From 04842958a19a53dc7ee054c98ebd686c2090e1ac Mon Sep 17 00:00:00 2001 From: Josh McVey Date: Fri, 4 Oct 2024 14:57:43 -0500 Subject: [PATCH] test(analyses): 8.0.0 testing protocols into analyses battery (#16407) # Overview Get these into edge ASAP! - [x] Josh reviewed the snapshots PR making sure that protocols with **X** had errors in the snapshot and **S** do not. Not much else to review. We need these in so that PRs to edge are also running against these snapshots. --------- Co-authored-by: github-actions[bot] <41898282+github-actions[bot]@users.noreply.github.com> Co-authored-by: y3rsh <502770+y3rsh@users.noreply.github.com> --- analyses-snapshot-testing/Makefile | 23 + analyses-snapshot-testing/README.md | 20 + .../automation/data/protocols.py | 81 + .../data/protocols_with_overrides.py | 69 + .../citools/Dockerfile.server | 31 + .../protocols/Flex_S_v2_20_1000M_Simple.py | 93 + ...S_v2_20_8_None_PARTIAL_COLUMN_HappyPath.py | 109 + .../Flex_S_v2_20_8_None_SINGLE_HappyPath.py | 131 + ...S_v2_20_8_None_SINGLE_TubeRackCollision.py | 65 + .../Flex_S_v2_20_8_PARTIAL_COLUMN_Overhang.py | 150 + .../Flex_S_v2_20_8_TipsDontMatchWells.py | 87 + .../protocols/Flex_S_v2_20_96_AllCorners.py | 296 + .../files/protocols/Flex_S_v2_20_96_LLD.py | 93 + .../Flex_S_v2_20_96_None_COLUMN_HappyPath.py | 121 + .../Flex_S_v2_20_96_None_Column3_SINGLE_.py | 118 + .../Flex_S_v2_20_96_None_ROW_HappyPath.py | 121 + ...lex_S_v2_20_96_None_SINGLE_4Corners50ul.py | 66 + ...v2_20_96_None_SINGLE_HappyPathNorthSide.py | 143 + ...v2_20_96_None_SINGLE_HappyPathSouthSide.py | 143 + ..._v2_20_96_None_SINGLE_TubeRackCollision.py | 68 + .../protocols/Flex_S_v2_20_96_Reservoir.py | 91 + .../files/protocols/Flex_S_v2_20_P50_LPD.py | 122 + .../Flex_S_v2_20_P50_LPD_wet_tip_scenarios.py | 169 + .../Flex_S_v2_20_P50_touch_tip_directly.py | 66 + .../protocols/Flex_S_v2_20_P8X1000_P50_LLD.py | 106 + ..._v2_20_96_None_Overrides_TooTallLabware.py | 504 + ...v2_20_96_and_8_Overrides_InvalidConfigs.py | 185 + ...e_PARTIAL_COLUMN_HappyPathMixedTipRacks.py | 111 + .../OT2_S_v2_20_8_None_SINGLE_HappyPath.py | 135 + ...S_v2_20_8_None_SINGLE_TubeRackCollision.py | 64 + ...T2_S_v2_20_P20M_PARTIAL_COLUMN_Overhang.py | 155 + .../protocols/OT2_S_v2_20_P20M_Simple.py | 90 + ...2_S_v2_20_P300M_PARTIAL_COLUMN_Overhang.py | 151 + .../protocols/OT2_S_v2_20_P300M_Simple.py | 94 + .../protocols/OT2_S_v2_20_P50_touch_tip.py | 52 + .../OT2_X_v2_20_8_Overrides_InvalidConfigs.py | 151 + .../files/templates/basic_flex_rtp_v1.1.py | 522 + ...s_TooTallLabware_Override_south_west].json | 1500 + ...es_TooTallLabware_Override_north_row].json | 1500 + ...][Flex_S_v2_20_96_None_ROW_HappyPath].json | 6224 +++ ...c2386b92][Flex_S_v2_20_96_AllCorners].json | 33713 ++++++++++++++++ ...a9c68b][Flex_S_v2_20_P8X1000_P50_LLD].json | 6191 +++ ...errides_TooTallLabware_Override_west].json | 1500 + ...ooTallLabware_Override_mix_collision].json | 2746 ++ ...ide_distribute_destination_collision].json | 3930 ++ ...e_Override_transfer_source_collision].json | 3897 ++ ..._TooTallLabware_Override_east_column].json | 1500 + ..._S_v2_20_96_None_SINGLE_4Corners50ul].json | 3036 ++ ...20_96_None_SINGLE_HappyPathNorthSide].json | 9380 +++++ ...allLabware_Override_bottom_left_edge].json | 1268 + ...allLabware_Override_bottom_left_edge].json | 1268 + ...es_TooTallLabware_Override_south_row].json | 1500 + ...ARTIAL_COLUMN_HappyPathMixedTipRacks].json | 19487 +++++++++ ...Override_eight_partial_column_no_end].json | 1260 + ...ide_eight_partial_column_bottom_left].json | 1296 + ...ooTallLabware_Override_c3_right_edge].json | 1268 + ...Override_eight_partial_column_no_end].json | 2414 ++ ...Override_ninety_six_partial_column_2].json | 1260 + ...Override_ninety_six_partial_column_1].json | 1260 + ...s_TooTallLabware_Override_south_east].json | 1500 + ...errides_TooTallLabware_Override_east].json | 1500 + ...lex_S_v2_20_96_None_COLUMN_HappyPath].json | 6224 +++ ...de_consolidate_destination_collision].json | 3963 ++ ...2_20_8_None_PARTIAL_COLUMN_HappyPath].json | 5041 +++ ...lidConfigs_Override_return_tip_error].json | 4939 +++ ..._TooTallLabware_Override_west_column].json | 1500 + ...rrides_TooTallLabware_Override_south].json | 1500 + ...figs_Override_drop_tip_with_location].json | 3785 ++ ...de_eight_partial_column_bottom_right].json | 2480 ++ ...s_TooTallLabware_Override_north_west].json | 1500 + ...20_96_None_SINGLE_HappyPathSouthSide].json | 9380 +++++ ...ide_eight_partial_column_bottom_left].json | 2450 ++ ...lidConfigs_Override_return_tip_error].json | 3785 ++ ...figs_Override_drop_tip_with_location].json | 4939 +++ ...rride_transfer_destination_collision].json | 3930 ++ ...rrides_TooTallLabware_Override_north].json | 1500 + ...llLabware_Override_bottom_right_edge].json | 1268 + ...Flex_S_v2_20_8_None_SINGLE_HappyPath].json | 7137 ++++ ...de_eight_partial_column_bottom_right].json | 1326 + ...hot[d2c818bf00][Flex_S_v2_20_P50_LPD].json | 5125 +++ ...[OT2_S_v2_20_8_None_SINGLE_HappyPath].json | 8190 ++++ ...Override_ninety_six_partial_column_3].json | 1260 + ...des_TooTallLabware_Override_top_edge].json | 1268 + ...Flex_S_v2_20_96_None_Column3_SINGLE_].json | 26859 ++++++++++++ ...verride_consolidate_source_collision].json | 3897 ++ ...Override_distribute_source_collision].json | 3897 ++ 86 files changed, 232337 insertions(+) create mode 100644 analyses-snapshot-testing/citools/Dockerfile.server create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_1000M_Simple.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_PARTIAL_COLUMN_HappyPath.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_SINGLE_HappyPath.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_SINGLE_TubeRackCollision.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_PARTIAL_COLUMN_Overhang.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_TipsDontMatchWells.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_AllCorners.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_LLD.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_COLUMN_HappyPath.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_Column3_SINGLE_.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_ROW_HappyPath.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_4Corners50ul.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathNorthSide.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathSouthSide.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_TubeRackCollision.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_Reservoir.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_LPD.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_LPD_wet_tip_scenarios.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_touch_tip_directly.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P8X1000_P50_LLD.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_None_Overrides_TooTallLabware.py create mode 100644 analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs.py create mode 100644 analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_PARTIAL_COLUMN_HappyPathMixedTipRacks.py create mode 100644 analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_SINGLE_HappyPath.py create mode 100644 analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_SINGLE_TubeRackCollision.py create mode 100644 analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P20M_PARTIAL_COLUMN_Overhang.py create mode 100644 analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P20M_Simple.py create mode 100644 analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P300M_PARTIAL_COLUMN_Overhang.py create mode 100644 analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P300M_Simple.py create mode 100644 analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P50_touch_tip.py create mode 100644 analyses-snapshot-testing/files/protocols/OT2_X_v2_20_8_Overrides_InvalidConfigs.py create mode 100644 analyses-snapshot-testing/files/templates/basic_flex_rtp_v1.1.py create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[09676b9f7e][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_south_west].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[0b42cfc151][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_north_row].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[109b7ad1f0][Flex_S_v2_20_96_None_ROW_HappyPath].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[10c2386b92][Flex_S_v2_20_96_AllCorners].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[13e5a9c68b][Flex_S_v2_20_P8X1000_P50_LLD].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[196ec488f7][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_west].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[255d014c70][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_mix_collision].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[29e143f047][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_distribute_destination_collision].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[29eec5a85a][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_transfer_source_collision].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[2cc8efae68][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_east_column].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[30c05024e8][Flex_S_v2_20_96_None_SINGLE_4Corners50ul].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[31d1aec819][Flex_S_v2_20_96_None_SINGLE_HappyPathNorthSide].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[376765183a0][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_bottom_left_edge].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[376765183a1][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_bottom_left_edge].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[39191e3573][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_south_row].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[4b9e0f93d9][OT2_S_v2_20_8_None_PARTIAL_COLUMN_HappyPathMixedTipRacks].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[4ea9d66206][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_eight_partial_column_no_end].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[53db9bf516][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_eight_partial_column_bottom_left].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[549cc904ac][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_c3_right_edge].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[54b0b509cd][OT2_X_v2_20_8_Overrides_InvalidConfigs_Override_eight_partial_column_no_end].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[5edb9b4de3][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_2].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[6122c72996][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_1].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[675d2c2562][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_south_east].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[68614da0b3][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_east].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[6c20d6c570][Flex_S_v2_20_96_None_COLUMN_HappyPath].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[6e2f6f10c5][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_consolidate_destination_collision].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[733c9cdf62][Flex_S_v2_20_8_None_PARTIAL_COLUMN_HappyPath].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[7583faec7c][OT2_X_v2_20_8_Overrides_InvalidConfigs_Override_return_tip_error].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[7e7a90041b][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_west_column].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[8a663305c4][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_south].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[8b07e799f6][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_drop_tip_with_location].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[93b724671e][OT2_X_v2_20_8_Overrides_InvalidConfigs_Override_eight_partial_column_bottom_right].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[a0b755a1a1][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_north_west].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[a8528e52b4][Flex_S_v2_20_96_None_SINGLE_HappyPathSouthSide].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[acefe91275][OT2_X_v2_20_8_Overrides_InvalidConfigs_Override_eight_partial_column_bottom_left].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[afd5d372a9][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_return_tip_error].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[b79134ab8a][OT2_X_v2_20_8_Overrides_InvalidConfigs_Override_drop_tip_with_location].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[bc049301c1][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_transfer_destination_collision].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[c0435f08da][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_north].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[c343dfb5a0][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_bottom_right_edge].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[cc26c104b4][Flex_S_v2_20_8_None_SINGLE_HappyPath].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[d0154b1493][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_eight_partial_column_bottom_right].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[d2c818bf00][Flex_S_v2_20_P50_LPD].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[d979799443][OT2_S_v2_20_8_None_SINGLE_HappyPath].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[db1fae41ec][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_3].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[e84e23a4ea][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_top_edge].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[e8f451df45][Flex_S_v2_20_96_None_Column3_SINGLE_].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[ed26635ff7][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_consolidate_source_collision].json create mode 100644 analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[f86713b4d4][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_distribute_source_collision].json diff --git a/analyses-snapshot-testing/Makefile b/analyses-snapshot-testing/Makefile index e0aa23a8415..fff00bbe3c1 100644 --- a/analyses-snapshot-testing/Makefile +++ b/analyses-snapshot-testing/Makefile @@ -89,3 +89,26 @@ build-opentrons-analysis: .PHONY: generate-protocols generate-protocols: python -m pipenv run python -m automation.data.protocol_registry + + +OPENTRONS_VERSION ?= edge +export OPENTRONS_VERSION + +.PHONY: build-rs +build-rs: + @echo "Building docker image for opentrons-robot-server:$(OPENTRONS_VERSION)" + @echo "Cache is always busted to ensure latest version of the code is used" + @echo "If you want to build a different version, run 'make build-rs OPENTRONS_VERSION=chore_release-8.0.0'" + docker build --build-arg OPENTRONS_VERSION=$(OPENTRONS_VERSION) --build-arg CACHEBUST=$(CACHEBUST) -t opentrons-robot-server:$(OPENTRONS_VERSION) -f citools/Dockerfile.server . + +.PHONY: run-flex +run-flex: + @echo "Running opentrons-robot-server:$(OPENTRONS_VERSION)" + @echo "If you want to run a different version, run 'make run-flex OPENTRONS_VERSION=chore_release-8.0.0'" + docker run -p 31950:31950 --env-file ../robot-server/dev-flex.env opentrons-robot-server:$(OPENTRONS_VERSION) + +.PHONY: run-ot2 +run-ot2: + @echo "Running opentrons-robot-server:$(OPENTRONS_VERSION)" + @echo "If you want to run a different version, run 'make run-ot2 OPENTRONS_VERSION=chore_release-8.0.0'" + docker run -p 31950:31950 --env-file ../robot-server/dev.env opentrons-robot-server:$(OPENTRONS_VERSION) diff --git a/analyses-snapshot-testing/README.md b/analyses-snapshot-testing/README.md index 1ed330efefe..51a8e194ca1 100644 --- a/analyses-snapshot-testing/README.md +++ b/analyses-snapshot-testing/README.md @@ -14,6 +14,12 @@ - In CI this is the `SNAPSHOT_REF`. This is the branch or tag of the test code/snapshots that analyses generated will be compared to. - The `ANALYSIS_REF` is the branch or tag that you want analyses generated from. +## Build the opentrons-analysis image + +> This ALWAYS gets the remote code pushed to Opentrons/opentrons for the specified ANALYSIS_REF + +`make build-opentrons-analysis ANALYSIS_REF=chore_release-8.0.0` + ## Running the tests locally - Compare the current branch snapshots to analyses generated from the edge branch @@ -38,3 +44,17 @@ - `make snapshot-test PROTOCOL_NAMES=Flex_S_v2_19_Illumina_DNA_PCR_Free OVERRIDE_PROTOCOL_NAMES=none` - `make snapshot-test PROTOCOL_NAMES=none OVERRIDE_PROTOCOL_NAMES=Flex_X_v2_18_NO_PIPETTES_Overrides_BadTypesInRTP` - `make snapshot-test PROTOCOL_NAMES="Flex_S_v2_19_Illumina_DNA_PCR_Free,OT2_S_v2_18_P300M_P20S_HS_TC_TM_SmokeTestV3" OVERRIDE_PROTOCOL_NAMES=none` + +## Running a Flex just like `make -C robot-server dev-flex` + +> This ALWAYS gets the remote code pushed to Opentrons/opentrons for the specified OPENTRONS_VERSION + +```shell +cd analyses-snapshot-testing \ +&& make build-rs OPENTRONS_VERSION=chore_release-8.0.0 \ +&& make run-rs OPENTRONS_VERSION=chore_release-8.0.0` +``` + +### Default OPENTRONS_VERSION=edge in the Makefile so you can omit it if you want latest edge + +`cd analyses-snapshot-testing && make build-rs && make run-rs` diff --git a/analyses-snapshot-testing/automation/data/protocols.py b/analyses-snapshot-testing/automation/data/protocols.py index 580c9183d9d..ada74a736e0 100644 --- a/analyses-snapshot-testing/automation/data/protocols.py +++ b/analyses-snapshot-testing/automation/data/protocols.py @@ -641,6 +641,74 @@ class Protocols: robot="Flex", ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_PARTIAL_COLUMN_HappyPath.py + Flex_S_v2_20_8_None_PARTIAL_COLUMN_HappyPath: Protocol = Protocol( + file_stem="Flex_S_v2_20_8_None_PARTIAL_COLUMN_HappyPath", + file_extension="py", + robot="Flex", + ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_SINGLE_HappyPath.py + Flex_S_v2_20_8_None_SINGLE_HappyPath: Protocol = Protocol( + file_stem="Flex_S_v2_20_8_None_SINGLE_HappyPath", + file_extension="py", + robot="Flex", + ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_AllCorners.py + Flex_S_v2_20_96_AllCorners: Protocol = Protocol( + file_stem="Flex_S_v2_20_96_AllCorners", + file_extension="py", + robot="Flex", + ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_COLUMN_HappyPath.py + Flex_S_v2_20_96_None_COLUMN_HappyPath: Protocol = Protocol( + file_stem="Flex_S_v2_20_96_None_COLUMN_HappyPath", + file_extension="py", + robot="Flex", + ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_Column3_SINGLE_.py + Flex_S_v2_20_96_None_Column3_SINGLE_: Protocol = Protocol( + file_stem="Flex_S_v2_20_96_None_Column3_SINGLE_", + file_extension="py", + robot="Flex", + ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_ROW_HappyPath.py + Flex_S_v2_20_96_None_ROW_HappyPath: Protocol = Protocol( + file_stem="Flex_S_v2_20_96_None_ROW_HappyPath", + file_extension="py", + robot="Flex", + ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_4Corners50ul.py + Flex_S_v2_20_96_None_SINGLE_4Corners50ul: Protocol = Protocol( + file_stem="Flex_S_v2_20_96_None_SINGLE_4Corners50ul", + file_extension="py", + robot="Flex", + ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathNorthSide.py + Flex_S_v2_20_96_None_SINGLE_HappyPathNorthSide: Protocol = Protocol( + file_stem="Flex_S_v2_20_96_None_SINGLE_HappyPathNorthSide", + file_extension="py", + robot="Flex", + ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathSouthSide.py + Flex_S_v2_20_96_None_SINGLE_HappyPathSouthSide: Protocol = Protocol( + file_stem="Flex_S_v2_20_96_None_SINGLE_HappyPathSouthSide", + file_extension="py", + robot="Flex", + ) + + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P8X1000_P50_LLD.py + Flex_S_v2_20_P8X1000_P50_LLD: Protocol = Protocol( + file_stem="Flex_S_v2_20_P8X1000_P50_LLD", + file_extension="py", + robot="Flex", + ) + # analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_LPD.py + Flex_S_v2_20_P50_LPD: Protocol = Protocol( + file_stem="Flex_S_v2_20_P50_LPD", + file_extension="py", + robot="Flex", + ) + OT2_X_v2_18_None_None_duplicateRTPVariableName: Protocol = Protocol( file_stem="OT2_X_v2_18_None_None_duplicateRTPVariableName", file_extension="py", @@ -677,6 +745,19 @@ class Protocols: robot="OT2", ) + # analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_PARTIAL_COLUMN_HappyPathMixedTipRacks.py + OT2_S_v2_20_8_None_PARTIAL_COLUMN_HappyPathMixedTipRacks: Protocol = Protocol( + file_stem="OT2_S_v2_20_8_None_PARTIAL_COLUMN_HappyPathMixedTipRacks", + file_extension="py", + robot="OT2", + ) + # analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_SINGLE_HappyPath.py + OT2_S_v2_20_8_None_SINGLE_HappyPath: Protocol = Protocol( + file_stem="OT2_S_v2_20_8_None_SINGLE_HappyPath", + file_extension="py", + robot="OT2", + ) + ########################################################################################################## # Begin Protocol Library Protocols ####################################################################### ########################################################################################################## diff --git a/analyses-snapshot-testing/automation/data/protocols_with_overrides.py b/analyses-snapshot-testing/automation/data/protocols_with_overrides.py index ff4a900e58d..5018a848c31 100644 --- a/analyses-snapshot-testing/automation/data/protocols_with_overrides.py +++ b/analyses-snapshot-testing/automation/data/protocols_with_overrides.py @@ -38,3 +38,72 @@ class ProtocolsWithOverrides: override_variable_name="type_to_test", overrides=["str_default_no_matching_choices", "float_default_no_matching_choices", "int_default_no_matching_choices"], ) + + # analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs.py + + Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs: ProtocolWithOverrides = ProtocolWithOverrides( + file_stem="Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs", + file_extension="py", + robot="Flex", + override_variable_name="key", + overrides=[ + "ninety_six_partial_column_1", + "ninety_six_partial_column_2", + "ninety_six_partial_column_3", + "eight_partial_column_bottom_left", + "eight_partial_column_bottom_right", + "eight_partial_column_no_end", + "return_tip_error", + "drop_tip_with_location", + ], + ) + + # analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_None_Overrides_TooTallLabware.py + + Flex_X_v2_20_96_None_Overrides_TooTallLabware: ProtocolWithOverrides = ProtocolWithOverrides( + file_stem="Flex_X_v2_20_96_None_Overrides_TooTallLabware", + file_extension="py", + robot="Flex", + override_variable_name="key", + overrides=[ + "transfer_source_collision", + "transfer_destination_collision", + "c3_right_edge", + "north", + "north_west", + "west", + "south_west", + "south", + "south_east", + "east", + "east_column", + "west_column", + "north_row", + "south_row", + "top_edge", + "bottom_left_edge", + "bottom_left_edge", + "bottom_right_edge", + "mix_collision", + "consolidate_source_collision", + "consolidate_destination_collision", + "distribute_source_collision", + "distribute_destination_collision", + ], + ) + + # analyses-snapshot-testing/files/protocols/OT2_X_v2_20_8_Overrides_InvalidConfigs.py + + OT2_X_v2_20_8_Overrides_InvalidConfigs: ProtocolWithOverrides = ProtocolWithOverrides( + file_stem="OT2_X_v2_20_8_Overrides_InvalidConfigs", + file_extension="py", + robot="Flex", + override_variable_name="key", + overrides=[ + "eight_partial_column_bottom_left", + "eight_partial_column_bottom_right", + "eight_partial_column_no_end", + "return_tip_error", + "drop_tip_with_location", + ], + ) diff --git a/analyses-snapshot-testing/citools/Dockerfile.server b/analyses-snapshot-testing/citools/Dockerfile.server new file mode 100644 index 00000000000..6d4d9edcda3 --- /dev/null +++ b/analyses-snapshot-testing/citools/Dockerfile.server @@ -0,0 +1,31 @@ +# Use Python 3.10 as the base image +FROM python:3.10-slim-bullseye + +# Update packages and install dependencies +RUN apt-get update && \ + apt-get upgrade -y && \ + apt-get install -y git libsystemd-dev build-essential pkg-config network-manager + +# Define build arguments +ARG OPENTRONS_VERSION=edge + +# Set the working directory +WORKDIR /opentrons + +# Clone the Opentrons repository +ARG CACHEBUST=1 +RUN git clone --branch $OPENTRONS_VERSION --depth 1 https://github.com/Opentrons/opentrons . + +# Install dependencies +RUN make setup-py -j + +WORKDIR /opentrons/robot-server + +# Set the port via environment variable +ENV PORT=31950 + +# Expose the port +EXPOSE ${PORT} + +# Default command +CMD ["sh", "-c", "python -m pipenv run uvicorn robot_server.app:app --host 0.0.0.0 --port ${PORT} --ws wsproto --lifespan on"] diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_1000M_Simple.