 ### Fixed
 
-* Fixed COSMIC parameters not wrapped in quotes [#75](https://github.com/nf-core/rnafusion/issues/75)
-* Implemented output output for fusion tools [#72](https://github.com/nf-core/rnafusion/issues/72)
-* Fixed reference download link for STAR-Fusion [#71](https://github.com/nf-core/rnafusion/issues/71)
+-   Fixed COSMIC parameters not wrapped in quotes [#75](https://github.com/nf-core/rnafusion/issues/75)
+-   Implemented output output for fusion tools [#72](https://github.com/nf-core/rnafusion/issues/72)
+-   Fixed reference download link for STAR-Fusion [#71](https://github.com/nf-core/rnafusion/issues/71)
 
 ---
 
@@ -251,27 +251,27 @@ to
 
 ### Added
 
-* Added support for extra parameters for tools STAR-Fusion, FusionCatcher and fusion-report
-* Added example configuration for `singularity` and `docker`
-* Added [fusion-report](https://github.com/matq007/fusion-report) into the stack [#62](https://github.com/nf-core/rnafusion/issues/62), [#55](https://github.com/nf-core/rnafusion/issues/55), [#53](https://github.com/nf-core/rnafusion/issues/53), [#51](https://github.com/nf-core/rnafusion/issues/51)
-* Added nextflow helper script `download-singularity-img.nf`
-* Added nextflow helper script `download-references.nf`
-* Added `Jenkinsfile` for in-house testing
+-   Added support for extra parameters for tools STAR-Fusion, FusionCatcher and fusion-report
+-   Added example configuration for `singularity` and `docker`
+-   Added [fusion-report](https://github.com/matq007/fusion-report) into the stack [#62](https://github.com/nf-core/rnafusion/issues/62), [#55](https://github.com/nf-core/rnafusion/issues/55), [#53](https://github.com/nf-core/rnafusion/issues/53), [#51](https://github.com/nf-core/rnafusion/issues/51)
+-   Added nextflow helper script `download-singularity-img.nf`
+-   Added nextflow helper script `download-references.nf`
+-   Added `Jenkinsfile` for in-house testing
 
 ### Changed
 
-* Updated installation of `FusionCatcher` (available now on bioconda)
+-   Updated installation of `FusionCatcher` (available now on bioconda)
 
 ### Fixed
 
-* Fixed empty symlinks (`input.X`) in fusion-report [#68](https://github.com/nf-core/rnafusion/issues/68)
-* Fixed FASTA issues [#60](https://github.com/nf-core/rnafusion/issues/60)
-* Fixed centralized nf-core/config [#64](https://github.com/nf-core/rnafusion/issues/64)
-* Fixed `scrape_software_versions.py` to parse tools versions correctly [#65](https://github.com/nf-core/rnafusion/issues/65)
+-   Fixed empty symlinks (`input.X`) in fusion-report [#68](https://github.com/nf-core/rnafusion/issues/68)
+-   Fixed FASTA issues [#60](https://github.com/nf-core/rnafusion/issues/60)
+-   Fixed centralized nf-core/config [#64](https://github.com/nf-core/rnafusion/issues/64)
+-   Fixed `scrape_software_versions.py` to parse tools versions correctly [#65](https://github.com/nf-core/rnafusion/issues/65)
 
 ### Removed
 
-* Removed `Singularity`
+-   Removed `Singularity`
 
 ---
 
diff --git a/CITATIONS.md b/CITATIONS.md
index 043c42b7..12e99bd0 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -10,51 +10,62 @@
 
 ## Pipeline tools
 
-* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
+-   [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
+
+-   [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
 
-* [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
     > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
 
-* [Arriba](https://github.com/suhrig/arriba)
+-   [Arriba](https://github.com/suhrig/arriba)
+
     > Uhrig S, Ellermann J, Walther T, Burkhardt P, Fröhlich M, Hutter B, Toprak UH, Neumann O, Stenzinger A, Scholl C, Fröhling S, Brors B. Accurate and efficient detection of gene fusions from RNA sequencing data.
-    Genome Research. 2021 Mar 31;448-460. doi: 10.1101/gr.257246.119. Epub 2021 Jan 13. PubMed PMID: 33441414; PubMed Central PMCID: PMC7919457.
+    > Genome Research. 2021 Mar 31;448-460. doi: 10.1101/gr.257246.119. Epub 2021 Jan 13. PubMed PMID: 33441414; PubMed Central PMCID: PMC7919457.
+
+-   [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
 
-* [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
     > Nicorici D, Satalan M, Edgren H, Kangaspeska S, Murumagi A, Kallioniemi O, Virtanen S, Kilkku O. FusionCatcher – a tool for finding somatic fusion genes in paired-end RNA-sequencing data. BioRxiv, 2014 Nov. doi: 10.1101/011650.
 
-* [Fusion-report](https://github.com/matq007/fusion-report)
+-   [Fusion-report](https://github.com/matq007/fusion-report)
+
     > Proks M, Genomic Profiling of a Comprehensive Nation-wide Collection of Childhood Solid Tumors, Master Thesis, Supervisors: Grøntved L, Díaz de Ståhl T, Nistér M, Ewels P, Garcia MU, Juhos S, University of Southern Denmark, 2019, unpublished.
 
-* [Kallisto](https://pachterlab.github.io/kallisto/)
+-   [Kallisto](https://pachterlab.github.io/kallisto/)
+
     > Bray NL, Pimentel H, Melsted P, Pachter L. Near-optimal probabilistic RNA-seq quantification. Nature Biotechnology 2016 Apr. 34, 525–527. doi:10.1038/nbt.3519. PMID: 27043002.
 
-* [Pizzly](https://github.com/pmelsted/pizzly)
+-   [Pizzly](https://github.com/pmelsted/pizzly)
     Melsted P, Hateley S, Joseph IC, Pimentel H, Bray N, Pachter L. Fusion detection and quantification by pseudoalignment. BioRxiv, 2017 Jul. doi: 10.1101/166322.
 
-* [SAMtools](https://pubmed.ncbi.nlm.nih.gov/19505943/)
+-   [SAMtools](https://pubmed.ncbi.nlm.nih.gov/19505943/)
+
     > Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PubMed PMID: 19505943; PubMed Central PMCID: PMC2723002.
 
-* [Squid](https://github.com/Kingsford-Group/squid)
+-   [Squid](https://github.com/Kingsford-Group/squid)
+
     > Ma C, Shao M, Kingsford C. SQUID: transcriptomic structural variation detection from RNA-seq. Genome Biol 2028 Apr. 19, 52. doi: 10.1186/s13059-018-1421-5. PubMed PMID: 29650026. PubMed Central PMCID: PMC5896115.
 
-* [STAR](https://pubmed.ncbi.nlm.nih.gov/23104886/)
+-   [STAR](https://pubmed.ncbi.nlm.nih.gov/23104886/)
+
     > Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner Bioinformatics. 2013 Jan 1;29(1):15-21. doi: 10.1093/bioinformatics/bts635. Epub 2012 Oct 25. PubMed PMID: 23104886; PubMed Central PMCID: PMC3530905.
 
-* [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)
+-   [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)
     > Haas BJ, Dobin A, Li B, Stransky N, Pochet N, Regev A. Accuracy assessment of fusion transcript detection via read-mapping and de novo fusion transcript assembly-based methods. Genome Biology 2019 Oct;20,213. doi: 10.1186/s13059-019-1842-9
 
 ## Software packaging/containerisation tools
 
-* [Anaconda](https://anaconda.com)
+-   [Anaconda](https://anaconda.com)
+
     > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web.
 
-* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/)
+-   [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/)
+
     > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506.
 
-* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/)
+-   [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/)
+
     > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671.
 
-* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241)
+-   [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241)
 
-* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/)
+-   [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/)
     > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675.
diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md
index f4fd052f..a47b923c 100644
--- a/CODE_OF_CONDUCT.md
+++ b/CODE_OF_CONDUCT.md
@@ -4,19 +4,19 @@
 
 In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of:
 
-- Age
-- Body size
-- Familial status
-- Gender identity and expression
-- Geographical location
-- Level of experience
-- Nationality and national origins
-- Native language
-- Physical and neurological ability
-- Race or ethnicity
-- Religion
-- Sexual identity and orientation
-- Socioeconomic status
+-   Age
+-   Body size
+-   Familial status
+-   Gender identity and expression
+-   Geographical location
+-   Level of experience
+-   Nationality and national origins
+-   Native language
+-   Physical and neurological ability
+-   Race or ethnicity
+-   Religion
+-   Sexual identity and orientation
+-   Socioeconomic status
 
 Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance.
 
@@ -48,38 +48,38 @@ Members of the core team or the safety officer who violate the CoC will be requi
 
 Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference:
 
-- Communicating with an official project email address.
-- Communicating with community members within the nf-core Slack channel.
-- Participating in hackathons organised by nf-core (both online and in-person events).
-- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence.
-- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc.
-- Representing nf-core on social media. This includes both official and personal accounts.
+-   Communicating with an official project email address.
+-   Communicating with community members within the nf-core Slack channel.
+-   Participating in hackathons organised by nf-core (both online and in-person events).
+-   Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence.
+-   Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc.
+-   Representing nf-core on social media. This includes both official and personal accounts.
 
 ## nf-core cares 😊
 
 nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order):
 
-- Ask for consent before sharing another community member’s personal information (including photographs) on social media.
-- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity.
-- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !)
-- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.)
-- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can)
-- Focus on what is best for the team and the community. (When in doubt, ask)
-- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn.
-- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!)
-- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**)
-- Take breaks when you feel like you need them.
-- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.)
+-   Ask for consent before sharing another community member’s personal information (including photographs) on social media.
+-   Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity.
+-   Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !)
+-   Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.)
+-   Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can)
+-   Focus on what is best for the team and the community. (When in doubt, ask)
+-   Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn.
+-   Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!)
+-   Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**)
+-   Take breaks when you feel like you need them.
+-   Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.)
 
 ## nf-core frowns on 😕
 
 The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces.
 
-- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom.
-- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online.
-- Spamming or trolling of individuals on social media.
-- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention.
-- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience.
+-   Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom.
+-   “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online.
+-   Spamming or trolling of individuals on social media.
+-   Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention.
+-   Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience.
 
 ### Online Trolling
 
@@ -99,13 +99,13 @@ All reports will be handled with utmost discretion and confidentially.
 
 ## Attribution and Acknowledgements
 
-- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4)
-- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition)
-- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/)
-- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla)
+-   The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4)
+-   The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition)
+-   The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/)
+-   The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla)
 
 ## Changelog
 
 ### v1.0 - March 12th, 2021
 
-- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC.
+-   Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC.
diff --git a/README.md b/README.md
index c4ab04e4..e464c6c3 100644
--- a/README.md
+++ b/README.md
@@ -20,24 +20,25 @@
 
 The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
 
-
 > **IMPORTANT: conda is not supported currently.** Run with singularity or docker.
 
 > GRCh38 is the only supported reference
 
-| Tool                                                                      |  Single-end reads  |  Version |
-| ------------------------------------------------------------------------- | :----------------: | :------: |
-| [Arriba](https://github.com/suhrig/arriba)                                |         :x:        |  `2.1.0` |
-| [FusionCatcher](https://github.com/ndaniel/fusioncatcher)                 | :white_check_mark: |  `1.33`  |
-| [Fusion-report](https://github.com/matq007/fusion-report)                 |          -         |  `2.1.5` |
-| [Pizzly](https://github.com/pmelsted/pizzly)                              |         :x:        | `0.37.3` |
-| [Squid](https://github.com/Kingsford-Group/squid)                         |         :x:        |   `1.5`  |
-| [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)                 | :white_check_mark: | `1.10.1` |
+| Tool                                                      |  Single-end reads  | Version  |
+| --------------------------------------------------------- | :----------------: | :------: |
+| [Arriba](https://github.com/suhrig/arriba)                |        :x:         | `2.1.0`  |
+| [FusionCatcher](https://github.com/ndaniel/fusioncatcher) | :white_check_mark: |  `1.33`  |
+| [Fusion-report](https://github.com/matq007/fusion-report) |         -          | `2.1.5`  |
+| [Pizzly](https://github.com/pmelsted/pizzly)              |        :x:         | `0.37.3` |
+| [Squid](https://github.com/Kingsford-Group/squid)         |        :x:         |  `1.5`   |
+| [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) | :white_check_mark: | `1.10.1` |
 
 ## Pipeline summary
 
 #### Build references
+
 `--build_references` triggers a parallel workflow to build all references
+
 1. Download ensembl fasta and gtf files
 2. Create STAR index
 3. Download arriba references
@@ -45,6 +46,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 5. Download pizzly references (kallisto index)
 6. Download and build STAR-fusion references
 7. Download fusion-report DBs
+
 #### Main workflow
 
 1. Input samplesheet check
@@ -52,30 +54,30 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 3. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
 4. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 5. Arriba subworkflow
-    * STAR alignment
-    * Samtool sort
-    * Samtool index
-    * [Arriba](https://github.com/suhrig/arriba) fusion detection
-    * [Arriba](https://github.com/suhrig/arriba) visualisation
+    - STAR alignment
+    - Samtool sort
+    - Samtool index
+    - [Arriba](https://github.com/suhrig/arriba) fusion detection
+    - [Arriba](https://github.com/suhrig/arriba) visualisation
 6. Pizzly subworkflow
-    * [Kallisto](https://pachterlab.github.io/kallisto/) quantification
-    * [Pizzly](https://github.com/pmelsted/pizzly) fusion detection
+    - [Kallisto](https://pachterlab.github.io/kallisto/) quantification
+    - [Pizzly](https://github.com/pmelsted/pizzly) fusion detection
 7. Squid subworkflow
-    * [STAR](https://github.com/alexdobin/STAR) alignment
-    * [Samtools view](http://www.htslib.org/): convert sam output from STAR to bam
-    * [Samtools sort](http://www.htslib.org/): bam output from STAR
-    * [Squid](https://github.com/Kingsford-Group/squid) fusion detection
-    * [Squid](https://github.com/Kingsford-Group/squid) annotate
+    - [STAR](https://github.com/alexdobin/STAR) alignment
+    - [Samtools view](http://www.htslib.org/): convert sam output from STAR to bam
+    - [Samtools sort](http://www.htslib.org/): bam output from STAR
+    - [Squid](https://github.com/Kingsford-Group/squid) fusion detection
+    - [Squid](https://github.com/Kingsford-Group/squid) annotate
 8. STAR-fusion subworkflow
-    * [STAR](https://github.com/alexdobin/STAR) alignment
-    * [STAR-fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
+    - [STAR](https://github.com/alexdobin/STAR) alignment
+    - [STAR-fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
 9. Fusioncatcher subworkflow
-    * [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
+    - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
 10. Fusion-report subworkflow
-    * Merge all fusions detected by the different tools
-    * [Fusion-report](https://github.com/matq007/fusion-report)
+    - Merge all fusions detected by the different tools
+    - [Fusion-report](https://github.com/matq007/fusion-report)
 11. FusionInspector subworkflow
-    * [FusionInspector](https://github.com/FusionInspector/FusionInspector)
+    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)
 
 <!-- TODO Add QC steps, MultiQC details -->
 
@@ -93,10 +95,10 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 
     Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
 
-    > * The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
-    > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
-    > * If you are using `singularity` and are persistently observing issues downloading Singularity images directly due to timeout or network issues, then you can use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, you can use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
-    > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
+    > - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
+    > - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
+    > - If you are using `singularity` and are persistently observing issues downloading Singularity images directly due to timeout or network issues, then you can use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, you can use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
+    > - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
 
 4. Start running your own analysis!
 
@@ -114,7 +116,7 @@ nf-core/rnafusion was originally written by Martin Proks, @praveenraj2018, on th
 
 We thank the following people for their extensive assistance in the development of this pipeline:
 
-* Annick Renevey (@rannick)
+-   Annick Renevey (@rannick)
 
 ## Contributions and Support
 
@@ -125,7 +127,8 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 ## Citations
 
 <!-- TODO update zenodo after release -->
-If you use  nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)
+
+If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)
 
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
 
diff --git a/assets/email_template.html b/assets/email_template.html
index efcd0eaa..82aea34e 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -1,53 +1,134 @@
 <html>
-<head>
-  <meta charset="utf-8">
-  <meta http-equiv="X-UA-Compatible" content="IE=edge">
-  <meta name="viewport" content="width=device-width, initial-scale=1">
+  <head>
+    <meta charset="utf-8" />
+    <meta http-equiv="X-UA-Compatible" content="IE=edge" />
+    <meta name="viewport" content="width=device-width, initial-scale=1" />
 
-  <meta name="description" content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community.">
-  <title>nf-core/rnafusion Pipeline Report</title>
-</head>
-<body>
-<div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto;">
+    <meta
+      name="description"
+      content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community."
+    />
+    <title>nf-core/rnafusion Pipeline Report</title>
+  </head>
+  <body>
+    <div
+      style="
+        font-family: Helvetica, Arial, sans-serif;
+        padding: 30px;
+        max-width: 800px;
+        margin: 0 auto;
+      "
+    >
+      <img src="cid:nfcorepipelinelogo" />
 
-<img src="cid:nfcorepipelinelogo">
+      <h1>nf-core/rnafusion v${version}</h1>
+      <h2>Run Name: $runName</h2>
 
-<h1>nf-core/rnafusion v${version}</h1>
-<h2>Run Name: $runName</h2>
-
-<% if (!success){
-    out << """
-    <div style="color: #a94442; background-color: #f2dede; border-color: #ebccd1; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
-        <h4 style="margin-top:0; color: inherit;">nf-core/rnafusion execution completed unsuccessfully!</h4>
-        <p>The exit status of the task that caused the workflow execution to fail was: <code>$exitStatus</code>.</p>
+      <% if (!success){ out << """
+      <div
+        style="
+          color: #a94442;
+          background-color: #f2dede;
+          border-color: #ebccd1;
+          padding: 15px;
+          margin-bottom: 20px;
+          border: 1px solid transparent;
+          border-radius: 4px;
+        "
+      >
+        <h4 style="margin-top: 0; color: inherit">
+          nf-core/rnafusion execution completed unsuccessfully!
+        </h4>
+        <p>
+          The exit status of the task that caused the workflow execution to fail
+          was: <code>$exitStatus</code>.
+        </p>
         <p>The full error message was:</p>
-        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0;">${errorReport}</pre>
-    </div>
-    """
-} else {
-    out << """
-    <div style="color: #3c763d; background-color: #dff0d8; border-color: #d6e9c6; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
+        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0">
+${errorReport}</pre
+        >
+      </div>
+      """ } else { out << """
+      <div
+        style="
+          color: #3c763d;
+          background-color: #dff0d8;
+          border-color: #d6e9c6;
+          padding: 15px;
+          margin-bottom: 20px;
+          border: 1px solid transparent;
+          border-radius: 4px;
+        "
+      >
         nf-core/rnafusion execution completed successfully!
-    </div>
-    """
-}
-%>
+      </div>
+      """ } %>
 
-<p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
-<p>The command used to launch the workflow was as follows:</p>
-<pre style="white-space: pre-wrap; overflow: visible; background-color: #ededed; padding: 15px; border-radius: 4px; margin-bottom:30px;">$commandLine</pre>
+      <p>
+        The workflow was completed at <strong>$dateComplete</strong> (duration:
+        <strong>$duration</strong>)
+      </p>
+      <p>The command used to launch the workflow was as follows:</p>
+      <pre
+        style="
+          white-space: pre-wrap;
+          overflow: visible;
+          background-color: #ededed;
+          padding: 15px;
+          border-radius: 4px;
+          margin-bottom: 30px;
+        "
+      >
+$commandLine</pre
+      >
 
-<h3>Pipeline Configuration:</h3>
-<table style="width:100%; max-width:100%; border-spacing: 0; border-collapse: collapse; border:0; margin-bottom: 30px;">
-    <tbody style="border-bottom: 1px solid #ddd;">
-        <% out << summary.collect{ k,v -> "<tr><th style='text-align:left; padding: 8px 0; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'>$k</th><td style='text-align:left; padding: 8px; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'><pre style='white-space: pre-wrap; overflow: visible;'>$v</pre></td></tr>" }.join("\n") %>
-    </tbody>
-</table>
+      <h3>Pipeline Configuration:</h3>
+      <table
+        style="
+          width: 100%;
+          max-width: 100%;
+          border-spacing: 0;
+          border-collapse: collapse;
+          border: 0;
+          margin-bottom: 30px;
+        "
+      >
+        <tbody style="border-bottom: 1px solid #ddd">
+          <% out << summary.collect{ k,v -> "
+          <tr>
+            <th
+              style="
+                text-align: left;
+                padding: 8px 0;
+                line-height: 1.42857143;
+                vertical-align: top;
+                border-top: 1px solid #ddd;
+              "
+            >
+              $k
+            </th>
+            <td
+              style="
+                text-align: left;
+                padding: 8px;
+                line-height: 1.42857143;
+                vertical-align: top;
+                border-top: 1px solid #ddd;
+              "
+            >
+              <pre style="white-space: pre-wrap; overflow: visible">$v</pre>
+            </td>
+          </tr>
+          " }.join("\n") %>
+        </tbody>
+      </table>
 
-<p>nf-core/rnafusion</p>
-<p><a href="https://github.com/nf-core/rnafusion">https://github.com/nf-core/rnafusion</a></p>
-
-</div>
-
-</body>
+      <p>nf-core/rnafusion</p>
+      <p>
+        <a href="https://github.com/nf-core/rnafusion"
+          >https://github.com/nf-core/rnafusion</a
+        >
+      </p>
+    </div>
+  </body>
 </html>
diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml
index 611287cc..ce53881a 100644
--- a/assets/multiqc_config.yaml
+++ b/assets/multiqc_config.yaml
@@ -1,11 +1,11 @@
 report_comment: >
-    This report has been generated by the <a href="https://github.com/nf-core/rnafusion" target="_blank">nf-core/rnafusion</a>
-    analysis pipeline. For information about how to interpret these results, please see the
-    <a href="https://nf-co.re/rnafusion" target="_blank">documentation</a>.
+  This report has been generated by the <a href="https://github.com/nf-core/rnafusion" target="_blank">nf-core/rnafusion</a>
+  analysis pipeline. For information about how to interpret these results, please see the
+  <a href="https://nf-co.re/rnafusion" target="_blank">documentation</a>.
 report_section_order:
-    software_versions:
-        order: -1000
-    nf-core-rnafusion-summary:
-        order: -1001
+  software_versions:
+    order: -1000
+  nf-core-rnafusion-summary:
+    order: -1001
 
 export_plots: true
diff --git a/assets/schema_input.json b/assets/schema_input.json
index 435d174a..aff11de1 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -31,9 +31,6 @@
                 ]
             }
         },
-        "required": [
-            "sample",
-            "fastq_1"
-        ]
+        "required": ["sample", "fastq_1"]
     }
 }
diff --git a/docs/README.md b/docs/README.md
index 08dec85e..f8520ea5 100644
--- a/docs/README.md
+++ b/docs/README.md
@@ -2,9 +2,9 @@
 
 The nf-core/rnafusion documentation is split into the following pages:
 
-* [Usage](usage.md)
-    * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
-* [Output](output.md)
-    * An overview of the different results produced by the pipeline and how to interpret them.
+-   [Usage](usage.md)
+    -   An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
+-   [Output](output.md)
+    -   An overview of the different results produced by the pipeline and how to interpret them.
 
 You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re)
diff --git a/docs/output.md b/docs/output.md
index c38a2a7a..800a4287 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -10,56 +10,56 @@ The directories listed below will be created in the results directory after the
 
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
-* [Download and build references](#references) - Build references needed to run the rest of the pipeline
-* [STAR](#star) - Alignment for arriba, squid and STAR-fusion
-* [Cat](#cat) - Concatenated fastq files per sample ID
-* [Arriba](#arriba) - Arriba fusion detection
-* [Pizzly](#pizzly) - Pizzly fusion detection
-* [Squid](#squid) - Squid fusion detection
-* [STAR-fusion](#starfusion) - STAR-fusion fusion detection
-* [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
-* [Samtools](#samtools) - SAM/BAM file manipulation
-* [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report
-* [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman and FusionGBD databases
-* [FusionInspector](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
-* [Qualimap](#qualimap) - quality control of alignment
-* [Picard](#picard) - collect metrics
-* [FastQC](#fastqc) - Raw read quality control
-* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
-* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
-
+-   [Download and build references](#references) - Build references needed to run the rest of the pipeline
+-   [STAR](#star) - Alignment for arriba, squid and STAR-fusion
+-   [Cat](#cat) - Concatenated fastq files per sample ID
+-   [Arriba](#arriba) - Arriba fusion detection
+-   [Pizzly](#pizzly) - Pizzly fusion detection
+-   [Squid](#squid) - Squid fusion detection
+-   [STAR-fusion](#starfusion) - STAR-fusion fusion detection
+-   [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
+-   [Samtools](#samtools) - SAM/BAM file manipulation
+-   [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report
+-   [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman and FusionGBD databases
+-   [FusionInspector](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
+-   [Qualimap](#qualimap) - quality control of alignment
+-   [Picard](#picard) - collect metrics
+-   [FastQC](#fastqc) - Raw read quality control
+-   [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
+-   [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ### Download and build references
 
 <details markdown="1">
 <summary>Output files</summary>
 
-* `genomes_base/`
-    * `arriba`
-        * `blacklist_hg38_GRCh38_v2.1.0.tsv.gz`
-        * `protein_domains_hg38_GRCh38_v2.1.0.gff3`
-        * `cytobands_hg38_GRCh38_v2.1.0.tsv`
-    * `ensembl`
-        * `Homo_sapiens.GRCh38.{ensembl_version}.all.fa`
-        * `Homo_sapiens.GRCh38.{ensembl_version}.cdna.all.fa.gz`
-        * `Homo_sapiens.GRCh38.{ensembl_version}.gtf`
-        * `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf`
-        * `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf.refflat`
-    * `fusioncatcher`
-        * `human_v<version>` - dir with all references for fusioncatcher
-    * `fusion_report_db`
-        * `cosmic.db`
-        * `fusiongdb.db`
-        * `fusiongdb2.db`
-        * `mitelman.db`
-    * `pizzly`
-        * `kallisto` - file containing the kallisto index
-    * `star` - dir with STAR index
-    * `starfusion`
-        * files and dirs used to build the index
-        * `ctat_genome_lib_build_dir` - dir containing the index
+-   `genomes_base/`
+    -   `arriba`
+        -   `blacklist_hg38_GRCh38_v2.1.0.tsv.gz`
+        -   `protein_domains_hg38_GRCh38_v2.1.0.gff3`
+        -   `cytobands_hg38_GRCh38_v2.1.0.tsv`
+    -   `ensembl`
+        -   `Homo_sapiens.GRCh38.{ensembl_version}.all.fa`
+        -   `Homo_sapiens.GRCh38.{ensembl_version}.cdna.all.fa.gz`
+        -   `Homo_sapiens.GRCh38.{ensembl_version}.gtf`
+        -   `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf`
+        -   `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf.refflat`
+    -   `fusioncatcher`
+        -   `human_v<version>` - dir with all references for fusioncatcher
+    -   `fusion_report_db`
+        -   `cosmic.db`
+        -   `fusiongdb.db`
+        -   `fusiongdb2.db`
+        -   `mitelman.db`
+    -   `pizzly`
+        -   `kallisto` - file containing the kallisto index
+    -   `star` - dir with STAR index
+    -   `starfusion`
+        -   files and dirs used to build the index
+        -   `ctat_genome_lib_build_dir` - dir containing the index
 
 (Only files or folders used by the pipeline are mentioned explicitly.)
+
 </details>
 
 ### STAR
@@ -135,21 +135,21 @@ For STAR-fusion with the parameters:
 <details markdown="1">
 <summary>Output files</summary>
 
-* `star_for_<tool>`
-    * **Common**
-    * `<sample_id>.Log.final.out`
-    * `<sample_id>.Log.progress.out`
-    * `<sample_id>.SJ.out.tab`
-    * **For arriba:**
-    * `<sample_id>.Aligned.out.bam`
-    * **For squid:**
-    * `<sample_id>.Aligned.sortedByCoord.out.bam`
-    * `<sample_id>.Chimeric.out.sam`
-    * `<sample_id>.unmapped_1.fastq.gz`
-    * `<sample_id>.unmapped_2.fastq.gz`
-    * **For starfusion:**
-    * `<sample_id>.Aligned.sortedByCoord.out.bam`
-    * `<sample_id>.Chimeric.out.junction`
+-   `star_for_<tool>`
+_ **Common**
+_ `<sample_id>.Log.final.out`
+_ `<sample_id>.Log.progress.out`
+_ `<sample_id>.SJ.out.tab`
+_ **For arriba:**
+_ `<sample_id>.Aligned.out.bam`
+_ **For squid:**
+_ `<sample_id>.Aligned.sortedByCoord.out.bam`
+_ `<sample_id>.Chimeric.out.sam`
+_ `<sample_id>.unmapped_1.fastq.gz`
+_ `<sample_id>.unmapped_2.fastq.gz`
+_ **For starfusion:**
+_ `<sample_id>.Aligned.sortedByCoord.out.bam`
+_ `<sample_id>.Chimeric.out.junction`
 </details>
 
 ### Cat
@@ -159,31 +159,34 @@ Cat is used to concatenate fastq files belonging to the same sample.
 <details markdown="1">
 <summary>Output files</summary>
 
-* `cat`
-    * `<sample_id>_1.merged.fastq.gz`
-    * `<sample_id>_2.merged.fastq.gz`
+-   `cat`
+    -   `<sample_id>_1.merged.fastq.gz`
+    -   `<sample_id>_2.merged.fastq.gz`
 
 </details>
 
 ### Arriba
 
 Arriba is used for i) detect fusion and ii) output a PDF report for the fusions found (visualisation):
+
 #### Detection
+
 <details markdown="1">
 <summary>Output files</summary>
 
-* `arriba`
-    * `<sample_id>.arriba.fusions.tsv` - contains the identified fusions
-    * `<sample_id>.arriba.fusions.discarded.tsv`
+-   `arriba`
+    -   `<sample_id>.arriba.fusions.tsv` - contains the identified fusions
+    -   `<sample_id>.arriba.fusions.discarded.tsv`
 
 </details>
 
 #### Visualisation
+
 <details markdown="1">
 <summary>Output files</summary>
 
-* `arriba_visualisation`
-    * `<sample_id>.pdf`
+-   `arriba_visualisation`
+    -   `<sample_id>.pdf`
 
 </details>
 
@@ -196,8 +199,8 @@ The first step of the pizzly workflow is to run `kallisto quant`:
 <details markdown="1">
 <summary>Output files</summary>
 
-* `kallisto`
-    * `<sample_id>.kallisto_quant.fusions.txt`
+-   `kallisto`
+    -   `<sample_id>.kallisto_quant.fusions.txt`
 
 </details>
 
@@ -217,13 +220,12 @@ Pizzly uses the following arguments:
 <details markdown="1">
 <summary>Output files</summary>
 
-* `pizzly`
-    * `<sample_id>.pizzly.txt` - contains the identified fusions
-    * `<sample_id>.pizzly.unfiltered.json`
+-   `pizzly`
+    -   `<sample_id>.pizzly.txt` - contains the identified fusions
+    -   `<sample_id>.pizzly.unfiltered.json`
 
 </details>
 
-
 ### Squid
 
 Squid is run in two steps: i) fusion detection and ii) fusion annotation but the output is in a common `squid` directory.
@@ -231,9 +233,9 @@ Squid is run in two steps: i) fusion detection and ii) fusion annotation but the
 <details markdown="1">
 <summary>Output files</summary>
 
-* `squid`
-    * `<sample_id>.squid.fusions_sv.txt` - contains the identified fusions
-    * `<sample_id>.squid.fusions.annotated.txt`- contains the identified fusions annotatedvi
+-   `squid`
+    -   `<sample_id>.squid.fusions_sv.txt` - contains the identified fusions
+    -   `<sample_id>.squid.fusions.annotated.txt`- contains the identified fusions annotatedvi
 
 </details>
 
@@ -242,10 +244,10 @@ Squid is run in two steps: i) fusion detection and ii) fusion annotation but the
 <details markdown="1">
 <summary>Output files</summary>
 
-* `starfusion`
-    * `<sample_id>.starfusion.fusion_predictions.tsv` - contains the identified fusions
-    * `<sample_id>.starfusion.abridged.tsv`
-    * `- contains the identified fusions.starfusion.abridged.coding_effect.tsv`
+-   `starfusion`
+    -   `<sample_id>.starfusion.fusion_predictions.tsv` - contains the identified fusions
+    -   `<sample_id>.starfusion.abridged.tsv`
+    -   `- contains the identified fusions.starfusion.abridged.coding_effect.tsv`
 
 </details>
 
@@ -254,10 +256,9 @@ Squid is run in two steps: i) fusion detection and ii) fusion annotation but the
 <details markdown="1">
 <summary>Output files</summary>
 
-* `fusioncatcher`
-    * `<sample_id>.fusioncatcher.fusion-genes.txt`
-    * `<sample_id>.fusioncatcher.summary.txt`
-    * `<sample_id>.fusioncatcher.log`
+-   `fusioncatcher`
+_ `<sample_id>.fusioncatcher.fusion-genes.txt`
+_ `<sample_id>.fusioncatcher.summary.txt` \* `<sample_id>.fusioncatcher.log`
 </details>
 
 ### Samtools
@@ -269,8 +270,8 @@ Samtools view is used to convert the chimeric SAM output from STAR_FOR_SQUID to
 <details markdown="1">
 <summary>Output files</summary>
 
-*  `samtools_view_for_squid`
-    * `<sample_id>_chimeric.bam` - sorted BAM file
+-   `samtools_view_for_squid`
+    -   `<sample_id>_chimeric.bam` - sorted BAM file
 
 </details>
 
@@ -281,8 +282,8 @@ Samtools sort is used to sort BAM files from STAR_FOR_ARRIBA (for arriba visuali
 <details markdown="1">
 <summary>Output files</summary>
 
-*  `samtools_sort_for_<arriba/squid>`
-    * `<sample_id>(_chimeric)_sorted.bam` - sorted BAM file
+-   `samtools_sort_for_<arriba/squid>`
+    -   `<sample_id>(_chimeric)_sorted.bam` - sorted BAM file
 
 </details>
 
@@ -293,8 +294,8 @@ Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visua
 <details markdown="1">
 <summary>Output files</summary>
 
-*  `samtools_for_<arriba/qc>`
-    * `<sample_id>.(Aligned.sortedByCoord).out.bam.bai` -
+-   `samtools_for_<arriba/qc>`
+    -   `<sample_id>.(Aligned.sortedByCoord).out.bam.bai` -
 
 </details>
 
@@ -303,12 +304,12 @@ Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visua
 <details markdown="1">
 <summary>Output files</summary>
 
-* `fusionreport`
-    * <sample_id>
-        * `<sample_id>.fusionreport.tsv`
-        * `<sample_id>.fusionreport_filtered.tsv`
-        * `index.html` - general report for all filtered fusions
-        * `<fusion>.html` - specific report for each filtered fusion
+-   `fusionreport`
+    -   <sample_id>
+        -   `<sample_id>.fusionreport.tsv`
+        -   `<sample_id>.fusionreport_filtered.tsv`
+        -   `index.html` - general report for all filtered fusions
+        -   `<fusion>.html` - specific report for each filtered fusion
 
 </details>
 
@@ -317,10 +318,10 @@ Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visua
 <details markdown="1">
 <summary>Output files</summary>
 
-*  `fusioninspector`
-    * `<sample_id>.fusion_inspector_web.html` - visualisation report described in details [here](https://github.com/FusionInspector/FusionInspector/wiki/FusionInspector-Visualizations)
-    * `FusionInspector.log`
-    * `<sample_id>.FusionInspector.fusions.abridged.tsv`
+-   `fusioninspector`
+    -   `<sample_id>.fusion_inspector_web.html` - visualisation report described in details [here](https://github.com/FusionInspector/FusionInspector/wiki/FusionInspector-Visualizations)
+    -   `FusionInspector.log`
+    -   `<sample_id>.FusionInspector.fusions.abridged.tsv`
 
 </details>
 
@@ -329,26 +330,26 @@ Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visua
 <details markdown="1">
 <summary>Output files</summary>
 
-*  `qualimap`
-    * `qualimapReport.html` - HTML report
-    * `rnaseq_qc_results.txt` - TXT results
-    * `css` - dir for html style
-    * `images_qualimapReport`- dir for html images
-    * `raw_data_qualimapReport` - dir for html raw data
-
+-   `qualimap`
+    -   `qualimapReport.html` - HTML report
+    -   `rnaseq_qc_results.txt` - TXT results
+    -   `css` - dir for html style
+    -   `images_qualimapReport`- dir for html images
+    -   `raw_data_qualimapReport` - dir for html raw data
 
 </details>
 
 ### Picard
 
 Picard CollectRnaMetrics and picard MarkDuplicates share the same outpur directory.
+
 <details markdown="1">
 <summary>Output files</summary>
 
-*  `picard`
-    * `<sample_id>.MarkDuplicates.metrics.txt` - metrics from CollectRnaMetrics
-    * `<sample_id>_rna_metrics.txt` - metrics from MarkDuplicates
-    * `<sample_id>.bam` - BAM file with marked duplicates
+-   `picard`
+    -   `<sample_id>.MarkDuplicates.metrics.txt` - metrics from CollectRnaMetrics
+    -   `<sample_id>_rna_metrics.txt` - metrics from MarkDuplicates
+    -   `<sample_id>.bam` - BAM file with marked duplicates
 
 </details>
 
@@ -357,9 +358,9 @@ Picard CollectRnaMetrics and picard MarkDuplicates share the same outpur directo
 <details markdown="1">
 <summary>Output files</summary>
 
-* `fastqc/`
-    * `*_fastqc.html`: FastQC report containing quality metrics.
-    * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
+-   `fastqc/`
+    -   `*_fastqc.html`: FastQC report containing quality metrics.
+    -   `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
 </details>
 
@@ -378,10 +379,10 @@ Picard CollectRnaMetrics and picard MarkDuplicates share the same outpur directo
 <details markdown="1">
 <summary>Output files</summary>
 
-* `multiqc/`
-    * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
-    * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
-    * `multiqc_plots/`: directory containing static images from the report in various formats.
+-   `multiqc/`
+    -   `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
+    -   `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
+    -   `multiqc_plots/`: directory containing static images from the report in various formats.
 
