diff --git a/lib/galaxy/dependencies/dev-requirements.txt b/lib/galaxy/dependencies/dev-requirements.txt
index a0b0f68801f0..685b1149cd08 100644
--- a/lib/galaxy/dependencies/dev-requirements.txt
+++ b/lib/galaxy/dependencies/dev-requirements.txt
@@ -1,5 +1,5 @@
 # This file was autogenerated by uv via the following command:
-#    uv export --no-hashes --only-group=dev
+#    uv export --frozen --no-hashes --only-group=dev
 aiohappyeyeballs==2.4.3
 aiohttp==3.10.10
 aiosignal==1.3.1
diff --git a/lib/galaxy/dependencies/pinned-requirements.txt b/lib/galaxy/dependencies/pinned-requirements.txt
index 6227984653a6..6f4c5cc99496 100644
--- a/lib/galaxy/dependencies/pinned-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-requirements.txt
@@ -1,5 +1,5 @@
 # This file was autogenerated by uv via the following command:
-#    uv export --no-hashes --no-dev
+#    uv export --frozen --no-hashes --no-dev
 a2wsgi==1.10.7
 adal==1.2.7
 aiobotocore==2.15.2
diff --git a/lib/galaxy/dependencies/pinned-typecheck-requirements.txt b/lib/galaxy/dependencies/pinned-typecheck-requirements.txt
index a835c64276fa..316819f75cd7 100644
--- a/lib/galaxy/dependencies/pinned-typecheck-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-typecheck-requirements.txt
@@ -1,5 +1,5 @@
 # This file was autogenerated by uv via the following command:
-#    uv export --no-hashes --only-group=typecheck
+#    uv export --frozen --no-hashes --only-group=typecheck
 annotated-types==0.7.0
 boto3-stubs==1.35.49
 botocore-stubs==1.35.49
diff --git a/lib/galaxy/dependencies/update.sh b/lib/galaxy/dependencies/update.sh
index 2d26f6b40bef..12078ddbed3d 100755
--- a/lib/galaxy/dependencies/update.sh
+++ b/lib/galaxy/dependencies/update.sh
@@ -42,6 +42,6 @@ else
 fi
 
 # Update pinned requirements files.
-${uv} export --no-hashes --no-dev > "$this_directory/pinned-requirements.txt"
-${uv} export --no-hashes --only-group=dev > "$this_directory/dev-requirements.txt"
-${uv} export --no-hashes --only-group=typecheck > "$this_directory/pinned-typecheck-requirements.txt"
+${uv} export --frozen --no-hashes --no-dev > "$this_directory/pinned-requirements.txt"
+${uv} export --frozen --no-hashes --only-group=dev > "$this_directory/dev-requirements.txt"
+${uv} export --frozen --no-hashes --only-group=typecheck > "$this_directory/pinned-typecheck-requirements.txt"