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_1000M_Simple.py new file mode 100644 index 00000000000..36fe24def92 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_1000M_Simple.py @@ -0,0 +1,93 @@ +from opentrons.protocol_api import PARTIAL_COLUMN + +metadata = { + "protocolName": "flex_8channel_1000 Simple", + "description": "A protocol that demonstrates safe actions with flex_8channel_1000", + "author": "Josh McVey", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="Tip Rack", + location="A1", + ) + + pipette = protocol.load_instrument(instrument_name="flex_8channel_1000", mount="left", tip_racks=[tip_rack]) + + source_labware_C2 = protocol.load_labware( + load_name="nest_1_reservoir_290ml", + label="Source Reservoir", + location="C2", + ) + + destination_labware_D2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="PCR Plate", + location="2", + ) + + volume = 100 + pipette.pick_up_tip() + comment_tip_rack_status(protocol, tip_rack) + pipette.aspirate(volume=volume, location=destination_labware_D2["H1"]) + pipette.dispense(volume=volume, location=destination_labware_D2["H2"]) + for i in range(1, 13): + protocol.comment(f"Touching tip to {destination_labware_D2[f'H{i}']}") + pipette.touch_tip(location=destination_labware_D2[f"H{i}"]) + + pipette.blow_out(location=destination_labware_D2["H1"]) + pipette.mix(repetitions=3, volume=volume, location=destination_labware_D2["H1"]) + pipette.drop_tip() + + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + column1 = [destination_labware_D2[f"{row}1"] for row in range_A_to_H] + column2 = [destination_labware_D2[f"{row}2"] for row in range_A_to_H] + column3 = [destination_labware_D2[f"{row}3"] for row in range_A_to_H] + column4 = [destination_labware_D2[f"{row}4"] for row in range_A_to_H] + column5 = [destination_labware_D2[f"{row}5"] for row in range_A_to_H] + column6 = [destination_labware_D2[f"{row}6"] for row in range_A_to_H] + column7 = [destination_labware_D2[f"{row}7"] for row in range_A_to_H] + + protocol.comment(f"Transferring {volume}uL from column 1 to column 2") + pipette.transfer(volume=volume, source=column1, dest=column2) + comment_tip_rack_status(protocol, tip_rack) + + volume = 50 + protocol.comment(f"Distribute {volume}uL from column 2 to column 3 and 4") + pipette.distribute(volume=volume, source=column2, dest=column3 + column4) + comment_tip_rack_status(protocol, tip_rack) + protocol.comment(f"Consolidate {volume}uL from column 5 and 6 to column 7") + pipette.consolidate(volume=volume, source=column5 + column6, dest=column7) + comment_tip_rack_status(protocol, tip_rack) diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_PARTIAL_COLUMN_HappyPath.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_PARTIAL_COLUMN_HappyPath.py new file mode 100644 index 00000000000..cde6baf358f --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_PARTIAL_COLUMN_HappyPath.py @@ -0,0 +1,109 @@ +from opentrons.protocol_api import PARTIAL_COLUMN + +metadata = { + "protocolName": "8 Channel PARTIAL_COLUMN Happy Path A1 or H1", + "description": "Tip Rack South Clearance for the 8 Channel pipette and a SINGLE partial tip configuration.", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + thermocycler = protocol.load_module("thermocycler module gen2") + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_50ul", + label="Partial Tip Rack", + location="B3", + ) + + pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="right") + # mount on the right and you will get an error. + + pipette.configure_nozzle_layout( + style=PARTIAL_COLUMN, + start="H1", + end="B1", # 2 Tips + tip_racks=[partial_tip_rack], + ) + + source_labware_B2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="B2 Source Labware", + location="B2", + ) + + destination_labware_C2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="C2 Destination Labware", + location="C2", + ) + + volume = 10 # Default volume for actions that require it + + # ############################# + # # Pipette do work + # # leading nozzle + # # index in based on eh number of tips in my current configuration + # pipette.transfer(volume, source_labware_B2["B6"], destination_labware_C2["A6"]) + + # pipette.pick_up_tip() + # pipette.touch_tip(source_labware_B2["B1"]) + # pipette.drop_tip() + # pipette.pick_up_tip() + # pipette.home() + # pipette.drop_tip() + + # pipette.pick_up_tip() + # well = source_labware_B2["D1"] + # # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + # pipette.move_to(well.bottom(z=2)) + # pipette.mix(10, 10) + # pipette.move_to(well.top(z=5)) + # pipette.blow_out() + # pipette.prepare_to_aspirate() + # pipette.move_to(well.bottom(z=2)) + # pipette.aspirate(10, well.bottom(z=2)) + # pipette.dispense(10) + # pipette.drop_tip() + + # ############################ + # # Change the pipette configuration + + # pipette.configure_nozzle_layout( + # style=PARTIAL_COLUMN, + # start="H1", + # end="B1", # 7 Tips + # tip_racks=[partial_tip_rack], + # ) + + ############################# + # Pipette do work + + pipette.transfer(volume, source_labware_B2["A6"], destination_labware_C2["A6"]) + + pipette.pick_up_tip() + pipette.touch_tip(source_labware_B2["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware_B2["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_SINGLE_HappyPath.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_SINGLE_HappyPath.py new file mode 100644 index 00000000000..95493eb0ab3 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_SINGLE_HappyPath.py @@ -0,0 +1,131 @@ +from opentrons.protocol_api import SINGLE + +metadata = { + "protocolName": "8 Channel SINGLE Happy Path A1 or H1", + "description": "Tip Rack South Clearance for the 8 Channel pipette and a SINGLE partial tip configuration.", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_50ul", + label="Partial Tip Rack", + location="B3", + ) + + thermocycler = protocol.load_module("thermocycler module gen2") + + pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="right") + # mount on the right and you will get an error. + + # On the 8-channel SINGLE + # start="A1" Means the South nozzle will pickup from the NW corner of the tip rack + # start="H1" Means the North nozzle will pickup from the SW corner of the tip rack + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", # Which tip to start with + tip_racks=[partial_tip_rack], + ) + + source_labware_B2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="B2 Source Labware", + location="B2", + ) + + destination_labware_C2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="C2 Destination Labware", + location="C2", + ) + + volume = 10 # Default volume for actions that require it + + ############################# + # Pipette do work + pipette.consolidate( + [volume, volume], + [source_labware_B2["A3"], source_labware_B2["A4"]], + destination_labware_C2["A3"], + ) + + pipette.transfer(volume, source_labware_B2["A6"], destination_labware_C2["A6"]) + + pipette.distribute( + 5, + source_labware_B2["A7"], + [destination_labware_C2["A7"], destination_labware_C2["A8"]], + ) + + pipette.pick_up_tip() + pipette.touch_tip(source_labware_B2["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware_B2["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() + + ############################ + # Change the pipette configuration + + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", # Which tip to start with + tip_racks=[partial_tip_rack], + ) + + ############################# + # Pipette do work + pipette.consolidate( + [volume, volume], + [source_labware_B2["A3"], source_labware_B2["A4"]], + destination_labware_C2["A3"], + ) + + pipette.transfer(volume, source_labware_B2["A6"], destination_labware_C2["A6"]) + + pipette.distribute( + 5, + source_labware_B2["A7"], + [destination_labware_C2["A7"], destination_labware_C2["A8"]], + ) + + pipette.pick_up_tip() + pipette.touch_tip(source_labware_B2["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware_B2["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_SINGLE_TubeRackCollision.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_SINGLE_TubeRackCollision.py new file mode 100644 index 00000000000..4f7a21d58bb --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_None_SINGLE_TubeRackCollision.py @@ -0,0 +1,65 @@ +from opentrons.protocol_api import SINGLE + +metadata = { + "protocolName": "8Channel SINGLE Pickup tuberack collision", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_50ul", + label="Partial Tip Rack", + location="D2", + ) + + tube_rack = protocol.load_labware( + load_name="opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical", + label="Tube Rack", + location="B2", + ) + + pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="left") + + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", + tip_racks=[partial_tip_rack], + ) + + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + # can the flex_8channel_50 with 50μl tips really reach the bottom of the tube? + pipette.aspirate(30, tube_rack["A1"].bottom()) + pipette.dispense(30, tube_rack["A2"].bottom()) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_PARTIAL_COLUMN_Overhang.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_PARTIAL_COLUMN_Overhang.py new file mode 100644 index 00000000000..b1bcf5ed77b --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_PARTIAL_COLUMN_Overhang.py @@ -0,0 +1,150 @@ +from opentrons.protocol_api import PARTIAL_COLUMN + +metadata = { + "protocolName": "8 Channel PARTIAL_COLUMN Overhang", + "description": "A protocol that demonstrates overhang into a slot with a partial column configuration.", + "author": "Josh McVey", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + thermocycler = protocol.load_module("thermocycler module gen2") + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_50ul", + label="Partial Tip Rack", + location="B3", + ) + + pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="right") + + pipette.configure_nozzle_layout( + style=PARTIAL_COLUMN, + start="H1", + end="D1", # 5 Tips + tip_racks=[partial_tip_rack], + ) + + source_labware_B2 = protocol.load_labware( + load_name="nest_1_reservoir_290ml", + label="B2 Source Reservoir", + location="B2", + ) + + destination_labware_C2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="C2 Destination Labware", + location="C2", + ) + + volume = 10 # Default volume for actions that require it + + # Known issue in 8.0.0 + # if you target A1 of the labware in C2 + # nozzle H1 - the front-most nozzle will go to A1 + # this means that tips will be out of the slot + # they will over hang into the B2 + # no error is raised + # but a collision will occur with the labware in slot B2 + # the overhanging tips will collide with the labware in B2 + + # If we ever do detect and error for this + # Take all these examples into a negative Overrides test + + volume = 20 + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + # bad - 4 tips will overhang into B2 + pipette.aspirate(volume=5, location=destination_labware_C2["A1"]) + # bad - 3 tips will overhang into B2 + pipette.aspirate(volume=5, location=destination_labware_C2["B1"]) + # bad - 2 tips will overhang into B2 + pipette.aspirate(volume=5, location=destination_labware_C2["C1"]) + # bad - 1 tip will overhang into B2 + pipette.aspirate(volume=5, location=destination_labware_C2["D1"]) + # this is safe + pipette.aspirate(volume=5, location=destination_labware_C2["E1"]) + + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_B2["A1"]) + # bad - bad - 4 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_C2["A1"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_B2["A1"]) + # bad - bad - 3 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_C2["B2"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_B2["A1"]) + # bad - bad - 2 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_C2["C3"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_B2["A1"]) + # bad - bad - 1 tip will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_C2["D4"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_B2["A1"]) + # this is safe - 0 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_C2["E5"]) + # bad - has overhang into B2 - 4 tips + pipette.touch_tip(location=destination_labware_C2["A1"]) + # bad - has overhang into B2 - 4 tips + pipette.blow_out(location=destination_labware_C2["A1"]) + # bad - has overhang into B2 - 4 tips + pipette.mix(repetitions=3, volume=volume, location=destination_labware_C2["A2"]) + # bad - aspirate and dispense have overhang into B2 + pipette.drop_tip() + pipette.transfer(volume=volume, source=destination_labware_C2["A1"], dest=destination_labware_C2["A2"]) + # bad - aspirate has 3 tip overhang but not dispense + pipette.transfer(volume=volume, source=destination_labware_C2["B1"], dest=destination_labware_C2["E1"]) + # bad - aspirate is safe but dispense has 2 tip overhang + pipette.transfer(volume=volume, source=destination_labware_C2["E1"], dest=destination_labware_C2["C3"]) + # bad - source and destinations have overhang + pipette.distribute(volume=20, source=destination_labware_C2["D2"], dest=[destination_labware_C2["D3"], destination_labware_C2["D4"]]) + # bad - source has overhang but destinations are safe + pipette.distribute(volume=20, source=destination_labware_C2["D2"], dest=[destination_labware_C2["E3"], destination_labware_C2["E4"]]) + # bad - source has no overhang but 1 destination does + pipette.distribute(volume=20, source=destination_labware_C2["E6"], dest=[destination_labware_C2["A7"], destination_labware_C2["E7"]]) + # bad - source has no overhang but 2 destinations do + pipette.distribute(volume=20, source=destination_labware_C2["E7"], dest=[destination_labware_C2["A8"], destination_labware_C2["A9"]]) + # bad - all sources and destination have overhang + pipette.consolidate(volume=5, source=[destination_labware_C2["A9"], destination_labware_C2["A10"]], dest=destination_labware_C2["A11"]) + # bad - 1 source has overhang but destination is safe + pipette.consolidate(volume=5, source=[destination_labware_C2["A9"], destination_labware_C2["E10"]], dest=destination_labware_C2["E11"]) + # bad - sources are safe but destination has overhang + pipette.consolidate(volume=5, source=[destination_labware_C2["E9"], destination_labware_C2["E10"]], dest=destination_labware_C2["A12"]) diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_TipsDontMatchWells.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_TipsDontMatchWells.py new file mode 100644 index 00000000000..a6ecaf02340 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_8_TipsDontMatchWells.py @@ -0,0 +1,87 @@ +from opentrons.protocol_api import SINGLE, ALL + +metadata = { + "protocolName": "8channel 50 into a 48 well plate", + "description": "the nozzles on the pipette do not match the target labware wells", + "author": "Josh McVey", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_50ul", + label="Partial Tip Rack", + location="B3", + ) + + # corning_48_wellplate_1.6ml_flat + corning_48 = protocol.load_labware( + load_name="corning_48_wellplate_1.6ml_flat", + label="Corning 48 Wellplate", + location="D2", + ) + + pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="right") + + pipette.configure_nozzle_layout( + style=ALL, + start="H1", + end="B1", # Test that end is ignored when style is ALL and on 8.0.0-alpha.6 it is ✅ + tip_racks=[partial_tip_rack], + ) + + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + volume = 20 + + ###### Purpose statement + ### This protocol is made to detect if analysis output ever changes + ### When we try and pipette to wells that do not match the geometry of the nozzles on the pipette + ###### + + # when using 8 channel location is the front-most well in the column of the target labware + pipette.aspirate(volume=volume, location=corning_48["F1"]) + pipette.dispense(volume=volume, location=corning_48["F1"]) + pipette.touch_tip(location=corning_48["F1"]) + pipette.blow_out(location=corning_48["F1"]) + pipette.mix(repetitions=3, volume=volume, location=corning_48["F1"]) + # next line has this error: Invalid source for multichannel transfer: [F1 of Corning 48 Wellplate on slot D2] + # pipette.transfer(volume=volume, source=corning_48["F1"], dest=corning_48["F2"]) + # next line has this error: Invalid source for multichannel transfer: [F2 of Corning 48 Wellplate on slot D2] + # pipette.distribute(volume=20, source=corning_48["F2"], dest=[corning_48["F3"], corning_48["F4"]]) + # next line has this error: + # Invalid source for multichannel transfer: [F6 of Corning 48 Wellplate on slot D2, F7 of Corning 48 Wellplate on slot D2] + # pipette.consolidate(volume=20, source=[corning_48["F6"], corning_48["F7"]], dest=corning_48["F8"]) + + pipette.drop_tip() # so trash bin shows up in the deck map diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_AllCorners.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_AllCorners.py new file mode 100644 index 00000000000..01fc0b45b9b --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_AllCorners.py @@ -0,0 +1,296 @@ +from opentrons import protocol_api +from opentrons.protocol_api import COLUMN, SINGLE, ROW + +requirements = {"robotType": "Flex", "apiLevel": "2.20"} + + +def run(protocol: protocol_api.ProtocolContext): + pipette = protocol.load_instrument(instrument_name="flex_96channel_1000") + trash = protocol.load_trash_bin("B3") + t1 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="A1 Corner Tiprack 1", + location="B1", + ) + t2 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="D1 Corner Tiprack", + location="C1", + ) + t3 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="A3 Corner Tiprack 1", + location="B2", + ) + t4 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="D3 Corner Tiprack", + location="C2", + ) + t5 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="A3 Corner Tiprack 2", + location="A2", + ) + t6 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="A1 Corner Tiprack 2", + location="D4", + ) + + ### SETUP TIPRACK FUNCTIONS + # These functions serve the purpose of removing tips from a tiprack before it is moved to a corner slot + # This is done to ensure the tiprack is in such a state that it will trigger zero deck extent conflicts + + # Setup T2 + def t2_setup() -> None: + pipette.configure_nozzle_layout( + style=ROW, + start="A1", + tip_racks=[t2], + ) + for i in range(3): + pipette.pick_up_tip() + pipette.drop_tip() + + # Setup T3 + def t3_setup() -> None: + pipette.configure_nozzle_layout(style=COLUMN, start="A1", tip_racks=[t3]) + for i in range(2): + pipette.pick_up_tip() + pipette.drop_tip() + + # Setup T4 + def t4_setup() -> None: + pipette.configure_nozzle_layout( + style=ROW, + start="A1", + tip_racks=[t4], + ) + for i in range(3): + pipette.pick_up_tip() + pipette.drop_tip() + + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", + tip_racks=[t4], + ) + for i in range(10): + pipette.pick_up_tip() + pipette.drop_tip() + + # Setup T5 + def t5_setup() -> None: + pipette.configure_nozzle_layout( + style=ROW, + start="H1", + tip_racks=[t5], + ) + for i in range(7): + pipette.pick_up_tip() + pipette.drop_tip() + + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", + tip_racks=[t5], + ) + for i in range(2): + pipette.pick_up_tip() + pipette.drop_tip() + + # Setup T6 + def t6_setup() -> None: + pipette.configure_nozzle_layout( + style=ROW, + start="H1", + tip_racks=[t6], + ) + for i in range(7): + pipette.pick_up_tip() + pipette.drop_tip() + + ### PICKUP TIP FUNCTIONS + # These functions perform pickup tip behavior for a given tiprack + + # Pickup T2 + def t1_pickup() -> None: + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", + tip_racks=[t1], + ) + for i in range(48): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="A12", + tip_racks=[t1], + ) + for i in range(48): + pipette.pick_up_tip() + pipette.drop_tip() + + # Pickup T2 + def t2_pickup() -> None: + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", + tip_racks=[t2], + ) + for i in range(15): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="A12", + tip_racks=[t2], + ) + for i in range(15): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", + tip_racks=[t2], + ) + for i in range(15): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="H12", + tip_racks=[t2], + ) + for i in range(15): + pipette.pick_up_tip() + pipette.drop_tip() + + # Pickup T3 + def t3_pickup() -> None: + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", + tip_racks=[t3], + ) + for i in range(40): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="A12", + tip_racks=[t3], + ) + for i in range(40): + pipette.pick_up_tip() + pipette.drop_tip() + + # Pickup T4 + def t4_pickup() -> None: + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", + tip_racks=[t4], + ) + for i in range(10): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="A12", + tip_racks=[t4], + ) + for i in range(10): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", + tip_racks=[t4], + ) + for i in range(15): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="H12", + tip_racks=[t4], + ) + for i in range(15): + pipette.pick_up_tip() + pipette.drop_tip() + + # Pickup T5 + def t5_pickup() -> None: + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", + tip_racks=[t5], + ) + for i in range(5): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="H12", + tip_racks=[t5], + ) + for i in range(5): + pipette.pick_up_tip() + pipette.drop_tip() + + # Pickup T6 + def t6_pickup() -> None: + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", + tip_racks=[t6], + ) + for i in range(6): + pipette.pick_up_tip() + pipette.drop_tip() + pipette.configure_nozzle_layout( + style=SINGLE, + start="H12", + tip_racks=[t6], + ) + for i in range(6): + pipette.pick_up_tip() + pipette.drop_tip() + + ### Protocol Actions + # Perform Setup of first set of tipracks + protocol.move_labware(t1, "A1", True) + t2_setup() + protocol.move_labware(t2, "D1", True) + t3_setup() + protocol.move_labware(t3, "A3", True) + t4_setup() + protocol.move_labware(t4, "D3", True) + + # Clear out T2 first to make room for T5 + t2_pickup() + protocol.move_labware(t5, "B2", True) + t5_setup() + protocol.move_labware(t5, "C1", True) + + # Clear out T1, T3, and T4 + t1_pickup() + t3_pickup() + protocol.move_labware(t6, "A4", True) + t4_pickup() + protocol.move_labware(t6, "D4", True) + + # Move T3, then move and handle T5 + protocol.move_labware(t3, "D2", True) + protocol.move_labware(t5, "A3", True) + t5_pickup() + + # Move T6 to On-Deck in B2 and setup, then move to A1 and handle + protocol.move_labware(t6, "B2", True) + t6_setup() + protocol.move_labware(t1, "C3", True) + protocol.move_labware(t6, "A1", True) + t6_pickup() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_LLD.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_LLD.py new file mode 100644 index 00000000000..080fda8a759 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_LLD.py @@ -0,0 +1,93 @@ +from opentrons import protocol_api + +# metadata +metadata = { + "protocolName": "2.20 Error Recovery Testing Protocol - 96ch", + "author": "Sara Kowalski", + "description": "Simple Protocol that user can use to Phase 1 Error Recovery options for a 96 channel p1000.", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + +DRYRUN = "NO" + + +def run(protocol: protocol_api.ProtocolContext): + + # modules/fixtures + trashbin = protocol.load_trash_bin(location="A3") + + # labware + tip_adapter = protocol.load_adapter("opentrons_flex_96_tiprack_adapter", "A2") + tiprack1 = tip_adapter.load_labware("opentrons_flex_96_tiprack_1000ul") + sample_plate = protocol.load_labware("armadillo_96_wellplate_200ul_pcr_full_skirt", "C3") + reservoir = protocol.load_labware("nest_1_reservoir_290ml", "D3") + wet_sample = protocol.load_labware("nest_12_reservoir_15ml", "B3") + + # liquids + water = protocol.define_liquid( + name="Water", + description="water for ER testing", + display_color="#90e0ef", + ) + waterButMoreBlue = protocol.define_liquid( + name="Water but more blue", + description="Water for ER testing", + display_color="#0077b6", + ) + + wet_sample["A1"].load_liquid(liquid=water, volume=800) + wet_sample["A2"].load_liquid(liquid=waterButMoreBlue, volume=800) + + # instruments + p1000 = protocol.load_instrument("flex_96channel_1000", mount="left", tip_racks=[tiprack1]) + + volume = 900 + + ############################## + #####Tip Pick Up Failure###### + ############################## + + protocol.pause("This tests Tip Pick Up Failure (General Error for now)") + protocol.pause("Please remove tip rack from deck. When you're testing recovery, add tiprack back as necessary.") + p1000.pick_up_tip() + + ########################################## + #####Overpressure - While Aspirating###### + ########################################## + + protocol.pause("Overpressure - While Aspirating") + p1000.home() + protocol.pause("Swap with tip(s) that are hot glued shut (you will need to swap the tips for retry recovery options)") + + for i in range(10): + p1000.aspirate(volume, reservoir["A1"].top()) + p1000.dispense(volume, reservoir["A1"].top()) + + #################################### + #####Liquid Presence Detection###### + #################################### + protocol.pause("This tests Liquid Presence Detection - PLEASE MAKE SURE YOU'RE USING 1000UL TIPS OR YOU WILL BREAK THE PIPETTE") + protocol.pause("Make sure reservoir is empty, and have full reservoir on standby to switch out with for recovery") + + p1000.require_liquid_presence(wet_sample["A1"]) + + p1000.aspirate(volume, wet_sample["A1"]) + p1000.dispense(volume, wet_sample["A1"]) + p1000.home() + + ##################################################### + #####Overpressure - While Dispensing###### + ##################################################### + + protocol.pause("Overpressure - While Dispensing") + + p1000.aspirate(volume, reservoir["A1"].top(20)) + protocol.pause("Swap with tip(s) that are hot glued shut (you will need to cut the tips for retry recovery options)") + p1000.dispense(volume, reservoir["A1"].top(20)) + + p1000.return_tip() + p1000.reset_tipracks() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_COLUMN_HappyPath.