 </details>
 
@@ -394,10 +395,10 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ
 <details markdown="1">
 <summary>Output files</summary>
 
-* `pipeline_info/`
-    * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
-    * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
-    * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
+-   `pipeline_info/`
+    -   Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
+    -   Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
+    -   Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
 
 </details>
 
diff --git a/docs/usage.md b/docs/usage.md
index 66d5e721..b7bdeb16 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -3,23 +3,24 @@
 ## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/rnafusion/usage](https://nf-co.re/rnafusion/usage)
 
 > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._
+
 ## Introduction
 
 The pipeline is divided into two parts:
 
 1. Downloading and building the references, using `--build_references`: done only once and after each update.
 2. Detecting fusions using (any combination of) the following tools:
-    * arriba
-    * fusioncatcher
-    * pizzly
-    * squid
-    * starfusion
+    - arriba
+    - fusioncatcher
+    - pizzly
+    - squid
+    - starfusion
 3. QC and visualisation tools
-    * Fastqc
-    * MultiQC
-    * arriba visualisation (for fusion detected by arriba only)
-    * fusion-report
-    * fusionInspector
+    - Fastqc
+    - MultiQC
+    - arriba visualisation (for fusion detected by arriba only)
+    - fusion-report
+    - fusionInspector
 
 ### Prerequisite: download and build references
 
@@ -33,6 +34,7 @@ nextflow run nf-core/rnafusion \
 ```
 
 References for the different tools can also be downloaded separately:
+
 ```bash
 nextflow run nf-core/rnafusion \
 --build_references --<tool> \
@@ -54,6 +56,7 @@ Visualisation tools will be run on all fusion detected.
 #### Optional manual feed-in of fusion files
 
 It is possible to give the output of each tool manually using the argument: `--<tool>_fusions PATH/TO/FUSION/FILE`.
+
 ## Samplesheet input
 
