diff --git a/api/src/opentrons/protocol_api/core/engine/protocol.py b/api/src/opentrons/protocol_api/core/engine/protocol.py
index dac8bc44a5b..aa04ffce993 100644
--- a/api/src/opentrons/protocol_api/core/engine/protocol.py
+++ b/api/src/opentrons/protocol_api/core/engine/protocol.py
@@ -228,6 +228,7 @@ def load_labware(
             )
         )
         # FIXME(jbl, 2023-08-14) validating after loading the object issue
+        validation.ensure_labware_is_loadable(load_result.definition, load_location)
         validation.ensure_definition_is_labware(load_result.definition)
 
         # FIXME(mm, 2023-02-21):
diff --git a/api/src/opentrons/protocol_api/validation.py b/api/src/opentrons/protocol_api/validation.py
index 630211e9ac6..a9e595fd204 100644
--- a/api/src/opentrons/protocol_api/validation.py
+++ b/api/src/opentrons/protocol_api/validation.py
@@ -17,7 +17,7 @@
 from opentrons_shared_data.labware.labware_definition import LabwareRole
 from opentrons_shared_data.pipette.types import PipetteNameType
 from opentrons_shared_data.robot.types import RobotType
-
+from opentrons.protocol_engine.types import LabwareLocation, NonStackedLocation
 from opentrons.protocols.api_support.types import APIVersion, ThermocyclerStep
 from opentrons.protocols.api_support.util import APIVersionError
 from opentrons.protocols.models import LabwareDefinition
@@ -83,6 +83,10 @@ class LabwareDefinitionIsNotLabwareError(ValueError):
     """An error raised when a labware is not loaded using `load_labware`."""
 
 
+class LabwareDefinitionIsNotLoadableOnPosition(ValueError):
+    """An error raised when a labware is not able to be loaded at the location."""
+
+
 class InvalidTrashBinLocationError(ValueError):
     """An error raised when attempting to load trash bins in invalid slots."""
 
@@ -253,6 +257,20 @@ def ensure_definition_is_labware(definition: LabwareDefinition) -> None:
         )
 