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_COLUMN_HappyPath.py new file mode 100644 index 00000000000..6fec53ee5b3 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_COLUMN_HappyPath.py @@ -0,0 +1,121 @@ +from opentrons.protocol_api import COLUMN + +metadata = { + "protocolName": "96Channel COLUMN Happy Path", + "description": "96 channel pipette and a COLUMN partial tip configuration.", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="Partial Tip Rack", + location="B2", + ) + + pipette = protocol.load_instrument(instrument_name="flex_96channel_1000") + + # Which nozzle to use on the pipette + # Think of the nozzles from above like you are looking down on a 96-well plate + # start="A1" Means the West most nozzle column will pickup from the East most column of the tip rack + # start="A12" Means the East most nozzle column will pickup from the West most column of the tip rack + pipette.configure_nozzle_layout( + style=COLUMN, + start="A1", + tip_racks=[partial_tip_rack], + ) + + source_labware = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="Liquid Transfer - Source Labware", + location="C2", + ) + + destination_labware = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="Liquid Transfer - Destination Labware", + location="C3", + ) + + volume = 10 # Default volume for actions that require it + + ############################# + # Pipette do work + + # consolidate does not work with the COLUMN configuration + # consolidate only has a single well as a destination + + # transfer the row + pipette.transfer(volume, source_labware["A1"], destination_labware["A1"]) + + # distribute does not work with the COLUMN configuration + # distribute only has a single well as a source + + pipette.pick_up_tip() + pipette.touch_tip(source_labware["A2"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware["A3"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() + + ############################# + # Change the pipette configuration + + pipette.configure_nozzle_layout( + style=COLUMN, + start="A12", # Which nozzle to start with + tip_racks=[partial_tip_rack], + ) + + ############################# + # Pipette do work + + # consolidate does not work with the COLUMN configuration + # consolidate only has a single well as a destination + + # transfer the row + pipette.transfer(volume, source_labware["A5"], destination_labware["A5"]) + + # distribute does not work with the COLUMN configuration + # distribute only has a single well as a source + + pipette.pick_up_tip() + pipette.touch_tip(source_labware["A6"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware["A7"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_Column3_SINGLE_.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_Column3_SINGLE_.py new file mode 100644 index 00000000000..e5d1427a477 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_Column3_SINGLE_.py @@ -0,0 +1,118 @@ +from opentrons import protocol_api +from opentrons.protocol_api import COLUMN, ALL, SINGLE, ROW + +requirements = {"robotType": "Flex", "apiLevel": "2.20"} + + +def run(protocol: protocol_api.ProtocolContext): + pipette = protocol.load_instrument(instrument_name="flex_96channel_1000") + trash = protocol.load_trash_bin("A1") + t1 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="Partial Tip Rack", + location="A2", + ) + t2 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="Partial Tip Rack", + location="B2", + ) + t3 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="Partial Tip Rack", + location="C2", + ) + t4 = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="Partial Tip Rack", + location="D2", + ) + + ### Prep tipracks in B2 and D2 by removing 3 columns of tips + pipette.configure_nozzle_layout( + style=COLUMN, + start="A1", + tip_racks=[t2], + ) + for i in range(3): + pipette.pick_up_tip() + pipette.drop_tip() + + pipette.configure_nozzle_layout( + style=COLUMN, + start="A1", + tip_racks=[t4], + ) + for i in range(3): + pipette.pick_up_tip() + pipette.drop_tip() + + ### Relocate tipracks to A3 and C3 for single tip extraction at furthest extant + protocol.move_labware(t2, "A3", True) + protocol.move_labware(t4, "C3", True) + + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", + tip_racks=[t2, t4], + ) + + for i in range(72 * 2): + pipette.pick_up_tip() + pipette.drop_tip() + + ### Move tipracks out of the way to B1 and C1 + protocol.move_labware(t2, "B1", True) + protocol.move_labware(t4, "C1", True) + + ### Prepare tiprack in A2 by removing 3 columns of tips + pipette.configure_nozzle_layout( + style=COLUMN, + start="A1", + tip_racks=[t1], + ) + for i in range(3): + pipette.pick_up_tip() + pipette.drop_tip() + + ### Prepare tiprack in C2 by removing bottom 3 rows of tips, plus 15 tips (3 leftmost remaining columns) + ### This results in a tiprack of the following layout (matching the requirements for our bottom right extents): + # X X X X X X X X X - - - + # X X X X X X X X X - - - + # X X X X X X X X X - - - + # X X X X X X X X X - - - + # X X X X X X X X X - - - + # - - - - - - - - - - - - + # - - - - - - - - - - - - + # - - - - - - - - - - - - + pipette.configure_nozzle_layout( + style=ROW, + start="A1", + tip_racks=[t3], + ) + for i in range(3): + pipette.pick_up_tip() + pipette.drop_tip() + + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", + tip_racks=[t3], + ) + for i in range(15): + pipette.pick_up_tip() + pipette.drop_tip() + + ### Relocate tipracks to B3 and D3 on the deck + protocol.move_labware(t1, "B3", True) + protocol.move_labware(t3, "D3", True) + + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", + tip_racks=[t1, t3], + ) + + for i in range(72 + 45): + pipette.pick_up_tip() + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_ROW_HappyPath.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_ROW_HappyPath.py new file mode 100644 index 00000000000..cf253f04bfe --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_ROW_HappyPath.py @@ -0,0 +1,121 @@ +from opentrons.protocol_api import ROW + +metadata = { + "protocolName": "96Channel ROW Happy Path", + "description": "96 channel pipette and a ROW partial tip configuration.", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="Partial Tip Rack", + location="B1", + ) + + pipette = protocol.load_instrument(instrument_name="flex_96channel_1000") + + # Which nozzle to use on the pipette + # Think of the nozzles from above like you are looking down on a 96-well plate + # start="A1" Means the North most nozzle row will pickup from the South most row of the tip rack + # start="H1" Means the South most nozzle row will pickup from the North most row of the tip rack + pipette.configure_nozzle_layout( + style=ROW, + start="A1", + tip_racks=[partial_tip_rack], + ) + + source_labware = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="Liquid Transfer - Source Labware", + location="C2", + ) + + destination_labware = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="Liquid Transfer - Destination Labware", + location="C3", + ) + + volume = 10 # Default volume for actions that require it + + ############################# + # Pipette do work + + # consolidate is does not work with the ROW configuration + # consolidate only has a single well as a destination + + # transfer the row + pipette.transfer(volume, source_labware["A1"], destination_labware["A1"]) + + # distribute does not work with the ROW configuration + # distribute only has a single well as a source + + pipette.pick_up_tip() + pipette.touch_tip(source_labware["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() + + ############################# + # Change the pipette configuration + + pipette.configure_nozzle_layout( + style=ROW, + start="H1", # Which nozzle to start with + tip_racks=[partial_tip_rack], + ) + + ############################# + # Pipette do work + + # consolidate is does not work with the ROW configuration + # consolidate only has a single well as a destination + + # transfer the row + pipette.transfer(volume, source_labware["E1"], destination_labware["E1"]) + + # distribute does not work with the ROW configuration + # distribute only has a single well as a source + + pipette.pick_up_tip() + pipette.touch_tip(source_labware["F1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware["G1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_4Corners50ul.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_4Corners50ul.py new file mode 100644 index 00000000000..1ac96b7a7ac --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_4Corners50ul.py @@ -0,0 +1,66 @@ +from opentrons.protocol_api import SINGLE + +metadata = { + "protocolName": "96Channel SINGLE Pickup on all 4 corners 2 pickups", + "description": "96 2 tips picked up on all 4 corners of the tip rack", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + four_corners = ["A1", "A12", "H1", "H12"] + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_50ul", + label="Partial Tip Rack", + location="C2", + ) + + pipette = protocol.load_instrument(instrument_name="flex_96channel_1000") + + for corner in four_corners: + + pipette.configure_nozzle_layout( + style=SINGLE, + start=corner, + tip_racks=[partial_tip_rack], + ) + + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + protocol.pause("How was the pickup of first tip?") + pipette.drop_tip() + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + protocol.pause("How was the pickup of second tip?") + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathNorthSide.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathNorthSide.py new file mode 100644 index 00000000000..ba26d34802d --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathNorthSide.py @@ -0,0 +1,143 @@ +from opentrons.protocol_api import SINGLE + +metadata = { + "protocolName": "96Channel SINGLE Happy Path H1 or H12", + "description": "Tip Rack North Clearance for the 96 channel pipette and a SINGLE partial tip configuration.", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="Partial Tip Rack", + location="D2", + ) + + pipette = protocol.load_instrument(instrument_name="flex_96channel_1000") + + # Which nozzle to use on the pipette + # Think of the nozzles from above like you are looking down on a 96-well plate + # start="A1" Means the NW nozzle will pickup from the SE corner of the tip rack + # start="A12" Means the SW nozzle will pickup from the NE corner of the tip rack + # start="H1" Means the NE nozzle will pickup from the SW corner of the tip rack + # start="H12" Means the SE nozzle will pickup from the NW corner of the tip rack + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", # Which nozzle to start with + tip_racks=[partial_tip_rack], + ) + + source_labware_C1 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="Liquid Transfer - Source Labware", + location="C1", + ) + + destination_labware_C2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="Liquid Transfer - Destination Labware", + location="C2", + ) + + volume = 10 # Default volume for actions that require it + + ############################# + # Pipette do work + pipette.consolidate( + [volume, volume], + [source_labware_C1["A3"], source_labware_C1["A4"]], + destination_labware_C2["A3"], + ) + + pipette.transfer(volume, source_labware_C1["A6"], source_labware_C1["A6"]) + + pipette.distribute( + 5, + source_labware_C1["A7"], + [destination_labware_C2["A7"], destination_labware_C2["A8"]], + ) + + pipette.pick_up_tip() + pipette.touch_tip(source_labware_C1["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware_C1["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() + + ############################# + # Change the pipette configuration + + pipette.configure_nozzle_layout( + style=SINGLE, + start="H12", # Which nozzle to start with + tip_racks=[partial_tip_rack], + ) + + source_labware_C3 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="C3 Source Labware", + location="C3", + ) + + destination_labware_B2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="B2 Destination Labware", + location="B2", + ) + + ############################# + # Pipette do work + pipette.consolidate( + [volume, volume], + [source_labware_C3["A3"], source_labware_C3["A4"]], + destination_labware_B2["A3"], + ) + + pipette.transfer(volume, source_labware_C3["A6"], destination_labware_B2["A6"]) + + pipette.distribute( + 5, + source_labware_C3["A7"], + [destination_labware_B2["A7"], destination_labware_B2["A8"]], + ) + + pipette.pick_up_tip() + pipette.touch_tip(source_labware_C3["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware_C3["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathSouthSide.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathSouthSide.py new file mode 100644 index 00000000000..e050a0e4558 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_HappyPathSouthSide.py @@ -0,0 +1,143 @@ +from opentrons.protocol_api import SINGLE + +metadata = { + "protocolName": "96Channel SINGLE Happy Path A1 or A12", + "description": "Unsafe protocol ❗❗❗❗❗❗❗❗❗❗❗ will collide with tube.", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_1000ul", + label="Partial Tip Rack", + location="A2", + ) + + pipette = protocol.load_instrument(instrument_name="flex_96channel_1000") + + # Which nozzle to use on the pipette + # Think of the nozzles from above like you are looking down on a 96-well plate + # start="A1" Means the NW nozzle will pickup from the SE corner of the tip rack + # start="A12" Means the SW nozzle will pickup from the NE corner of the tip rack + # start="H1" Means the NE nozzle will pickup from the SW corner of the tip rack + # start="H12" Means the SE nozzle will pickup from the NW corner of the tip rack + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", # Which nozzle to start with + tip_racks=[partial_tip_rack], + ) + + source_labware_C1 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="C1 Source Labware", + location="C1", + ) + + destination_labware_C2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="C2 Destination Labware", + location="C2", + ) + + volume = 10 # Default volume for actions that require it + + ############################# + # Pipette do work + pipette.consolidate( + [volume, volume], + [source_labware_C1["A3"], source_labware_C1["A4"]], + destination_labware_C2["A3"], + ) + + pipette.transfer(volume, source_labware_C1["A6"], destination_labware_C2["A6"]) + + pipette.distribute( + 5, + source_labware_C1["A7"], + [destination_labware_C2["A7"], destination_labware_C2["A8"]], + ) + + pipette.pick_up_tip() + pipette.touch_tip(source_labware_C1["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware_C1["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() + + ############################# + # Change the pipette configuration + + pipette.configure_nozzle_layout( + style=SINGLE, + start="A12", # Which nozzle to start with + tip_racks=[partial_tip_rack], + ) + + source_labware_B2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="B2 Source Labware", + location="B2", + ) + + destination_labware_C3 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="C3 Destination Labware", + location="C3", + ) + + ############################# + # Pipette do work + pipette.consolidate( + [volume, volume], + [source_labware_B2["A3"], source_labware_B2["A4"]], + destination_labware_C3["A3"], + ) + + pipette.transfer(volume, source_labware_B2["A6"], destination_labware_C3["A6"]) + + pipette.distribute( + 5, + source_labware_B2["A7"], + [destination_labware_C3["A7"], destination_labware_C3["A8"]], + ) + + pipette.pick_up_tip() + pipette.touch_tip(source_labware_B2["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware_B2["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_TubeRackCollision.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_TubeRackCollision.py new file mode 100644 index 00000000000..b499a0ef16d --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_None_SINGLE_TubeRackCollision.py @@ -0,0 +1,68 @@ +from opentrons.protocol_api import SINGLE + +metadata = { + "protocolName": "96Channel SINGLE Pickup tuberack collision", + "description": "Unsafe protocol ❗❗❗❗❗❗❗❗❗❗❗ will collide with tube.", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_50ul", + label="Partial Tip Rack", + location="B2", + ) + + # tubes in column 1 and 2 are taller than the tubes in 3 and 4 + tube_rack = protocol.load_labware( + load_name="opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical", + label="Tube Rack", + location="D2", + ) + + pipette = protocol.load_instrument(instrument_name="flex_96channel_1000") + + pipette.configure_nozzle_layout( + style=SINGLE, + start="H12", + tip_racks=[partial_tip_rack], + ) + + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + # tubes in column 1 and 2 are taller than the tubes in 3 and 4 + # I would expect that with 50ul tips + # the pipette would collide with the tubes in column 1 and 2 + pipette.aspirate(30, tube_rack["B3"].bottom()) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_Reservoir.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_Reservoir.py new file mode 100644 index 00000000000..399c0008d10 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_96_Reservoir.py @@ -0,0 +1,91 @@ +from opentrons.protocol_api import SINGLE + +metadata = { + "protocolName": "96Channel Partial Tip to single well labware", + "description": "How does the 96 channel pipette behave with a partial tip rack and a single well labware?", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def aspirate_to_reservoir_test(ctx, labware): + location = labware.wells()[0] + ctx.comment(f"Aspirating from {labware.parent} {location}") + ctx.aspirate(20, location) + ctx.pause("Where did I aspirate from?") + + +def run(protocol): + + trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_flex_96_tiprack_200ul", + label="Partial Tip Rack", + location="B2", + ) + + # agilent_1_reservoir_290ml + agilent_290 = protocol.load_labware( + load_name="agilent_1_reservoir_290ml", + label="Agilent 290mL", + location="D1", + ) + + # axygen_1_reservoir_90ml + axygen_90 = protocol.load_labware( + load_name="axygen_1_reservoir_90ml", + label="Axygen 90mL", + location="D2", + ) + + # nest_1_reservoir_195ml + nest_195 = protocol.load_labware( + load_name="nest_1_reservoir_195ml", + label="Nest 195mL", + location="D3", + ) + + pipette = protocol.load_instrument(instrument_name="flex_96channel_1000") + + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", + tip_racks=[partial_tip_rack], + ) + + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + aspirate_to_reservoir_test(protocol, agilent_290) + + pipette.configure_nozzle_layout( + style=COLUMN, + start="A1", + tip_racks=[partial_tip_rack], + ) diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_LPD.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_LPD.py new file mode 100644 index 00000000000..c2038880a03 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_LPD.py @@ -0,0 +1,122 @@ +import random +from typing import Set +from opentrons import protocol_api + +# metadata +metadata = { + "protocolName": "Wet test for LPD", + "author": "Josh McVey", + "description": "http://sandbox.docs.opentrons.com/edge/v2/pipettes/loading.html#liquid-presence-detection", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def load_liquid_in_all_wells(labware, liquid) -> None: + for well in labware.wells(): + well.load_liquid(liquid=liquid, volume=well.max_volume) + + +def run(protocol: protocol_api.ProtocolContext): + + # modules/fixtures + trashbin = protocol.load_trash_bin(location="A3") + + # labware + tiprack1 = protocol.load_labware("opentrons_flex_96_tiprack_1000ul", "A2") + tiprack2 = protocol.load_labware("opentrons_flex_96_tiprack_50ul", "B2") + sample_plate = protocol.load_labware("armadillo_96_wellplate_200ul_pcr_full_skirt", "C3") + reservoir = protocol.load_labware("nest_1_reservoir_290ml", "D3") + wet_sample = protocol.load_labware("nest_12_reservoir_15ml", "B3") + + # liquids + waterButMoreBlue = protocol.define_liquid( + name="H20", + description="Test this wet!!!", + display_color="#0077b6", + ) + + load_liquid_in_all_wells(wet_sample, waterButMoreBlue) + + # instruments + p50 = protocol.load_instrument("flex_1channel_50", mount="right", tip_racks=[tiprack2], liquid_presence_detection=True) + volume = 50 + + pipette = p50 + + # Wet A1 to sample plate A1 + # should be successful + well = "A1" + pipette.pick_up_tip() + pipette.aspirate(volume, wet_sample.well(well)) + pipette.dispense(volume, sample_plate.well(well)) + pipette.drop_tip() + + # reuse a tip with liquid_presence_detection=True + # we do NOT get an error if we reuse a tip + # but it is not recommended + well = "A2" + pipette.pick_up_tip() + pipette.aspirate(volume, wet_sample.well(well)) + pipette.dispense(volume, sample_plate.well(well)) + + well = "A3" + pipette.aspirate(volume, wet_sample.well(well)) + pipette.dispense(volume, sample_plate.well(well)) + pipette.drop_tip() + + # disable liquid presence detection on the pipette + pipette.liquid_presence_detection = False + # dry aspirate to prove it is off + protocol.comment(f"Reservoir in {reservoir.parent} is to have NO liquid") + pipette.pick_up_tip() + # dry aspirate to prove it is off + pipette.aspirate(volume, reservoir["A1"]) + pipette.blow_out(trashbin) # make sure tip is empty then use for next step + protocol.comment(f"Current volume in pipette: {pipette.current_volume}") # prints 0 + # detect liquid presence + # we expect this to move the pipette to well A1 of the reservoir + # and then return False during the run + # but will always return true in the simulation (analysis) + # ❗❗❗❗❗❗ + # currently the next line is throwing an error: + # Error 4000 GENERAL_ERROR (ProtocolCommandFailedError): TipNotEmptyError: + # This operation requires a tip with no liquid in it. + # https://opentrons.atlassian.net/browse/RQA-3171 + pipette.prepare_to_aspirate() # This removes the error + is_liquid_in_reservoir = pipette.detect_liquid_presence(reservoir["A1"]) + protocol.comment(f"Is there liquid in the reservoir? {is_liquid_in_reservoir}") + if not protocol.is_simulating(): + if is_liquid_in_reservoir: + protocol.comment("🐛🐛🐛🐛🐛 False + for liquid") + pipette.drop_tip() + # ❗❗❗❗❗❗ + + # now we turn back on liquid presence detection + # with the property + pipette.liquid_presence_detection = True + pipette.pick_up_tip() + # the next line should throw an error and pause the protocol + # resolve and continue + protocol.comment(f"We expect an error on the next line") + pipette.aspirate(volume, reservoir["A1"]) + + if pipette.has_tip: + pipette.drop_tip() + + # pipette.liquid_presence_detection = True + # and we try to use + # pipette.detect_liquid_presence + # no error is thrown regardless of the presence of liquid + + pipette.pick_up_tip() + protocol.comment(f"Reservoir in {reservoir.parent} is to have NO liquid") + pipette.detect_liquid_presence(reservoir["A1"]) + is_liquid_in_reservoir = pipette.detect_liquid_presence(reservoir["A1"]) + protocol.comment(f"Is there liquid in the reservoir? {is_liquid_in_reservoir}") + if not protocol.is_simulating(): + if is_liquid_in_reservoir: + protocol.comment("🐛🐛🐛🐛🐛 False + for liquid") diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_LPD_wet_tip_scenarios.