 You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below.
@@ -91,7 +94,7 @@ TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,forward
 ```
 
 | Column         | Description                                                                                                                                                                            |
-|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
+| -------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
 | `sample`       | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). |
 | `fastq_1`      | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
 | `fastq_2`      | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
@@ -153,21 +156,21 @@ They are loaded in sequence, so later profiles can overwrite earlier profiles.
 
 If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended.
 
-* `docker`
-    * A generic configuration profile to be used with [Docker](https://docker.com/)
-* `singularity`
-    * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
-* `podman`
-    * A generic configuration profile to be used with [Podman](https://podman.io/)
-* `shifter`
-    * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
-* `charliecloud`
-    * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
-* `conda`
-    * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
-* `test`
-    * A profile with a complete configuration for automated testing
-    * Includes links to test data so needs no other parameters
+-   `docker`
+    -   A generic configuration profile to be used with [Docker](https://docker.com/)
+-   `singularity`
+    -   A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
+-   `podman`
+    -   A generic configuration profile to be used with [Podman](https://podman.io/)
+-   `shifter`
+    -   A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
+-   `charliecloud`
+    -   A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
+-   `conda`
+    -   A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
+-   `test`
+    -   A profile with a complete configuration for automated testing
+    -   Includes links to test data so needs no other parameters
 
 ### `-resume`
 
@@ -234,6 +237,7 @@ process {
 
 > **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden.
 > If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
+
 ### Updating containers
 
 The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`.
@@ -242,7 +246,7 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
 2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
 3. Create the custom config accordingly:
 
-    * For Docker:
+    - For Docker:
 
         ```nextflow
         process {
@@ -252,7 +256,7 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
         }
         ```
 
-    * For Singularity:
+    - For Singularity:
 
         ```nextflow
         process {
@@ -262,7 +266,7 @@ The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementatio
         }
         ```
 
-    * For Conda:
+    - For Conda:
 
         ```nextflow
         process {
diff --git a/modules.json b/modules.json
index b7ee22d9..5c17792d 100644
--- a/modules.json
+++ b/modules.json
@@ -41,4 +41,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/local/fusioncatcher/detect/meta.yml b/modules/local/fusioncatcher/detect/meta.yml
index 8081142b..7c8ee425 100644
--- a/modules/local/fusioncatcher/detect/meta.yml
+++ b/modules/local/fusioncatcher/detect/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://github.com/ndaniel/fusioncatcher/wiki
       tool_dev_url: https://github.com/ndaniel/fusioncatcher
       doi: "10.1101/011650v1"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - meta:
@@ -45,9 +45,9 @@ output:
       description: Log of fusion results
       pattern: "*.fusioncatcher.log"
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 
 authors:
   - "@praveenraj2018. @rannick"
diff --git a/modules/local/fusioncatcher/download/meta.yml b/modules/local/fusioncatcher/download/meta.yml
index b24b5e82..40421a4e 100644
--- a/modules/local/fusioncatcher/download/meta.yml
+++ b/modules/local/fusioncatcher/download/meta.yml
@@ -9,13 +9,13 @@ tools:
       documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
       tool_dev_url: https://github.com/ndaniel/fusioncatcher/
       doi: "10.1101/011650"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 output:
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
   - reference:
       type: directory
       description: Path to fusioncatcher references
diff --git a/modules/local/fusioninspector/meta.yml b/modules/local/fusioninspector/meta.yml
index 98f86a1c..cc03239b 100644
--- a/modules/local/fusioninspector/meta.yml
+++ b/modules/local/fusioninspector/meta.yml
@@ -28,9 +28,9 @@ input:
 
 output:
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
   - reference:
       type: directory
       description: Genome resource path
diff --git a/modules/local/fusionreport/detect/meta.yml b/modules/local/fusionreport/detect/meta.yml
index 72101495..f3d5cd88 100644
--- a/modules/local/fusionreport/detect/meta.yml
+++ b/modules/local/fusionreport/detect/meta.yml
@@ -8,7 +8,7 @@ tools:
       homepage: https://github.com/matq007/fusion-report
       documentation: https://matq007.github.io/fusion-report/#/
       doi: "10.1101/011650"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - meta:
@@ -43,13 +43,13 @@ input:
 
 output:
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
   - fusion_list:
-        type: file
-        description: File containing the summary of all fusions fed-in
-        pattern: "*.tsv"
+      type: file
+      description: File containing the summary of all fusions fed-in
+      pattern: "*.tsv"
   - report:
       type: file
       description: HTML files
diff --git a/modules/local/fusionreport/download/meta.yml b/modules/local/fusionreport/download/meta.yml
index d4bab11a..21a15a89 100644
--- a/modules/local/fusionreport/download/meta.yml
+++ b/modules/local/fusionreport/download/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
       tool_dev_url: https://github.com/ndaniel/fusioncatcher/
       doi: "10.1101/011650"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - username:
@@ -23,9 +23,9 @@ input:
 
 output:
   - versions:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
   - reference:
       type: directory
       description: directory containing the genome resource files required for fusioncatcher
diff --git a/modules/local/kallisto/quant/meta.yml b/modules/local/kallisto/quant/meta.yml
index 836bf9ff..eef0a2b0 100644
--- a/modules/local/kallisto/quant/meta.yml
+++ b/modules/local/kallisto/quant/meta.yml
@@ -8,7 +8,7 @@ tools:
       documentation: https://pachterlab.github.io/kallisto/manual
       tool_dev_url: https://github.com/pachterlab/kallisto
       doi: ""
-      licence: ['BSD-2-Clause']
+      licence: ["BSD-2-Clause"]
 
 input:
   - fasta:
diff --git a/modules/local/pizzly/detect/meta.yml b/modules/local/pizzly/detect/meta.yml
index 97bf8f09..930b3a62 100644
--- a/modules/local/pizzly/detect/meta.yml
+++ b/modules/local/pizzly/detect/meta.yml
@@ -10,7 +10,7 @@ tools:
       documentation: https://github.com/pmelsted/pizzly
       tool_dev_url: https://github.com/pmelsted/pizzly
       doi: ""
-      licence: ['BSD-2-Clause']
+      licence: ["BSD-2-Clause"]
 
 input:
   - fasta:
diff --git a/modules/local/squid/annotate/meta.yml b/modules/local/squid/annotate/meta.yml
index ed5e6048..e1a1f0d2 100644
--- a/modules/local/squid/annotate/meta.yml
+++ b/modules/local/squid/annotate/meta.yml
@@ -10,7 +10,7 @@ tools:
       documentation: https://github.com/Kingsford-Group/squid
       tool_dev_url: https://github.com/Kingsford-Group/squid
       doi: ""
-      licence: ['BSD-3-Clause']
+      licence: ["BSD-3-Clause"]
 
 input:
   - fusions:
diff --git a/modules/local/squid/detect/meta.yml b/modules/local/squid/detect/meta.yml
index db1cc2bb..a7f1e61a 100644
--- a/modules/local/squid/detect/meta.yml
+++ b/modules/local/squid/detect/meta.yml
@@ -10,10 +10,9 @@ tools:
       documentation: https://github.com/Kingsford-Group/squid
       tool_dev_url: https://github.com/Kingsford-Group/squid
       doi: ""
-      licence: ['BSD-3-Clause']
+      licence: ["BSD-3-Clause"]
 
 input:
-
   - meta:
       type: map
       description: |
diff --git a/modules/local/starfusion/detect/meta.yml b/modules/local/starfusion/detect/meta.yml
index 098c6596..136facba 100644
--- a/modules/local/starfusion/detect/meta.yml
+++ b/modules/local/starfusion/detect/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki
       tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion/releases
       doi: "10.1101/120295v1"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - meta:
diff --git a/modules/local/starfusion/download/meta.yml b/modules/local/starfusion/download/meta.yml
index 37f71f06..dc7fee75 100644
--- a/modules/local/starfusion/download/meta.yml
+++ b/modules/local/starfusion/download/meta.yml
@@ -9,7 +9,7 @@ tools:
       documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki/installing-star-fusion
       tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion
       doi: "10.1186/s13059-019-1842-9"
-      licence: ['GPL v3']
+      licence: ["GPL v3"]
 
 input:
   - genome_version:
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
index 5b5b8a60..60b546a0 100644
--- a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
+++ b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
@@ -8,7 +8,7 @@ tools:
       description: Custom module used to dump software versions within the nf-core pipeline template
       homepage: https://github.com/nf-core/tools
       documentation: https://github.com/nf-core/tools
-      licence: ['MIT']
+      licence: ["MIT"]
 input:
   - versions:
       type: file
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 0aadc869..fd7f3a44 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -10,9 +10,7 @@
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": [
-                "input"
-            ],
+            "required": ["input"],
             "properties": {
                 "input": {
                     "type": "string",
@@ -409,4 +407,4 @@
             "$ref": "#/definitions/generic_options"
         }
     ]
-}
\ No newline at end of file
+}

From 89be3bb5499258ab7797dc0960f028c52a5dac11 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 14:04:53 +0100
Subject: [PATCH 232/359] add .nf-core.yml

---
 .nf-core.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.nf-core.yml b/.nf-core.yml
index c212cb4a..6151892a 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -2,3 +2,4 @@ lint:
   files_unchanged:
     # Added to ignore linting error: actions/setup-node@v1 until fixed release
     - .github/workflows/linting.yml
+repository_type: pipeline

From 0c730a6c8bfbea9a941a4b2a2fcee63944b99cdf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 14:07:56 +0100
Subject: [PATCH 233/359] nf-core lint fix unchanged

---
 .github/CONTRIBUTING.md               | 15 +++--
 .github/PULL_REQUEST_TEMPLATE.md      | 21 ++++---
 .github/workflows/branch.yml          |  3 +-
 .github/workflows/linting_comment.yml |  1 +
 .markdownlint.yml                     | 14 -----
 CODE_OF_CONDUCT.md                    | 80 +++++++++++++--------------
 assets/email_template.html            | 39 +++----------
 docs/README.md                        |  8 +--
 lib/NfcoreSchema.groovy               |  4 +-
 9 files changed, 74 insertions(+), 111 deletions(-)
 delete mode 100644 .markdownlint.yml

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 9807f30c..9ae9e986 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -15,8 +15,7 @@ Contributions to the code are even more welcome ;)
 
 If you'd like to write some code for nf-core/rnafusion, the standard workflow is as follows:
 
-1. Check that there isn't already an issue about your idea in the [nf-core/rnafusion issues](https://github.com/nf-core/rnafusion/issues) to avoid duplicating work
-    - If there isn't one already, please create one so that others know you're working on this
+1. Check that there isn't already an issue about your idea in the [nf-core/rnafusion issues](https://github.com/nf-core/rnafusion/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this
 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rnafusion repository](https://github.com/nf-core/rnafusion) to your GitHub account
 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
 4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
@@ -49,9 +48,9 @@ These tests are run both with the latest available version of `Nextflow` and als
 
 :warning: Only in the unlikely and regretful event of a release happening with a bug.
 
--   On your own fork, make a new branch `patch` based on `upstream/master`.
--   Fix the bug, and bump version (X.Y.Z+1).
--   A PR should be made on `master` from patch to directly this particular bug.
+- On your own fork, make a new branch `patch` based on `upstream/master`.
+- Fix the bug, and bump version (X.Y.Z+1).
+- A PR should be made on `master` from patch to directly this particular bug.
 
 ## Getting help
 
@@ -73,7 +72,7 @@ If you wish to contribute a new step, please use the following coding standards:
 6. Add sanity checks and validation for all relevant parameters.
 7. Perform local tests to validate that the new code works as expected.
 8. If applicable, add a new test command in `.github/workflow/ci.yml`.
-9. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module.
+9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module.
 10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`.
 
 ### Default values
@@ -92,8 +91,8 @@ The process resources can be passed on to the tool dynamically within the proces
 
 Please use the following naming schemes, to make it easy to understand what is going where.
 
--   initial process channel: `ch_output_from_<process>`
--   intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
+- initial process channel: `ch_output_from_<process>`
+- intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
 
 ### Nextflow version bumping
 
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index bca6064b..bc52a0a2 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -10,17 +10,16 @@ Remember that PRs should be made against the dev branch, unless you're preparing
 
 Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
 -->
-<!-- markdownlint-disable ul-indent -->
 
 ## PR checklist
 
--   [ ] This comment contains a description of changes (with reason).
--   [ ] If you've fixed a bug or added code that should be tested, add tests!
-    -   [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
-    -   [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
--   [ ] Make sure your code lints (`nf-core lint`).
--   [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
--   [ ] Usage Documentation in `docs/usage.md` is updated.
--   [ ] Output Documentation in `docs/output.md` is updated.
--   [ ] `CHANGELOG.md` is updated.
--   [ ] `README.md` is updated (including new tool citations and authors/contributors).
+- [ ] This comment contains a description of changes (with reason).
+- [ ] If you've fixed a bug or added code that should be tested, add tests!
+  - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
+  - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
+- [ ] Make sure your code lints (`nf-core lint`).
+- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
+- [ ] Usage Documentation in `docs/usage.md` is updated.
+- [ ] Output Documentation in `docs/output.md` is updated.
+- [ ] `CHANGELOG.md` is updated.
+- [ ] `README.md` is updated (including new tool citations and authors/contributors).
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index ccb7211b..8623dfc2 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -13,7 +13,7 @@ jobs:
       - name: Check PRs
         if: github.repository == 'nf-core/rnafusion'
         run: |
-          { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
+          "{ [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]"
 
       # If the above check failed, post a comment on the PR explaining the failure
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
@@ -42,3 +42,4 @@ jobs:
             Thanks again for your contribution!
           repo-token: ${{ secrets.GITHUB_TOKEN }}
           allow-repeats: false
+#
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 04758f61..91c487a1 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -26,3 +26,4 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
           path: linting-logs/lint_results.md
+#
diff --git a/.markdownlint.yml b/.markdownlint.yml
deleted file mode 100644
index 779ba6c0..00000000
--- a/.markdownlint.yml
+++ /dev/null
@@ -1,14 +0,0 @@
-# Markdownlint configuration file
-default: true
-line-length: false
-ul-indent:
-  indent: 4
-no-duplicate-header:
-  siblings_only: true
-no-inline-html:
-  allowed_elements:
-    - img
-    - p
-    - kbd
-    - details
-    - summary
diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md
index a47b923c..f4fd052f 100644
--- a/CODE_OF_CONDUCT.md
+++ b/CODE_OF_CONDUCT.md
@@ -4,19 +4,19 @@
 
 In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of:
 
--   Age
--   Body size
--   Familial status
--   Gender identity and expression
--   Geographical location
--   Level of experience
--   Nationality and national origins
--   Native language
--   Physical and neurological ability
--   Race or ethnicity
--   Religion
--   Sexual identity and orientation
--   Socioeconomic status
+- Age
+- Body size
+- Familial status
+- Gender identity and expression
+- Geographical location
+- Level of experience
+- Nationality and national origins
+- Native language
+- Physical and neurological ability
+- Race or ethnicity
+- Religion
+- Sexual identity and orientation
+- Socioeconomic status
 
 Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance.
 
@@ -48,38 +48,38 @@ Members of the core team or the safety officer who violate the CoC will be requi
 
 Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference:
 
--   Communicating with an official project email address.
--   Communicating with community members within the nf-core Slack channel.
--   Participating in hackathons organised by nf-core (both online and in-person events).
--   Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence.
--   Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc.
--   Representing nf-core on social media. This includes both official and personal accounts.
+- Communicating with an official project email address.
+- Communicating with community members within the nf-core Slack channel.
+- Participating in hackathons organised by nf-core (both online and in-person events).
+- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence.
+- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc.
+- Representing nf-core on social media. This includes both official and personal accounts.
 
 ## nf-core cares 😊
 
 nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order):
 
--   Ask for consent before sharing another community member’s personal information (including photographs) on social media.
--   Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity.
--   Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !)
--   Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.)
--   Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can)
--   Focus on what is best for the team and the community. (When in doubt, ask)
--   Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn.
--   Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!)
--   Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**)
--   Take breaks when you feel like you need them.
--   Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.)
+- Ask for consent before sharing another community member’s personal information (including photographs) on social media.
+- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity.
+- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !)
+- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.)
+- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can)
+- Focus on what is best for the team and the community. (When in doubt, ask)
+- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn.
+- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!)
+- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**)
+- Take breaks when you feel like you need them.
+- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.)
 
 ## nf-core frowns on 😕
 
 The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces.
 
--   Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom.
--   “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online.
--   Spamming or trolling of individuals on social media.
--   Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention.
--   Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience.
+- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom.
+- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online.
+- Spamming or trolling of individuals on social media.
+- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention.
+- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience.
 
 ### Online Trolling
 
@@ -99,13 +99,13 @@ All reports will be handled with utmost discretion and confidentially.
 
 ## Attribution and Acknowledgements
 
--   The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4)
--   The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition)
--   The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/)
--   The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla)
+- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4)
+- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition)
+- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/)
+- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla)
 
 ## Changelog
 
 ### v1.0 - March 12th, 2021
 
--   Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC.
+- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC.
diff --git a/assets/email_template.html b/assets/email_template.html
index 82aea34e..306f8e4f 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -4,21 +4,12 @@
     <meta http-equiv="X-UA-Compatible" content="IE=edge" />
     <meta name="viewport" content="width=device-width, initial-scale=1" />
 
-    <meta
-      name="description"
-      content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community."
-    />
+    <!-- prettier-ignore -->
+    <meta name="description" content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community." />
     <title>nf-core/rnafusion Pipeline Report</title>
   </head>
   <body>
-    <div
-      style="
-        font-family: Helvetica, Arial, sans-serif;
-        padding: 30px;
-        max-width: 800px;
-        margin: 0 auto;
-      "
-    >
+    <div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto">
       <img src="cid:nfcorepipelinelogo" />
 
       <h1>nf-core/rnafusion v${version}</h1>
@@ -36,17 +27,10 @@ <h2>Run Name: $runName</h2>
           border-radius: 4px;
         "
       >
-        <h4 style="margin-top: 0; color: inherit">
-          nf-core/rnafusion execution completed unsuccessfully!
-        </h4>
-        <p>
-          The exit status of the task that caused the workflow execution to fail
-          was: <code>$exitStatus</code>.
-        </p>
+        <h4 style="margin-top: 0; color: inherit">nf-core/rnafusion execution completed unsuccessfully!</h4>
+        <p>The exit status of the task that caused the workflow execution to fail was: <code>$exitStatus</code>.</p>
         <p>The full error message was:</p>
-        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0">
-${errorReport}</pre
-        >
+        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0">${errorReport}</pre>
       </div>
       """ } else { out << """
       <div
@@ -64,10 +48,7 @@ <h4 style="margin-top: 0; color: inherit">
       </div>
       """ } %>
 
-      <p>
-        The workflow was completed at <strong>$dateComplete</strong> (duration:
-        <strong>$duration</strong>)
-      </p>
+      <p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
       <p>The command used to launch the workflow was as follows:</p>
       <pre
         style="
@@ -124,11 +105,7 @@ <h3>Pipeline Configuration:</h3>
       </table>
 
       <p>nf-core/rnafusion</p>
-      <p>
-        <a href="https://github.com/nf-core/rnafusion"
-          >https://github.com/nf-core/rnafusion</a
-        >
-      </p>
+      <p><a href="https://github.com/nf-core/rnafusion">https://github.com/nf-core/rnafusion</a></p>
     </div>
   </body>
 </html>
diff --git a/docs/README.md b/docs/README.md
index f8520ea5..0c1d417d 100644
--- a/docs/README.md
+++ b/docs/README.md
@@ -2,9 +2,9 @@
 
 The nf-core/rnafusion documentation is split into the following pages:
 
--   [Usage](usage.md)
-    -   An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
--   [Output](output.md)
-    -   An overview of the different results produced by the pipeline and how to interpret them.
+- [Usage](usage.md)
+  - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
+- [Output](output.md)
+  - An overview of the different results produced by the pipeline and how to interpret them.
 
 You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re)
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index 40ab65f2..b3d092f8 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -27,7 +27,7 @@ class NfcoreSchema {
     /* groovylint-disable-next-line UnusedPrivateMethodParameter */
     public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') {
         def has_error = false
-        //=====================================================================//
+        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
         // Check for nextflow core params and unexpected params
         def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text
         def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
@@ -135,7 +135,7 @@ class NfcoreSchema {
             }
         }
 
-        //=====================================================================//
+        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
         // Validate parameters against the schema
         InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream()
         JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream))

From 43922e0dc28d712c16f30bfda64e52120d23f8ac Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Mar 2022 14:38:13 +0100
Subject: [PATCH 234/359] prettier

---
 CHANGELOG.md   | 150 ++++++++++++++++++++---------------------
 CITATIONS.md   |  39 +++++------
 README.md      |  32 ++++-----
 docs/output.md | 178 ++++++++++++++++++++++++-------------------------
 docs/usage.md  |  21 +++---
 5 files changed, 210 insertions(+), 210 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 098c63ab..43a81358 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -9,41 +9,41 @@ Update to DSL2 and newer software/reference versions
 
 ### Non-DSL2 related changes
 
--   replace gtf file with chr.gtf: Please confirm if this change is ok for the whole pipeline
--   remove subdirectory with params.genome as only GRCh38 is supported (not necessary but helped me see the output more clearly, can be reversed)
--   modification of output parameters to make sure every important file is published
--   additional build_references flag to trigger the workflow to build references
--   container for starfusion is changed and the absolute path to prep_genome_lib.pl is given
-    --fasta argument is not required when executing the workflow to build references
--   increase cpu + memory for process STARFUSION_DOWNLOAD
--   ci test done on stubs of reference building subprocesses ensembl and arriba
--   **For the moment, the rnafusion workflow (to analyse data and not build the references) is removed as dependencies and links would have to be fixed in the rnafusion workflow for execution of the build references otherwise**
+- replace gtf file with chr.gtf: Please confirm if this change is ok for the whole pipeline
+- remove subdirectory with params.genome as only GRCh38 is supported (not necessary but helped me see the output more clearly, can be reversed)
+- modification of output parameters to make sure every important file is published
+- additional build_references flag to trigger the workflow to build references
+- container for starfusion is changed and the absolute path to prep_genome_lib.pl is given
+  --fasta argument is not required when executing the workflow to build references
+- increase cpu + memory for process STARFUSION_DOWNLOAD
+- ci test done on stubs of reference building subprocesses ensembl and arriba
+- **For the moment, the rnafusion workflow (to analyse data and not build the references) is removed as dependencies and links would have to be fixed in the rnafusion workflow for execution of the build references otherwise**
 
 ---
 
 ### Added
 
--   Added `qualimap/rnaseq v2.2.2d` from nf-core modules
--   Added UCSC `gtfToGenePred v377`
--   Added `picard CollectRnaSeqMetrics v2.26.10`
--   Added `picard MarkDuplicates v2.26.10` from nf-core modules
--   Added `cat/fastqc` from nf-core modules
--   Added possibility for manually feeding the results of fusions from different tools to speed-up reruns
+- Added `qualimap/rnaseq v2.2.2d` from nf-core modules
+- Added UCSC `gtfToGenePred v377`
+- Added `picard CollectRnaSeqMetrics v2.26.10`
+- Added `picard MarkDuplicates v2.26.10` from nf-core modules
+- Added `cat/fastqc` from nf-core modules
+- Added possibility for manually feeding the results of fusions from different tools to speed-up reruns
 
 ### Changed
 
--   Upgrade to `nf-core/tools v2.3`
--   Upgrade `Arriba v1.2.0` to `Arriba v2.1.0`
--   Upgrade `FusionCatcher v1.20` to `FusionCatcher v1.33`
--   Upgrade `STAR-fusion v1.8.1` to `STAR-fusion v1.10.1`
--   Upgrade `STAR v2.7.1` to `STAR v2.7.9`
--   Upgrade `fusion-report v2.1.3` to `fusion-report v2.1.5`
--   Upgrade `kallisto v0.44.0` to `kallisto v0.46.2`
--   Upgrade `fastqc v0.11.8` to `fastqc v0.11.9`
--   Upgrade `samtools v1.9` to `samtools v1.15`
--   Upgrade `arriba` references from `v1.2.0` to `v2.1.0`
--   Upgrade `fusioncatcher` references from `v98` to `v102`
--   Use `arriba` (detect only), `kallisto` and `STAR` from nf-core modules
+- Upgrade to `nf-core/tools v2.3`
+- Upgrade `Arriba v1.2.0` to `Arriba v2.1.0`
+- Upgrade `FusionCatcher v1.20` to `FusionCatcher v1.33`
+- Upgrade `STAR-fusion v1.8.1` to `STAR-fusion v1.10.1`
+- Upgrade `STAR v2.7.1` to `STAR v2.7.9`
+- Upgrade `fusion-report v2.1.3` to `fusion-report v2.1.5`
+- Upgrade `kallisto v0.44.0` to `kallisto v0.46.2`
+- Upgrade `fastqc v0.11.8` to `fastqc v0.11.9`
+- Upgrade `samtools v1.9` to `samtools v1.15`
+- Upgrade `arriba` references from `v1.2.0` to `v2.1.0`
+- Upgrade `fusioncatcher` references from `v98` to `v102`
+- Use `arriba` (detect only), `kallisto` and `STAR` from nf-core modules
 
 Parameters for `STAR` for `arriba` changed from:
 
@@ -150,86 +150,86 @@ to
 
 ### Removed
 
--   Ericscript tool
--   GRCh37 support
--   Running with conda
+- Ericscript tool
+- GRCh37 support
+- Running with conda
 
 ## v1.3.0dev nfcore/rnafusion - 2020/07/15
 
--   Using official STAR-Fusion container [#160](https://github.com/nf-core/rnafusion/issues/160)
+- Using official STAR-Fusion container [#160](https://github.com/nf-core/rnafusion/issues/160)
 
 ### Added
 
--   Added social preview image [#107](https://github.com/nf-core/rnafusion/issues/107)
--   Added support for GRCh37 genome assembly [#77](https://github.com/nf-core/rnafusion/issues/77)
+- Added social preview image [#107](https://github.com/nf-core/rnafusion/issues/107)
+- Added support for GRCh37 genome assembly [#77](https://github.com/nf-core/rnafusion/issues/77)
 
 ### Changed
 
--   Upgrade `fusion-report v2.1.2` to `fusion-report v2.1.3`
--   Upgrade `fusion-report v2.1.1` to `fusion-report v2.1.2`
--   Upgrade `fusion-report v2.1.0` to `fusion-report v2.1.1`
--   Upgrade `Arriba v1.1.0` to `Arriba v1.2.0`
--   Upgrade `fusion-report v2.0.2` to `fusion-report v2.1.0`
+- Upgrade `fusion-report v2.1.2` to `fusion-report v2.1.3`
+- Upgrade `fusion-report v2.1.1` to `fusion-report v2.1.2`
+- Upgrade `fusion-report v2.1.0` to `fusion-report v2.1.1`
+- Upgrade `Arriba v1.1.0` to `Arriba v1.2.0`
+- Upgrade `fusion-report v2.0.2` to `fusion-report v2.1.0`
 
 ### Fixed
 
--   Missing `strip-components` in `download-references.nf/star-fusion` [#148](https://github.com/nf-core/rnafusion/issues/148)
--   Missing version prefix for cdna [#143](https://github.com/nf-core/rnafusion/issues/143)
--   `samtools` missing header in empty file for FusionInspector [ref](https://github.com/STAR-Fusion/STAR-Fusion/issues/191)
--   Removed `profile` from helper scripts [#139](https://github.com/nf-core/rnafusion/issues/139)
--   Wrong url path for `Pfam-A.hmm.gz` [#140](https://github.com/nf-core/rnafusion/issues/140)
+- Missing `strip-components` in `download-references.nf/star-fusion` [#148](https://github.com/nf-core/rnafusion/issues/148)
+- Missing version prefix for cdna [#143](https://github.com/nf-core/rnafusion/issues/143)
+- `samtools` missing header in empty file for FusionInspector [ref](https://github.com/STAR-Fusion/STAR-Fusion/issues/191)
+- Removed `profile` from helper scripts [#139](https://github.com/nf-core/rnafusion/issues/139)
+- Wrong url path for `Pfam-A.hmm.gz` [#140](https://github.com/nf-core/rnafusion/issues/140)
 
 ### Removed
 
--   Removed `scripts/download-singularity-img.sh` and `download-singularity-img.nf` as they are not necessary any more
+- Removed `scripts/download-singularity-img.sh` and `download-singularity-img.nf` as they are not necessary any more
 
 ---
 
 ## [1.1.0] nfcore/rnafusion - 2020/02/10
 
--   Fusion gene detection tools:
-    -   `Arriba v1.1.0`
-    -   `Ericscript v0.5.5`
-    -   `Fusioncatcher v1.20`
-    -   `Pizzly v0.37.3`
-    -   `Squid v1.5`
-    -   `STAR-Fusion v1.6.0`
--   Visualization tools:
-    -   `Arriba v1.1.0`
-    -   `FusionInspector v1.3.1`
--   Other tools:
-    -   `fusion-report v2.0.1`
-    -   `FastQ v0.11.8`
-    -   `MultiQC v1.7`
-    -   `STAR aligner v2.7.0f`
+- Fusion gene detection tools:
+  - `Arriba v1.1.0`
+  - `Ericscript v0.5.5`
+  - `Fusioncatcher v1.20`
+  - `Pizzly v0.37.3`
+  - `Squid v1.5`
+  - `STAR-Fusion v1.6.0`
+- Visualization tools:
+  - `Arriba v1.1.0`
+  - `FusionInspector v1.3.1`
+- Other tools:
+  - `fusion-report v2.0.1`
+  - `FastQ v0.11.8`
+  - `MultiQC v1.7`
+  - `STAR aligner v2.7.0f`
 
 ### Added
 
--   Added `Arriba 1.1.0` [#63](https://github.com/nf-core/rnafusion/issues/63)
--   Added Batch mode [#54](https://github.com/nf-core/rnafusion/issues/54)
+- Added `Arriba 1.1.0` [#63](https://github.com/nf-core/rnafusion/issues/63)
+- Added Batch mode [#54](https://github.com/nf-core/rnafusion/issues/54)
 
 ### Changed
 
--   Updated examples and configurations
--   Upgraded `fusion-report v1.0.0` to `fusion-report v2.0.1`
--   Divided `running_tools` into fusion and visualization tools
--   Updated `STAR` in `Squid`, `Fusion-Inspector` version to `2.7.0f`
--   Upgraded `STAR-Fusion v1.5.0` to `STAR-Fusion v1.6.0` [#83](https://github.com/nf-core/rnafusion/issues/83)
--   Parameter `igenomesIgnore` renamed to `igenome` [#81](https://github.com/nf-core/rnafusion/issues/81)
--   Finished STAR-Fusion file renaming [#18](https://github.com/nf-core/rnafusion/issues/18)
--   Updated logos
--   Updated to nf-core `1.8` TEMPLATE
+- Updated examples and configurations
+- Upgraded `fusion-report v1.0.0` to `fusion-report v2.0.1`
+- Divided `running_tools` into fusion and visualization tools
+- Updated `STAR` in `Squid`, `Fusion-Inspector` version to `2.7.0f`
+- Upgraded `STAR-Fusion v1.5.0` to `STAR-Fusion v1.6.0` [#83](https://github.com/nf-core/rnafusion/issues/83)
+- Parameter `igenomesIgnore` renamed to `igenome` [#81](https://github.com/nf-core/rnafusion/issues/81)
+- Finished STAR-Fusion file renaming [#18](https://github.com/nf-core/rnafusion/issues/18)
+- Updated logos
+- Updated to nf-core `1.8` TEMPLATE
 
 ### Fixed
 
--   iGenomes optional, but not really [#91](https://github.com/nf-core/rnafusion/issues/91)
--   Updated `fusioncatcher` to latest `1.20` version also solving [#95](https://github.com/nf-core/rnafusion/issues/95)
+- iGenomes optional, but not really [#91](https://github.com/nf-core/rnafusion/issues/91)
+- Updated `fusioncatcher` to latest `1.20` version also solving [#95](https://github.com/nf-core/rnafusion/issues/95)
 
 ### Removed
 
--   Variables `pizzly_fasta` and `pizzly_gtf` have been removed and replaced with `transcript` and `gtf`
--   `Jenkisfile`, test configuration, pylintrc configuration
--   Removed `igenomes.config` because the pipeline only supports `Ensembl` version
+- Variables `pizzly_fasta` and `pizzly_gtf` have been removed and replaced with `transcript` and `gtf`
+- `Jenkisfile`, test configuration, pylintrc configuration
+- Removed `igenomes.config` because the pipeline only supports `Ensembl` version
 
 ---
 
diff --git a/CITATIONS.md b/CITATIONS.md
index 94282261..629f86ad 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -13,42 +13,43 @@
 - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
 
 - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
+
   > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
 
--   [Arriba](https://github.com/suhrig/arriba)
+- [Arriba](https://github.com/suhrig/arriba)
 
-    > Uhrig S, Ellermann J, Walther T, Burkhardt P, Fröhlich M, Hutter B, Toprak UH, Neumann O, Stenzinger A, Scholl C, Fröhling S, Brors B. Accurate and efficient detection of gene fusions from RNA sequencing data.
-    > Genome Research. 2021 Mar 31;448-460. doi: 10.1101/gr.257246.119. Epub 2021 Jan 13. PubMed PMID: 33441414; PubMed Central PMCID: PMC7919457.
+  > Uhrig S, Ellermann J, Walther T, Burkhardt P, Fröhlich M, Hutter B, Toprak UH, Neumann O, Stenzinger A, Scholl C, Fröhling S, Brors B. Accurate and efficient detection of gene fusions from RNA sequencing data.
+  > Genome Research. 2021 Mar 31;448-460. doi: 10.1101/gr.257246.119. Epub 2021 Jan 13. PubMed PMID: 33441414; PubMed Central PMCID: PMC7919457.
 
--   [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
+- [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
 
-    > Nicorici D, Satalan M, Edgren H, Kangaspeska S, Murumagi A, Kallioniemi O, Virtanen S, Kilkku O. FusionCatcher – a tool for finding somatic fusion genes in paired-end RNA-sequencing data. BioRxiv, 2014 Nov. doi: 10.1101/011650.
+  > Nicorici D, Satalan M, Edgren H, Kangaspeska S, Murumagi A, Kallioniemi O, Virtanen S, Kilkku O. FusionCatcher – a tool for finding somatic fusion genes in paired-end RNA-sequencing data. BioRxiv, 2014 Nov. doi: 10.1101/011650.
 
--   [Fusion-report](https://github.com/matq007/fusion-report)
+- [Fusion-report](https://github.com/matq007/fusion-report)
 
-    > Proks M, Genomic Profiling of a Comprehensive Nation-wide Collection of Childhood Solid Tumors, Master Thesis, Supervisors: Grøntved L, Díaz de Ståhl T, Nistér M, Ewels P, Garcia MU, Juhos S, University of Southern Denmark, 2019, unpublished.
+  > Proks M, Genomic Profiling of a Comprehensive Nation-wide Collection of Childhood Solid Tumors, Master Thesis, Supervisors: Grøntved L, Díaz de Ståhl T, Nistér M, Ewels P, Garcia MU, Juhos S, University of Southern Denmark, 2019, unpublished.
 
--   [Kallisto](https://pachterlab.github.io/kallisto/)
+- [Kallisto](https://pachterlab.github.io/kallisto/)
 
-    > Bray NL, Pimentel H, Melsted P, Pachter L. Near-optimal probabilistic RNA-seq quantification. Nature Biotechnology 2016 Apr. 34, 525–527. doi:10.1038/nbt.3519. PMID: 27043002.
+  > Bray NL, Pimentel H, Melsted P, Pachter L. Near-optimal probabilistic RNA-seq quantification. Nature Biotechnology 2016 Apr. 34, 525–527. doi:10.1038/nbt.3519. PMID: 27043002.
 
--   [Pizzly](https://github.com/pmelsted/pizzly)
-    Melsted P, Hateley S, Joseph IC, Pimentel H, Bray N, Pachter L. Fusion detection and quantification by pseudoalignment. BioRxiv, 2017 Jul. doi: 10.1101/166322.
+- [Pizzly](https://github.com/pmelsted/pizzly)
+  Melsted P, Hateley S, Joseph IC, Pimentel H, Bray N, Pachter L. Fusion detection and quantification by pseudoalignment. BioRxiv, 2017 Jul. doi: 10.1101/166322.
 
--   [SAMtools](https://pubmed.ncbi.nlm.nih.gov/19505943/)
+- [SAMtools](https://pubmed.ncbi.nlm.nih.gov/19505943/)
 
-    > Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PubMed PMID: 19505943; PubMed Central PMCID: PMC2723002.
+  > Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PubMed PMID: 19505943; PubMed Central PMCID: PMC2723002.
 
--   [Squid](https://github.com/Kingsford-Group/squid)
+- [Squid](https://github.com/Kingsford-Group/squid)
 
-    > Ma C, Shao M, Kingsford C. SQUID: transcriptomic structural variation detection from RNA-seq. Genome Biol 2028 Apr. 19, 52. doi: 10.1186/s13059-018-1421-5. PubMed PMID: 29650026. PubMed Central PMCID: PMC5896115.
+  > Ma C, Shao M, Kingsford C. SQUID: transcriptomic structural variation detection from RNA-seq. Genome Biol 2028 Apr. 19, 52. doi: 10.1186/s13059-018-1421-5. PubMed PMID: 29650026. PubMed Central PMCID: PMC5896115.
 
--   [STAR](https://pubmed.ncbi.nlm.nih.gov/23104886/)
+- [STAR](https://pubmed.ncbi.nlm.nih.gov/23104886/)
 
-    > Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner Bioinformatics. 2013 Jan 1;29(1):15-21. doi: 10.1093/bioinformatics/bts635. Epub 2012 Oct 25. PubMed PMID: 23104886; PubMed Central PMCID: PMC3530905.
+  > Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner Bioinformatics. 2013 Jan 1;29(1):15-21. doi: 10.1093/bioinformatics/bts635. Epub 2012 Oct 25. PubMed PMID: 23104886; PubMed Central PMCID: PMC3530905.
 
--   [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)
-    > Haas BJ, Dobin A, Li B, Stransky N, Pochet N, Regev A. Accuracy assessment of fusion transcript detection via read-mapping and de novo fusion transcript assembly-based methods. Genome Biology 2019 Oct;20,213. doi: 10.1186/s13059-019-1842-9
+- [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)
+  > Haas BJ, Dobin A, Li B, Stransky N, Pochet N, Regev A. Accuracy assessment of fusion transcript detection via read-mapping and de novo fusion transcript assembly-based methods. Genome Biology 2019 Oct;20,213. doi: 10.1186/s13059-019-1842-9
 
 ## Software packaging/containerisation tools
 
diff --git a/README.md b/README.md
index 8bb1fe22..16c62a3d 100644
--- a/README.md
+++ b/README.md
@@ -34,6 +34,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 | [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) | :white_check_mark: | `1.10.1` |
 
 <!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
+
 On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).
 
 ## Pipeline summary
@@ -57,25 +58,25 @@ On release, automated continuous integration tests run the pipeline on a full-si
 3. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
 4. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 5. Arriba subworkflow
-    - STAR alignment
-    - Samtool sort
-    - Samtool index
-    - [Arriba](https://github.com/suhrig/arriba) fusion detection
-    - [Arriba](https://github.com/suhrig/arriba) visualisation
+   - STAR alignment
+   - Samtool sort
+   - Samtool index
+   - [Arriba](https://github.com/suhrig/arriba) fusion detection
+   - [Arriba](https://github.com/suhrig/arriba) visualisation
 6. Pizzly subworkflow
-    - [Kallisto](https://pachterlab.github.io/kallisto/) quantification
-    - [Pizzly](https://github.com/pmelsted/pizzly) fusion detection
+   - [Kallisto](https://pachterlab.github.io/kallisto/) quantification
+   - [Pizzly](https://github.com/pmelsted/pizzly) fusion detection
 7. Squid subworkflow
-    - [STAR](https://github.com/alexdobin/STAR) alignment
-    - [Samtools view](http://www.htslib.org/): convert sam output from STAR to bam
-    - [Samtools sort](http://www.htslib.org/): bam output from STAR
-    - [Squid](https://github.com/Kingsford-Group/squid) fusion detection
-    - [Squid](https://github.com/Kingsford-Group/squid) annotate
+   - [STAR](https://github.com/alexdobin/STAR) alignment
+   - [Samtools view](http://www.htslib.org/): convert sam output from STAR to bam
+   - [Samtools sort](http://www.htslib.org/): bam output from STAR
+   - [Squid](https://github.com/Kingsford-Group/squid) fusion detection
+   - [Squid](https://github.com/Kingsford-Group/squid) annotate
 8. STAR-fusion subworkflow
-    - [STAR](https://github.com/alexdobin/STAR) alignment
-    - [STAR-fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
+   - [STAR](https://github.com/alexdobin/STAR) alignment
+   - [STAR-fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
 9. Fusioncatcher subworkflow
-    - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
+   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
 10. Fusion-report subworkflow
     - Merge all fusions detected by the different tools
     - [Fusion-report](https://github.com/matq007/fusion-report)
@@ -123,7 +124,6 @@ We thank the following people for their extensive assistance in the development
 
 - Annick Renevey (@rannick)
 
-
 ## Contributions and Support
 
 If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).
diff --git a/docs/output.md b/docs/output.md
index 68299aa4..69bc1387 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -10,53 +10,53 @@ The directories listed below will be created in the results directory after the
 
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
--   [Download and build references](#references) - Build references needed to run the rest of the pipeline
--   [STAR](#star) - Alignment for arriba, squid and STAR-fusion
--   [Cat](#cat) - Concatenated fastq files per sample ID
--   [Arriba](#arriba) - Arriba fusion detection
--   [Pizzly](#pizzly) - Pizzly fusion detection
--   [Squid](#squid) - Squid fusion detection
--   [STAR-fusion](#starfusion) - STAR-fusion fusion detection
--   [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
--   [Samtools](#samtools) - SAM/BAM file manipulation
--   [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report
--   [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman and FusionGBD databases
--   [FusionInspector](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
--   [Qualimap](#qualimap) - quality control of alignment
--   [Picard](#picard) - collect metrics
--   [FastQC](#fastqc) - Raw read quality control
--   [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
--   [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
+- [Download and build references](#references) - Build references needed to run the rest of the pipeline
+- [STAR](#star) - Alignment for arriba, squid and STAR-fusion
+- [Cat](#cat) - Concatenated fastq files per sample ID
+- [Arriba](#arriba) - Arriba fusion detection
+- [Pizzly](#pizzly) - Pizzly fusion detection
+- [Squid](#squid) - Squid fusion detection
+- [STAR-fusion](#starfusion) - STAR-fusion fusion detection
+- [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
+- [Samtools](#samtools) - SAM/BAM file manipulation
+- [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report
+- [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman and FusionGBD databases
+- [FusionInspector](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
+- [Qualimap](#qualimap) - quality control of alignment
+- [Picard](#picard) - collect metrics
+- [FastQC](#fastqc) - Raw read quality control
+- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
+- [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ### Download and build references
 
 <details markdown="1">
 <summary>Output files</summary>
 
--   `genomes_base/`
-    -   `arriba`
-        -   `blacklist_hg38_GRCh38_v2.1.0.tsv.gz`
-        -   `protein_domains_hg38_GRCh38_v2.1.0.gff3`
-        -   `cytobands_hg38_GRCh38_v2.1.0.tsv`
-    -   `ensembl`
-        -   `Homo_sapiens.GRCh38.{ensembl_version}.all.fa`
-        -   `Homo_sapiens.GRCh38.{ensembl_version}.cdna.all.fa.gz`
-        -   `Homo_sapiens.GRCh38.{ensembl_version}.gtf`
-        -   `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf`
-        -   `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf.refflat`
-    -   `fusioncatcher`
-        -   `human_v<version>` - dir with all references for fusioncatcher
-    -   `fusion_report_db`
-        -   `cosmic.db`
-        -   `fusiongdb.db`
-        -   `fusiongdb2.db`
-        -   `mitelman.db`
-    -   `pizzly`
-        -   `kallisto` - file containing the kallisto index
-    -   `star` - dir with STAR index
-    -   `starfusion`
-        -   files and dirs used to build the index
-        -   `ctat_genome_lib_build_dir` - dir containing the index
+- `genomes_base/`
+  - `arriba`
+    - `blacklist_hg38_GRCh38_v2.1.0.tsv.gz`
+    - `protein_domains_hg38_GRCh38_v2.1.0.gff3`
+    - `cytobands_hg38_GRCh38_v2.1.0.tsv`
+  - `ensembl`
+    - `Homo_sapiens.GRCh38.{ensembl_version}.all.fa`
+    - `Homo_sapiens.GRCh38.{ensembl_version}.cdna.all.fa.gz`
+    - `Homo_sapiens.GRCh38.{ensembl_version}.gtf`
+    - `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf`
+    - `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf.refflat`
+  - `fusioncatcher`
+    - `human_v<version>` - dir with all references for fusioncatcher
+  - `fusion_report_db`
+    - `cosmic.db`
+    - `fusiongdb.db`
+    - `fusiongdb2.db`
+    - `mitelman.db`
+  - `pizzly`
+    - `kallisto` - file containing the kallisto index
+  - `star` - dir with STAR index
+  - `starfusion`
+    - files and dirs used to build the index
+    - `ctat_genome_lib_build_dir` - dir containing the index
 
 (Only files or folders used by the pipeline are mentioned explicitly.)
 
@@ -135,7 +135,7 @@ For STAR-fusion with the parameters:
 <details markdown="1">
 <summary>Output files</summary>
 
--   `star_for_<tool>`
+- `star_for_<tool>`
 _ **Common**
 _ `<sample_id>.Log.final.out`
 _ `<sample_id>.Log.progress.out`
@@ -159,9 +159,9 @@ Cat is used to concatenate fastq files belonging to the same sample.
 <details markdown="1">
 <summary>Output files</summary>
 
--   `cat`
-    -   `<sample_id>_1.merged.fastq.gz`
-    -   `<sample_id>_2.merged.fastq.gz`
+- `cat`
+  - `<sample_id>_1.merged.fastq.gz`
+  - `<sample_id>_2.merged.fastq.gz`
 
 </details>
 
@@ -174,9 +174,9 @@ Arriba is used for i) detect fusion and ii) output a PDF report for the fusions
 <details markdown="1">
 <summary>Output files</summary>
 
--   `arriba`
-    -   `<sample_id>.arriba.fusions.tsv` - contains the identified fusions
-    -   `<sample_id>.arriba.fusions.discarded.tsv`
+- `arriba`
+  - `<sample_id>.arriba.fusions.tsv` - contains the identified fusions
+  - `<sample_id>.arriba.fusions.discarded.tsv`
 
 </details>
 
@@ -185,8 +185,8 @@ Arriba is used for i) detect fusion and ii) output a PDF report for the fusions
 <details markdown="1">
 <summary>Output files</summary>
 
--   `arriba_visualisation`
-    -   `<sample_id>.pdf`
+- `arriba_visualisation`
+  - `<sample_id>.pdf`
 
 </details>
 
@@ -199,8 +199,8 @@ The first step of the pizzly workflow is to run `kallisto quant`:
 <details markdown="1">
 <summary>Output files</summary>
 
--   `kallisto`
-    -   `<sample_id>.kallisto_quant.fusions.txt`
+- `kallisto`
+  - `<sample_id>.kallisto_quant.fusions.txt`
 
 </details>
 
@@ -220,9 +220,9 @@ Pizzly uses the following arguments:
 <details markdown="1">
 <summary>Output files</summary>
 
--   `pizzly`
-    -   `<sample_id>.pizzly.txt` - contains the identified fusions
-    -   `<sample_id>.pizzly.unfiltered.json`
+- `pizzly`
+  - `<sample_id>.pizzly.txt` - contains the identified fusions
+  - `<sample_id>.pizzly.unfiltered.json`
 
 </details>
 
@@ -233,9 +233,9 @@ Squid is run in two steps: i) fusion detection and ii) fusion annotation but the
 <details markdown="1">
 <summary>Output files</summary>
 
--   `squid`
-    -   `<sample_id>.squid.fusions_sv.txt` - contains the identified fusions
-    -   `<sample_id>.squid.fusions.annotated.txt`- contains the identified fusions annotatedvi
+- `squid`
+  - `<sample_id>.squid.fusions_sv.txt` - contains the identified fusions
+  - `<sample_id>.squid.fusions.annotated.txt`- contains the identified fusions annotatedvi
 
 </details>
 
@@ -244,10 +244,10 @@ Squid is run in two steps: i) fusion detection and ii) fusion annotation but the
 <details markdown="1">
 <summary>Output files</summary>
 
--   `starfusion`
-    -   `<sample_id>.starfusion.fusion_predictions.tsv` - contains the identified fusions
-    -   `<sample_id>.starfusion.abridged.tsv`
-    -   `- contains the identified fusions.starfusion.abridged.coding_effect.tsv`
+- `starfusion`
+  - `<sample_id>.starfusion.fusion_predictions.tsv` - contains the identified fusions
+  - `<sample_id>.starfusion.abridged.tsv`
+  - `- contains the identified fusions.starfusion.abridged.coding_effect.tsv`
 
 </details>
 
@@ -256,7 +256,7 @@ Squid is run in two steps: i) fusion detection and ii) fusion annotation but the
 <details markdown="1">
 <summary>Output files</summary>
 
--   `fusioncatcher`
+- `fusioncatcher`
 _ `<sample_id>.fusioncatcher.fusion-genes.txt`
 _ `<sample_id>.fusioncatcher.summary.txt` \* `<sample_id>.fusioncatcher.log`
 </details>
@@ -270,8 +270,8 @@ Samtools view is used to convert the chimeric SAM output from STAR_FOR_SQUID to
 <details markdown="1">
 <summary>Output files</summary>
 
--   `samtools_view_for_squid`
-    -   `<sample_id>_chimeric.bam` - sorted BAM file
+- `samtools_view_for_squid`
+  - `<sample_id>_chimeric.bam` - sorted BAM file
 
 </details>
 
@@ -282,8 +282,8 @@ Samtools sort is used to sort BAM files from STAR_FOR_ARRIBA (for arriba visuali
 <details markdown="1">
 <summary>Output files</summary>
 
--   `samtools_sort_for_<arriba/squid>`
-    -   `<sample_id>(_chimeric)_sorted.bam` - sorted BAM file
+- `samtools_sort_for_<arriba/squid>`
+  - `<sample_id>(_chimeric)_sorted.bam` - sorted BAM file
 
 </details>
 
@@ -294,8 +294,8 @@ Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visua
 <details markdown="1">
 <summary>Output files</summary>
 
--   `samtools_for_<arriba/qc>`
-    -   `<sample_id>.(Aligned.sortedByCoord).out.bam.bai` -
+- `samtools_for_<arriba/qc>`
+  - `<sample_id>.(Aligned.sortedByCoord).out.bam.bai` -
 
 </details>
 
@@ -304,12 +304,12 @@ Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visua
 <details markdown="1">
 <summary>Output files</summary>
 
--   `fusionreport`
-    -   <sample_id>
-        -   `<sample_id>.fusionreport.tsv`
-        -   `<sample_id>.fusionreport_filtered.tsv`
-        -   `index.html` - general report for all filtered fusions
-        -   `<fusion>.html` - specific report for each filtered fusion
+- `fusionreport`
+  - <sample_id>
+    - `<sample_id>.fusionreport.tsv`
+    - `<sample_id>.fusionreport_filtered.tsv`
+    - `index.html` - general report for all filtered fusions
+    - `<fusion>.html` - specific report for each filtered fusion
 
 </details>
 
@@ -318,10 +318,10 @@ Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visua
 <details markdown="1">
 <summary>Output files</summary>
 
--   `fusioninspector`
-    -   `<sample_id>.fusion_inspector_web.html` - visualisation report described in details [here](https://github.com/FusionInspector/FusionInspector/wiki/FusionInspector-Visualizations)
-    -   `FusionInspector.log`
-    -   `<sample_id>.FusionInspector.fusions.abridged.tsv`
+- `fusioninspector`
+  - `<sample_id>.fusion_inspector_web.html` - visualisation report described in details [here](https://github.com/FusionInspector/FusionInspector/wiki/FusionInspector-Visualizations)
+  - `FusionInspector.log`
+  - `<sample_id>.FusionInspector.fusions.abridged.tsv`
 
 </details>
 
@@ -330,12 +330,12 @@ Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visua
 <details markdown="1">
 <summary>Output files</summary>
 
--   `qualimap`
-    -   `qualimapReport.html` - HTML report
-    -   `rnaseq_qc_results.txt` - TXT results
-    -   `css` - dir for html style
-    -   `images_qualimapReport`- dir for html images
-    -   `raw_data_qualimapReport` - dir for html raw data
+- `qualimap`
+  - `qualimapReport.html` - HTML report
+  - `rnaseq_qc_results.txt` - TXT results
+  - `css` - dir for html style
+  - `images_qualimapReport`- dir for html images
+  - `raw_data_qualimapReport` - dir for html raw data
 
 </details>
 
@@ -346,10 +346,10 @@ Picard CollectRnaMetrics and picard MarkDuplicates share the same outpur directo
 <details markdown="1">
 <summary>Output files</summary>
 
--   `picard`
-    -   `<sample_id>.MarkDuplicates.metrics.txt` - metrics from CollectRnaMetrics
-    -   `<sample_id>_rna_metrics.txt` - metrics from MarkDuplicates
-    -   `<sample_id>.bam` - BAM file with marked duplicates
+- `picard`
+  - `<sample_id>.MarkDuplicates.metrics.txt` - metrics from CollectRnaMetrics
+  - `<sample_id>_rna_metrics.txt` - metrics from MarkDuplicates
+  - `<sample_id>.bam` - BAM file with marked duplicates
 
 </details>
 
diff --git a/docs/usage.md b/docs/usage.md
index 93ddbf0b..83895166 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -10,17 +10,17 @@ The pipeline is divided into two parts:
 
 1. Downloading and building the references, using `--build_references`: done only once and after each update.
 2. Detecting fusions using (any combination of) the following tools:
-    - arriba
-    - fusioncatcher
-    - pizzly
-    - squid
-    - starfusion
+   - arriba
+   - fusioncatcher
+   - pizzly
+   - squid
+   - starfusion
 3. QC and visualisation tools
-    - Fastqc
-    - MultiQC
-    - arriba visualisation (for fusion detected by arriba only)
-    - fusion-report
-    - fusionInspector
+   - Fastqc
+   - MultiQC
+   - arriba visualisation (for fusion detected by arriba only)
+   - fusion-report
+   - fusionInspector
 
 ### Prerequisite: download and build references
 
@@ -340,4 +340,3 @@ We recommend adding the following line to your environment to limit this (typica
 ```console
 NXF_OPTS='-Xms1g -Xmx4g'
 ```
-

From 0ef71ddee1fffc274448ea6b4b3a89a7600f1370 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Mar 2022 10:11:22 +0200
Subject: [PATCH 235/359] publish mode copy to publish_dir_mode

---
 conf/modules.config | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index f428909e..8989c3a2 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -113,7 +113,7 @@ process {
     withName: GTF_TO_REFFLAT {
         publishDir = [
             path: { "${params.genomes_base}/ensembl" },
-            mode: 'copy',
+            mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
     }
@@ -160,7 +160,7 @@ process {
     withName: SAMTOOLS_INDEX_FOR_ARRIBA {
         publishDir = [
             path: { "${params.outdir}/samtools_index_for_arriba" },
-            mode: 'copy',
+            mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
@@ -169,7 +169,7 @@ process {
         ext.when         = { !params.skip_qc && params.starfusion }
         publishDir = [
             path: { "${params.outdir}/samtools_index_for_qc" },
-            mode: 'copy',
+            mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }

From b174677245af3a841b68c2da66d9b44f8e86b03e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Mar 2022 10:13:45 +0200
Subject: [PATCH 236/359] update nf-core star module

---
 modules.json                               | 4 ++--
 modules/nf-core/modules/star/align/main.nf | 1 +
 2 files changed, 3 insertions(+), 2 deletions(-)

diff --git a/modules.json b/modules.json
index c1cbe4ab..53435c33 100644
--- a/modules.json
+++ b/modules.json
@@ -46,11 +46,11 @@
                 "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
             },
             "star/align": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+                "git_sha": "1dddf1ce9443e3d93853d86e7a7aab52e5b4d614"
             },
             "star/genomegenerate": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf
index 7c965d07..762b84f6 100644
--- a/modules/nf-core/modules/star/align/main.nf
+++ b/modules/nf-core/modules/star/align/main.nf
@@ -29,6 +29,7 @@ process STAR_ALIGN {
     tuple val(meta), path('*fastq.gz')               , optional:true, emit: fastq
     tuple val(meta), path('*.tab')                   , optional:true, emit: tab
     tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
+    tuple val(meta), path('*.out.sam')               , optional:true, emit: sam
 
     when:
     task.ext.when == null || task.ext.when

From 3de9576f6ec062fa0ce735b8798d073be486c7dc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Mar 2022 10:17:33 +0200
Subject: [PATCH 237/359] use nf-core module star/align instead of local

---
 modules/local/star/align/main.nf     | 73 ------------------------
 modules/local/star/align/meta.yml    | 85 ----------------------------
 subworkflows/local/squid_workflow.nf |  2 +-
 3 files changed, 1 insertion(+), 159 deletions(-)
 delete mode 100644 modules/local/star/align/main.nf
 delete mode 100644 modules/local/star/align/meta.yml

diff --git a/modules/local/star/align/main.nf b/modules/local/star/align/main.nf
deleted file mode 100644
index ea3868dd..00000000
--- a/modules/local/star/align/main.nf
+++ /dev/null
@@ -1,73 +0,0 @@
-process STAR_ALIGN {
-    tag "$meta.id"
-    label 'process_high'
-
-    // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null)
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0' :
-        'quay.io/biocontainers/star:2.7.9a--h9ee0642_0' }"
-
-    input:
-    tuple val(meta), path(reads)
-    path  index
-    path  gtf
-    val star_ignore_sjdbgtf
-    val seq_platform
-    val seq_center
-
-    output:
-    tuple val(meta), path('*d.out.bam')       , emit: bam
-    tuple val(meta), path('*Log.final.out')   , emit: log_final
-    tuple val(meta), path('*Log.out')         , emit: log_out
-    tuple val(meta), path('*Log.progress.out'), emit: log_progress
-    path  "versions.yml"                      , emit: versions
-
-    tuple val(meta), path('*sortedByCoord.out.bam')  , optional:true, emit: bam_sorted
-    tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript
-    tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted
-    tuple val(meta), path('*fastq.gz')               , optional:true, emit: fastq
-    tuple val(meta), path('*.tab')                   , optional:true, emit: tab
-    tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
-    tuple val(meta), path('*.out.sam')               , optional:true, emit: sam
-
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def ignore_gtf      = star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
-    def seq_platform    = seq_platform ? "'PL:$seq_platform'" : ""
-    def seq_center      = seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform "
-    def out_sam_type    = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
-    def mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
-    """
-    STAR \\
-        --genomeDir $index \\
-        --readFilesIn $reads  \\
-        --runThreadN $task.cpus \\
-        --outFileNamePrefix $prefix. \\
-        $out_sam_type \\
-        $ignore_gtf \\
-        $seq_center \\
-        $args
-
-    $mv_unsorted_bam
-
-    if [ -f ${prefix}.Unmapped.out.mate1 ]; then
-        mv ${prefix}.Unmapped.out.mate1 ${prefix}.unmapped_1.fastq
-        gzip ${prefix}.unmapped_1.fastq
-    fi
-    if [ -f ${prefix}.Unmapped.out.mate2 ]; then
-        mv ${prefix}.Unmapped.out.mate2 ${prefix}.unmapped_2.fastq
-        gzip ${prefix}.unmapped_2.fastq
-    fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        star: \$(STAR --version | sed -e "s/STAR_//g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/star/align/meta.yml b/modules/local/star/align/meta.yml
deleted file mode 100644
index 1227c1da..00000000
--- a/modules/local/star/align/meta.yml
+++ /dev/null
@@ -1,85 +0,0 @@
-name: star_align
-description: Align reads to a reference genome using STAR
-keywords:
-  - align
-  - fasta
-  - genome
-  - reference
-tools:
-  - star:
-      description: |
-        STAR is a software package for mapping DNA sequences against
-        a large reference genome, such as the human genome.
-      homepage: https://github.com/alexdobin/STAR
-      manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
-      doi: 10.1093/bioinformatics/bts635
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-        respectively.
-  - index:
-      type: directory
-      description: STAR genome index
-      pattern: "star"
-output:
-  - bam:
-      type: file
-      description: Output BAM file containing read alignments
-      pattern: "*.{bam}"
-  - log_final:
-      type: file
-      description: STAR final log file
-      pattern: "*Log.final.out"
-  - log_out:
-      type: file
-      description: STAR lot out file
-      pattern: "*Log.out"
-  - log_progress:
-      type: file
-      description: STAR log progress file
-      pattern: "*Log.progress.out"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - bam_sorted:
-      type: file
-      description: Sorted BAM file of read alignments (optional)
-      pattern: "*sortedByCoord.out.bam"
-  - bam_transcript:
-      type: file
-      description: Output BAM file of transcriptome alignment (optional)
-      pattern: "*toTranscriptome.out.bam"
-  - bam_unsorted:
-      type: file
-      description: Unsorted BAM file of read alignments (optional)
-      pattern: "*Aligned.unsort.out.bam"
-  - fastq:
-      type: file
-      description: Unmapped FastQ files (optional)
-      pattern: "*fastq.gz"
-  - tab:
-      type: file
-      description: STAR output tab file(s) (optional)
-      pattern: "*.tab"
-  - junction:
-      type: file
-      description: STAR chimeric junction output file (optional)
-      pattern: "*.out.junction"
-  - sam:
-      type: file
-      description: Output SAM file containing read alignments
-      pattern: "*.{sam}"
-
-authors:
-  - "@kevinmenden"
-  - "@drpatelh"
-  - "@praveenraj2018"
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index be2a485f..ac0ac72d 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -7,7 +7,7 @@ include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_SQUID }    from '../../modules/nf-c
 include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_SQUID }    from '../../modules/nf-core/modules/samtools/view/main'
 include { SQUID }                                       from '../../modules/local/squid/detect/main'
 include { SQUID_ANNOTATE }                              from '../../modules/local/squid/annotate/main'
-include { STAR_ALIGN as STAR_FOR_SQUID }                from '../../modules/local/star/align/main'
+include { STAR_ALIGN as STAR_FOR_SQUID }                from '../../modules/nf-core/modules/star/align/main'
 
 workflow SQUID_WORKFLOW {
     take:

From 818b5dce6ccf18e4b615922a4ca30d15f19c95bf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Mar 2022 10:24:58 +0200
Subject: [PATCH 238/359] change starfusion reference building to plug and play

---
 modules/local/starfusion/download/main.nf | 24 +++--------------------
 1 file changed, 3 insertions(+), 21 deletions(-)

diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index ecc5f7c7..5dcbe5fb 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -16,29 +16,11 @@ process STARFUSION_DOWNLOAD {
     path "*"  , emit: reference
 
     script:
-    def binPath = ( params.enable_conda ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl" )
     """
-    export TMPDIR=/tmp
+    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --no-check-certificate
 
-    wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
-    wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz -O CTAT_HumanFusionLib_Mar2021.dat.gz --no-check-certificate
-    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p --no-check-certificate
+    tar xvf CTAT_resource_lib.tar.gz
 
-    gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
-
-    $binPath \\
-        --genome_fa $fasta \\
-        --gtf $gtf \\
-        --annot_filter_rule AnnotFilterRule.pm \\
-        --fusion_annot_lib CTAT_HumanFusionLib_Mar2021.dat.gz \\
-        --pfam_db Pfam-A.hmm \\
-        --dfam_db homo_sapiens_dfam.hmm \\
-        --max_readlength $params.read_length \\
-        --CPU $task.cpus
+    rm CTAT_resource_lib.tar.gz
     """
 }

From 27cd92dc3fc76546f75300f1d861de378623f754 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Mar 2022 17:29:15 +0200
Subject: [PATCH 239/359] --fasta no longer required and --input not required
 with --build_references

---
 conf/genomes.config         |  1 -
 main.nf                     |  1 -
 modules.json                |  2 +-
 modules/local/paste/main.nf | 17 -----------------
 nextflow.config             | 33 ++++++++++++++++-----------------
 nextflow_schema.json        | 16 ++++++----------
 workflows/rnafusion.nf      | 16 +---------------
 7 files changed, 24 insertions(+), 62 deletions(-)
 delete mode 100644 modules/local/paste/main.nf

diff --git a/conf/genomes.config b/conf/genomes.config
index 92c07a6e..df2a2541 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -36,7 +36,6 @@ params {
             squid_ref                     = "${params.genomes_base}/squid"
             starfusion_ref                = "${params.genomes_base}/starfusion"
             starindex_ref                 = "${params.genomes_base}/star"
-
         }
     }
 }
diff --git a/main.nf b/main.nf
index b6c47359..e164b8fe 100644
--- a/main.nf
+++ b/main.nf
@@ -17,7 +17,6 @@ nextflow.enable.dsl = 2
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-params.fasta             = WorkflowMain.getGenomeAttribute(params, 'fasta')
 params.genomes_base      = WorkflowMain.getGenomeAttribute(params, 'genomes_base')
 
 
diff --git a/modules.json b/modules.json
index 53435c33..042f1efd 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/local/paste/main.nf b/modules/local/paste/main.nf
deleted file mode 100644
index b1a89cb9..00000000
--- a/modules/local/paste/main.nf
+++ /dev/null
@@ -1,17 +0,0 @@
-process PASTE {
-    tag "$meta.id"
-    label 'process_medium'
-
-    input:
-    path input
-
-    output:
-    path output
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    paste ${input} ${input} | cut -f12,16-25 > ${refflat}
-    """
-}
diff --git a/nextflow.config b/nextflow.config
index 7c04ce3f..d5ff5233 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -10,8 +10,8 @@
 params {
 
     // Input options
-    input                      = null
-    fasta                      = null
+    input                      = "fake_input_to_build_refs.csv"
+    fasta                      = "${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
     build_references           = false
     cosmic_username            = null
     cosmic_passwd              = null
@@ -23,7 +23,7 @@ params {
 
     // Genome
     genome                     = 'GRCh38'
-    genomes_base               = null
+    genomes_base               = "${params.outdir}/references"
     ensembl_version            = 105
     read_length                = 100
     genomes                    = [:]
@@ -37,7 +37,6 @@ params {
     // Enable or disable tools
     all                        = false
     arriba                     = false
-    ericscript                 = false
     fusioncatcher              = false
     pizzly                     = false
     squid                      = false
@@ -52,7 +51,6 @@ params {
     // Path to references
     arriba_ref                    = "${params.genomes_base}/arriba"
     ensembl_ref                   = "${params.genomes_base}/ensembl"
-    ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v102"
     pizzly_ref                    = "${params.genomes_base}/pizzly"
     squid_ref                     = "${params.genomes_base}/squid"
@@ -69,18 +67,19 @@ params {
     fusioncatcher_fusions         = null
 
     // Boilerplate options
-    outdir                     = null
-    tracedir                   = "${params.outdir}/pipeline_info"
-    publish_dir_mode           = 'copy'
-    email                      = null
-    email_on_fail              = null
-    plaintext_email            = false
-    monochrome_logs            = false
-    help                       = false
-    validate_params            = true
-    show_hidden_params         = false
-    schema_ignore_params       = 'genomes'
-    enable_conda               = false
+    outdir                            = './results'
+    tracedir                          = "${params.outdir}/pipeline_info"
+    publish_dir_mode                  = 'copy'
+    email                             = null
+    email_on_fail                     = null
+    plaintext_email                   = false
+    monochrome_logs                   = false
+    help                              = false
+    validate_params                   = true
+    show_hidden_params                = false
+    schema_ignore_params              = 'genomes'
+    enable_conda                      = false
+    singularity_pull_docker_container = false
 
     // Config options
     custom_config_version      = 'master'
diff --git a/nextflow_schema.json b/nextflow_schema.json
index d626720d..475e8fdb 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -103,16 +103,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to ensembl references"
                 },
-                "ericscript": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Build or run ericscript references/analyses"
-                },
-                "ericscript_ref": {
-                    "type": "string",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to ericscript references"
-                },
                 "fusioncatcher": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
@@ -394,6 +384,12 @@
                     "hidden": true,
                     "fa_icon": "fas fa-bacon"
                 },
+                "singularity_pull_docker_container": {
+                    "type": "boolean",
+                    "description": "Use to pull docker containers to run with singularity",
+                    "hidden": true,
+                    "fa_icon": "fas fa-bacon"
+                },
                 "seq_center": {
                     "type": "boolean",
                     "description": "Sequencing center",
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index b5d5a261..6d3aa53a 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -9,23 +9,9 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
 // Validate input parameters
 WorkflowRnafusion.initialise(params, log)
 
-// TODO nf-core: Add all file path parameters for the pipeline to the list below
-// Check input path parameters to see if they exist
-
-
-def checkPathParamList = [
-    params.input, params.multiqc_config,
-    params.fasta, params.genomes_base,
-    params.ensembl_ref,
-    params.fusioncatcher_ref, params.starfusion_ref,
-    params.arriba_ref, params.starindex_ref,
-    params.ensembl_version
-]
-
-
 // Check mandatory parameters
-if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' }
 
+if (file(params.input).exists() || params.build_references) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet does not exist or was not specified!' }
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From c2da353fe0d55e6a7f4b32b30a55d1fe16cd2ae8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 29 Mar 2022 09:32:19 +0200
Subject: [PATCH 240/359] Update docs/output.md

Co-authored-by: Anders Jemt <jemten@users.noreply.github.com>
---
 docs/output.md | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 69bc1387..b9d64d87 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -22,8 +22,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 - [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report
 - [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman and FusionGBD databases
 - [FusionInspector](#fusionInspector) - IGV-based visualisation tool for fusions filtered by fusion-report
-- [Qualimap](#qualimap) - quality control of alignment
-- [Picard](#picard) - collect metrics
+- [Qualimap](#qualimap) - Quality control of alignment
+- [Picard](#picard) - Collect metrics
 - [FastQC](#fastqc) - Raw read quality control
 - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
 - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution

From 7bad769a4c786415e1e6d4ab1f50bd687a19cd4f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 29 Mar 2022 11:06:10 +0200
Subject: [PATCH 241/359] rearranged CHANGELOG

---
 CHANGELOG.md | 22 ++++++----------------
 1 file changed, 6 insertions(+), 16 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 43a81358..3fac7e79 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -6,21 +6,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ## [2.0.0] nfcore/rnafusion - 2022/03/??
 
 Update to DSL2 and newer software/reference versions
-
-### Non-DSL2 related changes
-
-- replace gtf file with chr.gtf: Please confirm if this change is ok for the whole pipeline
-- remove subdirectory with params.genome as only GRCh38 is supported (not necessary but helped me see the output more clearly, can be reversed)
-- modification of output parameters to make sure every important file is published
-- additional build_references flag to trigger the workflow to build references
-- container for starfusion is changed and the absolute path to prep_genome_lib.pl is given
-  --fasta argument is not required when executing the workflow to build references
-- increase cpu + memory for process STARFUSION_DOWNLOAD
-- ci test done on stubs of reference building subprocesses ensembl and arriba
-- **For the moment, the rnafusion workflow (to analyse data and not build the references) is removed as dependencies and links would have to be fixed in the rnafusion workflow for execution of the build references otherwise**
-
----
-
 ### Added
 
 - Added `qualimap/rnaseq v2.2.2d` from nf-core modules
@@ -44,6 +29,11 @@ Update to DSL2 and newer software/reference versions
 - Upgrade `arriba` references from `v1.2.0` to `v2.1.0`
 - Upgrade `fusioncatcher` references from `v98` to `v102`
 - Use `arriba` (detect only), `kallisto` and `STAR` from nf-core modules
+- Instead of separate script to build the references, added `--build_references` argument in the main
+- `--fasta` argument is not required with `--build_references` and set by default to the ensembl references built in the detection workflow
+- CI test done on stubs of reference building for subprocesses ensembl and arriba
+
+
 
 Parameters for `STAR` for `arriba` changed from:
 
@@ -151,7 +141,7 @@ to
 ### Removed
 
 - Ericscript tool
-- GRCh37 support
+- GRCh37 support. Subdirectory with params.genome are removed
 - Running with conda
 
 ## v1.3.0dev nfcore/rnafusion - 2020/07/15

From 16d570e265f9dc5a7a062cc685b3f3c8dc641482 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 11:06:33 +0200
Subject: [PATCH 242/359] prettier linting

---
 CHANGELOG.md | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3fac7e79..515b6801 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -6,6 +6,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ## [2.0.0] nfcore/rnafusion - 2022/03/??
 
 Update to DSL2 and newer software/reference versions
+
 ### Added
 
 - Added `qualimap/rnaseq v2.2.2d` from nf-core modules
@@ -33,8 +34,6 @@ Update to DSL2 and newer software/reference versions
 - `--fasta` argument is not required with `--build_references` and set by default to the ensembl references built in the detection workflow
 - CI test done on stubs of reference building for subprocesses ensembl and arriba
 
-
-
 Parameters for `STAR` for `arriba` changed from:
 
 ```bash

From 2ab993a828a6cb5e5187ba672349a7ce22107143 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 14:19:33 +0200
Subject: [PATCH 243/359] change links ctat lib for starfusion download

---
 modules/local/starfusion/download/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 5dcbe5fb..efe384fb 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -19,8 +19,8 @@ process STARFUSION_DOWNLOAD {
     """
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --no-check-certificate
 
-    tar xvf CTAT_resource_lib.tar.gz
+    tar xvf GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz
 
-    rm CTAT_resource_lib.tar.gz
+    rm GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz
     """
 }

From 9b238254f8e96c58ab0d0e4306db7942bf9f7120 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 14:19:56 +0200
Subject: [PATCH 244/359] gtftogenepred to low process

---
 modules/local/uscs/custom_gtftogenepred/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/uscs/custom_gtftogenepred/main.nf b/modules/local/uscs/custom_gtftogenepred/main.nf
index 1b2a22bc..93ce49b1 100644
--- a/modules/local/uscs/custom_gtftogenepred/main.nf
+++ b/modules/local/uscs/custom_gtftogenepred/main.nf
@@ -1,5 +1,5 @@
 process GTF_TO_REFFLAT {
-    label 'process_medium'
+    label 'process_low'
 
     conda (params.enable_conda ? "bioconda::ucsc-gtftogenepred=377" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?

