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samblaster not found #161

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CeciliaDeng opened this issue Oct 22, 2024 · 1 comment
Open

samblaster not found #161

CeciliaDeng opened this issue Oct 22, 2024 · 1 comment
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@CeciliaDeng
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Description of the bug

Hi @GallVp , AssemblyQC successfully completed assessing an assembly with HiC data. When I check the HiC QC report, it mentioned [forward/reverse] Hi-C reads not found, as well as 'samblaster command not found'.

image
image

Is somehow samblaster not loaded?
Thank you.

Command used and terminal output

sbatch pfr_assemblyqc
Submitted batch job 7756409

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@CeciliaDeng CeciliaDeng added the bug Something isn't working label Oct 22, 2024
@GallVp GallVp self-assigned this Oct 22, 2024
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GallVp commented Oct 22, 2024

Thank you for submitting an issue on this. I have looked at the report for the pipeline test data and it also has the same items missing.

I think this information is missing because we don't provide the path to the raw reads to the HICQC module. I'll investigate and see if this can be fixed.

Nonetheless, if Subjective Hi-C library judgment is given along with other statistics that means that HICQC had enough information to reach a judgement on the quality of the library. See the following link for interpreting the judgement: https://phasegenomics.github.io/2019/09/19/hic-alignment-and-qc.html

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