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_LPD_wet_tip_scenarios.py new file mode 100644 index 00000000000..059d4278001 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_LPD_wet_tip_scenarios.py @@ -0,0 +1,169 @@ +import random +from typing import Set +from opentrons import protocol_api + +# metadata +metadata = { + "protocolName": "LPD with wet tip scenarios", + "author": "Josh McVey", + "description": "http://sandbox.docs.opentrons.com/edge/v2/pipettes/loading.html#liquid-presence-detection", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def load_liquid_in_all_wells(labware, liquid) -> None: + for well in labware.wells(): + well.load_liquid(liquid=liquid, volume=well.max_volume) + + +def run(protocol: protocol_api.ProtocolContext): + + # modules/fixtures + trashbin = protocol.load_trash_bin(location="A3") + + # labware + tiprack1 = protocol.load_labware("opentrons_flex_96_tiprack_1000ul", "A2") + tiprack2 = protocol.load_labware("opentrons_flex_96_tiprack_50ul", "B2") + sample_plate = protocol.load_labware("armadillo_96_wellplate_200ul_pcr_full_skirt", "C3") + reservoir = protocol.load_labware("nest_1_reservoir_290ml", "D3") + wet_sample = protocol.load_labware("nest_12_reservoir_15ml", "B3") + + # liquids + waterButMoreBlue = protocol.define_liquid( + name="H20", + description="Test this wet!!!", + display_color="#0077b6", + ) + + wet_sample.well("A1").load_liquid(liquid=waterButMoreBlue, volume=200) + wet_sample.well("A2").load_liquid(liquid=waterButMoreBlue, volume=200) + wet_sample.well("A3").load_liquid(liquid=waterButMoreBlue, volume=200) + + # instruments + p50 = protocol.load_instrument("flex_1channel_50", mount="right", tip_racks=[tiprack2], liquid_presence_detection=True) + volume = 50 + + pipette = p50 + total_volume = 30 + + pipette.pick_up_tip() + protocol.comment("touch_tip") + pipette.touch_tip(wet_sample.well("A1")) + protocol.comment("air_gap") + pipette.air_gap(volume=20) + protocol.comment("blow_out with no arguments") + pipette.blow_out() + pipette.drop_tip() + + protocol.comment(f"reuse=True") + pipette.transfer( + volume=10, + source=[wet_sample.well("A1"), wet_sample.well("A2"), wet_sample.well("A3")], + dest=sample_plate.well("A2"), + reuse=True, + ) + + # example_1 = "During mixing, all aspirates on repetitions > 1 LPD with wet tip" + # protocol.comment(f"{example_1} 🔽🔽🔽🔽🔽🔽🔽") + # pipette.pick_up_tip() + # pipette.mix(repetitions=3, volume=20, location=wet_sample.well("A1")) + # pipette.drop_tip() + # protocol.comment(f"{example_1} 🔼🔼🔼🔼🔼🔼🔼") + + # example_2 = "Consolidate default, reuse=true, new_tip once or never;second and subsequent aspirates LPD with wet tip" + # protocol.comment(f"{example_2} 🔽🔽🔽🔽🔽🔽🔽") + # protocol.comment(f"default tip use") + # pipette.consolidate( + # volume=total_volume, + # source=[wet_sample.well("A1"), wet_sample.well("A2"), wet_sample.well("A3")], + # dest=sample_plate.well("A1"), + # ) + # protocol.comment(f"reuse=True") + # pipette.consolidate( + # volume=total_volume, + # source=[wet_sample.well("A1"), wet_sample.well("A2"), wet_sample.well("A3")], + # dest=sample_plate.well("A2"), + # reuse=True, + # ) + # protocol.comment(f"new_tip='once'") + # pipette.consolidate( + # volume=total_volume, + # source=[wet_sample.well("A1"), wet_sample.well("A2"), wet_sample.well("A3")], + # dest=sample_plate.well("A3"), + # new_tip="once", + # ) + # protocol.comment(f"new_tip='never'") + # pipette.pick_up_tip() + # pipette.consolidate( + # volume=total_volume, + # source=[wet_sample.well("A1"), wet_sample.well("A2"), wet_sample.well("A3")], + # dest=sample_plate.well("A4"), + # new_tip="never", + # ) + # pipette.drop_tip() + # protocol.comment(f"{example_2} 🔼🔼🔼🔼🔼🔼🔼") + + # example_3 = "Consolidate mix_before or mix_after, during mixing, all aspirates on repetitions > 1 LPD with wet tip" + # protocol.comment(f"{example_3} 🔽🔽🔽🔽🔽🔽🔽") + # pipette.consolidate( + # volume=total_volume, + # source=[wet_sample.well("A1"), wet_sample.well("A2"), wet_sample.well("A3")], + # dest=sample_plate.well("A5"), + # mix_before=(3, 15), + # mix_after=(3, 20), + # ) + # protocol.comment(f"{example_3} 🔼🔼🔼🔼🔼🔼🔼") + + # example_4 = "Distribute default and reuse=true;second and subsequent aspirates LPD with wet tip" + # protocol.comment(f"{example_4} 🔽🔽🔽🔽🔽🔽🔽") + # pipette.distribute( + # volume=total_volume, + # source=sample_plate.well("A2"), + # dest=[sample_plate.well("A1"), sample_plate.well("A3")], + # reuse=True, + # ) + # protocol.comment(f"{example_4} 🔼🔼🔼🔼🔼🔼🔼") + + # example_5 = "Distribute mix=true, During mixing, all aspirates on repetitions > 1 LPD with wet tip" + # protocol.comment(f"{example_5} 🔽🔽🔽🔽🔽🔽🔽") + # pipette.distribute( + # volume=total_volume, + # source=sample_plate.well("A2"), + # dest=[sample_plate.well("A1"), sample_plate.well("A3")], + # mix=True, + + # # reuse the tip for a second transfer + # # no error + # # we must say in docs do not do this + # protocol.comment("LPD no error thrown on simple command tip reuse 🔽🔽🔽🔽🔽🔽🔽") + # well = "A2" + # pipette.pick_up_tip() + # pipette.aspirate(volume, wet_sample.well(well)) + # pipette.dispense(volume, sample_plate.well(well)) + + # well = "A3" + # pipette.aspirate(volume, wet_sample.well(well)) + # pipette.dispense(volume, sample_plate.well(well)) + # pipette.drop_tip() + # protocol.comment("LPD no error thrown on simple command tip reuse 🔼🔼🔼🔼🔼🔼🔼") + + # # Again no error is thrown + # # but we must say in docs do not do this + # protocol.comment("LPD no error thrown on blowout + prepare_to_aspirate 🔽🔽🔽🔽🔽🔽🔽") + # pipette.pick_up_tip() + # pipette.aspirate(volume=20, location=reservoir["A1"]) + # pipette.blow_out(trashbin) # make sure tip is empty then use for next step + # protocol.comment(f"Current volume in pipette: {pipette.current_volume}") # prints 0 + # # pipette.prepare_to_aspirate() removes the error from line 93 + # # but this should NOT be done!!! + # # Error 4000 GENERAL_ERROR (ProtocolCommandFailedError): TipNotEmptyError: + # # This operation requires a tip with no liquid in it. + # pipette.prepare_to_aspirate() + # is_liquid_in_reservoir = pipette.detect_liquid_presence(reservoir["A1"]) + # protocol.comment(f"Is there liquid in the reservoir? {is_liquid_in_reservoir}") + # pipette.drop_tip() + # protocol.comment("LPD no error thrown on blowout + prepare_to_aspirate 🔼🔼🔼🔼🔼🔼🔼") diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_touch_tip_directly.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_touch_tip_directly.py new file mode 100644 index 00000000000..ac8a4420650 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P50_touch_tip_directly.py @@ -0,0 +1,66 @@ +import random +from typing import Set +from opentrons import protocol_api + +# metadata +metadata = { + "protocolName": "Flex touch tip first", + "author": "Josh McVey", + "description": "touch tip first", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +def load_liquid_in_all_wells(labware, liquid) -> None: + for well in labware.wells(): + well.load_liquid(liquid=liquid, volume=well.max_volume) + + +def run(protocol: protocol_api.ProtocolContext): + + # modules/fixtures + trashbin = protocol.load_trash_bin(location="A3") + + # labware + tiprack1 = protocol.load_labware("opentrons_flex_96_tiprack_1000ul", "A2") + tiprack2 = protocol.load_labware("opentrons_flex_96_tiprack_50ul", "B2") + sample_plate = protocol.load_labware("armadillo_96_wellplate_200ul_pcr_full_skirt", "C3") + reservoir = protocol.load_labware("nest_1_reservoir_290ml", "D3") + wet_sample = protocol.load_labware("nest_12_reservoir_15ml", "B3") + + # liquids + waterButMoreBlue = protocol.define_liquid( + name="H20", + description="Test this wet!!!", + display_color="#0077b6", + ) + + wet_sample.well("A1").load_liquid(liquid=waterButMoreBlue, volume=200) + wet_sample.well("A2").load_liquid(liquid=waterButMoreBlue, volume=200) + wet_sample.well("A3").load_liquid(liquid=waterButMoreBlue, volume=200) + + # instruments + p50 = protocol.load_instrument("flex_1channel_50", mount="right", tip_racks=[tiprack2], liquid_presence_detection=True) + volume = 50 + + pipette = p50 + total_volume = 30 + + pipette.pick_up_tip() + # don't do an aspirate before the touch_tip + # pipette.aspirate(volume=total_volume, location=wet_sample.well("A1")) + protocol.comment("touch_tip") + # no matter if you aspirate before or not, + # the touch_tip is not shown in the app preview run + pipette.touch_tip(location=wet_sample.well("A1")) + protocol.comment("air_gap") + # if you uncomment the air_gap an error is thrown + # I should be at the wet_sample.well("A1") but it says I am at the tiprack + # pipette.air_gap(volume=20) + protocol.comment("blow_out with no arguments") + pipette.blow_out() + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P8X1000_P50_LLD.py b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P8X1000_P50_LLD.py new file mode 100644 index 00000000000..1550f3313d5 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_S_v2_20_P8X1000_P50_LLD.py @@ -0,0 +1,106 @@ +from opentrons import protocol_api + +# metadata +metadata = { + "protocolName": "2.20 Error Recovery Testing Protocol - Modified", + "author": "Sara Kowalski", + "description": "Simple Protocol that user can use to Phase 1 Error Recovery options for single and multi channel pipettes. NOTE: YOU WILL NEED A MODIFIED PIPETTE (NO SHROUD), and changed order of operations to reduce impact of dispense ER failure", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + +DRYRUN = "NO" + + +def run(protocol: protocol_api.ProtocolContext): + + # modules/fixtures + trashbin = protocol.load_trash_bin(location="A3") + + # labware + tiprack1 = protocol.load_labware("opentrons_flex_96_tiprack_1000ul", "A2") + tiprack2 = protocol.load_labware("opentrons_flex_96_tiprack_50ul", "B2") + sample_plate = protocol.load_labware("armadillo_96_wellplate_200ul_pcr_full_skirt", "C3") + reservoir = protocol.load_labware("nest_1_reservoir_290ml", "D3") + wet_sample = protocol.load_labware("nest_12_reservoir_15ml", "B3") + + # liquids + water = protocol.define_liquid( + name="Water", + description="water for ER testing", + display_color="#90e0ef", + ) + waterButMoreBlue = protocol.define_liquid( + name="Water but more blue", + description="Water for ER testing", + display_color="#0077b6", + ) + + wet_sample["A1"].load_liquid(liquid=water, volume=800) + wet_sample["A2"].load_liquid(liquid=waterButMoreBlue, volume=800) + + # instruments + p1000 = protocol.load_instrument( + "flex_8channel_1000", mount="left", tip_racks=[tiprack1] + ) ## will need a modified pipette that doesn't have the ejector(?) + p50 = protocol.load_instrument( + "flex_1channel_50", mount="right", tip_racks=[tiprack2] + ) ## will need a modified pipette that doesn't have the ejector(?) + + pipette_list = [p1000, p50] + volume = 900 + + for pipette in pipette_list: + if pipette == p50: + volume = 50 + + ############################## + #####Tip Pick Up Failure###### + ############################## + + protocol.pause("This tests Tip Pick Up Failure (General Error for now)") + protocol.pause("Please remove tip rack from deck. When you're testing recovery, add tiprack back as necessary.") + pipette.pick_up_tip() + + ########################################## + #####Overpressure - While Aspirating###### + ########################################## + + protocol.pause("Overpressure - While Aspirating") + pipette.home() + protocol.pause( + "Use p50UL tips - Swap with tip(s) that are hot glued shut (you will need to swap the tips for retry recovery options)" + ) + + for i in range(10): + pipette.aspirate(volume, reservoir["A1"].top()) + pipette.dispense(volume, reservoir["A1"].top()) + + #################################### + #####Liquid Presence Detection###### + #################################### + protocol.pause("This tests Liquid Presence Detection - PLEASE MAKE SURE YOU'RE USING 1000UL TIPS OR YOU WILL BREAK THE PIPETTE") + protocol.pause("Make sure reservoir is empty, and have full reservoir on standby to switch out with for recovery") + + pipette.require_liquid_presence(wet_sample["A1"]) + + pipette.aspirate(volume, wet_sample["A1"]) + pipette.dispense(volume, wet_sample["A1"]) + pipette.home() + + ##################################################### + #####Overpressure - While Dispensing###### + ##################################################### + + protocol.pause("Overpressure - While Dispensing") + + pipette.aspirate(volume, reservoir["A1"].top(20)) + protocol.pause("Swap with tip(s) that are hot glued shut (you will need to cut the tips for retry recovery options)") + pipette.dispense(volume, reservoir["A1"].top(20)) + + pipette.move_to(trashbin) + protocol.pause("You will have to manually remove tips if you're using a modified pipette") + pipette.drop_tip(trashbin) diff --git a/analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_None_Overrides_TooTallLabware.py b/analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_None_Overrides_TooTallLabware.py new file mode 100644 index 00000000000..d717c497655 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_None_Overrides_TooTallLabware.py @@ -0,0 +1,504 @@ +from dataclasses import dataclass +from typing import Optional +from opentrons.protocol_api import SINGLE, COLUMN, PARTIAL_COLUMN, ROW + +metadata = { + "protocolName": "Too tall labware on pickup tip", + "description": "oooo", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +@dataclass +class PartialTipConfig: + key: str + description: str + startingTip: str + startingNozzle: str + apiTipConfig: str + apiStart: str + apiEnd: Optional[str] + + +# flex_96channel_1000 SINGLE + +ninety_six_single_top_left = PartialTipConfig( + key="ninety_six_single_top_left", + description="96 single picking up top left of tiprack", + startingTip="A1", + startingNozzle="H12", + apiTipConfig=SINGLE, + apiStart="H12", + apiEnd=None, +) + +ninety_six_single_top_right = PartialTipConfig( + key="ninety_six_single_top_right", + description="96 single picking up top right of tiprack", + startingTip="A12", + startingNozzle="H1", + apiTipConfig=SINGLE, + apiStart="H1", + apiEnd=None, +) + +ninety_six_single_bottom_left = PartialTipConfig( + key="ninety_six_single_bottom_left", + description="96 single picking up bottom left of tiprack", + startingTip="H1", + startingNozzle="A12", + apiTipConfig=SINGLE, + apiStart="A12", + apiEnd=None, +) + +ninety_six_single_bottom_right = PartialTipConfig( + key="ninety_six_single_bottom_right", + description="96 single picking up bottom right of tiprack", + startingTip="H12", + startingNozzle="A1", + apiTipConfig=SINGLE, + apiStart="A1", + apiEnd=None, +) + +# flex_96channel_1000 COLUMN + +ninety_six_column_left = PartialTipConfig( + key="ninety_six_column_left", + description="96 column picking up left column of tiprack", + startingTip="Column 1", + startingNozzle="Column 12", + apiTipConfig=COLUMN, + apiStart="A12", + apiEnd=None, +) + + +ninety_six_column_right = PartialTipConfig( + key="ninety_six_column_right", + description="96 column picking up right column of tiprack", + startingTip="Row 12", + startingNozzle="Row 1", + apiTipConfig=COLUMN, + apiStart="A1", + apiEnd=None, +) + +# flex_96channel_1000 ROW + +ninety_six_row_top = PartialTipConfig( + key="ninety_six_row_top", + description="96 row picking up top row of tiprack", + startingTip="Row A", + startingNozzle="Row H", + apiTipConfig=ROW, + apiStart="H1", + apiEnd=None, +) + +ninety_six_row_bottom = PartialTipConfig( + key="ninety_six_row_bottom", + description="96 row picking up bottom row of tiprack", + startingTip="Row H", + startingNozzle="Row A", + apiTipConfig=ROW, + apiStart="A1", + apiEnd=None, +) + +# pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="right") +# works for all 8 channel pipettes +eight_single_top = PartialTipConfig( + key="eight_single_top", + description="8 channel single picking up from the top of the tiprack", + startingTip="A1", + startingNozzle="H1", + apiTipConfig=SINGLE, + apiStart="H1", + apiEnd=None, +) + +eight_single_bottom = PartialTipConfig( + key="eight_single_bottom", + description="8 channel single picking up from the bottom of the tiprack", + startingTip="H1", + startingNozzle="A1", + apiTipConfig=SINGLE, + apiStart="A1", + apiEnd=None, +) + + +eight_partial_top_2_tips = PartialTipConfig( + key="eight_partial_top_2_tips", + description="8 partial bottom 2 tips", + startingTip="H1", + startingNozzle="B1", + apiTipConfig=PARTIAL_COLUMN, + apiStart="H1", + apiEnd="B1", +) + +eight_partial_top_3_tips = PartialTipConfig( + key="eight_partial_top_3_tips", + description="8 partial bottom 3 tips", + startingTip="H1", + startingNozzle="C1", + apiTipConfig=PARTIAL_COLUMN, + apiStart="H1", + apiEnd="C1", +) +eight_partial_top_4_tips = PartialTipConfig( + key="eight_partial_top_4", + description="8 partial bottom 4 tips", + startingTip="H1", + startingNozzle="D1", + apiTipConfig=PARTIAL_COLUMN, + apiStart="H1", + apiEnd="D1", +) +eight_partial_top_5_tips = PartialTipConfig( + key="eight_partial_top_5", + description="8 partial bottom 5 tips", + startingTip="H1", + startingNozzle="E1", + apiTipConfig=PARTIAL_COLUMN, + apiStart="H1", + apiEnd="E1", +) +eight_partial_top_6_tips = PartialTipConfig( + key="eight_partial_top_6", + description="8 partial bottom 6 tips", + startingTip="H1", + startingNozzle="F1", + apiTipConfig=PARTIAL_COLUMN, + apiStart="H1", + apiEnd="F1", +) + +eight_partial_top_7_tips = PartialTipConfig( + key="eight_partial_top_7", + description="8 partial bottom 7 tips", + startingTip="H1", + startingNozzle="G1", + apiTipConfig=PARTIAL_COLUMN, + apiStart="H1", + apiEnd="G1", +) + +all_partial_configs = [ + ninety_six_single_top_left, + ninety_six_single_top_right, + ninety_six_single_bottom_left, + ninety_six_single_bottom_right, + ninety_six_column_left, + ninety_six_column_right, + ninety_six_row_top, + ninety_six_row_bottom, + eight_single_top, + eight_single_bottom, + eight_partial_top_2_tips, + eight_partial_top_3_tips, + eight_partial_top_4_tips, + eight_partial_top_5_tips, + eight_partial_top_6_tips, + eight_partial_top_7_tips, +] + + +def find_partial_tip_config(key: str) -> Optional[PartialTipConfig]: + for config in all_partial_configs: + if config.key == key: + return config + raise ValueError(f"Could not find partial tip config with key {key}") + + +@dataclass +class TestCase: + key: str + description: str + pipette_config_key: str + collision_slot: Optional[str] = None + tip_rack_slot: Optional[str] = None + movement: Optional[str] = None + source_slot: Optional[str] = None + source_well: Optional[str] = None + destination_slot: Optional[str] = None + destination_well: Optional[str] = None + + +north = TestCase( + key="north", + description="North too tall labware on pickup tip", + pipette_config_key="ninety_six_single_top_left", + collision_slot="B2", +) + +north_west = TestCase( + key="north_west", + description="NW too tall labware on pickup tip", + pipette_config_key="ninety_six_single_top_left", + collision_slot="B1", +) + +west = TestCase( + key="west", + description="west too tall labware on pickup tip", + pipette_config_key="ninety_six_single_top_left", + collision_slot="C1", +) + +south_west = TestCase( + key="south_west", + description="west too tall labware on pickup tip", + pipette_config_key="ninety_six_single_bottom_left", + collision_slot="D1", +) + +south = TestCase( + key="south", + description="west too tall labware on pickup tip", + pipette_config_key="ninety_six_single_bottom_left", + collision_slot="D2", +) + +south_east = TestCase( + key="south_east", + description="west too tall labware on pickup tip", + pipette_config_key="ninety_six_single_bottom_right", + collision_slot="D3", +) +east = TestCase( + key="east", + description="west too tall labware on pickup tip", + pipette_config_key="ninety_six_single_bottom_right", + collision_slot="C3", +) + +east_column = TestCase( + key="east_column", + description="west too tall labware on pickup tip", + pipette_config_key="ninety_six_column_left", + collision_slot="C1", +) +west_column = TestCase( + key="west_column", + description="west too tall labware on pickup tip", + pipette_config_key="ninety_six_column_right", + collision_slot="C3", +) + +north_row = TestCase( + key="north_row", + description="north row too tall labware on pickup tip", + pipette_config_key="ninety_six_row_top", + collision_slot="B2", +) + +south_row = TestCase( + key="south_row", + description="south row too tall labware on pickup tip", + pipette_config_key="ninety_six_row_bottom", + collision_slot="D2", +) + +top_edge = TestCase(key="top_edge", description="top edge of robot", pipette_config_key="ninety_six_single_top_left", tip_rack_slot="A1") + +bottom_left_edge = TestCase( + key="bottom_left_edge", description="bottom left edge of robot", pipette_config_key="ninety_six_single_bottom_left", tip_rack_slot="D1" +) + +bottom_right_edge = TestCase( + key="bottom_right_edge", + description="bottom right edge of robot", + pipette_config_key="ninety_six_single_bottom_right", + tip_rack_slot="D3", +) + +c3_right_edge = TestCase( + key="c3_right_edge", description="right edge of c2", pipette_config_key="ninety_six_single_bottom_right", tip_rack_slot="C3" +) + +transfer_destination_collision = TestCase( + key="transfer_destination_collision", + description="transfer north", + pipette_config_key="ninety_six_single_top_left", + tip_rack_slot="B2", + source_slot="D2", + source_well="A1", + destination_slot="D3", + destination_well="A1", + movement="transfer", + collision_slot="C3", +) + +transfer_source_collision = TestCase( + key="transfer_source_collision", + description="transfer north", + pipette_config_key="ninety_six_single_top_left", + tip_rack_slot="B2", + source_slot="D2", + source_well="A1", + destination_slot="D3", + destination_well="A1", + movement="transfer", + collision_slot="C1", +) + +mix_collision = TestCase( + key="mix_collision", + description="mix north", + pipette_config_key="ninety_six_single_top_left", + tip_rack_slot="B2", + source_slot="D2", + source_well="A1", + movement="mix", + collision_slot="C1", +) + +consolidate_source_collision = TestCase( + key="consolidate_source_collision", + description="consolidate north", + pipette_config_key="ninety_six_single_top_left", + tip_rack_slot="B2", + source_slot="D2", + source_well="A1", + destination_slot="D3", + destination_well="A1", + movement="consolidate", + collision_slot="C1", +) + +consolidate_destination_collision = TestCase( + key="consolidate_destination_collision", + description="consolidate north", + pipette_config_key="ninety_six_single_top_left", + tip_rack_slot="B2", + source_slot="D2", + source_well="A1", + destination_slot="D3", + destination_well="A1", + movement="consolidate", + collision_slot="C3", +) + +distribute_source_collision = TestCase( + key="distribute_source_collision", + description="distribute north", + pipette_config_key="ninety_six_single_top_left", + tip_rack_slot="B2", + source_slot="D2", + source_well="A1", + destination_slot="D3", + destination_well="A1", + movement="distribute", + collision_slot="C1", +) + +distribute_destination_collision = TestCase( + key="distribute_destination_collision", + description="distribute north", + pipette_config_key="ninety_six_single_top_left", + tip_rack_slot="B2", + source_slot="D2", + source_well="A1", + destination_slot="D3", + destination_well="A1", + movement="distribute", + collision_slot="C3", +) + +# all have been tested manually and throw an error as expected JTM 20240814 +test_cases = [ + transfer_source_collision, + transfer_destination_collision, + c3_right_edge, + north, + north_west, + west, + south_west, + south, + south_east, + east, + east_column, + west_column, + north_row, + south_row, + top_edge, + bottom_left_edge, + bottom_left_edge, + bottom_right_edge, + mix_collision, + consolidate_source_collision, + consolidate_destination_collision, + distribute_source_collision, + distribute_destination_collision, +] + + +def get_test_case(key: str) -> Optional[TestCase]: + for test_case in test_cases: + if test_case.key == key: + return test_case + raise ValueError(f"Could not find test case with key {key}") + + +def run(ctx): + tall_labware_loadname = "opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical" + test_case = get_test_case(key) + + if test_case.tip_rack_slot and test_case.tip_rack_slot != "C2": + tip_rack = ctx.load_labware("opentrons_96_tiprack_1000ul", test_case.tip_rack_slot) + else: + tip_rack = ctx.load_labware("opentrons_96_tiprack_1000ul", "C2") + + pipette_config = find_partial_tip_config(test_case.pipette_config_key) + + pipette = ctx.load_instrument("flex_96channel_1000") + + pipette.configure_nozzle_layout(pipette_config.apiTipConfig, pipette_config.apiStart, tip_racks=[tip_rack]) + + if test_case.collision_slot: + ctx.load_labware(tall_labware_loadname, test_case.collision_slot) + + target_labware_loadname = "nest_96_wellplate_100ul_pcr_full_skirt" + if test_case.source_slot: + source = ctx.load_labware(target_labware_loadname, test_case.source_slot) + + if test_case.destination_slot: + destination = ctx.load_labware(target_labware_loadname, test_case.destination_slot) + + if not test_case.movement: + None # No movement simply pickup tip from the tip rack + pipette.pick_up_tip() + elif test_case.movement == "transfer": + trash = ctx.load_trash_bin("A3") + pipette.transfer(10, source[test_case.source_well], destination[test_case.destination_well]) + elif test_case.movement == "mix": + trash = ctx.load_trash_bin("A3") + well = source[test_case.source_well] + pipette.pick_up_tip() + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.drop_tip() + elif test_case.movement == "consolidate": + trash = ctx.load_trash_bin("A3") + pipette.consolidate( + [10, 10], + [source[test_case.source_well], source[test_case.source_well]], + destination[test_case.destination_well], + ) + elif test_case.movement == "distribute": + trash = ctx.load_trash_bin("A3") + pipette.distribute( + 20, + source[test_case.source_well], + [destination[test_case.destination_well], destination[test_case.destination_well]], + ) diff --git a/analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs.py b/analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs.py new file mode 100644 index 00000000000..9d275c2db84 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs.py @@ -0,0 +1,185 @@ +from dataclasses import dataclass +from typing import Optional +from opentrons.protocol_api import PARTIAL_COLUMN + +# inspired by https://opentrons.atlassian.net/browse/PLAT-457 + +metadata = { + "protocolName": "Invalid tip configs that should error", + "description": "oooo", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + + +@dataclass +class PartialTipConfig: + key: str + pipette_load_name: str + description: str + starting_tip: str + starting_nozzle: str + api_tip_config: str + api_start: str + api_end: Optional[str] = None + + +# Want to see +"Partial column configuration is only supported on 8-Channel pipettes" +ninety_six_partial_column_1 = PartialTipConfig( + key="ninety_six_partial_column_1", + pipette_load_name="flex_96channel_1000", + description="96 2 tip pick up top left of tiprack", + starting_tip="A1", + starting_nozzle="H12", + api_tip_config=PARTIAL_COLUMN, + api_start="H12", + api_end="G12", +) + +# https://opentrons.atlassian.net/browse/PLAT-457 +ninety_six_partial_column_2 = PartialTipConfig( + key="ninety_six_partial_column_2", + pipette_load_name="flex_96channel_1000", + description="Full row", + starting_tip="A1", + starting_nozzle="H12", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="A1", +) + +ninety_six_partial_column_3 = PartialTipConfig( + key="ninety_six_partial_column_3", + pipette_load_name="flex_96channel_1000", + description="Full Row", + starting_tip="A1", + starting_nozzle="H12", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="H12", +) + +# We do not allow PARTIAL_COLUMN to start on the bottom of the tip rack +# Want to see +# "IncompatibleNozzleConfiguration: Attempted Nozzle Configuration does not match any approved map layout for the current pipette." +eight_partial_column_bottom_left = PartialTipConfig( + key="eight_partial_column_bottom_left", + pipette_load_name="flex_8channel_1000", + description="8 channel 2 tip pick up bottom left of