From 6d79a694daade7e5cdbffb6b5394d14022251e3a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 16:56:20 +0200
Subject: [PATCH 245/359] remove completed TODO

---
 bin/check_samplesheet.py | 1 -
 1 file changed, 1 deletion(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index a1be0901..2469c07e 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -1,6 +1,5 @@
 #!/usr/bin/env python
 
-# TODO nf-core: Update the script to check the samplesheet
 # This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv
 
 import os

From 6c00c4db74b591f78a309a9860d604c0e3821139 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 16:56:42 +0200
Subject: [PATCH 246/359] update genoms.config

---
 conf/genomes.config | 4 ----
 1 file changed, 4 deletions(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index df2a2541..3fdce2aa 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -16,11 +16,9 @@ params {
             transcript                    = "${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
             arriba_ref                    = "${params.genomes_base}/arriba"
             ensembl_ref                   = "${params.genomes_base}/ensembl"
-            ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
             fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
             pizzly_ref                    = "${params.genomes_base}/pizzly"
             squid_ref                     = "${params.genomes_base}/squid"
-            starfusion_ref                = "${params.genomes_base}/starfusion"
             starindex_ref                 = "${params.genomes_base}/star"
 
         }
@@ -30,11 +28,9 @@ params {
             transcript                    = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.cdna.all.fa.gz"
             arriba_ref                    = "${params.genomes_base}/arriba"
             ensembl_ref                   = "${params.genomes_base}/ensembl"
-            ericscript_ref                = "${params.genomes_base}/ericscript/homo_sapiens"
             fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
             pizzly_ref                    = "${params.genomes_base}/pizzly"
             squid_ref                     = "${params.genomes_base}/squid"
-            starfusion_ref                = "${params.genomes_base}/starfusion"
             starindex_ref                 = "${params.genomes_base}/star"
         }
     }

From 6099a03366e64b9ad46730c4ab1d75dd53a2aef2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 16:57:03 +0200
Subject: [PATCH 247/359] update genoms.config

---
 conf/modules.config | 2 --
 1 file changed, 2 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 8989c3a2..314838bf 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -196,7 +196,6 @@ process {
         ext.args = { "-Sb -o ${meta.id}_chimeric.bam" }
     }
 
-//TODO document changes from previous rnafusion version
     withName: STAR_FOR_ARRIBA {
         publishDir = [
             path: { "${params.outdir}/star_for_arriba" },
@@ -238,7 +237,6 @@ process {
         --readFilesCommand zcat'
     }
 
-//TODO document changes from previous rnafusion version to use star-fusion recommendations
     withName: STAR_FOR_STARFUSION {
         publishDir = [
             path: { "${params.outdir}/star_for_starfusion" },

From 329cfc0f3f137ba17d3d18f58d1de7ee71c1bad0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 16:57:55 +0200
Subject: [PATCH 248/359] remove completed TODO

---
 docs/usage.md                                   | 10 +++++-----
 modules.json                                    |  2 +-
 modules/local/uscs/custom_gtftogenepred/main.nf |  3 ---
 modules/nf-core/modules/kallisto/index/main.nf  |  2 +-
 nextflow.config                                 |  9 +++++++--
 subworkflows/local/arriba_workflow.nf           |  6 +-----
 subworkflows/local/fusioninspector_workflow.nf  |  4 +---
 subworkflows/local/pizzly_workflow.nf           |  4 +---
 subworkflows/local/qc_workflow.nf               |  8 ++------
 subworkflows/local/squid_workflow.nf            |  9 ++-------
 subworkflows/local/starfusion_workflow.nf       | 10 ++--------
 workflows/build_references.nf                   | 10 ++--------
 12 files changed, 25 insertions(+), 52 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 74ad45bd..a18be898 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -48,7 +48,7 @@
   - [--gtf](#--gtf)
   - [--star_index](#--star_index)
   - [--star_fusion_ref](#--star_fusion_ref)
-  - [--transcript](#--transcript)
+  - [--](#--)
 - [Other command line parameters](#other-command-line-parameters)
   - [--debug](#--debug)
   - [--read_length](#--read_length)
@@ -358,7 +358,7 @@ As you will see in the example below, we have:
 params {
     modules {
         'star_align' {
-            args          = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16"
+            args          = "--quantMode omeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantomeBan Singleend --outFilterMismatchNmax 16"
             publishDir    = "my_star_directory"
             publish_files = ['out':'log', 'tab':'log', 'bam':'']
         }
@@ -594,12 +594,12 @@ Required reference in order to run `STAR-Fusion`.
 --star_fusion_ref '<path to STAR-Fusion reference>'
 ```
 
-### --transcript
+### --
 
-Required transcript file.
+Required  file.
 
 ```bash
---transcript '<path to transcript reference>'
+-- '<path to  reference>'
 ```
 
 ## Other command line parameters
diff --git a/modules.json b/modules.json
index 042f1efd..53435c33 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/local/uscs/custom_gtftogenepred/main.nf b/modules/local/uscs/custom_gtftogenepred/main.nf
index 93ce49b1..74e4c6f3 100644
--- a/modules/local/uscs/custom_gtftogenepred/main.nf
+++ b/modules/local/uscs/custom_gtftogenepred/main.nf
@@ -6,15 +6,12 @@ process GTF_TO_REFFLAT {
         'https://depot.galaxyproject.org/singularity/ucsc-gtftogenepred:377--ha8a8165_5' :
         'quay.io/biocontainers/ucsc-gtftogenepred:377--ha8a8165_5' }"
 
-//TODO easier with meta/or add to ensembl
     input:
     path gtf
 
     output:
     path('*.refflat'), emit: refflat
 
-
-    // TODO:add version
     script:
     def genepred = gtf + '.genepred'
     def refflat = gtf + '.refflat'
diff --git a/modules/nf-core/modules/kallisto/index/main.nf b/modules/nf-core/modules/kallisto/index/main.nf
index 0f10e564..4136486b 100644
--- a/modules/nf-core/modules/kallisto/index/main.nf
+++ b/modules/nf-core/modules/kallisto/index/main.nf
@@ -5,7 +5,7 @@ process KALLISTO_INDEX {
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1' :
-        'quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1' }"
+        'quay.io/biocontainers/kallisto:0.48.0--h0d531b0_1' }"
 
     input:
     path fasta
diff --git a/nextflow.config b/nextflow.config
index d5ff5233..c1417e05 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -11,7 +11,7 @@ params {
 
     // Input options
     input                      = "fake_input_to_build_refs.csv"
-    fasta                      = "${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
+    fasta                      = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.all.fa"
     build_references           = false
     cosmic_username            = null
     cosmic_passwd              = null
@@ -27,6 +27,11 @@ params {
     ensembl_version            = 105
     read_length                = 100
     genomes                    = [:]
+    gtf                        = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.gtf"
+    chrgtf                     = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.chr.gtf"
+    transcript                 = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.cdna.all.fa.gz"
+    refflat                    = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.chr.gtf.refflat"
+
 
     // Alignment options
     star_ignore_sjdbgtf        = false
@@ -54,7 +59,7 @@ params {
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v102"
     pizzly_ref                    = "${params.genomes_base}/pizzly"
     squid_ref                     = "${params.genomes_base}/squid"
-    starfusion_ref                = "${params.genomes_base}/starfusion"
+    starfusion_ref                = "${params.genomes_base}/starfusion/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play"
     starindex_ref                 = "${params.genomes_base}/star"
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
 
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 8c78e28a..01f9753f 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -22,12 +22,8 @@ workflow ARRIBA_WORKFLOW {
         ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
         if (params.arriba) {
-            gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-            star_ignore_sjdbgtf = false
-            seq_platform = false
-            seq_center = false
 
-            STAR_FOR_ARRIBA( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+            STAR_FOR_ARRIBA( reads, index, params.gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
 
             SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam)
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index aeb5bbb5..e98577d7 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -12,14 +12,12 @@ workflow FUSIONINSPECTOR_WORKFLOW {
 
     main:
         ch_versions = Channel.empty()
-        index ="${params.starfusion_ref}/ctat_genome_lib_build_dir"
+        index ="${params.starfusion_ref}"
 
         FUSIONINSPECTOR( reads, fusion_list , index )
         ch_versions = ch_versions.mix(FUSIONINSPECTOR.out.versions)
 
     emit:
         versions        = ch_versions.ifEmpty(null)
-        // fusion_list     = FUSIONINSPECTOR.out.fusion_list
-
 }
 
diff --git a/subworkflows/local/pizzly_workflow.nf b/subworkflows/local/pizzly_workflow.nf
index 52bdd44b..e1a74178 100644
--- a/subworkflows/local/pizzly_workflow.nf
+++ b/subworkflows/local/pizzly_workflow.nf
@@ -20,13 +20,11 @@ workflow PIZZLY_WORKFLOW {
                 ch_pizzly_fusions = params.pizzly_fusions
             } else {
                 index ="${params.pizzly_ref}/kallisto"
-                gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-                transcript ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
 
                 KALLISTO_QUANT( reads, index )
                 ch_versions = ch_versions.mix(KALLISTO_QUANT.out.versions)
 
-                PIZZLY( KALLISTO_QUANT.out.txt, transcript, gtf )
+                PIZZLY( KALLISTO_QUANT.out.txt, params.transcript, params.gtf )
                 ch_versions = ch_versions.mix(PIZZLY.out.versions)
 
                 GET_PATH(PIZZLY.out.fusions)
diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index c97a1093..701a1c30 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -15,10 +15,7 @@ workflow QC_WORKFLOW {
         ch_versions = Channel.empty()
         ch_qualimap_qc = Channel.empty()
 
-        gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
-        refflat ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf.refflat"
-
-        QUALIMAP_RNASEQ(bam_sorted, gtf)
+        QUALIMAP_RNASEQ(bam_sorted, params.gtf)
         ch_versions = ch_versions.mix(QUALIMAP_RNASEQ.out.versions)
         ch_qualimap_qc = QUALIMAP_RNASEQ.out.results.ifEmpty(null)
 
@@ -27,13 +24,12 @@ workflow QC_WORKFLOW {
 
         bam_indexed = bam_sorted.join(SAMTOOLS_INDEX_FOR_QC.out.bai)
 
-        PICARD_COLLECTRNASEQMETRICS(bam_indexed, refflat, [])
+        PICARD_COLLECTRNASEQMETRICS(bam_indexed, paramsrefflat, [])
         ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
 
         PICARD_MARKDUPLICATES(bam_sorted)
         ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES.out.versions)
 
-// TODO: check what happens with skip qc
     emit:
         versions            = ch_versions.ifEmpty(null)
         qualimap_qc         = ch_qualimap_qc.ifEmpty(null)
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index ac0ac72d..e017d31f 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -22,13 +22,8 @@ workflow SQUID_WORKFLOW {
             if (params.squid_fusions){
                 ch_squid_fusions = params.squid_fusions
             } else {
-            gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-            star_ignore_sjdbgtf = false
-            seq_platform = false
-            seq_center = false
-            index = params.starindex_ref
 
-            STAR_FOR_SQUID( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+            STAR_FOR_SQUID( reads, params.starindex_ref, params.gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
             SAMTOOLS_VIEW_FOR_SQUID ( STAR_FOR_SQUID.out.sam, [] )
@@ -42,7 +37,7 @@ workflow SQUID_WORKFLOW {
             SQUID ( bam_sorted )
             ch_versions = ch_versions.mix(SQUID.out.versions)
 
-            SQUID_ANNOTATE ( SQUID.out.fusions, gtf )
+            SQUID_ANNOTATE ( SQUID.out.fusions, params.gtf )
             ch_versions = ch_versions.mix(SQUID_ANNOTATE.out.versions)
 
             GET_PATH(SQUID_ANNOTATE.out.fusions_annotated)
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index f25227cc..4a621d02 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -20,20 +20,14 @@ workflow STARFUSION_WORKFLOW {
             if (params.starfusion_fusions){
                 ch_starfusion_fusions = params.starfusion_fusions
             } else {
-                gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
-                ref ="${params.starfusion_ref}/ctat_genome_lib_build_dir"
 
-                star_ignore_sjdbgtf = false
-                seq_platform = false
-                seq_center = false
-
-                STAR_FOR_STARFUSION( reads, params.starindex_ref, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
+                STAR_FOR_STARFUSION( reads, params.starindex_ref, params.chrgtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
                 ch_align = STAR_FOR_STARFUSION.out.bam_sorted
 
                 reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
 
-                STARFUSION( reads_junction, ref)
+                STARFUSION( reads_junction, params.starfusion_ref)
                 ch_versions = ch_versions.mix(STARFUSION.out.versions)
 
                 GET_PATH(STARFUSION.out.fusions)
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index a3061216..0a59c4f9 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -6,7 +6,6 @@
 
 include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'
 include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'
-include { ERICSCRIPT_DOWNLOAD }             from '../modules/local/ericscript/download/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/download/main'
 include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
@@ -31,9 +30,7 @@ workflow BUILD_REFERENCES {
 
     ENSEMBL_DOWNLOAD( params.ensembl_version )
 
-    GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
-
-    if (params.starindex || params.all) {
+    if (params.starindex || params.all || params.starfusion || params.arriba || params.squid ) {
         STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
     }
 
@@ -41,10 +38,6 @@ workflow BUILD_REFERENCES {
         ARRIBA_DOWNLOAD()
     }
 
-    if (params.ericscript || params.all) {
-        ERICSCRIPT_DOWNLOAD()
-    }
-
     if (params.fusioncatcher || params.all) {
         FUSIONCATCHER_DOWNLOAD()
     }
@@ -54,6 +47,7 @@ workflow BUILD_REFERENCES {
     }
 
     if (params.starfusion || params.all) {
+        GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
         STARFUSION_DOWNLOAD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
     }
 

From 1eca9dd6dd8c964085eae1290cc307607b5647d8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 17:01:34 +0200
Subject: [PATCH 249/359] typo

---
 subworkflows/local/qc_workflow.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index 701a1c30..c55c3821 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -24,7 +24,7 @@ workflow QC_WORKFLOW {
 
         bam_indexed = bam_sorted.join(SAMTOOLS_INDEX_FOR_QC.out.bai)
 
-        PICARD_COLLECTRNASEQMETRICS(bam_indexed, paramsrefflat, [])
+        PICARD_COLLECTRNASEQMETRICS(bam_indexed, params.refflat, [])
         ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
 
         PICARD_MARKDUPLICATES(bam_sorted)

From 7cd3778ed43f2c6f57ad38896590fe9f28cc126c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 17:04:21 +0200
Subject: [PATCH 250/359] prettier

---
 docs/usage.md                               | 2 +-
 modules.json                                | 2 +-
 modules/local/fusionreport/download/main.nf | 3 ---
 3 files changed, 2 insertions(+), 5 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index a18be898..b8f5a882 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -596,7 +596,7 @@ Required reference in order to run `STAR-Fusion`.
 
 ### --
 
-Required  file.
+Required file.
 
 ```bash
 -- '<path to  reference>'
diff --git a/modules.json b/modules.json
index 53435c33..042f1efd 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index ae51f880..746c3465 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -17,9 +17,6 @@ process FUSIONREPORT_DOWNLOAD {
     path "*"                , emit: reference
     path "versions.yml"     , emit: versions
 
-
-    //TODO: update mitelman to take DB from https://storage.googleapis.com/mitelman-data-files/prod/mitelman_db.zip
-    //TODO: update fusionGDB to GRCh38
     script:
     """
     fusion_report download --cosmic_usr $username --cosmic_passwd $passwd .

From 94f7cd884ba70c7a5febd32eb4749476707a1df9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 31 Mar 2022 17:08:20 +0200
Subject: [PATCH 251/359] update nf-core modules

---
 modules/nf-core/modules/fastqc/main.nf              | 12 ++++++++++++
 modules/nf-core/modules/multiqc/main.nf             | 12 ++++++++++++
 .../nf-core/modules/picard/markduplicates/main.nf   | 13 +++++++++++++
 modules/nf-core/modules/samtools/faidx/main.nf      | 10 ++++++++++
 modules/nf-core/modules/samtools/index/main.nf      | 12 ++++++++++++
 modules/nf-core/modules/samtools/sort/main.nf       | 11 +++++++++++
 6 files changed, 70 insertions(+)

diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf
index ed6b8c50..05730368 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/modules/fastqc/main.nf
@@ -44,4 +44,16 @@ process FASTQC {
         END_VERSIONS
         """
     }
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.html
+    touch ${prefix}.zip
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
index 1264aac1..ae019dbf 100644
--- a/modules/nf-core/modules/multiqc/main.nf
+++ b/modules/nf-core/modules/multiqc/main.nf
@@ -28,4 +28,16 @@ process MULTIQC {
         multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
     END_VERSIONS
     """
+
+    stub:
+    """
+    touch multiqc_data
+    touch multiqc_plots
+    touch multiqc_report.html
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf
index 5196b6ed..e754a587 100644
--- a/modules/nf-core/modules/picard/markduplicates/main.nf
+++ b/modules/nf-core/modules/picard/markduplicates/main.nf
@@ -42,4 +42,17 @@ process PICARD_MARKDUPLICATES {
         picard: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:)
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.bam
+    touch ${prefix}.bam.bai
+    touch ${prefix}.MarkDuplicates.metrics.txt
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        picard: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:)
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf
index 7732a4ec..053279ff 100644
--- a/modules/nf-core/modules/samtools/faidx/main.nf
+++ b/modules/nf-core/modules/samtools/faidx/main.nf
@@ -29,4 +29,14 @@ process SAMTOOLS_FAIDX {
         samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
     END_VERSIONS
     """
+
+    stub:
+    """
+    touch ${fasta}.fai
+    cat <<-END_VERSIONS > versions.yml
+
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf
index e41cdcc8..fff6e1b8 100644
--- a/modules/nf-core/modules/samtools/index/main.nf
+++ b/modules/nf-core/modules/samtools/index/main.nf
@@ -33,4 +33,16 @@ process SAMTOOLS_INDEX {
         samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
     END_VERSIONS
     """
+
+    stub:
+    """
+    touch ${input}.bai
+    touch ${input}.crai
+    touch ${input}.csi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf
index 0e2de8ba..ba46f0c9 100644
--- a/modules/nf-core/modules/samtools/sort/main.nf
+++ b/modules/nf-core/modules/samtools/sort/main.nf
@@ -28,4 +28,15 @@ process SAMTOOLS_SORT {
         samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.bam
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
 }

From 8bed2627db8ebe86f85db20dfac3356fa758b3f5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 12:02:21 +0200
Subject: [PATCH 252/359] update nf-core modules sha5 and genomes.conf

---
 conf/genomes.config | 25 ++++---------------------
 modules.json        | 14 +++++++-------
 2 files changed, 11 insertions(+), 28 deletions(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index 3fdce2aa..c7b30bc0 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -11,27 +11,10 @@
 params {
     genomes {
         'GRCh38' {
-            fasta                         = "${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
-            gtf                           = "${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-            transcript                    = "${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
-            arriba_ref                    = "${params.genomes_base}/arriba"
-            ensembl_ref                   = "${params.genomes_base}/ensembl"
-            fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
-            pizzly_ref                    = "${params.genomes_base}/pizzly"
-            squid_ref                     = "${params.genomes_base}/squid"
-            starindex_ref                 = "${params.genomes_base}/star"
-
-        }
-        'GRCh37' {
-            fasta                         = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.all.fa"
-            gtf                           = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.gtf"
-            transcript                    = "${params.genomes_base}/Homo_sapiens.GRCh37.${params.ensembl_version}.cdna.all.fa.gz"
-            arriba_ref                    = "${params.genomes_base}/arriba"
-            ensembl_ref                   = "${params.genomes_base}/ensembl"
-            fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/current"
-            pizzly_ref                    = "${params.genomes_base}/pizzly"
-            squid_ref                     = "${params.genomes_base}/squid"
-            starindex_ref                 = "${params.genomes_base}/star"
+            fasta                         = "${params.genomes_base}/ensembl/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
+            gtf                           = "${params.genomes_base}/ensembl/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
+            chrgtf                        = "${params.genomes_base}/ensembl/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
+            transcript                    = "${params.genomes_base}/ensembl/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
         }
     }
 }
diff --git a/modules.json b/modules.json
index 042f1efd..ac19fc71 100644
--- a/modules.json
+++ b/modules.json
@@ -13,19 +13,19 @@
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "fastqc": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
             },
             "kallisto/index": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "multiqc": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
             },
             "picard/collectwgsmetrics": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "picard/markduplicates": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
             },
             "qualimap/rnaseq": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
@@ -34,13 +34,13 @@
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "samtools/faidx": {
-                "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
+                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
             },
             "samtools/index": {
-                "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
+                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
             },
             "samtools/sort": {
-                "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
+                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
             },
             "samtools/view": {
                 "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
@@ -53,4 +53,4 @@
             }
         }
     }
-}
+}
\ No newline at end of file

From fb91a3b776c8a7bfe0a4a23661e5a4b779f095da Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 12:02:41 +0200
Subject: [PATCH 253/359] remove completed TODO

---
 modules/local/fusioninspector/meta.yml | 1 -
 1 file changed, 1 deletion(-)

diff --git a/modules/local/fusioninspector/meta.yml b/modules/local/fusioninspector/meta.yml
index acf3e0bb..cc03239b 100644
--- a/modules/local/fusioninspector/meta.yml
+++ b/modules/local/fusioninspector/meta.yml
@@ -26,7 +26,6 @@ input:
       description: Path to ctat references
       pattern: "*"
 
-# TODO complete output
 output:
   - versions:
       type: file

From 39166374b0a0918c8d28e7b398a11b09167f58b5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 12:03:44 +0200
Subject: [PATCH 254/359] updates to pizzly

---
 modules/local/pizzly/detect/main.nf | 15 +++++++--------
 1 file changed, 7 insertions(+), 8 deletions(-)

diff --git a/modules/local/pizzly/detect/main.nf b/modules/local/pizzly/detect/main.nf
index 376dc7f2..6222d3ba 100644
--- a/modules/local/pizzly/detect/main.nf
+++ b/modules/local/pizzly/detect/main.nf
@@ -3,11 +3,9 @@ process PIZZLY {
     label 'process_medium'
 
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2 bioconda::pizzly==0.37.3" : null)
-    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
-        container "https://depot.galaxyproject.org/singularity/pizzly:0.37.3--py36_2"
-    } else {
-        container "quay.io/biocontainers/pizzly:0.37.3--h470a237_3"
-    }
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/pizzly:0.37.3--py36_2' :
+        'quay.io/biocontainers/pizzly:0.37.3--h470a237_3' }"
 
     input:
     tuple val(meta), path(txt)
@@ -15,9 +13,9 @@ process PIZZLY {
     path gtf
 
     output:
-    path "versions.yml"                              , emit: versions
-    tuple val(meta), path('*pizzly.txt')            , emit: fusions
-    tuple val(meta), path('*unfiltered.json')        , emit: fusions_unfiltered
+    path "versions.yml"                         , emit: versions
+    tuple val(meta), path("*pizzly.txt")        , emit: fusions
+    tuple val(meta), path("*unfiltered.json")   , emit: fusions_unfiltered
 
     script:
     def args = task.ext.args ?: ''
@@ -37,3 +35,4 @@ process PIZZLY {
     END_VERSIONS
     """
 }
+

From 9dbd1c36be70b19b9c2aec3f8727826fdf9d9a92 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 12:06:43 +0200
Subject: [PATCH 255/359] add gtf, chrgtf, transcript and refflat as input
 parameters

---
 nextflow.config      | 14 +++++++-------
 nextflow_schema.json | 32 ++++++++++++++++++++++++++++++++
 2 files changed, 39 insertions(+), 7 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index c1417e05..243fff5c 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -11,7 +11,7 @@ params {
 
     // Input options
     input                      = "fake_input_to_build_refs.csv"
-    fasta                      = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.all.fa"
+    fasta                      = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"
     build_references           = false
     cosmic_username            = null
     cosmic_passwd              = null
@@ -27,10 +27,10 @@ params {
     ensembl_version            = 105
     read_length                = 100
     genomes                    = [:]
-    gtf                        = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.gtf"
-    chrgtf                     = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.chr.gtf"
-    transcript                 = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.cdna.all.fa.gz"
-    refflat                    = "${params.ensembl_ref}/Homo_sapiens.{params.genome}.${params.ensembl_version}.chr.gtf.refflat"
+    gtf                        = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf"
+    chrgtf                     = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf"
+    transcript                 = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.cdna.all.fa.gz"
+    refflat                    = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf.refflat"
 
 
     // Alignment options
@@ -54,10 +54,10 @@ params {
     skip_vis                   = false
 
     // Path to references
-    arriba_ref                    = "${params.genomes_base}/arriba"
     ensembl_ref                   = "${params.genomes_base}/ensembl"
+    arriba_ref                    = "${params.genomes_base}/arriba"
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v102"
-    pizzly_ref                    = "${params.genomes_base}/pizzly"
+    pizzly_ref                    = "${params.genomes_base}/pizzly/kallisto"
     squid_ref                     = "${params.genomes_base}/squid"
     starfusion_ref                = "${params.genomes_base}/starfusion/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play"
     starindex_ref                 = "${params.genomes_base}/star"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 475e8fdb..17f23e1b 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -215,6 +215,38 @@
                     "description": "Path to FASTA genome file.",
                     "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.",
                     "fa_icon": "far fa-file-code"
+                },
+                "gtf": {
+                    "type": "string",
+                    "format": "file-path",
+                    "mimetype": "text/plain",
+                    "pattern": "^\\S+\\.gtf?(\\.gz)?$",
+                    "description": "Path to GTF genome file.",
+                    "fa_icon": "far fa-file-code"
+                },
+                "chrgtf": {
+                    "type": "string",
+                    "format": "file-path",
+                    "mimetype": "text/plain",
+                    "pattern": "^\\S+\\.chr.gtf?(\\.gz)?$",
+                    "description": "Path to GTF genome file.",
+                    "fa_icon": "far fa-file-code"
+                },
+                "transcript": {
+                    "type": "string",
+                    "format": "file-path",
+                    "mimetype": "text/plain",
+                    "pattern":  "^\\S+\\.fn?a(sta)?(\\.gz)?$",
+                    "description": "Path to GTF genome file.",
+                    "fa_icon": "far fa-file-code"
+                },
+                "refflat": {
+                    "type": "string",
+                    "format": "file-path",
+                    "mimetype": "text/plain",
+                    "pattern":  "^\\S+\\.refflat?$",
+                    "description": "Path to GTF genome file.",
+                    "fa_icon": "far fa-file-code"
                 }
             }
         },

From be35b6024c0de7b5b7f1f2d3bbd5e837fbe91586 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 12:07:41 +0200
Subject: [PATCH 256/359] adapt pizzly workflow to use parameters

---
 subworkflows/local/pizzly_workflow.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/subworkflows/local/pizzly_workflow.nf b/subworkflows/local/pizzly_workflow.nf
index e1a74178..452d5a7f 100644
--- a/subworkflows/local/pizzly_workflow.nf
+++ b/subworkflows/local/pizzly_workflow.nf
@@ -19,9 +19,8 @@ workflow PIZZLY_WORKFLOW {
             if (params.pizzly_fusions) {
                 ch_pizzly_fusions = params.pizzly_fusions
             } else {
-                index ="${params.pizzly_ref}/kallisto"
 
-                KALLISTO_QUANT( reads, index )
+                KALLISTO_QUANT( reads, params.pizzly_ref )
                 ch_versions = ch_versions.mix(KALLISTO_QUANT.out.versions)
 
                 PIZZLY( KALLISTO_QUANT.out.txt, params.transcript, params.gtf )

From e0328978cab663b812a5ff947cbf485f628d165b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 12:08:23 +0200
Subject: [PATCH 257/359] update workflows/build_references.nf for new
 onComplete workfllow

---
 workflows/build_references.nf | 5 +++--
 1 file changed, 3 insertions(+), 2 deletions(-)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 0a59c4f9..1227ed04 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -64,8 +64,9 @@ workflow BUILD_REFERENCES {
 */
 
 workflow.onComplete {
-    def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
-    NfcoreTemplate.email(workflow, params, summary_params, projectDir, log)
+    if (params.email || params.email_on_fail) {
+        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
+    }
     NfcoreTemplate.summary(workflow, params, log)
 }
 

From 745e0def2e3dda2b158ca514eea7dcb70483a8db Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 12:14:46 +0200
Subject: [PATCH 258/359] prettier and restoring kallisto/index

---
 modules.json                                   | 2 +-
 modules/nf-core/modules/kallisto/index/main.nf | 2 +-
 nextflow_schema.json                           | 4 ++--
 3 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules.json b/modules.json
index ac19fc71..372809bf 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/modules/kallisto/index/main.nf b/modules/nf-core/modules/kallisto/index/main.nf
index 4136486b..0f10e564 100644
--- a/modules/nf-core/modules/kallisto/index/main.nf
+++ b/modules/nf-core/modules/kallisto/index/main.nf
@@ -5,7 +5,7 @@ process KALLISTO_INDEX {
     conda (params.enable_conda ? "bioconda::kallisto=0.46.2" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/kallisto:0.46.2--h4f7b962_1' :
-        'quay.io/biocontainers/kallisto:0.48.0--h0d531b0_1' }"
+        'quay.io/biocontainers/kallisto:0.46.2--h4f7b962_1' }"
 
     input:
     path fasta
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 17f23e1b..d5b0a54a 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -236,7 +236,7 @@
                     "type": "string",
                     "format": "file-path",
                     "mimetype": "text/plain",
-                    "pattern":  "^\\S+\\.fn?a(sta)?(\\.gz)?$",
+                    "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",
                     "description": "Path to GTF genome file.",
                     "fa_icon": "far fa-file-code"
                 },
@@ -244,7 +244,7 @@
                     "type": "string",
                     "format": "file-path",
                     "mimetype": "text/plain",
-                    "pattern":  "^\\S+\\.refflat?$",
+                    "pattern": "^\\S+\\.refflat?$",
                     "description": "Path to GTF genome file.",
                     "fa_icon": "far fa-file-code"
                 }

From 7b1e8d78bc3d90d2639678980c28e31120bb4bc5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 13:39:12 +0200
Subject: [PATCH 259/359] add ci test arriba references

---
 .github/workflows/ci.yml | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 791ad5a0..a92b7bcd 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -42,11 +42,11 @@ jobs:
           wget -qO- get.nextflow.io | bash
           sudo mv nextflow /usr/local/bin/
 
-      - name: Run pipeline with test data
+      - name: Test building arriba references
         # TODO nf-core: You can customise CI pipeline run tests as required
         # For example: adding multiple test runs with different parameters
         # Remember that you can parallelise this by using strategy.matrix
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results --arriba --build_references
 
 #

From 365d72fae70bd0d598f22ed102182d882191e002 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 14:26:32 +0200
Subject: [PATCH 260/359] update parameter input

---
 nextflow.config                       |  6 +++---
 subworkflows/local/arriba_workflow.nf | 12 +++---------
 2 files changed, 6 insertions(+), 12 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 243fff5c..b33f1ee6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -35,9 +35,8 @@ params {
 
     // Alignment options
     star_ignore_sjdbgtf        = false
-    seq_center                 = null
-    seq_platform               = null
-    star_index                 = null
+    seq_center                 = false
+    seq_platform               = false
 
     // Enable or disable tools
     all                        = false
@@ -48,6 +47,7 @@ params {
     starindex                  = false
     starfusion                 = false
     fusionreport               = false
+    star_index                 = false
 
     // Skip steps
     skip_qc                    = false
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 5a15d30b..fb398ccb 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -10,20 +10,14 @@ include { STAR_ALIGN as STAR_FOR_ARRIBA }               from '../../modules/nf-c
 workflow ARRIBA_WORKFLOW {
     take:
         reads
-        fasta
-        index
 
     main:
         ch_versions = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
         if (params.arriba || params.all) {
-            gtf ="${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-            star_ignore_sjdbgtf = false
-            seq_platform = false
-            seq_center = false
 
-            STAR_FOR_ARRIBA( reads, index, params.gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
+            STAR_FOR_ARRIBA( reads, params.starindex_ref, params.gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
 
             SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam)
@@ -38,7 +32,7 @@ workflow ARRIBA_WORKFLOW {
                 ch_arriba_fusions = params.arriba_fusions
                 ch_arriba_fusion_fail = ch_dummy_file
             } else {
-                ARRIBA ( STAR_FOR_ARRIBA.out.bam, fasta, gtf )
+                ARRIBA ( STAR_FOR_ARRIBA.out.bam, params.fasta, params.gtf )
                 ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
                 GET_PATH_ARRIBA(ARRIBA.out.fusions)
@@ -48,7 +42,7 @@ workflow ARRIBA_WORKFLOW {
                 ch_arriba_fusion_fail = GET_PATH_ARRIBA_FAIL.out.file
             }
 
-            ARRIBA_VISUALISATION(bam_indexed, ch_arriba_fusions, params.arriba_ref, gtf)
+            ARRIBA_VISUALISATION(bam_indexed, ch_arriba_fusions, params.arriba_ref, params.gtf)
             ch_versions = ch_versions.mix(ARRIBA_VISUALISATION.out.versions)
 
             ch_arriba_visualisation = ARRIBA_VISUALISATION.out.pdf

From 5860a614ada1f3c60eb3968c97ea23b9b2cd1201 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 1 Apr 2022 14:29:29 +0200
Subject: [PATCH 261/359] update parameter input for arriba

---
 workflows/rnafusion.nf | 2 --
 1 file changed, 2 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 6d3aa53a..05dac0f0 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -140,8 +140,6 @@ workflow RNAFUSION {
     // Run STAR alignment and Arriba
     ARRIBA_WORKFLOW (
         ch_cat_fastq,
-        params.fasta,
-        params.starindex_ref,
     )
     ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions.first().ifEmpty(null))
 

From f7984833a4ef10c79b7c45ad17aa74c1fe141f66 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 4 Apr 2022 16:11:10 +0200
Subject: [PATCH 262/359] move fasta param in nexflow.config

