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about validation set used in your paper #19

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xieyy46 opened this issue May 24, 2022 · 2 comments
Open

about validation set used in your paper #19

xieyy46 opened this issue May 24, 2022 · 2 comments

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@xieyy46
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xieyy46 commented May 24, 2022

Hi nanom6a team! I noticed that you used m6A sites identified by miCLIP from public data (Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification) in Fig.3a of your paper, and the number of m6A sites identified by miCLIP is 4685, however in paper Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification that numbr is 93046. I'd want to know how you got 4685 m6A sites from 93046 site?
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@gaoyubang
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I fillter m6A sites with RRACH motif

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@xieyy46
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xieyy46 commented May 24, 2022

Hi yubang! Your script (seq=loader.fetch(chro,pos-5,pos+5)) meant that you will save sites if the flanking 5bp (11bp in total) contains RRACH, though the flanking 2bp (5bp in total) may not == RRACH?

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