diff --git a/crates/uv-resolver/src/prerelease.rs b/crates/uv-resolver/src/prerelease.rs
index c452858775f1e..c207186d0a9dc 100644
--- a/crates/uv-resolver/src/prerelease.rs
+++ b/crates/uv-resolver/src/prerelease.rs
@@ -1,9 +1,9 @@
 use uv_pypi_types::RequirementSource;
 
-use uv_normalize::PackageName;
-
 use crate::resolver::ForkSet;
 use crate::{DependencyMode, Manifest, ResolverMarkers};
+use uv_normalize::PackageName;
+use uv_pep440::Operator;
 
 #[derive(Debug, Default, Clone, Copy, PartialEq, Eq, serde::Serialize, serde::Deserialize)]
 #[serde(deny_unknown_fields, rename_all = "kebab-case")]
@@ -84,6 +84,9 @@ impl PrereleaseStrategy {
 
                     if specifier
                         .iter()
+                        .filter(|spec| {
+                            !matches!(spec.operator(), Operator::NotEqual | Operator::NotEqualStar)
+                        })
                         .any(uv_pep440::VersionSpecifier::any_prerelease)
                     {
                         packages.add(&requirement, ());
diff --git a/crates/uv/tests/it/pip_compile.rs b/crates/uv/tests/it/pip_compile.rs
index 5f81f522c4dd3..2e8fbd82a6f55 100644
--- a/crates/uv/tests/it/pip_compile.rs
+++ b/crates/uv/tests/it/pip_compile.rs
@@ -12720,3 +12720,39 @@ fn unsupported_requires_python_dynamic_metadata() -> Result<()> {
 
     Ok(())
 }
+
+#[test]
+fn negation_not_imply_prerelease() -> Result<()> {
+    let context = TestContext::new("3.12");
+
+    let requirements_in = context.temp_dir.child("requirements.in");
+    requirements_in.write_str("flask<2.0.1, !=2.0.0rc1")?;
+    uv_snapshot!(context
+        .pip_compile()
+        .arg("requirements.in"), @r###"
+    success: true
+    exit_code: 0
+    ----- stdout -----
+    # This file was autogenerated by uv via the following command:
+    #    uv pip compile --cache-dir [CACHE_DIR] requirements.in
+    click==8.1.7
+        # via flask
+    flask==2.0.0
+        # via -r requirements.in
+    itsdangerous==2.1.2
+        # via flask
+    jinja2==3.1.3
+        # via flask
+    markupsafe==2.1.5
+        # via
+        #   jinja2
+        #   werkzeug
+    werkzeug==3.0.1
+        # via flask
+
+    ----- stderr -----
+    Resolved 6 packages in [TIME]
+    "###);
+
+    Ok(())
+}