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index b33f1ee6..d88031c6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -11,7 +11,6 @@ params {
 
     // Input options
     input                      = "fake_input_to_build_refs.csv"
-    fasta                      = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"
     build_references           = false
     cosmic_username            = null
     cosmic_passwd              = null
@@ -27,6 +26,7 @@ params {
     ensembl_version            = 105
     read_length                = 100
     genomes                    = [:]
+    fasta                      = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"
     gtf                        = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf"
     chrgtf                     = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf"
     transcript                 = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.cdna.all.fa.gz"

From b611045fb72a1f069fbb8e5bffdfaae1d1252515 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 4 Apr 2022 16:45:23 +0200
Subject: [PATCH 263/359] star_index to starindex

---
 nextflow.config | 1 -
 1 file changed, 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index d88031c6..f5b72851 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -47,7 +47,6 @@ params {
     starindex                  = false
     starfusion                 = false
     fusionreport               = false
-    star_index                 = false
 
     // Skip steps
     skip_qc                    = false

From f123982e067347db83439836d034546a93a5aaf0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 4 Apr 2022 16:47:52 +0200
Subject: [PATCH 264/359] star_index to starindex

---
 nextflow_schema.json | 7 -------
 1 file changed, 7 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 78a5a284..d80deea6 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -443,13 +443,6 @@
                     "hidden": true,
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "Setting false will use GTF file for STAR alignment"
-                },
-                "star_index": {
-                    "type": "string",
-                    "description": "Path of STAR index directory",
-                    "hidden": true,
-                    "fa_icon": "fas fa-toolbox",
-                    "help_text": "Provide the index directory for the STAR alignment"
                 }
             }
         }

From cfba1d016349fcec5506f6b316f21693362b11b9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 4 Apr 2022 16:48:40 +0200
Subject: [PATCH 265/359] check if basic references exist before executing:
 should throw an error with relative paths

---
 workflows/rnafusion.nf | 8 ++++++++
 1 file changed, 8 insertions(+)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 05dac0f0..ffc7d4fa 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -13,6 +13,14 @@ WorkflowRnafusion.initialise(params, log)
 
 if (file(params.input).exists() || params.build_references) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet does not exist or was not specified!' }
 
+def checkPathParamList = [
+    params.multiqc_config, params.fasta,
+    params.gtf, params.chrgtf, params.transcript
+]
+
+for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
+
+
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     CONFIG FILES

From 2c0cbfbcbabfffbfbe561f78a617e15fb6011f6f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 4 Apr 2022 18:27:40 +0200
Subject: [PATCH 266/359] add check for absolute paths

---
 workflows/rnafusion.nf | 14 +++++++++++---
 1 file changed, 11 insertions(+), 3 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index ffc7d4fa..54fc18fd 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -14,11 +14,19 @@ WorkflowRnafusion.initialise(params, log)
 if (file(params.input).exists() || params.build_references) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet does not exist or was not specified!' }
 
 def checkPathParamList = [
-    params.multiqc_config, params.fasta,
-    params.gtf, params.chrgtf, params.transcript
+    params.fasta,
+    params.gtf,
+    params.chrgtf,
+    params.transcript
 ]
+for (param in checkPathParamList) if (param) file(param, checkIfExists: true)
+
+
+for (param in checkPathParamList) {
+    if (!file(param).exists()) { exit 1, 'Check that references were built (use --build_references)' }
+    if (!(param == file(param))) { exit 1, 'Check that ABSOLUTE PATHS are used' }
+}
 
-for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
 
 
 /*

From b3d78a886efaa0942a60f4825a775d06edee0861 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 5 Apr 2022 10:06:13 +0200
Subject: [PATCH 267/359] add pizzly ci

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index a92b7bcd..9a317eaf 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -47,6 +47,6 @@ jobs:
         # For example: adding multiple test runs with different parameters
         # Remember that you can parallelise this by using strategy.matrix
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results --arriba --build_references
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results --pizzly --build_references
 
 #

From f4a8a1a66fb7db1d471e055efb48fa99902f67ee Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 5 Apr 2022 10:10:07 +0200
Subject: [PATCH 268/359] update check param

---
 workflows/rnafusion.nf | 2 --
 1 file changed, 2 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 54fc18fd..9183a3b2 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -19,8 +19,6 @@ def checkPathParamList = [
     params.chrgtf,
     params.transcript
 ]
-for (param in checkPathParamList) if (param) file(param, checkIfExists: true)
-
 
 for (param in checkPathParamList) {
     if (!file(param).exists()) { exit 1, 'Check that references were built (use --build_references)' }

From 9fa48812636b9fe6615b3b67fe29fe24e575c9ad Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 5 Apr 2022 10:13:03 +0200
Subject: [PATCH 269/359] update test path

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 9a317eaf..ae896a09 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -47,6 +47,6 @@ jobs:
         # For example: adding multiple test runs with different parameters
         # Remember that you can parallelise this by using strategy.matrix
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results --pizzly --build_references
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly --build_references
 
 #

From 49117d10eb6fcf4fe4f3c5b8eacba7bf0d3b7c49 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 5 Apr 2022 10:17:44 +0200
Subject: [PATCH 270/359] add genomes_base as argument for ci test

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index ae896a09..9cac8416 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -47,6 +47,6 @@ jobs:
         # For example: adding multiple test runs with different parameters
         # Remember that you can parallelise this by using strategy.matrix
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly --build_references
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
 
 #

From 11965472b09c3ba54a19f3b5b004845b692926a3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 12:29:24 +0200
Subject: [PATCH 271/359] add build in awsfulltest

---
 .github/workflows/awsfulltest.yml | 18 ++++++++++++++++++
 1 file changed, 18 insertions(+)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 9b4362ea..5c7e2e38 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -13,6 +13,24 @@ jobs:
     if: github.repository == 'nf-core/rnafusion'
     runs-on: ubuntu-latest
     steps:
+      - name: Launch build workflow via tower
+        uses: nf-core/tower-action@v3
+        # TODO nf-core: You can customise AWS full pipeline tests as required
+        # Add full size test data (but still relatively small datasets for few samples)
+        # on the `test_full_build.config` test runs with only one set of parameters
+        with:
+          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
+          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
+          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
+          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
+          parameters: |
+            {
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
+            }
+          profiles: test_full_build,aws_tower
+          nextflow_config: |
+            process.errorStrategy = 'retry'
+            process.maxRetries = 3
       - name: Launch workflow via tower
         uses: nf-core/tower-action@v3
         # TODO nf-core: You can customise AWS full pipeline tests as required

From 6cdcb397eaf65b4e678794132c14c0e74fa18a91 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 12:36:24 +0200
Subject: [PATCH 272/359] pizzly test build and detect pizzly

---
 .github/workflows/ci.yml | 15 +++++++++++++--
 1 file changed, 13 insertions(+), 2 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 9cac8416..c40da4cb 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -42,11 +42,22 @@ jobs:
           wget -qO- get.nextflow.io | bash
           sudo mv nextflow /usr/local/bin/
 
-      - name: Test building arriba references
+      - name: Test building pizzly references
         # TODO nf-core: You can customise CI pipeline run tests as required
         # For example: adding multiple test runs with different parameters
         # Remember that you can parallelise this by using strategy.matrix
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
+          --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly \
+          --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
+
+      - name: Test building pizzly references
+        # TODO nf-core: You can customise CI pipeline run tests as required
+        # For example: adding multiple test runs with different parameters
+        # Remember that you can parallelise this by using strategy.matrix
+        run: |
+          nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
+          --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly \
+          --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
 
 #

From bf7af328840b69919d3633df437962187a7e2985 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 12:52:10 +0200
Subject: [PATCH 273/359] add nf-core/test-dataset rnafusion reads samplesheet

---
 assets/samplesheet.csv | 5 ++---
 1 file changed, 2 insertions(+), 3 deletions(-)

diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv
index 5f653ab7..4f9acc40 100644
--- a/assets/samplesheet.csv
+++ b/assets/samplesheet.csv
@@ -1,3 +1,2 @@
-sample,fastq_1,fastq_2
-SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz
-SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz,
+sample,fastq_1,fastq_2,strandedness
+test_rnafusion,https://github.com/nf-core/test-datasets/raw/d6cd12c9a69c148ef986d156d110f741df482b04/testdata/human/reads_1.fq.gz,https://github.com/nf-core/test-datasets/raw/d6cd12c9a69c148ef986d156d110f741df482b04/testdata/human/reads_2.fq.gz,forward

From 5eea06f9c23a9689ab4fc4f57979338b30b63229 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 12:53:24 +0200
Subject: [PATCH 274/359] modify genomes.config

---
 conf/genomes.config | 9 +++++----
 1 file changed, 5 insertions(+), 4 deletions(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index c7b30bc0..845445a2 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -11,10 +11,11 @@
 params {
     genomes {
         'GRCh38' {
-            fasta                         = "${params.genomes_base}/ensembl/Homo_sapiens.GRCh38.${params.ensembl_version}.all.fa"
-            gtf                           = "${params.genomes_base}/ensembl/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf"
-            chrgtf                        = "${params.genomes_base}/ensembl/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf"
-            transcript                    = "${params.genomes_base}/ensembl/Homo_sapiens.GRCh38.${params.ensembl_version}.cdna.all.fa.gz"
+            fasta        = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"
+            gtf          = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf"
+            chrgtf       = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf"
+            transcript   = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.cdna.all.fa.gz"
+            refflat      = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf.refflat"
         }
     }
 }

From 69ea09d02c82d726145085c3c6da241cd393ccc8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 12:53:55 +0200
Subject: [PATCH 275/359] qc steps run also for --all

---
 conf/modules.config | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 94ebfafc..75dba207 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -128,12 +128,12 @@ process {
     }
 
     withName: PICARD_COLLECTRNASEQMETRICS {
-        ext.when         = { !params.skip_qc && params.starfusion }
+        ext.when         = { !params.skip_qc && (params.starfusion || params.all) }
 
     }
 
     withName: PICARD_MARKDUPLICATES {
-        ext.when         = { !params.skip_qc && params.starfusion }
+        ext.when         = { !params.skip_qc && (params.starfusion || params.all) }
     }
 
     withName: PIZZLY {
@@ -146,7 +146,7 @@ process {
     }
 
     withName: QUALIMAP_RNASEQ {
-        ext.when         = { !params.skip_qc && params.starfusion }
+        ext.when         = { !params.skip_qc && (params.starfusion || params.all)}
     }
 
     withName: SAMPLESHEET_CHECK {
@@ -166,7 +166,7 @@ process {
     }
 
     withName: SAMTOOLS_INDEX_FOR_QC {
-        ext.when         = { !params.skip_qc && params.starfusion }
+        ext.when         = { !params.skip_qc && (params.starfusion || params.all)}
         publishDir = [
             path: { "${params.outdir}/samtools_index_for_qc" },
             mode: params.publish_dir_mode,

From 6bde30c2a18c846fc46af59b216316af08a372eb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 12:54:47 +0200
Subject: [PATCH 276/359] make test.config simpler and set the different
 options in ci.yml

---
 conf/test.config | 11 -----------
 1 file changed, 11 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 4f1200b8..c222f219 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -21,16 +21,5 @@ params {
 
     // Input data
     input            = 'assets/samplesheet_valid.csv'
-    build_references = true
-    arriba           = true
-    genomes_base     = './results/references'
 
-    // Genome references
-    genome              = 'GRCh38'
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
-    fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
-    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf'
-
-    //STAR index
-    // star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
 }

From cd31b8b3fff62267eebd7c6e6e5a586003a18fdb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 12:57:33 +0200
Subject: [PATCH 277/359] use getGenomeAttribute for parameters in
 genomes.config only

---
 conf/test_full.config | 19 +++----------------
 main.nf               |  8 +++++---
 2 files changed, 8 insertions(+), 19 deletions(-)

diff --git a/conf/test_full.config b/conf/test_full.config
index 76e9bb20..6309067d 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -14,23 +14,10 @@ params {
     config_profile_name        = 'Full test profile'
     config_profile_description = 'Full test dataset to check pipeline function'
 
-    // Limit resources so that this can run on GitHub Actions
-    max_cpus   = 2
-    max_memory = 6.GB
-    max_time   = 6.h
-
     // Input data
     genome                 = 'GRCh38'
-    input                  = 'assets/samplesheet_valid.csv'
-    build_references       = true
-    arriba                 = true
-    genomes_base           = './results/references'
-
-    // Genome references
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta'
-    fasta_fai           = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai'
-    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf'
+    input                  = 'assets/samplesheet.csv'
+    genomes_base           = "${params.outdir}/references"
+    all                    = true
 
-    //STAR index
-    // star_index          = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz'
 }
diff --git a/main.nf b/main.nf
index e164b8fe..2408022e 100644
--- a/main.nf
+++ b/main.nf
@@ -17,8 +17,11 @@ nextflow.enable.dsl = 2
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-params.genomes_base      = WorkflowMain.getGenomeAttribute(params, 'genomes_base')
-
+params.fasta           = WorkflowMain.getGenomeAttribute(params, 'fasta')
+params.gtf             = WorkflowMain.getGenomeAttribute(params, 'gtf')
+params.chrgtf          = WorkflowMain.getGenomeAttribute(params, 'chrgtf')
+params.transcript      = WorkflowMain.getGenomeAttribute(params, 'transcript')
+params.refflat         = WorkflowMain.getGenomeAttribute(params, 'refflat')
 
 /*
 ========================================================================================
@@ -26,7 +29,6 @@ params.genomes_base      = WorkflowMain.getGenomeAttribute(params, 'genomes_base
 ========================================================================================
 */
 
-params.build_references  = WorkflowMain.getGenomeAttribute(params, 'build_references')
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From 7929280ebe19011ed34219c6982aa9145bac8438 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 12:59:11 +0200
Subject: [PATCH 278/359] update nf-core modules

---
 modules.json                                        | 12 ++++++------
 modules/nf-core/modules/samtools/faidx/main.nf      |  6 +++---
 modules/nf-core/modules/samtools/index/main.nf      |  6 +++---
 modules/nf-core/modules/samtools/view/main.nf       |  6 +++---
 modules/nf-core/modules/star/genomegenerate/main.nf |  6 +++---
 5 files changed, 18 insertions(+), 18 deletions(-)

diff --git a/modules.json b/modules.json
index 372809bf..ff9da3c1 100644
--- a/modules.json
+++ b/modules.json
@@ -34,23 +34,23 @@
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
             },
             "samtools/faidx": {
-                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
+                "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773"
             },
             "samtools/index": {
-                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
+                "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773"
             },
             "samtools/sort": {
-                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
+                "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773"
             },
             "samtools/view": {
-                "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35"
+                "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773"
             },
             "star/align": {
                 "git_sha": "1dddf1ce9443e3d93853d86e7a7aab52e5b4d614"
             },
             "star/genomegenerate": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+                "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773"
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf
index 053279ff..fdce7d9b 100644
--- a/modules/nf-core/modules/samtools/faidx/main.nf
+++ b/modules/nf-core/modules/samtools/faidx/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_FAIDX {
     tag "$fasta"
     label 'process_low'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.15" : null)
+    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' :
-        'quay.io/biocontainers/samtools:1.15--h1170115_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
+        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf
index fff6e1b8..e04e63e8 100644
--- a/modules/nf-core/modules/samtools/index/main.nf
+++ b/modules/nf-core/modules/samtools/index/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_INDEX {
     tag "$meta.id"
     label 'process_low'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.15" : null)
+    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' :
-        'quay.io/biocontainers/samtools:1.15--h1170115_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
+        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
 
     input:
     tuple val(meta), path(input)
diff --git a/modules/nf-core/modules/samtools/view/main.nf b/modules/nf-core/modules/samtools/view/main.nf
index 75aad063..5f14fbbf 100644
--- a/modules/nf-core/modules/samtools/view/main.nf
+++ b/modules/nf-core/modules/samtools/view/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_VIEW {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.15" : null)
+    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' :
-        'quay.io/biocontainers/samtools:1.15--h1170115_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
+        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
 
     input:
     tuple val(meta), path(input)
diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf
index 50d280dd..e5568f1d 100644
--- a/modules/nf-core/modules/star/genomegenerate/main.nf
+++ b/modules/nf-core/modules/star/genomegenerate/main.nf
@@ -3,10 +3,10 @@ process STAR_GENOMEGENERATE {
     label 'process_high'
 
     // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.13 conda-forge::gawk=5.1.0" : null)
+    conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.15.1 conda-forge::gawk=5.1.0" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0' :
-        'quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0' }"
+        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:1c4c32d87798d425c970ececfbadd155e7560277-0' :
+        'quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:1c4c32d87798d425c970ececfbadd155e7560277-0' }"
 
     input:
     path fasta

From a284dce87f5a93f7007d202dbe580b1602058be6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 13:05:53 +0200
Subject: [PATCH 279/359] single end from param to meta

---
 modules/local/fusioninspector/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index cd575734..8f2df0ac 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -23,7 +23,7 @@ process FUSIONINSPECTOR {
 
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def fasta = params.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
+    def fasta = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
     def args = task.ext.args ?: ''
     """
     FusionInspector \\

From 7a85626b6d3658bf2239b0f0028e2dd425abe393 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 13:06:14 +0200
Subject: [PATCH 280/359] add tool also when using --all

---
 modules/local/fusionreport/detect/main.nf | 10 +++++-----
 1 file changed, 5 insertions(+), 5 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index e1a12056..badf5937 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -28,11 +28,11 @@ process FUSIONREPORT {
     task.ext.when == null || task.ext.when
 
     script:
-    def tools = params.arriba         ? "--arriba ${arriba_fusions} " : ''
-    tools    += params.pizzly         ? "--pizzly ${pizzly_fusions} " : ''
-    tools    += params.squid          ? "--squid ${squid_fusions} " : ''
-    tools    += params.starfusion     ? "--starfusion ${starfusion_fusions} " : ''
-    tools    += params.fusioncatcher  ? "--fusioncatcher ${fusioncatcher_fusions} " : ''
+    def tools = params.arriba || params.all         ? "--arriba ${arriba_fusions} " : ''
+    tools    += params.pizzly || params.all         ? "--pizzly ${pizzly_fusions} " : ''
+    tools    += params.squid  || params.all         ? "--squid ${squid_fusions} " : ''
+    tools    += params.starfusion  || params.all    ? "--starfusion ${starfusion_fusions} " : ''
+    tools    += params.fusioncatcher  || params.all ? "--fusioncatcher ${fusioncatcher_fusions} " : ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     fusion_report run $meta.id . $fusionreport_ref $tools --allow-multiple-gene-symbols

From 2f6e6b52c552a4c932651f78bcc5064a6d1ee391 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 13:06:32 +0200
Subject: [PATCH 281/359] update kallisto meta

---
 modules/local/kallisto/quant/meta.yml | 16 +++++++++++++---
 1 file changed, 13 insertions(+), 3 deletions(-)

diff --git a/modules/local/kallisto/quant/meta.yml b/modules/local/kallisto/quant/meta.yml
index eef0a2b0..31821aa6 100644
--- a/modules/local/kallisto/quant/meta.yml
+++ b/modules/local/kallisto/quant/meta.yml
@@ -11,16 +11,26 @@ tools:
       licence: ["BSD-2-Clause"]
 
 input:
-  - fasta:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
       type: file
-      description: genome fasta file
-      pattern: "*.{fasta*}"
+      description: FASTQ file
+      pattern: "*.{fastq}"
   - reference:
       type: directory
       description: Path to kallisto index
       pattern: "*"
 
 output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
   - versions:
       type: file
       description: File containing software versions

From 439e1aa6648f046be61b15ca0b5c972976e48358 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 13:06:50 +0200
Subject: [PATCH 282/359] single end from param to meta

---
 modules/local/starfusion/detect/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 851e400d..cb69f01e 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -21,7 +21,7 @@ process STARFUSION {
 
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def fasta = params.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
+    def fasta = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
     def args = task.ext.args ?: ''
     """
     STAR-Fusion \\

From ef624f6764848b4b045682da1998944b0aefe14a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 13:44:50 +0200
Subject: [PATCH 283/359] add test full build and replace genomes.config
 parameters with absolute paths

---
 conf/test_full_build.config               | 25 +++++++++++++++++++++++
 subworkflows/local/arriba_workflow.nf     |  8 +++++---
 subworkflows/local/pizzly_workflow.nf     |  4 +++-
 subworkflows/local/qc_workflow.nf         |  6 ++++--
 subworkflows/local/squid_workflow.nf      |  7 ++++---
 subworkflows/local/starfusion_workflow.nf |  3 ++-
 6 files changed, 43 insertions(+), 10 deletions(-)
 create mode 100644 conf/test_full_build.config

diff --git a/conf/test_full_build.config b/conf/test_full_build.config
new file mode 100644
index 00000000..6243a1e1
--- /dev/null
+++ b/conf/test_full_build.config
@@ -0,0 +1,25 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for running full-size tests
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines input files and everything required to run a full size pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/rnafusion -profile test_full_build,<docker/singularity> --outdir <OUTDIR>
+
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    config_profile_name        = 'Full build test profile'
+    config_profile_description = 'Full build test dataset to check pipeline function'
+
+
+    // Input data
+    genome                 = 'GRCh38'
+    build_references       = true
+    all                    = true
+    genomes_base           = "${params.outdir}/references"
+    cosmic_username        = "${{ secrets.cosmic_username }}"
+    cosmic_passwd          = "${{ secrets.cosmic_passwd }}"
+}
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index fb398ccb..f905ff09 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -10,6 +10,8 @@ include { STAR_ALIGN as STAR_FOR_ARRIBA }               from '../../modules/nf-c
 workflow ARRIBA_WORKFLOW {
     take:
         reads
+        ch_gtf
+        ch_fasta
 
     main:
         ch_versions = Channel.empty()
@@ -17,7 +19,7 @@ workflow ARRIBA_WORKFLOW {
 
         if (params.arriba || params.all) {
 
-            STAR_FOR_ARRIBA( reads, params.starindex_ref, params.gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
+            STAR_FOR_ARRIBA( reads, params.starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
 
             SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam)
@@ -32,7 +34,7 @@ workflow ARRIBA_WORKFLOW {
                 ch_arriba_fusions = params.arriba_fusions
                 ch_arriba_fusion_fail = ch_dummy_file
             } else {
-                ARRIBA ( STAR_FOR_ARRIBA.out.bam, params.fasta, params.gtf )
+                ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf )
                 ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
                 GET_PATH_ARRIBA(ARRIBA.out.fusions)
@@ -42,7 +44,7 @@ workflow ARRIBA_WORKFLOW {
                 ch_arriba_fusion_fail = GET_PATH_ARRIBA_FAIL.out.file
             }
 
-            ARRIBA_VISUALISATION(bam_indexed, ch_arriba_fusions, params.arriba_ref, params.gtf)
+            ARRIBA_VISUALISATION(bam_indexed, ch_arriba_fusions, params.arriba_ref, ch_gtf)
             ch_versions = ch_versions.mix(ARRIBA_VISUALISATION.out.versions)
 
             ch_arriba_visualisation = ARRIBA_VISUALISATION.out.pdf
diff --git a/subworkflows/local/pizzly_workflow.nf b/subworkflows/local/pizzly_workflow.nf
index 2c26aca9..7ef281e4 100644
--- a/subworkflows/local/pizzly_workflow.nf
+++ b/subworkflows/local/pizzly_workflow.nf
@@ -6,6 +6,8 @@ include { GET_PATH          }     from '../../modules/local/getpath/main'
 workflow PIZZLY_WORKFLOW {
     take:
         reads
+        ch_gtf
+        ch_transcript
 
     main:
         ch_versions = Channel.empty()
@@ -19,7 +21,7 @@ workflow PIZZLY_WORKFLOW {
                 KALLISTO_QUANT( reads, params.pizzly_ref )
                 ch_versions = ch_versions.mix(KALLISTO_QUANT.out.versions)
 
-                PIZZLY( KALLISTO_QUANT.out.txt, params.transcript, params.gtf )
+                PIZZLY( KALLISTO_QUANT.out.txt, ch_transcript, ch_gtf )
                 ch_versions = ch_versions.mix(PIZZLY.out.versions)
 
                 GET_PATH(PIZZLY.out.fusions)
diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index c55c3821..468de3a9 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -10,12 +10,14 @@ include { PICARD_MARKDUPLICATES }                      from '../../modules/nf-co
 workflow QC_WORKFLOW {
     take:
         bam_sorted
+        ch_gtf
+        ch_refflat
 
     main:
         ch_versions = Channel.empty()
         ch_qualimap_qc = Channel.empty()
 
-        QUALIMAP_RNASEQ(bam_sorted, params.gtf)
+        QUALIMAP_RNASEQ(bam_sorted, ch_gtf)
         ch_versions = ch_versions.mix(QUALIMAP_RNASEQ.out.versions)
         ch_qualimap_qc = QUALIMAP_RNASEQ.out.results.ifEmpty(null)
 
@@ -24,7 +26,7 @@ workflow QC_WORKFLOW {
 
         bam_indexed = bam_sorted.join(SAMTOOLS_INDEX_FOR_QC.out.bai)
 
-        PICARD_COLLECTRNASEQMETRICS(bam_indexed, params.refflat, [])
+        PICARD_COLLECTRNASEQMETRICS(bam_indexed, ch_refflat, [])
         ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
 
         PICARD_MARKDUPLICATES(bam_sorted)
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 5de318ff..b8e4ebd7 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -6,9 +6,10 @@ include { SQUID_ANNOTATE }                              from '../../modules/loca
 include { STAR_ALIGN as STAR_FOR_SQUID }                from '../../modules/nf-core/modules/star/align/main'
 
 workflow SQUID_WORKFLOW {
+
     take:
         reads
-        fast
+        ch_gtf
 
     main:
         ch_versions = Channel.empty()
@@ -19,7 +20,7 @@ workflow SQUID_WORKFLOW {
                 ch_squid_fusions = params.squid_fusions
             } else {
 
-            STAR_FOR_SQUID( reads, params.starindex_ref, params.gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
+            STAR_FOR_SQUID( reads, params.starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
             SAMTOOLS_VIEW_FOR_SQUID ( STAR_FOR_SQUID.out.sam, [] )
@@ -33,7 +34,7 @@ workflow SQUID_WORKFLOW {
             SQUID ( bam_sorted )
             ch_versions = ch_versions.mix(SQUID.out.versions)
 
-            SQUID_ANNOTATE ( SQUID.out.fusions, params.gtf )
+            SQUID_ANNOTATE ( SQUID.out.fusions, ch_gtf )
             ch_versions = ch_versions.mix(SQUID_ANNOTATE.out.versions)
 
             GET_PATH(SQUID_ANNOTATE.out.fusions_annotated)
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index bfb81c0f..f7996205 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -6,6 +6,7 @@ include { GET_PATH }                             from '../../modules/local/getpa
 workflow STARFUSION_WORKFLOW {
     take:
         reads
+        ch_chrgtf
 
     main:
         ch_versions = Channel.empty()
@@ -17,7 +18,7 @@ workflow STARFUSION_WORKFLOW {
                 ch_starfusion_fusions = params.starfusion_fusions
             } else {
 
-                STAR_FOR_STARFUSION( reads, params.starindex_ref, params.chrgtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
+                STAR_FOR_STARFUSION( reads, params.starindex_ref, ch_chrgtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
                 ch_align = STAR_FOR_STARFUSION.out.bam_sorted
 

From 7de86c0202dcf6c51fe909adf5f8637673775a76 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 13:46:08 +0200
Subject: [PATCH 284/359] replace genomes.config parameters with absolute paths
 and add check to reject squid+ensembl 105 and relative paths

---
 workflows/rnafusion.nf | 80 +++++++++++++++++++++++++-----------------
 1 file changed, 48 insertions(+), 32 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 9183a3b2..6c5a9e43 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -13,19 +13,25 @@ WorkflowRnafusion.initialise(params, log)
 
 if (file(params.input).exists() || params.build_references) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet does not exist or was not specified!' }
 
+
+
 def checkPathParamList = [
     params.fasta,
     params.gtf,
     params.chrgtf,
-    params.transcript
+    params.transcript,
+    params.refflat
 ]
 
-for (param in checkPathParamList) {
-    if (!file(param).exists()) { exit 1, 'Check that references were built (use --build_references)' }
-    if (!(param == file(param))) { exit 1, 'Check that ABSOLUTE PATHS are used' }
-}
-
+for (param in checkPathParamList) if ((param) && !params.build_references) file(param, checkIfExists: true)
+for (param in checkPathParamList) if ((param)!= file(param).toString() && !params.build_references) { exit 1, 'ABSOLUTE PATHS are required! Check for trailing "/" at the end of paths too.' }
+if ((params.squid || params.all) && params.ensembl_version == 105) { exit 1, 'Ensembl version 105 is not supported by squid' }
 
+ch_fasta = file(params.fasta)
+ch_gtf = file(params.gtf)
+ch_chrgtf = file(params.chrgtf)
+ch_transcript = file(params.transcript)
+ch_refflat = file(params.refflat)
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -125,35 +131,14 @@ workflow RNAFUSION {
     )
     ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
-    CUSTOM_DUMPSOFTWAREVERSIONS (
-        ch_versions.unique().collectFile(name: 'collated_versions.yml')
-    )
-
-    //
-    // MODULE: MultiQC
-    //
-    workflow_summary    = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params)
-    ch_workflow_summary = Channel.value(workflow_summary)
-
-    ch_multiqc_files = Channel.empty()
-    ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
-    ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
-    ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
-    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
-
-    MULTIQC (
-        ch_multiqc_files.collect()
-    )
-
-    multiqc_report       = MULTIQC.out.report.toList()
-    ch_versions          = ch_versions.mix(MULTIQC.out.versions)
 
 
 
     // Run STAR alignment and Arriba
     ARRIBA_WORKFLOW (
         ch_cat_fastq,
+        ch_gtf,
+        ch_fasta
     )
     ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions.first().ifEmpty(null))
 
@@ -161,21 +146,25 @@ workflow RNAFUSION {
 
     PIZZLY_WORKFLOW (
         ch_cat_fastq,
+        ch_gtf,
+        ch_transcript
     )
     ch_versions = ch_versions.mix(PIZZLY_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
 // Run squid
+
     SQUID_WORKFLOW (
         ch_cat_fastq,
-        params.fasta
+        ch_gtf
     )
     ch_versions = ch_versions.mix(SQUID_WORKFLOW.out.versions.first().ifEmpty(null))
 
 
 //Run STAR fusion
     STARFUSION_WORKFLOW (
-        ch_cat_fastq
+        ch_cat_fastq,
+        ch_chrgtf
     )
     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions.first().ifEmpty(null))
 
@@ -210,10 +199,37 @@ workflow RNAFUSION {
 
     //QC
     QC_WORKFLOW (
-        STARFUSION_WORKFLOW.out.bam_sorted
+        STARFUSION_WORKFLOW.out.bam_sorted,
+        ch_gtf,
+        ch_refflat
     )
     ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions.first().ifEmpty(null))
 
+    CUSTOM_DUMPSOFTWAREVERSIONS (
+        ch_versions.unique().collectFile(name: 'collated_versions.yml')
+    )
+
+
+    //
+    // MODULE: MultiQC
+    //
+    workflow_summary    = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params)
+    ch_workflow_summary = Channel.value(workflow_summary)
+
+    ch_multiqc_files = Channel.empty()
+    ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+
+    MULTIQC (
+        ch_multiqc_files.collect()
+    )
+
+    multiqc_report       = MULTIQC.out.report.toList()
+    ch_versions          = ch_versions.mix(MULTIQC.out.versions)
+
 }
 
 

From a6ddef309a36f2060ca70537b03fa2229792dbb5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Apr 2022 13:46:33 +0200
Subject: [PATCH 285/359] fix bug of cosmic username

---
 workflows/build_references.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 1227ed04..7ee272b9 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -52,7 +52,7 @@ workflow BUILD_REFERENCES {
     }
 
     if (params.fusionreport || params.all) {
-        FUSIONREPORT_DOWNLOAD( params.cosmic_usr, params.cosmic_passwd )
+        FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
     }
 
 }

From bd517f82df1307a0cd002a53c02b03d0f21a20ea Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 13:44:39 +0200
Subject: [PATCH 286/359] download ctat lib for starfusion

---
 modules/local/starfusion/download/main.nf | 12 ++++++------
 1 file changed, 6 insertions(+), 6 deletions(-)

diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index efe384fb..88fb1312 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -8,19 +8,19 @@ process STARFUSION_DOWNLOAD {
         container "docker.io/trinityctat/starfusion:1.10.1"
     }
 
-    input:
-    path fasta
-    path gtf
-
     output:
-    path "*"  , emit: reference
+    path "ctat_genome_lib_build_dir/*"            , emit: reference
+    path "ctat_genome_lib_build_dir/ref_annot.gtf", emit: chrgtf
+
 
     script:
     """
-    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --no-check-certificate
+    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.10/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --no-check-certificate
 
     tar xvf GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz
 
     rm GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz
+
+    mv */ctat_genome_lib_build_dir .
     """
 }

From 5ce736c608d17b1717e6a169868308eb30d474db Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 13:45:41 +0200
Subject: [PATCH 287/359] adapt nextflow config and new parameter
 starfusion_build

---
 nextflow.config | 21 +++++++++++----------
 1 file changed, 11 insertions(+), 10 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index f5b72851..53bd7416 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -23,14 +23,11 @@ params {
     // Genome
     genome                     = 'GRCh38'
     genomes_base               = "${params.outdir}/references"
-    ensembl_version            = 105
+    ensembl_version            = 102
     read_length                = 100
     genomes                    = [:]
-    fasta                      = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"
-    gtf                        = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf"
-    chrgtf                     = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf"
-    transcript                 = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.cdna.all.fa.gz"
-    refflat                    = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf.refflat"
+    starfusion_build           = false
+
 
 
     // Alignment options
@@ -58,7 +55,7 @@ params {
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v102"
     pizzly_ref                    = "${params.genomes_base}/pizzly/kallisto"
     squid_ref                     = "${params.genomes_base}/squid"
-    starfusion_ref                = "${params.genomes_base}/starfusion/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play"
+    starfusion_ref                = "${params.genomes_base}/starfusion/ctat_genome_lib_build_dir"
     starindex_ref                 = "${params.genomes_base}/star"
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
 
@@ -71,7 +68,7 @@ params {
     fusioncatcher_fusions         = null
 
     // Boilerplate options
-    outdir                            = './results'
+    outdir                            = null
     tracedir                          = "${params.outdir}/pipeline_info"
     publish_dir_mode                  = 'copy'
     email                             = null
@@ -103,6 +100,8 @@ params {
 // Load base.config by default for all pipelines
 includeConfig 'conf/base.config'
 
+includeConfig 'conf/genomes.config'
+
 // Load nf-core custom profiles from different Institutions
 try {
     includeConfig "${params.custom_config_base}/nfcore_custom.config"
@@ -165,8 +164,10 @@ profiles {
         podman.enabled         = false
         shifter.enabled        = false
     }
-    test      { includeConfig 'conf/test.config'      }
-    test_full { includeConfig 'conf/test_full.config' }
+    test            { includeConfig 'conf/test.config'      }
+    test_full       { includeConfig 'conf/test_full.config' }
+    test_full_build { includeConfig 'conf/test_full_build.config' }
+
 }
 
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container

From 21254cca193678e2e9e15dc2c70ba28e93b2f9db Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 13:49:00 +0200
Subject: [PATCH 288/359] adapt schema

---
 nextflow_schema.json | 7 ++++++-
 1 file changed, 6 insertions(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index d80deea6..8cae433b 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -73,6 +73,11 @@
                     "description": "ensembl version",
                     "default": 105
                 },
+                "starfusion_build":{
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "If set, starfusion references are built from scratch instead of downloaded (default)"
+                },
                 "read_length": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
@@ -229,7 +234,7 @@
                     "type": "string",
                     "format": "file-path",
                     "mimetype": "text/plain",
-                    "pattern": "^\\S+\\.chr.gtf?(\\.gz)?$",
+                    "pattern": "^\\S+\\.gtf?(\\.gz)?$",
                     "description": "Path to GTF genome file.",
                     "fa_icon": "far fa-file-code"
                 },

From be69ab4b0628a4ff057c4846b38156f013f0356c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 13:51:05 +0200
Subject: [PATCH 289/359] add a build starfusion module

---
 modules/local/starfusion/build/main.nf  | 41 +++++++++++++++++++++++++
 modules/local/starfusion/build/meta.yml | 27 ++++++++++++++++
 2 files changed, 68 insertions(+)
 create mode 100644 modules/local/starfusion/build/main.nf
 create mode 100644 modules/local/starfusion/build/meta.yml