 
+def ensure_labware_is_loadable(
+    definition: LabwareDefinition, location: LabwareLocation
+) -> None:
+    """Ensure that one of the definition's allowed roles is `stackableOnly` and that the location is a `NonStackedLocation`."""
+    if (
+        definition.allowedRoles
+        and LabwareRole.stackableOnly in definition.allowedRoles
+        and isinstance(location, NonStackedLocation)
+    ):
+        raise LabwareDefinitionIsNotLoadableOnPosition(
+            f"Labware {definition.parameters.loadName} cannot be loaded on {location}."
+        )
+
+
 _MODULE_ALIASES: Dict[str, ModuleModel] = {
     "magdeck": MagneticModuleModel.MAGNETIC_V1,
     "magnetic module": MagneticModuleModel.MAGNETIC_V1,
diff --git a/shared-data/labware/definitions/2/nest_96_wellplate_2ml_deep/2.json b/shared-data/labware/definitions/2/nest_96_wellplate_2ml_deep/2.json
index 775596e7386..38a7dfbe1af 100644
--- a/shared-data/labware/definitions/2/nest_96_wellplate_2ml_deep/2.json
+++ b/shared-data/labware/definitions/2/nest_96_wellplate_2ml_deep/2.json
@@ -1130,6 +1130,11 @@
       "x": 0,
       "y": 0,
       "z": 16.1
+    },
+    "evo_flex_96_tiprack_adapter": {
+      "x": 0,
+      "y": 0,
+      "z": 102
     }
   },
   "stackingOffsetWithModule": {
diff --git a/shared-data/labware/definitions/2/opentrons_evo_96_wellplate_300ul/1.json b/shared-data/labware/definitions/2/opentrons_evo_96_wellplate_300ul/1.json
new file mode 100644
index 00000000000..2530efe5fdb
--- /dev/null
+++ b/shared-data/labware/definitions/2/opentrons_evo_96_wellplate_300ul/1.json
@@ -0,0 +1,1025 @@
+{
+  "ordering": [
+    ["A1", "B1", "C1", "D1", "E1", "F1", "G1", "H1"],
+    ["A2", "B2", "C2", "D2", "E2", "F2", "G2", "H2"],
+    ["A3", "B3", "C3", "D3", "E3", "F3", "G3", "H3"],
+    ["A4", "B4", "C4", "D4", "E4", "F4", "G4", "H4"],
+    ["A5", "B5", "C5", "D5", "E5", "F5", "G5", "H5"],
+    ["A6", "B6", "C6", "D6", "E6", "F6", "G6", "H6"],
+    ["A7", "B7", "C7", "D7", "E7", "F7", "G7", "H7"],
+    ["A8", "B8", "C8", "D8", "E8", "F8", "G8", "H8"],
+    ["A9", "B9", "C9", "D9", "E9", "F9", "G9", "H9"],
+    ["A10", "B10", "C10", "D10", "E10", "F10", "G10", "H10"],
+    ["A11", "B11", "C11", "D11", "E11", "F11", "G11", "H11"],
+    ["A12", "B12", "C12", "D12", "E12", "F12", "G12", "H12"]
+  ],
+  "brand": {
+    "brand": "opentrons_evo",
+    "brandId": []
+  },
+  "metadata": {
+    "displayName": "Opentrons_evo 96 Well Plate 300 µL",
+    "displayCategory": "wellPlate",
+    "displayVolumeUnits": "µL",
+    "tags": []
+  },
+  "dimensions": {
+    "xDimension": 127.75,
+    "yDimension": 86,
+    "zDimension": 34
+  },
+  "wells": {
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+  },
+  "groups": [
+    {
+      "metadata": {
+        "wellBottomShape": "v"
+      },
+      "wells": [
+        "A1",
+        "B1",
+        "C1",
+        "D1",
+        "E1",
+        "F1",
+        "G1",
+        "H1",
+        "A2",
+        "B2",
+        "C2",
+        "D2",
+        "E2",
+        "F2",
+        "G2",
+        "H2",
+        "A3",
+        "B3",
+        "C3",
+        "D3",
+        "E3",
+        "F3",
+        "G3",
+        "H3",
+        "A4",
+        "B4",
+        "C4",
+        "D4",
+        "E4",
+        "F4",
+        "G4",
+        "H4",
+        "A5",
+        "B5",
+        "C5",
+        "D5",
+        "E5",
+        "F5",
+        "G5",
+        "H5",
+        "A6",
+        "B6",
+        "C6",
+        "D6",
+        "E6",
+        "F6",
+        "G6",
+        "H6",
+        "A7",
+        "B7",
+        "C7",
+        "D7",
+        "E7",
+        "F7",
+        "G7",
+        "H7",
+        "A8",
+        "B8",
+        "C8",
+        "D8",
+        "E8",
+        "F8",
+        "G8",
+        "H8",
+        "A9",
+        "B9",
+        "C9",
+        "D9",
+        "E9",
+        "F9",
+        "G9",
+        "H9",
+        "A10",
+        "B10",
+        "C10",
+        "D10",
+        "E10",
+        "F10",
+        "G10",
+        "H10",
+        "A11",
+        "B11",
+        "C11",
+        "D11",
+        "E11",
+        "F11",
+        "G11",
+        "H11",
+        "A12",
+        "B12",
+        "C12",
+        "D12",
+        "E12",
+        "F12",
+        "G12",
+        "H12"
+      ]
+    }
+  ],
+  "parameters": {
+    "format": "96Standard",
+    "quirks": [],
+    "isTiprack": false,
+    "isMagneticModuleCompatible": false,
+    "loadName": "opentrons_evo_96_wellplate_300ul"
+  },
+  "namespace": "opentrons",
+  "allowedRoles": ["stackableOnly"],
+  "version": 1,
+  "schemaVersion": 2,
+  "cornerOffsetFromSlot": {
+    "x": 0,
+    "y": 0,
+    "z": 0
+  },
+  "stackingOffsetWithLabware": {
+    "nest_96_wellplate_2ml_deep": {
+      "x": 0,
+      "y": 0,
+      "z": 20
+    }
+  }
+}
diff --git a/shared-data/python/opentrons_shared_data/labware/labware_definition.py b/shared-data/python/opentrons_shared_data/labware/labware_definition.py
index 3363c874c55..92f76a930b9 100644
--- a/shared-data/python/opentrons_shared_data/labware/labware_definition.py
+++ b/shared-data/python/opentrons_shared_data/labware/labware_definition.py
@@ -125,6 +125,7 @@ class LabwareRole(str, Enum):
     fixture = "fixture"
     adapter = "adapter"
     maintenance = "maintenance"
+    stackableOnly = "stackableOnly"
     lid = "lid"