Long reads?

[ghost]

Hello,

Can the test reads be long reads? In theory I don't see why not, but do you think the results would be as robust? I am just worried the Kmer counting does something wild.

Thanks

Alex

[ldenti]

Hi,
although we never tested it on long reads, I'd assume that results won't be reliable. Maybe with PacBio HiFi reads (or corrected long reads) it may work, but I'm not that sure. As you correctly pointed out, the problem is the kmer counting: odd kmer counts may result in poor genotyping accuracy.

Best,

[ghost]

Thanks for your answer, and sorry I had missed it. You confirm my worries, so I will not use it with the long reads but with the illumina only. Thanks again