diff --git a/api/src/opentrons/protocol_engine/commands/thermocycler/run_extended_profile.py b/api/src/opentrons/protocol_engine/commands/thermocycler/run_extended_profile.py
index 007d169278e4..3cf8a67bf414 100644
--- a/api/src/opentrons/protocol_engine/commands/thermocycler/run_extended_profile.py
+++ b/api/src/opentrons/protocol_engine/commands/thermocycler/run_extended_profile.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python3
-
 """Command models to execute a Thermocycler profile."""
 from __future__ import annotations
 from typing import List, Optional, TYPE_CHECKING, overload, Union