tiprack", + starting_tip="H1", + starting_nozzle="A1", + api_tip_config=PARTIAL_COLUMN, + api_start="A1", + api_end="B1", +) + + +# Want to see +# Error 4000 GENERAL_ERROR (ProtocolCommandFailedError): IncompatibleNozzleConfiguration: No entry for front right nozzle 'G12' in pipette +eight_partial_column_bottom_right = PartialTipConfig( + key="eight_partial_column_bottom_right", + pipette_load_name="flex_8channel_1000", + description="8 channel 2 tip pick up bottom left of tiprack", + starting_tip="H12", + starting_nozzle="A1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", # for partial column only H1 + api_end="G12", # the author thinks this is to specify the ending tip and wants to start at bottom right for 2 tips +) + + +# Partial column configurations require the 'end' parameter. +eight_partial_column_no_end = PartialTipConfig( + key="eight_partial_column_no_end", + pipette_load_name="flex_8channel_1000", + description="8 channel PARTIAL_COLUMN with no end", + starting_tip="H1", + starting_nozzle="A1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + # api_end="B1", sets the end to None +) + +# If you call return_tip() while using partial tip pickup, the API will raise an error. +# Error 4000 GENERAL_ERROR (UnexpectedProtocolError): Cannot return tip to a tiprack while the pipette is configured for partial tip. +return_tip_error = PartialTipConfig( + key="return_tip_error", + pipette_load_name="flex_8channel_1000", + description="8 channel 2 tip pick up top left of tiprack", + starting_tip="A1", + starting_nozzle="H1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="G1", # valid 2 tip +) + + +# pipette.drop_tip(tiprack["B1"]) # drops tip in rack location A1 +drop_tip_with_location = PartialTipConfig( + key="drop_tip_with_location", + pipette_load_name="flex_8channel_1000", + description="8 channel 2 tip pick up top left of tiprack", + starting_tip="A1", + starting_nozzle="H1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="G1", # valid 2 tip +) + +all_partial_configs = [ + ninety_six_partial_column_1, + ninety_six_partial_column_2, + ninety_six_partial_column_3, + eight_partial_column_bottom_left, + eight_partial_column_bottom_right, + eight_partial_column_no_end, + return_tip_error, + drop_tip_with_location, +] + + +def find_partial_tip_config(key: str) -> Optional[PartialTipConfig]: + for config in all_partial_configs: + if config.key == key: + return config + raise ValueError(f"Could not find partial tip config with key {key}") + + +def comment_column_has_tip(ctx, tip_rack, column): + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + wells = [f"{row}{column}" for row in range_A_to_H] + for well in wells: + ctx.comment(f"Tip rack in {tip_rack.parent}, well {well} has tip: {tip_rack.wells_by_name()[well].has_tip}") + + +def run(ctx): + tip_rack = ctx.load_labware("opentrons_flex_96_tiprack_1000ul", "B2") + + pipette_config = find_partial_tip_config(key) + + pipette = ctx.load_instrument(pipette_config.pipette_load_name, "left") + + pipette.configure_nozzle_layout( + style=pipette_config.api_tip_config, start=pipette_config.api_start, end=pipette_config.api_end, tip_racks=[tip_rack] + ) + + target_labware_loadname = "nest_96_wellplate_100ul_pcr_full_skirt" + source = ctx.load_labware(target_labware_loadname, "D2") + destination = ctx.load_labware(target_labware_loadname, "D3") + + trash = ctx.load_trash_bin("A3") + if key == "return_tip_error": + pipette.pick_up_tip() + # this test picks up 2 tips + comment_column_has_tip(ctx, tip_rack, 1) + pipette.return_tip() # this should raise an error + elif key == "drop_tip_with_location": + pipette.pick_up_tip() + comment_column_has_tip(ctx, tip_rack, 1) + pipette.drop_tip(tip_rack["B1"]) # this should raise an error + else: + pipette.transfer(10, source["A1"], destination["A1"]) diff --git a/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_PARTIAL_COLUMN_HappyPathMixedTipRacks.py b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_PARTIAL_COLUMN_HappyPathMixedTipRacks.py new file mode 100644 index 00000000000..784667691d4 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_PARTIAL_COLUMN_HappyPathMixedTipRacks.py @@ -0,0 +1,111 @@ +from opentrons import protocol_api +from opentrons.protocol_api import PARTIAL_COLUMN + +metadata = { + "protocolName": "Partial Tip with Partial Column and Single Smoke", + "description": "OT-2 protocol with 1ch and 8ch pipette partial/single tip configurations. Mixing tipracks and using separate tipracks. ", +} + +requirements = { + "robotType": "OT-2", + "apiLevel": "2.20", +} + + +def run(protocol: protocol_api.ProtocolContext): + + # DECK SETUP AND LABWARE + partial_tiprack_1 = protocol.load_labware("opentrons_96_tiprack_300ul", "7") + partial_tiprack_2 = protocol.load_labware("opentrons_96_tiprack_300ul", "8") + sample_plate = protocol.load_labware("opentrons_96_wellplate_200ul_pcr_full_skirt", "2") + reservoir_12 = protocol.load_labware("nest_12_reservoir_15ml", "1") + reservoir_1 = protocol.load_labware("nest_1_reservoir_290ml", "3") + + p300_multi = protocol.load_instrument("p300_multi_gen2", mount="left", tip_racks=[partial_tiprack_1]) + p300_single = protocol.load_instrument("p300_single_gen2", mount="right", tip_racks=[partial_tiprack_2]) + + p300_multi.configure_nozzle_layout(style=PARTIAL_COLUMN, start="H1", end="D1", tip_racks=[partial_tiprack_1]) + p300_multi.pick_up_tip() + p300_multi.mix(3, 75, sample_plate["E1"]) + p300_multi.mix(3, 200, reservoir_12["A1"]) + p300_multi.mix(3, 200, reservoir_1["A1"]) + p300_multi.drop_tip() + + p300_multi.transfer( + volume=150, + source=sample_plate["E1"], ##NOTE TO SELF THINK ABOUT THIS THE OFFSET SHOULD BE + dest=sample_plate["E12"], + new_tip="once", + ) + + p300_multi.configure_nozzle_layout(style=PARTIAL_COLUMN, start="H1", end="E1", tip_racks=[partial_tiprack_1]) + p300_multi.distribute( + volume=300, + source=reservoir_12["A12"], + dest=[reservoir_12["A11"], reservoir_12["A10"], reservoir_12["A9"], reservoir_12["A8"], reservoir_12["A7"], reservoir_12["A6"]], + ) + + p300_multi.configure_nozzle_layout(style=PARTIAL_COLUMN, start="H1", end="F1", tip_racks=[partial_tiprack_1]) + p300_multi.consolidate( + volume=25, + source=[ + reservoir_12["A1"], + reservoir_12["A2"], + reservoir_12["A3"], + reservoir_12["A4"], + reservoir_12["A5"], + reservoir_12["A6"], + ], + dest=reservoir_1["A1"], + ) + + p300_multi.pick_up_tip() + p300_multi.touch_tip(reservoir_12["A7"]) + p300_multi.drop_tip() + p300_multi.pick_up_tip() + p300_multi.home() + p300_multi.drop_tip() + + p300_multi.pick_up_tip() + well = reservoir_12["A7"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + p300_multi.move_to(well.bottom(z=2)) + p300_multi.mix(10, 10) + p300_multi.move_to(well.top(z=5)) + p300_multi.blow_out() + p300_multi.prepare_to_aspirate() + p300_multi.move_to(well.bottom(z=2)) + p300_multi.aspirate(10, well.bottom(z=2)) + p300_multi.dispense(10) + p300_multi.drop_tip() + + p300_single.transfer(volume=25, source=sample_plate["A1"], dest=sample_plate["A12"], new_tip="once") + p300_single.distribute( + volume=300, + source=reservoir_12["A1"], + dest=[reservoir_12["A2"], reservoir_12["A3"], reservoir_12["A4"], reservoir_12["A5"], reservoir_12["A6"], reservoir_12["A7"]], + ) + + p300_single.consolidate( + volume=25, + source=[ + reservoir_12["A6"], + reservoir_12["A7"], + reservoir_12["A8"], + reservoir_12["A9"], + reservoir_12["A10"], + reservoir_12["A11"], + reservoir_12["A12"], + ], + dest=reservoir_1["A1"], + ) + + try: + while partial_tiprack_1 != None: + for well in sample_plate.wells(): + p300_single.pick_up_tip(partial_tiprack_1) + p300_single.aspirate(10, well) + p300_single.dispense(10, well) + p300_single.drop_tip() + except: + p300_single.home() diff --git a/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_SINGLE_HappyPath.py b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_SINGLE_HappyPath.py new file mode 100644 index 00000000000..1adf454421c --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_SINGLE_HappyPath.py @@ -0,0 +1,135 @@ +from opentrons.protocol_api import SINGLE + +metadata = { + "protocolName": "OT2 8 Channel SINGLE Happy Path A1 and H1", + "description": "OT2 8 Channel pipette and a SINGLE partial tip configuration.", +} + +requirements = { + "robotType": "OT-2", + "apiLevel": "2.20", +} + + +def run(protocol): + + # trash = protocol.load_trash_bin("A3") # must load trash bin + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_96_tiprack_300ul", + label="Partial Tip Rack", + location="8", + ) + + pipette = protocol.load_instrument(instrument_name="p300_multi_gen2", mount="left") + # mount on the right and you will get an error. + + # On the 8-channel SINGLE + # start="A1" Means the North nozzle will pickup from the SW corner of the tip rack + # start="H1" Means the South nozzle will pickup from the NW corner of the tip rack + pipette.configure_nozzle_layout( + style=SINGLE, + start="A1", # Which nozzle + tip_racks=[partial_tip_rack], + ) + + source_labware_2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="2 Source Labware", + location="2", + ) + + destination_labware_3 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="3 Destination Labware", + location="3", + ) + + volume = 10 # Default volume for actions that require it + + ############################# + # Pipette do work + pipette.consolidate( + [volume, volume], + [source_labware_2["A3"], source_labware_2["A4"]], + destination_labware_3["A3"], + ) + + pipette.transfer(volume, source_labware_2["A6"], destination_labware_3["A6"]) + + pipette.distribute( + 5, + source_labware_2["A7"], + [destination_labware_3["A7"], destination_labware_3["A8"]], + ) + + pipette.pick_up_tip() + pipette.touch_tip(source_labware_2["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = source_labware_2["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() + + ############################ + # Change the pipette configuration + + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", # Which nozzle + tip_racks=[partial_tip_rack], + ) + + source_labware_4 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="4 Source Labware", + location="4", + ) + + ############################# + # Pipette do work + pipette.consolidate( + [volume, volume], + [source_labware_4["A3"], source_labware_4["A4"]], + destination_labware_3["A3"], + ) + + pipette.transfer(volume, source_labware_4["A6"], source_labware_4["A6"]) + + pipette.distribute( + 5, + source_labware_4["A7"], + [destination_labware_3["A7"], destination_labware_3["A8"]], + ) + + pipette.pick_up_tip() + pipette.touch_tip(destination_labware_3["B1"]) + pipette.drop_tip() + pipette.pick_up_tip() + pipette.home() + pipette.drop_tip() + + pipette.pick_up_tip() + well = destination_labware_3["D1"] + # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate + pipette.move_to(well.bottom(z=2)) + pipette.mix(10, 10) + pipette.move_to(well.top(z=5)) + pipette.blow_out() + pipette.prepare_to_aspirate() + pipette.move_to(well.bottom(z=2)) + pipette.aspirate(10, well.bottom(z=2)) + pipette.dispense(10) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_SINGLE_TubeRackCollision.py b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_SINGLE_TubeRackCollision.py new file mode 100644 index 00000000000..2cf77913071 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_8_None_SINGLE_TubeRackCollision.py @@ -0,0 +1,64 @@ +from opentrons.protocol_api import SINGLE + +metadata = { + "protocolName": "8Channel SINGLE Pickup Tube Rack collision", + "description": "Unsafe protocol ❗❗❗❗❗❗❗❗❗❗❗ will collide with tube.", +} + +requirements = { + "robotType": "OT-2", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_96_tiprack_300ul", + label="Partial Tip Rack", + location="3", + ) + + tube_rack = protocol.load_labware( + load_name="opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical", + label="Tube Rack", + location="2", + ) + + pipette = protocol.load_instrument(instrument_name="p300_multi_gen2", mount="left") + + pipette.configure_nozzle_layout( + style=SINGLE, + start="H1", + tip_racks=[partial_tip_rack], + ) + + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + # both commands are not physically possible but throw no error + pipette.aspirate(30, tube_rack["A3"].bottom()) + pipette.dispense(30, tube_rack["A4"].bottom()) + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P20M_PARTIAL_COLUMN_Overhang.py b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P20M_PARTIAL_COLUMN_Overhang.py new file mode 100644 index 00000000000..6e4a3b5b8e5 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P20M_PARTIAL_COLUMN_Overhang.py @@ -0,0 +1,155 @@ +from opentrons.protocol_api import PARTIAL_COLUMN + +metadata = { + "protocolName": "p20_multi_gen2 PARTIAL_COLUMN Overhang", + "description": "A protocol that demonstrates overhang into a slot with a partial column configuration.", + "author": "Josh McVey", +} + +requirements = { + "robotType": "OT-2", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_96_tiprack_20ul", + label="Partial Tip Rack", + location="10", + ) + + pipette = protocol.load_instrument(instrument_name="p20_multi_gen2", mount="left") + + pipette.configure_nozzle_layout( + style=PARTIAL_COLUMN, + start="H1", + end="D1", # 5 Tips + tip_racks=[partial_tip_rack], + ) + + source_labware_5 = protocol.load_labware( + load_name="nest_1_reservoir_290ml", + label="Source Reservoir", + location="5", + ) + + destination_labware_2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="PCR Plate", + location="2", + ) + + # Known issue in 8.0.0 + # if you target A1 of the labware in 2 + # nozzle H1 - the front-most nozzle will go to A1 + # this means that tips will be out of the slot + # they will over hang into the 5 slot + # no error is raised + # the overhanging tips will collide with the labware in slot 5 + + # If we ever do detect and error for this + # Take all these examples into a negative Overrides test + + volume = 5 + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + # why with P20 do I get: + # MOTION_PLANNING_FAILURE (PartialTipMovementNotAllowedError): Moving to PCR Plate in slot 2 + # with H1 nozzle partial configuration will result in collision with items in deck slot 5. + # because the P20 would actually collide with the labware in slot 5 + # https://opentrons.atlassian.net/browse/RQA-3198 + # bad - 4 tips will overhang into slot 5 + # pipette.aspirate(volume=volume, location=destination_labware_2["A1"]) + # bad - 3 tips will overhang into slot 5 + # pipette.aspirate(volume=volume, location=destination_labware_2["B1"]) + # bad - 2 tips will overhang into slot 5 + # pipette.aspirate(volume=volume, location=destination_labware_2["C1"]) + # bad - 1 tip will overhang into slot 5 + # pipette.aspirate(volume=volume, location=destination_labware_2["D1"]) + # pipette.aspirate(volume=volume, location=destination_labware_2["E1"]) + # pipette.aspirate(volume=volume, location=destination_labware_2["F1"]) + # pipette.aspirate(volume=volume, location=destination_labware_2["G1"]) + # H is the only safe row + pipette.aspirate(volume=volume, location=destination_labware_2["H1"]) + + +# ignore the below for the time being +""" + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # bad - bad - 4 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["A1"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # bad - bad - 3 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["B2"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # bad - bad - 2 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["C3"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # bad - bad - 1 tip will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["D4"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # this is safe - 0 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["E5"]) + # bad - has overhang into B2 - 4 tips + pipette.touch_tip(location=destination_labware_2["A1"]) + # bad - has overhang into B2 - 4 tips + pipette.blow_out(location=destination_labware_2["A1"]) + # bad - has overhang into B2 - 4 tips + pipette.mix(repetitions=3, volume=volume, location=destination_labware_2["A2"]) + # bad - aspirate and dispense have overhang into B2 + pipette.drop_tip() + pipette.transfer(volume=volume, source=destination_labware_2["A1"], dest=destination_labware_2["A2"]) + # bad - aspirate has 3 tip overhang but not dispense + pipette.transfer(volume=volume, source=destination_labware_2["B1"], dest=destination_labware_2["E1"]) + # bad - aspirate is safe but dispense has 2 tip overhang + pipette.transfer(volume=volume, source=destination_labware_2["E1"], dest=destination_labware_2["C3"]) + # bad - source and destinations have overhang + pipette.distribute(volume=20, source=destination_labware_2["D2"], dest=[destination_labware_2["D3"], destination_labware_2["D4"]]) + # bad - source has overhang but destinations are safe + pipette.distribute(volume=20, source=destination_labware_2["D2"], dest=[destination_labware_2["E3"], destination_labware_2["E4"]]) + # bad - source has no overhang but 1 destination does + pipette.distribute(volume=20, source=destination_labware_2["E6"], dest=[destination_labware_2["A7"], destination_labware_2["E7"]]) + # bad - source has no overhang but 2 destinations do + pipette.distribute(volume=20, source=destination_labware_2["E7"], dest=[destination_labware_2["A8"], destination_labware_2["A9"]]) + # bad - all sources and destination have overhang + pipette.consolidate(volume=volume, source=[destination_labware_2["A9"], destination_labware_2["A10"]], dest=destination_labware_2["A11"]) + # bad - 1 source has overhang but destination is safe + pipette.consolidate(volume=volume, source=[destination_labware_2["A9"], destination_labware_2["E10"]], dest=destination_labware_2["E11"]) + # bad - sources are safe but destination has overhang + pipette.consolidate(volume=volume, source=[destination_labware_2["E9"], destination_labware_2["E10"]], dest=destination_labware_2["A12"]) +""" diff --git a/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P20M_Simple.py b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P20M_Simple.py new file mode 100644 index 00000000000..6fcfad51541 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P20M_Simple.py @@ -0,0 +1,90 @@ +from opentrons.protocol_api import PARTIAL_COLUMN + +metadata = { + "protocolName": "p20_multi_gen2 Simple", + "description": "A protocol that demonstrates safe actions with p20_multi_gen2", + "author": "Josh McVey", +} + +requirements = { + "robotType": "OT-2", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + tip_rack = protocol.load_labware( + load_name="opentrons_96_tiprack_20ul", + label="Partial Tip Rack", + location="10", + ) + + pipette = protocol.load_instrument(instrument_name="p20_multi_gen2", mount="left", tip_racks=[tip_rack]) + + source_labware_5 = protocol.load_labware( + load_name="nest_1_reservoir_290ml", + label="Source Reservoir", + location="5", + ) + + destination_labware_2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="PCR Plate", + location="2", + ) + + volume = 10 + pipette.pick_up_tip() + comment_tip_rack_status(protocol, tip_rack) + pipette.aspirate(volume=volume, location=destination_labware_2["H1"]) + pipette.dispense(volume=volume, location=destination_labware_2["H2"]) + for i in range(1, 13): + protocol.comment(f"Touching tip to {destination_labware_2[f'H{i}']}") + pipette.touch_tip(location=destination_labware_2[f"H{i}"]) + + pipette.blow_out(location=destination_labware_2["H1"]) + pipette.mix(repetitions=3, volume=volume, location=destination_labware_2["H1"]) + pipette.drop_tip() + # Note that you cannot target Hn like you could on a partial tip + # Invalid source for multichannel transfer: [H3 of PCR Plate on slot 2] + # pipette.transfer(volume=volume, source=destination_labware_2["H3"], dest=destination_labware_2["H4"]) + # comment_tip_rack_status(protocol, tip_rack) + # Invalid source for multichannel transfer: [H4 of PCR Plate on slot 2] + # pipette.distribute(volume=volume, source=destination_labware_2["H4"], dest=[destination_labware_2["H5"], destination_labware_2["H6"]]) + # comment_tip_rack_status(protocol, tip_rack) + # Invalid source for multichannel transfer: [H7 of PCR Plate on slot 2, H8 of PCR Plate on slot 2] + # pipette.consolidate(volume=volume, source=[destination_labware_2["H7"], destination_labware_2["H8"]], dest=destination_labware_2["H9"]) + # comment_tip_rack_status(protocol, tip_rack) + + # but this works and I am inferring + # when using 8 channel pipette and you are specifying a column, use An + pipette.transfer(volume=volume, source=destination_labware_2["A3"], dest=destination_labware_2["A4"]) + comment_tip_rack_status(protocol, tip_rack) + pipette.distribute(volume=volume, source=destination_labware_2["A4"], dest=[destination_labware_2["A5"], destination_labware_2["A6"]]) + comment_tip_rack_status(protocol, tip_rack) + pipette.consolidate(volume=volume, source=[destination_labware_2["A7"], destination_labware_2["A8"]], dest=destination_labware_2["A9"]) + comment_tip_rack_status(protocol, tip_rack) diff --git a/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P300M_PARTIAL_COLUMN_Overhang.py b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P300M_PARTIAL_COLUMN_Overhang.py new file mode 100644 index 00000000000..489b72abfe5 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P300M_PARTIAL_COLUMN_Overhang.py @@ -0,0 +1,151 @@ +from opentrons.protocol_api import PARTIAL_COLUMN + +metadata = { + "protocolName": "p300_multi_gen2 PARTIAL_COLUMN Overhang", + "description": "A protocol that demonstrates overhang into a slot with a partial column configuration.", + "author": "Josh McVey", +} + +requirements = { + "robotType": "OT-2", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + partial_tip_rack = protocol.load_labware( + load_name="opentrons_96_tiprack_300ul", + label="Partial Tip Rack", + location="10", + ) + + pipette = protocol.load_instrument(instrument_name="p300_multi_gen2", mount="left") + + pipette.configure_nozzle_layout( + style=PARTIAL_COLUMN, + start="H1", + end="D1", # 5 Tips + tip_racks=[partial_tip_rack], + ) + + source_labware_5 = protocol.load_labware( + load_name="nest_1_reservoir_290ml", + label="Source Reservoir", + location="5", + ) + + destination_labware_2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="PCR Plate", + location="2", + ) + + # Known issue in 8.0.0 + # if you target A1 of the labware in 2 + # nozzle H1 - the front-most nozzle will go to A1 + # this means that tips will be out of the slot + # they will over hang into the 5 slot + # no error is raised + # the overhanging tips will collide with the labware in 5 + + # If we ever do detect and error for this + # Take all these examples into a negative Overrides test + + volume = 20 + pipette.pick_up_tip() + comment_tip_rack_status(protocol, partial_tip_rack) + # bad - 4 tips will overhang into slot 5 + pipette.aspirate(volume=volume, location=destination_labware_2["A1"]) + # bad - 3 tips will overhang into slot 5 + pipette.aspirate(volume=volume, location=destination_labware_2["B1"]) + # bad - 2 tips will overhang into slot 5 + pipette.aspirate(volume=volume, location=destination_labware_2["C1"]) + # bad - 1 tip will overhang into slot 5 + pipette.aspirate(volume=volume, location=destination_labware_2["D1"]) + # these should be safe??? + pipette.aspirate(volume=volume, location=destination_labware_2["E1"]) + pipette.aspirate(volume=volume, location=destination_labware_2["F1"]) + pipette.aspirate(volume=volume, location=destination_labware_2["G1"]) + # only one that is safe + pipette.aspirate(volume=volume, location=destination_labware_2["H1"]) + + +# ignore the below for the time being +""" + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # bad - bad - 4 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["A1"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # bad - bad - 3 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["B2"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # bad - bad - 2 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["C3"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # bad - bad - 1 tip will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["D4"]) + # this is safe - from a reservoir + # must aspirate before dispense + pipette.aspirate(volume=volume, location=source_labware_5["A1"]) + # this is safe - 0 tips will overhang into B2 + pipette.dispense(volume=volume, location=destination_labware_2["E5"]) + # bad - has overhang into B2 - 4 tips + pipette.touch_tip(location=destination_labware_2["A1"]) + # bad - has overhang into B2 - 4 tips + pipette.blow_out(location=destination_labware_2["A1"]) + # bad - has overhang into B2 - 4 tips + pipette.mix(repetitions=3, volume=volume, location=destination_labware_2["A2"]) + # bad - aspirate and dispense have overhang into B2 + pipette.drop_tip() + pipette.transfer(volume=volume, source=destination_labware_2["A1"], dest=destination_labware_2["A2"]) + # bad - aspirate has 3 tip overhang but not dispense + pipette.transfer(volume=volume, source=destination_labware_2["B1"], dest=destination_labware_2["E1"]) + # bad - aspirate is safe but dispense has 2 tip overhang + pipette.transfer(volume=volume, source=destination_labware_2["E1"], dest=destination_labware_2["C3"]) + # bad - source and destinations have overhang + pipette.distribute(volume=20, source=destination_labware_2["D2"], dest=[destination_labware_2["D3"], destination_labware_2["D4"]]) + # bad - source has overhang but destinations are safe + pipette.distribute(volume=20, source=destination_labware_2["D2"], dest=[destination_labware_2["E3"], destination_labware_2["E4"]]) + # bad - source has no overhang but 1 destination does + pipette.distribute(volume=20, source=destination_labware_2["E6"], dest=[destination_labware_2["A7"], destination_labware_2["E7"]]) + # bad - source has no overhang but 2 destinations do + pipette.distribute(volume=20, source=destination_labware_2["E7"], dest=[destination_labware_2["A8"], destination_labware_2["A9"]]) + # bad - all sources and destination have overhang + pipette.consolidate(volume=volume, source=[destination_labware_2["A9"], destination_labware_2["A10"]], dest=destination_labware_2["A11"]) + # bad - 1 source has overhang but destination is safe + pipette.consolidate(volume=volume, source=[destination_labware_2["A9"], destination_labware_2["E10"]], dest=destination_labware_2["E11"]) + # bad - sources are safe but destination has overhang + pipette.consolidate(volume=volume, source=[destination_labware_2["E9"], destination_labware_2["E10"]], dest=destination_labware_2["A12"]) +""" diff --git a/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P300M_Simple.py b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P300M_Simple.py new file mode 100644 index 00000000000..7728a94a077 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P300M_Simple.py @@ -0,0 +1,94 @@ +from opentrons.protocol_api import PARTIAL_COLUMN, ALL + +metadata = { + "protocolName": "p20_multi_gen2 Simple", + "description": "A protocol that demonstrates safe actions with p20_multi_gen2", + "author": "Josh McVey", +} + +requirements = { + "robotType": "OT-2", + "apiLevel": "2.20", +} + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(protocol): + + tip_rack = protocol.load_labware( + load_name="opentrons_96_tiprack_300ul", + label="Partial Tip Rack", + location="10", + ) + + pipette = protocol.load_instrument(instrument_name="p300_multi_gen2", mount="left", tip_racks=[tip_rack]) + + source_labware_5 = protocol.load_labware( + load_name="nest_1_reservoir_290ml", + label="Source Reservoir", + location="5", + ) + + destination_labware_2 = protocol.load_labware( + load_name="nest_96_wellplate_100ul_pcr_full_skirt", + label="PCR Plate", + location="2", + ) + + volume = 40 + pipette.pick_up_tip() + comment_tip_rack_status(protocol, tip_rack) + pipette.aspirate(volume=volume, location=destination_labware_2["H1"]) + pipette.dispense(volume=volume, location=destination_labware_2["H2"]) + for i in range(1, 13): + protocol.comment(f"Touching tip to {destination_labware_2[f'H{i}']}") + pipette.touch_tip(location=destination_labware_2[f"H{i}"]) + + pipette.blow_out(location=destination_labware_2["H1"]) + pipette.mix(repetitions=3, volume=volume, location=destination_labware_2["H1"]) + pipette.drop_tip() + + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + column1 = [destination_labware_2[f"{row}1"] for row in range_A_to_H] + column2 = [destination_labware_2[f"{row}2"] for row in range_A_to_H] + protocol.comment(f"Transferring {volume}uL from column 1 to column 2") + pipette.transfer(volume=volume, source=column1, dest=column2) + comment_tip_rack_status(protocol, tip_rack) + + # Note that you cannot target Hn like you could on a partial tip + # when I try to use the well destination of Hn for + # transfer, distribute, or consolidate, I get an error like + # Invalid source for multichannel transfer: [H3 of PCR Plate on slot 2] + # pipette.transfer(volume=volume, source=destination_labware_2["H3"], dest=destination_labware_2["H4"]) + # comment_tip_rack_status(protocol, tip_rack) + + # but this works and I am inferring + # when using 8 channel pipette and you are specifying a column, use An + pipette.transfer(volume=volume, source=destination_labware_2["A3"], dest=destination_labware_2["A4"]) + comment_tip_rack_status(protocol, tip_rack) + pipette.distribute(volume=volume, source=destination_labware_2["A4"], dest=[destination_labware_2["A5"], destination_labware_2["A6"]]) + comment_tip_rack_status(protocol, tip_rack) + pipette.consolidate(volume=volume, source=[destination_labware_2["A7"], destination_labware_2["A8"]], dest=destination_labware_2["A9"]) + comment_tip_rack_status(protocol, tip_rack) diff --git a/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P50_touch_tip.py b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P50_touch_tip.py new file mode 100644 index 00000000000..50f04c1ead2 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/OT2_S_v2_20_P50_touch_tip.py @@ -0,0 +1,52 @@ +import random +from typing import Set +from opentrons import protocol_api + +# metadata +metadata = { + "protocolName": "touch_tip directly on OT2", + "author": "Josh McVey", + "description": "touch tip on OT2", +} + +requirements = { + "robotType": "OT-2", + "apiLevel": "2.20", +} + + +def load_liquid_in_all_wells(labware, liquid) -> None: + for well in labware.wells(): + well.load_liquid(liquid=liquid, volume=well.max_volume) + + +def run(protocol: protocol_api.ProtocolContext): + + # labware + tiprack2 = protocol.load_labware("opentrons_96_tiprack_20ul", "5") + wet_sample = protocol.load_labware("nest_12_reservoir_15ml", "2") + + # liquids + waterButMoreBlue = protocol.define_liquid( + name="H20", + description="Test this wet!!!", + display_color="#0077b6", + ) + + wet_sample.well("A1").load_liquid(liquid=waterButMoreBlue, volume=200) + wet_sample.well("A2").load_liquid(liquid=waterButMoreBlue, volume=200) + wet_sample.well("A3").load_liquid(liquid=waterButMoreBlue, volume=200) + + # instruments + p20 = protocol.load_instrument("p20_single_gen2", mount="right", tip_racks=[tiprack2]) + + pipette = p20 + + pipette.pick_up_tip() + protocol.comment("touch_tip") + pipette.touch_tip(wet_sample.well("A1")) + protocol.comment("air_gap") + pipette.air_gap(volume=10) + protocol.comment("blow_out with no arguments") + pipette.blow_out() + pipette.drop_tip() diff --git a/analyses-snapshot-testing/files/protocols/OT2_X_v2_20_8_Overrides_InvalidConfigs.py b/analyses-snapshot-testing/files/protocols/OT2_X_v2_20_8_Overrides_InvalidConfigs.py new file mode 100644 index 00000000000..e08cf13eda6 --- /dev/null +++ b/analyses-snapshot-testing/files/protocols/OT2_X_v2_20_8_Overrides_InvalidConfigs.py @@ -0,0 +1,151 @@ +# key = "drop_tip_with_location" +from dataclasses import dataclass +from typing import Optional +from opentrons.protocol_api import PARTIAL_COLUMN + +# inspired by https://opentrons.atlassian.net/browse/PLAT-457 + +metadata = { + "protocolName": "Invalid tip configs that should error", + "description": "oooo", +} + +requirements = { + "robotType": "OT-2", + "apiLevel": "2.20", +} + + +@dataclass +class PartialTipConfig: + key: str + pipette_load_name: str + description: str + starting_tip: str + starting_nozzle: str + api_tip_config: str + api_start: str + api_end: Optional[str] = None + + +# We do not allow PARTIAL_COLUMN to start on the bottom of the tip rack +# Want to see +# "IncompatibleNozzleConfiguration: Attempted Nozzle Configuration does not match any approved map layout for the current pipette." +eight_partial_column_bottom_left = PartialTipConfig( + key="eight_partial_column_bottom_left", + pipette_load_name="p300_multi_gen2", + description="8 channel 2 tip pick up bottom left of tiprack", + starting_tip="H1", + starting_nozzle="A1", + api_tip_config=PARTIAL_COLUMN, + api_start="A1", + api_end="B1", +) + + +# Want to see +# Error 4000 GENERAL_ERROR (ProtocolCommandFailedError): IncompatibleNozzleConfiguration: No entry for front right nozzle 'G12' in pipette +eight_partial_column_bottom_right = PartialTipConfig( + key="eight_partial_column_bottom_right", + pipette_load_name="p20_multi_gen2", + description="8 channel 2 tip pick up bottom left of tiprack", + starting_tip="H12", + starting_nozzle="A1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", # for partial column only H1 + api_end="G12", # the author thinks this is to specify the ending tip and wants to start at bottom right for 2 tips +) + + +# Partial column configurations require the 'end' parameter. +eight_partial_column_no_end = PartialTipConfig( + key="eight_partial_column_no_end", + pipette_load_name="p20_multi_gen2", + description="8 channel PARTIAL_COLUMN with no end", + starting_tip="H1", + starting_nozzle="A1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + # api_end="B1", sets the end to None +) + +# If you call return_tip() while using partial tip pickup, the API will raise an error. +# Error 4000 GENERAL_ERROR (UnexpectedProtocolError): Cannot return tip to a tiprack while the pipette is configured for partial tip. +return_tip_error = PartialTipConfig( + key="return_tip_error", + pipette_load_name="p20_multi_gen2", + description="8 channel 2 tip pick up top left of tiprack", + starting_tip="A1", + starting_nozzle="H1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="G1", # valid 2 tip +) + + +# pipette.drop_tip(tiprack["B1"]) # drops tip in rack location A1 +drop_tip_with_location = PartialTipConfig( + key="drop_tip_with_location", + pipette_load_name="p300_multi_gen2", + description="8 channel 2 tip pick up top left of tiprack", + starting_tip="A1", + starting_nozzle="H1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="G1", # valid 2 tip +) + +all_partial_configs = [ + eight_partial_column_bottom_left, + eight_partial_column_bottom_right, + eight_partial_column_no_end, + return_tip_error, + drop_tip_with_location, +] + + +def find_partial_tip_config(key: str) -> Optional[PartialTipConfig]: + for config in all_partial_configs: + if config.key == key: + return config + raise ValueError(f"Could not find partial tip config with key {key}") + + +def comment_column_has_tip(ctx, tip_rack, column): + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + wells = [f"{row}{column}" for row in range_A_to_H] + for well in wells: + ctx.comment(f"Tip rack in {tip_rack.parent}, well {well} has tip: {tip_rack.wells_by_name()[well].has_tip}") + + +def run(ctx): + + tip_rack_20 = ctx.load_labware("opentrons_96_tiprack_20ul", "1") + tip_rack_300 = ctx.load_labware("opentrons_96_tiprack_300ul", "2") + + pipette_config = find_partial_tip_config(key) + + pipette = ctx.load_instrument(pipette_config.pipette_load_name, "left") + + tip_rack = tip_rack_20 + if pipette_config.pipette_load_name.__contains__("300"): + tip_rack = tip_rack_300 + pipette.configure_nozzle_layout( + style=pipette_config.api_tip_config, start=pipette_config.api_start, end=pipette_config.api_end, tip_racks=[tip_rack] + ) + + target_labware_loadname = "nest_96_wellplate_100ul_pcr_full_skirt" + source = ctx.load_labware(target_labware_loadname, "4") + destination = ctx.load_labware(target_labware_loadname, "5") + + if key == "return_tip_error": + pipette.pick_up_tip() + # this test picks up 2 tips + comment_column_has_tip(ctx, tip_rack, 1) + pipette.return_tip() # this should raise an error + elif key == "drop_tip_with_location": + pipette.pick_up_tip() + comment_column_has_tip(ctx, tip_rack, 1) + pipette.drop_tip(tip_rack["A1"]) # this should raise an error + else: + pipette.transfer(10, source["A1"], destination["A1"]) diff --git a/analyses-snapshot-testing/files/templates/basic_flex_rtp_v1.1.py b/analyses-snapshot-testing/files/templates/basic_flex_rtp_v1.1.py new file mode 100644 index 00000000000..9a35d06e0c4 --- /dev/null +++ b/analyses-snapshot-testing/files/templates/basic_flex_rtp_v1.1.py @@ -0,0 +1,522 @@ +from dataclasses import dataclass +from typing import Any, Optional, Union +from opentrons.protocol_api import SINGLE, COLUMN, PARTIAL_COLUMN, ROW, ALL + +metadata = { + "protocolName": "Basic flex RTP template", +} + +requirements = { + "robotType": "Flex", + "apiLevel": "2.20", +} + +####### RTP DEFINITIONS ####### +# NozzleConfigurationType is from opentrons.hardware_control.nozzle_manager import NozzleConfigurationType +# do not want to import that as that interface or location might change +# type is not in shared-data +# cannot do the below +# ApiTipConfigType = Union[SINGLE, COLUMN, PARTIAL_COLUMN, ROW] + + +@dataclass +class PartialTipConfig: + """Dataclass to hold a partial tip configuration descriptively.""" + + key: str + description: str + starting_tip: str + starting_nozzle: str + api_tip_config: Any + api_start: str + api_end: Optional[str] + + def __str__(self): + return ( + f"🔑 Key: {self.key} | 📝 Description: {self.description} | " + f"💉 Starting Tip: {self.starting_tip} | 🔧 Starting Nozzle: {self.starting_nozzle} | " + f"📜 API Tip Config: {self.api_tip_config} | 🚀 API Start: {self.api_start} | " + f"🛑 API End: {self.api_end if self.api_end else 'None'}" + ) + + +#### Define all viable partial tip configurations. + +# flex_96channel_1000 SINGLE +# names and descriptions describe where relative to the tiprack the pipette will pick up tips + +ninety_six_single_back_left = PartialTipConfig( + key="ninety_six_single_back_left", + description="96 single picking up back left of tiprack", + starting_tip="A1", + starting_nozzle="H12", + api_tip_config=SINGLE, + api_start="H12", + api_end=None, +) + +ninety_six_single_back_right = PartialTipConfig( + key="ninety_six_single_back_right", + description="96 single picking up back right of tiprack", + starting_tip="A12", + starting_nozzle="H1", + api_tip_config=SINGLE, + api_start="H1", + api_end=None, +) + +ninety_six_single_front_left = PartialTipConfig( + key="ninety_six_single_front_left", + description="96 single picking up front left of tiprack", + starting_tip="H1", + starting_nozzle="A12", + api_tip_config=SINGLE, + api_start="A12", + api_end=None, +) + +ninety_six_single_front_right = PartialTipConfig( + key="ninety_six_single_front_right", + description="96 single picking up front right of tiprack", + starting_tip="H12", + starting_nozzle="A1", + api_tip_config=SINGLE, + api_start="A1", + api_end=None, +) + +# flex_96channel_1000 COLUMN + +ninety_six_column_left = PartialTipConfig( + key="ninety_six_column_left", + description="96 column picking up left column of tiprack", + starting_tip="Column 1", + starting_nozzle="Column 12", + api_tip_config=COLUMN, + api_start="A12", + api_end=None, +) + + +ninety_six_column_right = PartialTipConfig( + key="ninety_six_column_right", + description="96 column picking up right column of tiprack", + starting_tip="Row 12", + starting_nozzle="Row 1", + api_tip_config=COLUMN, + api_start="A1", + api_end=None, +) + +# flex_96channel_1000 ROW + +ninety_six_row_back = PartialTipConfig( + key="ninety_six_row_back", + description="96 row picking up back row of tiprack", + starting_tip="Row A", + starting_nozzle="Row H", + api_tip_config=ROW, + api_start="H1", + api_end=None, +) + +ninety_six_row_front = PartialTipConfig( + key="ninety_six_row_front", + description="96 row picking up front row of tiprack", + starting_tip="Row H", + starting_nozzle="Row A", + api_tip_config=ROW, + api_start="A1", + api_end=None, +) + +# 8 channel SINGLE +eight_single = PartialTipConfig( + key="eight_single", + description="8 channel single picking up from the back left of the tiprack", + starting_tip="A1", + starting_nozzle="H1", + api_tip_config=SINGLE, + api_start="H1", + api_end=None, +) + +# PARTIAL_COLUMN +eight_partial_back_7_tips = PartialTipConfig( + key="eight_partial_back_7_tips", + description="8 channel picking up 7 tips", + starting_tip="H1", + starting_nozzle="B1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="B1", +) + +eight_partial_back_6_tips = PartialTipConfig( + key="eight_partial_back_6_tips", + description="8 channel picking up 6 tips", + starting_tip="H1", + starting_nozzle="C1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="C1", +) +eight_partial_back_5_tips = PartialTipConfig( + key="eight_partial_back_5_tips", + description="8 channel picking up 5 tips", + starting_tip="H1", + starting_nozzle="D1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="D1", +) +eight_partial_back_4_tips = PartialTipConfig( + key="eight_partial_back_4_tips", + description="8 channel picking up 4 tips", + starting_tip="H1", + starting_nozzle="E1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="E1", +) +eight_partial_back_3_tips = PartialTipConfig( + key="eight_partial_back_3_tips", + description="8 channel picking up 3 tips", + starting_tip="H1", + starting_nozzle="F1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="F1", +) + +eight_partial_back_2_tips = PartialTipConfig( + key="eight_partial_back_2_tips", + description="8 channel picking up 2 tips", + starting_tip="H1", + starting_nozzle="G1", + api_tip_config=PARTIAL_COLUMN, + api_start="H1", + api_end="G1", +) + +no_tip_config = PartialTipConfig( + key="no_tip_config", + description="Will discard and not set a partial tip config", + starting_tip="", + starting_nozzle="", + api_tip_config=ALL, + api_start="", + api_end="", +) + +# make a list of all the partial tip configurations + +all_partial_configs = [ + ninety_six_single_back_left, + ninety_six_single_back_right, + ninety_six_single_front_left, + ninety_six_single_front_right, + ninety_six_column_left, + ninety_six_column_right, + ninety_six_row_back, + ninety_six_row_front, + eight_single, + eight_partial_back_2_tips, + eight_partial_back_3_tips, + eight_partial_back_4_tips, + eight_partial_back_5_tips, + eight_partial_back_6_tips, + eight_partial_back_7_tips, + no_tip_config, +] + + +def find_partial_tip_config(key: str) -> Optional[PartialTipConfig]: + """Find a partial tip config by key.""" + for config in all_partial_configs: + if config.key == key: + return config + raise ValueError(f"Could not find partial tip config with key {key}") + + +reservoir_choices = [ + { + "display_name": "Agilent Reservoir 290 mL", + "value": "agilent_1_reservoir_290ml", + }, + { + "display_name": "Axygen Reservoir 90 mL", + "value": "axygen_1_reservoir_90ml", + }, + { + "display_name": "nest_12_reservoir_15ml", + "value": "nest_12_reservoir_15ml", + }, + { + "display_name": "Nest Reservoir 195 mL", + "value": "nest_1_reservoir_195ml", + }, + { + "display_name": "Nest Reservoir 290 mL", + "value": "nest_1_reservoir_290ml", + }, + { + "display_name": "usa..._12_reservoir_22ml", + "value": "usascientific_12_reservoir_22ml", + }, +] + +position_choices = [ + {"display_name": "A1", "value": "A1"}, + {"display_name": "A2", "value": "A2"}, + {"display_name": "A3", "value": "A3"}, + {"display_name": "B1", "value": "B1"}, + {"display_name": "B2", "value": "B2"}, + {"display_name": "B3", "value": "B3"}, + {"display_name": "C1", "value": "C1"}, + {"display_name": "C2", "value": "C2"}, + {"display_name": "C3", "value": "C3"}, + {"display_name": "D1", "value": "D1"}, + {"display_name": "D2", "value": "D2"}, + {"display_name": "D3", "value": "D3"}, +] + + +def add_parameters(parameters): + parameters.add_str( + display_name="Partial Tip Configuration", + variable_name="partial_tip_config_key", + default="ninety_six_single_back_left", + description="Partial tip configurations described by pickup nozzle and tip count", + choices=[ # value of each choice maps to the key of the partial tip config dataclass we defined + {"display_name": "96 SINGLE nozzle H12", "value": "ninety_six_single_back_left"}, + {"display_name": "96 SINGLE nozzle H1", "value": "ninety_six_single_back_right"}, + {"display_name": "96 SINGLE nozzle A12", "value": "ninety_six_single_front_left"}, + {"display_name": "96 SINGLE nozzle A1", "value": "ninety_six_single_front_right"}, + {"display_name": "96 COLUMN 1", "value": "ninety_six_column_left"}, + {"display_name": "96 COLUMN 12", "value": "ninety_six_column_right"}, + {"display_name": "96 ROW A", "value": "ninety_six_row_back"}, + {"display_name": "96 ROW H", "value": "ninety_six_row_front"}, + {"display_name": "8 SINGLE", "value": "eight_single"}, + {"display_name": "8 PARTIAL 2 tips", "value": "eight_partial_back_2_tips"}, + {"display_name": "8 PARTIAL 3 tips", "value": "eight_partial_back_3_tips"}, + {"display_name": "8 PARTIAL 4 tips", "value": "eight_partial_back_4_tips"}, + {"display_name": "8 PARTIAL 5 tips", "value": "eight_partial_back_5_tips"}, + {"display_name": "8 PARTIAL 6 tips", "value": "eight_partial_back_6_tips"}, + {"display_name": "8 PARTIAL 7 tips", "value": "eight_partial_back_7_tips"}, + {"display_name": "No Partial tip config", "value": "no_tip_config"}, + ], + ) + + parameters.add_str( + display_name="Pipette", + variable_name="pipette_load_name", + choices=[ + { + "display_name": "50µl single channel", + "value": "flex_1channel_50", + }, + { + "display_name": "1000µl single channel", + "value": "flex_1channel_1000", + }, + { + "display_name": "50µl 8 channel", + "value": "flex_8channel_50", + }, + { + "display_name": "1000µl 8 channel", + "value": "flex_8channel_1000", + }, + { + "display_name": "96-Channel Pipette", + "value": "flex_96channel_1000", + }, + ], + default="flex_96channel_1000", + description="Select the pipette type", + ) + + parameters.add_str( + display_name="Tip Rack", + variable_name="tiprack_load_name", + choices=[ + { + "display_name": "1000µl Filter Tip Rack", + "value": "opentrons_flex_96_filtertiprack_1000ul", + }, + { + "display_name": "1000µl Standard Tip Rack", + "value": "opentrons_flex_96_tiprack_1000ul", + }, + { + "display_name": "200µl Standard Tip Rack", + "value": "opentrons_flex_96_tiprack_200ul", + }, + { + "display_name": "200µl Filter Tip Rack", + "value": "opentrons_flex_96_filtertiprack_200ul", + }, + { + "display_name": "50µl Filter Tip Rack", + "value": "opentrons_flex_96_filtertiprack_50ul", + }, + { + "display_name": "50µl Standard Tip Rack", + "value": "opentrons_flex_96_tiprack_50ul", + }, + ], + default="opentrons_flex_96_tiprack_1000ul", + description="Select the tip rack type", + ) + + parameters.add_str( + display_name="Pipette Mount", + variable_name="pipette_mount", + choices=[ + {"display_name": "left", "value": "left"}, + {"display_name": "right", "value": "right"}, + ], + default="left", + description="Select the pipette mount.", + ) + + parameters.add_str( + display_name="Reservoir A", + variable_name="reservoir_a_load_name", + choices=reservoir_choices, + default="nest_1_reservoir_290ml", + description="Select the reservoir type", + ) + + parameters.add_str( + display_name="Reservoir B", + variable_name="reservoir_b_load_name", + choices=reservoir_choices, + default="nest_1_reservoir_290ml", + description="Select the reservoir type", + ) + + parameters.add_str( + display_name="Tiprack Position", + variable_name="tiprack_position", + default="B2", + description="Select the position of the tiprack", + choices=position_choices, + ) + + parameters.add_str( + display_name="Reservoir A Position", + variable_name="reservoir_a_position", + default="C1", + description="Select the position of reservoir A", + choices=position_choices, + ) + + parameters.add_str( + display_name="Reservoir B Position", + variable_name="reservoir_b_position", + default="D1", + description="Select the position of reservoir B", + choices=position_choices, + ) + + +####### END RTP DEFINITIONS ####### + + +def set_configure_nozzle_layout(ctx, pipette, tipracks, tip_config): + """Convenience function to set the nozzle layout of a pipette + with the given tip config we have mapped to a RTP.""" + ctx.comment(f"Setting nozzle layout for {pipette}") + ctx.comment(f"Tip config: {tip_config}") + if tip_config.api_end: + pipette.configure_nozzle_layout( + style=tip_config.api_tip_config, start=tip_config.api_start, end=tip_config.api_end, tip_racks=tipracks + ) + else: + pipette.configure_nozzle_layout(style=tip_config.api_tip_config, start=tip_config.api_start, tip_racks=tipracks) + + +def comment_tip_rack_status(ctx, tip_rack): + """ + Print out the tip status for each row in a tip rack. + Each row (A-H) will print the well statuses for columns 1-12 in a single comment, + with a '🟢' for present tips and a '❌' for missing tips. + """ + range_A_to_H = [chr(i) for i in range(ord("A"), ord("H") + 1)] + range_1_to_12 = range(1, 13) + + ctx.comment(f"Tip rack in {tip_rack.parent}") + + for row in range_A_to_H: + status_line = f"{row}: " + for col in range_1_to_12: + well = f"{row}{col}" + has_tip = tip_rack.wells_by_name()[well].has_tip + status_emoji = "🟢" if has_tip else "❌" + status_line += f"{well} {status_emoji} " + + # Print the full status line for the row + ctx.comment(status_line) + + +def run(ctx): + trash = ctx.load_trash_bin("A3") # must load trash bin + # get the key from the parameters + tip_config = find_partial_tip_config(ctx.params.partial_tip_config_key) + pipette_load_name = ctx.params.pipette_load_name + tiprack_load_name = ctx.params.tiprack_load_name + tip_rack_position = ctx.params.tiprack_position + pipette_mount = ctx.params.pipette_mount + reservoir_a_load_name = ctx.params.reservoir_a_load_name + reservoir_b_load_name = ctx.params.reservoir_b_load_name + reservoir_a_position = ctx.params.reservoir_a_position + reservoir_b_position = ctx.params.reservoir_b_position + # print out the tip config + ctx.comment(f"Running with {tip_config}") + ctx.comment(f"Using pipette {pipette_load_name}") + ctx.comment(f"Using tip rack {tiprack_load_name}") + ctx.comment(f"Using pipette mount {pipette_mount}") + ctx.comment(f"Using reservoir A {reservoir_a_load_name}") + ctx.comment(f"Using reservoir B {reservoir_b_load_name}") + ctx.comment(f"Using reservoir A position {reservoir_a_position}") + ctx.comment(f"Using reservoir B position {reservoir_b_position}") + # load the labware + reservoir_a = ctx.load_labware(reservoir_a_load_name, reservoir_a_position) + reservoir_b = ctx.load_labware(reservoir_b_load_name, reservoir_b_position) + # example code on Flex for a pipette + # comment shows we picked up the tips we expected + if tip_config.key == "no_tip_config" and pipette_load_name == "flex_96channel_1000": + tip_rack = ctx.load_labware(tiprack_load_name, tip_rack_position, adapter="opentrons_flex_96_tiprack_adapter") + else: + tip_rack = ctx.load_labware(tiprack_load_name, tip_rack_position) + pipette = ctx.load_instrument(pipette_load_name, pipette_mount) + # use this convenience function to set the nozzle layout + set_configure_nozzle_layout(ctx=ctx, pipette=pipette, tipracks=[tip_rack], tip_config=tip_config) + + def how_much_to_pipette(tiprack_load_name): + if "50" in tiprack_load_name: + return 20 + else: + return 100 + + volume = how_much_to_pipette(tiprack_load_name) + + pipette.pick_up_tip() + comment_tip_rack_status(ctx=ctx, tip_rack=tip_rack) + ctx.comment("aspirate from reservoir A") + pipette.aspirate(volume=volume, location=reservoir_a.wells()[0]) + ctx.comment("dispense to reservoir B") + pipette.dispense(volume=volume, location=reservoir_b.wells()[0]) + ctx.comment("mixing in reservoir B") + pipette.mix(repetitions=3, volume=volume / 2) + ctx.comment("Aspirate from reservoir A") + pipette.aspirate(volume=volume, location=reservoir_a.wells()[0]) + ctx.comment("Blow out in reservoir A") + pipette.blow_out() + ctx.comment("Aspirate from reservoir B") + pipette.aspirate(volume=volume, location=reservoir_b.wells()[0]) + ctx.comment("air_gap with no argument in reservoir B") + pipette.air_gap() + pipette.drop_tip() diff --git a/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[09676b9f7e][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_south_west].json 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When you're testing recovery, add tiprack back as necessary." + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "pickUpTip", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "748924f7bc1bc2293181fde240dd2497", + "notes": [], + "params": { + "labwareId": "UUID", + "pipetteId": "UUID", + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 178.38, + "y": 395.38, + "z": 99.0 + }, + "tipDiameter": 5.47, + "tipLength": 85.92999999999999, + "tipVolume": 1000.