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Error when converting h5Seurat to h5ad #162

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zoan128 opened this issue Aug 31, 2023 · 11 comments
Open

Error when converting h5Seurat to h5ad #162

zoan128 opened this issue Aug 31, 2023 · 11 comments

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@zoan128
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zoan128 commented Aug 31, 2023

Convert("I:/result/csph-skuh/csph-skuh/csph.h5Seurat",overwrite = T,dest = "h5ad")

Validating h5Seurat file
Adding data from RNA as X
Error in assay.group$obj_copy_to(dst_loc = dfile, dst_name = "X", src_name = x.data) :
HDF5-API Errors:
error #000: D:/a/rtools-packages/rtools-packages/mingw-w64-hdf5/src/hdf5-1.8.16/src/H5Ocopy.c in H5Ocopy(): line 259: source object not found
class: HDF5
major: Symbol table
minor: Object not found
error #1: D:/a/rtools-packages/rtools-packages/mingw-w64-hdf5/src/hdf5-1.8.16/src/H5Gloc.c in H5G_loc_find(): line 430: can't find object
class: HDF5
major: Symbol table
minor: Object not found
error #2: D:/a/rtools-packages/rtools-packages/mingw-w64-hdf5/src/hdf5-1.8.16/src/H5Gtraverse.c in H5G_traverse(): line 861: internal path traversal failed
class: HDF5
major: Symbol table
minor: Object not found
error #3: D:/a/rtools-packages/rtools-packages/mingw-w64-hdf5/src/hdf5-1.8.16/src/H5Gtraverse.c in H5G_traverse_real(): line 641: traversal operator failed
class: HDF5
major: Symbol table
minor: Callback failed
error #4: D:/a/rtools-packages/

@jayypaul
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I also have this issue. I'm thinking it's v5 related?

@CeciPR
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CeciPR commented Jan 2, 2024

I have the same error!

@LeaLe88
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LeaLe88 commented Jan 16, 2024

Same here!

@zoan128
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zoan128 commented Jan 16, 2024

I haven't reslove the problem, but the files can be converted by another tool named SCP.
https://link.zhihu.com/?target=https%3A//github.com/zhanghao-njmu/SCP

@CeciPR
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CeciPR commented Jan 16, 2024 via email

@LeaLe88
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LeaLe88 commented Jan 16, 2024

Thank you

@LorenzoMerotto
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Same issue here, even though I don't have Seurat v5

@thoalaubodah
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thoalaubodah commented Jan 31, 2024

I also have this issue with a Seurat v5 object.

@zyzy-98
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zyzy-98 commented Jan 31, 2024

Not using Seurat V5, but I do have this issue as well.

@matejasoretic
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same issue here, on Seurat v4

@Nicomakii
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Hey all, I found the solution here, you have to convert Seurat V5 back to V3 first

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