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
new file mode 100644
index 00000000..09e787a0
--- /dev/null
+++ b/modules/local/starfusion/build/main.nf
@@ -0,0 +1,41 @@
+process STARFUSION_BUILD {
+    tag 'star-fusion'
+
+    conda (params.enable_conda ? "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.10.0 bioconda::trinity=date.2011_11_2 bioconda::samtools=1.9 bioconda::star=2.7.8a" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "docker.io/trinityctat/starfusion:1.10.1"
+    } else {
+        container "docker.io/trinityctat/starfusion:1.10.1"
+    }
+
+    input:
+    path fasta
+    path gtf
+
+    output:
+    path "*"  , emit: reference
+
+    script:
+    def binPath = ( params.enable_conda ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl" )
+    """
+    export TMPDIR=/tmp
+    wget ftp://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
+    wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz -O CTAT_HumanFusionLib_Mar2021.dat.gz --no-check-certificate
+    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p --no-check-certificate
+    gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
+    $binPath \\
+        --genome_fa $fasta \\
+        --gtf $gtf \\
+        --annot_filter_rule AnnotFilterRule.pm \\
+        --fusion_annot_lib CTAT_HumanFusionLib_Mar2021.dat.gz \\
+        --pfam_db Pfam-A.hmm \\
+        --dfam_db homo_sapiens_dfam.hmm \\
+        --max_readlength $params.read_length \\
+        --CPU $task.cpus
+    """
+}
diff --git a/modules/local/starfusion/build/meta.yml b/modules/local/starfusion/build/meta.yml
new file mode 100644
index 00000000..dc7fee75
--- /dev/null
+++ b/modules/local/starfusion/build/meta.yml
@@ -0,0 +1,27 @@
+name: starfusion_downloadgenome
+description: Download STAR-fusion genome resource required to run STAR-Fusion caller
+keywords:
+  - downoad
+tools:
+  - star-fusion:
+      description: Fusion calling algorithm for RNAseq data
+      homepage: https://github.com/STAR-Fusion/
+      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki/installing-star-fusion
+      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion
+      doi: "10.1186/s13059-019-1842-9"
+      licence: ["GPL v3"]
+
+input:
+  - genome_version:
+      type: value
+      description: Genome version - either GRCh38 or GRCh38
+      pattern: "GRCh3*"
+
+output:
+  - reference:
+      type: directory
+      description: Genome resource path
+      pattern: "star-fusion-genome"
+
+authors:
+  - "@praveenraj2018"

From ca635fdae4474c9ddeeb4ab3d413411db8df169b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 13:51:53 +0200
Subject: [PATCH 290/359] adapt to starfusion build/download workflows

---
 workflows/build_references.nf | 11 +++++++++--
 workflows/rnafusion.nf        | 20 ++++++++++++--------
 2 files changed, 21 insertions(+), 10 deletions(-)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 7ee272b9..ccea93a1 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -8,6 +8,7 @@ include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'
 include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/download/main'
+include { STARFUSION_BUILD }                from '../modules/local/starfusion/build/main'
 include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
 include { GTF_TO_REFFLAT }                  from '../modules/local/uscs/custom_gtftogenepred/main'
 
@@ -47,8 +48,14 @@ workflow BUILD_REFERENCES {
     }
 
     if (params.starfusion || params.all) {
-        GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
-        STARFUSION_DOWNLOAD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
+        if (params.starfusion_build){
+            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
+            GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
+        }
+        else {
+            STARFUSION_DOWNLOAD()
+            GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.chrgtf)
+        }
     }
 
     if (params.fusionreport || params.all) {
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 6c5a9e43..9368244e 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -14,22 +14,23 @@ WorkflowRnafusion.initialise(params, log)
 if (file(params.input).exists() || params.build_references) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet does not exist or was not specified!' }
 
 
+ch_chrgtf = params.starfusion_build ? file(params.chrgtf) : file("${params.starfusion_ref}/ref_annot.gtf")
+ch_starindex_ref = params.starfusion_build ? params.starindex_ref : "${params.starfusion_ref}/ref_genome.fa.star.idx"
 
 def checkPathParamList = [
     params.fasta,
     params.gtf,
-    params.chrgtf,
+    ch_chrgtf,
     params.transcript,
-    params.refflat
+    // params.refflat
 ]
 
 for (param in checkPathParamList) if ((param) && !params.build_references) file(param, checkIfExists: true)
-for (param in checkPathParamList) if ((param)!= file(param).toString() && !params.build_references) { exit 1, 'ABSOLUTE PATHS are required! Check for trailing "/" at the end of paths too.' }
+for (param in checkPathParamList) if ((param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }
 if ((params.squid || params.all) && params.ensembl_version == 105) { exit 1, 'Ensembl version 105 is not supported by squid' }
 
 ch_fasta = file(params.fasta)
 ch_gtf = file(params.gtf)
-ch_chrgtf = file(params.chrgtf)
 ch_transcript = file(params.transcript)
 ch_refflat = file(params.refflat)
 
@@ -138,7 +139,8 @@ workflow RNAFUSION {
     ARRIBA_WORKFLOW (
         ch_cat_fastq,
         ch_gtf,
-        ch_fasta
+        ch_fasta,
+        ch_starindex_ref
     )
     ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions.first().ifEmpty(null))
 
@@ -156,7 +158,8 @@ workflow RNAFUSION {
 
     SQUID_WORKFLOW (
         ch_cat_fastq,
-        ch_gtf
+        ch_gtf,
+        ch_starindex_ref
     )
     ch_versions = ch_versions.mix(SQUID_WORKFLOW.out.versions.first().ifEmpty(null))
 
@@ -164,7 +167,8 @@ workflow RNAFUSION {
 //Run STAR fusion
     STARFUSION_WORKFLOW (
         ch_cat_fastq,
-        ch_chrgtf
+        ch_chrgtf,
+        ch_starindex_ref
     )
     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions.first().ifEmpty(null))
 
@@ -200,7 +204,7 @@ workflow RNAFUSION {
     //QC
     QC_WORKFLOW (
         STARFUSION_WORKFLOW.out.bam_sorted,
-        ch_gtf,
+        ch_chrgtf,
         ch_refflat
     )
     ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions.first().ifEmpty(null))

From 9ffe92f4c7757928767d24dc331f364dd9aa0f95 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 14:00:02 +0200
Subject: [PATCH 291/359] add links to star index and chrgtf from starfusion
 download if starfusion_build=false

---
 subworkflows/local/arriba_workflow.nf     | 3 ++-
 subworkflows/local/qc_workflow.nf         | 4 ++--
 subworkflows/local/squid_workflow.nf      | 3 ++-
 subworkflows/local/starfusion_workflow.nf | 4 ++--
 4 files changed, 8 insertions(+), 6 deletions(-)

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index f905ff09..d468148d 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -12,6 +12,7 @@ workflow ARRIBA_WORKFLOW {
         reads
         ch_gtf
         ch_fasta
+        ch_starindex_ref
 
     main:
         ch_versions = Channel.empty()
@@ -19,7 +20,7 @@ workflow ARRIBA_WORKFLOW {
 
         if (params.arriba || params.all) {
 
-            STAR_FOR_ARRIBA( reads, params.starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
+            STAR_FOR_ARRIBA( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
 
             SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam)
diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index 468de3a9..1258e503 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -10,14 +10,14 @@ include { PICARD_MARKDUPLICATES }                      from '../../modules/nf-co
 workflow QC_WORKFLOW {
     take:
         bam_sorted
-        ch_gtf
+        ch_chrgtf
         ch_refflat
 
     main:
         ch_versions = Channel.empty()
         ch_qualimap_qc = Channel.empty()
 
-        QUALIMAP_RNASEQ(bam_sorted, ch_gtf)
+        QUALIMAP_RNASEQ(bam_sorted, ch_chrgtf)
         ch_versions = ch_versions.mix(QUALIMAP_RNASEQ.out.versions)
         ch_qualimap_qc = QUALIMAP_RNASEQ.out.results.ifEmpty(null)
 
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index b8e4ebd7..1dda8633 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -10,6 +10,7 @@ workflow SQUID_WORKFLOW {
     take:
         reads
         ch_gtf
+        ch_starindex_ref
 
     main:
         ch_versions = Channel.empty()
@@ -20,7 +21,7 @@ workflow SQUID_WORKFLOW {
                 ch_squid_fusions = params.squid_fusions
             } else {
 
-            STAR_FOR_SQUID( reads, params.starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
+            STAR_FOR_SQUID( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
             SAMTOOLS_VIEW_FOR_SQUID ( STAR_FOR_SQUID.out.sam, [] )
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index f7996205..d95e5f81 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -7,6 +7,7 @@ workflow STARFUSION_WORKFLOW {
     take:
         reads
         ch_chrgtf
+        ch_starindex_ref
 
     main:
         ch_versions = Channel.empty()
@@ -17,8 +18,7 @@ workflow STARFUSION_WORKFLOW {
             if (params.starfusion_fusions){
                 ch_starfusion_fusions = params.starfusion_fusions
             } else {
-
-                STAR_FOR_STARFUSION( reads, params.starindex_ref, ch_chrgtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
+                STAR_FOR_STARFUSION( reads, ch_starindex_ref, ch_chrgtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
                 ch_align = STAR_FOR_STARFUSION.out.bam_sorted
 

From 3bb2af9543fa4fd45d0dce53200a7e12c020b64f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 14:02:30 +0200
Subject: [PATCH 292/359] prettier

---
 modules.json         | 2 +-
 nextflow_schema.json | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules.json b/modules.json
index ff9da3c1..86bd0f82 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 8cae433b..674c124a 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -73,7 +73,7 @@
                     "description": "ensembl version",
                     "default": 105
                 },
-                "starfusion_build":{
+                "starfusion_build": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
                     "description": "If set, starfusion references are built from scratch instead of downloaded (default)"

From 1c6d824330a5d1f6d9b3057e5e0cc8dab8d0f95e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 14:06:45 +0200
Subject: [PATCH 293/359] re-install samtools

---
 modules.json                                  | 2 +-
 modules/nf-core/modules/samtools/sort/main.nf | 6 +++---
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules.json b/modules.json
index 86bd0f82..ff9da3c1 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf
index ba46f0c9..b4fc1cbe 100644
--- a/modules/nf-core/modules/samtools/sort/main.nf
+++ b/modules/nf-core/modules/samtools/sort/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_SORT {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.15" : null)
+    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' :
-        'quay.io/biocontainers/samtools:1.15--h1170115_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
+        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
 
     input:
     tuple val(meta), path(bam)

From 1feadbe01a05c602baebbde89777b29e4097864c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 14:08:13 +0200
Subject: [PATCH 294/359] prettier

---
 modules.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules.json b/modules.json
index ff9da3c1..86bd0f82 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}

From 10a601154e28e2a3cfcffcce34312f6c09ee9380 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 15:00:39 +0200
Subject: [PATCH 295/359] address TODOs and update usage for starfusion_build

---
 .github/workflows/awsfulltest.yml |  6 ------
 .github/workflows/ci.yml          |  6 ------
 .nf-core.yml                      |  6 ------
 README.md                         | 33 +++++++++++++++----------------
 conf/modules.config               | 13 +++++++++++-
 docs/usage.md                     | 11 ++++++++++-
 6 files changed, 38 insertions(+), 37 deletions(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 5c7e2e38..6111d6ab 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -15,9 +15,6 @@ jobs:
     steps:
       - name: Launch build workflow via tower
         uses: nf-core/tower-action@v3
-        # TODO nf-core: You can customise AWS full pipeline tests as required
-        # Add full size test data (but still relatively small datasets for few samples)
-        # on the `test_full_build.config` test runs with only one set of parameters
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
@@ -33,9 +30,6 @@ jobs:
             process.maxRetries = 3
       - name: Launch workflow via tower
         uses: nf-core/tower-action@v3
-        # TODO nf-core: You can customise AWS full pipeline tests as required
-        # Add full size test data (but still relatively small datasets for few samples)
-        # on the `test_full.config` test runs with only one set of parameters
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index c40da4cb..79189ac9 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -43,18 +43,12 @@ jobs:
           sudo mv nextflow /usr/local/bin/
 
       - name: Test building pizzly references
-        # TODO nf-core: You can customise CI pipeline run tests as required
-        # For example: adding multiple test runs with different parameters
-        # Remember that you can parallelise this by using strategy.matrix
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
           --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly \
           --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
 
       - name: Test building pizzly references
-        # TODO nf-core: You can customise CI pipeline run tests as required
-        # For example: adding multiple test runs with different parameters
-        # Remember that you can parallelise this by using strategy.matrix
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
           --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly \
diff --git a/.nf-core.yml b/.nf-core.yml
index 7036b988..3805dc81 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,7 +1 @@
 repository_type: pipeline
-lint:
-  files_unchanged:
-    # Added to ignore linting error: actions/setup-node@v1 until fixed release
-    - .github/workflows/linting.yml
-    - .github/workflows/branch.yml
-    - .github/workflows/linting_comment.yml
diff --git a/README.md b/README.md
index 16c62a3d..fcf7c8bc 100644
--- a/README.md
+++ b/README.md
@@ -33,10 +33,10 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 | [Squid](https://github.com/Kingsford-Group/squid)         |        :x:         |  `1.5`   |
 | [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) | :white_check_mark: | `1.10.1` |
 
-<!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
-
 On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).
 
+In rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).
+
 ## Pipeline summary
 
 #### Build references
@@ -56,34 +56,35 @@ On release, automated continuous integration tests run the pipeline on a full-si
 1. Input samplesheet check
 2. Concatenate fastq files per sample
 3. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-4. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
-5. Arriba subworkflow
+4. Arriba subworkflow
    - STAR alignment
    - Samtool sort
    - Samtool index
    - [Arriba](https://github.com/suhrig/arriba) fusion detection
    - [Arriba](https://github.com/suhrig/arriba) visualisation
-6. Pizzly subworkflow
+5. Pizzly subworkflow
    - [Kallisto](https://pachterlab.github.io/kallisto/) quantification
    - [Pizzly](https://github.com/pmelsted/pizzly) fusion detection
-7. Squid subworkflow
+6. Squid subworkflow
    - [STAR](https://github.com/alexdobin/STAR) alignment
    - [Samtools view](http://www.htslib.org/): convert sam output from STAR to bam
    - [Samtools sort](http://www.htslib.org/): bam output from STAR
    - [Squid](https://github.com/Kingsford-Group/squid) fusion detection
    - [Squid](https://github.com/Kingsford-Group/squid) annotate
-8. STAR-fusion subworkflow
+7. STAR-fusion subworkflow
    - [STAR](https://github.com/alexdobin/STAR) alignment
    - [STAR-fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
-9. Fusioncatcher subworkflow
+8. Fusioncatcher subworkflow
    - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
-10. Fusion-report subworkflow
+9. Fusion-report subworkflow
     - Merge all fusions detected by the different tools
     - [Fusion-report](https://github.com/matq007/fusion-report)
-11. FusionInspector subworkflow
+10. FusionInspector subworkflow
     - [FusionInspector](https://github.com/FusionInspector/FusionInspector)
-
-<!-- TODO Add QC steps, MultiQC details -->
+11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
+12. QC for mapped reads ([`QualiMap: BAM QC`](https://kokonech.github.io/qualimap/HG00096.chr20_bamqc/qualimapReport.html))
+13. Index mapped reads ([samtools index`](http://www.htslib.org/))
+14. Collect metrics ([picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)) and ([picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))
 
 ## Quick Start
 
@@ -106,12 +107,12 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 4. Start running your own analysis!
 
-<!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
-
 ```console
-nextflow run nf-core/rnafusion --input samplesheet.csv --outdir <OUTDIR> --genome GRCh38 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
+nextflow run nf-core/rnafusion --input samplesheet.csv --outdir <OUTDIR> --genome GRCh38 --all -profile <docker/singularity/podman/shifter/charliecloud/institute>
 ```
 
+> Note that paths need to be absolute and that runs with conda are not supported.
+
 ## Documentation
 
 The nf-core/rnafusion pipeline comes with documentation about the pipeline [usage](https://nf-co.re/rnafusion/usage), [parameters](https://nf-co.re/rnafusion/parameters) and [output](https://nf-co.re/rnafusion/output).
@@ -132,8 +133,6 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 
 ## Citations
 
-<!-- TODO update zenodo after release -->
-
 If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)
 
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
diff --git a/conf/modules.config b/conf/modules.config
index 75dba207..e9736b92 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -277,7 +277,7 @@ process {
         ]
     }
 
-    withName: STARFUSION_DOWNLOAD {
+    withName: STARFUSION_BUILD {
         cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
         memory = { check_max( 100.GB * task.attempt, 'memory'  ) }
         time   = { check_max( 2.d  * task.attempt, 'time'    ) }
@@ -287,4 +287,15 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
     }
+
+    withName: STARFUSION_DOWNLOAD {
+        cpus   = { check_max( 2    * task.attempt, 'cpus'    ) }
+        memory = { check_max( 24.GB * task.attempt, 'memory'  ) }
+        time   = { check_max( 6.h  * task.attempt, 'time'    ) }
+        publishDir = [
+            path: { "${params.genomes_base}/starfusion" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
+    }
 }
diff --git a/docs/usage.md b/docs/usage.md
index c1820182..6d8d23d1 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -43,6 +43,15 @@ nextflow run nf-core/rnafusion \
 
 This PATH will be the place the references will be saved.
 