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "b9df600eccab4fa173cf714a9e113bac", + "notes": [], + "params": { + "message": "Overpressure - While Aspirating" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": 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+ "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "fa062920af0726db7ad4887c27b36880", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "0b302adfd08da748fe70219d2b2aec25", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "2eb6e6298939a64ca99771710a0f796c", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "09e6b07abc76b0f47eca5bde4a073fc8", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "f77abbc6fccb00d97c44105e4d0dc8c5", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "cf010ab2b5036733a0536650b665ef0d", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "7201cbd6da4b3a1f9f9a46b87e7f0406", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "2ede2f094fbeec343eed731eef8194e7", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "9d8af9e55efc49019e13e9deb41f8931", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "99f5375c033353b7601cae5b9aa65ac0", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "8c2e09cbe90d48fa924fb8cc51ab6f2e", + "notes": [], + "params": { + "message": "This tests Liquid Presence Detection - PLEASE MAKE SURE YOU'RE USING 1000UL TIPS OR YOU WILL BREAK THE PIPETTE" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "113ace5134dc05770bbbc9f25616e4d2", + "notes": [], + "params": { + "message": "Make sure reservoir is empty, and have full reservoir on standby to switch out with for recovery" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "liquidProbe", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "1c5720bacb264e3da2b314e18b3591c5", + "notes": [], + "params": { + "labwareId": "UUID", + "pipetteId": "UUID", + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 2.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 342.38, + "y": 256.78, + "z": 33.4 + }, + "z_position": 0.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "e0bbd11eb919a3804d1d23771f645a8d", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": -25.85 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 342.38, + "y": 256.78, + "z": 5.55 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "b66c39dd7f7222e62b5c25ef63dc4eaa", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": -25.85 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 342.38, + "y": 256.78, + "z": 5.55 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "1f47f37d8e4b3aea8bbea66e7014ab27", + "notes": [], + "params": { + "axes": [ + "leftPlunger", + "leftZ" + ] + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "0f904e31fcd8dcc7ac6778e30cb61bb8", + "notes": [], + "params": { + "message": "Overpressure - While Dispensing" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "f04ecdcd9f9b5219d1cf73c37e540d9f", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 19.999999999999993 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 64.39999999999999 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "690df57327aeb3c16ca4ad85203d1ec5", + "notes": [], + "params": { + "message": "Swap with tip(s) that are hot glued shut (you will need to cut the tips for retry recovery options)" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "631e9e0a8230489d6abb8665f0f13463", + "notes": [], + "params": { + "flowRate": 716.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 900.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 19.999999999999993 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 64.39999999999999 + }, + "volume": 900.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "moveToAddressableAreaForDropTip", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "0e801e389ac14f125f5ef5db2eae3d08", + "notes": [], + "params": { + "addressableAreaName": "movableTrashA3", + "alternateDropLocation": false, + "forceDirect": false, + "ignoreTipConfiguration": true, + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "pipetteId": "UUID" + }, + "result": { + "position": { + "x": 434.25, + "y": 364.0, + "z": 40.0 + } + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "20b6b0a1dd75709308db2b292ec03992", + "notes": [], + "params": { + "message": "You will have to manually remove tips if you're using a modified pipette" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "moveToAddressableAreaForDropTip", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "d7fecbae31209f6f04a2ee350e584c46", + "notes": [], + "params": { + "addressableAreaName": "movableTrashA3", + "alternateDropLocation": false, + "forceDirect": false, + "ignoreTipConfiguration": true, + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "pipetteId": "UUID" + }, + "result": { + "position": { + "x": 434.25, + "y": 364.0, + "z": 40.0 + } + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dropTipInPlace", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "c4e9dc65f4db718d38cf627ad786ac83", + "notes": [], + "params": { + "pipetteId": "UUID" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "65bf172b33169e051174a2d2c6ff08f9", + "notes": [], + "params": { + "message": "This tests Tip Pick Up Failure (General Error for now)" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "eddba592c53196f06dd465abd040ab57", + "notes": [], + "params": { + "message": "Please remove tip rack from deck. When you're testing recovery, add tiprack back as necessary." + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "pickUpTip", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "d3fb46eb1e9183a7897d150cc1ef5898", + "notes": [], + "params": { + "labwareId": "UUID", + "pipetteId": "UUID", + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 178.38, + "y": 288.38, + "z": 99.0 + }, + "tipDiameter": 5.58, + "tipLength": 47.81, + "tipVolume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "5019b8a5d55914c4a86174b01c40d297", + "notes": [], + "params": { + "message": "Overpressure - While Aspirating" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "7aafe07a40a3c427dee717cc84c6e303", + "notes": [], + "params": { + "axes": [ + "rightPlunger", + "rightZ" + ] + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "2630be2e9b808711cf5ff3c5d69815df", + "notes": [], + "params": { + "message": "Use p50UL tips - Swap with tip(s) that are hot glued shut (you will need to swap the tips for retry recovery options)" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "2492d664f9c55efed5652b1cbcf9fdde", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "b2d6eb28bc88f476044b1e7e4d6b4107", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "737fbfd381fa1576044bb4666a4c9483", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "e45f68ed478fc9f9825f00ff31fcde46", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "9dead7e7447f7a0dbdb9b2145d28c692", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "f154f5a1679179fabd7a55e8e26d0992", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "e20d2093cb882e562bdf5bc427646443", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "b0a14d30a958196db673fa5ac8eae550", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "dcba87cf3be1f8600a2dea25b0a83274", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "c9c41ee06959a54f1aab7a8c5119912b", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "e4c179da374f4f5ba047d341a2aa629e", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "bd6698a069e856a66de54a83992d695f", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "6d4f1b88b6955d6574e48e31084aeac4", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "147d5fc4adfbe0a46301abaa465df262", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "b660287ab347393357863827d926402d", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "afaecd2d3f12f463dc0c1596fbec73d8", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "18a206fcf40b85ef77d65a95eb69b977", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "a66f9c581041537d7c9a0b5b83b2a3bb", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "ff3ce00c290b4f3a025b51cc08e87e7f", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "0c2b7f9b5117e811de2ca8c708719a4f", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 44.4 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "16b3eb79760f3264be5d1d29a9405940", + "notes": [], + "params": { + "message": "This tests Liquid Presence Detection - PLEASE MAKE SURE YOU'RE USING 1000UL TIPS OR YOU WILL BREAK THE PIPETTE" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "448d8d14a4ba598c39cedae64f1a7d1d", + "notes": [], + "params": { + "message": "Make sure reservoir is empty, and have full reservoir on standby to switch out with for recovery" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "liquidProbe", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "de2087c49b77fe03a83649111113c157", + "notes": [], + "params": { + "labwareId": "UUID", + "pipetteId": "UUID", + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 2.0 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 342.38, + "y": 256.78, + "z": 33.4 + }, + "z_position": 0.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "bcb050e4952ef2d7f7c42dd6838c3c24", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": -25.85 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 342.38, + "y": 256.78, + "z": 5.55 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "48dbf180376bece74dd403b1407f9db6", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": -25.85 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 342.38, + "y": 256.78, + "z": 5.55 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "431a83945a647ae1699424f1b19555d4", + "notes": [], + "params": { + "axes": [ + "rightPlunger", + "rightZ" + ] + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "d291fec87c615fe4446dd14989ec6503", + "notes": [], + "params": { + "message": "Overpressure - While Dispensing" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "aspirate", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "29849adcc3a3cc1c478077d9336f1037", + "notes": [], + "params": { + "flowRate": 35.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 19.999999999999993 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 64.39999999999999 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "5a2ff23afb6c9e0d8d201e674d3ce9f3", + "notes": [], + "params": { + "message": "Swap with tip(s) that are hot glued shut (you will need to cut the tips for retry recovery options)" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dispense", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "25dff8ae10364bc6e427439df62fd75e", + "notes": [], + "params": { + "flowRate": 57.0, + "labwareId": "UUID", + "pipetteId": "UUID", + "volume": 50.0, + "wellLocation": { + "offset": { + "x": 0.0, + "y": 0.0, + "z": 19.999999999999993 + }, + "origin": "top" + }, + "wellName": "A1" + }, + "result": { + "position": { + "x": 391.88, + "y": 42.74, + "z": 64.39999999999999 + }, + "volume": 50.0 + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "moveToAddressableAreaForDropTip", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "a3cd8b7881d952254804421be5e8229e", + "notes": [], + "params": { + "addressableAreaName": "movableTrashA3", + "alternateDropLocation": false, + "forceDirect": false, + "ignoreTipConfiguration": true, + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "pipetteId": "UUID" + }, + "result": { + "position": { + "x": 434.25, + "y": 364.0, + "z": 40.0 + } + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "waitForResume", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "96228aa179a3137a865a4889db38242d", + "notes": [], + "params": { + "message": "You will have to manually remove tips if you're using a modified pipette" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "moveToAddressableAreaForDropTip", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "c5ad2c9360fc17ec095d7bdc27b726d4", + "notes": [], + "params": { + "addressableAreaName": "movableTrashA3", + "alternateDropLocation": false, + "forceDirect": false, + "ignoreTipConfiguration": true, + "offset": { + "x": 0.0, + "y": 0.0, + "z": 0.0 + }, + "pipetteId": "UUID" + }, + "result": { + "position": { + "x": 434.25, + "y": 364.0, + "z": 40.0 + } + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "dropTipInPlace", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "f32667674c9e53f66e65233250b4e939", + "notes": [], + "params": { + "pipetteId": "UUID" + }, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + } + ], + "config": { + "apiVersion": [ + 2, + 20 + ], + "protocolType": "python" + }, + "createdAt": "TIMESTAMP", + "errors": [], + "files": [ + { + "name": "Flex_S_v2_20_P8X1000_P50_LLD.py", + "role": "main" + } + ], + "labware": [ + { + "definitionUri": "opentrons/opentrons_flex_96_tiprack_1000ul/1", + "id": "UUID", + "loadName": "opentrons_flex_96_tiprack_1000ul", + "location": { + "slotName": "A2" + } + }, + { + "definitionUri": "opentrons/opentrons_flex_96_tiprack_50ul/1", + "id": "UUID", + "loadName": "opentrons_flex_96_tiprack_50ul", + "location": { + "slotName": "B2" + } + }, + { + "definitionUri": "opentrons/armadillo_96_wellplate_200ul_pcr_full_skirt/2", + "id": "UUID", + "loadName": "armadillo_96_wellplate_200ul_pcr_full_skirt", + "location": { + "slotName": "C3" + } + }, + { + "definitionUri": "opentrons/nest_1_reservoir_290ml/1", + "id": "UUID", + "loadName": "nest_1_reservoir_290ml", + "location": { + "slotName": "D3" + } + }, + { + "definitionUri": "opentrons/nest_12_reservoir_15ml/1", + "id": "UUID", + "loadName": "nest_12_reservoir_15ml", + "location": { + "slotName": "B3" + } + } + ], + "liquids": [ + { + "description": "water for ER testing", + "displayColor": "#90e0ef", + "displayName": "Water", + "id": "UUID" + }, + { + "description": "Water for ER testing", + "displayColor": "#0077b6", + "displayName": "Water but more blue", + "id": "UUID" + } + ], + "metadata": { + "author": "Sara Kowalski", + "description": "Simple Protocol that user can use to Phase 1 Error Recovery options for single and multi channel pipettes. NOTE: YOU WILL NEED A MODIFIED PIPETTE (NO SHROUD), and changed order of operations to reduce impact of dispense ER failure", + "protocolName": "2.20 Error Recovery Testing Protocol - Modified" + }, + "modules": [], + "pipettes": [ + { + "id": "UUID", + "mount": "left", + "pipetteName": "p1000_multi_flex" + }, + { + "id": "UUID", + "mount": "right", + "pipetteName": "p50_single_flex" + } + ], + "result": "ok", + "robotType": "OT-3 Standard", + "runTimeParameters": [] +} diff --git a/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[196ec488f7][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_west].json b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[196ec488f7][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_west].json new file mode 100644 index 00000000000..98709caf300 --- /dev/null +++ b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[196ec488f7][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_west].json @@ -0,0 +1,1500 @@ +{ + "commands": [ + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "50c7ae73a4e3f7129874f39dfb514803", + "notes": [], + "params": {}, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "loadLabware", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "73d9d4d55ae8466f3a793ceb70545fa5", + "notes": [], + "params": { + "loadName": "opentrons_96_tiprack_1000ul", + "location": { + "slotName": "C2" + }, + "namespace": "opentrons", + "version": 1 + }, + "result": { + "definition": { + "allowedRoles": [], + "brand": { + "brand": "Opentrons", + "brandId": [], + "links": [ + "https://shop.opentrons.com/collections/opentrons-tips/products/opentrons-1000ul-tips" + ] + }, + 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Partial column configurations require the 'end' parameter.", + "errorCode": "4000", + "errorInfo": {}, + "errorType": "ExceptionInProtocolError", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "ValueError: Partial column configurations require the 'end' parameter.", + "errorCode": "4000", + "errorInfo": { + "args": "(\"Partial column configurations require the 'end' parameter.\",)", + "class": "ValueError", + "traceback": " File \"/usr/local/lib/python3.10/site-packages/opentrons/protocols/execution/execute_python.py\", line N, in exec_run\n exec(\"run(__context)\", new_globs)\n\n File \"\", line N, in \n\n File \"Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_eight_partial_column_no_end.py\", line N, in run\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocols/api_support/util.py\", line N, in _check_version_wrapper\n return decorated_obj(*args, **kwargs)\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocol_api/instrument_context.py\", line N, in configure_nozzle_layout\n validated_end = _check_valid_end_nozzle(validated_start, end)\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocol_api/instrument_context.py\", line N, in _check_valid_end_nozzle\n raise ValueError(\"Partial column configurations require the 'end' parameter.\")\n" + }, + "errorType": "PythonException", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [] + } + ] + } + ], + "files": [ + { + "name": "Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_eight_partial_column_no_end.py", + "role": "main" + } + ], + "labware": [ + { + "definitionUri": "opentrons/opentrons_flex_96_tiprack_1000ul/1", + "id": "UUID", + "loadName": "opentrons_flex_96_tiprack_1000ul", + "location": { + "slotName": "B2" + } + } + ], + "liquids": [], + "metadata": { + "description": "oooo", + "protocolName": "Invalid tip configs that should error" + }, + "modules": [], + "pipettes": [ + { + "id": "UUID", + "mount": "left", + "pipetteName": "p1000_multi_flex" + } + ], + "result": "not-ok", + "robotType": "OT-3 Standard", + "runTimeParameters": [] +} diff --git a/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[53db9bf516][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_eight_partial_column_bottom_left].json b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[53db9bf516][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_eight_partial_column_bottom_left].json new file mode 100644 index 00000000000..662d6cf0c4b --- /dev/null +++ b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[53db9bf516][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_eight_partial_column_bottom_left].json @@ -0,0 +1,1296 @@ +{ + "commands": [ + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": 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"pipetteId": "UUID" + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + } + ], + "config": { + "apiVersion": [ + 2, + 20 + ], + "protocolType": "python" + }, + "createdAt": "TIMESTAMP", + "errors": [ + { + "createdAt": "TIMESTAMP", + "detail": "ValueError [line 134]: Partial column configurations require the 'end' parameter.", + "errorCode": "4000", + "errorInfo": {}, + "errorType": "ExceptionInProtocolError", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "ValueError: Partial column configurations require the 'end' parameter.", + "errorCode": "4000", + "errorInfo": { + "args": "(\"Partial column configurations require the 'end' parameter.\",)", + "class": "ValueError", + "traceback": " File \"/usr/local/lib/python3.10/site-packages/opentrons/protocols/execution/execute_python.py\", line N, in exec_run\n exec(\"run(__context)\", new_globs)\n\n File \"\", line N, in \n\n File \"OT2_X_v2_20_8_Overrides_InvalidConfigs_Override_eight_partial_column_no_end.py\", line N, in run\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocols/api_support/util.py\", line N, in _check_version_wrapper\n return decorated_obj(*args, **kwargs)\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocol_api/instrument_context.py\", line N, in configure_nozzle_layout\n validated_end = _check_valid_end_nozzle(validated_start, end)\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocol_api/instrument_context.py\", line N, in _check_valid_end_nozzle\n raise ValueError(\"Partial column configurations require the 'end' parameter.