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FANC (pronounced "fancy") is the Female Adult Nerve Cord, a GridTape-TEM dataset of an adult Drosophila melanogaster's ventral nerve cord. The EM dataset was first published in Phelps, Hildebrand, Graham et al. 2021 Cell, after which we applied automated methods for reconstructing neurons, synapses, and nuclei to accelerate reconstruction of the ventral nerve cord connectome, as described in Connectomic reconstruction of a female Drosophila ventral nerve cord (Azevedo, Lesser, Phelps, Mark et al. 2024 Nature). We now oversee a collaborative effort including over 20 labs to finish the FANC connectome by manually correcting the remaining errors in the automated reconstructions. As of April 2024, about half of the neurons in FANC have been proofread.

For more information, view this repository's README and read the sections below.

Collaborative connectome reconstruction community

Community managed by

• Jasper Phelps: [email protected] / Website / GitHub
• Wei-Chung Allen Lee: [email protected] / Website / GitHub

Table of contents

• Joining the community
• Community Code of Conduct
• Getting started in the FANC community
• Neuroglancer states for proofreading
• Neuron annotations
• Connectome analysis tools

Publications and public data releases

Below is a list of publications that include data from FANC. Many of these publications include public data releases, which can be accessed through the links below.

Manual reconstructions (2021-2022)

Manual skeleton reconstructions of neurons and synapses performed in CATMAID. These are all hosted by Virtual Fly Brain at https://catmaid.virtualflybrain.org/

• Reconstruction of motor control circuits in adult Drosophila using automated transmission electron microscopy (Phelps, Hildebrand, Graham et al. 2021 Cell)
• Functional architecture of neural circuits for leg proprioception in Drosophila (Chen et al. 2021 Current Biology)
• Descending neuron population dynamics during odor‐evoked and spontaneous limb‐dependent behaviors (Aymanns, Chen, & Ramdya 2022 eLife)

Automated reconstructions (2022-present)

Most of the data accompanying each publication below is being deposited into the Google Cloud Storage bucket gs://lee-lab_female-adult-nerve-cord. View the storage bucket in your browser, or click on each paper below for more information.

Listed in chronological order by preprint date, oldest at the top to newest at the bottom:

• Connectomic reconstruction of a female Drosophila ventral nerve cord (Azevedo, Lesser, Phelps, Mark et al. 2024 Nature)
• Synaptic architecture of leg and wing premotor control networks in Drosophila (Lesser, Azevedo, et al. 2024 Nature)
• Organization of an Ascending Circuit that Conveys Flight Motor State (Cheong, Boone, Bennett et al. 2024 Curr Biol)
• Neural circuit mechanisms underlying context‐specific halting in Drosophila (Sapkal et al. 2023 bioRxiv)
• Fine‐grained descending control of steering in walking Drosophila (Yang et al. 2023 bioRxiv)
• Presynaptic inhibition selectively suppresses leg proprioception in behaving Drosophila (Dallmann et al. 2023 bioRxiv)
• Mechanosensory and command contributions to the Drosophila grooming sequence (Yoshikawa et al. 2024 Current Biology)
• Divergent neural circuits for proprioceptive and exteroceptive sensing of the Drosophila leg (Lee et al. 2024 bioRxiv)
• Comparative connectomics of the descending and ascending neurons of the Drosophila nervous system: stereotypy and sexual dimorphism (Stürner, Brooks et al. 2024 bioRxiv)
• Inhibitory circuits coordinate leg movements during Drosophila grooming (Syed et al. 2024 bioRxiv)