+Optional: by default STAR-Fusion references are downloaded. You can also build them from ensembl fasta and gtf files. This allows more flexibility for different organisms but be aware that **this is slow and computationally intensive**:
+
+```bash
+nextflow run nf-core/rnafusion \
+--build_references --starfusion \
+--starfusion_build \
+--genomes_base <PATH>
+```
+
 ### Running the detection tools
 
 ```bash
@@ -55,7 +64,7 @@ Visualisation tools will be run on all fusion detected.
 
 #### Optional manual feed-in of fusion files
 
-It is possible to give the output of each tool manually using the argument: `--<tool>_fusions PATH/TO/FUSION/FILE`.
+It is possible to give the output of each tool manually using the argument: `--<tool>_fusions PATH/TO/FUSION/FILE`: this feature need more testing, don't hesitate to open an issue if you encounter problems.
 
 ## Samplesheet input
 

From 71eac5d7185c6b6c54089725d3469a53f468274d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 15:09:29 +0200
Subject: [PATCH 296/359] update CHANGELOG

---
 CHANGELOG.md    | 5 +++--
 README.md       | 4 ++--
 nextflow.config | 2 +-
 3 files changed, 6 insertions(+), 5 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 515b6801..db763a56 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## [2.0.0] nfcore/rnafusion - 2022/03/??
+## [2.0.0] nfcore/rnafusion - 2022/04/14
 
 Update to DSL2 and newer software/reference versions
 
@@ -15,6 +15,7 @@ Update to DSL2 and newer software/reference versions
 - Added `picard MarkDuplicates v2.26.10` from nf-core modules
 - Added `cat/fastqc` from nf-core modules
 - Added possibility for manually feeding the results of fusions from different tools to speed-up reruns
+- STAR-Fusion references can be downloaded or built (--starfusion_build parameter)
 
 ### Changed
 
@@ -26,7 +27,7 @@ Update to DSL2 and newer software/reference versions
 - Upgrade `fusion-report v2.1.3` to `fusion-report v2.1.5`
 - Upgrade `kallisto v0.44.0` to `kallisto v0.46.2`
 - Upgrade `fastqc v0.11.8` to `fastqc v0.11.9`
-- Upgrade `samtools v1.9` to `samtools v1.15`
+- Upgrade `samtools v1.9` to `samtools v1.15.1`
 - Upgrade `arriba` references from `v1.2.0` to `v2.1.0`
 - Upgrade `fusioncatcher` references from `v98` to `v102`
 - Use `arriba` (detect only), `kallisto` and `STAR` from nf-core modules
diff --git a/README.md b/README.md
index fcf7c8bc..58faab7d 100644
--- a/README.md
+++ b/README.md
@@ -77,8 +77,8 @@ In rnafusion the full-sized test includes reference building and fusion detectio
 8. Fusioncatcher subworkflow
    - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
 9. Fusion-report subworkflow
-    - Merge all fusions detected by the different tools
-    - [Fusion-report](https://github.com/matq007/fusion-report)
+   - Merge all fusions detected by the different tools
+   - [Fusion-report](https://github.com/matq007/fusion-report)
 10. FusionInspector subworkflow
     - [FusionInspector](https://github.com/FusionInspector/FusionInspector)
 11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
diff --git a/nextflow.config b/nextflow.config
index 53bd7416..6b308b00 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -209,7 +209,7 @@ manifest {
     description     = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
     mainScript      = 'main.nf'
     nextflowVersion = '!>=21.10.3'
-    version         = '1.3.0dev'
+    version = '2.0.0'
 }
 
 // Load modules.config for DSL2 module specific options

From c5b3734f1accb23c96f1e6a841cefc5afca88d73 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Apr 2022 16:36:11 +0200
Subject: [PATCH 297/359] inconsistencies with starfusion meta files

---
 CHANGELOG.md                               |  1 +
 modules/local/starfusion/build/meta.yml    | 16 ++++++++++------
 modules/local/starfusion/detect/meta.yml   |  4 ++++
 modules/local/starfusion/download/meta.yml | 12 +++++-------
 4 files changed, 20 insertions(+), 13 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index db763a56..9c6fbda0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -19,6 +19,7 @@ Update to DSL2 and newer software/reference versions
 
 ### Changed
 
+- Upgrade default ensembl version to `105`
 - Upgrade to `nf-core/tools v2.3`
 - Upgrade `Arriba v1.2.0` to `Arriba v2.1.0`
 - Upgrade `FusionCatcher v1.20` to `FusionCatcher v1.33`
diff --git a/modules/local/starfusion/build/meta.yml b/modules/local/starfusion/build/meta.yml
index dc7fee75..c87b251b 100644
--- a/modules/local/starfusion/build/meta.yml
+++ b/modules/local/starfusion/build/meta.yml
@@ -12,16 +12,20 @@ tools:
       licence: ["GPL v3"]
 
 input:
-  - genome_version:
-      type: value
-      description: Genome version - either GRCh38 or GRCh38
-      pattern: "GRCh3*"
+  - fasta:
+      type: file
+      description: genome fasta file
+      pattern: "*.{fasta}"
+  - gtf:
+      type: file
+      description: genome gtf file
+      pattern: "*.{gtf}"
 
 output:
   - reference:
       type: directory
-      description: Genome resource path
-      pattern: "star-fusion-genome"
+      description: Reference dir
+      pattern: "ctat_genome_lib_build_dir"
 
 authors:
   - "@praveenraj2018"
diff --git a/modules/local/starfusion/detect/meta.yml b/modules/local/starfusion/detect/meta.yml
index 136facba..7337dad5 100644
--- a/modules/local/starfusion/detect/meta.yml
+++ b/modules/local/starfusion/detect/meta.yml
@@ -24,6 +24,10 @@ input:
       type: file
       description: Chimeric junction output from STAR aligner
       pattern: "*.{out.junction}"
+  - reference:
+      type: directory
+      description: Reference dir
+      pattern: "ctat_genome_lib_build_dir"
 
 output:
   - meta:
diff --git a/modules/local/starfusion/download/meta.yml b/modules/local/starfusion/download/meta.yml
index dc7fee75..24e84252 100644
--- a/modules/local/starfusion/download/meta.yml
+++ b/modules/local/starfusion/download/meta.yml
@@ -11,17 +11,15 @@ tools:
       doi: "10.1186/s13059-019-1842-9"
       licence: ["GPL v3"]
 
-input:
-  - genome_version:
-      type: value
-      description: Genome version - either GRCh38 or GRCh38
-      pattern: "GRCh3*"
-
 output:
   - reference:
       type: directory
       description: Genome resource path
       pattern: "star-fusion-genome"
+  - gtf:
+      type: file
+      description: genome gtf file
+      pattern: "*.{gtf}"
 
 authors:
-  - "@praveenraj2018"
+  - "@praveenraj2018,@rannick"

From fa19876c0fa87f2decce4f9e517008c70027b696 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 19 Apr 2022 11:23:44 +0200
Subject: [PATCH 298/359] aws full size test for commits to master

---
 .github/workflows/awsfulltest.yml | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 6111d6ab..11f2b82d 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -4,8 +4,8 @@ name: nf-core AWS full size tests
 # It runs the -profile 'test_full' on AWS batch
 
 on:
-  release:
-    types: [published]
+  pull_request_target:
+    branches: [master]
   workflow_dispatch:
 jobs:
   run-tower:

From e0595c5b52b2527396431e82e7b1d4a3fb4983dd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 19 Apr 2022 11:29:26 +0200
Subject: [PATCH 299/359] version to 2.0.0dev and awsfull on publishing

---
 .github/workflows/awsfulltest.yml | 4 ++--
 nextflow.config                   | 2 +-
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 11f2b82d..6111d6ab 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -4,8 +4,8 @@ name: nf-core AWS full size tests
 # It runs the -profile 'test_full' on AWS batch
 
 on:
-  pull_request_target:
-    branches: [master]
+  release:
+    types: [published]
   workflow_dispatch:
 jobs:
   run-tower:
diff --git a/nextflow.config b/nextflow.config
index 6b308b00..061d63b3 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -209,7 +209,7 @@ manifest {
     description     = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
     mainScript      = 'main.nf'
     nextflowVersion = '!>=21.10.3'
-    version = '2.0.0'
+    version = '2.0.0dev'
 }
 
 // Load modules.config for DSL2 module specific options

From e3b9a8e5fcc4f8149f7dadbd4ad0baaac5b88557 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 19 Apr 2022 11:33:25 +0200
Subject: [PATCH 300/359] add aws full test when pushing to master

---
 .github/workflows/awsfulltest.yml | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 6111d6ab..f711c9ce 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -6,6 +6,8 @@ name: nf-core AWS full size tests
 on:
   release:
     types: [published]
+  pull_request_target:
+    branches: [master]
   workflow_dispatch:
 jobs:
   run-tower:

From a163abb989193c84c12e63ad282fef8210af234f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 19 Apr 2022 16:21:30 +0200
Subject: [PATCH 301/359] revert trigger for aws full test on PR

---
 .github/workflows/awsfulltest.yml | 2 --
 1 file changed, 2 deletions(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index f711c9ce..6111d6ab 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -6,8 +6,6 @@ name: nf-core AWS full size tests
 on:
   release:
     types: [published]
-  pull_request_target:
-    branches: [master]
   workflow_dispatch:
 jobs:
   run-tower:

From 83b37a047bd6fe4519c980843e01274435312a71 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 20 Apr 2022 10:30:54 +0200
Subject: [PATCH 302/359] Update README.md

Co-authored-by: Martin Proks <mproksik@gmail.com>
---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 58faab7d..e86398d3 100644
--- a/README.md
+++ b/README.md
@@ -84,7 +84,7 @@ In rnafusion the full-sized test includes reference building and fusion detectio
 11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 12. QC for mapped reads ([`QualiMap: BAM QC`](https://kokonech.github.io/qualimap/HG00096.chr20_bamqc/qualimapReport.html))
 13. Index mapped reads ([samtools index`](http://www.htslib.org/))
-14. Collect metrics ([picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)) and ([picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))
+14. Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))
 
 ## Quick Start
 

From 8637e6e18a27992fb9c871830aee0c6f9c33383f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 20 Apr 2022 12:01:40 +0200
Subject: [PATCH 303/359] Update README.md

Co-authored-by: Martin Proks <mproksik@gmail.com>
---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index e86398d3..c680581f 100644
--- a/README.md
+++ b/README.md
@@ -1,4 +1,4 @@
-# ![nf-core/rnafusion](docs/images/nf-core/rnafusion_logo_light.png#gh-light-mode-only) ![nf-core/rnafusion](docs/images/nf-core/rnafusion_logo_dark.png#gh-dark-mode-only)
+# ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_light.png#gh-light-mode-only) ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_dark.png#gh-dark-mode-only)
 
 [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+CI%22)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)

From 3d3725b1b379f6733a6c81740d8f288355f0dd22 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 22 Apr 2022 10:45:06 +0200
Subject: [PATCH 304/359] Update README.md

Co-authored-by: Martin Proks <mproksik@gmail.com>
---
 README.md | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/README.md b/README.md
index c680581f..d65c54d6 100644
--- a/README.md
+++ b/README.md
@@ -57,9 +57,9 @@ In rnafusion the full-sized test includes reference building and fusion detectio
 2. Concatenate fastq files per sample
 3. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
 4. Arriba subworkflow
-   - STAR alignment
-   - Samtool sort
-   - Samtool index
+   - [STAR](https://github.com/alexdobin/STAR) alignment
+   - [Samtool](https://github.com/samtools/samtools) sort
+   - [Samtool](https://github.com/samtools/samtools) index
    - [Arriba](https://github.com/suhrig/arriba) fusion detection
    - [Arriba](https://github.com/suhrig/arriba) visualisation
 5. Pizzly subworkflow

From dfafabc13bbfb17ac01958fc7b2501271e60c00f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 22 Apr 2022 10:45:27 +0200
Subject: [PATCH 305/359] Update README.md

Co-authored-by: Martin Proks <mproksik@gmail.com>
---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index d65c54d6..1288e8c8 100644
--- a/README.md
+++ b/README.md
@@ -83,7 +83,7 @@ In rnafusion the full-sized test includes reference building and fusion detectio
     - [FusionInspector](https://github.com/FusionInspector/FusionInspector)
 11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 12. QC for mapped reads ([`QualiMap: BAM QC`](https://kokonech.github.io/qualimap/HG00096.chr20_bamqc/qualimapReport.html))
-13. Index mapped reads ([samtools index`](http://www.htslib.org/))
+13. Index mapped reads ([samtools index](http://www.htslib.org/))
 14. Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))
 
 ## Quick Start

From a4679545fc2f8ecbf5f49abf37394500fe59bcb3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 22 Apr 2022 10:45:36 +0200
Subject: [PATCH 306/359] Update README.md

Co-authored-by: Martin Proks <mproksik@gmail.com>
---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 1288e8c8..618ebd03 100644
--- a/README.md
+++ b/README.md
@@ -119,7 +119,7 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline [usag
 
 ## Credits
 
-nf-core/rnafusion was originally written by Martin Proks (matq007), Praveen Raj (@praveenraj2018).
+nf-core/rnafusion was originally written by Martin Proks ([@matq007](https://github.com/matq007)), Praveen Raj ([@praveenraj2018](https://github.com/praveenraj2018))
 
 We thank the following people for their extensive assistance in the development of this pipeline:
 

From b9c0dafad6f710be176d71558b6ece413f835c73 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 22 Apr 2022 10:48:42 +0200
Subject: [PATCH 307/359] Update formatting as for review comment

---
 README.md | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/README.md b/README.md
index 618ebd03..830453e0 100644
--- a/README.md
+++ b/README.md
@@ -121,9 +121,9 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline [usag
 
 nf-core/rnafusion was originally written by Martin Proks ([@matq007](https://github.com/matq007)), Praveen Raj ([@praveenraj2018](https://github.com/praveenraj2018))
 
-We thank the following people for their extensive assistance in the development of this pipeline:
+We thank the following people for their **extensive assistance** in the development of this pipeline:
 
-- Annick Renevey (@rannick)
+- Annick Renevey ([@rannick](https://github.com/rannick))
 
 ## Contributions and Support
 

From 1c7410f47c6ef4075a6d9bb28b3e4200ad396410 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 22 Apr 2022 13:27:12 +0200
Subject: [PATCH 308/359] set STRAND_SPECIFICITY to NONE for unstranded fastq
 files

---
 modules/local/picard/collectrnaseqmetrics/main.nf | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/modules/local/picard/collectrnaseqmetrics/main.nf b/modules/local/picard/collectrnaseqmetrics/main.nf
index 221f507b..d01c6534 100644
--- a/modules/local/picard/collectrnaseqmetrics/main.nf
+++ b/modules/local/picard/collectrnaseqmetrics/main.nf
@@ -26,7 +26,10 @@ process PICARD_COLLECTRNASEQMETRICS {
         strandedness = '--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND'
     } else if ("${meta.strandedness}" == 'reverse') {
         strandedness = '--STRAND_SPECIFICITY SECOND_READ_TRANSCRIPTION_STRAND'
+    } else {
+        strandedness = '--STRAND_SPECIFICITY NONE'
     }
+
     def rrna = rrna_intervals == [] ? '' : "--RIBOSOMAL_INTERVALS ${rrna_intervals}"
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"

From 1f17d91dba4a9fbd271530ab8b8ac9a085d03275 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 29 Apr 2022 19:04:53 +0200
Subject: [PATCH 309/359] fix refflat linking with starfusion download

---
 workflows/rnafusion.nf | 5 +++--
 1 file changed, 3 insertions(+), 2 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 9368244e..0eff976d 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -16,15 +16,17 @@ if (file(params.input).exists() || params.build_references) { ch_input = file(pa
 
 ch_chrgtf = params.starfusion_build ? file(params.chrgtf) : file("${params.starfusion_ref}/ref_annot.gtf")
 ch_starindex_ref = params.starfusion_build ? params.starindex_ref : "${params.starfusion_ref}/ref_genome.fa.star.idx"
+ch_refflat = params.starfusion_build ? file(params.refflat) : "${params.ensembl_ref}/ref_annot.gtf.refflat"
 
 def checkPathParamList = [
     params.fasta,
     params.gtf,
     ch_chrgtf,
     params.transcript,
-    // params.refflat
+    ch_refflat
 ]
 
+
 for (param in checkPathParamList) if ((param) && !params.build_references) file(param, checkIfExists: true)
 for (param in checkPathParamList) if ((param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }
 if ((params.squid || params.all) && params.ensembl_version == 105) { exit 1, 'Ensembl version 105 is not supported by squid' }
@@ -32,7 +34,6 @@ if ((params.squid || params.all) && params.ensembl_version == 105) { exit 1, 'En
 ch_fasta = file(params.fasta)
 ch_gtf = file(params.gtf)
 ch_transcript = file(params.transcript)
-ch_refflat = file(params.refflat)
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From 1e1c81f55ac9449eabfccc55b98c5419cecc0f38 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 29 Apr 2022 19:13:55 +0200
Subject: [PATCH 310/359] add container for GET_PATH process for AWS batch
 system

---
 modules/local/getpath/main.nf | 4 ++++
 1 file changed, 4 insertions(+)

diff --git a/modules/local/getpath/main.nf b/modules/local/getpath/main.nf
index 7bc72c0d..79ce70d5 100644
--- a/modules/local/getpath/main.nf
+++ b/modules/local/getpath/main.nf
@@ -2,6 +2,10 @@ process GET_PATH {
     tag 'getpath'
     label 'process_low'
 
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
+        'biocontainers/biocontainers:v1.2.0_cv1' }"
+
     input:
     tuple val(meta), path(file)
 

From 1acd5333bd223c648feddf1161ce28ac641112ca Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 2 May 2022 17:35:11 +0200
Subject: [PATCH 311/359] add a get meta and update getpath container

---
 modules/local/getmeta/main.nf | 20 ++++++++++++++++++++
 modules/local/getpath/main.nf |  5 +++++
 2 files changed, 25 insertions(+)
 create mode 100644 modules/local/getmeta/main.nf

diff --git a/modules/local/getmeta/main.nf b/modules/local/getmeta/main.nf
new file mode 100644
index 00000000..c52b16dc
--- /dev/null
+++ b/modules/local/getmeta/main.nf
@@ -0,0 +1,20 @@
+process GET_META {
+    tag 'getmeta'
+    label 'process_low'
+
+    conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
+        'biocontainers/biocontainers:v1.2.0_cv1' }"
+
+    input:
+    tuple val(meta), path(reads)
+    path file
+
+    output:
+    tuple val(meta), path(file)     , emit: file
+
+    script:
+    """
+    """
+}
diff --git a/modules/local/getpath/main.nf b/modules/local/getpath/main.nf
index 7bc72c0d..5f159287 100644
--- a/modules/local/getpath/main.nf
+++ b/modules/local/getpath/main.nf
@@ -2,6 +2,11 @@ process GET_PATH {
     tag 'getpath'
     label 'process_low'
 
+    conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
+        'biocontainers/biocontainers:v1.2.0_cv1' }"
+
     input:
     tuple val(meta), path(file)
 

From b255bbf8cf039448a377d5ac6f2b25d18b9bcfd0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 2 May 2022 17:35:47 +0200
Subject: [PATCH 312/359] workflow output fusion channels with meta information

---
 subworkflows/local/arriba_workflow.nf        |  9 ++++-----
 subworkflows/local/fusioncatcher_workflow.nf | 11 +++++------
 subworkflows/local/pizzly_workflow.nf        | 13 +++++--------
 subworkflows/local/squid_workflow.nf         |  9 ++++-----
 subworkflows/local/starfusion_workflow.nf    | 13 +++++--------
 5 files changed, 23 insertions(+), 32 deletions(-)

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index d468148d..68bcefbe 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -1,6 +1,6 @@
 include { ARRIBA }                                      from '../../modules/nf-core/modules/arriba/main'
 include { ARRIBA_VISUALISATION }                        from '../../modules/local/arriba/visualisation/main'
-include { GET_PATH as GET_PATH_ARRIBA }                 from '../../modules/local/getpath/main'
+include { GET_META }                                    from '../../modules/local/getmeta/main'
 include { GET_PATH as GET_PATH_ARRIBA_FAIL }            from '../../modules/local/getpath/main'
 include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_ARRIBA }   from '../../modules/nf-core/modules/samtools/sort/main'
 include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_ARRIBA}  from '../../modules/nf-core/modules/samtools/index/main'
@@ -32,14 +32,13 @@ workflow ARRIBA_WORKFLOW {
             bam_indexed = SAMTOOLS_SORT_FOR_ARRIBA.out.bam.join(SAMTOOLS_INDEX_FOR_ARRIBA.out.bai)
 
             if (params.arriba_fusions) {
-                ch_arriba_fusions = params.arriba_fusions
+                ch_arriba_fusions = GET_META(reads, params.arriba_fusions)
                 ch_arriba_fusion_fail = ch_dummy_file
             } else {
                 ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf )
                 ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
-                GET_PATH_ARRIBA(ARRIBA.out.fusions)
-                ch_arriba_fusions = GET_PATH_ARRIBA.out.file
+                ch_arriba_fusions = ARRIBA.out.fusions
 
                 GET_PATH_ARRIBA_FAIL(ARRIBA.out.fusions_fail)
                 ch_arriba_fusion_fail = GET_PATH_ARRIBA_FAIL.out.file
@@ -52,7 +51,7 @@ workflow ARRIBA_WORKFLOW {
 
         }
         else {
-            ch_arriba_fusions       = ch_dummy_file
+            ch_arriba_fusions       = GET_META(reads, ch_dummy_file)
             ch_arriba_fusion_fail   = ch_dummy_file
             ch_arriba_visualisation = ch_dummy_file
         }
diff --git a/subworkflows/local/fusioncatcher_workflow.nf b/subworkflows/local/fusioncatcher_workflow.nf
index 53ebdcb6..bbf771ab 100644
--- a/subworkflows/local/fusioncatcher_workflow.nf
+++ b/subworkflows/local/fusioncatcher_workflow.nf
@@ -1,5 +1,5 @@
-include { FUSIONCATCHER }          from '../../modules/local/fusioncatcher/detect/main'
-include { GET_PATH }               from '../../modules/local/getpath/main'
+include { FUSIONCATCHER }                       from '../../modules/local/fusioncatcher/detect/main'
+include { GET_META }                            from '../../modules/local/getmeta/main'
 
 
 workflow FUSIONCATCHER_WORKFLOW {
@@ -12,18 +12,17 @@ workflow FUSIONCATCHER_WORKFLOW {
 
         if (params.fusioncatcher || params.all) {
             if (params.fusioncatcher_fusions){
-                ch_fusioncatcher_fusions = params.fusioncatcher_fusions
+                ch_fusioncatcher_fusions = GET_META(reads, params.fusioncatcher_fusions)
             } else {
                 FUSIONCATCHER (
                     reads,
                     params.fusioncatcher_ref
                 )
-                GET_PATH(FUSIONCATCHER.out.fusions)
-                ch_fusioncatcher_fusions = GET_PATH.out.file
+                ch_fusioncatcher_fusions = FUSIONCATCHER.out.fusions
             }
         }
         else {
-            ch_fusioncatcher_fusions = ch_dummy_file
+            ch_fusioncatcher_fusions = GET_META(reads, ch_dummy_file)
         }
 
     emit:
diff --git a/subworkflows/local/pizzly_workflow.nf b/subworkflows/local/pizzly_workflow.nf
index 7ef281e4..1c73697f 100644
--- a/subworkflows/local/pizzly_workflow.nf
+++ b/subworkflows/local/pizzly_workflow.nf
@@ -1,7 +1,6 @@
 include { KALLISTO_QUANT    }     from '../../modules/local/kallisto/quant/main'
 include { PIZZLY            }     from '../../modules/local/pizzly/detect/main'
-include { GET_PATH          }     from '../../modules/local/getpath/main'
-
+include { GET_META          }     from '../../modules/local/getmeta/main'
 
 workflow PIZZLY_WORKFLOW {
     take:
@@ -15,21 +14,19 @@ workflow PIZZLY_WORKFLOW {
 
         if (params.pizzly || params.all) {
             if (params.pizzly_fusions) {
-                ch_pizzly_fusions = params.pizzly_fusions
+                ch_pizzly_fusions = GET_META(reads, params.pizzly_fusions)
             } else {
-
-                KALLISTO_QUANT( reads, params.pizzly_ref )
+                KALLISTO_QUANT(reads, params.pizzly_ref )
                 ch_versions = ch_versions.mix(KALLISTO_QUANT.out.versions)
 
                 PIZZLY( KALLISTO_QUANT.out.txt, ch_transcript, ch_gtf )
                 ch_versions = ch_versions.mix(PIZZLY.out.versions)
 
-                GET_PATH(PIZZLY.out.fusions)
-                ch_pizzly_fusions = GET_PATH.out.file
+                ch_pizzly_fusions = PIZZLY.out.fusions
             }
         }
         else  {
-            ch_pizzly_fusions = ch_dummy_file
+            ch_pizzly_fusions = GET_META(reads, ch_dummy_file)
 
         }
 
diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 1dda8633..83116ede 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -1,4 +1,4 @@
-include { GET_PATH }                                    from '../../modules/local/getpath/main'
+include { GET_META }                                    from '../../modules/local/getmeta/main'
 include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_SQUID }    from '../../modules/nf-core/modules/samtools/sort/main'
 include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_SQUID }    from '../../modules/nf-core/modules/samtools/view/main'
 include { SQUID }                                       from '../../modules/local/squid/detect/main'
@@ -18,7 +18,7 @@ workflow SQUID_WORKFLOW {
 
         if (params.squid || params.all) {
             if (params.squid_fusions){
-                ch_squid_fusions = params.squid_fusions
+                ch_squid_fusions = GET_META(reads, params.squid_fusions)
             } else {
 
             STAR_FOR_SQUID( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
@@ -38,12 +38,11 @@ workflow SQUID_WORKFLOW {
             SQUID_ANNOTATE ( SQUID.out.fusions, ch_gtf )
             ch_versions = ch_versions.mix(SQUID_ANNOTATE.out.versions)
 
-            GET_PATH(SQUID_ANNOTATE.out.fusions_annotated)
-            ch_squid_fusions = GET_PATH.out.file
+            ch_squid_fusions = SQUID_ANNOTATE.out.fusions_annotated
             }
         }
         else {
-            ch_squid_fusions = ch_dummy_file
+            ch_squid_fusions = GET_META(reads, ch_dummy_file)
         }
 
     emit:
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index d95e5f81..5bf606aa 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -1,7 +1,6 @@
 include { STAR_ALIGN as STAR_FOR_STARFUSION }    from '../../modules/nf-core/modules/star/align/main'
 include { STARFUSION }                           from '../../modules/local/starfusion/detect/main'
-include { GET_PATH }                             from '../../modules/local/getpath/main'
-
+include { GET_META }                             from '../../modules/local/getmeta/main'
 
 workflow STARFUSION_WORKFLOW {
     take:
@@ -16,7 +15,7 @@ workflow STARFUSION_WORKFLOW {
 
         if (params.starfusion || params.all){
             if (params.starfusion_fusions){
-                ch_starfusion_fusions = params.starfusion_fusions
+                ch_starfusion_fusions = GET_META(reads, params.starfusion_fusions)
             } else {
                 STAR_FOR_STARFUSION( reads, ch_starindex_ref, ch_chrgtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
@@ -27,18 +26,16 @@ workflow STARFUSION_WORKFLOW {
                 STARFUSION( reads_junction, params.starfusion_ref)
                 ch_versions = ch_versions.mix(STARFUSION.out.versions)
 
-                GET_PATH(STARFUSION.out.fusions)
-                ch_starfusion_fusions = GET_PATH.out.file
+                ch_starfusion_fusions = STARFUSION.out.fusions
             }
         }
         else {
-            ch_starfusion_fusions = ch_dummy_file
+            ch_starfusion_fusions = GET_META(reads, ch_dummy_file)
         }
     emit:
         fusions         = ch_starfusion_fusions
         bam_sorted      = ch_align
         versions        = ch_versions.ifEmpty(null)
 
-
-}
+    }
 

From abac2613f1bf89006aa29bcba99d65823804a790 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 2 May 2022 17:36:31 +0200
Subject: [PATCH 313/359] adapt processes that  take fusions to include a meta
 tuple

---
 modules/local/arriba/visualisation/main.nf |  2 +-
 modules/local/fusionreport/detect/main.nf  | 12 ++++++------
 2 files changed, 7 insertions(+), 7 deletions(-)

diff --git a/modules/local/arriba/visualisation/main.nf b/modules/local/arriba/visualisation/main.nf
index 7dbbe23b..52caf995 100644
--- a/modules/local/arriba/visualisation/main.nf
+++ b/modules/local/arriba/visualisation/main.nf
@@ -9,7 +9,7 @@ process ARRIBA_VISUALISATION {
 
     input:
     tuple val(meta), path(bam), path(bai)
-    path fusions
+    tuple val(meta), path(fusions)
     path reference
     path gtf
 
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index badf5937..b52d37e2 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -11,12 +11,12 @@ process FUSIONREPORT {
 
     input:
     tuple val(meta), path(reads)
-    path(fusionreport_ref)
-    path(arriba_fusions)
-    path(pizzly_fusions)
-    path(squid_fusions)
-    path(starfusion_fusions)
-    path(fusioncatcher_fusions)
+    tuple val(meta), path(fusionreport_ref)
+    tuple val(meta), path(arriba_fusions)
+    tuple val(meta), path(pizzly_fusions)
+    tuple val(meta), path(squid_fusions)
+    tuple val(meta), path(starfusion_fusions)
+    tuple val(meta), path(fusioncatcher_fusions)
 
     output:
     path "versions.yml"                                  , emit: versions

From 09da1115a61aeed5d9a97e689b59ac22f698d0e0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 2 May 2022 17:37:02 +0200
Subject: [PATCH 314/359] exclude s3 paths from absolute path check

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 9368244e..c7ec0ca9 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -26,7 +26,7 @@ def checkPathParamList = [
 ]
 
 for (param in checkPathParamList) if ((param) && !params.build_references) file(param, checkIfExists: true)
-for (param in checkPathParamList) if ((param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }
+for (param in checkPathParamList) if ((param.toString())!= file(param).toString() && !params.build_references && !("s3" in param.toString())) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }
 if ((params.squid || params.all) && params.ensembl_version == 105) { exit 1, 'Ensembl version 105 is not supported by squid' }
 
 ch_fasta = file(params.fasta)

From 95e91512077a43b46853dc23ded353c7738f5d3d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 3 May 2022 17:05:15 +0200
Subject: [PATCH 315/359] remove typo

---
 modules/local/fusioncatcher/detect/main.nf | 1 -
 1 file changed, 1 deletion(-)

diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 480b9f9d..08c3e788 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -43,6 +43,5 @@ process FUSIONCATCHER {
     "${task.process}":
         fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1)| sed 's/fusioncatcher.py //')
     END_VERSIONS
-    touch versions.yml
     """
 }

From a469cbb23b7118e7bdbbeff88756ef1bc457b8e4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 3 May 2022 17:05:41 +0200
Subject: [PATCH 316/359] s3 check before evaluating param.toString()

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 90199b68..a131111c 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -28,7 +28,7 @@ def checkPathParamList = [
 
 
 for (param in checkPathParamList) if ((param) && !params.build_references) file(param, checkIfExists: true)
-for (param in checkPathParamList) if ((param.toString())!= file(param).toString() && !params.build_references && !("s3" in param.toString())) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }
+for (param in checkPathParamList) if (!("s3" in param) && (param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }
 if ((params.squid || params.all) && params.ensembl_version == 105) { exit 1, 'Ensembl version 105 is not supported by squid' }
 
 ch_fasta = file(params.fasta)

From 805dfe8554e8f7913fd66280acedec2aab7a9df9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 3 May 2022 17:40:38 +0200
Subject: [PATCH 317/359] fix of s3 paths?

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index a131111c..69c5db5a 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -28,7 +28,7 @@ def checkPathParamList = [
 
 
 for (param in checkPathParamList) if ((param) && !params.build_references) file(param, checkIfExists: true)
-for (param in checkPathParamList) if (!("s3" in param) && (param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }
+for (param in checkPathParamList) if (!("s3" in param)) {if ((param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }}
 if ((params.squid || params.all) && params.ensembl_version == 105) { exit 1, 'Ensembl version 105 is not supported by squid' }
 
 ch_fasta = file(params.fasta)

From 109a0159b05eca7fcd7778a7dc2e25439e2bdb35 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 3 May 2022 18:08:11 +0200
Subject: [PATCH 318/359] squid uses ensembl 102 refs

---
 subworkflows/local/squid_workflow.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 83116ede..75f24775 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -10,7 +10,7 @@ workflow SQUID_WORKFLOW {
     take:
         reads
         ch_gtf
-        ch_starindex_ref
+        ch_starindex_ensembl_ref
 
     main:
         ch_versions = Channel.empty()
@@ -21,7 +21,7 @@ workflow SQUID_WORKFLOW {
                 ch_squid_fusions = GET_META(reads, params.squid_fusions)
             } else {
 
-            STAR_FOR_SQUID( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
+            STAR_FOR_SQUID( reads, ch_starindex_ensembl_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
             SAMTOOLS_VIEW_FOR_SQUID ( STAR_FOR_SQUID.out.sam, [] )

From e2b2dfb686b750ec63b3970a512ce0730dfbcfe1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 3 May 2022 18:12:25 +0200
Subject: [PATCH 319/359] squid uses ensembl 102 refs

---
 workflows/rnafusion.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 69c5db5a..4bf116cb 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -16,6 +16,7 @@ if (file(params.input).exists() || params.build_references) { ch_input = file(pa
 
 ch_chrgtf = params.starfusion_build ? file(params.chrgtf) : file("${params.starfusion_ref}/ref_annot.gtf")
 ch_starindex_ref = params.starfusion_build ? params.starindex_ref : "${params.starfusion_ref}/ref_genome.fa.star.idx"
+ch_starindex_ensembl_ref = params.starindex_ref
 ch_refflat = params.starfusion_build ? file(params.refflat) : "${params.ensembl_ref}/ref_annot.gtf.refflat"
 
 def checkPathParamList = [
@@ -160,7 +161,7 @@ workflow RNAFUSION {
     SQUID_WORKFLOW (
         ch_cat_fastq,
         ch_gtf,
-        ch_starindex_ref
+        ch_starindex_ensembl_ref
     )
     ch_versions = ch_versions.mix(SQUID_WORKFLOW.out.versions.first().ifEmpty(null))
 

From 83cb60b59daa4639bdd6cbe4003a80c390b06e06 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 3 May 2022 18:20:36 +0200
Subject: [PATCH 320/359] fix of s3 paths? v2

---
 workflows/rnafusion.nf | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 69c5db5a..46f32c37 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -28,7 +28,9 @@ def checkPathParamList = [
 
 
 for (param in checkPathParamList) if ((param) && !params.build_references) file(param, checkIfExists: true)
-for (param in checkPathParamList) if (!("s3" in param)) {if ((param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }}
+if (!("s3" in params.fasta)) {
+    for (param in checkPathParamList) if ((param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }
+}
 if ((params.squid || params.all) && params.ensembl_version == 105) { exit 1, 'Ensembl version 105 is not supported by squid' }
 
 ch_fasta = file(params.fasta)

From 9ec72e4686ed079ad77c1d78747bd2db922e48d5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 3 May 2022 18:53:13 +0200
Subject: [PATCH 321/359] fix of s3 paths? v3

---
 workflows/rnafusion.nf | 5 ++++-
 1 file changed, 4 insertions(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 46f32c37..13ada600 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -28,7 +28,10 @@ def checkPathParamList = [
 
 
 for (param in checkPathParamList) if ((param) && !params.build_references) file(param, checkIfExists: true)
-if (!("s3" in params.fasta)) {
+if (params.fasta[0,1] == "s3") {
+    log.info "INFO: s3 path detected, check for absolute path and trailing '/' not performed"
+}
+else {
     for (param in checkPathParamList) if ((param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." }
 }
 if ((params.squid || params.all) && params.ensembl_version == 105) { exit 1, 'Ensembl version 105 is not supported by squid' }

From 6e0b677281bbe1dcad9e9afb541bb327efa8ec7c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 May 2022 13:43:10 +0200
Subject: [PATCH 322/359] fusionreport_ref does not have meta info

---
 modules/local/fusionreport/detect/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index b52d37e2..f51f6758 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -11,7 +11,7 @@ process FUSIONREPORT {
 
     input:
     tuple val(meta), path(reads)
-    tuple val(meta), path(fusionreport_ref)
+    path(fusionreport_ref)
     tuple val(meta), path(arriba_fusions)
     tuple val(meta), path(pizzly_fusions)
     tuple val(meta), path(squid_fusions)

From 1e96beda1b48318ff84b0ae2edcb6f71331f7fc9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 May 2022 16:58:15 +0200
Subject: [PATCH 323/359] remove collected_fusion folder

---
 conf/modules.config | 8 --------
 1 file changed, 8 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index e9736b92..56597f80 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -102,14 +102,6 @@ process {
         ]
     }
 
-    withName: GET_PATH {
-        publishDir = [
-            path: { "${params.outdir}/collected_fusions" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
-        ]
-    }
-
     withName: GTF_TO_REFFLAT {
         publishDir = [
             path: { "${params.genomes_base}/ensembl" },

From 68c0892dd173d3e22b1af347b8f4f5cf4c350b48 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 May 2022 16:58:46 +0200
Subject: [PATCH 324/359] join channels from reads and fusions to send fusions
 per sample

---
 modules/local/fusionreport/detect/main.nf   | 7 +------
 subworkflows/local/fusionreport_workflow.nf | 9 ++++++++-
 2 files changed, 9 insertions(+), 7 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index f51f6758..ddf4667b 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -10,13 +10,8 @@ process FUSIONREPORT {
 
 
     input:
-    tuple val(meta), path(reads)
+    tuple val(meta), path(reads), path(arriba_fusions), path(pizzly_fusions), path(squid_fusions), path(starfusion_fusions),  path(fusioncatcher_fusions)
     path(fusionreport_ref)
-    tuple val(meta), path(arriba_fusions)
-    tuple val(meta), path(pizzly_fusions)
-    tuple val(meta), path(squid_fusions)
-    tuple val(meta), path(starfusion_fusions)
-    tuple val(meta), path(fusioncatcher_fusions)
 
     output:
     path "versions.yml"                                  , emit: versions
diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index 085b08f3..b0de9f4a 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -14,7 +14,14 @@ workflow FUSIONREPORT_WORKFLOW {
     main:
         ch_versions = Channel.empty()
 
-        FUSIONREPORT( reads, fusionreport_ref, arriba_fusions, pizzly_fusions, squid_fusions, starfusion_fusions, fusioncatcher_fusions )
+        reads_fusions = reads
+        .join(arriba_fusions, remainder: true)
+        .join(pizzly_fusions, remainder: true)
+        .join(squid_fusions, remainder: true)
+        .join(starfusion_fusions, remainder: true)
+        .join(fusioncatcher_fusions, remainder: true)
+
+        FUSIONREPORT( reads_fusions, fusionreport_ref)
         ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
 
     emit:

From 2c3d0958d70fef914b09840988ffdfa6dbe928f5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 6 May 2022 13:31:49 +0200
Subject: [PATCH 325/359] add blacklist and protein domain via input instead of
 args by changing arriba module (tmp fix)

---
 conf/modules.config                    |  4 ++--
 modules/nf-core/modules/arriba/main.nf | 18 +++++++++++++++++-
 nextflow.config                        |  2 ++
 nextflow_schema.json                   | 10 ++++++++++
 subworkflows/local/arriba_workflow.nf  |  2 +-
 5 files changed, 32 insertions(+), 4 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 56597f80..ebd599e3 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -24,8 +24,8 @@ process {
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
-        ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv.gz \
-        -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
+        // ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv.gz \
+        // -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
         ext.prefix =  { "${meta.id}.arriba" }
     }
 
diff --git a/modules/nf-core/modules/arriba/main.nf b/modules/nf-core/modules/arriba/main.nf
index e0d30b45..b2081707 100644
--- a/modules/nf-core/modules/arriba/main.nf
+++ b/modules/nf-core/modules/arriba/main.nf
@@ -11,6 +11,12 @@ process ARRIBA {
     tuple val(meta), path(bam)
     path fasta
     path gtf
+    path blacklist
+    path known_fusions
+    path structural_variants
+    path tags
+    path protein_domains
+
 
     output:
     tuple val(meta), path("*.fusions.tsv")          , emit: fusions
@@ -23,7 +29,13 @@ process ARRIBA {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def blacklist = (args.contains('-b')) ? '' : '-f blacklist'
+    def blacklist = blacklist ? "-b $blacklist" : ""
+    def known_fusions = known_fusions ? "-k $known_fusions" : ""
+    def structural_variants = structural_variants ? "-d $structual_variants" : ""
+    def tags = tags ? "-t $tags" : ""
+    def protein_domains = protein_domains ? "-p $protein_domains" : ""
+
+
     """
     arriba \\
         -x $bam \\
@@ -32,6 +44,10 @@ process ARRIBA {
         -o ${prefix}.fusions.tsv \\
         -O ${prefix}.fusions.discarded.tsv \\
         $blacklist \\
+        $known_fusions \\
+        $structural_variants \\
+        $tags \\
+        $protein_domains \\
         $args
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/nextflow.config b/nextflow.config
index 061d63b3..c14df082 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -52,6 +52,8 @@ params {
     // Path to references
     ensembl_ref                   = "${params.genomes_base}/ensembl"
     arriba_ref                    = "${params.genomes_base}/arriba"
+    arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.1.0.tsv.gz"
+    arriba_ref_protein_domain     = "${params.genomes_base}/arriba/protein_domains_hg38_GRCh38_v2.1.0.gff3"
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v102"
     pizzly_ref                    = "${params.genomes_base}/pizzly/kallisto"
     squid_ref                     = "${params.genomes_base}/squid"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 674c124a..8d68e568 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -99,6 +99,16 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba references"
                 },
+                "arriba_ref_blacklist": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to arriba reference blacklist"
+                },
+                "arriba_ref_protein_domain": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to arriba reference protein domain"
+                },
                 "arriba_fusions": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 68bcefbe..981c9b33 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -35,7 +35,7 @@ workflow ARRIBA_WORKFLOW {
                 ch_arriba_fusions = GET_META(reads, params.arriba_fusions)
                 ch_arriba_fusion_fail = ch_dummy_file
             } else {
-                ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf )
+                ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf, params.arriba_ref_blacklist, [], [], [], params.arriba_ref_protein_domain )
                 ch_versions = ch_versions.mix(ARRIBA.out.versions)
 
                 ch_arriba_fusions = ARRIBA.out.fusions

From bb41caddd271f2691814d7846d88790eb9100ed6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 May 2022 13:14:02 +0200
Subject: [PATCH 326/359] update arriba module from nf-core

---
 modules/nf-core/modules/arriba/main.nf  | 20 ++++++++++++++------
 modules/nf-core/modules/arriba/meta.yml | 22 +++++++++++++++++++++-
 2 files changed, 35 insertions(+), 7 deletions(-)

diff --git a/modules/nf-core/modules/arriba/main.nf b/modules/nf-core/modules/arriba/main.nf
index b2081707..b7883acd 100644
--- a/modules/nf-core/modules/arriba/main.nf
+++ b/modules/nf-core/modules/arriba/main.nf
@@ -2,10 +2,10 @@ process ARRIBA {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::arriba=2.1.0" : null)
+    conda (params.enable_conda ? "bioconda::arriba=2.2.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/arriba:2.1.0--h3198e80_1' :
-        'quay.io/biocontainers/arriba:2.1.0--h3198e80_1' }"
+        'https://depot.galaxyproject.org/singularity/arriba:2.2.1--hecb563c_2' :
+        'quay.io/biocontainers/arriba:2.2.1--hecb563c_2' }"
 
     input:
     tuple val(meta), path(bam)
@@ -17,7 +17,6 @@ process ARRIBA {
     path tags
     path protein_domains
 
-
     output:
     tuple val(meta), path("*.fusions.tsv")          , emit: fusions
     tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail
@@ -29,13 +28,12 @@ process ARRIBA {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def blacklist = blacklist ? "-b $blacklist" : ""
+    def blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
     def known_fusions = known_fusions ? "-k $known_fusions" : ""
     def structural_variants = structural_variants ? "-d $structual_variants" : ""
     def tags = tags ? "-t $tags" : ""
     def protein_domains = protein_domains ? "-p $protein_domains" : ""
 
-
     """
     arriba \\
         -x $bam \\
@@ -55,4 +53,14 @@ process ARRIBA {
         arriba: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    echo stub > ${prefix}.fusions.tsv
+    echo stub > ${prefix}.fusions.discarded.tsv
+
+    echo "${task.process}:" > versions.yml
+    echo ' arriba: 2.2.1' >> versions.yml
+    """
 }
diff --git a/modules/nf-core/modules/arriba/meta.yml b/modules/nf-core/modules/arriba/meta.yml
index 4bde2f08..119dd912 100644
--- a/modules/nf-core/modules/arriba/meta.yml
+++ b/modules/nf-core/modules/arriba/meta.yml
@@ -30,6 +30,26 @@ input:
       type: file
       description: Annotation GTF file
       pattern: "*.{gtf}"
+  - blacklist:
+      type: file
+      description: Blacklist file
+      pattern: "*.{tsv}"
+  - known_fusions:
+      type: file
+      description: Known fusions file
+      pattern: "*.{tsv}"
+  - structural_variants:
+      type: file
+      description: Structural variants file
+      pattern: "*.{tsv}"
+  - tags:
+      type: file
+      description: Tags file
+      pattern: "*.{tsv}"
+  - protein_domains:
+      type: file
+      description: Protein domains file
+      pattern: "*.{gff3}"
 
 output:
   - meta:
@@ -51,4 +71,4 @@ output:
       pattern: "*.{fusions.discarded.tsv}"
 
 authors:
-  - "@praveenraj2018"
+  - "@praveenraj2018,@rannick"

From 341e50e0baa115cf1511b2fe667e40c207ebe23d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 May 2022 13:14:19 +0200
Subject: [PATCH 327/359] update cat/fastq module from nf-core

---
 modules/nf-core/modules/cat/fastq/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/nf-core/modules/cat/fastq/main.nf b/modules/nf-core/modules/cat/fastq/main.nf
index bf0877c3..b6854895 100644
--- a/modules/nf-core/modules/cat/fastq/main.nf
+++ b/modules/nf-core/modules/cat/fastq/main.nf
@@ -4,8 +4,8 @@ process CAT_FASTQ {
 
     conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
-        'biocontainers/biocontainers:v1.2.0_cv1' }"
+        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
+        'ubuntu:20.04' }"
 
     input:
     tuple val(meta), path(reads, stageAs: "input*/*")

From 3f2b82b6ba012a47b83b3a61bd35cbdbfa5ca5ec Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 May 2022 13:14:55 +0200
Subject: [PATCH 328/359] update picard and samtools

---
 .../modules/picard/collectwgsmetrics/main.nf      | 15 ++++++++-------
 .../nf-core/modules/picard/markduplicates/main.nf | 12 ++++++------
 modules/nf-core/modules/samtools/view/main.nf     | 14 +++++++++++++-
 modules/nf-core/modules/samtools/view/meta.yml    |  4 ++++
 4 files changed, 31 insertions(+), 14 deletions(-)

diff --git a/modules/nf-core/modules/picard/collectwgsmetrics/main.nf b/modules/nf-core/modules/picard/collectwgsmetrics/main.nf
index f4efaa4c..e6dd49e9 100644
--- a/modules/nf-core/modules/picard/collectwgsmetrics/main.nf
+++ b/modules/nf-core/modules/picard/collectwgsmetrics/main.nf
@@ -2,13 +2,13 @@ process PICARD_COLLECTWGSMETRICS {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
+    conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
-        'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
+        'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
 
     input:
-    tuple val(meta), path(bam), path(bai)
+    tuple val(meta), path(bam)
     path  fasta
 
     output:
@@ -32,9 +32,10 @@ process PICARD_COLLECTWGSMETRICS {
         -Xmx${avail_mem}g \\
         CollectWgsMetrics \\
         $args \\
-        INPUT=$bam \\
-        OUTPUT=${prefix}.CollectWgsMetrics.coverage_metrics \\
-        REFERENCE_SEQUENCE=$fasta
+        --INPUT $bam \\
+        --OUTPUT ${prefix}.CollectWgsMetrics.coverage_metrics \\
+        --REFERENCE_SEQUENCE $fasta
+
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf
index e754a587..1565c647 100644
--- a/modules/nf-core/modules/picard/markduplicates/main.nf
+++ b/modules/nf-core/modules/picard/markduplicates/main.nf
@@ -2,10 +2,10 @@ process PICARD_MARKDUPLICATES {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
+    conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
-        'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
+        'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam)
@@ -33,9 +33,9 @@ process PICARD_MARKDUPLICATES {
         -Xmx${avail_mem}g \\
         MarkDuplicates \\
         $args \\
-        I=$bam \\
-        O=${prefix}.bam \\
-        M=${prefix}.MarkDuplicates.metrics.txt
+        --INPUT $bam \\
+        --OUTPUT ${prefix}.bam \\
+        --METRICS_FILE ${prefix}.MarkDuplicates.metrics.txt
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/modules/samtools/view/main.nf b/modules/nf-core/modules/samtools/view/main.nf
index 5f14fbbf..55194e88 100644
--- a/modules/nf-core/modules/samtools/view/main.nf
+++ b/modules/nf-core/modules/samtools/view/main.nf
@@ -8,7 +8,7 @@ process SAMTOOLS_VIEW {
         'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
 
     input:
-    tuple val(meta), path(input)
+    tuple val(meta), path(input), path(index)
     path fasta
 
     output:
@@ -41,4 +41,16 @@ process SAMTOOLS_VIEW {
         samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.bam
+    touch ${prefix}.cram
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/modules/samtools/view/meta.yml b/modules/nf-core/modules/samtools/view/meta.yml
index 5604bfa7..a8b43ecc 100644
--- a/modules/nf-core/modules/samtools/view/meta.yml
+++ b/modules/nf-core/modules/samtools/view/meta.yml
@@ -25,6 +25,10 @@ input:
       type: file
       description: BAM/CRAM/SAM file
       pattern: "*.{bam,cram,sam}"
+  - index:
+      type: optional file
+      description: BAM.BAI/CRAM.CRAI file
+      pattern: "*.{.bai,.crai}"
   - fasta:
       type: optional file
       description: Reference file the CRAM was created with

From 61d4b07cbbb14fd3c8559c50cac7572442055328 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 May 2022 13:15:21 +0200
Subject: [PATCH 329/359] update modules.json

---
 modules.json | 12 ++++++------
 1 file changed, 6 insertions(+), 6 deletions(-)

diff --git a/modules.json b/modules.json
index 86bd0f82..b1159859 100644
--- a/modules.json
+++ b/modules.json
@@ -4,10 +4,10 @@
     "repos": {
         "nf-core/modules": {
             "arriba": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+                "git_sha": "bf91f9dd9b62c98175d962bd51dbe2d0b7f91d4c"
             },
             "cat/fastq": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+                "git_sha": "9aadd9a6d3f5964476582319b3a1c54a3e3fe7c9"
             },
             "custom/dumpsoftwareversions": {
                 "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
@@ -22,10 +22,10 @@
                 "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
             },
             "picard/collectwgsmetrics": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
+                "git_sha": "e263f267a7b3c2cbf95372c832e38ba8b76dfd2e"
             },
             "picard/markduplicates": {
-                "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
+                "git_sha": "20ebb89ff97a2665106be9cace5ccb9aa4eed1be"
             },
             "qualimap/rnaseq": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
@@ -43,7 +43,7 @@
                 "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773"
             },
             "samtools/view": {
-                "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773"
+                "git_sha": "6b64f9cb6c3dd3577931cc3cd032d6fb730000ce"
             },
             "star/align": {
                 "git_sha": "1dddf1ce9443e3d93853d86e7a7aab52e5b4d614"
@@ -53,4 +53,4 @@
             }
         }
     }
-}
+}
\ No newline at end of file

From ef4056f7f1c0bf6dae16ef464079d5ed81c28d6b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 May 2022 13:15:54 +0200
Subject: [PATCH 330/359] starfusion_build is enabled by default

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index c14df082..1243d76a 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -26,7 +26,7 @@ params {
     ensembl_version            = 102
     read_length                = 100
     genomes                    = [:]
-    starfusion_build           = false
+    starfusion_build           = true
 
 
 

From 45fbf3f3adf6f664b4188923f7bd42cf1de5cb74 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 May 2022 13:20:10 +0200
Subject: [PATCH 331/359] prettier formatting

---
 modules.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules.json b/modules.json
index b1159859..d9d98cb1 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}

From d7aa74b818d0d6ff896d2295b88d411630d62be6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 May 2022 18:19:12 +0200
Subject: [PATCH 332/359] remove commented arriba args and add samtools_index
 squid publish dir

---
 conf/modules.config | 10 ++++++++--
 1 file changed, 8 insertions(+), 2 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index ebd599e3..a5bbc2cb 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -24,8 +24,6 @@ process {
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
-        // ext.args = "-b ${params.arriba_ref}/blacklist_hg38_GRCh38_v2.1.0.tsv.gz \
-        // -p ${params.arriba_ref}/protein_domains_hg38_GRCh38_v2.1.0.gff3"
         ext.prefix =  { "${meta.id}.arriba" }
     }
 
@@ -166,6 +164,14 @@ process {
         ]
     }
 
+    withName: SAMTOOLS_INDEX_FOR_SQUID {
+        publishDir = [
+            path: { "${params.outdir}/samtools_index_for_squid" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
     withName: SAMTOOLS_SORT_FOR_ARRIBA {
         ext.prefix = { "${meta.id}_sorted" }
         publishDir = [

From 003d90f55cdceb308e67d869887dfdbf403c5bc9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 May 2022 18:19:33 +0200
Subject: [PATCH 333/359] add indexing in squid workflow

---
 subworkflows/local/squid_workflow.nf | 7 ++++++-
 1 file changed, 6 insertions(+), 1 deletion(-)

diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 83116ede..6fa0effc 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -1,4 +1,5 @@
 include { GET_META }                                    from '../../modules/local/getmeta/main'
+include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_SQUID}   from '../../modules/nf-core/modules/samtools/index/main'
 include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_SQUID }    from '../../modules/nf-core/modules/samtools/sort/main'
 include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_SQUID }    from '../../modules/nf-core/modules/samtools/view/main'
 include { SQUID }                                       from '../../modules/local/squid/detect/main'
@@ -24,7 +25,11 @@ workflow SQUID_WORKFLOW {
             STAR_FOR_SQUID( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
-            SAMTOOLS_VIEW_FOR_SQUID ( STAR_FOR_SQUID.out.sam, [] )
+            SAMTOOLS_INDEX_FOR_SQUID(STAR_FOR_SQUID.out.sam)
+
+            sam_indexed = STAR_FOR_SQUID.out.sam.join(SAMTOOLS_INDEX_FOR_SQUID.out.bai)
+
+            SAMTOOLS_VIEW_FOR_SQUID ( sam_indexed, [] )
             ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_SQUID.out.versions )
 
             SAMTOOLS_SORT_FOR_SQUID ( SAMTOOLS_VIEW_FOR_SQUID.out.bam )

From 3bf015705d31d2dffdaf690bbef2085f71735d10 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 May 2022 18:36:10 +0200
Subject: [PATCH 334/359] test samtools view with empty index

---
 subworkflows/local/squid_workflow.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 6fa0effc..d8f214a6 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -25,9 +25,9 @@ workflow SQUID_WORKFLOW {
             STAR_FOR_SQUID( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
-            SAMTOOLS_INDEX_FOR_SQUID(STAR_FOR_SQUID.out.sam)
+            // SAMTOOLS_INDEX_FOR_SQUID(STAR_FOR_SQUID.out.sam)
 
-            sam_indexed = STAR_FOR_SQUID.out.sam.join(SAMTOOLS_INDEX_FOR_SQUID.out.bai)
+            sam_indexed = STAR_FOR_SQUID.out.sam.join([])
 
             SAMTOOLS_VIEW_FOR_SQUID ( sam_indexed, [] )
             ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_SQUID.out.versions )

From 40c10d6385b6112fe828ac5acfac3ee5492f164f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 May 2022 10:30:25 +0200
Subject: [PATCH 335/359] fix channel issue with samtools view

---
 subworkflows/local/squid_workflow.nf | 7 ++++---
 1 file changed, 4 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/squid_workflow.nf b/subworkflows/local/squid_workflow.nf
index 21b68244..5ed00d8a 100644
--- a/subworkflows/local/squid_workflow.nf
+++ b/subworkflows/local/squid_workflow.nf
@@ -25,9 +25,10 @@ workflow SQUID_WORKFLOW {
             STAR_FOR_SQUID( reads, ch_starindex_ensembl_ref, ch_gtf, params.star_ignore_sjdbgtf, params.seq_platform, params.seq_center )
             ch_versions = ch_versions.mix(STAR_FOR_SQUID.out.versions )
 
-            // SAMTOOLS_INDEX_FOR_SQUID(STAR_FOR_SQUID.out.sam)
-
-            sam_indexed = STAR_FOR_SQUID.out.sam.join([])
+            STAR_FOR_SQUID.out.sam
+            .map { meta, sam ->
+            return [meta, sam, []]
+            }.set { sam_indexed }
 
             SAMTOOLS_VIEW_FOR_SQUID ( sam_indexed, [] )
             ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_SQUID.out.versions )

From 45219e0efa452dcf8de042e29e705727f4cf3adb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 May 2022 10:43:09 +0200
Subject: [PATCH 336/359] remove salmon index module

---
 modules.json                                  |  2 +-
 modules/nf-core/modules/salmon/index/main.nf  | 46 -------------------
 modules/nf-core/modules/salmon/index/meta.yml | 36 ---------------
 3 files changed, 1 insertion(+), 83 deletions(-)
 delete mode 100644 modules/nf-core/modules/salmon/index/main.nf
 delete mode 100644 modules/nf-core/modules/salmon/index/meta.yml

diff --git a/modules.json b/modules.json
index d9d98cb1..b1159859 100644
--- a/modules.json
+++ b/modules.json
@@ -53,4 +53,4 @@
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/modules/salmon/index/main.nf b/modules/nf-core/modules/salmon/index/main.nf
deleted file mode 100644
index 737087f9..00000000
--- a/modules/nf-core/modules/salmon/index/main.nf
+++ /dev/null
@@ -1,46 +0,0 @@
-process SALMON_INDEX {
-    tag "$transcript_fasta"
-    label "process_medium"
-
-    conda (params.enable_conda ? 'bioconda::salmon=1.5.2' : null)
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/salmon:1.5.2--h84f40af_0' :
-        'quay.io/biocontainers/salmon:1.5.2--h84f40af_0' }"
-
-    input:
-    path genome_fasta
-    path transcript_fasta
-
-    output:
-    path "salmon"       , emit: index
-    path "versions.yml" , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def get_decoy_ids = "grep '^>' $genome_fasta | cut -d ' ' -f 1 > decoys.txt"
-    def gentrome      = "gentrome.fa"
-    if (genome_fasta.endsWith('.gz')) {
-        get_decoy_ids = "grep '^>' <(gunzip -c $genome_fasta) | cut -d ' ' -f 1 > decoys.txt"
-        gentrome      = "gentrome.fa.gz"
-    }
-    """
-    $get_decoy_ids
-    sed -i.bak -e 's/>//g' decoys.txt
-    cat $transcript_fasta $genome_fasta > $gentrome
-
-    salmon \\
-        index \\
-        --threads $task.cpus \\
-        -t $gentrome \\
-        -d decoys.txt \\
-        $args \\
-        -i salmon
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        salmon: \$(echo \$(salmon --version) | sed -e "s/salmon //g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/modules/salmon/index/meta.yml b/modules/nf-core/modules/salmon/index/meta.yml
deleted file mode 100644
index 53c64152..00000000
--- a/modules/nf-core/modules/salmon/index/meta.yml
+++ /dev/null
@@ -1,36 +0,0 @@
-name: salmon_index
-description: Create index for salmon
-keywords:
-  - index
-  - fasta
-  - genome
-  - reference
-tools:
-  - salmon:
-      description: |
-        Salmon is a tool for wicked-fast transcript quantification from RNA-seq data
-      homepage: https://salmon.readthedocs.io/en/latest/salmon.html
-      manual: https://salmon.readthedocs.io/en/latest/salmon.html
-      doi: 10.1038/nmeth.4197
-      licence: ["GPL-3.0-or-later"]
-input:
-  - genome_fasta:
-      type: file
-      description: Fasta file of the reference genome
-  - transcriptome_fasta:
-      type: file
-      description: Fasta file of the reference transcriptome
-
-output:
-  - index:
-      type: directory
-      description: Folder containing the star index files
-      pattern: "salmon"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
-authors:
-  - "@kevinmenden"
-  - "@drpatelh"

From 694e034627fea315953a2af65f3fdba75b4ec7e0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 May 2022 10:44:17 +0200
Subject: [PATCH 337/359] update software versions in documentation

---
 CHANGELOG.md | 6 +++---
 README.md    | 2 +-
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9c6fbda0..80df0fac 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -19,9 +19,9 @@ Update to DSL2 and newer software/reference versions
 
 ### Changed
 
-- Upgrade default ensembl version to `105`
-- Upgrade to `nf-core/tools v2.3`
-- Upgrade `Arriba v1.2.0` to `Arriba v2.1.0`
+- Upgrade default ensembl version to `102`
+- Upgrade to `nf-core/tools v2.3.2`
+- Upgrade `Arriba v1.2.0` to `Arriba v2.2.1`
 - Upgrade `FusionCatcher v1.20` to `FusionCatcher v1.33`
 - Upgrade `STAR-fusion v1.8.1` to `STAR-fusion v1.10.1`
 - Upgrade `STAR v2.7.1` to `STAR v2.7.9`
diff --git a/README.md b/README.md
index 830453e0..513a9b0b 100644
--- a/README.md
+++ b/README.md
@@ -26,7 +26,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
 
 | Tool                                                      |  Single-end reads  | Version  |
 | --------------------------------------------------------- | :----------------: | :------: |
-| [Arriba](https://github.com/suhrig/arriba)                |        :x:         | `2.1.0`  |
+| [Arriba](https://github.com/suhrig/arriba)                |        :x:         | `2.2.1`  |
 | [FusionCatcher](https://github.com/ndaniel/fusioncatcher) | :white_check_mark: |  `1.33`  |
 | [Fusion-report](https://github.com/matq007/fusion-report) |         -          | `2.1.5`  |
 | [Pizzly](https://github.com/pmelsted/pizzly)              |        :x:         | `0.37.3` |

From 258b1f51727782d31e694063a4f39c0d60ac750d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 May 2022 10:48:39 +0200
Subject: [PATCH 338/359] update documentation to reflect that starfusion_build
 is true by default now

---
 CHANGELOG.md  | 2 +-
 docs/usage.md | 4 ++--
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 80df0fac..8399e7a8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -15,7 +15,7 @@ Update to DSL2 and newer software/reference versions
 - Added `picard MarkDuplicates v2.26.10` from nf-core modules
 - Added `cat/fastqc` from nf-core modules
 - Added possibility for manually feeding the results of fusions from different tools to speed-up reruns
-- STAR-Fusion references can be downloaded or built (--starfusion_build parameter)
+- STAR-Fusion references can be downloaded or built but downloaded references are NOT RECOMMENDED as not thoroughly tested (--starfusion_build parameter is true by default, use --starfusion_build false to use downloaded STAR-Fusion references).
 
 ### Changed
 
diff --git a/docs/usage.md b/docs/usage.md
index 6d8d23d1..413ea667 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -43,12 +43,12 @@ nextflow run nf-core/rnafusion \
 
 This PATH will be the place the references will be saved.
 
-Optional: by default STAR-Fusion references are downloaded. You can also build them from ensembl fasta and gtf files. This allows more flexibility for different organisms but be aware that **this is slow and computationally intensive**:
+Optional: by default STAR-Fusion references are built. You can also download them from CTAT. This allows more flexibility for different organisms but be aware that **this is not fully tested -> not recommended**:
 