\")\n" + }, + "errorType": "PythonException", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [] + } + ] + } + ], + "files": [ + { + "name": "OT2_X_v2_20_8_Overrides_InvalidConfigs_Override_eight_partial_column_no_end.py", + "role": "main" + } + ], + "labware": [ + { + "definitionUri": 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b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[5edb9b4de3][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_2].json new file mode 100644 index 00000000000..a107fa87e60 --- /dev/null +++ b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[5edb9b4de3][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_2].json @@ -0,0 +1,1260 @@ +{ + "commands": [ + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "50c7ae73a4e3f7129874f39dfb514803", + "notes": [], + "params": {}, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "loadLabware", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "73d9d4d55ae8466f3a793ceb70545fa5", + "notes": [], + "params": { + "loadName": "opentrons_flex_96_tiprack_1000ul", + "location": 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"pipetteId": "UUID" + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + } + ], + "config": { + "apiVersion": [ + 2, + 20 + ], + "protocolType": "python" + }, + "createdAt": "TIMESTAMP", + "errors": [ + { + "createdAt": "TIMESTAMP", + "detail": "ValueError [line 167]: Partial column configuration is only supported on 8-Channel pipettes.", + "errorCode": "4000", + "errorInfo": {}, + "errorType": "ExceptionInProtocolError", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "ValueError: Partial column configuration is only supported on 8-Channel pipettes.", + "errorCode": "4000", + "errorInfo": { + "args": "('Partial column configuration is only supported on 8-Channel pipettes.',)", + "class": "ValueError", + "traceback": " File \"/usr/local/lib/python3.10/site-packages/opentrons/protocols/execution/execute_python.py\", line N, in exec_run\n exec(\"run(__context)\", new_globs)\n\n File \"\", line N, in \n\n File \"Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_2.py\", line N, in run\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocols/api_support/util.py\", line N, in _check_version_wrapper\n return decorated_obj(*args, **kwargs)\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocol_api/instrument_context.py\", line N, in configure_nozzle_layout\n self._raise_if_configuration_not_supported_by_pipette(style)\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocol_api/instrument_context.py\", line N, in _raise_if_configuration_not_supported_by_pipette\n raise ValueError(\n" + }, + "errorType": "PythonException", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [] + } + ] + } + ], + "files": [ + { + "name": "Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_2.py", + "role": "main" + } + ], + "labware": [ + { + "definitionUri": "opentrons/opentrons_flex_96_tiprack_1000ul/1", + "id": "UUID", + "loadName": "opentrons_flex_96_tiprack_1000ul", + "location": { + "slotName": "B2" + } + } + ], + "liquids": [], + "metadata": { + "description": "oooo", + "protocolName": "Invalid tip configs that should error" + }, + "modules": [], + "pipettes": [ + { + "id": "UUID", + "mount": "left", + "pipetteName": "p1000_96" + } + ], + "result": "not-ok", + "robotType": "OT-3 Standard", + "runTimeParameters": [] +} diff --git a/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[6122c72996][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_1].json b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[6122c72996][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_1].json new file mode 100644 index 00000000000..1dcac6e453a --- /dev/null +++ b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[6122c72996][Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_1].json @@ -0,0 +1,1260 @@ +{ + "commands": [ + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "50c7ae73a4e3f7129874f39dfb514803", + "notes": [], + "params": {}, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "loadLabware", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "73d9d4d55ae8466f3a793ceb70545fa5", + "notes": [], + "params": { + "loadName": "opentrons_flex_96_tiprack_1000ul", + "location": { + "slotName": "B2" + }, + "namespace": "opentrons", + "version": 1 + }, + "result": { + "definition": { + "allowedRoles": [], + "brand": { + "brand": "Opentrons", + "brandId": [] + }, + "cornerOffsetFromSlot": { + "x": 0, + "y": 0, + "z": 0 + }, + "dimensions": { + "xDimension": 127.75, + "yDimension": 85.75, + "zDimension": 99 + }, + "gripForce": 16.0, + "gripHeightFromLabwareBottom": 23.9, + "gripperOffsets": {}, + "groups": [ + { + "metadata": {}, + "wells": [ + "A1", + "A10", + "A11", + "A12", + "A2", + "A3", + "A4", + "A5", + "A6", + "A7", + "A8", + "A9", + "B1", + "B10", + "B11", + "B12", + "B2", + "B3", + "B4", + "B5", + "B6", + "B7", + "B8", + "B9", + "C1", + "C10", + "C11", + "C12", + "C2", + "C3", + "C4", + "C5", + "C6", + "C7", + "C8", + "C9", + "D1", + "D10", + "D11", + "D12", + "D2", + "D3", + "D4", + "D5", + "D6", + "D7", + "D8", + "D9", + "E1", + "E10", + "E11", + "E12", + "E2", + "E3", + "E4", + "E5", + "E6", + "E7", + "E8", + "E9", + "F1", + "F10", + "F11", + "F12", + "F2", + "F3", + "F4", + "F5", + "F6", + "F7", + "F8", + "F9", + "G1", + "G10", + "G11", + "G12", + "G2", + "G3", + "G4", + "G5", + "G6", + "G7", + "G8", + "G9", + "H1", + "H10", + "H11", + "H12", + "H2", + "H3", + "H4", + "H5", + "H6", + "H7", + "H8", + "H9" + ] + } + ], + "metadata": { + "displayCategory": "tipRack", + "displayName": "Opentrons Flex 96 Tip Rack 1000 µL", + "displayVolumeUnits": "µL", + "tags": [] + }, + "namespace": "opentrons", + "ordering": [ + [ + "A1", + "B1", + "C1", + "D1", + "E1", + "F1", + "G1", + "H1" + ], + [ + "A10", + "B10", + "C10", + "D10", + "E10", + "F10", + "G10", + "H10" + ], + [ + "A11", + "B11", + "C11", + "D11", + "E11", + "F11", + "G11", + "H11" + ], + [ + "A12", + "B12", + "C12", + "D12", + "E12", + "F12", + "G12", + "H12" + ], + [ + "A2", + "B2", + "C2", + "D2", + "E2", + "F2", + "G2", + "H2" + ], + [ + "A3", + "B3", + "C3", + "D3", + "E3", + "F3", + "G3", + "H3" + ], + [ + "A4", + "B4", + "C4", + "D4", + "E4", + "F4", + "G4", + "H4" + ], + [ + "A5", + "B5", + "C5", + "D5", + "E5", + "F5", + "G5", + "H5" + ], + [ + "A6", + "B6", + "C6", + "D6", + "E6", + "F6", + "G6", + "H6" + ], + [ + "A7", + "B7", + "C7", + "D7", + "E7", + "F7", + "G7", + "H7" + ], + [ + "A8", + "B8", + "C8", + "D8", + "E8", + "F8", + 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column configuration is only supported on 8-Channel pipettes.", + "errorCode": "4000", + "errorInfo": {}, + "errorType": "ExceptionInProtocolError", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "ValueError: Partial column configuration is only supported on 8-Channel pipettes.", + "errorCode": "4000", + "errorInfo": { + "args": "('Partial column configuration is only supported on 8-Channel pipettes.',)", + "class": "ValueError", + "traceback": " File \"/usr/local/lib/python3.10/site-packages/opentrons/protocols/execution/execute_python.py\", line N, in exec_run\n exec(\"run(__context)\", new_globs)\n\n File \"\", line N, in \n\n File \"Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_ninety_six_partial_column_1.py\", line N, in run\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/protocols/api_support/util.py\", line N, in _check_version_wrapper\n return decorated_obj(*args, **kwargs)\n\n File 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"UUID", + "mount": "left", + "pipetteName": "p1000_96" + } + ], + "result": "not-ok", + "robotType": "OT-3 Standard", + "runTimeParameters": [] +} diff --git a/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[675d2c2562][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_south_east].json b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[675d2c2562][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_south_east].json new file mode 100644 index 00000000000..1bf35620512 --- /dev/null +++ b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[675d2c2562][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_south_east].json @@ -0,0 +1,1500 @@ +{ + "commands": [ + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "50c7ae73a4e3f7129874f39dfb514803", + "notes": [], + "params": {}, + "result": {}, + 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"totalLiquidVolume": 300, + "x": 113.38, + "y": 38.24, + "z": 5.39 + }, + "E2": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 23.38, + "y": 38.24, + "z": 5.39 + }, + "E3": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 32.38, + "y": 38.24, + "z": 5.39 + }, + "E4": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 41.38, + "y": 38.24, + "z": 5.39 + }, + "E5": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 50.38, + "y": 38.24, + "z": 5.39 + }, + "E6": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 59.38, + "y": 38.24, + "z": 5.39 + }, + "E7": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 68.38, + "y": 38.24, + "z": 5.39 + }, + "E8": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 77.38, + "y": 38.24, + "z": 5.39 + }, + "E9": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 86.38, + "y": 38.24, + "z": 5.39 + }, + "F1": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 14.38, + "y": 29.24, + "z": 5.39 + }, + "F10": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 95.38, + "y": 29.24, + "z": 5.39 + }, + "F11": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 104.38, + "y": 29.24, + "z": 5.39 + }, + "F12": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 113.38, + "y": 29.24, + "z": 5.39 + }, + "F2": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 23.38, + "y": 29.24, + "z": 5.39 + }, + "F3": { + "depth": 59.3, + "diameter": 5.23, + "shape": 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"circular", + "totalLiquidVolume": 300, + "x": 14.38, + "y": 20.24, + "z": 5.39 + }, + "G10": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 95.38, + "y": 20.24, + "z": 5.39 + }, + "G11": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 104.38, + "y": 20.24, + "z": 5.39 + }, + "G12": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 113.38, + "y": 20.24, + "z": 5.39 + }, + "G2": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 23.38, + "y": 20.24, + "z": 5.39 + }, + "G3": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 32.38, + "y": 20.24, + "z": 5.39 + }, + "G4": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 41.38, + "y": 20.24, + "z": 5.39 + }, + "G5": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 50.38, + "y": 20.24, + "z": 5.39 + }, + "G6": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 59.38, + "y": 20.24, + "z": 5.39 + }, + "G7": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 68.38, + "y": 20.24, + "z": 5.39 + }, + "G8": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 77.38, + "y": 20.24, + "z": 5.39 + }, + "G9": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 86.38, + "y": 20.24, + "z": 5.39 + }, + "H1": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 14.38, + "y": 11.24, + "z": 5.39 + }, + "H10": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 95.38, + "y": 11.24, + "z": 5.39 + }, + "H11": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 104.38, + "y": 11.24, + "z": 5.39 + }, + "H12": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 113.38, + "y": 11.24, + "z": 5.39 + }, + "H2": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 23.38, + "y": 11.24, + "z": 5.39 + }, + "H3": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 32.38, + "y": 11.24, + "z": 5.39 + }, + "H4": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 41.38, + "y": 11.24, + "z": 5.39 + }, + "H5": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 50.38, + "y": 11.24, + "z": 5.39 + }, + "H6": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 59.38, + "y": 11.24, + "z": 5.39 + }, + "H7": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 68.38, + "y": 11.24, + "z": 5.39 + }, + "H8": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 77.38, + "y": 11.24, + "z": 5.39 + }, + "H9": { + "depth": 59.3, + "diameter": 5.23, + "shape": "circular", + "totalLiquidVolume": 300, + "x": 86.38, + "y": 11.24, + "z": 5.39 + } + } + }, + "labwareId": "UUID" + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "loadPipette", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "54fddab1eb921f6e1d3aa7e96d2ac307", + "notes": [], + "params": { + "liquidPresenceDetection": false, + "mount": "left", + "pipetteName": "p20_multi_gen2", + "tipOverlapNotAfterVersion": "v3" + }, + "result": { + "pipetteId": "UUID" + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "configureNozzleLayout", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "error": { + "createdAt": "TIMESTAMP", + "detail": "No entry for back left nozzle 'G12' in pipette", + "errorCode": "4007", + "errorInfo": {}, + "errorType": "IncompatibleNozzleConfiguration", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "KeyError: 'G12'", + "errorCode": "4000", + "errorInfo": { + "args": "('G12',)", + "class": "KeyError", + "traceback": " File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in build\n back_left_row_index, back_left_column_index = _row_col_indices_for_nozzle(\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in _row_col_indices_for_nozzle\n return _row_or_col_index_for_nozzle(rows, nozzle), _row_or_col_index_for_nozzle(\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in _row_or_col_index_for_nozzle\n raise KeyError(nozzle)\n" + }, + "errorType": "PythonException", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [] + } + ] + }, + "id": "UUID", + "key": "4b1d27a6f17f312dd76668f0c48ed406", + "notes": [], + "params": { + "configurationParams": { + "backLeftNozzle": "G12", + "frontRightNozzle": "H1", + "primaryNozzle": "H1", + "style": "QUADRANT" + }, + "pipetteId": "UUID" + }, + "startedAt": "TIMESTAMP", + "status": "failed" + } + ], + "config": { + "apiVersion": [ + 2, + 20 + ], + "protocolType": "python" + }, + "createdAt": "TIMESTAMP", + "errors": [ + { + "createdAt": "TIMESTAMP", + "detail": "ProtocolCommandFailedError [line 134]: Error 4000 GENERAL_ERROR (ProtocolCommandFailedError): IncompatibleNozzleConfiguration: No entry for back left nozzle 'G12' in pipette", + "errorCode": "4000", + "errorInfo": {}, + "errorType": "ExceptionInProtocolError", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "IncompatibleNozzleConfiguration: No entry for back left nozzle 'G12' in pipette", + "errorCode": "4000", + "errorInfo": {}, + "errorType": "ProtocolCommandFailedError", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "No entry for back left nozzle 'G12' in pipette", + "errorCode": "4007", + "errorInfo": {}, + "errorType": "IncompatibleNozzleConfiguration", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "KeyError: 'G12'", + "errorCode": "4000", + "errorInfo": { + "args": "('G12',)", + "class": "KeyError", + "traceback": " File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in build\n back_left_row_index, back_left_column_index = _row_col_indices_for_nozzle(\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in _row_col_indices_for_nozzle\n return _row_or_col_index_for_nozzle(rows, nozzle), _row_or_col_index_for_nozzle(\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in _row_or_col_index_for_nozzle\n raise KeyError(nozzle)\n" + }, + "errorType": "PythonException", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [] + } + ] + } + ] + } + ] + } + ], + "files": [ + { + "name": "OT2_X_v2_20_8_Overrides_InvalidConfigs_Override_eight_partial_column_bottom_right.py", + "role": "main" + } + ], + "labware": [ + { + "definitionUri": "opentrons/opentrons_96_tiprack_20ul/1", + "id": "UUID", + "loadName": "opentrons_96_tiprack_20ul", + "location": { + "slotName": "1" + } + }, + { + "definitionUri": "opentrons/opentrons_96_tiprack_300ul/1", + "id": "UUID", + "loadName": "opentrons_96_tiprack_300ul", + "location": { + "slotName": "2" + } + } + ], + "liquids": [], + "metadata": { + "description": "oooo", + "protocolName": "Invalid tip configs that should error" + }, + "modules": [], + "pipettes": [ + { + "id": "UUID", + "mount": "left", + "pipetteName": "p20_multi_gen2" + } + ], + "result": "not-ok", + "robotType": "OT-2 Standard", + "runTimeParameters": [] +} diff --git a/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[a0b755a1a1][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_north_west].json b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[a0b755a1a1][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_north_west].json new file mode 100644 index 00000000000..eca34fc28c3 --- /dev/null +++ b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[a0b755a1a1][Flex_X_v2_20_96_None_Overrides_TooTallLabware_Override_north_west].json @@ -0,0 +1,1500 @@ +{ + "commands": [ + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "50c7ae73a4e3f7129874f39dfb514803", + "notes": [], + "params": {}, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "loadLabware", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "73d9d4d55ae8466f3a793ceb70545fa5", + "notes": [], + "params": { + "loadName": "opentrons_96_tiprack_1000ul", + "location": { + "slotName": "C2" + }, + "namespace": "opentrons", + "version": 1 + }, + "result": { + "definition": { + "allowedRoles": [], + "brand": { + "brand": "Opentrons", + "brandId": [], + "links": [ + "https://shop.opentrons.com/collections/opentrons-tips/products/opentrons-1000ul-tips" + ] + }, + "cornerOffsetFromSlot": { + "x": 0, + "y": 0, + "z": 0 + }, + "dimensions": { + "xDimension": 127.76, + "yDimension": 85.48, + "zDimension": 97.47 + }, + "gripperOffsets": {}, + "groups": [ + { + "metadata": {}, + "wells": [ + "A1", + "A10", + "A11", + "A12", + "A2", + "A3", + "A4", + "A5", + "A6", + "A7", + "A8", + "A9", + "B1", + "B10", + "B11", + "B12", + "B2", + "B3", + "B4", + "B5", + "B6", + "B7", + "B8", + "B9", + "C1", + "C10", + 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}, + "E2": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 23.38, + "y": 38.38, + "z": 1.5 + }, + "E3": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 32.38, + "y": 38.38, + "z": 1.5 + }, + "E4": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 41.38, + "y": 38.38, + "z": 1.5 + }, + "E5": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 50.38, + "y": 38.38, + "z": 1.5 + }, + "E6": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 59.38, + "y": 38.38, + "z": 1.5 + }, + "E7": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 68.38, + "y": 38.38, + "z": 1.5 + }, + "E8": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 77.38, + "y": 38.38, + "z": 1.5 + }, + "E9": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 86.38, + "y": 38.38, + "z": 1.5 + }, + "F1": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 14.38, + "y": 29.38, + "z": 1.5 + }, + "F10": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 95.38, + "y": 29.38, + "z": 1.5 + }, + "F11": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 104.38, + "y": 29.38, + "z": 1.5 + }, + "F12": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 113.38, + "y": 29.38, + "z": 1.5 + }, + "F2": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 23.38, + "y": 29.38, + "z": 1.5 + }, + "F3": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 32.38, + "y": 29.38, + "z": 1.5 + }, + "F4": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 41.38, + "y": 29.38, + "z": 1.5 + }, + "F5": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 50.38, + "y": 29.38, + "z": 1.5 + }, + "F6": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 59.38, + "y": 29.38, + "z": 1.5 + }, + "F7": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 68.38, + "y": 29.38, + "z": 1.5 + }, + "F8": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 77.38, + "y": 29.38, + "z": 1.5 + }, + "F9": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 86.38, + "y": 29.38, + "z": 1.5 + }, + "G1": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 14.38, + "y": 20.38, + "z": 1.5 + }, + "G10": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 95.38, + "y": 20.38, + "z": 1.5 + }, + "G11": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 104.38, + "y": 20.38, + "z": 1.5 + }, + "G12": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 113.38, + "y": 20.38, + "z": 1.5 + }, + "G2": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 23.38, + "y": 20.38, + "z": 1.5 + }, + "G3": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 32.38, + "y": 20.38, + "z": 1.5 + }, + "G4": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 41.38, + "y": 20.38, + "z": 1.5 + }, + "G5": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 50.38, + "y": 20.38, + "z": 1.5 + }, + "G6": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 59.38, + "y": 20.38, + "z": 1.5 + }, + "G7": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 68.38, + "y": 20.38, + "z": 1.5 + }, + "G8": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 77.38, + "y": 20.38, + "z": 1.5 + }, + "G9": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 86.38, + "y": 20.38, + "z": 1.5 + }, + "H1": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 14.38, + "y": 11.38, + "z": 1.5 + }, + "H10": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 95.38, + "y": 11.38, + "z": 1.5 + }, + "H11": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 104.38, + "y": 11.38, + "z": 1.5 + }, + "H12": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 113.38, + "y": 11.38, + "z": 1.5 + }, + "H2": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 23.38, + "y": 11.38, + "z": 1.5 + }, + "H3": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 32.38, + "y": 11.38, + "z": 1.5 + }, + "H4": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 41.38, + "y": 11.38, + "z": 1.5 + }, + "H5": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 50.38, + "y": 11.38, + "z": 1.5 + }, + "H6": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 59.38, + "y": 11.38, + "z": 1.5 + }, + "H7": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 68.38, + "y": 11.38, + "z": 1.5 + }, + "H8": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 77.38, + "y": 11.38, + "z": 1.5 + }, + "H9": { + "depth": 97.5, + "diameter": 5.47, + "shape": "circular", + "totalLiquidVolume": 1000, + "x": 86.38, + "y": 11.38, + "z": 1.5 + } + } + }, + "labwareId": "UUID" + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "loadPipette", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "bd403a1c851a75b4b68ce34796d713fa", + "notes": [], + "params": { + "liquidPresenceDetection": false, + "mount": "left", + "pipetteName": "p1000_multi_flex", + "tipOverlapNotAfterVersion": "v3" + }, + "result": { + "pipetteId": "UUID" + }, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "configureNozzleLayout", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "error": { + "createdAt": "TIMESTAMP", + "detail": "No entry for back left nozzle 'G12' in pipette", + "errorCode": "4007", + "errorInfo": {}, + "errorType": "IncompatibleNozzleConfiguration", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "KeyError: 'G12'", + "errorCode": "4000", + "errorInfo": { + "args": "('G12',)", + "class": "KeyError", + "traceback": " File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in build\n back_left_row_index, back_left_column_index = _row_col_indices_for_nozzle(\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in _row_col_indices_for_nozzle\n return _row_or_col_index_for_nozzle(rows, nozzle), _row_or_col_index_for_nozzle(\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in _row_or_col_index_for_nozzle\n raise KeyError(nozzle)\n" + }, + "errorType": "PythonException", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [] + } + ] + }, + "id": "UUID", + "key": "2c37ad797da7df791b57a7843a203e88", + "notes": [], + "params": { + "configurationParams": { + "backLeftNozzle": "G12", + "frontRightNozzle": "H1", + "primaryNozzle": "H1", + "style": "QUADRANT" + }, + "pipetteId": "UUID" + }, + "startedAt": "TIMESTAMP", + "status": "failed" + } + ], + "config": { + "apiVersion": [ + 2, + 20 + ], + "protocolType": "python" + }, + "createdAt": "TIMESTAMP", + "errors": [ + { + "createdAt": "TIMESTAMP", + "detail": "ProtocolCommandFailedError [line 167]: Error 4000 GENERAL_ERROR (ProtocolCommandFailedError): IncompatibleNozzleConfiguration: No entry for back left nozzle 'G12' in pipette", + "errorCode": "4000", + "errorInfo": {}, + "errorType": "ExceptionInProtocolError", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "IncompatibleNozzleConfiguration: No entry for back left nozzle 'G12' in pipette", + "errorCode": "4000", + "errorInfo": {}, + "errorType": "ProtocolCommandFailedError", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "No entry for back left nozzle 'G12' in pipette", + "errorCode": "4007", + "errorInfo": {}, + "errorType": "IncompatibleNozzleConfiguration", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [ + { + "createdAt": "TIMESTAMP", + "detail": "KeyError: 'G12'", + "errorCode": "4000", + "errorInfo": { + "args": "('G12',)", + "class": "KeyError", + "traceback": " File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in build\n back_left_row_index, back_left_column_index = _row_col_indices_for_nozzle(\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in _row_col_indices_for_nozzle\n return _row_or_col_index_for_nozzle(rows, nozzle), _row_or_col_index_for_nozzle(\n\n File \"/usr/local/lib/python3.10/site-packages/opentrons/hardware_control/nozzle_manager.py\", line N, in _row_or_col_index_for_nozzle\n raise KeyError(nozzle)\n" + }, + "errorType": "PythonException", + "id": "UUID", + "isDefined": false, + "wrappedErrors": [] + } + ] + } + ] + } + ] + } + ], + "files": [ + { + "name": "Flex_X_v2_20_96_and_8_Overrides_InvalidConfigs_Override_eight_partial_column_bottom_right.py", + "role": "main" + } + ], + "labware": [ + { + "definitionUri": "opentrons/opentrons_flex_96_tiprack_1000ul/1", + "id": "UUID", + "loadName": "opentrons_flex_96_tiprack_1000ul", + "location": { + "slotName": "B2" + } + } + ], + "liquids": [], + "metadata": { + "description": "oooo", + "protocolName": "Invalid tip configs that should error" + }, + "modules": [], + "pipettes": [ + { + "id": "UUID", + "mount": "left", + "pipetteName": "p1000_multi_flex" + } + ], + "result": "not-ok", + "robotType": "OT-3 Standard", + "runTimeParameters": [] +} diff --git a/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[d2c818bf00][Flex_S_v2_20_P50_LPD].json b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[d2c818bf00][Flex_S_v2_20_P50_LPD].json new file mode 100644 index 00000000000..0e914568a92 --- /dev/null +++ b/analyses-snapshot-testing/tests/__snapshots__/analyses_snapshot_test/test_analysis_snapshot[d2c818bf00][Flex_S_v2_20_P50_LPD].json @@ -0,0 +1,5125 @@ +{ + "commands": [ + { + "commandType": "home", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "50c7ae73a4e3f7129874f39dfb514803", + "notes": [], + "params": {}, + "result": {}, + "startedAt": "TIMESTAMP", + "status": "succeeded" + }, + { + "commandType": "loadLabware", + "completedAt": "TIMESTAMP", + "createdAt": "TIMESTAMP", + "id": "UUID", + "key": "73d9d4d55ae8466f3a793ceb70545fa5", + "notes": [], + "params": { + "loadName": "opentrons_flex_96_tiprack_1000ul", + "location": { + "slotName": "A2" + }, + "namespace": "opentrons", + "version": 1 + }, + "result": { + "definition": { + "allowedRoles": [], + "brand": { + "brand": "Opentrons", + "brandId": [] + }, + "cornerOffsetFromSlot": { + "x": 0, + "y": 0, + "z": 0 + }, + "dimensions": { + "xDimension": 127.75, + "yDimension": 85.75, + "zDimension": 99 + }, + "gripForce": 16.0, + "gripHeightFromLabwareBottom": 23.9, + "gripperOffsets": {}, + "groups": [ + { + "metadata": {}, + "wells": [ + "A1", + "A10", + "A11", + "A12", + "A2", + "A3", + "A4", + "A5", + "A6", + "A7", + "A8", + "A9", + "B1", + "B10", + "B11", + "B12", + "B2", + "B3", + "B4", + "B5", + "B6", + "B7", + "B8", + "B9", + "C1", + "C10", + "C11", + "C12", + "C2", + "C3", + "C4", + "C5", + "C6", + "C7", + "C8", + "C9", + "D1", + "D10", + "D11", + "D12", + "D2", + "D3", + "D4", + "D5", + "D6", + "D7", + "D8", + "D9", + "E1", + "E10", + "E11", + "E12", + "E2", + "E3", + "E4", + "E5", + "E6", + "E7", + "E8", + "E9", + "F1", + "F10", + 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"opentrons/opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical/1", + "id": "UUID", + "loadName": "opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical", + "location": { + "slotName": "C1" + } + }, + { + "definitionUri": "opentrons/nest_96_wellplate_100ul_pcr_full_skirt/2", + "id": "UUID", + "loadName": "nest_96_wellplate_100ul_pcr_full_skirt", + "location": { + "slotName": "D2" + } + }, + { + "definitionUri": "opentrons/nest_96_wellplate_100ul_pcr_full_skirt/2", + "id": "UUID", + "loadName": "nest_96_wellplate_100ul_pcr_full_skirt", + "location": { + "slotName": "D3" + } + } + ], + "liquids": [], + "metadata": { + "description": "oooo", + "protocolName": "Too tall labware on pickup tip" + }, + "modules": [], + "pipettes": [ + { + "id": "UUID", + "mount": "left", + "pipetteName": "p1000_96" + } + ], + "result": "not-ok", + "robotType": "OT-3 Standard", + "runTimeParameters": [] +}