 ```bash
 nextflow run nf-core/rnafusion \
 --build_references --starfusion \
---starfusion_build \
+--starfusion_build false \
 --genomes_base <PATH>
 ```
 

From 6e6bf987c6e46a254dba2c7b29022b5c15e3c6fa Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 May 2022 10:53:20 +0200
Subject: [PATCH 339/359] remove salmon/index modules.json entry

---
 modules.json | 5 +----
 1 file changed, 1 insertion(+), 4 deletions(-)

diff --git a/modules.json b/modules.json
index b1159859..d46c6cfd 100644
--- a/modules.json
+++ b/modules.json
@@ -30,9 +30,6 @@
             "qualimap/rnaseq": {
                 "git_sha": "e20e57f90b6787ac9a010a980cf6ea98bd990046"
             },
-            "salmon/index": {
-                "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
-            },
             "samtools/faidx": {
                 "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773"
             },
@@ -53,4 +50,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}

From 1899d508f2ce42fbaf4ce9b52ce414971ac54a6a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 May 2022 11:54:31 +0200
Subject: [PATCH 340/359] input filtered fusionreport fusions to
 fusioninspector instead of all fusions

---
 subworkflows/local/fusionreport_workflow.nf | 6 ++++--
 workflows/rnafusion.nf                      | 2 +-
 2 files changed, 5 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index b0de9f4a..82f7ba1f 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -25,8 +25,10 @@ workflow FUSIONREPORT_WORKFLOW {
         ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
 
     emit:
-        versions        = ch_versions.ifEmpty(null)
-        fusion_list     = FUSIONREPORT.out.fusion_list
+        versions                 = ch_versions.ifEmpty(null)
+        fusion_list              = FUSIONREPORT.out.fusion_list
+        fusion_list_filtered     = FUSIONREPORT.out.fusion_list_filtered
+
 
 }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 246edf50..586e7cf4 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -203,7 +203,7 @@ workflow RNAFUSION {
     //Run fusionInpector
     FUSIONINSPECTOR_WORKFLOW (
         ch_cat_fastq,
-        FUSIONREPORT_WORKFLOW.out.fusion_list
+        FUSIONREPORT_WORKFLOW.out.fusion_list_filtered
     )
     ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions.first().ifEmpty(null))
 

From 06aeea5210b049c8797e5c59094d1d835870b82b Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Mon, 16 May 2022 10:22:22 +0000
Subject: [PATCH 341/359] Template update for nf-core/tools version 2.4

---
 .github/workflows/awsfulltest.yml     |   3 -
 .github/workflows/awstest.yml         |   3 -
 .github/workflows/branch.yml          |   3 +-
 .github/workflows/ci.yml              |   2 -
 .github/workflows/fix-linting.yml     |  55 ++++++++++
 .github/workflows/linting.yml         |   4 +-
 .github/workflows/linting_comment.yml |   1 -
 .prettierignore                       |   9 ++
 CHANGELOG.md                          |   2 +-
 README.md                             |  25 ++---
 assets/email_template.html            | 142 ++++++++------------------
 bin/check_samplesheet.py              |  16 ++-
 nextflow.config                       |   4 +-
 13 files changed, 137 insertions(+), 132 deletions(-)
 create mode 100644 .github/workflows/fix-linting.yml
 create mode 100644 .prettierignore

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 9b4362ea..c0a06786 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -28,6 +28,3 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
             }
           profiles: test_full,aws_tower
-          nextflow_config: |
-            process.errorStrategy = 'retry'
-            process.maxRetries = 3
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 933cbbf5..5a04305d 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -23,6 +23,3 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-test-${{ github.sha }}"
             }
           profiles: test,aws_tower
-          nextflow_config: |
-            process.errorStrategy = 'retry'
-            process.maxRetries = 3
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 8623dfc2..ccb7211b 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -13,7 +13,7 @@ jobs:
       - name: Check PRs
         if: github.repository == 'nf-core/rnafusion'
         run: |
-          "{ [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]"
+          { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/rnafusion ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
 
       # If the above check failed, post a comment on the PR explaining the failure
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
@@ -42,4 +42,3 @@ jobs:
             Thanks again for your contribution!
           repo-token: ${{ secrets.GITHUB_TOKEN }}
           allow-repeats: false
-#
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 791ad5a0..f54bb8b7 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -48,5 +48,3 @@ jobs:
         # Remember that you can parallelise this by using strategy.matrix
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results
-
-#
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
new file mode 100644
index 00000000..2ffbb6ea
--- /dev/null
+++ b/.github/workflows/fix-linting.yml
@@ -0,0 +1,55 @@
+name: Fix linting from a comment
+on:
+  issue_comment:
+    types: [created]
+
+jobs:
+  deploy:
+    # Only run if comment is on a PR with the main repo, and if it contains the magic keywords
+    if: >
+      contains(github.event.comment.html_url, '/pull/') &&
+      contains(github.event.comment.body, '@nf-core-bot fix linting') &&
+      github.repository == 'nf-core/rnafusion'
+    runs-on: ubuntu-latest
+    steps:
+      # Use the @nf-core-bot token to check out so we can push later
+      - uses: actions/checkout@v3
+        with:
+          token: ${{ secrets.nf_core_bot_auth_token }}
+
+      # Action runs on the issue comment, so we don't get the PR by default
+      # Use the gh cli to check out the PR
+      - name: Checkout Pull Request
+        run: gh pr checkout ${{ github.event.issue.number }}
+        env:
+          GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
+
+      - uses: actions/setup-node@v2
+
+      - name: Install Prettier
+        run: npm install -g prettier @prettier/plugin-php
+
+      # Check that we actually need to fix something
+      - name: Run 'prettier --check'
+        id: prettier_status
+        run: |
+          if prettier --check ${GITHUB_WORKSPACE}; then
+            echo "::set-output name=result::pass"
+          else
+            echo "::set-output name=result::fail"
+          fi
+
+      - name: Run 'prettier --write'
+        if: steps.prettier_status.outputs.result == 'fail'
+        run: prettier --write ${GITHUB_WORKSPACE}
+
+      - name: Commit & push changes
+        if: steps.prettier_status.outputs.result == 'fail'
+        run: |
+          git config user.email "core@nf-co.re"
+          git config user.name "nf-core-bot"
+          git config push.default upstream
+          git add .
+          git status
+          git commit -m "[automated] Fix linting with Prettier"
+          git push
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index e9cf5de3..77358dee 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -48,7 +48,7 @@ jobs:
           wget -qO- get.nextflow.io | bash
           sudo mv nextflow /usr/local/bin/
 
-      - uses: actions/setup-python@v1
+      - uses: actions/setup-python@v3
         with:
           python-version: "3.6"
           architecture: "x64"
@@ -78,5 +78,3 @@ jobs:
             lint_log.txt
             lint_results.md
             PR_number.txt
-
-#
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 91c487a1..04758f61 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -26,4 +26,3 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
           path: linting-logs/lint_results.md
-#
diff --git a/.prettierignore b/.prettierignore
new file mode 100644
index 00000000..d0e7ae58
--- /dev/null
+++ b/.prettierignore
@@ -0,0 +1,9 @@
+email_template.html
+.nextflow*
+work/
+data/
+results/
+.DS_Store
+testing/
+testing*
+*.pyc
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 01d65559..b104b216 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v1.3.0dev - [date]
+## v2.0.0dev - [date]
 
 Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
 
diff --git a/README.md b/README.md
index 465054ef..733c34e9 100644
--- a/README.md
+++ b/README.md
@@ -1,18 +1,19 @@
-# ![nf-core/rnafusion](docs/images/nf-core/rnafusion_logo_light.png#gh-light-mode-only) ![nf-core/rnafusion](docs/images/nf-core/rnafusion_logo_dark.png#gh-dark-mode-only)
+# ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_light.png#gh-light-mode-only) ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_dark.png#gh-dark-mode-only)
 
 [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+CI%22)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)
-[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)
-[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
-
-[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
-[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
-[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
-[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
-
-[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)
-[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)
-[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
+[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)
+[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8)](https://doi.org/10.5281/zenodo.XXXXXXX)
+
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg)](https://www.nextflow.io/)
+[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?logo=anaconda)](https://docs.conda.io/en/latest/)
+[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?logo=docker)](https://www.docker.com/)
+[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg)](https://sylabs.io/docs/)
+[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/rnafusion)
+
+[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?logo=slack)](https://nfcore.slack.com/channels/rnafusion)
+[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?logo=twitter)](https://twitter.com/nf_core)
+[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?logo=youtube)](https://www.youtube.com/c/nf-core)
 
 ## Introduction
 
diff --git a/assets/email_template.html b/assets/email_template.html
index 306f8e4f..efcd0eaa 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -1,111 +1,53 @@
 <html>
-  <head>
-    <meta charset="utf-8" />
-    <meta http-equiv="X-UA-Compatible" content="IE=edge" />
-    <meta name="viewport" content="width=device-width, initial-scale=1" />
+<head>
+  <meta charset="utf-8">
+  <meta http-equiv="X-UA-Compatible" content="IE=edge">
+  <meta name="viewport" content="width=device-width, initial-scale=1">
 
-    <!-- prettier-ignore -->
-    <meta name="description" content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community." />
-    <title>nf-core/rnafusion Pipeline Report</title>
-  </head>
-  <body>
-    <div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto">
-      <img src="cid:nfcorepipelinelogo" />
+  <meta name="description" content="nf-core/rnafusion: Nextflow rnafusion analysis pipeline, part of the nf-core community.">
+  <title>nf-core/rnafusion Pipeline Report</title>
+</head>
+<body>
+<div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto;">
 
-      <h1>nf-core/rnafusion v${version}</h1>
-      <h2>Run Name: $runName</h2>
+<img src="cid:nfcorepipelinelogo">
 
-      <% if (!success){ out << """
-      <div
-        style="
-          color: #a94442;
-          background-color: #f2dede;
-          border-color: #ebccd1;
-          padding: 15px;
-          margin-bottom: 20px;
-          border: 1px solid transparent;
-          border-radius: 4px;
-        "
-      >
-        <h4 style="margin-top: 0; color: inherit">nf-core/rnafusion execution completed unsuccessfully!</h4>
+<h1>nf-core/rnafusion v${version}</h1>
+<h2>Run Name: $runName</h2>
+
+<% if (!success){
+    out << """
+    <div style="color: #a94442; background-color: #f2dede; border-color: #ebccd1; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
+        <h4 style="margin-top:0; color: inherit;">nf-core/rnafusion execution completed unsuccessfully!</h4>
         <p>The exit status of the task that caused the workflow execution to fail was: <code>$exitStatus</code>.</p>
         <p>The full error message was:</p>
-        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0">${errorReport}</pre>
-      </div>
-      """ } else { out << """
-      <div
-        style="
-          color: #3c763d;
-          background-color: #dff0d8;
-          border-color: #d6e9c6;
-          padding: 15px;
-          margin-bottom: 20px;
-          border: 1px solid transparent;
-          border-radius: 4px;
-        "
-      >
+        <pre style="white-space: pre-wrap; overflow: visible; margin-bottom: 0;">${errorReport}</pre>
+    </div>
+    """
+} else {
+    out << """
+    <div style="color: #3c763d; background-color: #dff0d8; border-color: #d6e9c6; padding: 15px; margin-bottom: 20px; border: 1px solid transparent; border-radius: 4px;">
         nf-core/rnafusion execution completed successfully!
-      </div>
-      """ } %>
+    </div>
+    """
+}
+%>
 
-      <p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
-      <p>The command used to launch the workflow was as follows:</p>
-      <pre
-        style="
-          white-space: pre-wrap;
-          overflow: visible;
-          background-color: #ededed;
-          padding: 15px;
-          border-radius: 4px;
-          margin-bottom: 30px;
-        "
-      >
-$commandLine</pre
-      >
+<p>The workflow was completed at <strong>$dateComplete</strong> (duration: <strong>$duration</strong>)</p>
+<p>The command used to launch the workflow was as follows:</p>
+<pre style="white-space: pre-wrap; overflow: visible; background-color: #ededed; padding: 15px; border-radius: 4px; margin-bottom:30px;">$commandLine</pre>
 
-      <h3>Pipeline Configuration:</h3>
-      <table
-        style="
-          width: 100%;
-          max-width: 100%;
-          border-spacing: 0;
-          border-collapse: collapse;
-          border: 0;
-          margin-bottom: 30px;
-        "
-      >
-        <tbody style="border-bottom: 1px solid #ddd">
-          <% out << summary.collect{ k,v -> "
-          <tr>
-            <th
-              style="
-                text-align: left;
-                padding: 8px 0;
-                line-height: 1.42857143;
-                vertical-align: top;
-                border-top: 1px solid #ddd;
-              "
-            >
-              $k
-            </th>
-            <td
-              style="
-                text-align: left;
-                padding: 8px;
-                line-height: 1.42857143;
-                vertical-align: top;
-                border-top: 1px solid #ddd;
-              "
-            >
-              <pre style="white-space: pre-wrap; overflow: visible">$v</pre>
-            </td>
-          </tr>
-          " }.join("\n") %>
-        </tbody>
-      </table>
+<h3>Pipeline Configuration:</h3>
+<table style="width:100%; max-width:100%; border-spacing: 0; border-collapse: collapse; border:0; margin-bottom: 30px;">
+    <tbody style="border-bottom: 1px solid #ddd;">
+        <% out << summary.collect{ k,v -> "<tr><th style='text-align:left; padding: 8px 0; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'>$k</th><td style='text-align:left; padding: 8px; line-height: 1.42857143; vertical-align: top; border-top: 1px solid #ddd;'><pre style='white-space: pre-wrap; overflow: visible;'>$v</pre></td></tr>" }.join("\n") %>
+    </tbody>
+</table>
 
-      <p>nf-core/rnafusion</p>
-      <p><a href="https://github.com/nf-core/rnafusion">https://github.com/nf-core/rnafusion</a></p>
-    </div>
-  </body>
+<p>nf-core/rnafusion</p>
+<p><a href="https://github.com/nf-core/rnafusion">https://github.com/nf-core/rnafusion</a></p>
+
+</div>
+
+</body>
 </html>
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 5473b624..3652c63c 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -98,7 +98,7 @@ def _validate_pair(self, row):
         if row[self._first_col] and row[self._second_col]:
             row[self._single_col] = False
             assert (
-                Path(row[self._first_col]).suffixes == Path(row[self._second_col]).suffixes
+                Path(row[self._first_col]).suffixes[-2:] == Path(row[self._second_col]).suffixes[-2:]
             ), "FASTQ pairs must have the same file extensions."
         else:
             row[self._single_col] = True
@@ -129,6 +129,16 @@ def validate_unique_samples(self):
                     row[self._sample_col] = f"{sample}_T{seen[sample]}"
 
 
+def read_head(handle, num_lines=10):
+    """Read the specified number of lines from the current position in the file."""
+    lines = []
+    for idx, line in enumerate(handle):
+        if idx == num_lines:
+            break
+        lines.append(line)
+    return "".join(lines)
+
+
 def sniff_format(handle):
     """
     Detect the tabular format.
@@ -144,13 +154,13 @@ def sniff_format(handle):
         https://docs.python.org/3/glossary.html#term-text-file
 
     """
-    peek = handle.read(2048)
+    peek = read_head(handle)
+    handle.seek(0)
     sniffer = csv.Sniffer()
     if not sniffer.has_header(peek):
         logger.critical(f"The given sample sheet does not appear to contain a header.")
         sys.exit(1)
     dialect = sniffer.sniff(peek)
-    handle.seek(0)
     return dialect
 
 
diff --git a/nextflow.config b/nextflow.config
index 270f9528..86dd3752 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -159,7 +159,7 @@ trace {
 }
 dag {
     enabled = true
-    file    = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg"
+    file    = "${params.tracedir}/pipeline_dag_${trace_timestamp}.html"
 }
 
 manifest {
@@ -169,7 +169,7 @@ manifest {
     description     = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
     mainScript      = 'main.nf'
     nextflowVersion = '!>=21.10.3'
-    version         = '1.3.0dev'
+    version         = '2.0.0dev'
 }
 
 // Load modules.config for DSL2 module specific options

From 86d32d9d9a144222c6280636c90db9dd890df595 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 10:47:37 +0200
Subject: [PATCH 342/359] correct ci test to actually run pizzly

---
 .github/workflows/ci.yml | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 79189ac9..affe76eb 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -48,10 +48,10 @@ jobs:
           --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly \
           --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
 
-      - name: Test building pizzly references
+      - name: Test running pizzly references
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
           --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly \
-          --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
+          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
 
 #

From e69427e107a0ce037e4873049070c0d5aa6ba8b4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 10:48:54 +0200
Subject: [PATCH 343/359] change samplesheet for test

---
 assets/samplesheet_valid.csv | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/assets/samplesheet_valid.csv b/assets/samplesheet_valid.csv
index 7131bd5d..4ac8f7b1 100644
--- a/assets/samplesheet_valid.csv
+++ b/assets/samplesheet_valid.csv
@@ -1,2 +1,2 @@
 sample,fastq_1,fastq_2,strandedness
-GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse
+test,https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz,https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_2.fq.gz,forward

From fe0983d934a14717224a6ad00dcbb6f435e9c439 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 10:51:41 +0200
Subject: [PATCH 344/359] add possibility to feed either all fusions from
 fusionreport to fusioninspector or just the filtered fusions (>2 tools)

---
 nextflow.config                                | 2 ++
 nextflow_schema.json                           | 5 +++++
 subworkflows/local/fusioninspector_workflow.nf | 4 +++-
 workflows/rnafusion.nf                         | 1 +
 4 files changed, 11 insertions(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 1243d76a..79842bf4 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -28,6 +28,8 @@ params {
     genomes                    = [:]
     starfusion_build           = true
 
+    // Filtering
+    fusioninspector_filter     = false
 
 
     // Alignment options
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 8d68e568..86365c5b 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -134,6 +134,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
+                "fusioninspector_filter": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Feed filtered fusionreport fusions to fusioninspector"
+                },
                 "fusionreport": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index 6c2a9090..4ac83bdd 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -5,12 +5,14 @@ workflow FUSIONINSPECTOR_WORKFLOW {
     take:
         reads
         fusion_list
+        fusion_list_filtered
 
     main:
         ch_versions = Channel.empty()
         index ="${params.starfusion_ref}"
+        ch_fusion_list = params.fusioninspector_filter ? fusion_list_filtered : fusion_list
 
-        FUSIONINSPECTOR( reads, fusion_list , index )
+        FUSIONINSPECTOR( reads, ch_fusion_list , index )
         ch_versions = ch_versions.mix(FUSIONINSPECTOR.out.versions)
 
     emit:
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 586e7cf4..9dac31ce 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -203,6 +203,7 @@ workflow RNAFUSION {
     //Run fusionInpector
     FUSIONINSPECTOR_WORKFLOW (
         ch_cat_fastq,
+        FUSIONREPORT_WORKFLOW.out.fusion_list,
         FUSIONREPORT_WORKFLOW.out.fusion_list_filtered
     )
     ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions.first().ifEmpty(null))

From aee5aeff8362586db2e251590b3084dcb20d33ec Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 10:58:58 +0200
Subject: [PATCH 345/359] update usage

---
 docs/usage.md | 20 ++++++++++++++++----
 1 file changed, 16 insertions(+), 4 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 413ea667..71f5cd94 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -47,20 +47,32 @@ Optional: by default STAR-Fusion references are built. You can also download the
 
 ```bash
 nextflow run nf-core/rnafusion \
---build_references --starfusion \
+--build_references --starfusion/--all \
 --starfusion_build false \
 --genomes_base <PATH>
 ```
 
-### Running the detection tools
+Then use the flag `--starfusion_build` while running the detection.
+
+### Running all detection tools
 
 ```bash
 nextflow run nf-core/rnafusion \
---build_references --all \
+--input '[path to samplesheet file]' --all \
+--outdir <PATH> \
+```
+
+Visualisation tools will be run on all fusions detected.
+
+### Running a specific detection tool
+
+```bash
+nextflow run nf-core/rnafusion \
+--input '[path to samplesheet file]' --<tool> \
 --outdir <PATH> \
 ```
 
-Visualisation tools will be run on all fusion detected.
+Visualisation tools will be run on all fusions detected.
 
 #### Optional manual feed-in of fusion files
 

From 2263355f0b678894794e54b5774258f264e0970b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 15:20:26 +0200
Subject: [PATCH 346/359] gtf generation independently of starfusion

---
 workflows/build_references.nf | 11 +++++++----
 1 file changed, 7 insertions(+), 4 deletions(-)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index ccea93a1..c23466bd 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -50,14 +50,17 @@ workflow BUILD_REFERENCES {
     if (params.starfusion || params.all) {
         if (params.starfusion_build){
             STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
-            GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
-        }
-        else {
+        } else {
             STARFUSION_DOWNLOAD()
-            GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.chrgtf)
         }
     }
 
+    if (params.starfusion_build){
+        GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
+    } else {
+        GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.chrgtf)
+    }
+
     if (params.fusionreport || params.all) {
         FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
     }

From d86149da0430258292e8dca8d92f2779bccb3a19 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 15:39:26 +0200
Subject: [PATCH 347/359] update ci test to download fusionreport db

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index c8c4bb0b..bc9aa997 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -45,7 +45,7 @@ jobs:
       - name: Test building pizzly references
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
-          --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly \
+          --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly --fusionreport\
           --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
 
       - name: Test running pizzly references

From 6be89a83e1d34904f0eab51ed83f2a76c6623840 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 16:08:21 +0200
Subject: [PATCH 348/359] extra space

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index bc9aa997..723462fb 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -45,7 +45,7 @@ jobs:
       - name: Test building pizzly references
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
-          --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly --fusionreport\
+          --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly --fusionreport \
           --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
 
       - name: Test running pizzly references

From bd183b5ff43530f44b1a1a3b8145e78c2db185a7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 16:14:30 +0200
Subject: [PATCH 349/359] use github secret for cosmic database

---
 .github/workflows/ci.yml | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 723462fb..14feca9a 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -46,7 +46,8 @@ jobs:
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
           --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly --fusionreport \
-          --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
+          --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references \
+          --cosmic_username secrets.COSMIC_USERNAME --cosmic_passwd secrets.COSMIC_PASSWD
 
       - name: Test running pizzly references
         run: |

From faa32136292ca1cdb0039493af805c61f9a015a9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 16:37:13 +0200
Subject: [PATCH 350/359] update secret formatting

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 14feca9a..405bf72b 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -47,7 +47,7 @@ jobs:
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
           --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly --fusionreport \
           --build_references --genomes_base /home/runner/work/rnafusion/rnafusion/results/references \
-          --cosmic_username secrets.COSMIC_USERNAME --cosmic_passwd secrets.COSMIC_PASSWD
+          --cosmic_username ${{ secrets.COSMIC_USERNAME }} --cosmic_passwd ${{ secrets.COSMIC_PASSWD }}
 
       - name: Test running pizzly references
         run: |

From 70826c4fdcd6f4bc4a3e2dde70ba096cea1368a5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 May 2022 17:10:54 +0200
Subject: [PATCH 351/359] use fusioninspector stub in ci test

---
 .github/workflows/ci.yml              | 2 +-
 modules/local/fusioninspector/main.nf | 6 ++++++
 2 files changed, 7 insertions(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 405bf72b..750af29d 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -53,4 +53,4 @@ jobs:
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker \
           --outdir /home/runner/work/rnafusion/rnafusion/results --pizzly \
-          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
+          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references -stub
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 8f2df0ac..c5fbe360 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -40,4 +40,10 @@ process FUSIONINSPECTOR {
         STAR-Fusion: \$(echo STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
     END_VERSIONS
     """
+
+    stub:
+    """
+    touch versions.yml
+    touch FusionInspector.log
+    """
 }

From 80057289d07728c968fa22a0ae6daa23a3d02da5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 May 2022 11:48:21 +0200
Subject: [PATCH 352/359] use samplesheet from nf-core/test-dataset for testing

---
 conf/test.config      | 2 +-
 conf/test_full.config | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index c222f219..35910107 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -20,6 +20,6 @@ params {
     max_time   = 6.h
 
     // Input data
-    input            = 'assets/samplesheet_valid.csv'
+    input            = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
 
 }
diff --git a/conf/test_full.config b/conf/test_full.config
index 6309067d..5525cad2 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -16,7 +16,7 @@ params {
 
     // Input data
     genome                 = 'GRCh38'
-    input                  = 'assets/samplesheet.csv'
+    input                  = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
     genomes_base           = "${params.outdir}/references"
     all                    = true
 

From 65faa08bbf6a8c3fd5f4e512cfb6f128e3fda8ce Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 May 2022 15:09:19 +0200
Subject: [PATCH 353/359] update authorships

---
 README.md       | 8 +++++---
 nextflow.config | 2 +-
 2 files changed, 6 insertions(+), 4 deletions(-)

diff --git a/README.md b/README.md
index fae1c8ee..5f705cb4 100644
--- a/README.md
+++ b/README.md
@@ -120,12 +120,14 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline [usag
 
 ## Credits
 
-nf-core/rnafusion was originally written by Martin Proks ([@matq007](https://github.com/matq007)), Praveen Raj ([@praveenraj2018](https://github.com/praveenraj2018))
+nf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), and extended by Annick Renevey ([@rannick](https://github.com/rannick))
 
 We thank the following people for their **extensive assistance** in the development of this pipeline:
 
-- Annick Renevey ([@rannick](https://github.com/rannick))
-
+- [Phil Ewels](https://github.com/ewels)
+- [Maxime Garcia](https://github.com/maxulysse)
+- [Rickard Hammarén](https://github.com/Hammarn)
+- [Praveen Raj](https://github.com/praveenraj2018)
 ## Contributions and Support
 
 If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).
diff --git a/nextflow.config b/nextflow.config
index be0a361c..9d8fadc7 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -208,7 +208,7 @@ dag {
 
 manifest {
     name            = 'nf-core/rnafusion'
-    author          = 'Martin Proks, @praveenraj2018'
+    author          = 'Martin Proks, Annick Renevey'
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
     mainScript      = 'main.nf'

From 3f119a8359808d41f6af34f1c1df97bb9de1b13e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 May 2022 09:37:29 +0200
Subject: [PATCH 354/359] prettier lint

---
 README.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/README.md b/README.md
index 5f705cb4..601517e0 100644
--- a/README.md
+++ b/README.md
@@ -128,6 +128,7 @@ We thank the following people for their **extensive assistance** in the developm
 - [Maxime Garcia](https://github.com/maxulysse)
 - [Rickard Hammarén](https://github.com/Hammarn)
 - [Praveen Raj](https://github.com/praveenraj2018)
+
 ## Contributions and Support
 
 If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).

From abc2597efd11c83a0c493895c57a5c0274ce9fe7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 May 2022 09:45:16 +0200
Subject: [PATCH 355/359] replace LICENSE with template

---
 LICENSE | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/LICENSE b/LICENSE
index 8a50c49d..86e71fe1 100644
--- a/LICENSE
+++ b/LICENSE
@@ -1,6 +1,6 @@
 MIT License
 
-Copyright (c) Martin Proks, @praveenraj2018
+Copyright (c) Martin Proks, Annick Renevey
 
 Permission is hereby granted, free of charge, to any person obtaining a copy
 of this software and associated documentation files (the "Software"), to deal

From 1d8c86b4e93c1d222af56cdfa4e247ef6dffebb5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 May 2022 10:16:16 +0200
Subject: [PATCH 356/359] modify authorships

---
 README.md       | 6 +++---
 nextflow.config | 2 +-
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/README.md b/README.md
index 601517e0..fa46e489 100644
--- a/README.md
+++ b/README.md
@@ -120,13 +120,13 @@ The nf-core/rnafusion pipeline comes with documentation about the pipeline [usag
 
 ## Credits
 
-nf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), and extended by Annick Renevey ([@rannick](https://github.com/rannick))
+nf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))
 
-We thank the following people for their **extensive assistance** in the development of this pipeline:
+We thank the following people for their help in the development of this pipeline:
 
 - [Phil Ewels](https://github.com/ewels)
-- [Maxime Garcia](https://github.com/maxulysse)
 - [Rickard Hammarén](https://github.com/Hammarn)
+- [Alexander Peltzer](https://github.com/apeltzer)
 - [Praveen Raj](https://github.com/praveenraj2018)
 
 ## Contributions and Support
diff --git a/nextflow.config b/nextflow.config
index 9d8fadc7..b8c090dd 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -208,7 +208,7 @@ dag {
 
 manifest {
     name            = 'nf-core/rnafusion'
-    author          = 'Martin Proks, Annick Renevey'
+    author          = 'Martin Proks, Maxime Garcia, Annick Renevey, Rickard Hammarén, Phil Ewels'
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
     mainScript      = 'main.nf'

From 40ce163acb8d2d3bdaae9341b9469003e046ec35 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 May 2022 10:16:52 +0200
Subject: [PATCH 357/359] modify LICENSE

---
 LICENSE | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/LICENSE b/LICENSE
index 86e71fe1..a12d4a75 100644
--- a/LICENSE
+++ b/LICENSE
@@ -1,6 +1,6 @@
 MIT License
 
-Copyright (c) Martin Proks, Annick Renevey
+Copyright (c) Martin Proks, Maxime Garcia, Annick Renevey, Rickard Hammarén, Phil Ewels
 
 Permission is hereby granted, free of charge, to any person obtaining a copy
 of this software and associated documentation files (the "Software"), to deal

From 671c320e29a9e7851ee2a0e533c083022cebdcbd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 May 2022 12:51:42 +0200
Subject: [PATCH 358/359] update authorship

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index b8c090dd..9d8fadc7 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -208,7 +208,7 @@ dag {
 
 manifest {
     name            = 'nf-core/rnafusion'
-    author          = 'Martin Proks, Maxime Garcia, Annick Renevey, Rickard Hammarén, Phil Ewels'
+    author          = 'Martin Proks, Annick Renevey'
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = 'Nextflow rnafusion analysis pipeline, part of the nf-core community.'
     mainScript      = 'main.nf'

From 7aeb7d733ef426d5cf66ed18d621ca02f6ae8991 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 May 2022 12:56:32 +0200
Subject: [PATCH 359/359] update LICENSE

---
 LICENSE | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/LICENSE b/LICENSE
index a12d4a75..86e71fe1 100644
--- a/LICENSE
+++ b/LICENSE
@@ -1,6 +1,6 @@
 MIT License
 
-Copyright (c) Martin Proks, Maxime Garcia, Annick Renevey, Rickard Hammarén, Phil Ewels
+Copyright (c) Martin Proks, Annick Renevey
 
 Permission is hereby granted, free of charge, to any person obtaining a copy
 of this software and associated documentation files (the "Software"), to deal